Novel multi-pit multi-layer cell ball culture bottle

Abstract

The utility model discloses a novel multi-pit multi-layer cell ball culture bottle, relates to the technical field of biological tissue engineering, and aims to solve the problem that the existing multi-layer cell culture bottle and a cell factory are established on the basis of plane adherent cell culture and cannot meet the existing three-dimensional cell culture requirement. The culture inner cavity is installed in the culture bottle main body, a plurality of multi-pit bottom plates are arranged in the culture inner cavity, an outer frame is arranged on a circle outside the plurality of multi-pit bottom plates, a culture three-dimensional groove body is arranged in the plurality of multi-pit bottom plates, and the culture three-dimensional groove body is arranged in the culture bottle main body. And a plurality of three-dimensional culture tank bodies are arranged.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological tissue engineering technical field, concretely is a new type cell ball multilayer culture bottle with multiple pits. BACKGROUND

[0002] Three-dimensional cell culture is an in vitro culture technique that simulates the microenvironment of cells in the body. It allows cells to grow and interact in three-dimensional space, forming cell structures and functions similar to those in the body. More and more evidence shows that three-dimensional cell culture can better simulate the in vivo environment, produce more cell models close to the physiological state, and even the gene expression profile of three-dimensional cultured cells reflects more accurate clinical expression information than single-layer cell culture. This three-dimensional cell culture method has a wide range of applications in drug screening, disease model construction, and tissue engineering. Among them, cell ball culture is a spherical cell aggregate formed by cell self-assembly, which secretes its own extracellular matrix more like a natural solid tissue, and has great potential in studying the growth and function of tumors and normal tissues. Cell balls can incorporate intercellular interactions, nutrient gradients, and diffusion kinetics into in vitro models, especially in cancer biology. Therefore, to better help achieve cell ball generation, culture, and amplification, cell ball mass production is urgently needed.

[0003] For example, the patent with the publication number CN219136772U, entitled "Multi-layer cell factory culture bottle", includes a top box, a bottom box, and multiple sets of culture bottles placed between the top box and the bottom box. Each set of culture bottles is a frame structure without a top end. Each set of culture bottles has a partition plate fixed to the inner wall of the front end, and a lower infiltration hole is provided on one side of the bottom surface of each set of culture bottles. The culture bottles are fixed by clamping. The two ends of the culture bottles, the bottom end of the top box, and the top end of the bottom box are fixed by clamping. At least one layer of culture bottles is arranged between the bottom box and the top box. The middle part of the top box is provided with a guide plate, and the guide plate extends to the side near the bottle opening and is provided with a longitudinal partition plate. Users can choose any number of culture bottles between the two according to specific use conditions. This design allows multiple cell culture spaces to be arranged between one bottom box and one top box, making the manufacturing cost low, the cell culture space efficient, and improving the utilization rate of space.

[0004] With the continuous innovation and rapid development of life science and technology, the large-scale in-vitro culture of cells for expressing monoclonal antibodies, specific proteins, interferons and viral vaccine products has become a common technology, and the multi-layer cell culture bottle and cell factory are a large-scale cell culture device which utilizes the maximum culture area in limited space, saves a large amount of factory space, and can reduce manual operation and reduce the risk of pollution. However, the existing multi-layer cell culture bottle and cell factory are based on the flat adherent cell culture, and cannot meet the existing culture requirements. Therefore, a multi-pit new cell ball multi-layer culture bottle is provided to solve the above problems. Content of the utility model

[0005] The utility model discloses a multi-pit new cell ball multi-layer culture bottle, which is used to solve the problem that the existing multi-layer cell culture bottle and cell factory are based on the flat adherent cell culture and cannot meet the existing culture requirements.

[0006] To achieve the above object, the utility model provides the following technical scheme: a multi-pit new cell ball multi-layer culture bottle, comprising a culture bottle main body, a connecting bottle mouth is arranged at one end of the culture bottle main body, a bottle mouth cover is arranged at one end of the connecting bottle mouth, and the bottle mouth cover is threadedly connected with the connecting bottle mouth.

