Sample pretreatment micro-fluidic chip for knee joint injury gene detection
By designing an integrated microfluidic chip, the problem of long sample preprocessing time for knee joint gene testing was solved, achieving rapid and efficient sample preprocessing and reducing equipment requirements and operational complexity.
Patent Information
- Application Number
- CN202422856469.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-22
- Publication Date
- 2025-12-16
- Estimated Expiration
- 2034-11-22
AI Technical Summary
In existing technologies, the sample preprocessing process for knee joint gene testing is time-consuming, requires a lot of instruments and equipment, and is quite bulky.
A microfluidic chip for sample pretreatment for knee joint injury gene detection was designed, including a plastic substrate and microchannels, integrating a sample injection slot, mixing chamber, reaction chamber and reagent storage chamber, which are connected by capillary channels, siphon channels and connecting channels to achieve integrated and efficient sample pretreatment.
It improves the speed of sample preprocessing, reduces the requirements for equipment size and quantity, enhances the efficiency and flexibility of sample preprocessing, and reduces the need for manual operation.
Smart Images
Figure CN223674643U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] Embodiments of the present disclosure relate to the field of microfluidic chips, and in particular to a sample pretreatment microfluidic chip for knee injury gene detection. BACKGROUND
[0002] Knee sports injury is a common sports injury, mainly including meniscus injury, anterior cruciate ligament injury, patellar dislocation and cartilage injury, etc. The occurrence of knee sports injury is related to many factors, among which the genetic factor is an important influencing factor. Therefore, through gene detection, the susceptible population of knee sports injury can be screened and preventive strategies can be developed in advance, or personalized treatment and rehabilitation programs can be developed for patients who have already suffered injuries. At present, the main method of gene detection is to use the polymerase chain reaction (PCR) technology, and the sample pretreatment of PCR usually needs to separate cells, tissues and DNA / RNA biological molecules in the sample through oscillators, microcentrifuges, pipettors and other instruments.
[0003] However, when the above-mentioned equipment is used for gene detection of the knee joint, the following technical problems often exist:
[0004] The sample pretreatment stage of the conventional PCR gene detection technology is time-consuming and needs more instrument equipment, and the volume is large.
[0005] The above information disclosed in the background section is only intended to enhance the understanding of the background of the present disclosure concept, and therefore, it can contain information that does not form the prior art known to those of ordinary skill in the art in the country. SUMMARY
[0006] The summary section of the present disclosure is used to introduce the concepts in a brief form, which will be described in detail in the specific embodiments section. The summary section of the present disclosure is not intended to identify key or essential features of the claimed technical solutions, nor is it intended to limit the scope of the claimed technical solutions.
[0007] Some embodiments of the present disclosure propose a sample pretreatment microfluidic chip for knee injury gene detection to solve one or more of the technical problems mentioned in the background section.
[0008] Some embodiments of the present disclosure provide a sample pretreatment microfluidic chip for knee injury gene detection, characterized in that the sample pretreatment microfluidic chip for knee injury gene detection comprises a plastic substrate and a microfluidic channel, wherein the plastic substrate is provided with a sample injection groove, a mixing chamber, a reaction chamber and a reagent storage chamber; the mixing chamber comprises a first mixing chamber and a second mixing chamber, and the reagent storage chamber comprises a first reagent storage chamber, a second reagent storage chamber and a third reagent storage chamber; the microfluidic channel comprises a capillary flow channel, a siphon flow channel and a communication flow channel; the sample injection groove and the first mixing chamber are connected by the capillary flow channel, the first mixing chamber and the reaction chamber are connected by the siphon flow channel, the second mixing chamber and the reaction chamber are connected by the siphon flow channel, the first reagent storage chamber and the first mixing chamber are connected by the communication flow channel, the second reagent storage chamber and the second mixing chamber are connected by the communication flow channel, and the third reagent storage chamber and the second mixing chamber are connected by the communication flow channel.
