Hyaluronidase fermentation device
By combining mechanical stirring and gas stirring, the problems of uneven gas distribution and low steam utilization efficiency in traditional hyaluronidase fermentation devices have been solved, achieving uniformity and stability of the fermentation broth, improving product quality and fermentation efficiency, and reducing energy consumption.
Patent Information
- Application Number
- CN202422633739.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-30
- Publication Date
- 2025-12-23
- Estimated Expiration
- 2034-10-30
AI Technical Summary
Traditional hyaluronidase fermentation devices suffer from uneven gas distribution, poor stirring effect, low steam utilization efficiency, and high energy consumption, which affect the microbial growth environment and enzyme synthesis efficiency.
It employs a combination of mechanical and gas agitation, using a central shaft, air distribution plate, and air distribution cone to evenly distribute sterile air. Combined with the steam inlet and outlet structure, it achieves uniform steam distribution and utilization, and uses a jacket layer for temperature control and sterilization.
It improves the uniformity and stability of the fermentation broth, enhances product quality, reduces energy consumption, and improves the efficiency and safety of the fermentation process.
Smart Images
Figure CN223705551U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to fermentation device technical field, concretely relates to a hyaluronidase fermentation device. BACKGROUND
[0002] Hyaluronidase, as an important biological enzyme, has shown a wide application prospect in many fields such as biological medicine, cosmetics, food and tissue engineering. In particular, in the field of biological medicine, hyaluronidase is widely used in ophthalmic surgery, arthritis treatment and tumor treatment, and its market demand is increasing year by year. In order to meet the growing demand for hyaluronidase, the technology of industrial production of hyaluronidase is constantly improved and optimized. However, in the fermentation process of hyaluronidase, the supply of sterile air is crucial for the growth of microorganisms and the synthesis of enzymes. The traditional fermentation device usually adopts the bottom air inlet mode to directly introduce sterile air into the fermentation broth. However, this mode has the problem of uneven gas distribution. Because the bubbles in the fermentation broth are easy to merge and rise, the gas concentration in some areas is too high, and the gas concentration in some areas is too low, which seriously affects the growth environment of microorganisms and the synthesis efficiency of enzymes. In addition, the traditional stirring system also has the problem of poor stirring effect. In the fermentation process of hyaluronidase, the utilization of steam is also a problem that cannot be ignored. The traditional fermentation device usually uses a steam generator to provide steam for heating the fermentation broth and sterilization. However, these devices often have the problems of low steam utilization efficiency and high energy consumption. SUMMARY
[0003] The utility model provides a kind of hyaluronidase fermentation device, adopts the way of mechanical stirring and gas stirring combination, increases the uniformity of fermentation broth, improves the stability of fermentation process and product quality.
[0004] The technical scheme of the utility model is as follows:
[0005] The fermentation device of hyaluronidase comprises a tank body, a closed jacket layer is welded on the outer surface of the tank body, a closed water storage space is formed between the jacket layer and the outer surface of the tank body, an electric heating component is arranged on the inner wall of the water storage space, a cover body is arranged on the top of the tank body, a feeding port is arranged on the upper portion of the tank body, a discharging port is arranged on the bottom of the tank body, a cooling water inlet is arranged on the bottom of the cooling jacket, and a cooling water outlet is arranged on the top of the cooling jacket; a gas storage chamber is arranged on the top of the cover body, a gas inlet valve is arranged on one side of the gas storage chamber, a hollow central shaft is arranged in the tank body, the upper end of the central shaft penetrates through the gas storage chamber and is connected with a stirring motor, a plurality of gas inlet pipes are arranged in the gas storage chamber, the hollow portion of the central shaft is communicated with the gas storage chamber through the gas inlet pipes, and stirring paddles are arranged on the outer side of the central shaft; a gas distribution disc is arranged on the middle portion of the central shaft, and a gas distribution cone is arranged on the lower portion of the central shaft; the inner cavities of the gas distribution disc and the gas distribution cone are communicated with the inner cavity of the central shaft to receive sterile air; a plurality of air outlet holes are uniformly arranged on the bottom of the gas distribution disc to discharge air downward, and the gas distribution cone is in an inverted conical shape, and a plurality of air outlet holes are uniformly arranged on the conical surface of the gas distribution cone to discharge air obliquely downward.
[0006] Preferably, a plurality of steam inlet pipes are arranged on the inner wall of the tank body, the steam inlet pipes comprise vertical steam inlet pipes and horizontal steam inlet pipes, the bottom air outlet end of the vertical steam inlet pipes extends to the bottom of the tank body, the top of the horizontal steam inlet pipes penetrates through the tank body and the jacket layer and is connected with the steam inlet end of the top end of the horizontal steam inlet pipes, a tee pipe is arranged on the horizontal steam inlet pipes, the horizontal steam inlet pipes penetrate through two outlets of the tee pipe, an air outlet opening is arranged on the jacket layer below the horizontal steam inlet pipes and communicated with the inside of the horizontal steam inlet pipes, the air outlet opening is connected with the other outlet of the tee pipe through a connecting pipe, and the last end of the connecting pipe and the steam inlet pipe is provided with a control valve.
