Controllable batch cell sample electric breakdown device
By designing a controllable batch cell sample electrolysis device, and utilizing a combination of multiple material orifices and electrode rods, simultaneous electrolysis of multiple sets of cell solutions was achieved, solving the problems of low efficiency and high cost in existing technologies, improving experimental efficiency and reducing consumable costs.
Patent Information
- Application Number
- CN202423247824.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-27
- Publication Date
- 2025-12-23
- Estimated Expiration
- 2034-12-27
AI Technical Summary
Existing technologies can only process one cell fluid sample at a time, which is inefficient, and the high price of electrode tubes or electrode cups leads to high laboratory usage costs.
Design a controllable batch cell sample electrolysis device, including a control module, a well plate and an electrode module. Multiple material holes are used in conjunction with electrode rods to achieve simultaneous electrolysis of multiple sets of cell solutions. Ninety-six electrode rods are divided into thirty-two control zones, and the device is easy to install or disassemble through snap-fit and slot connection.
It improves the efficiency of large-scale experimental electrolysis operations, reduces the cost of consumables, enables simultaneous electrolysis of multiple cell solutions, and facilitates high-temperature sterilization of the electrode module.
Smart Images
Figure CN223705601U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to microorganism processing technical field especially relates to a controllable batch cell sample's electric breakdown device. BACKGROUND
[0002] Electric breakdown also called electric osmosis, it is microbiology technology, high voltage electric field (300V-3000V) is applied to cell, transiently improves the permeability of cell membrane, thereby absorbs the exogenous molecule in surrounding medium, this technique can guide nucleotide, DNA and RNA, protein, saccharide, dye and virus particle etc into prokaryotic and eukaryotic cell, thereby transforms bacteria, yeast or plant protoplast.
[0003] Current common practice is to add cell liquid into electrode tube or electrode cup, and then put the electrode tube or electrode cup into special instrument for electrolysis, but this method has certain defects: only one cell liquid sample can be processed each time, and the efficiency is low; the price of each electrode tube or electrode cup is several yuan to tens of yuan, and the laboratory uses a large number of electrode tubes or electrode cups, which has high cost. UTILITY MODEL CONTENT
[0004] The utility model discloses a controllable batch cell sample's electric breakdown device, which can process multiple cell samples at the same time, and the efficiency is improved.
[0005] In order to realize the above-mentioned purpose, the utility model adopts the following technical scheme: a controllable batch cell sample's electric breakdown device, which comprises a control module, a hole plate and an electrode module, the control module is clamped on the top surface of the electrode module, the electrode module is movably connected to the top surface of the hole plate, the electrode module comprises an electrode rod and an insulating shell, the electrode rod is connected to the bottom surface of the insulating shell, a communication groove is formed through the outer wall of the electrode rod, an installation groove is formed in the insulating shell, an electric circuit board is fixedly installed on the inner wall of the installation groove, a conductive rod is sealingly connected to the top surface of the insulating shell, the bottom end of the conductive rod is connected to the top surface of the electric circuit board, the control module comprises a shell, a control circuit is installed on the inner wall of the shell, a telescopic probe is connected to the bottom surface of the shell, the control circuit and the telescopic probe are electrically connected, the bottom end of the telescopic probe is in pressure contact with the top end of the conductive rod, the hole plate comprises a bottom plate, a material hole and a limiting groove, the material hole is formed in the top surface of the bottom plate, the limiting groove is formed in the outer wall of the bottom plate, and the electrode rod is inserted into the material hole.
[0006] Preferably, the electrode rod comprises a conductive rod one, a conductive rod two, an electrode sheet and an insulating sleeve plate, the top end of the conductive rod one and the conductive rod two are inserted into the bottom surface of the circuit board, and the insulating sleeve plate is provided with two, and the two insulating sleeve plates are respectively sleeved on the outer walls of the conductive rod one and the conductive rod two.
[0007] Preferably, the electrode sheet is provided with two, the outer walls of the conductive rod one and the conductive rod two are respectively fixedly connected with the outer walls of the electrode sheet, and the two electrode sheets are respectively embedded on the two sides of the inner wall of the communication groove.
[0008] Preferably, the two end outer walls of the shell are provided with buckles, the two end outer walls of the insulating shell are provided with clamping grooves, the bottom end of the buckle is clamped in the inner wall of the clamping groove, and the control module is clamped with the electrode module through the buckle.
[0009] Preferably, the top surface of the shell is fixedly connected with a top cover, and the outer wall of the shell is connected with a navigation plug.
[0010] Preferably, the top surface of the insulating shell is provided with a positioning hole, the bottom surface of the shell is fixedly connected with a positioning pin, and the positioning pin is inserted into the inner wall of the positioning hole.
