Circulating suction sample pretreatment device

Abstract

The utility model relates to the technical field of environmental sample treatment devices, and discloses a cyclic suction sample pretreatment device which comprises a heating vacuum chamber, reagent bottles are uniformly distributed in the heating vacuum chamber, a stationary phase filler storage device is assembled at the top of each reagent bottle, and a circulating suction device is assembled at the bottom of each stationary phase filler storage device. A needle tube is assembled at the top of the stationary phase filler storage device, a liquid level sensor is assembled outside the needle tube, an electromagnetic valve is assembled on the other side of the needle tube, the top of the needle tube is communicated with a connecting tube, the bottom of the connecting tube is communicated with a four-way valve, and the outside of the four-way valve is communicated with a vacuum pump; and the bottom of the four-way valve is communicated with the heating vacuum cabin. According to the cyclic suction sample pretreatment device, the influence of leakage of samples and impurities on subsequent operation is avoided, meanwhile, the stationary phase filler storage device is quickly assembled in a threaded connection mode, and the solvent consumption can be reduced and the extraction efficiency can be improved in a cyclic suction mode.

Description

TECHNICAL FIELD

[0001] The utility model relates to environmental sample processing device technical field, concretely is a kind of sample pretreatment device of cyclic suction. BACKGROUND

[0002] The content of pollutants in environmental samples is relatively low, and the matrix interference is complex, so appropriate sample pretreatment must be carried out before detection to ensure the accuracy and reliability of the analysis results. By removing impurities and interfering substances from the sample through sample pretreatment, background noise can be reduced, making it easier to detect and quantify target analytes, thereby improving the sensitivity and accuracy of the analysis. At the same time, sample pretreatment can enrich these low-concentration analytes or separate them from other substances to meet the analysis requirements.

[0003] The commonly used solid phase extraction (SPE) method is a sample pretreatment technology based on liquid-solid separation extraction. First, a solid adsorbent is used to adsorb the target substance in the liquid sample, separating the target substance from the sample. Then, an eluent is used to elute, achieving the separation and enrichment of the target substance. Finally, the eluted solution is concentrated, usually by evaporation or air flow concentration method, and the concentrated sample is dissolved in a suitable solvent for subsequent analysis, such as chromatographic analysis or mass spectrometric analysis. However, it has the problems of complex operation process, time-consuming processing process, large sample and solvent consumption, difficulty in automation, and easy plugging in the processing of complex samples. SUMMARY

[0004] (I) Technical problems solved

[0005] To solve the technical problems of complex operation process, time-consuming processing process, large sample and solvent consumption, difficulty in automation, and easy plugging in the processing of complex samples in the prior art, the utility model provides a sample pretreatment device with cyclic suction.

[0006] (II) Technical solutions

[0007] To achieve the above purpose, the utility model provides the following technical solutions: a sample pretreatment device with cyclic suction, comprising: a heated vacuum chamber, the inside of the heated vacuum chamber is uniformly provided with a reagent bottle, the top of the reagent bottle is equipped with a stationary phase filler storage device, and the top of the stationary phase filler storage device is equipped with a needle tube, the outside of the needle tube is equipped with a liquid level sensor, and the other side of the needle tube is equipped with a solenoid valve, the top of the needle tube is connected with a connecting pipe, the bottom of the connecting pipe is connected with a four-way valve, and the outside of the four-way valve is connected with a vacuum pump, the bottom of the four-way valve is connected with the heated vacuum chamber.

[0008] The fixed phase filler storage device comprises a storage tank and a rotating disc, both ends of the storage tank are connected with chucks, the outer part of the chuck is uniformly provided with curved grooves, the bottom of the curved groove is slidably connected with a round arm, the bottom of the round arm is connected with a closing plate, the other end of the closing plate is connected with a square block, the outer part of the rotating disc is provided with a hexagonal groove, and the hexagonal groove is slidably connected with the square block, and the bottom of the hexagonal groove is connected with a threaded cylinder.

[0009] Preferably, the reagent bottle comprises a bottle body, a needle is inserted into the inside of the bottle body, and a threaded connection port is assembled at the top of the needle, the inside of the reagent bottle can store a sample and is in communication with the fixed phase filler storage device and the needle tube.

[0010] Preferably, the heating vacuum chamber comprises a cavity, a heating plate is assembled at the inner bottom of the cavity, the heating vacuum chamber is a common vacuum device in the prior art, and is used for sealing the sample.

