Insect olfaction behavior experimental device

Abstract

The utility model relates to an insect olfaction behavior experimental device, and relates to the technical field of insect behavior research. According to the main technical scheme, the insect olfactory behavior experimental device comprises a first chamber and an experimental chamber. Wherein the first chamber is used for breeding insects; the experiment chamber is used for performing an insect olfactory behavior experiment; the experiment chamber comprises a second chamber which is used for providing free activity space for the insects so as to be used for the olfactory behavior experiment of the insects; wherein the first chamber and the second chamber are separated by a movable door; wherein the movable door is opened, so that the first chamber is communicated with the second chamber, and the insects bred in the first chamber are released into the second chamber. The device is mainly used for solving the technical problems that in the prior art, when insects are taken into an experimental device, mosquitoes can be damaged, and experimental results are affected, the device integrates the olfactometer and the wind tunnel, and wind tunnel testing and selection behavior testing can be achieved at the same time.

Description

TECHNICAL FIELD

[0001] The utility model relates to a kind of insect behavior research technical field, in particular to a kind of insect olfactory behavior experimental device. BACKGROUND

[0002] Olfaction is a sharp weapon for insect survival, and plays an important role in insect foraging, mating, oviposition and avoiding natural enemies. Insects rely on sensitive olfaction to perceive chemical information in the environment. In the process of olfactory recognition, odor receptors play a key role. They can convert external chemical signals into bioelectric signals after being activated, thereby mediating the corresponding behavioral responses of insects.

[0003] By analyzing the olfactory behavior of insects, developing insect olfactory behavior modulators based on olfactory targets is a globally recognized green control technology. It has the advantages of high biological sensitivity, strong selectivity and environmental friendliness, and can achieve long-term effective control of pests. It has become an important direction of current research.

[0004] Unlike the neural circuit of traditional animal olfactory system, mosquitoes have a special olfactory system with "automatic fault protection device", namely multi-receptor olfactory system. That is, a single olfactory neuron of mosquito has multiple types of chemical receptors, which can detect different odors, ensuring that they can always smell the odor of host and accurately locate the host. When people try to prevent mosquitoes from biting by blocking certain receptors, mosquitoes can still use other receptors to easily identify hosts, making it more difficult to confuse mosquito olfaction than previously thought. This is also the difficulty in developing mosquito repellents and attractants.

[0005] Therefore, developing a convenient, economical and efficient insect olfactory behavior experimental device is a powerful guarantee for creating efficient and specific green insect behavior modulators. It is also necessary for in-depth research on the principles of mosquito-borne virus transmission in nature, and for the development of new prevention and control strategies to block the large-scale spread of viruses worldwide and protect human public health.

[0006] In the prior art, the living organism determination experimental device for insect olfactory behavior mainly includes olfactometer and olfactory wind tunnel. Among them, the olfactometer mainly includes Y-type, four-arm type and multi-channel type. The olfactory wind tunnel, also known as insect wind tunnel test system, is a carefully designed tunnel or pipeline, mainly composed of air supply system, observation area, collection device (branch arm) and the like. Its core value lies in converting theoretical models into observable and quantifiable experimental data. In the field of insect chemical ecology, wind tunnel test is applied in a specially designed rectangular space, which maintains air circulation. Scientists observe the behavioral response of living insects to specific odor substances to explore the olfactory orientation mechanism of insects and their recognition and response ability to chemical signals.

[0007] However, the existing insect olfactory behavior determination experimental device at least has the following problems:

[0008] 1) The prior art first adopts an insect taking device (such as a mosquito suction device) to take insects into the experimental device. For example, when a mosquito suction device is used to take mosquitoes, the mosquitoes are mainly sucked into the mosquito suction device by using wind force, and then released into the determination experimental device. However, in this process, the mosquito suction device will damage the mosquitoes, seriously affect the activity of the mosquitoes, affect the experimental results, and even some mosquitoes will directly die in this process.

[0009] 2) The number of insects for one-time experiment in the prior art is small, and it is difficult to meet the demand of single large-scale experiment.

[0010] 3) The prior art mainly counts the number of insects after selection to determine the attraction or repulsion effect, and cannot record the flight behavior of insects facing different odors.

