Lightweight blood analysis device

Abstract

The utility model relates to the technical field of medical instruments, and discloses a lightweight blood analysis device which comprises a detection module and a sample processing module. The sample treatment module comprises a multifunctional liquid suction needle for sucking liquid, a driving device and a dilution module; the multifunctional liquid suction needle is connected with the dilution module through the liquid path pipe, and the driving device controls the multifunctional liquid suction needle to move. The detection module comprises at least one sample position and a built-in reagent position, and the sample position and the built-in reagent position are both located in a moving track area of the multifunctional liquid suction needle. The multifunctional liquid suction needle is not only used for sucking a blood sample and diluting the blood sample for transferring, but also used for sucking and distributing a hemolytic agent and other detection reagents and distributing diluent. According to the design, the device structure and the process are greatly simplified, parts required by the device are greatly reduced, and the device cost and the failure rate are greatly reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical devices, and particularly relates to a light-weight blood analysis device. BACKGROUND

[0002] This part provides only background information related to the present disclosure, which is not necessarily prior art.

[0003] With the continuous progress of medical technology and the improvement of social and economic level, blood analysis as a basic and key clinical examination method has played an increasingly important role in disease diagnosis, treatment monitoring and health management. Blood analysis can provide detailed information about the blood components of patients, including red blood cells, white blood cells, platelets, hemoglobin and other indicators. These information has important clinical value for the diagnosis and treatment of various diseases. For example, through the analysis of white blood cell count and classification, infection, inflammation and immune system abnormalities can be identified; hemoglobin level determination helps to diagnose anemia; platelet count can be used to evaluate hemorrhagic diseases, etc.

[0004] However, in current clinical practice, the existing blood analyzers generally have the problems of complex structure, high equipment cost and detection cost. Blood analyzers usually need to be equipped with multiple complex components to realize automatic analysis of blood samples. The existing blood analysis system mostly needs at least 15 electromagnetic valves, more than one diluter and a complex pipeline system, which not only makes the production cost of the equipment high, but also increases the difficulty of maintenance and management of the equipment, and the failure rate is also relatively high. Due to the involvement of complex reagent proportioning and high-dose consumption, the reagent cost in the detection process is also relatively high, which further increases the overall operation cost of using the equipment.

[0005] Especially in primary medical institutions, due to the relatively small number of patients and low economic benefits, there is often not enough funds to purchase and maintain high-cost blood analysis equipment. At the same time, due to the high failure rate of the equipment and the complex operation, it causes certain difficulties for medical personnel in primary medical institutions to use blood analyzers, which further leads to the fact that the diagnostic effect and clinical value of blood analysis cannot be fully played. Therefore, how to reduce the equipment cost of blood analyzers and improve the stability and reliability of the equipment has become an important issue to improve the level of diagnosis and treatment services in primary medical institutions. SUMMARY

[0006] In view of the deficiencies of the prior art, the present application discloses a light-weight blood analysis device. The blood analysis device has simple structure, low cost, low failure rate and simple operation.

[0007] A light-weight blood analysis device, comprising a sample processing module and a detection module.

[0008] The sample processing module comprises a multifunctional liquid suction needle for sucking liquid, a driving device, and a dilution module for diluting samples; the multifunctional liquid suction needle is connected with the dilution module through a liquid path pipe, and the driving device drives the multifunctional liquid suction needle to move; the multifunctional liquid suction needle cooperates with the dilution module to realize quantitative suction and distribution of reagents, and the multifunctional liquid suction needle has the functions of quantitative suction of samples, dilution of samples, and distribution according to detection needs; the dilution module is connected with an external dilution liquid barrel through a pipe.

[0009] The detection module is used for detection of cell components and hematin of blood samples, and comprises at least one sample site for placing a sample cup and an internal reagent site for placing a detection cup and a reagent bottle, and the sample site and the internal reagent site are located on a movement track of the multifunctional liquid suction needle.

[0010] The multifunctional liquid suction needle can perform movement in more than one dimension, and the multifunctional liquid suction needle performs suction of blood samples from the sample cup into a white blood cell / hematin detection cup and also adds a certain amount of dilution liquid. After the blood samples are mixed in the white blood cell / hematin detection cup, the multifunctional liquid suction needle performs quantitative suction of the diluted and mixed blood samples in the white blood cell / hematin detection cup and transfers them to a red blood cell / platelet detection cup; the multifunctional liquid suction needle also has the function of quantitative suction of hemolytic agent and addition of the hemolytic agent to the white blood cell / hematin detection cup, and the multifunctional liquid suction needle also has the function of quantitative addition of dilution liquid to the white blood cell / hematin detection cup and the red blood cell / platelet detection cup according to the detection and cleaning needs of the device in the device workflow.

[0011] Further, the internal reagent site comprises a hemolytic agent reagent bottle site for providing hemolytic agent and at least two internal reagent sites for placing detection cups; the detection cups are respectively provided with blood detection devices and stirring devices for mixing diluted and treated sample liquid in the cups, and the lower parts of the detection cups are provided with liquid discharge ports, and the detection cups are connected with a waste liquid treatment module through the liquid discharge ports;

[0012] The detection cups are provided with a waste liquid treatment module, the waste liquid treatment module comprises a third liquid flow control valve and a first liquid discharge pump, the detection cups are connected with the third liquid flow control valve through waste liquid discharge pipes, and the third liquid flow control valve is connected with the first liquid discharge pump through a waste liquid discharge collection pipe. The detection cups are arranged in a shielding cover made of a material capable of shielding electromagnetic interference.

[0013] The waste liquid treatment module comprises a third liquid flow control valve and a first liquid discharge pump, the detection cups are respectively connected with the third liquid flow control valve through waste liquid discharge pipes, and the third liquid flow control valve is connected with the first liquid discharge pump through a waste liquid discharge collection pipe. When waste liquid needs to be discharged, the valve is opened, and the waste liquid pump works to discharge the waste liquid in the cup.