[0009] Optionally, the reaction chamber comprises a first buffer zone, and the first buffer zone is arranged at one end of the reaction chamber.
[0010] Optionally, the reaction chamber comprises at least one sediment collection zone, and the sediment collection zone is arranged at one side of the reaction chamber.
[0011] Optionally, the reaction chamber further comprises a second buffer zone, one side of the second buffer zone is connected with the first buffer zone by the siphon flow channel, and the other side of the second buffer zone is connected with the outside by the capillary flow channel.
[0012] Optionally, the first reagent storage chamber, the first mixing chamber and the reaction chamber each comprise at least one mixing column.
[0013] Optionally, the sample pretreatment microfluidic chip for knee injury gene detection further comprises an exhaust flow channel, and the first mixing chamber is provided with the exhaust flow channel on the upper side, and the second mixing chamber and the reaction chamber are provided with the exhaust flow channel.
[0014] Optionally, at least one diaphragm is arranged in the siphon flow channel between the first buffer zone and the second buffer zone.
[0015] Optionally, at least one valve is arranged in the siphon flow channel between the first mixing chamber and the reaction chamber, and at least one valve is arranged in the siphon flow channel between the second mixing chamber and the reaction chamber.
[0016] Optionally, the microfluidic channel and the exhaust flow channel are in the same plane, and the microfluidic channel and the exhaust flow channel do not cross each other.
[0017] Optionally, the sample injection groove, the mixing chamber, the reaction chamber, the reagent storage chamber, the first buffer zone and the second buffer zone are distributed in a diffusion shape with a preset center position, the sample injection groove and the first reagent storage chamber are located in the innermost circumferential area, the first mixing chamber, the second reagent storage chamber, the third reagent storage chamber and the second mixing chamber are located in the intermediate circumferential area, and the reaction chamber, the first buffer zone and the second buffer zone are located in the outermost circumferential area. In the use state, the sample pretreatment microfluidic chip for knee injury gene detection is installed on the stage, and when the stage is running, the sample pretreatment microfluidic chip for knee injury gene detection is driven to rotate clockwise or counterclockwise based on the preset center position.
[0018] Some embodiments of the present disclosure provide a sample pretreatment microfluidic chip for knee injury gene detection, which can improve the sample pretreatment speed and reduce the requirements for the size and number of equipment. Specifically, the reason for the low efficiency of most sample pretreatment is that the sample pretreatment steps are more and the operation requirements are higher, and most of them are processed manually. In addition, a large amount of samples is usually used, which results in low sample pretreatment speed and high requirements for equipment and operation. Based on this, some embodiments of the present disclosure provide a sample pretreatment microfluidic chip for knee injury gene detection. The sample pretreatment microfluidic chip for knee injury gene detection includes a plastic substrate and a microfluidic channel. The plastic substrate is provided with a sample injection groove, a mixing chamber, a reaction chamber and a reagent storage chamber. The mixing chamber includes a first mixing chamber and a second mixing chamber, and the reagent storage chamber includes a first reagent storage chamber, a second reagent storage chamber and a third reagent storage chamber. The microfluidic channel includes a capillary flow channel, a siphon flow channel and a communication flow channel. The sample injection groove and the first mixing chamber are connected by the capillary flow channel, the first mixing chamber and the reaction chamber are connected by the siphon flow channel, the second mixing chamber and the reaction chamber are connected by the siphon flow channel, the first reagent storage chamber and the first mixing chamber are connected by the communication flow channel, the second reagent storage chamber and the second mixing chamber are connected by the communication flow channel, and the third reagent storage chamber and the second mixing chamber are connected by the communication flow channel. On the one hand, the chambers required for sample pretreatment related steps are integrated on the plastic substrate, and all related steps are performed on the plastic substrate, which reduces the requirements for the size and number of equipment. On the other hand, the sample pretreatment microfluidic chip for knee injury gene detection requires a small amount of sample, and in the microscale, the effect of surface force will be significantly amplified, which can effectively improve the efficiency of material mixing and reaction, and realize rapid and efficient separation and analysis. Thus, the sample pretreatment speed can be improved, and the requirements for the size and number of equipment can be reduced. BRIEF DESCRIPTION OF DRAWINGS
[0019] The above and other features, advantages, and aspects of embodiments of the present disclosure will become more apparent by describing in detail some embodiments thereof with reference to the annexed drawings in which: like reference numerals refer to like elements throughout. The annexed drawings are intended for purposes of illustration only and shall not limit the scope of the disclosure. The drawings are not necessarily drawn to scale, and certain components, elements, and / or structures that can be shown in the drawings can not be to scale.
