Equipment for preparing RNA-hyaluronic acid compound

Abstract

The utility model belongs to the technical field of RNA (Ribonucleic Acid) and hyaluronic acid compound preparation, and particularly relates to equipment for preparing an RNA-hyaluronic acid compound. Comprising a shell, a horizontal partition plate is fixedly arranged in the shell, the RNA centrifugal device comprises a centrifugal machine shell and a centrifugal cylinder, the centrifugal machine shell is arranged on the upper end face of the horizontal partition plate, and the centrifugal cylinder is rotationally arranged in the centrifugal machine shell; the compound mixing tank is arranged on the inner bottom surface of the shell and is communicated with the RNA centrifugal device through a pump machine and a pipeline. The RNA centrifugal collection device is provided with the RNA centrifugal device and the reaction tank, so that centrifugal collection of RNA can be realized. A centrifugal machine shell and a centrifugal barrel of the RNA centrifugal device are limited to a certain extent through a spring and a sleeve rod, and vibration in the centrifugal process can be reduced; and the charging pipe and the discharging pipe which are driven by the telescopic electric cylinder are arranged, so that a pipe orifice can be accurately moved to a target position, meanwhile, manual operation is reduced, and the automation level of equipment is improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to RNA and hyaluronic acid complex preparation technical field, specifically relates to a kind of equipment for preparing RNA-hyaluronic acid complex. BACKGROUND

[0002] RNA as the drug for promoting bone also has the following advantages: 1, can be directly applied, without developing new implant, thereby reducing R&D cost realizes quick clinical application;2, RNA molecule immunogenicity is lower, and biological safety is high. At the same time because the RNA molecular structure of vertebrate is homogeneous and can realize xenogeneic application. 3, RNA has the ability of combining other bioactive compounds, such as protein or antibiotic, etc., which is conducive to directly delivering drugs to implant area.

[0003] Hyaluronic acid shows a variety of important physiological functions in the body due to its unique molecular structure and physicochemical properties, such as lubricating joints, regulating the permeability of blood vessel wall, regulating protein, water and electrolyte diffusion and transport, promoting wound healing, etc.

[0004] The complex composed of RNA and hyaluronic acid can be well applied to implant, promote bone healing and development.

[0005] The existing application number "202020281990.1" "RNA extraction device" realizes the extraction of RNA by setting a separate centrifugal tube, and the existing patent application number "201922099198.4" "Hyaluronic acid mixing device" realizes the mixing of hyaluronic acid, but through retrieval, there is no complete set of equipment that can simultaneously integrate RNA extraction, reaction and RNA-hyaluronic acid mixing to finally realize the preparation of RNA-hyaluronic acid complex, thereby improving the preparation efficiency and can be applied on a large scale. Utility model content

[0006] The utility model aims at providing a kind of equipment for preparing RNA-hyaluronic acid complex, it can overcome the deficiency of above-mentioned prior art, by setting RNA centrifugal device and reaction tank, can realize the centrifugal collection of RNA. The centrifuge housing and centrifugal cylinder of RNA centrifugal device are limited by spring and sleeve rod, can reduce the vibration in the centrifugal process;Setting telescopic electric cylinder driven feeding pipe and discharge pipe, can accurately move the nozzle to target position, reduce manual operation at the same time, improve the automation level of equipment;Setting has complex mixture tank, complex mixture tank is provided with the blade of stirring motor drive, can realize the mixing of RNA and hyaluronic acid.In addition, the upper portion of complex mixture tank is provided with measuring cylinder, can collect and weigh the extracted RNA, can determine accurate addition amount when preparing RNA-hyaluronic acid complex, guarantee the treatment effect of product.

[0007] In order to achieve the above technical effects, the utility model adopts the technical scheme as follows:

[0008] A device for preparing RNA-hyaluronic acid complex, comprising a shell, a horizontal partition plate fixedly arranged in the shell, and further comprising:

[0009] An RNA centrifugal device, comprising a centrifuge shell and a centrifuge cylinder, the centrifuge shell being arranged at the upper end surface of the horizontal partition plate, and the centrifuge cylinder being rotatably arranged in the interior of the centrifuge shell;

[0010] A complex mixing tank, which is arranged at the inner bottom surface of the shell and is connected with the RNA centrifugal device through a pump and a pipeline.

