Multi-camera switching device

By controlling the movement of the fluorescence field component within the microscope apparatus and adjusting the alignment between the camera center and the optical path center, the accuracy problem in dual-camera imaging was solved, achieving high-quality bright-field and fluorescence imaging.

CN223728055UActive Publication Date: 2025-12-26HANGZHOU ALLSHENG INSTR
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Patent Information

Application Number
CN202520171805.6
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2025-01-24
Publication Date
2025-12-26
Estimated Expiration
2035-01-24

AI Technical Summary

Technical Problem

In existing microscope setups, the center of the camera is not aligned with the center of the optical path during dual-camera imaging, resulting in low imaging accuracy and the inability to simultaneously achieve high-quality bright-field and fluorescence imaging.

Method used

By controlling the movement of the fluorescence field component at the first and second positions, the alignment between the camera center and the optical path center is adjusted, and the alignment between the camera center and the optical path center is achieved using a reflective element and a motor drive system.

Benefits of technology

It improves the accuracy of microscopic imaging, ensures that the camera center and the optical path center are aligned in different modes, and enhances the image quality.

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Abstract

The utility model provides a multi-camera switching device. The multi-camera switching device comprises a lighting source, an objective lens and a first reflecting element, wherein the objective lens and the first reflecting element are sequentially arranged in the light beam direction of the lighting source; the light path center of the first reflecting element is aligned with the centers of the imaging tube lens and the first camera; the fluorescent field assembly can move to a first position and a second position and comprises a fluorescent module and a second reflecting element which are located on the two sides of the imaging tube lens; when the fluorescent field assembly is at the first position, the fluorescent field assembly is located on a light path between the first reflecting element and the first camera; when the fluorescent field assembly is at the second position, the fluorescent field assembly is not on a light path between the first reflecting element and the first camera; and the center of the second camera is aligned with the center of the light path of the fluorescent field assembly. By moving the fluorescent field assembly to the first position or the second position, the path of the light path is changed, so that the centers of the first camera and the second camera can be aligned with the center of the light path in different modes, and the problem that the centers of multiple cameras are inconsistent with the center of the light path is solved.
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Description

TECHNICAL FIELD

[0001] The present application relates to the field of microscope imaging technology, in particular to a multi-camera switching device. BACKGROUND

[0002] Microscope imaging technology has a wide and important application in the field of industry and life science, and most of the microscopes used in life science research need to have the functions of bright field imaging and fluorescence imaging. Bright field imaging is an important means of visualizing research; fluorescence imaging is an important tool for observing the distribution of specific molecules and performing real-time dynamic imaging of living cells or tissues.

[0003] If fluorescence imaging is realized by a black and white camera, it is impossible to realize color bright field imaging at the same time; or in order to obtain high-quality color bright field imaging, a color camera is used, which will sacrifice the fluorescence imaging function, resulting in a decrease in signal receiving efficiency and performance of fluorescence imaging.

[0004] To solve the above problems, the prior art provides a dual-camera imaging device, but the dual-camera imaging device will have a situation that the centers and focal lengths of the two cameras are inconsistent during shooting, resulting in a problem that the centers of the two cameras are inconsistent with the center of the optical path, and the imaging precision is low. SUMMARY

[0005] The purpose of the present application is to provide a multi-camera switching device, which adjusts the consistency of the camera center and the optical path center by controlling the movement of the fluorescence field assembly in the first position and the second position.

[0006] The present application provides a multi-camera switching device, comprising:

[0007] An illumination light source, an objective lens and a first reflecting element arranged in sequence along the beam direction of the illumination light source;

[0008] An imaging tube lens and a first camera, the optical path center of the first reflecting element is aligned with the center of the imaging tube lens and the first camera;

[0009] A fluorescence field assembly, the fluorescence field assembly comprises a fluorescence module and a second reflecting element; the fluorescence module and the second reflecting element are located on both sides of the imaging tube lens; the fluorescence field assembly is movable to a first position and a second position; when the fluorescence field assembly is in the first position, the fluorescence field assembly is located on the optical path between the first reflecting element and the first camera; when the fluorescence field assembly is in the second position, the fluorescence field assembly is not on the optical path between the first reflecting element and the first camera;

[0010] A second camera, the center of the second camera is aligned with the optical path center of the fluorescence field assembly.