[0014] Further, the detection cup and reagent bottle are respectively provided with automatic opening and closing covers, which are opened and closed in cooperation with the movement of the multifunctional liquid suction needle. When the multifunctional liquid suction needle needs to dive into the reagent bottle to suck reagent, or needs to add sample and reagent to the detection cup, the automatic opening and closing covers are automatically opened. When the multifunctional liquid suction needle finishes sucking mixed sample from the detection cup, or finishes adding reagent / diluent to the detection cup, and finishes sucking reagent, the automatic opening and closing covers are automatically closed to avoid the liquid sucked by the multifunctional liquid suction needle from dripping into the lower detection cup or reagent bottle. The automatic opening and closing covers also have the function of preventing reagent carried by the multifunctional liquid suction needle and other related devices from dripping into the detection cup and reagent bottle.

[0015] The material of the automatic opening and closing cover is an external electrical signal interference resistant material. The external electrical signal interference resistant material can be an aluminum foil shielding material. When the detection cup performs detection, the interference of external electromagnetic signals on the detection signal of the device can be avoided, and the quality of the detection result of the device is improved.

[0016] Further, the stirring device includes a magnetic stirring device and a magnetic stirring sub. The magnetic stirring sub is arranged in the detection cup, and the magnetic stirring device is arranged at the bottom outside the detection cup. The magnetic stirring device drives the magnetic stirring sub to move through magnetic force to stir the sample. The bottom of the detection cup is provided with a radial shallow groove strip, which is matched with the shape of the magnetic stirring sub. After the stirring stops, the magnetic stirring sub falls into the radial shallow groove strip, and the stirring sub is in a stable and stationary state when it is not stirring. The movement of the stirring sub during the detection performed by the detection cup can be avoided to interfere with the detection signal. The depth of the radial shallow groove strip is not less than 0.1 mm, and the length of the radial shallow groove strip is not less than the length of the stirring sub.

[0017] The magnetic stirring device is a rotatable magnet. When the magnet below the cup rotates, the stirring sub in the red blood cell / platelet detection cup and the stirring sub in the white blood cell / hematin detection cup are respectively driven to perform mixing of the diluted sample and the sample added with reagent in the cup.

[0018] In some embodiments, the stirring device is an inflation mixing needle arranged in the detection cup and a gas supply pump connected with the inflation mixing needle. The exhaust port of the inflation mixing needle is located in the detection cup, and the gas supply pump provides disturbance gas for the inflation mixing needle to stir and mix the liquid in the detection cup. The gas supply pump inflates the liquid in the red blood cell / platelet detection cup and the white blood cell / hematin detection cup through the exhaust needle to stir and mix the liquid in the cup.

[0019] Further, the dilution module comprises one or more diluters and a diluent tank for placing diluent; the multifunctional liquid suction needle, the first liquid path pipe, the diluter, the second liquid path pipe and the diluent tank are sequentially connected, the first liquid path pipe is provided with a first liquid flow control valve, and the second liquid path pipe is provided with a second liquid flow control valve. The diluent tank can be external or internal; the diluent is quantitatively sucked by the diluter and then sucked into a required device through the multifunctional liquid suction needle.

[0020] When it is required to add diluent into the detection cup through the multifunctional liquid suction needle, the first liquid flow control valve is closed, the second liquid flow control valve is opened, the piston of the diluter is retracted, a negative pressure is formed in the second liquid path pipe to make the diluent in the diluent tank be sucked into the diluter cavity; then the second liquid flow control valve is closed, the first liquid flow control valve is opened, the piston of the diluter is pushed forward, and the sucked diluent in the diluter cavity is then guided to the multifunctional liquid suction needle through the first liquid path pipe and is distributed into the detection cup.

[0021] The dilution module forms a liquid suction and distribution system, and under the cooperative work of the module, the blood analysis device realizes quantitative suction, transfer and dilution of blood samples and suction and distribution of different reagents, including but not limited to diluent and hemolytic agent.

[0022] Further, in some schemes, the dilution module is provided with one or more diluters, and the two diluters are different in size, one large diluter is mainly used for suction and distribution of diluent, and one small diluter is used for suction and distribution of samples and diluted samples, and the suctioned samples and diluted samples are distributed. The two diluters can be driven by two motors respectively or driven by one motor.

[0023] Further, the blood analysis device is provided with an external cleaning device for cleaning the multifunctional liquid suction needle. After the multifunctional liquid suction needle sucks blood samples and reagents, the external cleaning device of the needle is used to clean the blood samples and reagents remaining on the outer surface of the needle.

[0024] The specific structure of the external cleaning device is an annular flow cleaning pool arranged outside the multifunctional liquid suction needle, one water inlet end is arranged at one end of the cleaning pool, and one water outlet end is arranged at the other end of the pool, a diluter is used for water supply of the water inlet end, the diluter pipeline is respectively connected with one three-way valve or two one-way valves, or can be connected with one water supply pump; the water outlet end is connected with a waste liquid pump or a special pump. The multifunctional liquid suction needle is arranged and installed to pass through the center of the annular flow cleaning pool up and down, when the multifunctional liquid suction needle sucks blood samples and reagents, the needle tip drops into the sample cup and the reagent cup, after the sample and the reagent are sucked, the multifunctional liquid suction needle rises and passes through the annular flow cleaning pool in sections, at this time, the diluter of the water supply end of the annular flow cleaning pool starts to supply water, and the waste liquid pump or the special pump of the water discharge end discharges water, so that the outer surface of the needle is cleaned, especially the needle tip retracts into the annular flow cleaning pool, and the cleaning ends.

