Detection device for rapidly detecting His tag protein

Abstract

The utility model discloses a detection device for rapidly detecting His tag protein, which comprises a card box, a detection device, a detection device and a control device, wherein the two sides of the top of the card box incline inwards; the detection card is sealed in the card box, the top of the detection card abuts against the inward inclined position of the card box, and the width of the card box is matched with that of the detection card; the liquid taking device is connected with the detection card and extends out of the card box; wherein the detection card comprises a PVC bottom plate, a first test strip and a second test strip are symmetrically arranged on the PVC bottom plate left and right, the first test strip and the second test strip respectively comprise a sample pad, a colloidal gold pad, a nitrocellulose membrane and absorbent paper which are sequentially lapped, and the nitrocellulose membrane is provided with a detection strip and a quality control strip. The kit is simple in shape, reasonable in structure, high in detection speed, high in sensitivity, high in specificity, convenient to use, low in manufacturing cost, suitable for large-scale popularization and application, and especially suitable for rapid detection of His tag protein.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of protein detection, especially to a detection device for rapid detection of His-tagged protein. BACKGROUND

[0002] At present, His is widely used as a protein tag, especially in the field of biological and medical research. However, most research institutions do not need to produce His protein in large quantities like protein production companies, and they also do not have large-scale professional protein production and quality control equipment. After the research institutions produce His-tagged protein in small quantities, they use the commonly used BCA method protein concentration determination kit on the market for detection. However, due to the interference of proteins in the protein production medium, it is impossible to determine whether the His-tagged protein is successfully produced. Moreover, the current method for detecting His-tagged protein requires a large amount of sample, and there are problems such as time-consuming, labor-intensive, and high cost.

[0003] Therefore, the present application aims to develop a detection device for rapid detection of His-tagged protein, which requires less sample amount, is simple and fast, does not require special instruments and equipment, and can be used in laboratories, supermarkets, homes, and workshops. CONTENT OF THE UTILITY MODEL

[0004] The technical problem to be solved by the present application is that there is a lack of a device for rapidly and effectively detecting His-tagged protein in the prior art, which is suitable for small-scale production of His-tagged protein by research institutions and the like.

[0005] To solve the above technical problems, the present application provides a detection device for rapid detection of His-tagged protein,

[0006] The detection device comprises a cartridge, two sides of the top of which are inwardly inclined; a detection card, which is sealed in the cartridge and abuts against the inwardly inclined part of the top of the cartridge, the width of the cartridge being matched with the width of the detection card; and a liquid taking device, which is connected to the detection card and extends out of the cartridge. The detection card comprises a PVC bottom plate, on which a first test strip and a second test strip are symmetrically arranged. The first test strip and the second test strip each comprise a sample pad, a colloidal gold pad, a nitrocellulose membrane, and an absorbent paper, which are sequentially connected. The nitrocellulose membrane is provided with a detection strip and a quality control strip. The colloidal gold pad is coated with microspheres of a first anti-His-tagged protein antibody. The detection strip of the first test strip is coated with microspheres of a second anti-His-tagged protein antibody. The detection strip of the second test strip is coated with microspheres of a third anti-His-tagged protein antibody. The quality control strip is coated with microspheres of a goat anti-mouse IgG polyclonal antibody.

[0007] The detection device for rapid detection of His-tagged protein, wherein the liquid taking device comprises a sampling pad and a protective shell. The sampling pad is connected to the sample pad, and the part of the sampling pad extending out of the cartridge is sealed in the protective shell.

[0008] The detection device for rapidly detecting His-tag proteins, wherein the upper portion of the protective shell is provided with a folding line, and the protective shell is folded along the folding line to expose the sampling pad.

[0009] The detection device for rapidly detecting His-tag proteins, wherein the bottom of the sampling pad is inverted conical, and the protective shell is cylindrical.

[0010] The detection device for rapidly detecting His-tag proteins, wherein the card box is a box body made of transparent plastic.

[0011] The detection device for rapidly detecting His-tag proteins, wherein the two sides of the nitrocellulose membrane are respectively overlapped below the colloidal gold pad and the absorbent paper.

[0012] The detection device for rapidly detecting His-tag proteins, wherein the card box is rectangular as a whole.

[0013] The detection device for rapidly detecting His-tag proteins, wherein the first test strip and the second test strip are fixed on the PVC bottom plate in a pasting manner.

[0014] The detection device for rapidly detecting His-tag proteins, wherein the detection strip is located on one side of the nitrocellulose membrane close to the colloidal gold pad, and the quality control strip is located on the other side of the nitrocellulose membrane away from the colloidal gold pad.

[0015] The detection device for rapidly detecting His-tag proteins, wherein the material of the microspheres is polystyrene.

[0016] Compared with the prior art, the technical scheme of the present application has the following beneficial effects:

[0017] 1. The present application combines colloidal black gold technology and protein specific reaction, and can rapidly detect His-tag proteins in the detection sample according to the test line and the quality control line of the detection card sample, the detection device is convenient to use, rapid in detection, high in sensitivity and accurate in result.