[0011] In an embodiment, the first reflecting element and / or the second reflecting element each comprises:

[0012] A mirror plate for reflecting light rays;

[0013] A fixed plate rigidly connected with the mirror plate;

[0014] An adjusting plate movably connected with the fixed plate, at least one movable element is arranged between the adjusting plate and the fixed plate, the movement of the movable element drives the fixed plate to move, so that the mirror plate is tilted and yawed.

[0015] In an embodiment, the device further comprises:

[0016] A first linear guide rail, the first linear guide rail is perpendicular to the line connecting the first reflecting element and the first camera;

[0017] A first motor, the first motor is used to drive the fluorescent module to move on the first linear guide rail.

[0018] In an embodiment, the device further comprises:

[0019] A first positioning element, the first positioning element comprises a first fixed seat, a first positioning spring and a first bearing connected in sequence, when the fluorescent module moves to the first position, the first bearing is clamped with the base groove of the fluorescent module.

[0020] In an embodiment, the device further comprises:

[0021] A second linear guide rail, the second linear guide rail is perpendicular to the line connecting the first reflecting element and the first camera;

[0022] A second motor, the second motor is used to drive the second reflecting element to move on the second linear guide rail.

[0023] In an embodiment, the device further comprises:

[0024] A second positioning element, the second positioning element comprises a second fixed seat, a second positioning spring and a second bearing connected in sequence, when the second reflecting element moves to the first position, the second bearing is clamped with the base groove of the reflecting element.

[0025] In an embodiment, the device further comprises:

[0026] A turntable, the fluorescent module and / or the second reflecting element is fixed on the turntable, the turntable is used to drive the fluorescent module and / or the second reflecting element to switch between the first position and the second position.

[0027] In an embodiment, the second camera comprises:

[0028] A fluorescent camera;

[0029] An adjusting seat;

[0030] The third fixing seat has a fluorescent camera at one end and an adjusting seat at the other end.

[0031] The eccentric cam is used to move the adjusting seat in a direction perpendicular to the third fixing seat.

[0032] In an embodiment, the eccentric cam comprises:

[0033] The first cylinder is fixed with the adjusting seat.

[0034] The second cylinder is connected with the first cylinder through a connecting piece, and the centers of the first cylinder and the second cylinder are not on the same straight line.

[0035] In an embodiment, the second camera further comprises:

[0036] The positioning pin is connected with the connecting piece connecting the second cylinder and the first cylinder, and is used to fix the eccentric cam.

[0037] Compared with the prior art, the application has the following beneficial effects:

[0038] The application can control the movement of the fluorescent field assembly. When the fluorescent field assembly is in a first position, the fluorescent field assembly is located on a light path between the first reflecting element and the first camera, so that the center of the second camera is aligned with the center of the light path. When the fluorescent assembly is in a second position, the fluorescent assembly is not on the light path between the first reflecting element and the first camera, so that the center of the first camera is aligned with the center of the light path. In different modes, the centers of the first camera and the second camera are respectively aligned with the center of the light path, thereby improving the imaging accuracy. BRIEF DESCRIPTION OF DRAWINGS

[0039] In order to more clearly illustrate the technical solutions of the embodiments of the application, the following will briefly introduce the drawings needed to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor.

[0040] Figure 1 is a schematic diagram of a bright field mode provided by an embodiment of the application;

[0041] Figure 2 is a schematic diagram of a fluorescent mode provided by an embodiment of the application;

[0042] Figure 3 is a schematic diagram of a reflecting element provided by an embodiment of the application;

[0043] Figure 4 is a schematic diagram of a first positioning member provided by an embodiment of the application;

[0044] Figure 5 is a schematic view of a second positioning member provided by an embodiment of the present application;

[0045] Figure 6 is a schematic view of a rotating disc driving mode 1 provided by an embodiment of the present application;

[0046] Figure 7 is a schematic view of a rotating disc driving mode 2 provided by an embodiment of the present application;

[0047] Figure 8 is a schematic view of a camera structure provided by an embodiment of the present application;

[0048] Figure 9 is a schematic view of an eccentric cam structure provided by an embodiment of the present application.