[0025] Further, the detection cup comprises a white blood cell / hematin detection cup and a red blood cell / platelet detection cup, the white blood cell / hematin detection cup is provided with a second detection device for detecting white blood cells, and the white blood cell / hematin detection cup is provided with a hematin detection device; the red blood cell / platelet detection cup is provided with a first detection device for detecting red blood cells and platelets.

[0026] The white blood cell / hematin detection cup performs one dilution on the blood sample and then adds a hemolytic agent to the cup to dissolve the red blood cells in the diluted blood sample, and the treated mixed solution performs white blood cell / hematin detection after the addition of the hemolytic agent in the cup; and after the white blood cell / hematin detection is completed by adding the hemolytic agent to complete the white blood cell and hematin detection, other reagents can be taken from the reagent bottle by the multifunctional suction needle and added to the cup for mixing to perform other blood component detection. A detection light source is arranged in the white blood cell / hematin detection cup, and a detector is arranged opposite the light source, which is specially used for blood hematin detection in the blood sample.

[0027] The red blood cell / platelet detection cup is used to perform red blood cell and platelet detection after the blood sample is diluted again and mixed in the white blood cell / hematin detection cup.

[0028] Further, the blood analysis device is provided with an optical detection site specially used for performing transmission and scattering detection.

[0029] The optical detection site is located on the movement track of the multifunctional suction needle.

[0030] The optical detection site is a square columnar groove with an open upper part, the inner side wall of the columnar groove is provided with a detection light source and a light signal detector, and the detection light source and the light signal detector are arranged at the same height of the opposite faces or at the same height of the adjacent faces.

[0031] A working process of a light-weight blood analysis device, characterized by comprising the following steps:

[0032] The detection cup comprises a white blood cell / hematin detection cup and a red blood cell / platelet detection cup.

[0033] Step 1: After the operator mixes the blood sample in the sample cup and prepares it, the blood analysis device starts to run, and the sample cup is stretched to the sample position where the multifunctional suction needle probes, and the multifunctional suction needle is inserted into the blood sample in the sample cup; at the same time, the discharge ports of the white blood cell / hematin detection cup and the red blood cell / platelet detection cup are opened, and the first discharge pump is used to discharge the dilution liquid originally stored in the white blood cell / hematin detection cup and the red blood cell / platelet detection cup.

[0034] Subsequently, the drain port control valve of the white blood cell / hemoglobin detection cup and the red blood cell / platelet detection cup is closed, and the first drain pump stops working; after the multifunctional suction needle sucks the blood sample under the cooperation of the dilution module, the sucked blood sample is first injected into the white blood cell / hemoglobin detection cup, and a certain amount of diluent is added to the white blood cell / hemoglobin detection cup through the multifunctional suction needle cooperating with the dilution module; the stirring device arranged in the white blood cell / hemoglobin detection cup mixes the blood sample and the diluent; after mixing, the multifunctional suction needle is inserted into the diluted blood sample in the white blood cell / hemoglobin detection cup, and a certain amount of mixed diluted sample is sucked and transferred to the red blood cell / platelet detection cup by the multifunctional suction needle cooperating with the dilution module; the stirring device arranged in the red blood cell / platelet detection cup mixes the blood sample and the diluent; and the first detection device performs red blood cell and platelet detection on the diluted mixed blood sample in the red blood cell / platelet detection cup.

[0035] The multifunctional suction needle cooperates with the connected diluter, control valve and the like to complete various functions in the above-mentioned and device working processes.

[0036] The mixing of the blood sample and the reagent in the red blood cell / platelet detection cup and the white blood cell / hemoglobin detection cup of the device is performed by a magnetic stirring device or an air stirring mixing device.

[0037] Further, the dilution module of the device can be one diluter or two diluters, and whether it is one or two diluters, they are all in communication with the multifunctional suction needle, and the sample suction and distribution, the built-in reagent suction and distribution, and the diluent suction and distribution are realized by the cooperation of the control valve arranged on the pipeline.

[0038] Further, the dilution module of the device can be two diluters driven by one motor, and the volumes of the two diluters can be the same size or different sizes; when the diluter is of different sizes, the diluter with smaller capacity is used to suck and distribute the blood sample or the diluted blood sample, and is also used to suck and distribute the reagent arranged in the device; and the diluter with larger capacity is used to suck and distribute the diluent arranged outside the device and to distribute the sucked diluent.

[0039] Step 2, under the cooperation of the dilution module and the multifunctional pipette, the multifunctional pipette sucks a certain amount of hemolytic agent from the hemolytic agent bottle and adds it into the white blood cell / hemoglobin detection cup, or adds a certain amount of diluent for further dilution of the diluted blood sample in the white blood cell / hemoglobin detection cup and cleaning of the residual reagent in the needle. The stirring device of the white blood cell / hemoglobin detection cup mixes the newly added hemolytic agent and the diluted blood sample, and under the action of the hemolytic agent, the red blood cells are dissolved and the white blood cells are preserved. The second detection device and the hemoglobin detection device perform white blood cell and hemoglobin detection on the diluted blood sample in the white blood cell / hemoglobin detection cup.

[0040] Step 3, detection data processing, the white blood cell / hemoglobin detection cup and the red blood cell / platelet detection cup respectively obtain white blood cell, hemoglobin, red blood cell and platelet detection data.

[0041] Step 4, waste liquid treatment, after the above detection is completed, the device automatically opens the liquid outlet of the white blood cell / hemoglobin detection cup and the red blood cell / platelet detection cup, and the first liquid pump works to discharge the waste liquid after detection in the white blood cell / hemoglobin detection cup and the red blood cell / platelet detection cup.

