Liquid flow device of flow cytometer
By combining a nozzle, a fixed support, a capillary tube, a rubber ball, and a pressure vessel, the problem of stable focusing in the flow cytometer's liquid flow device was solved, achieving constant flow rate and stable focusing of liquid samples, and improving the signal-to-noise ratio of the detection signal.
Patent Information
- Application Number
- CN202423200365.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-25
- Publication Date
- 2025-12-19
- Estimated Expiration
- 2034-12-25
AI Technical Summary
Existing flow cytometers with fluid flow devices have high requirements for positioning accuracy and strict sealing during the focusing process of sheath fluid and test sample, resulting in poor signal-to-noise ratio of the detection signal and unstable sheath fluid flow, which affects the detection effect.
The system employs a combination of nozzle, fixed support, capillary, rubber ball, and pressure vessel. The cell fluid inside the rubber ball is fixed by the right end of the capillary, and the pressure vessel provides constant pressure and constant flow to ensure that the liquid is sprayed out evenly and kept in the center of the liquid column, thus achieving stable liquid flow.
Stable focused flow of liquid samples at a constant flow rate was achieved, improving the signal-to-noise ratio and detection effect of the detection signal.
Smart Images
Figure CN223692219U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to a liquid flow device for multi-parameter detection and analysis of microparticles in liquid samples, in particular to a liquid flow device of a flow cytometer. BACKGROUND
[0002] Currently, there are two types of methods for detecting and analyzing cells or microparticles: one is a static method, which involves single or multiple layer processing of the sample (containing cells or other microparticles or thin layer tissue liquid), and then pressing it on a thin sheet (such as a glass slide) for detection and analysis of various performance characterization parameters, and the instruments used are generally ordinary optical microscopes, fluorescence microscopes or laser confocal microscopes, etc.; the other is a dynamic method, which involves flowing the cells or other microparticles in the sample through a specific detection area one by one at a constant speed, observing the various performance characterization parameters of each cell or other microparticle flowing through the detection area by using a detection device (such as a photomultiplier tube, avalanche diode, etc.), and using a computer to process and classify the observed results, which is the working principle of a flow cytometer. This is introduced in "Practical Flow Cytometry" by Shapiro and "Basic Principles and Practical Technology of Flow Cytometry" by Liang Zhihui et al. In a flow cytometer, to achieve the flowing of cells or other microparticles in the sample through a specific detection area one by one at a constant speed, the commonly used method is to use the laminar flow "focusing" force of the external sheath liquid (such as physiological saline) to achieve it. The sheath liquid-sample "focusing" core device of a general flow cytometer is as follows: Figure 1As shown, the sheath liquid gradually forms a "focused" laminar flow in the cavity C8 of the original lower segment flow cell 27, and the sample to be measured is gradually compressed under the "focusing" force of the laminar flow of the sheath liquid and forms a constant flow rate in the cavity C7 of the original upper segment flow cell 26. The liquid flow "focusing" device of the split flow cell requires high positioning accuracy of the center holes of the original upper segment flow cell 26 and the original lower segment flow cell 27, high mirror surface processing process requirement, and high sealing requirement. In the case of not achieving mirror surface leak-free fitting, a sealing gasket must be placed between the original upper segment flow cell 26 and the original lower segment flow cell 27, which will undoubtedly affect the stable laminar flow of the sheath liquid that has been formed at the outlet of the cavity C8 of the original lower segment flow cell 27, and further affect the "focused" flow of the sample to be measured. When the positioning of the center holes of the original upper segment flow cell 26 and the original lower segment flow cell 27 is not ideal, the "focused" position of the sample to be measured in the cavity C7 of the original upper segment flow cell 26 will be affected, deviating from the ideal position of the detection area, and further affecting the signal-to-noise ratio of the detection signal. In addition, this condition will also locally interfere with the stable laminar flow of the sheath liquid formed at the outlet of the cavity C8 of the original lower segment flow cell 27, and further affect the "focused" flow of the sample to be measured. Therefore, people need a liquid flow device using an integrated flow cell to achieve a "focused" flow of the sample to be measured forming a constant flow rate under the "focusing" force of the laminar flow of the sheath liquid. Content of the utility model
[0003] The utility model discloses a liquid flow device of flow cytometer, which solves the problems of the prior art.