[0011] Preferably, the outside of the four-way valve is connected with a short connecting pipe through a flange plate, and a valve is assembled in the inside of the short connecting pipe, the short connecting pipe can control opening and closing and is in communication with the gas outside.

[0012] Preferably, the needle tube is connected with the connecting pipe through a flange plate, the bottom of the needle tube is threadedly connected with the threaded cylinder, the threaded cylinder can communicate the storage tank with the needle tube and the reagent bottle, and both ends in the inside of the storage tank can be assembled with filter plates.

[0013] Preferably, the closing plate has six groups, is uniformly arranged on the outer part of the chuck, and the six groups of closing plates move simultaneously, so that the opening and closing of both ends of the storage tank can be controlled.

[0014] (Three) beneficial effects

[0015] Compared with the prior art, the sample pretreatment device provided by the utility model has the following beneficial effects:

[0016] The circulating suction sample pretreatment device improves sample processing speed, reduces solvent consumption, improves extraction efficiency, realizes automation, and is suitable for required analysis instruments, and can realize automatic sample pretreatment integrating sample purification, enrichment, resolution and concentration, greatly improves sample processing speed, saves time for subsequent detection work, and the detachable stationary phase filler storage device avoids the problem of blockage in the device, and when the stationary phase filler storage device, the needle tube and the reagent bottle are disconnected, the two ends are automatically closed to avoid the falling of the internal sample or impurities, thereby avoiding pollution of the working environment and the influence of sample and impurity leakage on subsequent work, and the threaded connection method can quickly assemble the stationary phase filler storage device, greatly improve the use speed of the device, and the circulating suction method can reduce solvent consumption and improve extraction efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is a front view of the utility model;

[0018] Figure 2 It is a stationary phase filler storage device enlarged view of the utility model;

[0019] Figure 3 It is a chuck explosion view of the utility model;

[0020] Figure 4 It is a chuck enlarged view of the utility model.

[0021] In the figure: 1, heating vacuum chamber;2, reagent bottle;3, stationary phase filler storage device;31, storage tank;32, chuck;33, curved groove;34, round arm;35, closing plate;36, square;37, hexagonal groove;38, turntable;39, threaded cylinder;4, needle tube;5, liquid level sensor;6, electromagnetic valve;7, connecting pipe;8, four-way valve;81, short connecting pipe;9, vacuum pump. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the utility model.

[0023] The utility model provides a kind of technical scheme, please refer to Figure 1 And Figure 2The utility model provides a circulating suction sample pretreatment device, including: heating vacuum chamber 1, the inside of heating vacuum chamber 1 is uniformly arranged with reagent bottle 2, the top of reagent bottle 2 is equipped with stationary phase filler storage device 3, and the top of stationary phase filler storage device 3 is equipped with needle tube 4, the outside of needle tube 4 is equipped with liquid level sensor 5, and the other side of needle tube 4 is equipped with electromagnetic valve 6, the top of needle tube 4 is communicated with connecting pipe 7, the bottom of connecting pipe 7 is communicated with four-way valve 8, and the outside of four-way valve 8 is communicated with vacuum pump 9, and the bottom of four-way valve 8 is communicated with heating vacuum chamber 1.

[0024] Heating vacuum chamber 1 can seal reagent bottle 2, and the front of heating vacuum chamber 1 is equipped with a transparent plate for easy observation, the inside of reagent bottle 2 can store reagents, the inside of stationary phase filler storage device 3 can store fillers or samples, needle tube 4 can drive the sample, liquid level sensor 5 can control the movement of electromagnetic valve 6, electromagnetic valve 6 can control the movement of the sample, connecting pipe 7 can drive the movement of the sample, and four-way valve 8 can control the movement of the sample in cooperation with vacuum pump 9.

[0025] Please refer to Figure 3 and Figure 4 Stationary phase filler storage device 3 includes storage tank 31 and turntable 38, both ends of storage tank 31 are connected with chuck 32, the outside of chuck 32 is uniformly arranged with curved groove 33, the bottom of curved groove 33 is slidingly connected with circular arm 34, the bottom of circular arm 34 is connected with closure plate 35, the other end of closure plate 35 is connected with square block 36, the outside of turntable 38 is provided with hexagonal slot 37, and hexagonal slot 37 is slidingly connected with square block 36, and the bottom of hexagonal slot 37 is connected with threaded cylinder 39.