[0042] Step 5, cleaning the detection cup, the operator controls the multifunctional pipette to add a certain amount of diluent to the white blood cell / hemoglobin detection cup and the red blood cell / platelet detection cup through the dilution module, and the stirring device stirs and rinses the white blood cell / hemoglobin detection cup and the red blood cell / platelet detection cup. After sufficient rinsing, the device automatically opens the liquid outlet of the white blood cell / hemoglobin detection cup and the red blood cell / platelet detection cup, and the first liquid pump works to discharge the waste liquid after cleaning in the white blood cell / hemoglobin detection cup and the red blood cell / platelet detection cup.

[0043] Step 6, preparation for detection, the operator controls the multifunctional pipette to add a certain amount of diluent to the white blood cell / hemoglobin detection cup and the red blood cell / platelet detection cup and keep it in the cup.

[0044] Step 7, the blood analysis device repeats the detection of different blood samples according to the above steps 1-6, and obtains the white blood cell, hemoglobin, red blood cell and platelet detection result information of each blood sample.

[0045] Further, in some embodiments, the sample cup is a double cup provided with two independent sample storage areas, and each sample storage area is a cup-shaped structure with an upward opening. The first sample storage area is a blood sample cup for collecting and loading raw blood, and the second sample storage area is a pre-dilution cup for pre-dilution of the sample.

[0046] The sample is pre-diluted before step 1. The operator places the sample cup with the collected blood sample on the sample site of the blood analysis device, and then controls the blood analysis device to perform detection. At this time, the multifunctional suction needle enters the sample cup to suck a certain amount of blood sample, and then adds the sucked blood sample into the pre-dilution cup of the sample cup. The multifunctional suction needle adds a certain amount of diluent to the pre-dilution cup through the dilution module, and mixes the blood sample with the added diluent. The blood analysis device performs subsequent blood detection and analysis on the blood sample in the pre-dilution cup according to the use steps of the double-cup device.

[0047] The device drainage pump also participates in the detection function implementation. The cell particle detection device arranged in the detection cup adopts the Coulter electrical resistance principle. An electrode is arranged on the inner and outer sides of the detection cup, and a small detection hole is arranged between the two electrodes. The small detection hole is connected to the outside of the detection cup. The small detection hole is provided with an electrode, and the small detection hole is also connected to a pipeline arranged outside the detection cup and connected to the waste liquid pump. Under the negative pressure drive of the drainage pump during detection, the mixed dilution blood sample in the detection cup flows out of the cup under the negative pressure drive. When the dilution blood sample flows, the different sizes of cell particles pass through the small detection hole. Since the diluent is a constant conductive liquid, the electrical resistance between the inner and outer electrodes changes when the cell particles pass through the small detection hole. The change in electrical resistance is related to the size of the cell particles. The device classifies blood cells according to the size of the cell particles. The number of times of change in electrical resistance is related to the number of cell particles. Therefore, the two detection cups respectively complete the detection of white blood cells, red blood cells and platelets in the blood sample. The negative pressure for attracting the dilution blood sample in the detection cup to flow from the inside to the outside of the detection cup is achieved by pre-abstracting negative pressure from the negative pressure tank arranged on the pipeline by the drainage pump. During detection, the negative pressure is released by the negative pressure tank to attract the dilution mixed blood sample in the detection cup to flow out to complete the detection of blood cells.

[0048] Further, the detection cup of the blood analysis device is arranged in a shielding cover made of metal or other materials with electromagnetic interference shielding function. The shielding cover has an opening above each detection cup, and is provided with an automatic cover opening and closing device. The automatic cover opening and closing device is closed when each detection cup performs detection, to prevent external electromagnetic signal interference. When it is necessary to suck and distribute samples and reagents, the automatic cover opening and closing device is opened. The automatic cover opening and closing device is made of a material that can prevent electromagnetic signal interference, so that the device can avoid external electromagnetic interference during detection.

[0049] Further, the openings of the built-in reagent bottles in the blood analysis device are arranged on the track of the multifunctional suction needle. There is one or more built-in reagent sites, and each built-in reagent site is respectively or uniformly provided with an automatic cover opening and closing device. Only when the multifunctional suction needle needs to suck reagents, the reagent bottle cover is opened, otherwise the reagent cover is closed.

[0050] Further, the blood analysis device is equipped with a special cleaning agent, which is taken and distributed by the multifunctional liquid suction needle.

[0051] Further, in some embodiments, the device is also provided with a colorimetric detection cup, which performs colorimetric detection on blood samples or hemolyzed blood samples, and the samples and reagents used in the colorimetric detection cup are taken and distributed by the multifunctional liquid suction needle from the reagent bottle to the colorimetric detection cup for detection.

[0052] Further, the blood analysis device can use a white blood cell / hemoglobin detection cup to perform detection of other blood components in addition to white blood cells and hemoglobin, such as detection of glycosylated hemoglobin, C-reactive protein, etc. by immunoturbidimetry, and the multifunctional liquid suction needle takes reagents from other reagent bottles and adds them to the white blood cell / hemoglobin detection cup after hemolysis to perform reaction and detection.

[0053] Further, in some embodiments, the blood analysis device is provided with an optical detection site for performing transmission and scattering detection.

[0054] The optical detection site is also arranged on the moving track of the multifunctional liquid suction needle, and is used for biochemical and immunological detection of the whole blood sample.

[0055] The optical detection site is a square or rectangular columnar groove with an open upper part, and detection light sources and light signal detectors are arranged on the inner side wall of the columnar groove, which are arranged at the same height on opposite faces or adjacent faces.

[0056] Further, the tip of the multifunctional liquid suction needle of the blood analysis device is also provided with a conventional slidable external cleaning device, which can clean the residual samples and reagents on the outside of the needle in time during the operation of the device, and ensure that there is no or little residual pollution inside and outside the needle.