[0011] 4) The prior art needs more human operation steps, and the operation difference between different experimenters will lead to the difference of results, and consumes manpower; the diurnal rhythm of different types of insects is different, and most insects are active at night or early morning, which is different from the regular working time of humans. The conventional olfactory behavior experimental device needs experimenters to work outside the 8-hour working time.

[0012] 5) The temperature and humidity in the experimental device are not clear, and the existing device mainly uses the environmental conditions outside the experimental device as the experimental parameters, but the temperature and humidity outside the experimental device are not equal to the physical conditions in the device, which will lead to the difference of insect activity and the repeatability of experimental results. Practical new type content

[0013] Therefore, the utility model provides a mosquito olfactory behavior experimental device, and the main purpose is to solve the technical problem that the prior art will damage mosquitoes in the process of taking insects into the experimental device and affect the experimental results.

[0014] In order to achieve the above purpose, the utility model mainly provides the following technical scheme:

[0015] On the one hand, the utility model embodiment provides an insect olfactory behavior experimental device, wherein the insect olfactory behavior experimental device comprises:

[0016] A first chamber for breeding insects;

[0017] An experimental chamber for conducting insect olfactory behavior experiments; wherein the experimental chamber comprises a second chamber for providing free activity space for insects for insect olfactory behavior experiments;

[0018] The first chamber and the second chamber are separated by a movable door; the first chamber and the second chamber are communicated by opening the movable door to release the insects bred in the first chamber into the second chamber.

[0019] Preferably, the first chamber is used for adult emergence, preferably also for larva hatching; and / or the first chamber is provided with temperature control device, humidity control device, temperature detection device and humidity detection device to provide a temperature and humidity environment suitable for breeding insects for the first chamber; and / or a timer is installed on the outside of the first chamber to record the time of larva hatching and / or adult emergence; and / or a timer is installed on the outside of the second chamber to record the release time of releasing the insects bred in the first chamber into the second chamber; and / or the first chamber is provided with a chamber door for opening the first chamber; preferably, the chamber door is a mesh door for air circulation between the first chamber and the outside; preferably, the mesh of the chamber door is 16-60 mesh.

[0020] Preferably, the movable door is a mesh air door; the mesh of the movable door is 16-60 mesh.

[0021] Preferably, the movable door is an upward pull door, wherein the first chamber and the second chamber are communicated by moving the movable door upward by pulling upward.

[0022] Preferably, the experimental chamber further comprises:

[0023] A plurality of test chambers, each of which is in communication with the second chamber; wherein the test chamber comprises at least one first test chamber for placing experimental samples and at least one second test chamber for placing control samples; preferably, the test chamber comprises a plurality of first test chambers for placing different experimental samples.

[0024] An aeration device comprising an aeration pipeline; wherein the aeration pipeline is connected with the test chamber for introducing gas into the test chamber.

[0025] Preferably, a trap device is provided in the test chamber to prevent the insects entering the test chamber from returning to the second chamber; preferably, the trap device is a funnel net structure; preferably, the large end of the funnel net structure is arranged close to the second chamber and the small end is arranged away from the second chamber; preferably, the large end of the funnel net structure is located at the inlet end of the test chamber; preferably, a set space is left between the small end of the funnel net structure and the air inlet end of the test chamber to place experimental samples or control samples and accommodate insects; wherein the inlet end and the air inlet end of the test chamber are oppositely arranged.

[0026] Preferably, the ventilation device is consistent with the number of test chambers and one-to-one correspondence; and / or the ventilation device further comprises: a gas generating device, a purification device, a humidifying device; wherein the gas generating device, the purification device, the humidifying device, the ventilation pipeline and the test chamber are sequentially communicated; preferably, the purification device comprises an activated carbon purification device; preferably, the ventilation device further comprises a flowmeter; preferably, the flowmeter is connected to the pipeline between the gas generating device and the purification device; and / or the movable door is located on the first side of the second chamber, and the test chamber is connected with the second side of the second chamber; preferably, the first side and the second side are oppositely arranged; preferably, a plurality of mounting holes are formed on the second side of the second chamber; the number of test chambers is consistent with the number of mounting holes and one-to-one correspondence, and the test chamber is mounted at the corresponding mounting hole.