[0007] Preferably, the height of the air outlet opening is higher than the height of the water in the jacket layer.
[0008] Preferably, the stirring paddles comprise a connecting sleeve and a paddle blade, the connecting sleeve is fixedly arranged on the outer side of the central shaft, the paddle blade is obliquely arranged on the connecting sleeve, and a through hole is arranged on the paddle blade.
[0009] Preferably, the width of the paddle blade gradually decreases from the connecting sleeve to the end of the paddle blade, the through holes on the paddle blade are uniformly arranged along the length direction of the paddle blade, and the diameter of the through holes gradually increases from the connecting sleeve to the edge of the paddle blade.
[0010] Preferably, a steam outlet is arranged on the side of the top of the tank body, and the steam outlet is connected with an induced draft fan through a pipeline.
[0011] Compared with the prior art, the utility model has the following beneficial effects:
[0012] 1. The utility model discloses a closed jacket layer is welded on the tank body outer surface, and the closed water storage space is formed between the jacket layer and the tank body outer surface. The inner wall of the water storage space is provided with an electric heating component, and the power of the electric heating component can be adjusted to control the temperature inside the tank body. At the same time, the top and bottom of the jacket layer are respectively provided with a cooling water inlet and a cooling water outlet, and the temperature inside the tank body can be further adjusted by circulating cooling water to improve the stability and response speed of temperature control.
[0013] 2. The utility model discloses a hollow central shaft and stirring paddle structure. The upper end of the central shaft passes through the gas storage chamber and is connected with the stirring motor, the plurality of air inlet pipes are located inside the gas storage chamber, and the hollow part of the central shaft is communicated with the gas storage chamber through the air inlet pipe. The sterile air enters the central shaft through the air inlet pipe and is uniformly distributed into the fermentation liquid through the air distribution disc arranged in the middle part of the central shaft and the air distribution cone arranged in the lower part. The inner cavities of the air distribution disc and the air distribution cone are communicated with the inner cavity of the central shaft to receive sterile air and are discharged downward and obliquely downward through the uniformly arranged air outlet holes, so that the gas is uniformly distributed in the fermentation liquid.
[0014] 3. The stirring paddle adopts a connecting sleeve and a paddle structure, the paddle is obliquely installed on the connecting sleeve and is provided with a through hole, in the stirring process, the particles with a diameter smaller than the through hole can fall to the other side of the paddle through the through hole, and the particles with a larger diameter stay on one side of the paddle, so that the large particles are extruded with the stirred particles, the large particles are broken, and the particle diameter after stirring of the stirring device is reduced. The stirring motor can drive the fermentation liquid to be fully stirred and mixed.
[0015] 4. The utility model discloses a plurality of steam inlet pipes and steam outlet structures arranged on the inner wall of the tank body. The steam inlet pipe includes a vertical steam inlet pipe and a horizontal steam inlet pipe, the bottom air outlet end of the vertical steam inlet pipe extends to the bottom of the tank body to introduce steam into the fermentation liquid. The top of the horizontal steam inlet pipe penetrates the tank body and the jacket layer and is connected with the steam inlet end of the horizontal steam inlet pipe, and is connected with the steam outlet through a tee pipe. The height of the steam outlet is higher than the height of the water in the jacket layer to prevent the water in the jacket layer from entering the tank body. This structure not only realizes uniform distribution and utilization of steam in the fermentation liquid, but also improves steam utilization efficiency and reduces energy consumption through waste heat recovery.
[0016] 5. The utility model discloses a device that can be sterilized before formal use, without additional sterilization device, steam is introduced into the bottom of the tank body through the vertical steam inlet pipe, as the steam is introduced, the steam gradually rises from the bottom of the tank body, and the steam can cover the inner wall of the tank body for sterilization in all directions due to the uniform distribution of the plurality of steam inlet pipes on the inner wall of the tank body, so the sterilization effect is good.
[0017] 6. The utility model discloses still set up the exhaust port on the side of the tank top and connect with the induced draft fan through the pipeline. This design can discharge the waste gas in the tank in time and keep the air in the tank fresh and flowability, further improve the stability of fermentation process and product quality. BRIEF DESCRIPTION OF DRAWINGS
[0018] Figure 1 It is the structural schematic diagram of the utility model.
[0019] Figure 2 It is the utility model Figure 1 A part of the enlarged view.