[0007] Further comprising:

[0008] A culture inner cavity is installed in the inside of the culture bottle main body, a plurality of pit bottom plates are arranged in the inside of the culture inner cavity, an outer frame is arranged at one circle of the outside of the plurality of pit bottom plates, a plurality of culture three-dimensional groove bodies are arranged in the inside of the plurality of pit bottom plates, and the plurality of culture three-dimensional groove bodies are arranged in a plurality of.

[0009] Preferably, one end of the plurality of pit bottom plates is provided with a convex table, and the convex table is integrally formed with one end of the pit bottom plate.

[0010] Preferably, an upper plate layer is arranged above the inside of the culture inner cavity, and a mixing point is arranged on one side of one end of the culture inner cavity.

[0011] Preferably, both ends of the plurality of pit bottom plates are fixedly connected with the culture bottle main body.

[0012] Preferably, the plurality of culture three-dimensional groove bodies are integrally formed with the pit bottom plate.

[0013] Compared with the prior art, the utility model has the beneficial effects that:

[0014] 1. The utility model discloses a single layer or multilayer multi-pit bottom plate arranged in the inside of the culture bottle main body and a culture three-dimensional groove arranged on the end face of the multi-pit bottom plate, the bottom of the pit is hemispherical, inverted pyramid, inverted conical or hexagonal transition to hemispherical, the upper opening of the hemispherical or inverted conical is 50-3000 mu m in diameter range, the upper opening of the inverted pyramid is 50-3000 mu m in diagonal length range, the cell ball diameter obtained during subsequent culture is about 25-1500 mu m, all the inverted morphologies with large upper part and small lower part and all the sizes for cell ball culture under the morphologies can be effectively protected, the problem that the existing multilayer cell culture bottle and cell factory are all established on the basis of planar adherent cell culture and cannot satisfy the existing culture demand can be effectively avoided. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is the whole structure schematic diagram of the utility model;

[0016] Figure 2 It is the partial structure schematic diagram of the utility model's multi-pit bottom plate;

[0017] Figure 3 It is the enlarged schematic diagram of the structure A of the utility model;

[0018] In the drawing: 100, culture bottle main body; 101, connecting bottle mouth; 102, bottle mouth cover; 103, multi-pit bottom plate; 10301, culture three-dimensional groove; 10302, outer frame; 104, convex platform; 105, upper plate layer; 106, culture inner cavity; 107, mixing point. DETAILED DESCRIPTION

[0019] The technical scheme in the embodiments of the utility model will be apparently and completely described in combination with the drawings in the embodiments of the utility model, and obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments.

[0020] Embodiment 1

[0021] Please refer to Figures 1-3 The utility model provides an embodiment: multi-pit novel cell ball multilayer culture bottle, including: culture bottle main body 100, the one end of culture bottle main body 100 is provided with connecting bottle mouth 101, the one end of connecting bottle mouth 101 is provided with bottle mouth cover 102, and bottle mouth cover 102 is connected with connecting bottle mouth 101 by screw thread;

[0022] Still including:

[0023] The culture inner cavity 106 is installed in the inside of the culture bottle main body 100, the inside of the culture inner cavity 106 is provided with a plurality of pit bottom plates 103, and the plurality of pit bottom plates 103 are provided with a plurality of pit bottom plates 103. The outside of the plurality of pit bottom plates 103 is provided with an outer frame 10302, and the inside of the plurality of pit bottom plates 103 is provided with a culture three-dimensional groove body 10301, and the culture three-dimensional groove body 10301 is provided with a plurality of culture three-dimensional groove bodies 10301.

[0024] The designed multi-pit new type cell ball multi-layer culture bottle has single-layer or multi-layer multi-pit bottom plates 103, and each multi-pit bottom plate 103 has tens of thousands of cell ball culture three-dimensional groove bodies 10301, the bottom of the pit is hemispherical, inverted pyramid, inverted cone or hexagonal transition to hemispherical, the upper opening of the hemispherical or inverted conical shape is in the range of 50-3000 μm; the diagonal length of the upper opening of the inverted pyramid is in the range of 50-3000 μm, and the cell ball diameter obtained during subsequent culture is about 25-1500 μm, which can effectively protect all inverted morphologies and all sizes used for cell ball culture under the morphologies.

