Piston with pipetting function and multifunctional cavity structure

Abstract

The utility model provides a piston with a pipetting function and a multifunctional cavity structure, and belongs to the technical field of medical detection, the piston comprises a piston block, the piston block is provided with a first valve hole, two ports of the first valve hole are respectively positioned on two end surfaces of the piston block, and spaces on two sides of the piston block are allowed to be communicated through the first valve hole; a first one-way valve is mounted in the first valve hole; when the piston block is static or moves towards one movement direction, the first one-way valve is in a closed state, and when the piston block moves towards the other movement direction, the first one-way valve is allowed to be opened. The utility model has the beneficial effects that when the piston block is static or moves towards other directions, the two cavities can be isolated, and when the piston block moves towards a set direction, the first one-way valve is opened, so that liquid is transferred from one side of the piston block to the other side of the piston block, and a liquid guide pipe or a connector does not need to be designed outside the cavities; after the piston is applied, the compactness of the device can be greatly improved, and the equipment outline is reduced.

Description

TECHNICAL FIELD

[0001] The utility model belongs to medical detection technical field relates to a piston with pipetting function and multi -functional chamber structure. BACKGROUND

[0002] The technology for detecting circulating tumor cells (CTCs) based on surface-enhanced Raman scattering (SERS) is a detection method for identifying and quantifying CTCs from peripheral blood. The specific steps of the detection method are as follows: 1, preparing to collect patient blood samples; 2, mixing and culturing the blood sample with SERS active nanomaterials; 3, using an external magnetic field to enrich CTCs-magnetic bead complexes from the blood; 4, separating the waste liquid from the enriched CTCs; 5, using buffer solution for multiple washing to collect CTCs-magnetic bead complexes; 6, placing the collected CTCs-magnetic bead complexes in a Raman spectrometer for detection.

[0003] At present, there is an invention patent with the application number CN202010514536.0, named circulating tumor cell screening and separation device and method and application. The device has the functions of realizing steps 2 to 5 described above. It should be noted that the device realizes the flow of liquid in the blood sample incubation chamber by the movement of the piston in cooperation with the liquid guide pipe, so that the device can realize reaction incubation, enrichment, separation, cleaning and other functions in one blood sample incubation chamber, greatly improving the integration of the device and achieving the purpose of miniaturization and portability.

[0004] However, the above-mentioned device must rely on the external liquid guide pipe to control the flow of liquid in the blood sample incubation chamber by the movement of the piston, which means that two joints communicating with the blood sample incubation chamber must be provided outside the device shell, so that the device has further optimization space in terms of compactness and volume. And the presence of the liquid guide pipe increases the difficulty of the cleaning step, and the CTCs-magnetic bead complexes may be difficult to completely clean in the liquid guide pipe, resulting in deviation of the detection result, so there is further optimization space. UTILITY MODEL CONTENTS

[0005] The utility model aims at the above-mentioned problems existing in the prior art, and proposes a piston with pipetting function and multi -functional chamber structure.

[0006] The purpose of the utility model can be realized by the following technical scheme: a piston with pipetting function, comprising:

[0007] The piston block is provided with a first valve hole, two ports of the first valve hole are respectively located at two end faces of the piston block, spaces on two sides of the piston block are allowed to be communicated through the first valve hole, and a first one-way valve is arranged in the first valve hole; the first one-way valve is in a closed state when the piston block is static or moves in one movement direction, and the first one-way valve is allowed to be opened when the piston block moves in another movement direction.

[0008] Preferably, the piston block is provided with a plurality of first valve holes, and the first one-way valve is arranged in each first valve hole, and the first valve holes are uniformly and spacedly distributed.

[0009] Preferably, the piston block is provided with a plurality of first valve holes, and the first one-way valve is arranged in each first valve hole, and the first valve holes are uniformly and spacedly distributed.

[0010] Preferably, a movement direction of the piston block is a retracting direction of the piston rod, and another movement direction of the piston block is an extending direction of the piston rod.

[0011] Preferably, a sealing ring is sleeved on an outer peripheral surface of the piston block.