[0057] Further, the liquid flow control valve arranged on the pipeline of the blood analysis device can be a three-way valve or a two-way valve, which is used in cooperation with the diluter and the first liquid discharge pump to control the flow direction and flow rate of liquid and gas.

[0058] Advantages:

[0059] The light-weight blood analysis device designed in the application is composed of simpler components, which realizes the blood detection function that originally requires more complex structures and more components.

[0060] The device can complete the transfer and dilution of multiple blood samples by using a multifunctional liquid suction needle, and can also directly take and distribute reagents to perform blood analysis and detection, so the device has a simple workflow, fewer components, and a reduced risk of failure.

[0061] Compared with the existing blood analysis device, the blood sample residue in the internal sample needle needs to be cleaned by using a special diluent, and the device of the present application uses the diluent for diluting the blood sample to clean the multifunctional liquid suction needle, so that the reagent is fully utilized and the reagent consumption is greatly reduced.

[0062] In summary, the blood analysis device designed in the present application has the advantages of simple structure, reduced device cost, reduced operation cost, and reduced failure rate, and can better realize the popularization of blood analysis devices in primary medical institutions, and is beneficial to help primary medical institutions improve service quality and service level. BRIEF DESCRIPTION OF DRAWINGS

[0063] The above and / or other aspects of the present application will become apparent from the following detailed description of the application taken in conjunction with the accompanying drawings.

[0064] Figure 1 The main structural relationship diagram of the device designed in the present application is shown.

[0065] Figure 2 The related structural diagram of the device designed in the present application when equipped with two diluters is shown.

[0066] Figure 3 The top view of the double special sample cup designed in the present application is shown.

[0067] Figure 4 The longitudinal sectional top view of the double special sample cup designed in the present application is shown. DETAILED DESCRIPTION

[0068] The reference signs in the drawings are as follows: 1-sample cup; 2-multifunctional liquid suction needle; 3-fixed driving device; 4-moving slide rail; 5-first liquid path pipe; 6-white blood cell / hematin detection cup; 7-red blood cell / platelet detection cup; 8-hemolytic agent reagent bottle; 9-second liquid path pipe; 10-diluent barrel; 11-diluter; 12-first detection device; 13-second detection device; 14-magnetic stirring device; 15-magnetic stirring sub; 16-waste liquid discharge pipe; 17-waste liquid discharge collection pipe, 18-first liquid flow control valve; 19-second liquid flow control valve; 20-third liquid flow control valve; 21-first liquid discharge pump; 22-fourth liquid flow control valve; 23-second diluter; 24-fifth liquid flow control valve; 25-multifunctional liquid suction needle outer wall cleaning pool; 26-sixth liquid flow control valve; 27-second liquid discharge pump; 28-double cup; 281-first sample storage area; 282-second sample storage area.

[0069] Example 1:

[0070] As Figure 1As shown, a light blood analysis device comprises a detection module, a sample processing module and a detection module. The sample processing module comprises a multifunctional liquid suction needle 2 for sucking liquid, a driving device and a dilution module for diluting samples; the multifunctional liquid suction needle 2 is connected with the dilution module through a liquid path pipe, and the driving device controls the movement of the multifunctional liquid suction needle 2.

[0071] The detection module is used for detecting the cell components of the blood sample and the components contained in the plasma, and comprises at least one sample site for placing a sample cup and at least two built-in reagent sites for placing detection reagents, wherein the built-in reagent sites are located on the movement track of the multifunctional liquid suction needle 2.

[0072] The driving device comprises a fixed driving device 3 for controlling the multifunctional liquid suction needle 2 and a movable sliding rail 4 matched with the fixed driving device 3.

[0073] The built-in reagent site comprises a hemolytic reagent bottle 8 site for providing a hemolytic reagent and at least two built-in reagent sites for placing detection cups, wherein the detection cups are provided with a blood detection device and a stirring device for stirring samples, the detection cups are provided with an automatic cover opening and closing device, the lower part of the detection cups is provided with a liquid discharge port, the detection cups are connected with a waste liquid processing module through the liquid discharge port, and the detection cups are arranged in a shielding cover made of a material capable of shielding electromagnetic interference.

[0074] The detection cup is provided with a waste liquid processing module, and the waste liquid processing module comprises a third liquid flow control valve 20 and a first liquid discharge pump 21, the detection cup is connected with the third liquid flow control valve 20 through a waste liquid discharge pipe 16, and the third liquid flow control valve 20 is connected with the first liquid discharge pump 21 through a waste liquid discharge collection pipe 17. The third liquid flow control valve 20 can simultaneously close or open two 16 waste liquid discharge channels.

[0075] The stirring device comprises a magnetic stirring device 14 and a magnetic stirring sub 15, the magnetic stirring sub 15 is arranged in the detection cup, the magnetic stirring device 14 is arranged at the bottom outside the detection cup, the magnetic stirring device 14 drives the magnetic stirring sub 15 to move through magnetic force to stir the sample, and the inner bottom of the detection cup is provided with a radial shallow groove strip, the radial shallow groove strip is matched with the shape of the magnetic stirring sub 15, and the magnetic stirring sub 15 falls into the radial shallow groove strip after the stirring stops.

[0076] The dilution module comprises a diluter 11 and a dilution liquid barrel 10 for placing dilution liquid; the multifunctional liquid suction needle 2, the first liquid path pipe 5, the diluter 11, the second liquid path pipe 9 and the dilution liquid barrel 10 are connected in sequence, the first liquid path pipe 5 is provided with a first liquid flow control valve 18, and the second liquid path pipe 9 is provided with a second liquid flow control valve 19.