[0011] Preferably, two telescopic cylinders are arranged on the outer surface of the centrifuge shell, one upper end of one telescopic cylinder is connected with a feeding pipe, and one upper end of the other telescopic cylinder is connected with a discharging pipe, and the lower ends of the feeding pipe and the discharging pipe can be inserted into the centrifuge cylinder.

[0012] Preferably, an inner rod is arranged on the outer wall of the centrifuge cylinder, a sleeve rod is sleeved on the inner rod, a roller is rotatably connected to the sleeve rod, a spring is further arranged on the inner rod, and a rolling groove for limiting the roller is arranged on the inner surface of the centrifuge shell.

[0013] Preferably, a measuring cylinder is connected to the upper part of the complex mixing tank, the upper part of the measuring cylinder is connected with the pump, and valves are arranged on the connecting pipes at the upper and lower ends of the measuring cylinder.

[0014] Preferably, an adding port is arranged on the upper end surface of the complex mixing tank, and a blade driven by a stirring motor is arranged in the interior.

[0015] 1. The utility model is provided with an RNA centrifugal device and a reaction tank, and can realize centrifugal collection of RNA. The centrifuge shell and the centrifuge cylinder of the RNA centrifugal device are limited through a spring and a sleeve rod, which can reduce vibration in the centrifugal process; the feeding pipe and the discharging pipe driven by telescopic cylinders can accurately move the pipe opening to a target position, and can reduce manual operation and improve the automation level of the equipment.

[0016] 2. The utility model is provided with a complex mixing tank, which is provided with a blade driven by a stirring motor and can realize mixing of RNA and hyaluronic acid. In addition, a measuring cylinder is arranged on the upper part of the complex mixing tank, which can collect and weigh the extracted RNA, can determine the accurate amount of addition when preparing the RNA-hyaluronic acid complex, and can ensure the treatment effect of the product. BRIEF DESCRIPTION OF DRAWINGS

[0017] The accompanying drawings are used to provide further understanding of the present application, and form a part of the specification, and are used to explain the present application together with embodiments of the present application, and do not constitute a limitation on the present application.

[0018] In the drawings:

[0019] Figure 1 It is a front view of the equipment of the present application;

[0020] Figure 2 It is a front view of the RNA centrifugal device of the present application;

[0021] Figure 3 It is Figure 2 It is an enlarged view of part A;

[0022] Figure 4 It is a front view of the compound mixing tank;

[0023] Figure 5 It is a sectional view of the inner rod;

[0024] Figure 6 It is a top view of the through hole.

[0025] 1. shell, 2. horizontal partition plate, 3. RNA centrifugal device, 301. centrifuge shell, 302. machine cover, 303. rotating motor, 304. centrifuge cylinder, 305. cylinder cover, 306. telescopic electric cylinder, 307. feeding pipe, 308. discharging pipe, 309. conduit, 310. inner rod, 311. rolling groove, 312. roller, 313. sleeve rod, 314. spring, 4. reaction tank, 5. compound mixing tank, 501. adding port, 502. stirring motor, 503. blade, 6. metering cylinder, 601. valve, 7. pump. DETAILED DESCRIPTION

[0026] The preferred embodiments of the present application are described below in conjunction with the accompanying drawings, and it should be understood that the preferred embodiments described herein are only used to explain and illustrate the present application, and do not constitute a limitation on the present application. EMBODIMENT

[0027] Refer to the accompanying Figures 1-6 An equipment for preparing RNA-hyaluronic acid complex, comprising a shell 1, the shell 1 is a cuboid structure, which is a steel frame structure of stainless steel material, a horizontal partition plate 2 is welded in the shell 1, and the inside of the shell 1 is divided into two parts.