[0026] The inside of storage tank 31 can store samples and fillers, etc., and both ends of storage tank 31 can be equipped with filter screens, filter cartridges, etc., chuck 32 is fixedly assembled with storage tank 31, curved groove 33 can guide closure plate 35 in cooperation with circular arm 34, closure plate 35 can close chuck 32, and the cooperation of square block 36, hexagonal slot 37 and turntable 38 can drive closure plate 35 to move, threaded cylinder 39 can be threadedly connected with reagent bottle 2 or needle tube 4, and drive turntable 38 to transmit, and the bottom of chuck 32 is connected with a bearing with a seat through a hollow tube, and the bearing with a seat is connected with turntable 38.

[0027] Reagent bottle 2 includes a bottle body, a needle is inserted into the inside of the bottle body, and a threaded connection port is assembled at the top of the needle, the inside of reagent bottle 2 can store samples and be communicated with stationary phase filler storage device 3 and needle tube 4, heating vacuum chamber 1 includes a cavity, a heating plate is assembled at the inside bottom of the cavity, and heating vacuum chamber 1 is a common vacuum equipment in the prior art, which is used for sealing samples.

[0028] The four-way valve 8 is externally connected to a short pipe 81 via a flange, and a valve is installed inside the short pipe 81. The short pipe 81 can be controlled to open and close, and communicate with the external gas. The needle tube 4 is connected to the connecting pipe 7 via a flange, and the bottom of the needle tube 4 is threadedly connected to the threaded cylinder 39. The threaded cylinder 39 can connect the storage tank 31 with the needle tube 4 and the reagent bottle 2. Filter plates can be installed at both ends inside the storage tank 31. There are six sets of sealing plates 35, which are evenly distributed on the outside of the chuck 32. The six sets of sealing plates 35 move simultaneously, which can control the opening and closing of both ends of the storage tank 31.

[0029] This solution connects the two ends of the chuck 32 to the top of the reagent bottle 2 and the syringe 4 respectively, and rotates the threaded cylinder 39 to complete the connection between the stationary phase packing storage device 3, the reagent bottle 2 and the syringe 4. When the threaded cylinder 39 rotates, it drives the turntable 38 to rotate, and then drives the block 36 to move through the hexagonal groove 37. The block 36 drives the sealing plate 35 to move, thereby unsealing the bottom of the chuck 32. When disassembling the stationary phase packing storage device 3 from the reagent bottle 2 and the syringe 4, the above steps are reversed. At the same time, the two ends of the storage tank 31 are sealed to prevent impurities inside the stationary phase packing storage device 3 from falling out.

[0030] Mode 1: Cyclic Suction Mode

[0031] Liquid sample purification: The circulation and aspiration of the eluent or sample are controlled by solenoid valve 6. The specific usage procedure is as follows:

[0032] Connect the stationary phase packing storage device 3 to the reagent bottle 2 and the syringe 4. Put the sample solution into the reagent bottle 2 and place the reagent bottle 2 inside the heated vacuum chamber 1. Open the four-way valve 8 and start the vacuum pump 9 to evacuate the air. At this time, the inside of the heated vacuum chamber 1 is directly connected to the atmosphere, and the inside of the device is under negative pressure. The sample inside the reagent bottle 2 is drawn into the syringe 4 through the stationary phase packing storage device 3. When the liquid level rises to the outside of the liquid level sensor 5, the liquid level sensor 5 automatically drives the solenoid valve 6 to move, so that the inside of the reagent bottle 2 returns to atmospheric pressure. At this time, the sample flows back into the reagent bottle 2 according to its own gravity, and the solenoid valve 6 closes. The device starts again in the same way, and so on. The solenoid valve 6 can be adjusted to open and close automatically at the time frequency to achieve the effect of cyclic suction. Excess substances in the liquid sample are adsorbed into the inside of the stationary phase packing storage device 3, thereby purifying the liquid sample.

[0033] Liquid sample enrichment: Replace reagent bottle 2 and put a small amount of eluent into the replaced reagent bottle 2. Repeat the above steps to eluent the sample, thereby achieving the enrichment of the liquid sample.

[0034] Solid sample resolution: the fixed phase filler storage device 3 is taken out, the solid sample powder is put into the inside of the storage tank 31, and the fixed phase filler storage device 3 is assembled, the inside of the reagent bottle 2 is put into the resolution liquid, and the above steps are repeated, and the cycle is repeated, so as to realize the function of circulating suction, the target substance in the solid sample is eluted by the circulating resolution liquid, so as to realize the resolution of the solid sample.

