Tissue chip mold

Abstract

According to the tissue chip mold, a plurality of circular through holes are formed in the bottom of a first embedding mold box, the diameter of a cylindrical bolt corresponds to the inner diameter of each circular through hole, and the internal size and shape of a mold box bottom support correspond to the external size and shape of the first embedding mold box and the external size and shape of a second embedding mold box; the cylindrical plug pin is used for being inserted into the circular through hole of the first embedding mold box, the mold box bottom support is used for bearing the first embedding mold box in which the cylindrical plug pin is inserted, so that a paraffin model is poured in the first embedding mold box, and the second embedding mold box is used for bearing a paraffin model filled with a tissue sample, so that the paraffin model and the tissue sample are integrated. The hole size and arrangement position of the blank wax block can be selectively arranged through the embedding mold box and the cylindrical bolt, random arrangement can be conveniently carried out according to actual requirements, the structure is simple, use is convenient, the precision is higher than that of manual punching, and the cost is far lower than that of punching controlled by a small milling machine or CNC.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological experiments, and particularly relates to a tissue chip mold. BACKGROUND

[0002] Tissue Chip, also known as Tissue Microarray (TMA), is a modern scientific method combining morphology and molecular biology. Since its inception in 1998, it has been widely used in biomedical research due to its large-scale, high-throughput and standardized characteristics. This technology arranges dozens to thousands of micro-tissue samples in a regular manner on a single slide to form a microarray format, enabling researchers to simultaneously detect and analyze the expression of specific genes or proteins in multiple tissue samples. The tissue chip technology not only improves experimental efficiency, but also ensures the consistency of all samples under experimental conditions, making the comparison between tissues more reliable. This technology provides strong support for molecular diagnosis, treatment decision and prognosis evaluation of diseases, and has great potential in the fields of tumor research, clinical research and drug development.

[0003] The paraffin embedding method is to embed selected donor tissues in wax blocks, use a special sampling needle to extract micro-tissue samples from the wax blocks, and arrange these samples in order on a pre-prepared receiving wax block, and finally fuse into a unified wax block for sectioning. This method is suitable for most tissue samples, but has limitations when dealing with some tissues that may damage RNA or affect antigen-antibody binding. Moreover, when making a blank wax block, the manual punching precision is low, and the small milling machine or CNC controlled punching requires high equipment requirements, and the mold punching is not flexible enough. CONTENT OF THE UTILITY MODEL

[0004] The embodiment of the present application provides a tissue chip mold to solve the problem of low manual punching precision of the blank wax block in the prior art paraffin embedding group, high equipment requirement of the small milling machine or CNC controlled punching, and not flexible enough of the mold punching.

[0005] In one aspect, the embodiment of the present application provides a tissue chip mold, comprising:

[0006] The first embedding mold box, the second embedding mold box, the cylindrical plug and the mold box bottom support, the bottom of the first embedding mold box is provided with a plurality of circular through holes, the diameter of the cylindrical plug corresponds to the inner diameter of the circular through hole, the inside of the mold box bottom support corresponds to the outside size and shape of the first embedding mold box and the second embedding mold box, the cylindrical plug is used for inserting into the circular through hole of the first embedding mold box, the mold box bottom support is used for supporting the first embedding mold box inserted with the cylindrical plug, so as to pour the paraffin model in the first embedding mold box, and the second embedding mold box is used for supporting the paraffin model filled with the tissue sample, so as to integrate the paraffin model and the tissue sample.

[0007] In a possible implementation, one end of the cylindrical plug is provided with a square base, the square base is larger than the circular through hole at the bottom of the first embedding mold box, and a plurality of square bases are connected into a piece after a plurality of cylindrical plugs are inserted into the circular through hole at the bottom of the first embedding mold box.

[0008] In a possible implementation, the embedding frame bottom support is further included, the longitudinal section of the embedding frame bottom support is trapezoidal, and the embedding frame bottom support is used for covering the first embedding mold box in which the paraffin model is poured.

