Micro-fluidic chip for detecting multiple pathogenic bacteria
By designing a microfluidic chip and utilizing the aptamer reaction of gold nanoparticles, rapid qualitative detection of foodborne pathogens is achieved, solving the problems of long cycle time of conventional detection methods and high cost of immunological detection methods, and realizing rapid, sensitive and low-cost multi-index detection.
Patent Information
- Application Number
- CN202422698320.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-06
- Publication Date
- 2025-12-12
- Estimated Expiration
- 2034-11-06
AI Technical Summary
In existing technologies, conventional detection methods have long detection cycles and low costs, but slow detection speeds; immunological detection methods are fast but expensive.
Design a microfluidic chip comprising a chip body and a glass substrate, with a sample dispensing port, a buffer channel and a shunt zone, to achieve qualitative detection of foodborne pathogens by utilizing aptamer reactions on gold nanoparticles, and to determine the results by color changes, integrating bacterial proliferation and detection processes.
It enables rapid, sensitive, and low-cost detection of multiple pathogenic bacteria, reduces the risk of cross-contamination, simplifies operation procedures, and achieves fully automated detection.
Smart Images
Figure CN223660088U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to micro -fluidic chip technical field, especially a kind of micro -fluidic chip for a variety of pathogenic bacteria detection. BACKGROUND
[0002] With the importance of food safety, the rapid, sensitive and high-throughput detection and analysis of food contaminants are increasingly important. Micro total analysis system integrates different functional chips on a microfluidic chip, enabling automated sample transport, rapid reaction, accurate detection and timely cleaning on the chip, thereby greatly improving analysis efficiency. Microfluidic chip technology has good application prospects in detecting foodborne pathogenic bacteria. The accurate control of fluid and real-time rapid detection of foodborne pathogenic bacteria are key to detecting foodborne pathogenic bacteria. Microfluidic chip is a hot area of micro total analysis system development. It uses a chip as an operation platform and combines with biological, chemical and drug screening technologies to complete the entire process including reagent loading, separation, reaction, detection and other processes. With the rapid development of biochip technology, microfluidic chips play an increasingly important role in life science, analytical chemistry and biomedical fields. To efficiently, rapidly and high-throughput detect samples, the chip requires multiple reaction wells and an effective sample delivery method to deliver samples to each reaction well.
[0003] Currently, the reported detection methods for foodborne bacteria include:
[0004] 1. Conventional detection methods are simple to operate and have low detection costs, but have the disadvantage of long detection period;
[0005] 2. Immunological detection methods detect foodborne bacteria through specific antigen-antibody reactions, which are fast but have high detection costs;
[0006] 3. Molecular biology detection methods such as PCR technology have strong specificity but require high operating skills for detection equipment;
[0007] 4. Fluorescence or electrochemical-based biosensor detection methods have fast detection speed and high sensitivity, but are expensive and not suitable for widespread promotion.
[0008] Therefore, we propose a detection method for foodborne bacteria that is fast, sensitive and low-cost. Utility model content
[0009] (I) Technical problem solved
[0010] In view of the defects of the prior art, the utility model provides a micro fluidic chip for a variety of pathogenic bacteria detection, solve the routine detection method although simple operation, detection cost is lower, but have the disadvantage of long detection period and immunology detection method, through the utilization antigen antibody's specific reaction carries out the detection of foodborne bacteria, although detection speed is faster, but have the disadvantage of high detection cost problem.
[0011] (II) Technical solutions
[0012] In order to realize above -mentioned purpose, the utility model discloses a micro fluidic chip for a variety of pathogenic bacteria detection through following technical schemes: a micro fluidic chip for a variety of pathogenic bacteria detection, including chip main part, the glass substrate is arranged below chip main part, the chip main part upper surface is opened with sample addition port one and sample addition port two, the chip main part upper surface is opened with a group of detection area, the chip main part upper surface is opened with sample addition buffer channel.
[0013] As a preferred technical scheme of the utility model, the chip main part upper surface is opened with a group of buffer channels, and the sample addition buffer channel and the group of buffer channels are both serpentine curved.
[0014] As a preferred technical scheme of the utility model, the chip main part upper surface is opened with a group of buffer channels, and the sample addition buffer channel and the group of buffer channels are both serpentine curved.
[0015] As a preferred technical scheme of the utility model, the chip main part upper surface is opened with a group of sample addition port three.
[0016] As a preferred technical scheme of the utility model, the chip main part upper surface is opened with a group of culture area.