[0020] In the drawing, 1, tank body;2, jacket layer;3, cover body;4, feed inlet;5, discharge outlet;6, cooling water inlet;7, cooling water outlet;8, air chamber;9, air inlet valve;10, central shaft;11, air inlet pipe;12, stirring paddle;1201, connecting sleeve;1202, paddle blade;13, air distribution disc;14, air distribution cone;15, steam inlet pipe;1501, vertical steam inlet pipe;1502, horizontal steam inlet pipe;1503, tee pipe;16, steam outlet;17, connecting pipe;18, exhaust port;19, stirring motor. DETAILED DESCRIPTION
[0021] In order to make the personnel in the technical field better understand the technical scheme in the utility model, the technical scheme in the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiment of the utility model. Obviously, the described embodiment is only a part of the embodiment of the utility model, not all. Based on the embodiment in the utility model, all other embodiments obtained by the ordinary skill in the art without creative labor should belong to the protection scope of the utility model.
[0022] Embodiment 1
[0023] Hyaluronidase fermentation device, including tank body 1, the outer surface of tank body 1 is welded with closed jacket layer 2, the closed water storage space is formed between the outer surface of jacket layer 2 and tank body 1, is provided with electric heating part on the inner wall of water storage space, the top of tank body 1 is provided with cover body 3, tank body 1 upper portion is provided with feed inlet 4, the bottom of tank body 1 is provided with discharge outlet 5, the bottom of cooling jacket is provided with cooling water inlet 6, and the top is provided with cooling water outlet 7;Tank body 1 top side is provided with exhaust port 18, and exhaust port 18 is connected with induced draft fan through pipeline.
[0024] The top of the cover 3 is provided with a gas storage chamber 8, one side of the gas storage chamber 8 is provided with an air inlet valve 9, a hollow central shaft 10 is arranged in the tank body 1, the upper end of the central shaft 10 penetrates the gas storage chamber 8 and is connected with a stirring motor 19, a plurality of air inlet pipes 11 are arranged inside the gas storage chamber 8, the hollow part of the central shaft 10 is communicated with the gas storage chamber 8 through the air inlet pipe 11, and a stirring paddle 12 is arranged outside the central shaft 10; the stirring paddle 12 comprises a connecting sleeve 1201 and a paddle blade 1202, the connecting sleeve 1201 is fixedly arranged outside the central shaft 10, and the paddle blade 1202 is obliquely arranged on the connecting sleeve 1201; and a through hole is arranged on the paddle blade 1202.
[0025] The width of the paddle blade 1202 gradually decreases from the connecting sleeve 1201 to the end of the paddle blade 1202, the through holes on the paddle blade 1202 are uniformly arranged along the length direction of the paddle blade 1202, and the diameter of the through holes gradually increases from the connecting sleeve 1201 to the edge of the paddle blade 1202.
[0026] A plurality of steam inlet pipes 15 are arranged on the inner wall of the tank body 1, the steam inlet pipe 15 comprises a vertical steam inlet pipe 1501 and a horizontal steam inlet pipe 1502, the bottom gas outlet end of the vertical steam inlet pipe 1501 extends to the bottom of the tank body 1, the top of the horizontal steam inlet pipe 1502 penetrates the tank body 1 and the jacket layer 2 and is connected with the steam inlet end of the top end of the horizontal steam inlet pipe 1502, a tee pipe 1503 is arranged on the horizontal steam inlet pipe 1502, the horizontal steam inlet pipe 1502 penetrates two outlets of the tee pipe 1503, and each horizontal steam inlet pipe 1502 penetrates the jacket layer 2 below and is provided with a steam outlet 16 in communication with the inside thereof, the steam outlet 16 is connected with the other outlet of the tee pipe 1503 through a connecting pipe 17, the connecting pipe 17 and the last end of the steam inlet pipe 15 are provided with a control valve, and the height of the steam outlet 16 is higher than the height of the water in the jacket layer 2.
[0027] The central shaft 10 is provided with a gas distribution disc 13 and a gas distribution cone 14, the inner cavities of the gas distribution disc 13 and the gas distribution cone 14 are communicated with the inner cavity of the central shaft 10 to receive sterile air; the bottom of the gas distribution disc 13 is uniformly provided with gas outlets to discharge air downward, and the gas distribution cone 14 is in an inverted conical shape and is uniformly provided with gas outlets on the conical surface to discharge air obliquely downward.