[0009] In a possible implementation, the top of the first embedding mold box and the second embedding mold box is provided with a platform type slot.

[0010] In a possible implementation, the bottom of the embedding frame bottom support corresponds to the size of the platform type slot of the first embedding mold box and the second embedding mold box.

[0011] In a possible implementation, the first embedding mold box, the second embedding mold box and the cylindrical plug are made of a heat-conducting metal material with a melting point higher than that of paraffin.

[0012] The tissue chip mold in the application has the following advantages:

[0013] (1) The hollow size and arrangement position of the blank wax block can be selected and arranged through the embedding mold box and the cylindrical plug, so that random setting can be conveniently performed according to actual needs.

[0014] (2) The structure is simple and convenient to use, the precision is higher than that of manual punching, and the cost is much lower than that of a small milling machine or CNC controlled punching. BRIEF DESCRIPTION OF DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or prior art description will be briefly introduced. Obviously, the drawings in the following description only represent some of the embodiments of the present application, and for those skilled in the art, other drawings can be obtained without creative labor on the basis of these drawings.

[0016] Figure 1 A disassembled sectional view of a tissue chip mold provided by the embodiment of the present application;

[0017] Figure 2 A three-dimensional view of a tissue chip mold provided by the embodiment of the present application;

[0018] Figure 3 A combined sectional view of a tissue chip mold provided by the embodiment of the present application;

[0019] Figure 4 A model pouring sectional view of a tissue chip mold provided by the embodiment of the present application;

[0020] Figure 5 A model disassembly view of a tissue chip mold provided by the embodiment of the present application;

[0021] Figure 6 A sample combination view of a tissue chip mold provided by the embodiment of the present application;

[0022] Figure 7 A sample fusion view of a tissue chip mold provided by the embodiment of the present application.

[0023] Explanation of the reference numerals in the drawings: 1, first embedding mold box; 2, cylindrical bolt; 3, second embedding mold box; 4, mold box bottom support; 5, embedding frame base; 6, paraffin; 7, tissue sample. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments only represent some of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0025] Figure 1 A signal transmission view of a tissue chip mold provided by the embodiment of the present application; the embodiment of the present application provides a tissue chip mold, which comprises:

[0026] The first embedding mold box 1, the second embedding mold box 3, the cylindrical plug 2 and the mold box bottom support 4, the first embedding mold box 1 is provided with a plurality of circular through holes at the bottom, the cylindrical plug 2 is provided with a diameter corresponding to the inner diameter of the circular through hole, the mold box bottom support 4 is provided with an internal size and shape corresponding to the external size and shape of the first embedding mold box 1 and the second embedding mold box 3, the cylindrical plug 2 is used for plugging into the circular through hole of the first embedding mold box 1, the mold box bottom support 4 is used for supporting the first embedding mold box 1 with the cylindrical plug 2 plugged in, so as to cast a paraffin model in the first embedding mold box 1, and the second embedding mold box 3 is used for supporting the paraffin model filled with the tissue sample 7, so as to fuse the paraffin model and the tissue sample 7 into one, further comprising an embedding frame base 5, the longitudinal section of the embedding frame base 5 is trapezoidal, the embedding frame base 5 is used for covering the first embedding mold box 1 with the paraffin model casted, the top of the first embedding mold box 1 and the second embedding mold box 3 is provided with a platform type slot, the bottom of the embedding frame base 5 is provided with a size corresponding to the platform type slot of the first embedding mold box 1 and the second embedding mold box 3, and the first embedding mold box 1, the second embedding mold box 3 and the cylindrical plug 2 are made of a heat-conducting metal material with a melting point higher than that of paraffin.

