Experimental rat thrombus preparation device

Abstract

The utility model discloses an experimental rat thrombus preparation device which comprises a rectangular groove used for containing blood, the blood is solidified at the bottom of the rectangular groove, scale marks are arranged on the inner wall of the rectangular groove, and the amount of the blood injected into the rectangular groove is controlled through the scale marks. The top of the rectangular groove is detachably connected with a sealing plate; a cutting block and a square frame are further included, the cutting block comprises a horizontal panel, parallel cutting knives are densely distributed at the bottom of the horizontal panel, the distance between the cutting knives is 1-3 mm, a handle is arranged in the center of the top of the horizontal panel, and the cutting block is used for cutting blood clots solidified at the bottom of the rectangular groove; the front baffle and the side baffles of the square frame are detachably connected, and the square frame is used for being placed in the rectangular groove and abutting against one corner of the rectangular groove so as to guide the cutting block. And the blood is coagulated in the rectangular groove, and the coagulated blood is planar, so that the size of the blood clot can be stably controlled, and the problems that the size of the original capillary glass tube thrombus block is unstable, and the coagulated blood is difficult to take out from the capillary glass tube are solved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the medical experiment equipment technical field, concretely relates to a kind of experimental rat thrombus preparation device. BACKGROUND

[0002] In the cardiovascular internal medicine experiment, need to take rat as experimental object to carry out research, research thromboembolic pulmonary hypertension (CTEPH), the most popular method for building CTEPH rat model is autologous thrombus method at present.Simply speaking: whole process must be kept sterile, simulate the thrombus formed by autology, inject thrombus in vein, cause pulmonary thromboembolism and then form pulmonary arterial hypertension.General operating method is to take the fresh blood of rat from orbital vein with capillary glass tube, place in culture dish, make blood coagulation overnight.Again, use physiological saline to flush out the coagulated long thrombus, and use ophthalmic scissors to form 1-3mm blood clot.

[0003] The problems of current test method are: 1. due to the small pore size of capillary glass tube, the coagulated blood is bonded with the inner wall of glass tube with greater force, and it is difficult to flush the blood clot out of capillary glass tube with saline.2. Thrombus needs to be cut into millimeter unit block, and operator needs to control the size and length, which is difficult to operate and time-consuming and labor-consuming.3. It is difficult to accurately control the size and length of thrombus by artificial operation, if length is too long, intravenous infusion can easily lead to rat death; if length is too small, it is not easy to lead to pulmonary embolism to form pulmonary arterial hypertension model.4. The risk of causing harm to rat increases in the process of taking blood from rat orbital vein, such as too much blood loss of rat caused by improper technique, blindness caused by eyeball damage, etc.The above four points are the disadvantages of capillary glass tube autologous thromboembolic pulmonary arterial hypertension rat model. CONTENT OF UTILITY MODEL

[0004] The utility model provides a kind of experimental rat thrombus preparation device, the purpose is to improve the existing thrombus preparation process, solve the problem that original thrombus is blocked in capillary glass tube, difficult to take out and unable to accurately control the size of thrombus.

[0005] Therefore, the utility model adopts the following technical solutions:

[0006] A kind of experimental rat thrombus preparation device, including the rectangular groove for containing blood, blood coagulates in the bottom of rectangular groove, scale line is equipped on the inner wall of rectangular groove, and the amount of blood injected into rectangular groove is controlled by scale line;The top of rectangular groove is detachably connected with sealing plate;

[0007] It also includes a cutting block and a square frame. The cutting block includes a horizontal panel with a dense array of parallel cutting blades at the bottom, spaced 1-3 mm apart. A handle is located at the center of the top of the horizontal panel. The cutting block is used to cut the coagulated blood clots at the bottom of the rectangular groove. The front and side baffles of the square frame are detachably connected. The square frame is used to be placed inside the rectangular groove and abuts against one corner of the groove to guide the cutting block. Further, the rectangular groove is made of rigid plastic or aluminum alloy.

[0008] Furthermore, the cross-section of the cutting blade is wedge-shaped.