[0018] 2. The detection device of the present application contains two test strips, and can detect the sample twice at a time, greatly increasing the accuracy of the detection result.

[0019] 3. The card box of the present application is made of transparent plastic, the entire detection process and detection result can be directly observed through the card box, without the need for additional observation port, reducing the manufacturing process and time of the card box, and the overall strength is better. BRIEF DESCRIPTION OF DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings of the embodiments will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application and not limit the present application.

[0021] Figure 1 Structure schematic view of the utility model example;

[0022] Figure 2 Detection card structure schematic view of the utility model example;

[0023] Figure 3 Side view of Figure 2

[0024] The following is the explanation of the reference signs:

[0025] 10. card box, 20. detection card, 21. first test strip, 22. second test strip, 23. quality control strip, 24. absorbent paper, 25. sample pad, 26. colloidal gold pad, 27. nitrocellulose membrane, 30. liquid taking device, 31. protective shell, 32. sample pad, 211. detection strip of the first test strip, 221. detection strip of the second test strip, 311. folding line. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solutions and advantages of the embodiments of the present application more clear, the technical solutions of the embodiments of the present application will be described clearly and completely in combination with the drawings of the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all the embodiments. Based on the described embodiments of the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0027] Unless otherwise defined, the technical terms or scientific terms used herein should be understood as the usual meaning understood by those skilled in the art. The "first", "second" and similar words used in the patent application specification and claims of the present application do not represent any order, quantity or importance, but are only used to distinguish different components. Similarly, "one" or "a" and similar words do not represent a quantity limit, but represent the existence of at least one.

[0028] As Figures 1 to 3 shown, a detection device for rapid detection of His-tag protein, comprising: card box 10, top two sides inwardly inclined; detection card 20, sealed in card box 10 and top abuts card box 10 inwardly inclined, card box 10 width and detection card 20 width are adapted; liquid taking device 30, connected with detection card 20 and extending out of card box 10.

[0029] ​Specifically, the detection card 20 comprises a PVC base plate, as shown in Figure 2 、 Figure 3 The first test strip 21 and the second test strip 22 are symmetrically arranged on the PVC base plate. The first test strip 21 and the second test strip 22 both comprise a sample pad 25, a colloidal gold pad 26, a nitrocellulose membrane 27 and an absorbent paper 24 which are sequentially overlapped. The nitrocellulose membrane is provided with a detection strip and a quality control strip 23. The colloidal gold pad 26 is coated with microspheres of a first anti-HIS-tag protein antibody. The detection strip 211 of the first test strip is coated with microspheres of a second anti-HIS-tag protein antibody. The detection strip 221 of the second test strip is coated with microspheres of a third anti-HIS-tag protein antibody. The quality control strip 23 is coated with microspheres of a goat anti-mouse IgG polyclonal antibody. If the sample to be detected contains His-tag protein, the His-tag protein specifically binds to the first anti-HIS-tag protein antibody coated on the colloidal gold pad 26 to form a complex, and then continues to move forward. During the process of passing through the nitrocellulose membrane 27, the aforementioned formed complex can specifically bind to the second anti-HIS-tag protein antibody or the third anti-HIS-tag protein antibody, so that a red color appears at the detection strip, indicating that the sample to be detected is positive, i.e. containing His-tag protein.

[0030] Specifically, the detection strip is located on one side of the nitrocellulose membrane 27 close to the colloidal gold pad 26, and the quality control strip 23 is located on the other side of the nitrocellulose membrane 27 away from the colloidal gold pad 26.

[0031] Specifically, the top of the card box 10 is inwardly inclined on both sides to form a portion with a reduced inner diameter, and the detection card 20 can abut against this portion when being placed inside, so as to facilitate judging whether the detection card 20 is placed in place.

[0032] Specifically, after the first test strip 21 and the second test strip 22 are overlapped, they can be fixed on the PVC base plate by pasting.

[0033] In this embodiment, the liquid taking device 30 comprises a sampling pad 32 and a protective shell 31. The sampling pad 32 is overlapped with the sample pad 25, and the part of the sampling pad 32 extending out of the card box 10 is sealed in the protective shell 31.

[0034] Specifically, the upper part of the protective shell 31 is provided with a break line 311, and the protective shell 31 is broken along the break line 311 to expose the sampling pad 32.

[0035] Specifically, the bottom of the sampling pad 32 can be in an inverted conical shape, and the protective shell 31 can be in a cylindrical shape.

[0036] In this embodiment, the card box 10 is a box body made of transparent plastic. The whole detection process and detection result can be directly observed through the card box 10, without the need to additionally set an observation port, thereby reducing the manufacturing process and time of the card box 10, and the overall strength is better.

[0037] Specifically, the card box 10 is rectangular as a whole.

[0038] In the embodiment, the nitrocellulose membrane 27 is overlapped on the lower side of the colloidal gold pad 26 and the water absorption paper 24, so that the fixation of the nitrocellulose membrane 27 is more conducive to the detection.

[0039] Specifically, the colloidal gold particles used in the colloidal gold pad 26 have a particle size of 20-60 nanometers.