[0049] Among the above drawings, the following reference signs are included:

[0050] 1 - illumination light source; 2 - objective lens; 3 - first reflecting element; 4 - tube lens;

[0051] 5 - first camera; 6 - second camera;

[0052] 7 - fluorescent field assembly; 8 - fluorescent module; 9 - second reflecting element;

[0053] 10 - reflecting mirror plate; 11 - fixed plate; 12 - adjusting plate; 13 - movable member; 14 - steel ball; 15 - adjusting screw;

[0054] 16 - first motor; 17 - first linear guide rail; 18 - first positioning member; 19 - first fixed seat; 20 - first positioning elastic sheet; 21 - first bearing;

[0055] 22 - second motor; 23 - second linear guide rail; 24 - second positioning member; 25 - second fixed seat; 26 - second positioning elastic sheet; 27 - second bearing;

[0056] 28 - rotating disc;

[0057] 29 - fluorescent camera; 30 - adjusting seat; 31 - third fixed seat; 32 - eccentric cam;

[0058] 33 - first cylinder; 34 - second cylinder; 35 - positioning pin. DETAILED DESCRIPTION

[0059] The terms "first", "second", "third", etc. are only used for differentiation and description, and do not represent the arrangement serial number, and cannot be understood as indicating or implying relative importance.

[0060] In addition, the terms "horizontal", "vertical", "overhang", and the like do not mean that the components must be absolutely horizontal or overhanging, but can be slightly inclined. For example, "horizontal" only means that it is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly inclined.

[0061] In the description of the present application, it should be noted that the terms "in", "out", "left", "right", "up", "down" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship commonly used when the product of the present application is used, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0062] The present application provides a multi-camera switching device, Figure 1 is a bright field mode schematic diagram provided by an embodiment of the present application, Figure 2 is a fluorescence mode schematic diagram provided by an embodiment of the present application, as Figures 1-2 shown, the multi-camera switching device comprises: an illumination light source 1, an objective lens 2 and a first reflecting element 3 arranged in turn along the beam direction of the illumination light source 1, an imaging tube lens 4, a first camera 5, a fluorescence field assembly 7 and a second camera 6; wherein the objective lens 2 and the tube lens 4 are used for magnifying imaging, the optical path center of the first reflecting element 3 is aligned with the center of the imaging tube lens 4 and the first camera 5; the fluorescence field assembly 7 comprises a fluorescence module 8 and a second reflecting element 9; the fluorescence module 8 and the second reflecting element 9 are located on both sides of the imaging tube lens 4; the fluorescence field assembly 7 is movable to a first position and a second position; the center of the second camera 6 is aligned with the optical path center of the fluorescence field assembly 7.

[0063] Wherein, the first position refers to that the fluorescence field assembly 7 is located on the optical path between the first reflecting element 3 and the first camera 5, and the second position refers to that the fluorescence assembly is not on the optical path between the first reflecting element 3 and the first camera 5.

[0064] Specifically, in microscopic imaging, the sample is placed between the illumination light source 1 and the objective lens 2, when the fluorescence field assembly 7 is in the first position, the fluorescence field assembly 7 is located on the optical path between the first reflecting element 3 and the first camera 5, the light beam emitted by the light source in the fluorescence module 8 is reflected to the first reflecting element 3 through the dichroic mirror in the fluorescence module 8, the first reflecting element 3 reflects the light beam to the sample, after the sample absorbs the light beam, another light beam which can penetrate the dichroic mirror is reflected, the light beam which can penetrate the dichroic mirror passes through the objective lens 2, the fluorescence module 8, the tube lens 4 and the second reflecting element 9 in turn, is reflected to the second camera 6 which is aligned with the optical path center thereof by the second reflecting element 9, and the second camera 6 images.