[0017] (III) Beneficial effects
[0018] 1, before detection, the aptamer of detection item is coated on the gold nanoparticle, the chip can detect four different indexes simultaneously, after adding sample, the sample will proliferate in four culture areas, then the to-be-tested liquid is driven to flow through the detection area by external air pump, and the gold-nanoparticle-aptamer coated on the detection area fully reacts, in the experiment, the specific aptamer of foodborne pathogenic bacteria is fixed on the surface of gold nanoparticle, then gold nanoparticle is induced to gather through NaCl solution, because the surface of gold nanoparticle is wrapped by aptamer at this time, when it gathers, the solution is red, when the foodborne pathogenic bacteria is added in the to-be-tested liquid, the foodborne pathogenic bacteria will have specific reaction with its aptamer, so that gold nanoparticle is released, and the solution is blue after gathering, the qualitative detection of foodborne pathogenic bacteria can be realized through color change, the whole realizes the integrated operation of bacterial proliferation and detection, realizes the multi-index detection of sample, realizes the partition and reduces pollution, in the detection area, the aptamer is coated on the gold nanoparticle before detection, and this is more conducive to realizing the full automation of the detection process.
[0019] 2、The whole body provides a stable platform through the glass substrate during use, sample adding buffer channels are connected with sample adding port one, sample adding port two, sample adding port three and a split flow area to buffer sample flow, the sample adding buffer channels and a group of buffer channels are both snake-shaped and curved, and uniform distribution is ensured, the split flow area distributes the sample to different detection areas to realize simultaneous detection of multiple indexes, the risk of cross contamination is reduced through partition design, the bacterial proliferation and detection process are integrated, the operation steps are simplified, and the whole body realizes integrated detection, a bacterial culture module is added, samples and culture media can be added from different inlets at the same time to realize detection after bacterial culture, and thus low-concentration sample interpretation can be realized. BRIEF DESCRIPTION OF DRAWINGS
[0020] The above description is only a summary of the technical scheme of the present application, in order to more clearly understand the technical means of the present application, and the content of the specification can be implemented, the following will be described in detail with the preferred embodiment of the present application and the accompanying drawings.
[0021] Fig. 1 It is the overall structure diagram of the present application;
[0022] Fig. 2 It is the chip main structure diagram in the present application;
[0023] Fig. 3 It is the glass substrate structure diagram in the present application.
[0024] Legend: 1, chip main body; 2, glass substrate; 3, sample adding port one; 4, sample adding port two; 5, sample adding buffer channel; 6, split flow area; 7, buffer channel; 8, sample adding port three; 9, culture area; 10, detection area. DETAILED DESCRIPTION
[0025] The microfluidic chip for detecting multiple pathogenic bacteria provided by the embodiment of the present application effectively solves the problems that the conventional detection method has the disadvantages of long detection period although it is simple to operate and has low detection cost, and the immunological detection method has the disadvantage of high detection cost although it has fast detection speed, the aptamer of the detection item is coated on the gold nanoparticles before detection, the chip can detect four different indexes at the same time, after adding the sample, the sample will proliferate in the four culture areas 9, then the to-be-detected liquid is driven to flow through the nanogold-aptamer coated on the detection area 10 by an external air pump, the specific aptamer of the foodborne pathogenic bacteria is fixed on the surface of the gold nanoparticles during the experiment, then the gold nanoparticles are induced to aggregate by NaCl solution, and the surface of the gold nanoparticles is wrapped by the aptamer at this time.
[0026] EMBODIMENT
[0027] As Figs. 1 to 3 shown, the technical scheme in the embodiment of the application effectively solves the problems that the conventional detection method has the disadvantages of long detection period although it is simple to operate and low in detection cost, and the immunological detection method has the disadvantage of high detection cost although it is fast in detection speed, and the overall idea is as follows:
[0028] In view of the problems in the prior art, the utility model provides a micro fluidic chip for detecting multiple pathogenic bacteria, including chip main part 1, chip main part 1 below is provided with glass base 2, the upper surface of chip main part 1 is provided with sample adding port one 3 and sample adding port two 4, a group of detection area 10 is opened in the upper surface of chip main part 1, the upper surface of chip main part 1 is provided with sample adding buffer channel 5, before detection, the aptamer of detection item is coated on gold nano particle, the chip can detect four different indexes simultaneously, after adding sample, the sample will proliferate in four culture areas 9, then the to-be-tested liquid is driven to flow through the nanometer gold-aptamer coated on the detection area 10 in advance by external air pump and fully reacts, the specific aptamer of foodborne pathogenic bacteria is fixed on the surface of gold nano particle during experiment, then gold nano particle is induced to gather by NaCl solution, since the surface of gold nano particle is wrapped by aptamer at this time, when it gathers, the solution is red, when foodborne pathogenic bacteria is added in the to-be-tested liquid, foodborne pathogenic bacteria will have specific reaction with its aptamer, so that gold nano particle is released.