[0020] Figure 1 is a structural schematic diagram of a hollow cavity structure and a microfluidic channel in a sample pretreatment microfluidic chip for knee movement injury gene detection of some embodiments of the present disclosure.
[0021] Figure 2 is a combined structural diagram of multiple sample pretreatment microfluidic chips for knee movement injury gene detection of some embodiments of the present disclosure.
[0022] Figure 3 is a structural schematic diagram of a sample pretreatment microfluidic chip for knee movement injury gene detection of some embodiments of the present disclosure after installation.
[0023] Figure 4 is a partial modular example diagram of a sample pretreatment microfluidic chip for knee movement injury gene detection of some embodiments of the present disclosure. DETAILED DESCRIPTION
[0024] Embodiments of the present disclosure will be described in more detail with reference to the drawings. Although certain embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure can be embodied in various forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that the present disclosure will be more thoroughly and completely understood. It should be understood that the drawings of the present disclosure are only for illustrative purposes and should not be construed as limiting the scope of protection of the present disclosure.
[0025] It should also be noted that only parts related to the present application are shown in the drawings for the convenience of description. The embodiments in the present disclosure and the features in the embodiments can be combined with each other without conflict.
[0026] It should be noted that the concepts of "first", "second", etc. mentioned in the present disclosure are only used to distinguish different devices, modules or units, and are not used to limit the order or interdependence of the functions performed by these devices, modules or units.
[0027] It should be noted that the modification of "one" or "multiple" in the present disclosure is illustrative and not restrictive, and those skilled in the art should understand that unless otherwise explicitly indicated in the context, it should be understood as "one or more".
[0028] Names of messages or information exchanged between multiple devices in the embodiments of the present disclosure are only for illustrative purposes, and are not intended to limit the scope of the messages or information.
[0029] The present disclosure will be described in detail below with reference to the accompanying drawings and in conjunction with embodiments.
[0030] Figure 1 FIG. 1 is a structural schematic diagram of a cavity structure and a microfluidic channel in a sample pretreatment microfluidic chip for knee sports injury gene detection according to some embodiments of the present disclosure. Figure 1 The sample pretreatment microfluidic chip includes a sample injection groove 1, a capillary flow channel 2, a first mixing chamber 3, a mixing column 4, a reaction chamber 5, a siphon flow channel 6, a sediment collection area 7, a mixing column 8, a first reagent storage chamber 9, a mixing column 10, a second reagent storage chamber 11, a third reagent storage chamber 12, a second mixing chamber 13, a siphon flow channel 14, a siphon flow channel 15, a second buffer area 16, a first buffer area 17, and a capillary flow channel 18.
[0031] Figure 2 FIG. 2 is a combined structural diagram of multiple sample pretreatment microfluidic chips for knee sports injury gene detection according to some embodiments of the present disclosure. Figure 2 The sample pretreatment microfluidic chip includes a plastic substrate 201.
[0032] Figure 3 FIG. 3 is a structural schematic diagram of the sample pretreatment microfluidic chip for knee sports injury gene detection after installation according to some embodiments of the present disclosure. Figure 3 The sample pretreatment microfluidic chip includes a carrier 301 and a plastic substrate 201.