[0077] When it is necessary to add diluent into the detection cup through the multi-functional suction needle 2, the first liquid flow control valve 18 is closed, the second liquid flow control valve 19 is opened, the piston of the diluter 11 is retracted, and a negative pressure is formed in the second liquid path pipe 9 so that the diluent in the diluent tank 10 is sucked into the diluter cavity; then the second liquid flow control valve 19 is closed, the first liquid flow control valve 18 is opened, the piston of the diluter 11 is pushed forward, and the diluent sucked in the diluter 11 cavity is then passed through the first liquid path pipe 5 to the multi-functional suction needle 2 and is distributed into the detection cup.

[0078] The blood analysis device is provided with a cleaning tank for cleaning the multi-functional suction needle 2.

[0079] The detection cup includes a leukocyte / hematin detection cup 6 provided with the second detection device 13 and a red blood cell / platelet detection cup 7 provided with the first detection device 12.

[0080] As shown in Figures 3-4 The sample cup 1 is a double cup 28 provided with two independent sample storage areas, each of which is a cup-shaped structure with an upward opening. The first sample storage area 281 is a blood sample cup for collecting and loading raw blood, and the second sample storage area 282 is a pre-dilution cup for performing the first pre-dilution on the sample.

[0081] The working process of the light-weight blood analysis device includes the following steps:

[0082] Step 1: After the operator mixes the blood sample in the sample cup 1 and prepares it, the operator extends the sample cup 1 to the aspiration position of the multi-functional suction needle 2, and the multi-functional suction needle 2 is inserted into the blood sample in the sample cup 1; at the same time, the discharge port of the leukocyte / hematin detection cup 6 and the red blood cell / platelet detection cup 7 is opened, and the original diluent in the leukocyte / hematin detection cup 6 and the red blood cell / platelet detection cup 7 is discharged through the first discharge pump 21.

[0083] Subsequently, the drain valve of the leukocyte / hemochrome detection cup 6 and the red blood cell / platelet detection cup 7 is closed, and the first drain pump 21 stops working; after the multi-functional suction needle 2 sucks the blood sample under the cooperation of the dilution module, the sucked blood sample is first injected into the leukocyte / hemochrome detection cup 6, and a certain amount of diluent is added to the leukocyte / hemochrome detection cup 6 through the multi-functional suction needle 2 cooperating with the dilution module; the stirring device arranged in the leukocyte / hemochrome detection cup 6 mixes the added blood sample and diluent, and after mixing, the multi-functional suction needle 2 is inserted into the diluted blood sample in the leukocyte / hemochrome detection cup 6, and a certain amount of mixed diluted sample is sucked and transferred to the red blood cell / platelet detection cup 7 by the multi-functional suction needle 2 cooperating with the dilution module; a certain amount of diluent is added to the red blood cell / platelet detection cup 7 by the multi-functional suction needle 2 cooperating with the dilution module; the stirring device arranged in the red blood cell / platelet detection cup 7 mixes the added blood sample and diluent, and the first detection device 12 performs red blood cell and platelet detection on the diluted and mixed blood sample in the red blood cell / platelet detection cup 7.

[0084] Step 2, under the cooperation of the dilution module and the multi-functional suction needle 2, the multi-functional suction needle 2 sucks a certain amount of hemolytic agent from the hemolytic agent bottle 8 and adds it to the leukocyte / hemochrome detection cup 6, or adds a certain amount of diluent for further dilution of the diluted blood sample in the leukocyte / hemochrome detection cup 6 and cleaning of the residual reagent in the needle; the stirring device of the leukocyte / hemochrome detection cup 6 mixes the newly added hemolytic agent and the diluted blood sample, and under the action of the hemolytic agent, the red blood cells are dissolved and the white blood cells are preserved; the second detection device 13 and the hemochrome detection device perform leukocyte and hemochrome detection on the diluted blood sample in the leukocyte / hemochrome detection cup 6.

[0085] Step 3, detection data processing, the leukocyte / hemochrome detection cup 6 and the red blood cell / platelet detection cup 7 respectively obtain leukocyte, hemochrome, red blood cell and platelet detection data.

[0086] Step 4, waste liquid treatment, the drain valve of the leukocyte / hemochrome detection cup 6 and the red blood cell / platelet detection cup 7 is opened, and the first drain pump 21 works to drain the waste liquid in the leukocyte / hemochrome detection cup 6 and the red blood cell / platelet detection cup 7.

[0087] Step 5, cleaning the detection cup, the operator controls the multi-functional suction needle 2 to add a certain amount of diluent into the white blood cell / hematin detection cup 6 and the red blood cell / platelet detection cup 7 through the dilution module, the stirring device rinses the white blood cell / hematin detection cup 6 and the red blood cell / platelet detection cup 7, and the liquid in the white blood cell / hematin detection cup 6 and the red blood cell / platelet detection cup 7 is discharged after sufficient rinsing. The third liquid flow control valve 20 is opened again, and the first liquid discharge pump 21 is opened again to work to empty the liquid in the two cups; the device can also execute the above cleaning process on the two cups more than once.

[0088] Step 6, preparation for detection, the operator controls the multi-functional suction needle 2 to add a certain amount of diluent into the white blood cell / hematin detection cup 6 and the red blood cell / platelet detection cup 7 and keep it in the cup. The device can enter the detection of the next sample.

[0089] Step 7, the blood analysis device repeats the detection of different blood samples according to the above steps 1-6 to obtain the white blood cell, hematin, red blood cell and platelet detection result information of each blood sample.

[0090] The device can also pre-dilute the sample before step 1. The operator places the sample cup 1 (as shown in Figures 3-4 ) containing the collected blood sample on the sample position of the blood analysis device and controls the blood analysis device. The multi-functional suction needle 2 enters the blood sample cup of the sample cup 1 to suck a certain amount of blood sample, and then adds the sucked blood sample into the pre-dilution cup of the sample cup 1. The multi-functional suction needle 2 adds a certain amount of diluent into the pre-dilution cup through the dilution module and mixes the diluent and the blood sample. The blood analysis device performs subsequent blood analysis on the blood sample in the pre-dilution cup.