[0011] Compared with the prior art, the utility model has the advantages and positive effects that:
[0012] 1、 in the utility model, the cell liquid to be treated is injected into the material hole of the hole plate, then the electrode module is buckled on the top surface of the hole plate to make the electrode rod inserted into the material hole, the top surface of the control module is clamped with the electrode module to make the telescopic probe and the conductive rod top surface pressure contact connection, through the external controller, the electrode rod is electrified through the control circuit, the telescopic probe, the conductive rod and the circuit board, the electrolysis operation is realized by discharging the cell liquid in the material hole, and through the cooperation of the electrode rod and the plurality of material holes, the utility model can realize the synchronous electrolysis of multiple groups of cell liquid and improve the efficiency of batch experiment electrolysis operation.
[0013] 2、 in the utility model, through the setting of the electrode rod, the conductive rod and the circuit board, the utility model can make 96 electrode rods realize 32 control partitions, so that the user can control the partition electrolysis of the cell liquid in the material hole in different areas, and the setting of the navigation plug is convenient for the external controller of the utility model to control the utility model.
[0014] 3、 in the utility model, the clamping of the buckle and the clamping groove makes the control module and the electrode module can be conveniently installed or disassembled, so that the electrode module can be conveniently subjected to high-temperature sterilization treatment after use. BRIEF DESCRIPTION OF DRAWINGS
[0015] Figure 1The utility model provides a controllable batch cell sample's electric breakdown device's three-dimensional structure schematic diagram;
[0016] Figure 2 The utility model provides a controllable batch cell sample's electric breakdown device's structure explosion map;
[0017] Figure 3 The utility model provides a controllable batch cell sample's electric breakdown device control module's internal structure schematic diagram;
[0018] Figure 4 The utility model provides a controllable batch cell sample's electric breakdown device electrode module's internal structure schematic diagram;
[0019] Figure 5 The utility model provides a controllable batch cell sample's electric breakdown device electrode stick's internal structure schematic diagram;
[0020] Figure 6 The utility model provides a controllable batch cell sample's electric breakdown device electrode stick's arrangement structure schematic diagram.
[0021] Legend: 1, control module, 11, shell, 12, plug, 13, buckle, 14, top cover, 15, control circuit, 16, telescopic probe, 17, positioning pin, 2, aperture plate, 21, bottom plate, 22, material hole, 23, limiting groove, 3, electrode module, 31, electrode stick, 311, conductive stick one, 312, conductive stick two, 313, electrode sheet, 314, communication groove, 315, insulating cover plate, 32, insulating shell, 33, positioning hole, 34, conductive rod, 35, clamping groove, 36, installation groove, 37, circuit board. DETAILED DESCRIPTION
[0022] In order to more clearly understand the above purpose, features and advantages of the utility model, the utility model is further explained below in conjunction with the drawings and examples. It should be explained that, in the non-conflict situation, the embodiment of the application and the features in the example can be combined mutually.
[0023] In the following description, a lot of specific details are set forth in order to fully understand the utility model, but the utility model can also be implemented in other ways different from the description, therefore, the utility model is not limited to the specific embodiment of the following disclosure.
[0024] Example one: as Figure 1 - Figure 6The utility model provides a controllable batch cell sample's electric breakdown device, including control module 1, well plate 2 and electrode module 3, control module 1 is jointed in the top surface of electrode module 3, electrode module 3 swing joint is in the top surface of well plate 2, electrode module 3 includes electrode stick 31 and insulating shell 32, insulating shell 32 is made of epoxy resin material, electrode stick 31 is connected in the bottom surface of insulating shell 32, and the outer wall of electrode stick 31 is provided with the through groove 314, the through groove 314 is made of SUS316 stainless steel material, has good high temperature resistance, corrosion resistance, and the inside of insulating shell 32 is provided with the mounting groove 36, and the inner wall of mounting groove 36 is fixedly installed with circuit board 37, and the top surface of insulating shell 32 is connected with the electrically conductive rod 34, and the bottom end of electrically conductive rod 34 is connected in the top surface of circuit board 37, control module 1 includes the shell 11, and the inner wall of shell 11 is installed with control circuit 15, and the bottom surface of shell 11 is connected with telescopic probe 16, and control circuit 15 is electrically connected between telescopic probe 16, and the bottom end of telescopic probe 16 is connected with the top end of electrically conductive rod 34, well plate 2 includes bottom plate 21, material hole 22 and limiting slot 23, material hole 22 is set up in the top surface of bottom plate 21, and limiting slot 23 is set up in the outer wall of bottom plate 21, and electrode stick 31 is inserted in the inside of material hole 22.