[0033] In some embodiments, the sample pretreatment microfluidic chip for knee injury gene detection can include a plastic substrate 201 and a microfluidic channel. Figures 1-2 In some embodiments, the plastic material of the plastic substrate 201 can include, but is not limited to, polycarbonate (PC), polystyrene (PS), and polypropylene (PP). The microfluidic channel can include a capillary flow channel 2, a siphon flow channel 6, a siphon flow channel 14, a siphon flow channel 15, and a capillary flow channel 18. The plastic substrate 201 can be used to set the sample injection groove 1, the capillary flow channel 2, the first mixing chamber 3, the mixing column 4, the reaction chamber 5, the siphon flow channel 6, the sediment collection area 7, the mixing column 8, the first reagent storage chamber 9, the mixing column 10, the second reagent storage chamber 11, the third reagent storage chamber 12, the second mixing chamber 13, the second siphon flow channel 14, the third siphon flow channel 15, the second buffer area 16, the first buffer area 17, and the second capillary flow channel 18. As shown in FIG. 2, three sets of the same cavity structure and microfluidic channel structure can be provided on one plastic substrate 201. Figure 2
[0034] In some embodiments, the plastic substrate 201 can be provided with a sample injection groove 1, a mixing chamber, a reaction chamber 5, and a reagent storage chamber. The sample injection groove 1 can be used to receive an injected sample reagent. For example, 200 microliters of sample solution can be injected into the sample injection groove 1. The reagent storage chamber can be used to receive and store sample pretreatment reagents. The mixing chamber can be used to mix sample solution and sample pretreatment solution. The reaction chamber 5 can be used to receive the mixed solution from the mixing chamber for further reaction. The sample injection groove 1, the mixing chamber, the reaction chamber 5, and the reagent storage chamber can all be made of the same material as the plastic substrate 201.
[0035] In some embodiments, the mixing chamber can include a first mixing chamber 3 and a second mixing chamber 13. The reagent storage chamber can include a first reagent storage chamber 9, a second reagent storage chamber 11, and a third reagent storage chamber 12. The first reagent storage chamber 9 can be used to store a type of solution reagent required for sample pretreatment. The type of solution reagent required for sample pretreatment can include but is not limited to a lysis reagent. The second reagent storage chamber 11 can be used to store a second type of solution reagent required for sample pretreatment. The second type of solution reagent required for sample pretreatment can include but is not limited to a nucleic acid removal solution. The third reagent storage chamber 12 can be used to store a third type of solution reagent required for sample pretreatment. The third type of solution reagent required for sample pretreatment can be a reaction premix solution. The first mixing chamber 3 can be used to mix solutions flowing from the sample injection groove 1 and the first reagent storage chamber 9 through the capillary flow channel 2. The second mixing chamber 13 can be used to mix solutions flowing from the second reagent storage chamber 11 and the third reagent storage chamber 12 through the communication flow channel.
[0036] In some embodiments, the microfluidic channel can include a capillary flow channel, a siphon flow channel, and a communication flow channel. The materials of the capillary flow channel, the siphon flow channel, and the communication flow channel can include but are not limited to polydimethylsiloxane (PDMS), polymethyl methacrylate (PMMA), and glass. The diameters of the capillary flow channel, the siphon flow channel, and the communication flow channel can not be limited and can be adjusted according to experimental requirements. The diameters can range from 0.1 mm to 0.5 mm. The capillary flow channel, the siphon flow channel, and the communication flow channel can be used to connect the mixing chambers, the reagent storage chambers, and the reaction chamber 5. The capillary flow channel can be smaller in size than the communication flow channel. The capillary flow channel can have a stronger speed-limiting effect on the internal solution than the siphon flow channel and the capillary flow channel. The siphon flow channel can be provided with a valve.