[0027] Preferably, the insect olfactory behavior experiment device further comprises:

[0028] The monitoring device comprises a first monitoring device and a second monitoring device; wherein the first monitoring device is used for recording the flight behavior of the insects in the second chamber, and the second monitoring device is used for recording the selection behavior of the insects in the second chamber, so as to form video data for data analysis.

[0029] Compared with the prior art, the mosquito olfactory behavior experiment device has at least the following beneficial effects:

[0030] The embodiment provides an insect olfactory behavior experiment device, a first chamber for breeding insects and an experiment chamber for insect olfactory behavior experiment are arranged, and the first chamber and a second chamber in the experiment chamber are separated by a movable door, so that when the insect olfactory behavior experiment is performed, the first chamber and the second chamber are communicated by opening the movable door, and the insects bred in the first chamber can be released into the second chamber. Therefore, the experiment device provided by the embodiment mainly puts in the pre-emergence larvae or eggs, has the function of a hatching chamber, on the one hand, can reduce physical damage (does not need to use a mosquito suction device in the whole process, will not damage the insects, will not affect the activity of the insects, and improves the accuracy of experimental results), on the other hand, contactless release of adult mosquitoes can reduce mosquito escape and reduce the probability of mosquito bites on the experiment personnel.

[0031] Further, the embodiment provides an insect olfactory behavior experiment device, wherein the first chamber of the embodiment is used for adult emergence and preferably is also used for larva hatching. Here, the larvae and adult insects are directly placed in the first chamber for breeding, for mosquito olfactory behavior experiment. Moreover, in the process of transferring the larvae and adult insects into the first chamber, a mosquito suction device is not needed, and the larvae and adult insects will not be damaged.

[0032] Further, the embodiment provides an insect olfactory behavior experiment device. The first chamber is provided with a temperature control device, a humidity control device, a temperature detection device and a humidity detection device. In addition, the chamber door of the first chamber is provided as a mesh door to enable the air in the first chamber to circulate with the outside air. Here, the above-mentioned arrangement can provide a suitable environment for breeding insects in the first chamber. Further, the movable door is designed as a mesh breathable door, so that the internal environment of the second chamber is consistent with that of the first chamber, thereby facilitating the survival of insects. In addition, the movable door is designed as an upward lifting door, so that the experimenter only needs to lift upward to release the insects in the first chamber into the second chamber, and the experimenter will not be in direct contact with the insects during the whole process. In summary, by arranging the temperature and room temperature control devices and detection devices in the first chamber, and designing the movable door between the second chamber and the first chamber as a mesh breathable door, the temperature and humidity in the first chamber and the second chamber can be controlled and adjusted, thereby further improving the accuracy of the experiment.

[0033] Further, the embodiment provides an insect olfactory behavior experiment device. The first chamber is provided with a temperature control device, a humidity control device, a temperature detection device and a humidity detection device. In addition, the chamber door of the first chamber is provided as a mesh door to enable the air in the first chamber to circulate with the outside air. Here, the above-mentioned arrangement can provide a suitable environment for breeding insects in the first chamber. Further, the movable door is designed as a mesh breathable door, so that the internal environment of the second chamber is consistent with that of the first chamber, thereby facilitating the survival of insects. In addition, the movable door is designed as an upward lifting door, so that the experimenter only needs to lift upward to release the insects in the first chamber into the second chamber, and the experimenter will not be in direct contact with the insects during the whole process. In summary, by arranging the temperature and room temperature control devices and detection devices in the first chamber, and designing the movable door between the second chamber and the first chamber as a mesh breathable door, the temperature and humidity in the first chamber and the second chamber can be controlled and adjusted, thereby further improving the accuracy of the experiment.