[0035] Mode two: solid phase extraction mode

[0036] Switch the four-way valve 8, and form negative pressure in the heating vacuum chamber 1 by the vacuum pump 9, so as to realize the function of traditional solid phase extraction. The specific use process is as follows:

[0037] The fixed phase filler storage device 3, the reagent bottle 2 and the needle tube 4 are assembled, the electromagnetic valve 6 is opened, the sample solution is added in the inside of the needle tube 4, the reagent bottle 2 is opened and placed in the inside of the heating vacuum chamber 1, the vacuum pump 9 is opened to form negative pressure in the inside of the heating vacuum chamber 1, and the inside of the device is directly connected to the atmosphere. The liquid sample in the needle tube 4 slowly flows through the fixed phase filler storage device 3 and enters the inside of the reagent bottle 2 under the action of the negative pressure of the vacuum pump 9, so as to realize the function of traditional solid phase extraction, and the excess substances in the liquid sample are adsorbed in the fixed phase filler storage device 3, so as to realize the purification of the liquid sample.

[0038] The above steps are repeated, the electromagnetic valve 6 is opened again, the resolution liquid is added in the inside of the needle tube 4, and the above steps are repeated again to resolve the sample, so as to realize the enrichment of the liquid sample.

[0039] Mode three: nitrogen blowing concentration mode

[0040] After the enrichment, resolution and purification of the sample, the collected sample is in the inside of the reagent bottle 2. The device can directly realize further concentration for subsequent analysis. The short connecting pipe 81 is connected to the outside nitrogen, the vacuum pump 9 is opened to form negative pressure in the inside of the heating vacuum chamber 1, the nitrogen enters the inside of the reagent bottle 2 through the needle tube 4, the nitrogen is blown to the surface of the sample at a certain flow rate by using the inertness of the nitrogen, so that the solvent in the sample is quickly volatilized to achieve the purpose of concentrating the sample, and the function of nitrogen blowing is realized. At the same time, according to the stability of the sample, the heating module at the bottom of the heating vacuum chamber 1 is selected to be opened, so as to further concentrate the sample solution.

[0041] It is to be noted that, in the present document, relational terms such as first and second and the like can be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises", "comprising", or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus.

[0042] While the embodiments of the present application have been illustrated and described, it will be understood by those skilled in the art that various changes, modifications, substitutions, and alterations can be made therein without departing from the spirit and scope of the application, which is defined by the appended claims and their equivalents.

Claims

1. A cyclically aspirating sample preparation device comprising: Heating vacuum chamber (1), characterized in that: the inside of the heating vacuum chamber (1) is uniformly provided with reagent bottles (2), the top of the reagent bottles (2) is equipped with fixed phase filler storage device (3), and the top of the fixed phase filler storage device (3) is equipped with needle tube (4), the outside of the needle tube (4) is equipped with liquid level sensor (5), and the other side of the needle tube (4) is equipped with electromagnetic valve (6), the top of the needle tube (4) is communicated with connecting pipe (7), the bottom of the connecting pipe (7) is communicated with four-way valve (8), and the outside of the four-way valve (8) is communicated with vacuum pump (9), the bottom of the four-way valve (8) is communicated with heating vacuum chamber (1); The fixed phase filler storage device (3) comprises a storage tank (31) and a turntable (38), both ends of the storage tank (31) are connected with chucks (32), the outside of the chuck (32) is uniformly provided with curved grooves (33), the bottom of the curved groove (33) is slidably connected with a circular arm (34), the bottom of the circular arm (34) is connected with a closure plate (35), the other end of the closure plate (35) is connected with a block (36), the outside of the turntable (38) is provided with hexagonal grooves (37), and the hexagonal grooves (37) are slidably connected with the block (36), the bottom of the hexagonal groove (37) is connected with a threaded cylinder (39).

2. A recirculating pumped sample preparation device according to claim 1, wherein: The reagent bottle (2) comprises a bottle body, a needle is inserted into the inside of the bottle body, and a threaded connection port is arranged at the top of the needle.

3. A recirculating pumped sample preparation device according to claim 1, wherein: The heating vacuum chamber (1) comprises a cavity, and a heating plate is arranged at the inner bottom of the cavity.

4. The recirculating pumped sample preparation device of claim 1, wherein: The outside of the four-way valve (8) is connected with a short connecting pipe (81) through a flange plate, and the inside of the short connecting pipe (81) is equipped with a valve.

5. The recirculating pumped sample preparation device of claim 1, wherein: The needle tube (4) is connected with the connecting pipe (7) through a flange plate, and the bottom of the needle tube (4) is threadedly connected with the threaded cylinder (39).

6. The recirculating pumped sample preparation device of claim 1, wherein: The closure plate (35) has six groups, which are uniformly arranged on the outside of the chuck (32).

Images