[0025] The utility model discloses a controllable batch cell sample's electric breakdown device, including control module 1, well plate 2 and electrode module 3, control module 1 is jointed in the top surface of electrode module 3, electrode module 3 swing joint is in the top surface of well plate 2, electrode module 3 includes electrode stick 31 and insulating shell 32, insulating shell 32 is made of epoxy resin material, electrode stick 31 is connected in the bottom surface of insulating shell 32, and the outer wall of electrode stick 31 is provided with the through groove 314, the through groove 314 is made of SUS316 stainless steel material, has good high temperature resistance, corrosion resistance, and the inside of insulating shell 32 is provided with the mounting groove 36, and the inner wall of mounting groove 36 is fixedly installed with circuit board 37, and the top surface of insulating shell 32 is connected with the electrically conductive rod 34, and the bottom end of electrically conductive rod 34 is connected in the top surface of circuit board 37, control module 1 includes the shell 11, and the inner wall of shell 11 is installed with control circuit 15, and the bottom surface of shell 11 is connected with telescopic probe 16, and control circuit 15 is electrically connected between telescopic probe 16, and the bottom end of telescopic probe 16 is connected with the top end of electrically conductive rod 34, well plate 2 includes bottom plate 21, material hole 22 and limiting slot 23, material hole 22 is set up in the top surface of bottom plate 21, and limiting slot 23 is set up in the outer wall of bottom plate 21, and electrode stick 31 is inserted in the inside of material hole 22.
[0026] Embodiment two: as Figure 1 - Figure 6As shown, the electrode rod 31 comprises the conductive rod one 311, the conductive rod two 312, the electrode sheet 313 and the insulating sleeve plate 315, the top end of the conductive rod one 311 and the conductive rod two 312 are inserted into the bottom surface of the circuit board 37, the insulating sleeve plate 315 is provided with two, the two insulating sleeve plates 315 are respectively sleeved on the outer wall of the conductive rod one 311 and the conductive rod two 312, the electrode sheet 313 is provided with two, the outer wall of the conductive rod one 311 and the conductive rod two 312 is respectively fixedly connected with the outer wall of the electrode sheet 313, the two electrode sheets 313 are respectively embedded on the inner wall of the communication groove 314, the electrode rod 31 is provided with ninety-six, the ninety-six electrode rods 31 are divided into eight rows and twelve columns, the conductive rod one 311 or the conductive rod two 312 of each two rows of electrode rods 31 close to each other is of the same polarity, so that the breakdown between the adjacent electrode rods 31 after power-on is effectively avoided, the conductive rod 34 is provided with twelve and is divided into eight negative poles and four positive poles, the positive pole of every three columns of electrode rods 31 is electrically connected with a conductive contact 33, and the negative pole of each row of electrode rods 31 is electrically connected with a conductive contact 33, so that the ninety-six electrode rods 31 realize thirty-two control partitions, the outer wall of both ends of the shell 11 is provided with the buckle 13, the outer wall of both ends of the insulating shell 32 is provided with the clamping groove 35, the bottom end of the buckle 13 is clamped in the inner wall of the clamping groove 35, the control module 1 is clamped with the electrode module 3 through the buckle 13, the top surface of the shell 11 is fixedly connected with the top cover 14, the outer wall of the shell 11 is connected with the navigation plug 12 in a penetrating mode, the top surface of the insulating shell 32 is provided with the positioning hole 33, and the bottom surface of the shell 11 is fixedly connected with the positioning pin 17, and the positioning pin 17 is inserted into the inner wall of the positioning hole 33.
[0027] The whole embodiment achieves the effects that the conductive rod one 311 and the conductive rod two 312 are arranged, the circuit board 37 is arranged to conduct electricity to the two electrode sheets 313 through the conductive rod one 311 and the conductive rod two 312, so as to discharge the communication groove 314 to realize electrolysis of the cell solution, the electrode rod 31, the conductive rod 34 and the circuit board 37 are arranged, so that the ninety-six electrode rods 31 realize thirty-two control partitions, so that the user can conveniently control the electrolysis of the cell solution in the different area material holes 22, the buckle 13 and the clamping groove 35 are clamped, so that the control module 1 and the electrode module 3 can be conveniently installed or disassembled, so as to facilitate high-temperature sterilization treatment of the electrode module 3 after use, the positioning pin 17 and the positioning hole 33 are inserted, so that the control module 1 and the electrode module 3 are better positioned when clamped, and the connection is more accurate, and the navigation plug 12 is arranged, so that the external controller of the utility model can control the utility model.