[0004] The above technical purpose of the utility model is realized by the following technical scheme.
[0005] The liquid flow device of a flow cytometer comprises a nozzle, a fixed support, a capillary, a rubber ball and a pressure container, the nozzle is fixedly installed on the top of the fixed support, the nozzle is horizontally arranged from left to right, the nozzle is sequentially provided with an ejection part, a flow guide part, a flow stabilizing part and a liquid inlet part from left to right, the nozzle is internally provided with a central channel, the central channel is a first cylindrical channel in the ejection part, the central channel is a circular truncated cone channel in the flow guide part, the central channel is a second cylindrical channel in the flow stabilizing part, the central channel is a third cylindrical channel in the liquid inlet part, the diameter of the first cylindrical channel is 0.5mm, the left end of the circular truncated cone channel is consistent with the diameter of the first cylindrical channel, the right end of the circular truncated cone channel is consistent with the diameter of the second cylindrical channel, the diameter of the second cylindrical channel is 5mm, the diameter of the third cylindrical channel is 10mm, and the left end of the third cylindrical channel is connected with the right end of the second cylindrical channel, and a transition inclined surface is arranged at the connection position, the capillary is coaxially arranged in the liquid inlet part of the nozzle, the right end of the liquid inlet part of the nozzle is screwed with an end cover, the capillary is provided with an extension part extending through the end cover and extending to the right side of the end cover, a water inlet nozzle is arranged at the central position of the end cover and communicates with the central channel, one end of the capillary located at the right side of the end cover communicates with the rubber ball, the inside of the rubber ball is provided with cell liquid, the pressure container communicates with the right end of the water inlet nozzle, and the pressure container is used for providing constant pressure and constant flow liquid in the central channel.
[0006] By adopting the above technical scheme, the cell liquid in the rubber ball is fixedly installed at the right end of the capillary, and the constant pressure and constant flow liquid in the pressure container is driven to be uniformly and continuously sprayed out from the left side of the nozzle, and the cell liquid is wrapped in the center of the liquid column, so that the observation equipment can continuously observe the cells in the cell liquid in the center of the liquid column.
[0007] In further embodiments, the capillary is composed of a first horizontal pipe part and a second horizontal pipe part, the first horizontal pipe part is completely located in the nozzle, and the first horizontal pipe part is coaxial with the third cylindrical channel of the liquid inlet part of the nozzle, one end of the second horizontal pipe part is located in the nozzle, and the other end of the second horizontal pipe part is located outside the right side of the nozzle, the one end of the second horizontal pipe part located in the nozzle communicates with the first horizontal pipe part, and the other end of the second horizontal pipe part located outside the nozzle communicates with the inside of the rubber ball, and the included angle between the first horizontal pipe part and the second horizontal pipe part is 130°-150°.
[0008] By adopting the above technical scheme, the path conflict of the capillary and the water inlet nozzle at the end cover is avoided.
[0009] In further embodiments, the left end of the first horizontal pipe part is provided as a sharp angle.
[0010] In a further embodiment, the pressure vessel includes a fixed base, a water tank, and a lifting device. The water tank is fixedly installed on the top of the fixed base, and the lifting device is fixedly installed on the top of the water tank. A piston is slidably mounted inside the water tank. The top of the piston is fixedly connected to the slider of the lifting device via a connecting rod. The lifting device is used to drive the piston to move up and down inside the water tank via the connecting rod.
[0011] In a further embodiment, the lifting device is a servo hydraulic cylinder.
[0012] By adopting the above technical solution, the servo cylinder has higher precision than the pneumatic cylinder, which is more conducive to controlling the pressure inside the water tank through the piston.
[0013] In a further embodiment, a buffer pad is provided at the bottom of the fixing bracket.