[0027] As an example, Figure 2 , 3 , 4, 5, 6, 7, the first embedding mold box 1 and the second embedding mold box 3 are pathological paraffin embedding mold boxes with the same specifications, wherein the first embedding mold box 1 is provided with a plurality of circular through holes arranged in a regular rectangular array at the bottom, for the cylindrical plug 2 to be inserted. In use, the cylindrical plug 2 is arranged on the first embedding mold box 1 according to experimental requirements, and then the mold box bottom support 4 is used to support the first embedding mold box 1 and the cylindrical plug 2, so as to form a complete mold base. Then paraffin 6 is injected into the complete mold base until the platform type slot position of the first embedding mold box 1, and then the embedding frame base 5 is covered, until the paraffin 6 solidifies. Then the mold box bottom support 4 is removed, and then the cylindrical plug 2 is withdrawn one by one, and the paraffin 6 formed into a hole-containing blank paraffin model is taken out through the embedding frame base 5. The tissue sample 7 is made into a cylinder with the same size as the cylindrical plug 2, and then filled into the blank paraffin model. Then the paraffin model filled with the tissue sample 7 is put back into the second embedding mold box 3 together with the embedding frame base 5, and the blank paraffin model is fused with the tissue sample 7 by slightly heating the second embedding mold box 3, so as to obtain a complete paraffin embedding.

[0028] In a possible embodiment, one end of the cylindrical plug 2 is provided with a square base, the size of the square base corresponds to the arrangement of the through holes at the bottom of the first embedding mold box 1, and after a plurality of cylindrical plugs 2 are inserted into the through holes at the bottom of the first embedding mold box 1, the square bases of the plurality of cylindrical plugs 2 are tightly connected into a piece.

[0029] For example, the square base closely contacts the first embedding mold box 1 in use, which prevents the displacement of the cylindrical pin 2 during the production of the paraffin embedding, so as to prevent the irregular arrangement of the holes.

[0030] Although preferred embodiments of the application have been described herein, changes and modifications can be suggested to one skilled in the art and are intended to be encompassed within the scope of the application. It is the intent, therefore, to be limited only as indicated by the scope of the claims appended hereto.

[0031] Obviously, numerous modifications and variations of the present application are possible in light of the above teachings. It is therefore to be understood that within the scope of the appended claims and their equivalents, the application can be practiced otherwise than as specifically described.

Claims

1. A tissue chip mold characterized by, The application relates to a embedding mold box, which comprises a first embedding mold box (1), a second embedding mold box (3), a cylindrical plug (2) and a mold box bottom support (4), the bottom of the first embedding mold box (1) is provided with a plurality of circular through holes, the diameter of the cylindrical plug (2) corresponds to the inner diameter of the circular through holes, the inner size and shape of the mold box bottom support (4) correspond to the outer size and shape of the first embedding mold box (1) and the second embedding mold box (3), the cylindrical plug (2) is used for being inserted into the circular through holes of the first embedding mold box (1), the mold box bottom support (4) is used for supporting the first embedding mold box (1) with the cylindrical plug (2) inserted, so that a paraffin model is cast in the first embedding mold box (1), and the second embedding mold box (3) is used for supporting a paraffin model filled with a tissue sample (7) and fusing the paraffin model and the tissue sample (7) into one. The cylindrical plug (2) is provided with a square base at one end, the square base is larger than the circular through holes at the bottom of the first embedding mold box (1), and a plurality of square bases are connected into a sheet after a plurality of cylindrical plugs (2) are inserted into the circular through holes at the bottom of the first embedding mold box (1).

2. The tissue chip mold of claim 1, wherein, The embedding mold box further comprises an embedding frame base (5), the longitudinal section of the embedding frame base (5) is trapezoidal, and the embedding frame base (5) is used for covering the first embedding mold box (1) with the cast paraffin model.

3. The tissue chip mold of claim 1, wherein, The top of the first embedding mold box (1) and the second embedding mold box (3) is provided with a platform type slot.

4. The tissue chip mold of claim 3, wherein, The bottom of the embedding frame base (5) corresponds to the size of the platform type slot of the first embedding mold box (1) and the second embedding mold box (3).

5. The tissue chip mold of claim 4, wherein, The first embedding mold box (1), the second embedding mold box (3) and the cylindrical plug (2) are made of a heat-conducting metal material with a melting point higher than that of paraffin.

6. A tissue chip mold according to claim 5, wherein ​

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