[0065] Specifically, in the microscopic imaging, the sample is placed between the illumination light source 1 and the objective lens 2, when the fluorescence module is in the second position, the fluorescence module is not in the light path between the first reflecting element 3 and the first camera 5; the light beam emitted by the illumination light source 1 passes through the sample to the first reflecting element 3, the light reflected by the first reflecting element 3 passes through the tube lens 4 to the first camera 5 which is aligned with the center of the light path, and the first camera 5 images.

[0066] The application uses the movement of the fluorescence module 7 between the first position and the second position to change the path of the light path, so that when the fluorescence module 7 is in the first position, the center of the second camera 6 is aligned with the center of the light path; when the fluorescence module 7 is in the second position, the center of the first camera 5 is aligned with the center of the light path, which ensures that different cameras can be aligned with the center of the light path in different working modes, thereby significantly improving the imaging quality.

[0067] In an embodiment, Figure 3 is a schematic diagram of the reflecting element provided by an embodiment of the application, as Figure 3 shown, the first reflecting element 3 and / or the second reflecting element 9 comprises a reflecting mirror plate 10, a fixed plate 11 and an adjusting plate 12; wherein the reflecting mirror plate 10 is used for reflecting light, the fixed plate 11 is rigidly connected with the reflecting mirror plate 10, and the adjusting plate 12 is movably connected with the fixed plate 11; at least one movable element 13 is arranged between the adjusting plate 12 and the fixed plate 11, the movement of the movable element 13 drives the fixed plate 11 to move, so that the reflecting mirror plate 10 is pitch and yawed; for example, the movable element 13 is a steel ball 14 and an adjusting screw 15, the adjusting screw 15 is arranged on the adjusting plate 12, the screw tip of the adjusting screw 15 is in contact with the steel ball 14, and the movement of the steel ball 14 is driven by rotating the adjusting screw 15, so that the fixed plate 11 is moved and the reflecting mirror plate 10 is pitch and yawed, so that the reflecting mirror plate 10 is aligned with the center of the light path.

[0068] In an embodiment, as Figures 1-2 shown, the multi-camera switching device further comprises a first motor 16 and a first linear guide rail 17, the first linear guide rail 17 is perpendicular to the line connecting the first reflecting element 3 and the first camera 5; the first motor 16 is used to drive the fluorescence module 8 to move on the first linear guide rail 17, wherein the movement of the fluorescence module 8 can also be realized manually.

[0069] In an embodiment, Figure 4 is a schematic diagram of the first positioning element provided by an embodiment of the application, as Figures 1-2As shown in FIG. 4, the multi-camera switching device further comprises a first positioning member 18, which comprises a first fixing seat 19, a first positioning spring 20 and a first bearing 21 connected in sequence. When the fluorescence module 8 moves to the first position, the first positioning spring 20 releases the internal force stored by its elastic deformation to press the first bearing 21 fixed thereto into the base groove of the fluorescence module 8, so that the first bearing 21 is clamped with the base groove of the fluorescence module 8.

[0070] In an embodiment, as shown in FIG. 6, the multi-camera switching device further comprises a second linear guide rail 23 and a second motor 22. The second linear guide rail 23 is perpendicular to the line between the first reflecting element 3 and the first camera 5. The second motor 22 is used to drive the second reflecting element 9 to move on the second linear guide rail 23. The movement of the second reflecting element 9 can also be realized manually. Figures 1-2

[0071] In an embodiment, as shown in FIG. 5, the multi-camera switching device further comprises a second positioning member 24, which comprises a second fixing seat 25, a second positioning spring 26 and a second bearing 27 connected in sequence. When the second reflecting element 9 moves to the first position, the second positioning spring 26 releases the internal force stored by its elastic deformation to press the first bearing 21 fixed thereto into the base groove of the second reflecting element 9, so that the second bearing 27 is clamped with the base groove of the second reflecting element 9. Figure 5 Figures 1-2 In an embodiment, as shown in FIG. 6, the multi-camera switching device further comprises a second linear guide rail 23 and a second motor 22. The second linear guide rail 23 is perpendicular to the line between the first reflecting element 3 and the first camera 5. The second motor 22 is used to drive the second reflecting element 9 to move on the second linear guide rail 23. The movement of the second reflecting element 9 can also be realized manually.