[0009] The beneficial effects of this utility model are as follows:

[0010] 1. Blood coagulates in a rectangular groove, and the coagulated blood is in a planar shape, which can stably control the size of the blood clot. This solves the problems of unstable thrombus size and difficulty in removing coagulated blood from the capillary glass tube, which were previously used in capillary glass tubes.

[0011] 2. Using a cutting block to cut coagulated blood allows for precise control of the size of the thrombus after cutting, facilitating subsequent experiments;

[0012] 3. This device allows for blood collection using a syringe, avoiding the damage to the rat's orbit caused by the original orbital vein blood collection method. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the structure of this utility model;

[0014] In the diagram: 1-rectangular groove, 2-cutting block, 3-handle, 4-scale line, 5-sealing plate, 6-square frame, 7-front baffle, 8-side baffle. Detailed Implementation

[0015] The present invention will be further described below with reference to the accompanying drawings and specific embodiments:

[0016] like Figure 1 As shown, a rat thrombus preparation device includes a rectangular trough 1 for holding blood, which coagulates at the bottom of the trough 1. The inner wall of the rectangular trough 1 has graduations 4, which control the amount of blood injected into the trough 1. The rectangular trough 1 is made of rigid plastic or aluminum alloy. A sealing plate 5 is detachably connected to the top of the rectangular trough 1 via a sliding groove structure. The sealing plate 5 is used to seal the blood in the rectangular trough 1 to reduce the risk of contamination during the coagulation process.

[0017] Also included are cutting block 2 and square frame 6, cutting block 2 includes horizontal panel, the bottom of horizontal panel is densely covered with parallel cutting knives, the interval of cutting knives is 1-3mm, the cross section of cutting knives is wedge-shaped, the center of the top of horizontal panel is provided with handle 3, cutting block 2 is used for cutting blood clot solidified at the bottom of rectangular groove 1. Front baffle 7 and side baffle 8 of square frame 6 are detachably connected, square frame 6 is used for being put into rectangular groove 1 and abutting against a corner of rectangular groove 1 so as to provide guidance for cutting block 2.

[0018] A certain amount of fresh venous blood (mostly tail vein) is taken from rats, the blood is injected into square frame 6 of the device from a syringe, the inner side of square frame 6 is also provided with scale line 4, the amount of blood injected into square frame 6 (thrombus height is generally 1-2mm) is controlled through scale line 4, sealing plate 5 is covered to isolate pollution and keep the blood moist. After overnight blood coagulation, front baffle 7 and side baffle 8 are taken, square thrombus block can be seen; 10-15ml PBS or normal saline is injected into rectangular groove 1, cutting device is used to cut once horizontally, the thrombus is cut and cut once vertically, the thrombus is cut into 1-3mm small thrombus block, and thrombus block PBS / normal saline suspension is prepared. A certain amount of thrombus block suspension is taken each time to form embolism through rat jugular vein, and then CTEPH model is prepared.

Claims

1. A device for preparing thrombi in experimental rats, characterized in that, It comprises a rectangular groove (1) for containing blood, the blood is coagulated at the bottom of the rectangular groove (1), the inner wall of the rectangular groove (1) is provided with a scale line (4) for controlling the amount of blood injected into the rectangular groove (1); the top of the rectangular groove (1) is detachably connected with a sealing plate (5); It also comprises a cutting block (2) and a square frame (6), the cutting block (2) comprises a horizontal panel, the bottom of the horizontal panel is densely covered with parallel cutting knives, the interval of the cutting knives is 1-3mm, the center of the top of the horizontal panel is provided with a handle (3), the cutting block (2) is used for cutting the blood clot coagulated at the bottom of the rectangular groove (1); the front baffle (7) and the side baffle (8) of the square frame (6) are detachably connected, the square frame (6) is used for being put into the rectangular groove (1) and abutting against a corner of the rectangular groove (1) so as to provide a guide for the cutting block (2).

2. The experimental rat thrombus preparation apparatus according to claim 1, characterized by The rectangular groove (1) is made of hard plastic plate or aluminum alloy plate.

3. The experimental rat thrombus preparation apparatus according to claim 1, wherein The cross section of the cutting knife is wedge-shaped.

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