[0037] In some embodiments, the sample injection slot 1 is connected to the first mixing chamber 3 by a capillary flow channel 2. The first mixing chamber 3 is connected to the reaction chamber 5 by a siphon flow channel 6. The second mixing chamber 13 is connected to the reaction chamber 5 by a siphon flow channel 14. The first reagent storage chamber 9 is connected to the first mixing chamber 3 by a communication flow channel. The second reagent storage chamber 11 is connected to the second mixing chamber 13 by a communication flow channel. The third reagent storage chamber 12 is connected to the second mixing chamber 13 by a communication flow channel. The capillary flow channel, the siphon flow channel and the communication flow channel can be used to transport solution.
[0038] Optionally, as shown in FIG. 1, the reaction chamber 5 can further include a first buffer zone 17. The first buffer zone 17 can be disposed at one end of the reaction chamber 5. The first buffer zone 17 can be used to slow down the flow rate of liquid and stabilize the flow rate of solution in the microfluidic channel. Figure 1
[0039] Optionally, as shown in FIG. 1, the reaction chamber 5 can include at least one sediment collection zone 7. The sediment collection zone 7 can be disposed at one side of the reaction chamber 5. For example, as shown in FIG. 1, the reaction chamber can have three sediment collection zones 7. The sediment collection zone 7 can be used to receive residue generated by sample pretreatment reaction. Figure 1 Figure 1
[0040] Optionally, as shown in FIG. 1, the reaction chamber 5 can further include a second buffer zone 16. One side of the second buffer zone 16 can be connected to the first buffer zone 17 by a siphon flow channel 15. The other side of the second buffer zone 16 can be connected to the outside by a capillary flow channel 18. The second buffer zone 16 can also be used to slow down the flow rate of liquid and stabilize the flow rate of solution in the microfluidic channel. The capillary flow channel 18 can be used to connect to a centrifuge tube, which can be used to hold solution in the reaction chamber 5. Figure 1
[0041] Optionally, as shown in FIG. 1, the first reagent storage chamber 9, the first mixing chamber 3 and the reaction chamber 5 can each include at least one mixing column. For example, as shown in FIG. 1, the first mixing chamber 3 can have two mixing columns 4. The reaction chamber 5 can have three mixing columns 8. The first reagent storage chamber 9 can have two mixing columns 10. The material of the mixing column is consistent with the first reagent storage chamber 9, the first mixing chamber 3 and the reaction chamber 5, and can be an integrally formed structure. The mixing column can be used to improve the mixing efficiency of solution in the mixing chamber. Figure 1 Figure 1
[0042] Optionally, as shown in FIG. 1, the first reagent storage chamber 9, the first mixing chamber 3 and the reaction chamber 5 can each include at least one mixing column. For example, as shown in FIG. 1, the first mixing chamber 3 can have two mixing columns 4. The reaction chamber 5 can have three mixing columns 8. The first reagent storage chamber 9 can have two mixing columns 10. The material of the mixing column is consistent with the first reagent storage chamber 9, the first mixing chamber 3 and the reaction chamber 5, and can be an integrally formed structure. The mixing column can be used to improve the mixing efficiency of solution in the mixing chamber. Figure 1 As shown in the above, the sample pretreatment microfluidic chip for detecting knee injury gene can further comprise an exhaust flow channel. The exhaust flow channel can be arranged on the upper side of the first mixing chamber 3. The exhaust flow channel can be arranged between the second mixing chamber 13 and the reaction chamber 5. The material of the exhaust flow channel is consistent with the siphon flow channel and the capillary flow channel, and the size is not limited, which can be adjusted according to the experimental requirements. The exhaust flow channel can be used to exhaust the gas in the mixing chamber and balance the air pressure in the chamber.
