Fine line device for experiment for detecting cell migration ability

Abstract

The utility model relates to a fine line device for an experiment for detecting cell migration ability, which comprises a fine line main body, the adhesive sheets are arranged at the two ends of the fine line main body, and adhesive protective layers are arranged on the adhesive sheets; and the two ends of the fine line main body exceed the adhesive sheet. The thin line main body with the structure can be conveniently adhered to a culture plate through the adhesive sheet and is also convenient to tear off, and a blank area is left after the thin line main body is torn off and is used for observing and recording the cell migration capability. The utility model provides a fine line device for an experiment for detecting cell migration ability, which is detachable, simple and convenient to operate, and capable of ensuring the consistency of scratch width and reducing damage to scratch edge cells and the bottom surface of a culture plate. The problems that scratches engraved by a gun head are inconsistent in width, and damage to cells and the bottom face of a culture plate is large are solved.

Description

Technical Field

[0001] This utility model belongs to the technical field of cell migration experimental equipment, specifically relating to a fine thread device for detecting cell migration ability. Background Technology

[0002] Cell migration ability testing is a commonly used experimental method in biology, the simplest of which is the scratch healing assay, which is widely used. The basic principle of this method is to artificially create a blank area, or "scratch," on cells that have grown into a monolayer of fused cells. The speed at which cells at the edge of the scratch enter the blank area and cause the "scratch" to heal is used to demonstrate the cell migration ability.

[0003] Generally, the cell scratch assay includes three steps: the creation of the "scratch", the acquisition of cell migration images, and data processing. The basic procedure is as follows: (1) Marking the culture plate: Use a marker pen to draw horizontal lines on the back of a 6-well plate with a ruler, making one line every 0.5-1 cm, passing through the wells, with at least three lines per well; (2) Plating the cells: After digestion during the logarithmic growth phase, resuspend the cell suspension and count the cells under a microscope, seeding approximately 5 × 10⁶ cells per well. 5 (3) Cell streaking: Use a 200μL sterile pipette tip to make a streak perpendicular to the horizontal line on the well plate, so that the streak intersects the marking line, and try to ensure that the width of each streak is consistent; (4) Aspirate the cell culture medium, rinse the well plate 2-3 times with PBS to wash away cell debris, add culture medium, take pictures and record, and analyze the experimental results based on the collected picture data.

[0004] However, this method has the drawback of being difficult to control manually, resulting in irregular scratches and potential damage to cells and the bottom of the culture plate, thus affecting experimental results. Currently, there is no readily available, commercially available, and cost-effective experimental equipment to meet the needs of cell migration ability experiments.

[0005] In existing technologies, for example, Chinese Patent 201620501797.8, published on October 12, 2016, discloses a device and consumable kit for scratch experiments. The device includes a culture vessel and an "I-shaped" cell culture insert. After cell plating, the insert is removed, leaving a scratch of uniform width. However, this device has a relatively complex structure, high production cost, and poor flexibility, making it unsuitable for batch experiments. Another example is Chinese Patent 201420818439.0, published on July 29, 2015, which discloses a cell scratch experiment culture device. This culture vessel has a silicone film strip at the bottom along its diameter. After cell plating, the film strip is peeled off to obtain a scratch of uniform width, improving experimental efficiency. However, this culture vessel has low flexibility, can only be used once, and is more expensive than detachable strips, making it unsuitable for batch experiments. For example, Chinese Patent 201720398757.X, published on January 30, 2018, discloses a cell migration ability detection tape. This tape is applied to the surface of the culture vessel before cell seeding and then peeled off after cell seeding, creating a scratched blank area, thus forming scratches of uniform width and improving experimental accuracy. However, this tape is relatively small, making it difficult to form scratches across the culture vessel, which is insufficient for cell migration experiments, and its production cost is also relatively high.

[0006] Therefore, there is an urgent need to invent an easy-to-operate, multi-specification, and low-cost experimental apparatus to meet the needs of cell migration ability experiments, but no such apparatus has been reported so far. Utility Model Content

[0007] To address the shortcomings of the existing technology, this invention provides a fine-thread device for detecting cell migration ability. It is detachable, easy to operate, and can ensure the consistency of scratch width, reducing damage to cells at the scratch edge and the bottom surface of the culture plate. This solves the problems of inconsistent scratch width and significant damage to cells and the bottom surface of the culture plate caused by using pipette tips.

[0008] The present invention is solved by the following technical solution.

[0009] A fine-thread device for detecting cell migration ability includes: a fine-thread body; adhesive sheets at both ends of the fine-thread body, the adhesive sheets having an adhesive protective layer; the two ends of the fine-thread body extend beyond the adhesive sheets. This structure allows the fine-thread body to be easily attached to a culture plate via the adhesive sheets and also easily removed, leaving a blank area for observing and recording cell migration ability.

[0010] In a preferred embodiment, the cross-section of the thin thread body is a rectangle with a length l and a width d, wherein the length l is 30mm~40mm and the width d is 0.25mm~1mm, which can be well adapted to cell culture plates of different specifications and has strong practicality.

[0011] In a preferred embodiment, the length l is 35 mm, and the width d is one of 0.25 mm, 0.5 mm, 0.75 mm, and 1 mm.

[0012] In a preferred embodiment, the main body of the thin thread is a cylindrical thread, which is convenient to manufacture and has strong versatility.

[0013] In a preferred embodiment, the main body of the fine thread is nylon thread, which has strong corrosion resistance.

[0014] In a preferred embodiment, the adhesive on the adhesive sheet is a medical adhesive, which is non-toxic to cells and avoids affecting the experiment.