[0029] The upper surface of chip main part 1 is provided with a group of buffer channels 7, the sample adding buffer channel 5 and the group of buffer channels 7 are both provided in the shape of snake curve, the upper surface of chip main part 1 is provided with shunt area 6, the shunt area 6 is communicated with the sample adding buffer channel 5 and the group of buffer channels 7 respectively, the solution is blue after gathering, the qualitative detection of foodborne pathogenic bacteria can be realized by color change, the whole realizes the integrated operation of bacterial proliferation and detection, realizes the multi-index detection of sample, realizes the reduction of pollution in partition, in the detection area 10, the aptamer is coated on nanometer gold particle before detection, which is more conducive to realizing the full automation of detection process.
[0030] A group of sample adding ports three 8 are arranged on the upper surface of the chip body 1, a group of culture areas 9 are arranged on the upper surface of the chip body 1, and in the use process, a stable platform is provided through the glass base 2, the sample adding buffer channel 5 is connected with the sample adding port one 3, the sample adding port two 4, the sample adding port three 8 and the shunt area 6 to buffer the sample flow, the sample adding buffer channel 5 and the group of buffer channels 7 are both arranged in a snake shape and ensure uniform distribution, the shunt area 6 distributes the sample to different detection areas to realize simultaneous detection of multiple indexes, the risk of cross contamination is reduced through the partition design, the bacterial proliferation and detection process are integrated, the operation steps are simplified, and integrated detection is realized, the bacterial culture module is added, the sample and the culture medium can be added from different inlets at the same time, detection is realized after bacterial culture, and thus low-concentration sample interpretation can be realized.
[0031] Working principle:
[0032] Before detection, the aptamer of the detection item is coated on the gold nanoparticles, the chip can simultaneously detect four different indexes, after sample adding, the sample is proliferated in the four culture areas 9, and then the to-be-detected liquid is driven to flow through the detection area 10 pre-coated with the nano-gold-aptamer to fully react, in the experiment, the specific aptamer of the foodborne pathogenic bacteria is fixed on the surface of the gold nanoparticles, then the gold nanoparticles are induced to gather through a NaCl solution, because the surface of the gold nanoparticles is wrapped by the aptamer at this time, when the gold nanoparticles gather, the solution is red, when the foodborne pathogenic bacteria is added in the to-be-detected liquid, the foodborne pathogenic bacteria specifically reacts with the aptamer, so that the gold nanoparticles are released, the solution is blue after gathering, qualitative detection of the foodborne pathogenic bacteria can be realized through color change, integrated bacterial proliferation and detection operation are realized, multiple index detection of the sample is realized, pollution is reduced through partition, in the detection area 10, the aptamer is coated on the nano-gold particles before detection, which is more conducive to realizing full automation of the detection process, in the use process, a stable platform is provided through the glass base 2, the sample adding buffer channel 5 is connected with the sample adding port one 3, the sample adding port two 4, the sample adding port three 8 and the shunt area 6 to buffer the sample flow, the sample adding buffer channel 5 and the group of buffer channels 7 are both arranged in a snake shape and ensure uniform distribution, the shunt area 6 distributes the sample to different detection areas to realize simultaneous detection of multiple indexes, the risk of cross contamination is reduced through the partition design, the bacterial proliferation and detection process are integrated, the operation steps are simplified, and integrated detection is realized, the bacterial culture module is added, the sample and the culture medium can be added from different inlets at the same time, detection is realized after bacterial culture, and thus low-concentration sample interpretation can be realized.
[0033] Finally, it should be noted that: apparently, the above embodiments are merely examples for clearly illustrating the utility model, and are not limited to the implementation. For ordinary skilled persons in the art, other different forms of changes or variations can be made on the basis of the above description. Here, it is not necessary and impossible to exhaust all the implementation. The obvious changes or variations derived therefrom are still within the protection scope of the utility model.
Claims
1. A microfluidic chip for detecting a plurality of pathogenic bacteria, comprising a chip main body (1), characterized in that, The chip body (1) is provided below with a glass substrate (2), the upper surface of the chip body (1) is provided with a sample adding port one (3) and a sample adding port two (4), a group of detection zones (10) are arranged on the upper surface of the chip body (1), a sample adding buffer channel (5) is arranged on the upper surface of the chip body (1), a group of buffer channels (7) are arranged on the upper surface of the chip body (1), the sample adding buffer channel (5) and the group of buffer channels (7) are both arranged in a serpentine shape, a shunt zone (6) is arranged on the upper surface of the chip body (1), the shunt zone (6) is in communication with the sample adding buffer channel (5) and the group of buffer channels (7) respectively, a group of sample adding port threes (8) are arranged on the upper surface of the chip body (1), and a group of culture zones (9) are arranged on the upper surface of the chip body (1).