[0034] Further, the embodiment provides an insect olfactory behavior experiment device. The first chamber is provided with a temperature control device, a humidity control device, a temperature detection device and a humidity detection device. In addition, the chamber door of the first chamber is provided as a mesh door to enable the air in the first chamber to circulate with the outside air. Here, the above-mentioned arrangement can provide a suitable environment for breeding insects in the first chamber. Further, the movable door is designed as a mesh breathable door, so that the internal environment of the second chamber is consistent with that of the first chamber, thereby facilitating the survival of insects. In addition, the movable door is designed as an upward lifting door, so that the experimenter only needs to lift upward to release the insects in the first chamber into the second chamber, and the experimenter will not be in direct contact with the insects during the whole process. In summary, by arranging the temperature and room temperature control devices and detection devices in the first chamber, and designing the movable door between the second chamber and the first chamber as a mesh breathable door, the temperature and humidity in the first chamber and the second chamber can be controlled and adjusted, thereby further improving the accuracy of the experiment.

[0035] Further, the embodiment provides an insect olfactory behavior experiment device. The first chamber is provided with a temperature control device, a humidity control device, a temperature detection device and a humidity detection device. In addition, the chamber door of the first chamber is provided as a mesh door to enable the air in the first chamber to circulate with the outside air. Here, the above-mentioned arrangement can provide a suitable environment for breeding insects in the first chamber. Further, the movable door is designed as a mesh breathable door, so that the internal environment of the second chamber is consistent with that of the first chamber, thereby facilitating the survival of insects. In addition, the movable door is designed as an upward lifting door, so that the experimenter only needs to lift upward to release the insects in the first chamber into the second chamber, and the experimenter will not be in direct contact with the insects during the whole process. In summary, by arranging the temperature and room temperature control devices and detection devices in the first chamber, and designing the movable door between the second chamber and the first chamber as a mesh breathable door, the temperature and humidity in the first chamber and the second chamber can be controlled and adjusted, thereby further improving the accuracy of the experiment.

[0036] In conclusion, the insect olfactory behavior experiment device provided in the embodiment integrates an olfactometer and a wind tunnel, and can simultaneously realize integration of wind tunnel testing and selection behavior testing.

[0037] The above description is only a summary of the technical scheme of the present application. In order to more clearly understand the technical means of the present application, and can be implemented in accordance with the content of the specification, the following is a detailed description of the preferred embodiments of the present application with reference to the accompanying drawings. BRIEF DESCRIPTION OF DRAWINGS

[0038] Figure 1 is a structural schematic diagram of an insect olfactory behavior experiment device provided by an embodiment of the present application.

[0039] Figure 2 is an experimental result diagram of experimental embodiment 1 of the present application; wherein, Figure 2 *** in the above table indicates significant difference, and the P value is less than 0.001. DETAILED DESCRIPTION

[0040] In order to further illustrate the technical means and effects adopted by the present application to achieve the predetermined purpose, the following will be described in detail with reference to the preferred embodiments and the accompanying drawings. In the following description, different "an embodiment" or "embodiments" do not necessarily refer to the same embodiment. In addition, the specific features, structures, or characteristics in one or more embodiments can be combined in any suitable form.

[0041] Embodiment 1

[0042] The present embodiment provides an insect olfactory behavior experiment device, wherein, as shown in Figure 1 the insect olfactory behavior experiment device of the present embodiment includes a first chamber 1 and an experimental chamber. The first chamber 1 is used for breeding insects. The experimental chamber is used for insect olfactory behavior experiment; wherein, the experimental chamber includes a second chamber 2. The second chamber 2 is used to provide free activity space for insects for insect olfactory behavior experiment. The first chamber 1 and the second chamber 2 are separated by a movable door 12; wherein, by opening the movable door 12, the first chamber 1 and the second chamber 2 are communicated, so as to release the insects bred in the first chamber 1 into the second chamber 2.

[0043] This embodiment provides an experimental device for insect olfactory behavior. It consists of a first chamber 1 for raising insects and an experimental chamber for conducting experiments on insect olfactory behavior. The first chamber 1 and a second chamber 2 within the experimental chamber are separated by a movable door 12. During the experiment, simply opening the movable door connects the first chamber 1 and the second chamber 2, allowing the insects raised in the first chamber 1 to be released into the second chamber 2. Therefore, this experimental device primarily accommodates pre-emergence larvae or eggs, functioning as an incubation chamber. This reduces physical damage (the entire process eliminates the need for mosquito aspirators, preventing insect damage and affecting their vitality, thus improving the accuracy of experimental results). Furthermore, the contactless release of adult mosquitoes reduces mosquito escape and lowers the chance of researchers being bitten.