[0015] Compared with the prior art, the present invention has the following beneficial effects: it provides a fine thread device for detecting cell migration ability, which is detachable, easy to operate, can ensure the consistency of scratch width, and reduce damage to cells at the scratch edge and the bottom surface of the culture plate, so as to solve the problems of inconsistent scratch width and large damage to cells and the bottom surface of the culture plate when using pipette tips. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the fine thread device in this utility model.

[0017] Figure 2 This is a schematic diagram showing the adhesive protective layer of the fine thread device in this utility model being lifted.

[0018] Figure 3 This is a schematic diagram showing the adhesive protective layer of the fine thread device in this utility model after it has been peeled off.

[0019] Figure 4 This is a schematic diagram illustrating the use of the fine thread device in this utility model. Detailed Implementation

[0020] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0021] In the following embodiments, the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0022] In the description of this utility model, it should be understood that the terms such as center, longitudinal, transverse, length, width, thickness, upper, lower, front, back, left, right, vertical, horizontal, top, bottom, inner, outer, clockwise, and counterclockwise, indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description; therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features shown. In the description of this utility model, unless otherwise expressly specified and limited, the terms "installation," "connection," "joining," etc., should be interpreted broadly, and those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0023] See Figures 1 to 4 This invention relates to a fine-thread device for detecting cell migration ability in experiments, comprising: a fine-thread body 1; adhesive sheets 2 disposed at both ends of the fine-thread body 1, the adhesive sheets 2 having an adhesive protective layer 3; the two ends of the fine-thread body 1 extending beyond the adhesive sheets 2. The fine-thread body 1 of this structure can be easily adhered to a culture plate via the adhesive sheets 2, and is also easy to remove, leaving a blank area for observing and recording cell migration ability after removal.

[0024] In a specific embodiment, the cross-section of the thin filament body 1 is a rectangle with a length l and a width d. The length l is 30mm~40mm, and the width d is 0.25mm~1mm, which can well adapt to cell culture plates of different sizes and has strong practicality. For example, the length l is 35mm, and the width d is one of 0.25mm, 0.5mm, 0.75mm, and 1mm, which can adapt to the different migration abilities of cells.

[0025] Furthermore, in the embodiments of this application, the main body 1 of the thin thread is a cylindrical thread, which is convenient to manufacture and has strong versatility. Also, the main body 1 of the thin thread is made of nylon, which has strong corrosion resistance; the adhesive on the adhesive sheet 2 is a medical adhesive, which is non-toxic to cells and avoids affecting the experiment.

[0026] During the experiment, remove the adhesive protective layer 3 and use the adhesive sheet 2 to fix the main body 1 of the filament to the incubator. This filament device is individually sterile packaged and used after opening. The main body 1 of the filament is made of nylon, which has a certain acid and alkali resistance and is colorless and transparent.

[0027] See Figure 4 Take the thin thread device out of the packaging and fix the thin thread body 1 in the center of the six-hole plate. Figure 4 China A and Figure 4(As shown in Figure B), spanning the diameter, unfold the adhesive sheets 2 at both ends of the thin thread body 1 and peel off the adhesive protective layer 3. Use the adhesive sheets 2 to tightly connect the thin thread body 1 to the culture vessel; inoculate cells for culture (e.g., ...). Figure 4 (As shown in C); after the cells have grown into a monolayer of adherent cells, use tweezers to peel off the adhesive tape and remove the thread, thus obtaining a regularly shaped scratch with undamaged edge cells (as shown in Figure C). Figure 4 (As shown in D), cell migration ability was then detected based on the image data.

[0028] The advantages of this fine thread device for cell migration experiments are: sterile and individually packaged to effectively prevent contamination; it can produce regularly shaped scratches without damaging cells or the bottom of the culture plate; it is suitable for various cell culture vessels and can be used immediately; it is made of waterproof material and does not delaminate after being soaked in water for 3 days; it is simple to operate, effectively reduces experimental costs, facilitates batch operations in experiments, and improves experimental efficiency.

[0029] As described above, this utility model provides a fine thread device for detecting cell migration ability. It is detachable, easy to operate, and can ensure the consistency of scratch width, reduce damage to cells at the scratch edge and the bottom surface of the culture plate, thereby solving the problems of inconsistent scratch width and significant damage to cells and the bottom surface of the culture plate caused by using pipette tips.

[0030] The scope of protection of this utility model includes, but is not limited to, the above embodiments. The scope of protection of this utility model is defined by the claims. Any substitutions, modifications, or improvements to this technology that are easily conceived by those skilled in the art shall fall within the scope of protection of this utility model.

Claims

1. A fine-thread apparatus for detecting cell migration ability in experiments, characterized in that, include: Thin line body (1); Adhesive sheets (2) are provided at both ends of the main body of the thin thread (1), and adhesive protective layers (3) are provided on the adhesive sheets (2). The two ends of the thin wire body (1) extend beyond the adhesive sheet (2).

2. The fine thread apparatus for detecting cell migration ability according to claim 1, characterized in that, The cross-section of the thin wire body (1) is a rectangle with a length l and a width d, wherein the length l is 30mm~40mm and the width d is 0.25mm~1mm.

3. The fine thread apparatus for detecting cell migration ability according to claim 2, characterized in that, The length l is 35mm, and the width d is one of 0.25mm, 0.5mm, 0.75mm, and 1mm.

4. The fine thread apparatus for detecting cell migration ability according to claim 1, characterized in that, The main body of the thin thread (1) is a cylindrical thread.

5. The fine-thread device for detecting cell migration ability according to claim 1, characterized in that, The main body of the thin thread (1) is a nylon thread.

6. The fine thread apparatus for detecting cell migration ability according to claim 1, characterized in that, The adhesive on the adhesive sheet (2) is a medical adhesive.

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