[0012] A multifunctional chamber structure comprises the piston with the pipetting function, and further comprises a shell, a mixing cavity is arranged in the shell, the piston block is movably arranged in the mixing cavity and separates the mixing cavity into an upper cavity and a lower cavity, the upper cavity and the lower cavity are isolated by the piston block, and the upper cavity is kept in a sealed arrangement; when the piston block moves in another movement direction, the volume of the upper cavity is reduced and the volume of the lower cavity is increased, and liquid in the upper cavity is allowed to push open a first one-way valve to flow to the lower cavity.

[0013] Preferably, the shell is provided with an upper cover located at the top of the mixing cavity, a piston rod penetrates through the upper cover and extends into the mixing cavity to be connected with the piston block, the upper cover is provided with a second valve hole, the upper cavity and the outside are allowed to be communicated through the second valve hole, and a second one-way valve is arranged in the second valve hole; when the piston block moves in one movement direction, the volume of the upper cavity is increased and the volume of the lower cavity is reduced, and the second one-way valve is allowed to be opened to allow outside air to enter the upper cavity.

[0014] Preferably, an outer peripheral surface of the piston block is attached to an inner wall surface of the mixing cavity, and the outer peripheral surface and the inner wall surface are sealed by a sealing ring.

[0015] Preferably, the upper cavity is arranged as a sealed cavity structure between the top of the mixing cavity and an upper end face of the piston block, and the lower cavity is arranged as a cavity structure between the bottom of the mixing cavity and a lower end face of the piston block.

[0016] Compared with the prior art, the piston block is provided with a first valve hole, two ports of the first valve hole are respectively located at two end faces of the piston block, spaces on two sides of the piston block are allowed to be communicated through the first valve hole, and a first one-way valve is arranged in the first valve hole; the first one-way valve is in a closed state when the piston block is static or moves in one movement direction, and the first one-way valve is allowed to be opened when the piston block moves in another movement direction.

[0017] 1. The piston has the function of controlling the displacement, when the piston block is at rest or moves in other directions, the two cavities can be isolated, when the piston block moves in the set direction, the first one-way valve opens to transfer the liquid from one side of the piston block to the other side of the piston block, without designing the liquid guide pipe or interface outside the cavity, after using the piston, the compactness of the device can be greatly improved and the equipment outline is reduced.

[0018] 2. The first one-way valve of the piston block is designed to make the liquid flow path more direct, reduce the liquid retention in the pipeline, reduce the risk of CTCs-magnetic bead compound adhering to the wall of the liquid guide pipe, thereby ensuring the thoroughness of the cleaning and improving the accuracy of the detection result.

[0019] 3. The piston is suitable for highly integrated small-sized screening and separation devices, which need to realize reaction cultivation, enrichment, separation and cleaning in one chamber. When the device needs to discharge waste liquid from the chamber after completing the enrichment step, the piston block can be pulled to discharge the waste liquid; similarly, when the device is in the cleaning step, the piston block can be repeatedly pulled while injecting the cleaning liquid to wash the target cells in the chamber into the screening chamber for collection. BRIEF DESCRIPTION OF DRAWINGS

[0020] Fig. 1 It is a structure schematic view of the piston of the utility model.

[0021] Fig. 2 It is an axial side view of the piston of the utility model.

[0022] Fig. 3 It is a schematic view of the multifunctional chamber structure of the utility model.

[0023] In the figure, 100, piston block; 110, first valve hole; 120, first one-way valve; 130, sealing ring; 200, piston rod; 300, shell; 310, mixing cavity; 311, upper cavity; 312, lower cavity; 320, upper cover; 321, second valve hole; 322, second one-way valve. DETAILED DESCRIPTION

[0024] The following is a specific embodiment of the utility model and further describes the technical scheme of the utility model in combination with the drawings, but the utility model is not limited to these embodiments.

[0025] As Figs. 1-3As shown, a piston with pipetting function comprises: a piston block 100, the piston block 100 is provided with a first valve hole 110, two ports of the first valve hole 110 are respectively located at two end faces of the piston block 100, spaces on both sides of the piston block 100 are allowed to be communicated through the first valve hole 110, and a first one-way valve 120 is installed in the first valve hole 110; when the piston block 100 is static or moves in one movement direction, the first one-way valve 120 is in a closed state, and when the piston block 100 moves in another movement direction, the first one-way valve 120 is allowed to be opened.