[0043] Optionally, as shown in the above, Figure 1 The siphon flow channel between the first buffer area 17 and the second buffer area 16 can be provided with at least one diaphragm. The diaphragm can be made of controllable material, which can change its shape or properties according to external stimulation, so as to control the flow and reaction of the liquid in the microfluidic channel. The controllable material can include but is not limited to polyvinyl alcohol (PVA) and polydimethylsiloxane (PDMS). The diaphragm can be a variable film. The diaphragm can be used to finely control the flow of fluid.
[0044] Optionally, as shown in the above, Figure 1 The siphon flow channel between the first mixing chamber 3 and the reaction chamber 5 can comprise at least one valve, and the siphon flow channel between the second mixing chamber 13 and the reaction chamber 5 can comprise at least one valve. The valve can also be made of the controllable material. The valve can be a siphon valve, which can control the flow of fluid in the microfluidic chip. The siphon valve can be used for applications requiring rapid switching of fluid flow state, such as fluid routing control, sample injection, etc.
[0045] Optionally, as shown in the above, Figures 1-2 The microfluidic channel and the exhaust flow channel are in the same horizontal plane, and there is no intersection between the microfluidic channel and the exhaust flow channel. The exhaust flow channel without intersection and in the same plane can reduce the obstruction to the flow of liquid and reduce the possibility of experimental error.
[0046] Optionally, as shown in the above, Figure 3As shown, the sample injection groove, the mixing chamber, the reaction chamber 5, the reagent storage chamber, the first buffer zone 17 and the second buffer zone 16 can be distributed in a spread shape with a preset center position. The sample injection groove and the first reagent storage chamber 9 can be located in the innermost circumferential area. The first mixing chamber 3, the second reagent storage chamber 11, the third reagent storage chamber 12 and the second mixing chamber 13 can be located in the middle circumferential area. The reaction chamber 5, the first buffer zone 17 and the second buffer zone 16 can be located in the outermost circumferential area. In use, the sample pretreatment microfluidic chip for knee injury gene detection can be installed on the carrier 301. The carrier 301 can be a machine that can drive the sample pretreatment microfluidic chip for knee injury gene detection to rotate. For example, as shown in the figure, Figure 3 As shown, the carrier 301 can include a tray part, a motor part connected to the lower end of the tray part, and a tray cover part. The lower end of the tray part can be connected to a motor, and the tray cover part can be installed on the sample pretreatment microfluidic chip for knee injury gene detection. The motor is arranged at the center position and drives the tray part, the sample pretreatment microfluidic chip for knee injury gene detection and the tray cover part to rotate when in operation. It should be noted that, Figure 4 Only the tray part and the sample pretreatment microfluidic chip for knee injury gene detection installed on the tray part are shown. When the carrier 301 is in operation, the carrier 301 drives the sample pretreatment microfluidic chip for knee injury gene detection to rotate clockwise or counterclockwise based on the preset center position. The various components are distributed in different circumferential areas, which can automatically control the flow direction of the liquid according to the different forces during rotation. The rotation speed and direction of the carrier 301 can be adjusted to control the sample pretreatment microfluidic chip for knee injury gene detection, so as to adjust the flow rate and direction of the solution in the sample pretreatment microfluidic chip for knee injury gene detection, complete the whole sample pretreatment process, and obtain the pretreatment result of the separated leukocytes in the sample.
[0047] Optionally, the sample injection groove, the mixing chamber, the reaction chamber and the reagent storage chamber on the plastic substrate 201 can each have a corresponding independent partition, and the independent partitions can be detachably fitted together. The shapes of the various independent partitions are complementary, for example, the protruding part of one of the two adjacent independent partitions can correspond to the recessed part of the other independent partition. Among them, Figure 4 A detached example of the independent partition 401 of the first mixing chamber is shown. It should be noted that, Figure 4 Only an example of the independent partition of one of the chambers is shown, and the details of the other independent partitions are not shown in the figure. The above-mentioned each independent partition can be fitted together through the convex and concave parts on each independent partition. The above-mentioned each independent partition can be a complete plastic substrate in the fitted state. The above-mentioned micro flow channel between the above-mentioned independent partitions can be a flow-through state in the fitted state. The above-mentioned independent partitions can have at least one replaceable component, which can be a combinable structure.