[0044] Preferably, in this embodiment, the length, width, and height of the first chamber 1 are all 40cm. The length, width, and height of the second chamber 2 are 90cm, 40cm, and 40cm.

[0045] Preferably, the first chamber 1 and the second chamber 2 are made of glass, plastic or transparent material.

[0046] Preferably, a timer is provided on the outer side (e.g., the outer wall) of the first chamber 1 to count the time of insect larvae hatching and adult emergence.

[0047] Preferably, a timer is provided on the outer side (e.g., the outer wall) of the second chamber 1 to record the time of the insect flight behavior experiment.

[0048] Example 2

[0049] Preferably, this embodiment provides an experimental apparatus for insect olfactory behavior, wherein, as Figure 1 As shown, this embodiment is further designed as follows:

[0050] The first chamber 1 is used for adult emergence, and preferably also for larval hatching. Here, the eggs and larvae are raised directly in the first chamber 1 for mosquito olfactory behavior experiments. Furthermore, no mosquito aspirator is needed during the transfer of the eggs and larvae into the first chamber 1, thus avoiding damage to the eggs and larvae.

[0051] Preferably, the first chamber 1 is equipped with a temperature control device, a humidity control device, a temperature detection device, and a humidity detection device to provide a suitable temperature and humidity environment for raising insects in the first chamber 1.

[0052] Preferably, the first chamber 1 is provided with a chamber door 11 for opening the first chamber 1. The chamber door 11 is a mesh door for allowing air circulation between the first chamber 1 and the outside; preferably, the mesh size of the chamber door 1 is 16-60 mesh, preferably 20 mesh.

[0053] Embodiment 3

[0054] Preferably, the present embodiment provides an insect olfactory behavior experimental device, as shown in the figure, the present embodiment further designs the movable door as follows: Figure 1

[0055] The movable door 12 of the present embodiment is a mesh air-permeable door; wherein the mesh of the movable door 12 is 16-60 mesh, preferably 20 mesh. Here, by designing the movable door 12 as a mesh air-permeable door, the internal environment of the second chamber 2 is consistent with that of the first chamber 1, thereby facilitating the survival of insects.

[0056] Preferably, the movable door 12 is an upward-pulling door, wherein the first chamber 1 and the second chamber 2 are communicated by pulling the movable door 12 upward. Here, the movable door 12 is designed as an upward-pulling door, so that the experimenter only needs to pull upward to release the insects in the first chamber 11 into the second chamber 12, and the experimenter will not be in contact with the insects during the whole process.

[0057] Embodiment 4

[0058] Preferably, the present embodiment provides an insect olfactory behavior experimental device, as shown in the figure, the present embodiment further designs the experimental chamber as follows: Figure 1

[0059] The experimental chamber further comprises: a plurality of test chambers 3, a ventilation device. Wherein each test chamber 3 is in communication with the second chamber 2; wherein the test chamber 3 comprises at least one first test chamber for placing an experimental sample (preferably, the test chamber 3 comprises a plurality of first test chambers for placing different experimental samples), at least one second test chamber for placing a control sample. The ventilation device comprises a ventilation pipeline 5; wherein the ventilation pipeline 5 is connected with the test chamber 3, for ventilating gas into the test chamber 3.

[0060] ​​Preferably, a trap device 4 is arranged in the test cavity 3 to prevent insects entering the test cavity 3 from returning to the second cavity 2; preferably, the trap device 4 is a funnel-shaped structure; the large end of the funnel-shaped structure is arranged close to the second cavity 1, and the small end is arranged away from the second cavity 1 (the large end makes it easy for insects to enter, and the small end is arranged to make it difficult for insects entering the test cavity 3 to return to the second cavity 2). Preferably, the diameter of the large end of the funnel-shaped structure is 10 cm, the diameter of the small end is 2-5 cm, and the length is 8-15 cm. Preferably, the large end of the funnel-shaped structure is located at the entrance end of the test cavity 3; preferably, a certain space is left between the small end of the funnel-shaped structure and the air inlet end of the test cavity 3 to place experimental samples or control samples and accommodate insects (insects selected to enter the test cavity 3); wherein the entrance end and the air inlet end of the test cavity 3 are two ends arranged opposite to each other.