[0091] As shown in Figure 2 , in some embodiments, the dilution module of the device has two diluters, i.e. the diluter 11 and the second diluter 23. The diluter 11 has a larger capacity and is mainly used for sucking and dispensing diluent, while the second diluter 23 has a smaller capacity and is mainly used for sucking and dispensing blood sample and diluted blood sample. The two diluters can be driven by one motor or driven by two different motors.

[0092] The tip of the multi-functional suction needle of the device is also provided with a needle outer surface cleaning device 25. The specific structure of the device is an annular flow cleaning pool arranged outside the multi-functional suction needle.

[0093] The sample, built-in reagent suction and distribution, and diluent suction and distribution workflow of the blood analysis device in the design when loading two diluters is as follows: when the multifunctional suction needle needs to suck the sample or the built-in reagent, or the sample needs to be diluted and mixed, the first liquid flow control valve 18 is closed, the fifth liquid flow control valve 24 is opened, and the piston of the second diluter 23 is retracted to suck the sample, dilute the sample, or the built-in reagent; when the sample or the reagent sucked by the multifunctional suction needle 2 needs to be distributed, the piston of the second diluter 23 is pushed forward, and the sample sucked by the multifunctional suction needle 2 is pushed out.

[0094] When the diluent needs to be sucked and distributed, first, the first liquid flow control valve 18 and the fourth liquid flow control valve 22 are closed, the second liquid flow control valve 19 is opened, the piston of the diluter 11 is retracted, and the diluent is sucked into the inner cavity of the diluter 11 due to the negative pressure formed by the retraction of the diluter 11. Then, the second liquid flow control valve 19 is closed, the first liquid flow control valve 18 and the fifth liquid flow control valve 24 are opened, the piston of the diluter 11 is pushed forward, a larger positive pressure is formed in the diluter 11 to drive the diluent in the inner cavity of the diluter 11 to flow through the opened first liquid flow control valve 18 and fifth liquid flow control valve 24, and then be distributed to the target container through the multifunctional suction needle 2.

[0095] When the multifunctional suction needle 2 needs to be cleaned, first, the first liquid flow control valve 18 and the fourth liquid flow control valve 22 are closed, the second liquid flow control valve 19 is opened, the piston of the diluter 11 is retracted to form a negative pressure in the diluter 11, and the diluent in the diluent barrel 10 is sucked into the inner cavity of the diluter 11 through the pipeline. Then, the second liquid flow control valve 19 is closed, the fourth liquid flow control valve 22 is opened, the piston of the diluter 11 is pushed forward, the diluent in the inner cavity of the diluter 11 is driven by the pressure to flow to the water inlet of the multifunctional suction needle outer surface cleaning device 25 through the pipeline connected by the fourth liquid flow control valve 22. At the same time, the third liquid flow control valve 20 is closed, the sixth liquid flow control valve 26 is opened, and the second liquid pump 27 is started to work, so that the diluent in the multifunctional suction needle outer surface cleaning device 25 is quickly discharged to clean the outer surface of the multifunctional suction needle.

[0096] Example 2:

[0097] The difference between Example 2 and Example 1 is that the blood analysis device uses different stirring devices.

[0098] The stirring device includes a gas mixing needle arranged in the measuring cup and a gas supply pump connected with the gas mixing needle. The exhaust port of the gas mixing needle is located in the measuring cup, and the gas supply pump provides disturbance gas for the gas mixing needle.

[0099] When the dilute blood sample in the cup needs to be stirred, the air pressure pump at the other end of the air mixing needle is connected and works, pressurizing the exhaust passage, and the gas enters the liquid in each cup through the exhaust passage, stirring the dilute blood sample liquid to achieve the effect of stirring the dilute blood.

[0100] Embodiment 3:

[0101] Embodiment 3 is different from the above embodiments in that the detection cup of the blood analysis device comprises a colorimetric detection cup.

[0102] The blood analysis device is provided with a colorimetric detection cup for performing colorimetric detection of blood samples or hemolyzed blood samples, and the samples and reagents used in the colorimetric detection cup are all taken and distributed into the colorimetric detection cup by the multifunctional pipette for detection.

[0103] When the device performs detection, the multifunctional pipette of the device can also take a certain amount of treated, diluted and mixed blood sample from the leukocyte / hematin detection cup 6 with hemolysin added, add it to the colorimetric cell, and also take a certain amount of reagent from one or more reagent bottles and add it to the colorimetric cell, to realize detection of other biochemical items of the blood sample, such as glycated hemoglobin, C-reactive protein detection, etc.

[0104] The colorimetric detection cup can also be used for pre-dilution of the blood sample.

[0105] Embodiment 4:

[0106] Embodiment 4 is different from the above embodiments in that the blood analysis device is provided with an optical detection site specially used for performing transmission and scattering detection.

[0107] The optical detection site is also arranged on the moving track of the multifunctional pipette, and is used for detecting biochemical, immune and other material components of the whole blood sample.

[0108] The optical detection site is a square or rectangular columnar groove with an open upper part, and detection light sources and light signal detectors are arranged on the inner side wall of the columnar groove. The detection light sources and light signal detectors are arranged at the same height on opposite faces, or at the same height on adjacent faces.

[0109] The detection process is performed in the following manner:

[0110] The detection cup pre-loaded with specific reagent is put into the optical detection position before the detection is performed. When the device performs the detection, the multifunctional pipette first sucks a certain amount of sample from the sample tube, and distributes the sample into a pre-dilution cup. A certain amount of diluent is injected from the multifunctional pipette into the pre-dilution cup. The multifunctional pipette also performs the suction and mixing of the diluted blood sample in the pre-dilution cup. The blood sample is diluted in the pre-dilution cup by a low dilution factor of no more than 10 times. Then, the multifunctional pipette sucks a certain amount of blood sample from the pre-dilution cup and adds it into the leukocyte / hematin detection cup. The diluted sample is mixed and uniformly reacted according to the flow chart to complete the detection.