[0040] Specifically, the material of the microspheres is polystyrene.

[0041] In the embodiment, the use process and principle of the detection device for rapidly detecting His-tagged proteins are as follows: in use, the outer protective device of the sampling pad 32 is directly broken and folded, the specially-made sampling pad 32 is exposed and inserted into the sample solution, and observation can be performed. If the sample solution and the colloidal gold pad 26 marked first anti-His-tagged protein antibody on the detection reagent kit specifically bind to form a complex, and then continue to move forward, and the complex specifically binds to the second anti-His-tagged protein antibody of the first test strip 21 and the third anti-His-tagged protein antibody of the second test strip 22 during the process of passing through the nitrocellulose membrane 27, a red color appears at the detection strip, indicating that the sample to be tested is positive, that is, the sample contains His-tagged proteins. If the sample does not contain His-tagged proteins, no red color appears at the detection strip, and the sample is negative, that is, the sample does not contain His-tagged proteins. The colloidal gold-labeled first anti-His-tagged protein antibody continues to move forward to the goat anti-mouse IgG polyclonal antibody of the quality control strip 23 and binds to the goat anti-mouse IgG polyclonal antibody, forming a red strip, which is the quality control line. Whether the sample contains His-tagged proteins or not, the above-mentioned colloidal gold-labeled anti-His-tagged protein antibody 1 will continue to move forward to the quality control strip 23 and bind to the goat anti-mouse IgG polyclonal antibody coated thereon, forming a red strip, which is the quality control line. If no red color appears in the quality control line during the detection process, it proves that the colloidal gold is ineffective or the operation is wrong, and the result is invalid, which needs to be re-detected.

[0042] In summary, the technical scheme of the present application has the following beneficial effects:

[0043] 1. The utility model discloses a colloidal black gold technology and protein specific reaction, and according to the detection card sample test line and quality control line, His-tagged proteins in the detection sample can be rapidly detected. The detection device of the present application is convenient to use, rapid in detection, high in sensitivity and accurate in result.

[0044] 2. The detection device of the present application contains two test strips in parallel, and the sample can be detected twice at a time, which greatly increases the accuracy of the detection result.

[0045] 3. The card box is made of transparent plastic, the whole detection process and detection result can be directly observed through the card box, it is not necessary to additionally set an observation port, the manufacturing process and time of the card box are reduced, and meanwhile, the overall strength is better.

[0046] The above merely is exemplary implementation manners of the application, and is not used for limiting the protection scope of the application, and the protection scope of the application is determined by the appended claims.

Claims

1. A detection device for rapid detection of His-tagged proteins, characterized in that, The application relates to a card box and a detection card. The card box is internally inclined on the top of two sides. The detection card is sealed in the card box and abuts against the internally inclined part of the card box on the top. The liquid taking device is connected with the detection card and extends out of the card box. The detection card comprises a PVC bottom plate, and first and second test strips are symmetrically arranged on the PVC bottom plate. The first and second test strips each comprise a sample pad, a colloidal gold pad, a nitrocellulose membrane and a water absorption paper which are sequentially connected. 2.The detection device for rapidly detecting His-tagged protein according to claim 1, characterized in that, The colloidal gold pad is coated with microspheres of a first anti-HIS tag protein antibody. 3.The detection device for rapid detection of His-tag protein according to claim 2, characterized in that, The detection strip of the first test strip is coated with microspheres of a second anti-HIS tag protein antibody. 4.The detection device for rapidly detecting His-tagged protein according to claim 2, characterized in that, The detection strip of the second test strip is coated with microspheres of a third anti-HIS tag protein antibody. 5.The detection device for rapid detection of His-tag protein according to claim 1, characterized in that, The quality control strip is coated with microspheres of a goat anti-mouse IgG polyclonal antibody. 6.The detection device for rapid detection of His-tag protein according to claim 1, characterized in that, The liquid taking device comprises a sampling pad and a protective shell. 7.The detection device for rapid detection of His-tag protein according to claim 1, characterized in that, The sampling pad is connected with the sample pad and the part of the sampling pad extending out of the card box is sealed in the protective shell. 8.The detection device for rapidly detecting His-tagged protein according to claim 1, characterized in that, The upper part of the protective shell is provided with a folding line. 9.The detection device for rapidly detecting His-tagged protein according to claim 1, characterized in that, The protective shell is folded along the folding line to expose the sampling pad. 10.The detection device for rapidly detecting His-tagged protein according to claim 1, characterized in that, The bottom of the sampling pad is in an inverted conical shape. The protective shell is in a cylindrical shape. The card box is made of transparent plastic. The two sides of the nitrocellulose membrane are connected with the colloidal gold pad and the water absorption paper respectively. The card box is in a rectangular shape. The first and second test strips are fixed on the PVC bottom plate by pasting. The detection strip is located on the side of the nitrocellulose membrane close to the colloidal gold pad. The quality control strip is located on the side of the nitrocellulose membrane away from the colloidal gold pad. The material of the microspheres is polystyrene.

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