[0061] Preferably, the number of ventilation devices is consistent with the number of test cavities 3, and each ventilation device corresponds to one test cavity 3. The ventilation device further comprises a gas generating device 9, a purification device 7, and a humidifying device 6; wherein the gas generating device 9, the purification device 7, the humidifying device 6, the ventilation pipeline 5, and the test cavity 2 are sequentially connected. Preferably, the ventilation device further comprises a flow meter 8; preferably, the flow meter 8 is connected to the pipeline between the gas generating device 9 and the purification device 7. The purification device 7 comprises an activated carbon purification device. The humidifying device 6 is a distilled water humidifying device.

[0062] Preferably, the movable door 12 is located at the first side of the second cavity 2, and the test cavity 3 is connected to the second side of the second cavity 2 (preferably, the second side of the second cavity 2 is provided with a fixed door). Wherein the first side and the second side are arranged opposite to each other. Preferably, a plurality of mounting holes (preferably circular holes) are arranged on the second side of the second cavity 2, and the test cavity is sealingly connected to the mounting holes. Preferably, the number of mounting holes is consistent with the number of test cavities, and each mounting hole corresponds to one test cavity.

[0063] Preferably, the test cavity is a cylindrical cavity, and the size of the outer peripheral wall of the test cavity is adapted to the size of the mounting hole. Preferably, the diameter of the entrance of the test cavity is about 10 cm.

[0064] The embodiment provides an insect olfactory behavior experiment device. A plurality of test cavities 3 are designed to be connected to the second cavity 2 to place experimental samples or controls, and clean gas is introduced into the test cavities 3 through the ventilation device, so that the corresponding test cavities 3 form test odors and the corresponding test cavities 3 form control odors, on the one hand, and on the other hand, due to the principle of insects flying against the wind, insects in the second cavity 2 will fly against the wind to the test cavities 3, and will select the test cavities with the odors they like according to the types of the odors, thereby performing the olfactory behavior experiment.

[0065] Preferably, the insect olfactory behavior experiment device further comprises a monitoring device, the monitoring device comprising a first monitoring device 10 and a second monitoring device 11; wherein the first monitoring device 10 is used to record the flight behavior of the insects in the second chamber 2, and the second monitoring device 11 is used to record the selection behavior of the insects in the second chamber 2, so as to form video data for data analysis.

[0066] Embodiment 5

[0067] In another aspect, the embodiment provides an insect olfactory behavior experiment method, wherein the insect olfactory behavior experiment is performed by using the insect olfactory behavior experiment device according to any one of the above-mentioned embodiments.

[0068] Preferably, the insect olfactory behavior experiment method comprises the following steps:

[0069] The step of releasing the insects: releasing the insects bred in the first chamber 1 into the second chamber 2 by opening the movable door 12.

[0070] Preferably, in this step: Figure 1 As shown, the insects bred in the first chamber 1 are released into the second chamber 2 by opening the movable door 12 and using wind power or attracting light. Preferably, the wind power is provided by ventilating the first mesh door 11; preferably, the attracting light is arranged in the second chamber 2. Preferably, the time of releasing the insects is recorded by using a timer.

[0071] The step of performing the behavior experiment: placing the experimental sample and the control sample in different test chambers respectively, and opening the ventilation device communicating with the test chamber to make the airflow enter the test chamber.

[0072] The recording step: recording the flight behavior of the insects in the second chamber 2 by using the first monitoring device 10, recording the selection behavior of the insects in the second chamber 2 by using the second monitoring device 11, and counting the number of insects in each test chamber.

[0073] The data analysis step: (1) counting the number of mosquitoes making selection in each arm, and performing significant difference analysis by using Excel, R or the like software; (2) copying the flight video recorded by the camera equipment, analyzing the flight trajectory of the insects to different odors by using video data processing software, and exploring the olfactory guiding mechanism of the insects and the recognition and reaction ability of the insects to chemical signals.

