Portable biochemical coagulation and fluorescence immunoassay integrated analysis device

Abstract

The utility model relates to the field of microfluidic detection and fluorescence immunoassay, in particular to a portable biochemical blood coagulation and fluorescence immunoassay integrated analysis device, which comprises a shell, a power supply arranged in the shell, two support plates arranged in the shell, a fluorescent bracket fixedly connected to the upper ends of the support plates, a support arranged in the shell, a lamp source arranged at the upper end of the support, and a light source arranged at the lower end of the lamp source. A microfluidic detection module is mounted in the support, a fluorescence immunodetection module is mounted at the upper end of the fluorescence bracket, an optical module is arranged in the fluorescence immunodetection module, and a bar code scanner for confirming information of a detection chip or a reagent strip is arranged on one side of the optical module; compared with the traditional detection equipment with a single function, the double detection functions of biochemistry and fluorescence immunity are realized, the detection efficiency and comprehensiveness are greatly improved, meanwhile, the kit also supports the simultaneous detection of three reagent strips with different specifications, and the flexibility and convenience of the detection are further enhanced.

Description

TECHNICAL FIELD

[0001] The utility model relates to micro -fluidic detection and fluorescent immunoassay field especially relates to a portable biochemical coagulation and fluorescent immunity integrated analysis device. BACKGROUND

[0002] Micro -fluidic chip can be concentrated on the chip of several square centimeters, carries out sample pretreatment, separation and detection etc., and fluorescent immunoassay technology has the advantages such as strong specificity, high sensitivity, good practicability, therefore it is used to measure the low content biological active compound, for example protein (enzyme, receptor, antibody), hormone (steroid compound, thyroid hormone, phthalic hormone), drug and microorganism etc.

[0003] However, although there are numerous micro -fluidic instrument analyzers and fluorescent immunoassay analyzers on the market at present, most of these instruments can only realize single micro -fluidic detection function or fluorescent immunoassay function, and cannot simultaneously have both micro -fluidic detection and fluorescent immunoassay functions, thus having certain limitations in practicability.

[0004] Therefore, in view of the above-mentioned existing micro -fluidic and fluorescent immunoassay analyzer only having single function, lacking double detection capability of micro -fluidic and fluorescent immunoassay, and leading to limited practicability, a portable biochemical coagulation and fluorescent immunity integrated analysis device can be designed to realize double detection function of biochemical and fluorescent immunoassay, greatly improve detection efficiency and comprehensiveness, and also support simultaneous detection of three different specifications of reagent strips, further enhance the flexibility and convenience of detection. UTILITY MODEL CONTENTS

[0005] In order to overcome the problem that the existing micro -fluidic and fluorescent immunoassay analyzer only has single function, lacks double detection capability of micro -fluidic and fluorescent immunoassay, and leads to limited practicability.

[0006] The technical scheme of the utility model is as follows: a portable biochemical coagulation and fluorescent immunity integrated analysis device, including the casing, the casing is built in the power supply, the casing is provided with two support plates, the upper end of the support plate is fixedly connected with the fluorescent support, the casing is installed with the support, the upper end of the support is installed with the light source, the support is installed with the micro -fluidic detection module, the upper end of the fluorescent support is installed with the fluorescent immunoassay detection module, the fluorescent immunoassay detection module is provided with the optical module, one side of the optical module is provided with the bar code scanner of confirming detection chip or reagent strip information.

[0007] As preferred, the microfluidic detection module comprises a detection chamber, the detection chamber is installed in a support, an access cabin for conveying a detection chip is installed on one side of the detection chamber, a fixing seat is installed in the access cabin, the detection chip is placed on the fixing seat, an NFC chip is attached to the detection chip, an NFC detection structure is installed on the support, and a spectrometer is installed at the lower end of the support.

[0008] As preferred, a magnet is arranged at the upper end of the fixing seat, and an iron sheet is inlaid at the bottom of the magnet, and the detection chip is connected to the fixing seat through the magnet.

[0009] As preferred, the fluorescence immunoassay module comprises a lead screw motor, the lead screw motor is installed on a fluorescence support, a threaded hole is formed in the output shaft of the lead screw motor, and a detection table is connected to the threaded hole.