[0048] The above-mentioned optional embodiment, as one of the application points of the embodiments of the present disclosure, solves the technical problem of "high degree of integration and poor flexibility of the sample pretreatment microfluidic chip for knee injury gene detection". The factors leading to high degree of integration and poor flexibility are as follows: each mixing chamber, each reagent storage chamber, the reaction chamber and each buffer zone of the above-mentioned sample pretreatment microfluidic chip for knee injury gene detection are integrated on one plastic substrate, which is not easy to supplement other functional components and replace damaged parts. If the above-mentioned factors are solved, the problem of high degree of integration and poor flexibility can be solved. In order to achieve this effect, the embodiments of the present disclosure divide each functional component of the above-mentioned sample pretreatment microfluidic chip for knee injury gene detection into multiple independent partitions, and the above-mentioned each independent partition can be in a detachable mode. On the one hand, as long as the fitting parts and the flow channel can correspond, each independent partition can realize more flexible combination. On the other hand, each independent partition can also replace the independent partition of the damaged part separately, which reduces the material waste caused by replacing the entire microfluidic chip because of the damage of a part. Thus, without affecting the original function, the degree of integration of the sample pretreatment microfluidic chip for knee injury gene detection is reduced, and the flexibility is improved.
[0049] Some embodiments of the present disclosure provide a sample pretreatment microfluidic chip for knee injury gene detection, which can improve the sample pretreatment speed and reduce the requirements for the size and number of devices. Specifically, the reason for the low efficiency of most sample pretreatment is that the sample pretreatment has many steps and high operation requirements, and is usually processed manually. In addition, a large amount of sample is usually used, which results in low sample pretreatment speed and high requirements for devices and operation. Based on this, some embodiments of the present disclosure provide a sample pretreatment microfluidic chip for knee injury gene detection. The sample pretreatment microfluidic chip for knee injury gene detection includes a plastic substrate and a microfluidic channel. The plastic substrate is provided with a sample injection groove, a mixing chamber, a reaction chamber, and a reagent storage chamber. The mixing chamber includes a first mixing chamber and a second mixing chamber. The reagent storage chamber includes a first reagent storage chamber, a second reagent storage chamber, and a third reagent storage chamber. The microfluidic channel includes a capillary flow channel, a siphon flow channel, and a communication flow channel. The sample injection groove and the first mixing chamber are connected by the capillary flow channel. The first mixing chamber and the reaction chamber are connected by the siphon flow channel. The second mixing chamber and the reaction chamber are connected by the siphon flow channel. The first reagent storage chamber and the first mixing chamber are connected by the communication flow channel. The second reagent storage chamber and the second mixing chamber are connected by the communication flow channel. The third reagent storage chamber and the second mixing chamber are connected by the communication flow channel. In one aspect, the chambers required for sample pretreatment related steps are integrated on the plastic substrate, and all related steps are performed on the plastic substrate, which reduces the requirements for the size and number of devices. In another aspect, the sample pretreatment microfluidic chip for knee injury gene detection requires a small amount of sample, and the surface force is significantly amplified at the microscale, which can effectively improve the efficiency of material mixing and reaction, and realize rapid and efficient separation and analysis. Thus, the sample pretreatment speed can be improved, and the requirements for the size and number of devices can be reduced.
[0050] The above description is merely some preferred embodiments of the present disclosure and a description of the principles of the technology used. Those skilled in the art should understand that the scope of the application involved in the embodiments of the present disclosure is not limited to the technical solutions formed by the specific combinations of the above technical features, and should also cover other technical solutions formed by any combinations of the above technical features or equivalent features without departing from the above inventive concept. For example, the above features are replaced with the technical features disclosed in the embodiments of the present disclosure (but not limited to) having similar functions to form technical solutions.