[0074] It should be noted that the "significant difference analysis" mainly involves statistical analysis of the preference difference of the insects to odors under different conditions, so as to determine whether the influence of different odor sources on the behavior of the insects has statistical significance. The significant difference analysis usually adopts statistical methods such as t test or ANOVA (analysis of variance) to compare whether the difference between different treatment groups has statistical significance.

[0075] The "chemical signal" herein refers to: the odor released by the test sample, which is a chemical signal affecting the olfactory behavior of insects.

[0076] Experimental Example 1

[0077] The insect olfactory behavior experiment in this experimental example was carried out by using the above-mentioned insect olfactory behavior experiment device (as shown in Figure 1 The main steps are as follows:

[0078] (I) Mosquito breeding and female mosquito selection

[0079] 1. Open the chamber door 11, and adjust the parameters of the temperature control device and the humidity control device to make the temperature in the first chamber 1 be 28±1℃ and the humidity be 75±5%. Put the mosquito breeding container into the first chamber 1, take a beaker or a water basin as the breeding container, add chlorine-free water, and place the prepared mosquito egg paper or mosquito larvae into the breeding container (the breeding container can be placed in the insect breeding cage or can be placed in the first chamber 1 alone).

[0080] 2. Start the timer and record the hatching time of the larvae or the eclosion time of the adult insects.

[0081] (II) Mosquito selection behavior experiment

[0082] 1. Open the insect breeding cage, or directly put the adult insects into the first chamber 1.

[0083] 2. Open the movable door 12, ventilate from the chamber door 11, and release the adult insects (mosquitoes) into the second chamber 2 by using the wind force to make them fly freely in the chamber; or give an attractive light at the second side of the second chamber 2 to attract the adult insects (mosquitoes) from the first chamber 1 to the second chamber 2. Start the timer and record the release time of the adult insects.

[0084] 3. Put the experimental sample (the components of the experimental sample include plant essential oil and solvent; the solvent is selected to be anhydrous ethanol with a concentration of 10%) and the control sample (anhydrous ethanol with a concentration of 10%) into the test cavities 3 (the experimental sample is placed in the first test cavity and the control sample is placed in the second test cavity) respectively. Open the air generating device 9 to make the air flow into the test cavities 3 at a steady flow rate; open the monitoring device to record the behavior of the insects.

[0085] 4. After a certain period of time, observe and record the number of insects in the test cavities, and statistically analyze the influence of the experimental sample on the behavior of the insects.

[0086] 5. Data statistical analysis.

[0087] (1) Count the number of mosquitoes that make a selection in each test cavity, and perform significant difference analysis by using Excel, R, etc.

[0088] (2) Copy the flight video recorded by the camera equipment, and analyze the flight trajectory of insects in response to different odors through video data processing software to explore the olfactory guidance mechanism of insects and their ability to recognize and respond to chemical signals.

[0089] 6. Analysis of Experimental Data Results

[0090] The above steps were used to conduct bioassays on the attraction of adult mosquitoes. Compared with the solvent control group, the number of mosquitoes in the test chamber of the treatment group (i.e., the test chamber where the experimental sample was placed) was significantly higher than that in the control group (see [link to study]). Figure 2 (As shown). The chi-square test result is X. 2 = 22.302, df = 1, p-value < 0.001. This result shows that the insect olfactory behavior experimental apparatus of this embodiment can be effectively used for insect behavior experiments.

[0091] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any way. Any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present utility model shall still fall within the scope of the technical solution of the present utility model.

Claims

1. An apparatus for insect olfactory behavioral experiments, characterized by, The insect olfactory behavior experiment device comprises: a first chamber for breeding insects; an experiment chamber for conducting insect olfactory behavior experiments; wherein the experiment chamber comprises a second chamber for providing free activity space for insects for insect olfactory behavior experiments; wherein the first chamber and the second chamber are separated by a movable door; wherein the first chamber and the second chamber are communicated by opening the movable door to release the insects bred in the first chamber into the second chamber.

2. The insect olfactory behavioral experiment apparatus according to claim 1, characterized by, The first chamber is used for adult emergence.

3. The insect olfactory behavioral experiment device according to claim 2, characterized in that, The first chamber is also used for larva hatching.