[0028] The equipment further comprises:

[0029] RNA centrifuge device 3, the function of which is to collect RNA by centrifugation. RNA centrifuge device 3 includes centrifuge housing 301 and centrifuge tube 304. Centrifuge housing 301 can be fixed to the upper end face of horizontal partition plate 2 by welding or angle iron bolts. Rotary motor 303 is fixedly installed in the center of the inner bottom surface of centrifuge housing 301 by hinge and bolts. The output shaft of rotary motor 303 is connected to a hexagonal column. The inner bottom surface of centrifuge tube 304 is concavely formed with an internal hexagonal mounting hole that matches the hexagonal column. Centrifuge tube 304 is inserted into the hexagonal column of rotary motor 303, driving centrifugal movement of centrifuge tube 304 inside centrifuge housing 301.

[0030] To prevent the centrifuge cylinder 304 from shaking due to excessive rotation speed, an inner rod 310 is welded to the outer wall of the reaction vessel 4. The sleeve rod 313 has a U-shaped structure with hollow tubes at both ends. The inner rod 310 is fitted inside the hollow structure of the sleeve rod 313. To prevent the inner rod 310 and the sleeve rod 313 from slipping off, a limiting piece is fixedly installed at the end of the inner rod 310. The diameter of the limiting piece is larger than the diameter of the inner rod 310. A limiting ring is fixedly installed at the end of the sleeve rod 313. The outer diameter of the limiting ring is larger than the diameter of the sleeve rod 313, and the inner diameter of the limiting ring is larger than the diameter of the inner rod 310 but smaller than the diameter of the limiting piece.

[0031] Rollers 312 are rotatably mounted on the sleeve rod 313. In order to ensure a certain shock absorption function and to ensure that the rollers 312 contact the inner wall of the centrifuge housing 301, a spring 314 is provided on the inner rod 310. The spring 314 can push the sleeve rod 313 further.

[0032] Furthermore, a rolling groove 311 with a ring of limiting rollers 312 is formed on the inner surface of the centrifuge housing 301, which makes the centrifuge cylinder 304 more stable.

[0033] The upper part of the centrifuge cylinder 304 is threadedly connected to a cylinder cover 305. The end face of the cylinder cover 305 has two through holes, such as... Figure 6 As shown, a flexible rubber body is bonded inside the through hole. The flexible rubber body has a circular hole in the center. When the tube is inserted into the through hole, the flexible rubber body can be squeezed and passed through the central circular hole. Due to the properties of the flexible rubber body, it will be in a closed state when there is no tube squeezing.

[0034] The upper part of the centrifuge housing 301 is threadedly connected to the cover 302. The cover 302 has a through hole corresponding to the through hole of the cylinder cover 305. After the cover 302 is tightened, the tube can pass through the through hole of the cover 302 and the cylinder cover 305.

[0035] The outer surface of the centrifuge shell 301 is welded or fixed with angle iron bolt with two telescopic cylinders 306, the upper end of the two telescopic cylinders 306 is fixed with a cross arm, the cross arm of one of the telescopic cylinders 306 is connected with a feeding pipe 307, the upper end of the other telescopic cylinder 306 is connected with a discharging pipe 308, the lower end of the feeding pipe 307 and the discharging pipe 308 can be inserted into the centrifuge cylinder 304.

[0036] Further, in order to realize the replaceability of the feeding pipe 307 and the discharging pipe 308, a threaded hole is formed on the cross arm, the feeding pipe 307 and the discharging pipe 308 are made of hard stainless steel, the upper end of which is provided with external threads matched with the threaded hole, thereby realizing the connection of the feeding pipe 307 and the discharging pipe 308.

[0037] The upper part of the feeding pipe 307 is provided with a flared mouth, which facilitates feeding.

[0038] The upper end of the discharging pipe 308 is connected with a soft conduit 309, which is connected with the pump 7.

[0039] The composite mixing tank 5 is welded or fixed with angle iron bolt on the inner bottom surface of the shell 1.

[0040] The upper part of the composite mixing tank 5 is connected with a measuring cylinder 6 through a pipeline, and is connected with the reaction tank 4, in order to ensure the stability of the measuring cylinder, two L-shaped fixing rods 504 are symmetrically bonded on the side wall of the measuring cylinder 6, and the upper end of the fixing rod 504 is bonded on the lower end surface of the horizontal partition plate 2.

[0041] The upper end of the measuring cylinder 6 is communicated with the pump 4 through a pipeline, and a valve 601 is arranged on the connecting pipe at the lower end of the measuring cylinder 6.