[0072] In an embodiment, as shown in FIG. 6, the multi-camera switching device further comprises a second linear guide rail 23 and a second motor 22. The second linear guide rail 23 is perpendicular to the line between the first reflecting element 3 and the first camera 5. The second motor 22 is used to drive the second reflecting element 9 to move on the second linear guide rail 23. The movement of the second reflecting element 9 can also be realized manually. Figure 6 Figure 7 In an embodiment, as shown in FIG. 6, the multi-camera switching device further comprises a second linear guide rail 23 and a second motor 22. The second linear guide rail 23 is perpendicular to the line between the first reflecting element 3 and the first camera 5. The second motor 22 is used to drive the second reflecting element 9 to move on the second linear guide rail 23. The movement of the second reflecting element 9 can also be realized manually. Figures 6-7 In an embodiment, as shown in FIG. 6, the multi-camera switching device further comprises a second linear guide rail 23 and a second motor 22. The second linear guide rail 23 is perpendicular to the line between the first reflecting element 3 and the first camera 5. The second motor 22 is used to drive the second reflecting element 9 to move on the second linear guide rail 23. The movement of the second reflecting element 9 can also be realized manually. ​​​

[0073] In an embodiment, Figure 8 is a schematic diagram of a camera structure provided by an embodiment of the present application, Figure 9 is a schematic diagram of an eccentric cam structure provided by an embodiment of the present application, as Figures 8-9 As shown, the second camera 6 comprises a fluorescence camera 29, an adjusting seat 30, a third fixing seat 31, and an eccentric cam 32; one end of the third fixing seat 31 is the fluorescence camera 29, and the other end is the adjusting seat 30; the eccentric cam 32 is used to move the adjusting seat 30 in a direction perpendicular to the third fixing seat 31;

[0074] The eccentric cam 32 comprises a first cylinder 33 and a second cylinder 34; the first cylinder 33 is fixed with the adjusting seat 30; the second cylinder 34 and the first cylinder 33 are connected through a connecting piece; the centers of the first cylinder 33 and the second cylinder 34 are not on the same straight line; the first cylinder 33 can be rotated by rotating the second cylinder 34; since the centers of the first cylinder 33 and the second cylinder 34 are not on the same straight line, the center of the second cylinder 34 will be displaced when the second cylinder 34 moves, thereby driving the adjusting seat 30 fixed with the second cylinder 34 to be displaced in a direction along a line connecting the third fixing seat 31 and the fluorescence camera 29.

[0075] In an embodiment, the second camera 6 further comprises a positioning pin 35 connected with the connecting piece connecting the second cylinder 34 and the first cylinder 33, used to fix the eccentric cam 32; when the adjusting seat 30 moves with the second cylinder 34 to find the clearest picture, the positioning pin 35 is inserted on the connecting piece to fix the eccentric cam 32 immovably, at this time, the focal plane of the second camera 6 is the same as that of the first camera 5.

[0076] The first camera 5 and the second camera 6 can be respectively moved in a direction along a straight line with the center of the optical path aligned, to realize the focal plane consistency of multiple cameras.

[0077] The present application has a simple structure; the focal plane of the second camera 6 is made the same as that of the first camera 5 by adjusting the eccentric cam 32 of the second camera 6, then the pitch angle and the yaw angle of the mirror plate 10 of the second reflecting mirror element are adjusted to align the center of the second camera 6 with the center of the optical path, after that, in the subsequent microscopic imaging process, only the fluorescence field assembly 7 needs to be moved in the first position and the second position, to realize the imaging of the first camera 5 or the second camera 6; the present application only needs to align the centers of the first camera 5 and the second camera 6 with the center of the optical path before use, and adjust the focal length, and in the subsequent use process, the alignment and the focal length adjustment are no longer needed, thereby solving the problems of difficult alignment of the camera center with the optical path center and difficult focal length adjustment when multiple cameras are switched.