[0010] As preferred, three detection positions are arranged, which are a single reagent strip position, a double reagent strip position and a triple reagent strip position.

[0011] As preferred, the optical module comprises a box, the upper end of the detection table is provided with the box, an LED light source is installed in the box, and a receiving diode is installed in the box and located on one side of the LED light source.

[0012] The beneficial effects of the utility model are as follows: compared with the traditional single-function detection equipment, the utility model realizes the dual detection function of biochemistry and fluorescence immunoassay, greatly improves the detection efficiency and comprehensiveness, supports the simultaneous detection of three different specifications of reagent strips, further enhances the flexibility and convenience of detection, in addition, the device is also provided with a barcode scanner and an NFC detection structure, can automatically read the information of the detection chip or the reagent strip, simplifies the operation process and reduces the human error. BRIEF DESCRIPTION OF DRAWINGS

[0013] Figure 1 The utility model discloses a portable biochemical coagulation and fluorescence immunoassay integrated analysis device biochemical coagulation detection module and fluorescence detection module three-dimensional structure schematic diagram shows;

[0014] Figure 2 The utility model discloses a portable biochemical coagulation and fluorescence immunoassay integrated analysis device shell three-dimensional structure schematic diagram shows;

[0015] Figure 3 The utility model discloses a portable biochemical coagulation and fluorescence immunoassay integrated analysis device biochemical coagulation detection module access cabin state three-dimensional structure schematic diagram shows;

[0016] Figure 4 The utility model discloses a portable biochemical coagulation and fluorescence immunoassay integrated analysis device biochemical coagulation detection module access cabin state three-dimensional structure schematic diagram shows;

[0017] Figure 5 The utility model discloses a portable biochemical coagulation and fluorescent immunity integrated analysis device immunodetection module single reagent strip three-dimensional structure schematic diagram shows.

[0018] Figure 6 The utility model discloses a portable biochemical coagulation and fluorescent immunity integrated analysis device immunodetection module double reagent strip three-dimensional structure schematic diagram shows.

[0019] Figure 7 The utility model discloses a portable biochemical coagulation and fluorescent immunity integrated analysis device immunodetection module triple reagent strip three-dimensional structure schematic diagram shows.

[0020] Figure 8 The utility model discloses a portable biochemical coagulation and fluorescent immunity integrated analysis device immunodetection module cross section drawing shows.

[0021] The utility model discloses a portable biochemical coagulation and fluorescent immunity integrated analysis device immunodetection module cross section drawing shows. Specific implementation

[0022] The utility model further carries out the explanation with the drawings and examples.

[0023] Please refer to Figures 1-8The utility model provides a kind of embodiment: a portable biochemical coagulation and fluorescence immuno-integrated analysis device, including shell 1, power supply is built-in in shell 1, two pieces of support plate 2 are arranged in shell 1, the upper end of support plate 2 is fixedly connected with fluorescence support 3, support 4 is installed in shell 1, the upper end of support 4 is installed with light source 5, microfluidic detection module is installed in support 4, fluorescence immune detection module is installed in the upper end of fluorescence support 3, optical module is arranged in fluorescence immune detection module, the side of optical module is provided with the bar code scanner 6 of confirmation detection chip 10 or reagent strip information, shell 1 is as the basic structure of entire device, shell 1 plays the role of protection internal component, support plate 2 plays the role of support and fixed fluorescence support 3, fluorescence support 3 is used to support fluorescence immune detection module, so that user can easily carry out fluorescence immune detection operation, microfluidic detection module is used to carry out biochemical coagulation etc.