[0028] The method for using the device and the working principle are as follows: when in use, the cell liquid to be treated is injected into the material holes 22, then the electrode module 3 is buckled on the top surface of the hole plate 2 so that the electrode rods 31 are inserted into the material holes 22, the control module 1 is clamped on the top surface of the electrode module 3 so that the telescopic probes 16 are in pressure contact with the top surface of the conductive rods 34, through the external controller, the electrode rods 31 are electrified through the control circuit 15, the telescopic probes 16, the conductive rods 34 and the circuit board 37, the cell liquid in the material holes 22 is discharged to realize electrolysis operation, through the cooperation of the plurality of material holes 22 and the electrode rods 31, the synchronous electrolysis of a plurality of groups of cell liquid can be realized, through the arrangement of the conductive rod one 311 and the conductive rod two 312, the circuit board 37 is arranged to electrify the two electrode sheets 313 through the conductive rod one 311 and the conductive rod two 312, so that the cell liquid in the communication groove 314 is discharged to realize electrolysis, through the arrangement of the electrode rods 31, the conductive rods 34 and the circuit board 37, the device can realize thirty-two control partitions of ninety-six electrode rods 31, the user can control the partitioned electrolysis of the cell liquid in the material holes 22 in different regions, through the clamping of the buckles 13 and the clamping grooves 35, the control module 1 and the electrode module 3 can be conveniently installed or disassembled, so that the electrode module 3 can be conveniently subjected to high-temperature sterilization treatment after use.
[0029] The above is only a preferred embodiment of the present application, and is not intended to limit the present application in other forms. Any skilled person in the art can modify or change the above disclosed technical content to equivalent embodiments applied to other fields, but any simple modification, equivalent change and modification made on the basis of the technical essence of the present application to the above embodiments still falls within the protection scope of the present application.
Claims
1. A controllable batch cell sample electrical breakdown device, characterized in that: The device includes a control module (1), an orifice plate (2), and an electrode module (3). The control module (1) is snapped onto the top surface of the electrode module (3). The electrode module (3) is movably connected to the top surface of the orifice plate (2). The electrode module (3) includes an electrode rod (31) and an insulating shell (32). The electrode rod (31) is connected through to the bottom surface of the insulating shell (32). A through groove (314) is provided on the outer wall of the electrode rod (31). An installation groove (36) is provided inside the insulating shell (32). A circuit board (37) is fixedly installed on the inner wall of the installation groove (36). A conductive rod (34) is sealed and connected through to the top surface of the insulating shell (32). The bottom end of the conductive rod (34) The control module (1) is connected through the top surface of the circuit board (37). It includes a housing (11). The inner wall of the housing (11) is equipped with a control circuit (15). The bottom surface of the housing (11) is connected through a telescopic probe (16). The control circuit (15) and the telescopic probe (16) are electrically connected. The bottom end of the telescopic probe (16) is pressed against the top end of the conductive rod (34). The perforated plate (2) includes a base plate (21), a material hole (22) and a limiting groove (23). The material hole (22) is opened on the top surface of the base plate (21). The limiting groove (23) is opened on the outer wall of the base plate (21). The electrode rod (31) is inserted into the inside of the material hole (22).
2. The controllable batch cell sample electrical breakdown device according to claim 1, characterized in that: The electrode rod (31) includes a first conductive rod (311), a second conductive rod (312), an electrode sheet (313), and an insulating sleeve (315). The top ends of the first conductive rod (311) and the second conductive rod (312) are inserted into the bottom surface of the circuit board (37). There are two insulating sleeves (315), which are respectively sleeved on the outer walls of the first conductive rod (311) and the second conductive rod (312).
3. The controllable batch cell sample electrical breakdown device according to claim 2, characterized in that: There are two electrode plates (313). The outer walls of the first conductive rod (311) and the second conductive rod (312) are fixedly connected to the outer wall of the electrode plate (313), and the two electrode plates (313) are respectively embedded in the inner wall of the connecting groove (314).
4. The controllable batch cell sample electrical breakdown device according to claim 1, characterized in that: The outer walls at both ends of the outer shell (11) are equipped with buckles (13), and the outer walls at both ends of the insulating shell (32) are provided with slots (35). The bottom end of the buckle (13) is engaged with the inner wall of the slot (35), and the control module (1) is engaged with the electrode module (3) through the buckle (13).
5. The controllable batch cell sample electrical breakdown device according to claim 4, characterized in that: A top cover (14) is fixedly connected to the top surface of the outer shell (11), and an aviation plug (12) is connected through the outer wall of the outer shell (11). The aviation plug (12) is electrically connected to the control circuit (15).
6. The controllable batch cell sample electrical breakdown device according to claim 4, characterized in that: The top surface of the insulating shell (32) is provided with a positioning hole (33), and the bottom surface of the outer shell (11) is fixedly connected with a positioning pin (17), which is inserted into the inner wall of the positioning hole (33).