[0078] In the description of the application, unless otherwise clearly specified and limited, the terms "set", "install", "connect", "connect" should be understood broadly, for example, can be fixedly connected, can also be detachably connected, or integrally connected; can be mechanically connected, can also be electrically connected; can be directly connected, can also be indirectly connected through an intermediate medium, can be the communication inside two elements.

[0079] It should be noted that the features in the embodiments of the present application can be combined with each other without conflict. The above is only the preferred embodiment of the present application and is not used to limit the present application. For those skilled in the art, the present application can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A multi-camera switching apparatus, characterized by, The device comprises: a light source, an objective lens and a first reflecting element arranged in sequence along the beam direction of the light source; an imaging tube and a first camera, the optical path center of the first reflecting element is aligned with the center of the imaging tube and the first camera; a fluorescence field assembly, the fluorescence field assembly comprises a fluorescence module and a second reflecting element; the fluorescence module and the second reflecting element are located on both sides of the imaging tube; the fluorescence field assembly is movable to a first position and a second position; when the fluorescence field assembly is in the first position, the fluorescence field assembly is located on the optical path between the first reflecting element and the first camera; when the fluorescence field assembly is in the second position, the fluorescence field assembly is not on the optical path between the first reflecting element and the first camera; a second camera, the center of the second camera is aligned with the optical path center of the fluorescence field assembly.

2. The multi-camera switching apparatus according to claim 1, characterized by The first reflecting element and / or the second reflecting element each comprises: a mirror plate for reflecting light; a fixed plate rigidly connected with the mirror plate; an adjusting plate movably connected with the fixed plate, at least one movable element is arranged between the adjusting plate and the fixed plate, the movement of the movable element drives the movement of the fixed plate, so that the mirror plate is pitch and yawed.

3. The multi-camera switching apparatus according to claim 1, characterized by Further comprising: a first linear guide rail, the first linear guide rail is perpendicular to the line connecting the first reflecting element and the first camera; a first motor for driving the fluorescence module to move on the first linear guide rail.

4. The multi-camera switching apparatus according to claim 3, characterized by The device further comprises: a first positioning element, the first positioning element comprises a first fixed seat, a first positioning spring and a first bearing connected in sequence, when the fluorescence module moves to the first position, the first bearing is clamped with the base groove of the fluorescence module.

5. The multi-camera switching apparatus according to claim 1, characterized by Further comprising: a second linear guide rail, the second linear guide rail is perpendicular to the line connecting the first reflecting element and the first camera; a second motor for driving the second reflecting element to move on the second linear guide rail.

6. The multi-camera switching apparatus according to claim 5, wherein The device further comprises: a second positioning element, the second positioning element comprises a second fixed seat, a second positioning spring and a second bearing connected in sequence, when the second reflecting element moves to the first position, the second bearing is clamped with the base groove of the reflecting element.

7. The multi-camera switching apparatus according to claim 1, characterized by Further comprising: a turntable, the fluorescence module and / or the second reflecting element are fixed on the turntable, the turntable is used to drive the fluorescence module and / or the second reflecting element to switch between the first position and the second position.

8. The multi-camera switching apparatus according to claim 1, characterized by The second camera comprises: a fluorescence camera; an adjusting seat; a third fixed seat, one end of the third fixed seat is the fluorescence camera, and the other end is the adjusting seat; an eccentric cam for moving the adjusting seat along a direction perpendicular to the third fixed seat.

9. The multi-camera switching apparatus according to claim 8, characterized by The eccentric cam comprises: a first cylinder fixed with the adjusting seat; a second cylinder connected with the first cylinder through a connecting element, the centers of the first cylinder and the second cylinder are not on the same straight line.

10. The multi-camera switching apparatus according to claim 9, wherein, The second camera further comprises: A positioning pin connected with a connecting piece connecting the second cylinder and the first cylinder, for fixing the eccentric cam.