[0024] Please refer to Figure 1 、 Figure 3 And Figure 6In the embodiment, the microfluidic detection module comprises a detection chamber 7 installed in the support 4, one side of the detection chamber 7 is provided with an access cabin 8 for conveying the detection chip 10, the access cabin 8 is provided with a fixing seat 9, the detection chip 10 is placed on the fixing seat 9, the detection chip 10 is pasted with an NFC chip 11, the support 4 is provided with an NFC detection structure 12, the lower end of the support is provided with a spectrometer 13, the upper end of the fixing seat 9 is provided with a magnet, the bottom of the magnet is inlaid with an iron sheet, the detection chip 10 is connected with the fixing seat 9 through the magnet, the access cabin 8 is fixed on the support 4 and driven to extend and retract through the cooperation of the built-in gear and the rack, in addition, a DC brushless motor is fixed on the access cabin 8, the motor plays a centrifugal role, during detection, the access cabin 8 extends, so that the operator can place the detection chip 10 (the chip can be a biochemical chip or a coagulation chip, both of which can be used in the microfluidic detection module) on the fixing seat 9, at this time, the magnet makes the detection chip 10 and the fixing seat 9 attract together, in the process that the detection chip 10 and the fixing seat 9 attract together, the dilution cup film in the detection chip 10 is automatically torn, the diluent flows into the detection chip 10, at the same time, the fixing seat 9 is provided with a ring gear which can mesh with the teeth on the detection chip 10, so as to realize the circumferential locking of the detection chip 10, when the access cabin 8 is retracted, the detection chip 10 enters the detection chamber 7, the detection chamber 7 is provided with a heating film above and below, a temperature sensor is provided beside, so as to control the temperature in the detection chamber 7, the detection chip 10 is pasted with the NFC chip 11, the NFC detection structure 12 reads the information of the NFC chip 11, after the information reading is completed, the DC brushless motor drives the detection chip 10 to perform centrifugal motion, after the centrifugal motion is completed, the sample and the diluent will flow into the detection holes around the detection chip 10 and react with the freeze-dried balls in the holes, the light source 5 irradiates through the detection holes, the spectrometer 13 collects the light beam to analyze and obtain the result.

[0025] Please refer to Figures 5-8In the embodiment, the fluorescence immune detection module comprises a lead screw motor 14 mounted on the fluorescence support 3, the output shaft of the lead screw motor 14 is provided with a thread and is connected with a detection table 15 through the thread, the detection table 15 is provided with detection positions, and the detection positions are three, which are a single reagent strip position 16, a double reagent strip position 17 and a triple reagent strip position 18. The optical module comprises a box bin 19, the upper end of the detection table 15 is provided with the box bin 19, the box bin 19 is internally mounted with an LED light source 20, and a receiving diode 21 is mounted in the box bin 19 and located at one side of the LED light source 20. The detection table 15 is fixed on the fluorescence detection support and is driven by the lead screw motor 14, so that the in-out movement of the detection table 15 is realized. The detection table 15 has three detection positions, and single reagent strip detection, double reagent strip detection and triple reagent strip detection can be realized. During detection, the reagent strip is inserted into the detection table 15, the tail end of the reagent strip is printed with a two-dimensional barcode, the barcode scanner 6 is fixed on the fluorescence detection support, the barcode scanner 6 identifies the barcode information on the reagent strip, the lead screw motor 14 drives the detection table 15 to move inward, and then moves outward. When passing through the detection position, the LED light source 20 in the optical module emits excitation light, which is irradiated on the fluorescence immune complex on the reagent strip, excites the complex, the complex emits fluorescence, the receiving diode 21 collects the fluorescence, and the conclusion is analyzed. In front of the instrument, a printer is also configured, and after detection is completed, the detected result can be printed out.

[0026] During microfluidic detection, the access cabin 8 is extended, the operator places the detection chip 10 on the fixing seat 9, at this time, the magnet makes the detection chip 10 and the fixing seat 9 attract together, in the process that the detection chip 10 and the fixing seat 9 attract together, the dilution cup film in the detection chip 10 is automatically torn, the dilution liquid flows into the detection chip 10, at the same time, the fixing seat 9 is provided with a ring tooth, which is engaged with the tooth on the detection chip 10, so as to realize the circumferential locking of the detection chip 10. When the access cabin 8 is retracted, the detection chip 10 is taken into the detection chamber 7, at this time, the NFC detection structure 12 reads the information of the NFC chip 11, after the information reading is completed, the detection chip 10 is driven to perform centrifugal movement by the direct-current brushless motor, after the centrifugal movement is completed, the sample and the dilution liquid will flow into the detection holes around the detection chip 10 and react with the freeze-dried balls in the holes. The light source 5 irradiates through the detection holes, the spectrometer 13 collects the light beam and analyzes to obtain the result.