[0025] Embodiment 2

[0026] Please refer to Figure 1 and Figure 3 , one end of the plurality of pit bottom plates 103 is provided with a convex table 104, and the convex table 104 is integrally formed with one end of the pit bottom plate 103, the upper plate layer 105 is arranged in the upper part of the culture inner cavity 106, and the mixing point 107 is arranged on one side of one end of the culture inner cavity 106.

[0027] Please refer to Figure 1 , the two ends of the plurality of pit bottom plates 103 are fixedly connected with the culture bottle main body 100.

[0028] Please refer to Figure 2 and Figure 3 , the plurality of culture three-dimensional groove bodies 10301 are integrally formed with the plurality of pit bottom plates 103

[0029] Work steps: use a pipette or directly pour into the appropriate amount of cell suspension to the culture bottle main body 100, each layer of liquid volume is about 25-50ml. When the cell suspension is added, the pipette can be directly deep into the bottom of the culture bottle main body 100 or against the top slope, slowly add, try to avoid the generation of air bubbles, tighten the bottle cap 102, gently shake the bottle left and right, mix evenly, so that the liquid in each multi-pit bottom plate 103 in the culture three-dimensional groove body 10301 reaches balance, gently shake the culture bottle main body 100 before and after, so that the cell suspension is evenly distributed between each layer, quickly and gently put the culture bottle main body 100 on the side, mix the point upward, after the liquid surface is stationary, slowly put the culture bottle main body 100 flat from the side, the action needs to be gentle, prevent violent shaking to make the culture medium overflow from the layer, when harvesting cell balls, shake the culture bottle main body 100 bottle body left and right in the flat state, then put the bottle on the side, open the bottle cap 102, tilt the bottle body and pour the cell ball suspension into the centrifuge tube or collection bottle.

[0030] It is obvious to those skilled in the art that the present application is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or essential characteristics of the present application. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting, and the scope of the present application is defined by the appended claims rather than the above description, and therefore all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present application. Any reference signs in the claims should not be regarded as limiting the claims involved.

Claims

1. A multi-pit novel cell ball multi-layer culture flask, comprising a culture flask body (100), one end of the culture flask body (100) is provided with a connecting bottle mouth (101), one end of the connecting bottle mouth (101) is provided with a bottle mouth cover (102), and the bottle mouth cover (102) is threadedly connected with the connecting bottle mouth (101); characterized in that Further comprising: a culture inner cavity (106) installed in the inside of the culture flask body (100), the inside of the culture inner cavity (106) is provided with a plurality of multi-pit bottom plates (103), the outside of the plurality of multi-pit bottom plates (103) is provided with an outer frame (10302), and the inside of the plurality of multi-pit bottom plates (103) is provided with a plurality of culture three-dimensional groove bodies (10301).

2. The multi-pit novel cell sphere multi-layer culture flask of claim 1, wherein: One end of the plurality of multi-pit bottom plates (103) is provided with a convex table (104), and the convex table (104) is integrally formed with one end of the multi-pit bottom plate (103).

3. The multi-pit novel cell sphere multi-layer culture flask of claim 1, wherein: The upper part of the inside of the culture inner cavity (106) is provided with an upper plate layer (105), and one side of one end of the culture inner cavity (106) is provided with a mixing point (107).

4. The multi-pit novel cell sphere multi-layer culture flask of claim 1, wherein: Both ends of the plurality of multi-pit bottom plates (103) are fixedly connected with the culture flask body (100).

5. The multi-pit novel cell sphere multi-layer culture flask of claim 1, wherein: The plurality of culture three-dimensional groove bodies (10301) are integrally formed with the multi-pit bottom plate (103).

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