Claims
1. A sample pretreatment microfluidic chip for knee injury gene detection, characterized in that, The sample pretreatment microfluidic chip for knee injury gene detection comprises a plastic substrate and a microfluidic channel, The plastic substrate is provided with a sample injection groove, a mixing chamber, a reaction chamber and a reagent storage chamber; The mixing chamber comprises a first mixing chamber and a second mixing chamber, and the reagent storage chamber comprises a first reagent storage chamber, a second reagent storage chamber and a third reagent storage chamber; The microfluidic channel comprises a capillary flow channel, a siphon flow channel and a communication flow channel; The sample injection groove and the first mixing chamber are connected by a capillary flow channel, the first mixing chamber and the reaction chamber are connected by a siphon flow channel, the second mixing chamber and the reaction chamber are connected by a siphon flow channel, the first reagent storage chamber and the first mixing chamber are connected by a communication flow channel, the second reagent storage chamber and the second mixing chamber are connected by a communication flow channel, and the third reagent storage chamber and the second mixing chamber are connected by a communication flow channel.
2. The sample pretreatment microfluidic chip for detecting knee injury genes according to claim 1, characterized in that, The reaction chamber comprises a first buffer zone provided at one end of the reaction chamber.
3. The sample pretreatment microfluidic chip for detecting knee injury genes according to claim 2, characterized in that, The reaction chamber comprises at least one sediment collection zone provided at one side of the reaction chamber.
4. The sample pretreatment microfluidic chip for detecting knee injury genes according to claim 3, characterized in that, The reaction chamber further comprises a second buffer zone, one side of the second buffer zone is connected with the first buffer zone through a siphon flow channel, and the other side of the second buffer zone is connected with the outside through a capillary flow channel.
5. The sample pretreatment microfluidic chip for detecting knee injury genes according to claim 4, characterized in that, The first reagent storage chamber, the first mixing chamber and the reaction chamber each comprise at least one mixing column.
6. The sample pretreatment microfluidic chip for detecting knee injury genes according to claim 5, characterized in that, The sample pretreatment microfluidic chip for knee injury gene detection further comprises an exhaust flow channel, the first mixing chamber is provided with an exhaust flow channel on the upper side, and the second mixing chamber and the reaction chamber are provided with an exhaust flow channel.
7. The sample pretreatment microfluidic chip for detecting knee injury genes according to claim 6, characterized in that, At least one diaphragm is arranged in the siphon flow channel between the first buffer zone and the second buffer zone.
8. The sample pretreatment microfluidic chip for detecting knee injury genes according to claim 7, characterized in that, At least one valve is arranged in the siphon flow channel between the first mixing chamber and the reaction chamber, and at least one valve is arranged in the siphon flow channel between the second mixing chamber and the reaction chamber.
9. The sample pretreatment microfluidic chip for detecting knee injury genes according to claim 8, characterized in that, The microfluidic channel and the exhaust flow channel are in the same plane, and do not cross each other.
10. The sample pretreatment microfluidic chip for detecting knee injury genes according to claim 9, characterized in that, The sample injection groove, the mixing chamber, the reaction chamber, the reagent storage chamber, the first buffer zone and the second buffer zone are distributed in a diffusion shape with a preset center position, the sample injection groove and the first reagent storage chamber are located in the innermost circular region, the first mixing chamber, the second reagent storage chamber, the third reagent storage chamber and the second mixing chamber are located in the intermediate circular region, and the reaction chamber, the first buffer zone and the second buffer zone are located in the outermost circular region, in the use state, the sample pretreatment microfluidic chip for knee injury gene detection is installed on a carrier, and the carrier drives the sample pretreatment microfluidic chip for knee injury gene detection to rotate clockwise or counterclockwise based on the preset center position when the carrier operates.