[0014] In summary, this utility model has the following beneficial effects:
[0015] 1. By fixing the cell fluid inside the rubber ball at the right end of the capillary tube, the cell fluid is uniformly and continuously ejected from the left side of the nozzle under the constant pressure and constant flow of the liquid applied to the nozzle by the pressure vessel. The cell fluid is then carried by the constant pressure and constant flow of the liquid and is contained in the center of the liquid column, so that the observation device can continuously observe the effect of the cells in the cell fluid at the center of the liquid column. Attached Figure Description
[0016] Figure 1 This is a schematic diagram of the overall structure of this utility model.
[0017] In the diagram, 1 is the nozzle; 2 is the fixed bracket; 4 is the capillary tube; 5 is the rubber ball; and 6 is the pressure vessel. Detailed Implementation
[0018] The present invention will be further described in detail below with reference to the accompanying drawings.
[0019] Identical parts are indicated by the same reference numerals. It should be noted that the terms "front," "rear," "left," "right," "upper," and "lower" used in the following description refer to the attached figures. Figure 1 In this specification, the terms "bottom surface" and "top surface," "inner" and "outer" refer to the direction toward or away from the geometry of a specific component. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this specification, "a plurality of" means two or more, unless otherwise explicitly and specifically defined by the direction of the center.
[0020] Embodiment 1:
[0021] As Figure 1 shown, a liquid flow device of a flow cytometer includes a nozzle 1, a fixed support 2, a capillary tube 4, a rubber ball 5 and a pressure container 6. The nozzle 1 is fixedly installed on the top of the fixed support 2, and is horizontally arranged from left to right. The nozzle 1 is sequentially arranged from left to right as an ejection part, a flow guiding part, a flow stabilizing part and a liquid inlet part. The nozzle 1 is internally provided with a central passage. The central passage is a first cylindrical passage in the ejection part, a circular truncated cone passage in the flow guiding part, a second cylindrical passage in the flow stabilizing part, and a third cylindrical passage in the liquid inlet part. The diameter of the first cylindrical passage is 0.5 mm. The left end of the circular truncated cone passage is consistent with the diameter of the first cylindrical passage. The right end of the circular truncated cone passage is consistent with the diameter of the second cylindrical passage. The diameter of the second cylindrical passage is 5 mm. The diameter of the third cylindrical passage is 10 mm. The left end of the third cylindrical passage is connected with the right end of the second cylindrical passage, and is provided with a transition inclined surface. The capillary tube 4 is coaxially arranged in the liquid inlet part of the nozzle 1. The right end of the liquid inlet part of the nozzle 1 is screwed with an end cover. The capillary tube 4 is provided with an extension part which penetrates through the end cover and extends to the right side of the end cover. The central position of the end cover is provided with a water inlet nozzle 1 which is communicated with the central passage. One end of the capillary tube 4 located on the right side of the end cover is communicated with the rubber ball 5. The inside of the rubber ball 5 is provided with cell liquid. The pressure container 6 is communicated with the right end of the water inlet nozzle 1. The pressure container 6 is used to provide constant pressure and constant flow liquid in the central passage. The capillary tube 4 is composed of an integrally formed first horizontal tube part and a second horizontal tube part. The first horizontal tube part is completely located in the nozzle 1, and is coaxial with the third cylindrical passage of the liquid inlet part of the nozzle 1. One end of the second horizontal tube part is located in the nozzle 1, and the other end of the second horizontal tube part is located outside the nozzle 1. One end of the second horizontal tube part located in the nozzle 1 is communicated with the first horizontal tube part. The other end of the second horizontal tube part located outside the nozzle 1 is communicated with the inside of the rubber ball 5. The included angle of the connection between the first horizontal tube part and the second horizontal tube part is 130°-150°. The left end of the first horizontal tube part is provided with a sharp corner. The pressure container 6 includes a fixed base, a water tank and a lifting device. The water tank is fixedly installed on the top of the fixed base. The lifting device is fixedly installed on the top of the water tank. A piston is slidably installed in the inside of the water tank. The top of the piston is fixedly connected with the sliding block of the lifting device through a connecting rod. The lifting device is used to drive the piston to reciprocatingly displace in the water tank through the connecting rod. The lifting device is a servo oil cylinder. The bottom of the fixed support 2 is provided with a buffer gasket.