[0027] When the fluorescence immunoassay is detected, different types of reagent strips are inserted into the corresponding detection positions, the tail end of the reagent strip is printed with a two-dimensional barcode, the barcode scanner 6 identifies the barcode information on the reagent strip, the lead screw motor 14 drives the detection table 15 to move inward, and then moves outward, when passing through the detection position, the LED light source 20 emits excitation light, which is irradiated on the fluorescence immunoassay compound on the reagent strip, excites the compound, the compound emits fluorescence, and the receiving diode 21 collects the fluorescence, and the analysis draws a conclusion.

[0028] Through the above steps, compared with the traditional single-function detection equipment, the biochemical and fluorescence immunoassay dual detection function is realized, the detection efficiency and comprehensiveness are greatly improved, and three different specifications of reagent strips can be detected at the same time, further enhancing the flexibility and convenience of detection, in addition, the device is also equipped with a barcode scanner 6 and an NFC detection structure 12, which can automatically read the information of the detection chip 10 or the reagent strip, simplifying the operation process and reducing human error, solving the problem that the existing microfluidic and fluorescence immunoassay analyzers only have single function and lack the dual detection capability of microfluidic and fluorescence immunoassay, resulting in limited practicality.

[0029] The embodiments of the utility model are described in detail above combined with the drawings, but the utility model is not limited to the above-mentioned embodiments, within the knowledge range of the person skilled in the art, various changes can be made without departing from the purpose of the utility model.

Claims

1. A portable biochemical coagulation and fluorescence immuno-integrated analysis device, comprising a shell (1), the shell (1) is internally provided with a power supply; characterized in that: The shell (1) is provided with two supporting plates (2), the upper end of the supporting plate (2) is fixedly connected with a fluorescent support (3), the shell (1) is provided with a support (4), the upper end of the support (4) is provided with a light source (5), the support (4) is provided with a micro-fluidic detection module, the upper end of the fluorescent support (3) is provided with a fluorescent immune detection module, the fluorescent immune detection module is provided with an optical module, one side of the optical module is provided with a confirmation detection chip (10) or a bar code scanner (6) for reagent strip information. 2.The portable biochemical coagulation and fluorescent immuno-integrated analysis device according to claim 1, characterized in that: The micro-fluidic detection module comprises a detection chamber (7), the detection chamber (7) is installed in the support (4), one side of the detection chamber (7) is provided with an in-out cabin (8) for conveying the detection chip (10), the in-out cabin (8) is provided with a fixing seat (9), the fixing seat (9) is provided with the detection chip (10), the detection chip (10) is provided with an NFC chip (11), the support (4) is provided with an NFC detection structure (12), and the lower end of the support is provided with a spectrometer (13). 3.The portable biochemical coagulation and fluorescent immuno-integrated analysis device according to claim 2, characterized in that: The upper end of the fixing seat (9) is provided with a magnet, the bottom of the magnet is inlaid with an iron sheet, and the detection chip (10) is connected with the fixing seat (9) through the magnet. 4.The portable biochemical coagulation and fluorescent immuno-integrated analysis device according to claim 3, characterized in that: The fluorescent immune detection module comprises a lead screw motor (14), the lead screw motor (14) is installed on the fluorescent support (3), the output shaft of the lead screw motor (14) is provided with a thread and is connected with a detection table (15), and the detection table (15) is provided with a detection position. 5.The portable biochemical coagulation and fluorescent immuno-integrated analysis device of claim 4, wherein: The detection position is provided with three kinds, which are single reagent strip position (16), double reagent strip position (17) and three reagent strip position (18). 6.The portable biochemical coagulation and fluorescent immuno-integrated analysis device according to claim 5, characterized in that: The optical module comprises a box (19), the upper end of the detection table (15) is provided with the box (19), the box (19) is provided with an LED light source (20), and the box (19) is provided with a receiving diode (21) and the receiving diode (21) is located on one side of the LED light source (20).

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