[0042] The upper end surface of the composite mixing tank 5 is provided with a hyaluronic acid adding port 501, and the inside is provided with a blade 503 driven by a stirring motor 503. The side wall of the composite mixing tank 5 can be provided with an openable door, thereby facilitating the taking out of the mixed composite.

[0043] Use method:

[0044] In use, first, the left telescopic cylinder 306 is started, so that the feeding pipe 307 moves downward, the lower end is inserted into the barrel cover 305, RNA tissue is added, then TRIzol is added, then the telescopic cylinder 306 is moved upward, the feeding pipe 307 is out of the barrel cover 305, the rotating motor 303 is started, the centrifugal barrel 304 is driven to rotate, after mixing, 30 minutes are rested, TRIzol dissolves the RNA tissue, after shutdown, the operation is repeated, chloroform is added, the centrifugal barrel 304 is continuously rotated, TRIzol, RNA tissue and chloroform are mixed and centrifuged, finally, the liquid in the centrifugal barrel 304 is divided into three layers, the bottom is an organic phase layer, the middle is a protein layer, and the top is an RNA aqueous phase layer.

[0045] Then the right telescopic cylinder 306 is started, the discharge pipe 308 is driven to move downward to the bottom surface of the RNA aqueous phase layer, the pump 4 is started, the RNA aqueous phase layer is pumped into the measuring cylinder 6, according to the mixing ratio of RNA and hyaluronic acid, the hyaluronic acid is added from the adding port 501, the valve 601 is opened, and the RNA is added.

[0046] Finally, the stirring motor 502 is started, the RNA and the hyaluronic acid are mixed, and the RNA-hyaluronic acid complex is prepared.

[0047] The basic principle, main features and advantages of the utility model are shown and described. It should be understood by those skilled in the art that the utility model is not limited by the above examples, the above examples and the description in the specification are only to illustrate the principle of the utility model, various changes and improvements can be made to the utility model without departing from the spirit and scope of the utility model, and these changes and improvements all fall within the scope of the utility model claimed. The scope of protection of the utility model is defined by the appended claims and their equivalents.

Claims

1. An apparatus for preparing RNA-hyaluronic acid complex, comprising a housing (1), a horizontal partition plate (2) is fixedly arranged in the housing (1), characterized in that: Also include: RNA centrifugal device (3), including centrifuge shell (301) and centrifuge cylinder (304), centrifuge shell (301) is arranged on the upper end surface of horizontal partition plate (2), centrifuge cylinder (304) is arranged in the inside of centrifuge shell (301); Compound mixing tank (5), compound mixing tank (5) is arranged on the inner bottom surface of shell (1), is connected with RNA centrifugal device (3) through pump (4) and pipeline.

2. The apparatus for preparing an RNA-hyaluronic acid complex according to claim 1, characterized by: The outer surface of centrifuge shell (301) is provided with two telescopic cylinders (306), one of which is connected with feeding pipe (307) at the upper end, the other is connected with discharge pipe (308) at the upper end, the lower end of feeding pipe (307) and discharge pipe (308) can be inserted into centrifuge cylinder (304).

3. The apparatus for preparing an RNA-hyaluronan complex according to claim 2, wherein: The outer wall of centrifuge cylinder (304) is provided with inner rod (310), inner rod (310) is provided with sleeve rod (313), sleeve rod (313) is rotatably connected with roller (312), inner rod (310) is further provided with spring (314), the inner surface of centrifuge shell (301) is provided with a circle of rolling groove (311) limiting roller (312).

4. The apparatus for preparing an RNA-hyaluronic acid complex according to claim 3, characterized by: The upper part of compound mixing tank (5) is communicated with measuring cylinder (6), the upper part of measuring cylinder (6) is communicated with pump (4), valve (601) is arranged on the connecting pipe at the lower end of measuring cylinder (6).

5. The apparatus for preparing an RNA-hyaluronan complex according to claim 4, wherein: The upper end surface of compound mixing tank (5) is provided with hyaluronic acid adding port (501), and the inside is provided with blade (503) driven by stirring motor (502).

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