[0111] Before or after the detection, the multifunctional pipette sucks a certain amount of diluted blood sample from the pre-dilution cup and adds it into the detection cup placed in the optical detection position. The sample is mixed and reacted with the reagent in the detection cup. The detector arranged in the detection position detects the change in absorbance value of the mixed and reacted sample and reagent, and obtains the detection result of the components in the specific blood sample.

[0112] The present application provides a light weight blood analysis device design idea and method. There are many methods and approaches to realize the technical solution. The above description is only the preferred embodiment of the present application design. It should be pointed out that for ordinary skilled in the art, without departing from the principle of the present application design, some improvements and refinements can be made. These improvements and refinements should also be considered within the protection scope of the present application design. The components not explicitly described in the embodiment can be realized by using existing technology.

Claims

1. A lightweight blood analysis device, characterized by, The device comprises a sample processing module and a detection module. The sample processing module comprises a multifunctional liquid suction needle (2) for sucking liquid, a driving device and a dilution module for diluting samples; the multifunctional liquid suction needle (2) is connected with the dilution module through a liquid path pipe, the driving device drives the multifunctional liquid suction needle (2) to move; the dilution module is connected with an external dilution liquid barrel (10) through a pipe. The detection module comprises at least one sample site for placing a sample cup and an internal reagent site for placing a detection cup and a reagent bottle, and the sample site and the internal reagent site are located on the movement track of the multifunctional liquid suction needle (2).

2. The lightweight blood analysis device of claim 1, wherein The detection cup is more than one, each detection cup is respectively provided with a blood detection device and a stirring device for mixing the diluted sample liquid in the cup, and the lower part of the detection cup is provided with a liquid discharge port, and the detection cup is connected with a waste liquid processing module through the liquid discharge port. The waste liquid processing module comprises a third liquid flow control valve (20) and a first liquid discharge pump (21), and the detection cup is connected with the third liquid flow control valve (20) through a waste liquid discharge pipe (16), and the third liquid flow control valve (20) is connected with the first liquid discharge pump (21) through a waste liquid discharge collection pipe (17).

3. The lightweight blood analysis device of claim 2, wherein, The upper parts of the detection cup and the reagent bottle are respectively provided with automatic cover opening and closing devices, the automatic cover opening and closing devices are matched with the movement of the multifunctional liquid suction needle (2) to open and close, when the multifunctional liquid suction needle (2) needs to be inserted into the reagent bottle or the detection cup site, the automatic cover opening and closing devices are opened to expose the corresponding reagent bottle or detection cup port, and when the multifunctional liquid suction needle (2) completes the blood sample, reagent suction and distribution, the automatic cover opening and closing devices are closed, and the material of the automatic cover opening and closing devices is an external electric signal interference resistant material.

4. The lightweight blood analysis device of claim 3, wherein The stirring device comprises a magnetic stirring device (14) and a magnetic stirring sub (15), the magnetic stirring sub (15) is arranged in the detection cup, the magnetic stirring device (14) is arranged at the outer bottom of the detection cup, the magnetic stirring device (14) drives the magnetic stirring sub (15) to move through magnetic force to stir the sample; The inner bottom of the detection cup is provided with a radial shallow groove strip, the radial shallow groove strip is matched with the shape of the magnetic stirring sub (15), and the magnetic stirring sub (15) falls into the radial shallow groove strip after stirring stops, so that the stirring sub is in a stable and static state when not stirring.

5. The lightweight blood analysis device of claim 3, wherein The stirring device is a gas mixing needle arranged in each detection cup and a gas supply pump connected with the gas mixing needle, the gas outlet of the gas mixing needle is located in the detection cup, and the gas supply pump provides disturbance gas for the gas mixing needle to stir and mix the liquid in the detection cup.

6. The lightweight blood analysis device according to claim 4 or 5, characterized in that The dilution module comprises a diluter (11) and a dilution liquid barrel (10) arranged outside the device for placing dilution liquid; the multifunctional liquid suction needle (2), the first liquid path pipe (5), the diluter (11), the second liquid path pipe (9) and the dilution liquid barrel (10) are sequentially connected, the first liquid path pipe (5) is provided with a first liquid flow control valve (18), and the second liquid path pipe (9) is provided with a second liquid flow control valve (19).

7. The lightweight blood analysis device of claim 6, wherein The detection cup comprises a leukocyte / hematin detection cup (6) provided with a second detection device (13) for detecting leukocytes, and a red blood cell / platelet detection cup (7) provided with a hematin detection device.

8. The lightweight blood analysis device of claim 7, wherein, The blood analysis device is provided with an optical detection site dedicated to performing transmission and scattering detection; The optical detection site is located on the movement track of the multifunctional suction needle (2); The optical detection site is a square columnar groove with an open upper portion, and the inner side wall of the columnar groove is provided with a detection light source and a light signal detector, and the detection light source and the light signal detector are arranged at the same height of opposite faces or adjacent faces.

9. The lightweight blood analysis device of claim 8, wherein, The device is provided with a sample site for placing a dedicated sample cup (1), and the sample cup is a double cup (28) provided with two independent sample storage areas, and each sample storage area is a cup-shaped structure with an upward opening, wherein the first sample storage area (281) is a blood sample cup for collecting and loading raw blood, and the second sample storage area (282) is a pre-dilution cup for performing the first pre-dilution on the sample.

10. The lightweight blood analysis device of claim 9, wherein, The detection cup comprises a colorimetric detection cup.

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