[0022] In the embodiments disclosed by the utility model, the terms "mounting", "connection", "connecting", "fixing" and the like should be understood in a broad sense, for example, "connecting" can be fixed connection, or detachable connection, or integrally connected; "connection" can be direct connection, or indirect connection through an intermediate medium.
[0023] The specific embodiments are merely an explanation of the utility model, and are not a limitation of the utility model, and a person skilled in the art can make modifications to the embodiments without creative contribution according to the needs after reading the specification, but as long as it is within the scope of the claims of the utility model, it is protected by the patent law.
Claims
1. A fluid flow device for a flow cytometer, characterized in that: The device includes a nozzle (1), a fixed bracket (2), a capillary tube (4), a rubber ball (5), and a pressure vessel (6). The nozzle (1) is fixedly installed on the top of the fixed bracket (2). The nozzle (1) is horizontally arranged. From left to right, the nozzle (1) is arranged as a spray section, a guide section, a flow stabilizing section, and a liquid inlet section. The nozzle (1) has a central channel inside. The section of the central channel inside the spray section is a first cylindrical channel, the section inside the guide section is a frustum-shaped channel, the section inside the flow stabilizing section is a second cylindrical channel, and the section inside the liquid inlet section is a third cylindrical channel. The diameter of the first cylindrical channel is 0.5 mm. The left end of the frustum-shaped channel has the same diameter as the first cylindrical channel, and the right end of the frustum-shaped channel has the same diameter as the second cylindrical channel. The diameters of the channels are consistent. The diameter of the second cylindrical channel is 5 mm, and the diameter of the third cylindrical channel is 10 mm. The left end of the third cylindrical channel and the right end of the second cylindrical channel are connected to form a transition slope. The capillary (4) is coaxially mounted in the liquid inlet of the nozzle (1). The right end of the liquid inlet of the nozzle (1) is screwed with an end cap. The capillary (4) is provided with an extension that passes through the end cap and extends to the right side of the end cap. The water inlet nozzle (1) is provided at the center of the end cap. The water inlet nozzle (1) is connected to the central channel. The end of the capillary (4) located on the right side of the end cap is connected to the rubber ball (5). The inside of the rubber ball (5) is provided with cell fluid. The pressure vessel (6) is connected to the right end of the water inlet nozzle (1). The pressure vessel (6) is used to provide constant pressure and constant flow liquid to the inside of the central channel.
2. The fluid flow device for a flow cytometer according to claim 1, characterized in that: The capillary tube (4) is composed of an integrally formed first horizontal tube section and a second horizontal tube section. The first horizontal tube section is completely located inside the nozzle (1), and the first horizontal tube section is coaxial with the third cylindrical channel of the liquid inlet section of the nozzle (1). One end of the second horizontal tube section is located inside the nozzle (1), and the other end of the second horizontal tube section is located outside the right side of the nozzle (1). The end of the second horizontal tube section located inside the nozzle (1) is connected to the first horizontal tube section, and the end of the second horizontal tube section located outside the nozzle (1) is connected to the inside of the rubber ball (5). The angle between the connection between the first horizontal tube section and the second horizontal tube section is 130°-150°.
3. The fluid flow device for a flow cytometer according to claim 2, characterized in that: The left end of the first horizontal tube is set as a sharp corner.
4. The fluid flow device for a flow cytometer according to claim 1, characterized in that: The pressure vessel (6) includes a fixed base, a water tank, and a lifting device. The water tank is fixedly installed on the top of the fixed base, and the lifting device is fixedly installed on the top of the water tank. A piston is installed inside the water tank and slides up and down. The top of the piston is fixedly connected to the slider of the lifting device through a connecting rod. The lifting device is used to drive the piston to move up and down inside the water tank through the connecting rod.
5. The fluid flow device for a flow cytometer according to claim 4, characterized in that: The lifting device is a servo hydraulic cylinder.
6. The fluid flow device for a flow cytometer according to claim 1, characterized in that: The bottom of the fixed bracket (2) is provided with a buffer pad.