4. The insect olfactory behavioral experiment apparatus according to claim 1, characterized by, The first chamber is provided with temperature control devices, humidity control devices, temperature detection devices and humidity detection devices to provide a temperature and humidity environment suitable for breeding insects in the first chamber; and / or A timer is installed on the outside of the first chamber to record the time of larva hatching and / or adult emergence. And / or A timer is installed on the outside of the second chamber to record the release time of releasing the insects bred in the first chamber into the second chamber.

5. The insect olfactory behavioral experiment device according to claim 1, wherein The first chamber is provided with a chamber door for opening the first chamber.

6. The insect olfactory behavioral experiment device according to claim 5, wherein The chamber door is a mesh door for air circulation between the first chamber and the outside.

7. The device for insect olfactory behavioral experiments according to claim 6, characterized in that, The mesh of the chamber door is 16-60 mesh.

8. The insect olfactory behavioral experiment apparatus according to claim 1, wherein The movable door is a mesh air door; wherein the mesh of the movable door is 16-60 mesh.

9. The insect olfactory behavioral experiment apparatus according to claim 1, wherein The movable door is an upward lifting door, wherein the movable door is moved upward by lifting upward to communicate the first chamber and the second chamber.

10. The device for insect olfactory behavioral experiment according to claim 1, characterized in that, The experiment chamber further comprises: a plurality of test chambers, each of which is in communication with the second chamber; wherein the test chambers comprise at least one first test chamber for placing experimental samples and at least one second test chamber for placing control samples; an air supply device comprising an air supply pipeline; wherein the air supply pipeline is connected with the test chambers for supplying gas into the test chambers.

11. The device for insect olfactory behavioral experiments according to claim 10, wherein The test chamber comprises a plurality of first test chambers for placing different experimental samples.

12. The device for insect olfactory behavioral experiments according to claim 10, wherein A trap device is provided in the test chamber to prevent insects entering the test chamber from returning to the second chamber.

13. The device for insect olfactory behavioral experiments according to claim 12, characterized in that, The trap device is a funnel mesh structure.

14. The device for insect olfactory behavioral experiments according to claim 13, wherein The large end of the funnel mesh structure is close to the second cavity, and the small end is away from the second cavity.

15. The device for insect olfactory behavioral experiments according to claim 14, characterized in that, The large end of the funnel mesh structure is located at the inlet end of the test chamber.

16. The device for insect olfactory behavioral experiments according to claim 14, wherein A set space is left between the small end of the funnel mesh structure and the gas inlet end of the test chamber to place experimental samples or control samples and accommodate insects; wherein the inlet end and the gas inlet end of the test chamber are oppositely arranged.

17. The device for insect olfactory behavioral experiments according to claim 10, wherein The number of the air supply device is consistent with the number of the test chambers.

18. The device for insect olfactory behavioral experiments according to claim 10, wherein, The air supply device further comprises a gas generating device, a purification device and a humidifying device; wherein the gas generating device, the purification device, the humidifying device, the air supply pipeline and the test chamber are sequentially communicated.

19. The insect olfactory behavior experiment device according to claim 18, wherein The purification device comprises an activated carbon purification device.

20. The device of claim 18, wherein, The ventilation device further comprises a flow meter; wherein the flow meter is connected to the pipeline between the gas generating device and the purification device.

21. The device for insect olfactory behavioral experiments according to claim 10, wherein, The movable door is located at a first side of the second chamber, and the test cavities are connected to a second side of the second chamber.

22. The device of claim 21, wherein, The first side and the second side are oppositely arranged.

23. The device of claim 22, wherein, A plurality of mounting holes are formed on the second side of the second chamber; the number of the test cavities is consistent with and one-to-one corresponds to the number of the mounting holes, and the test cavities are mounted at the mounting holes corresponding thereto.

24. The device of any one of claims 1-23, wherein, The insect olfactory behavior experiment device further comprises: A monitoring device, the monitoring device comprises a first monitoring device and a second monitoring device; wherein the first monitoring device is used for recording the flight behavior of the insects in the second chamber, and the second monitoring device is used for recording the selection behavior of the insects in the second chamber, so as to form video data for data analysis.

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