[0028] Working process: Before formal use, sterilize, start the electric heating part, heat the water storage space in the jacket layer 2 to generate steam, the steam enters the bottom of the tank body through the vertical steam inlet pipe, as the steam enters, the steam gradually rises from the bottom of the tank body 1, plus several vertical steam inlet pipes evenly distributed on the inner wall of the tank body, the steam can cover the inner wall of the tank body all-round sterilization, after sterilization, when the temperature in the tank body decreases to the appropriate temperature, add appropriate amount of culture medium and necessary nutrients into the tank body 1 through the feeding port 4 for the fermentation of hyaluronidase. Close all unnecessary valves, such as cooling water inlet 6 and cooling water outlet 7 may be closed at the initial stage, according to the temperature requirement in the fermentation process. Start the electric heating part, heat the water storage space in the jacket layer 2 to maintain the appropriate temperature in the tank body 1, promote the growth of microorganisms and the fermentation of enzymes. At the same time, start the stirring motor 19, drive the stirring paddle 12 to rotate through the central shaft 10, fully stir the culture medium, ensure the uniform distribution of nutrients, and improve the fermentation efficiency. Sterile air enters the hollow part of the central shaft 10 through the air inlet pipe 11, and then is uniformly released into the fermentation broth through the air outlet holes on the air distribution disc 13 and the air distribution cone 14, providing sufficient oxygen for microorganisms and bringing gas stirring effect in the fermentation process. When the temperature in the tank body 1 is too high, open the cooling water inlet 6 and the cooling water outlet 7, let the cooling water flow through the jacket layer 2 to take away the heat and maintain the appropriate fermentation temperature. In the fermentation process, the waste gas can be discharged through the steam outlet 18 at the top of the tank body 1, and connected to the induced draft fan through the pipeline to ensure the cleanliness of the fermentation environment.
Claims
1. A hyaluronidase fermentation apparatus, characterized in that, It includes a tank body (1), a closed jacket layer (2) welded to the outer surface of the tank body (1), a closed water storage space is formed between the jacket layer (2) and the outer surface of the tank body (1), an electric heating component is provided on the inner wall of the water storage space, a cover (3) is provided on the top of the tank body (1), a feed inlet (4) is provided on the upper part of the tank body (1), a discharge outlet (5) is provided on the bottom of the tank body (1), a cooling water inlet (6) is provided on the bottom of the cooling jacket, and a cooling water outlet (7) is provided on the top. A gas storage chamber (8) is provided on the top of the cover (3). An air inlet valve (9) is provided on one side of the gas storage chamber (8). A hollow central shaft (10) is provided inside the tank (1). The upper end of the central shaft (10) passes through the gas storage chamber (8) and is connected to the stirring motor (19). Multiple air inlet pipes (11) are located inside the gas storage chamber (8). The hollow part of the central shaft (10) is connected to the gas storage chamber (8) through the air inlet pipes (11). A stirring paddle (12) is provided on the outside of the central shaft (10). The central shaft (10) is provided with an air distribution plate (13) in the middle and an air distribution cone (14) in the lower part. The inner cavity of the air distribution plate (13) and the air distribution cone (14) is connected to the inner cavity of the central shaft (10) to receive sterile air. The bottom of the air distribution plate (13) is evenly provided with air outlet holes to discharge air downwards. The air distribution cone (14) is inverted cone shape and its cone surface is evenly provided with air outlet holes to discharge air obliquely downwards. Several steam inlet pipes (15) are provided on the inner wall of the tank body (1). The steam inlet pipes (15) include vertical steam inlet pipes (1501) and horizontal steam inlet pipes (1502). The bottom outlet of the vertical steam inlet pipe (1501) extends to the bottom of the tank body (1). The top of the horizontal steam inlet pipe (1502) passes through the tank body (1) and the jacket layer (2) and is connected to the steam inlet end of the top of the horizontal steam inlet pipe (1502). A tee is provided on the horizontal steam inlet pipe (1502). Pipe (1503), transverse steam inlet pipe (1502) passes through the two outlets of the three-way pipe (1503), each transverse steam inlet pipe (1502) passes through the jacket layer (2) directly below the jacket layer (2) and is provided with a steam outlet (16) communicating with its interior, the steam outlet (16) is connected to the other outlet of the three-way pipe (1503) through the connecting pipe (17), and the connecting pipe (17) and the steam inlet pipe (15) are provided with control valves at the very end; The height of the steam outlet (16) is higher than the height of the water inside the jacket layer (2); The stirring paddle (12) includes a connecting sleeve (1201) and a blade (1202). The connecting sleeve (1201) is fixedly disposed on the outside of the central shaft (10), and the blade (1202) is obliquely mounted on the connecting sleeve (1201). The blade (1202) is provided with a through hole. The blade width of the blade (1202) gradually decreases from the connecting sleeve (1201) to the end of the blade (1202). The through holes on the blade (1202) are evenly distributed along the length of the blade (1202), and the diameter of the through holes gradually increases from the connecting sleeve (1201) to the edge of the blade (1202).
2. The hyaluronidase fermentation apparatus as described in claim 1, characterized in that, The tank (1) has a steam vent (18) on the top side, and the steam vent (18) is connected to the induced draft fan through a pipeline.