[0026] The piston block 100 refers to a structure capable of reciprocating, that is, the piston block 100 has two movement directions, and the two movement directions are opposite. The piston block 100 can isolate two cavities located on both sides thereof, the piston block 100 is internally provided with the first valve hole 110, two openings of the first valve hole 110 are respectively located on two end faces of the piston block 100, and the first valve hole 110 is designed to allow the two cavities to be communicated. A first one-way valve 120 is installed in the first valve hole 110, and the first one-way valve 120 is only allowed to flow in one direction and prevent flow in the opposite direction.

[0027] It should be pointed out here that when the piston is located in a chamber, it naturally separates the chamber into two cavities, and if no external liquid guide pipe is connected, the two cavities are not communicated, so the piston cannot move. Based on the above reasons, the current circulating tumor cell screening and separation device needs to be provided with a liquid guide pipe outside the chamber, and when the piston moves in a certain stroke range in a set direction, it can discharge the liquid in one cavity to the other cavity through the liquid guide pipe.

[0028] In the design, no external liquid guide pipe is needed outside the chamber, when the piston block 100 is static, the two cavities are isolated because the first one-way valve 120 is closed, that is, the liquid in one cavity cannot flow into the other cavity; when the piston block 100 moves in another movement direction, the liquid in one cavity can push open the first one-way valve 120 to flow to the other cavity, thereby realizing the pipetting function through the movement of the piston block 100, and the function does not need to rely on the external liquid guide pipe. When the piston block 100 moves in one movement direction, the piston block 100 can discharge the liquid in the other cavity.

[0029] The piston block 100 of this design incorporates a first one-way valve 120. The movement of the piston block 100 directly controls the liquid flow, eliminating the need for an external liquid guide tube. This simplifies the device's structure, reduces external connectors, and improves its compactness and portability. The elimination of the liquid guide tube also eliminates the need for additional pipe connection and disconnection steps during operation, simplifying the process and enhancing ease of use. More importantly, the first one-way valve 120 design of the piston block 100 makes the liquid flow path more direct, reducing liquid stagnation in the pipes and lowering the risk of CTCs-magnetic bead composites adhering to the liquid guide tube wall. This ensures thorough cleaning and improves the accuracy of the test results.

[0030] It is important to note that this piston is suitable for highly integrated, miniaturized screening and separation devices that integrate multiple functions such as reaction cultivation, enrichment, separation, and washing within a single chamber. When waste liquid needs to be discharged from the chamber after the enrichment step, the piston block 100 can be repeatedly pulled to expel it. Similarly, during the washing step, the piston block 100 can be repeatedly pulled while the washing solution is being injected to wash the target cells from the chamber into the screening chamber for collection. Furthermore, this design eliminates the need for external liquid guide tubes, making the entire device more compact and portable. Liquid transfer can be achieved without removing the liquid, thanks to the design of the first valve port 110 and the first one-way valve 120.

[0031] Based on the above embodiments, the piston block 100 is provided with a plurality of first valve holes 110, and each first valve hole 110 is provided with a first one-way valve 120, and the first valve holes 110 are evenly spaced.

[0032] The design of multiple first valve holes 110 increases the flow path of the liquid, enabling the liquid to be transferred quickly in a short time, thus improving the overall operational efficiency. The evenly distributed first valve holes 110 and first check valves 120 ensure uniform flow of liquid between the cavities on both sides of the piston block 100, reducing local pressure fluctuations caused by a single first valve hole 110 and improving the stability of the system.

[0033] like Figs. 1-2 As shown, based on the above embodiment, a piston rod 200 is also included, which is connected to the piston block 100. The piston rod 200 and piston block 100 are firmly connected, ensuring that the piston block 100 can perform stable reciprocating motion under external force. The piston rod 200 can be connected to an external drive mechanism (such as an electric motor, manual handle, etc.) or can be driven manually. The piston rod 200 allows for precise control of the movement direction and speed of the piston block 100.

[0034] On the basis of the above-mentioned embodiments, one movement direction of the piston block 100 is the retraction direction of the piston rod 200, and the other movement direction of the piston block 100 is the extension direction of the piston rod 200. In actual operation, the piston rod 200 needs to be repeatedly pulled to make the piston block 100 reciprocate to realize the pipetting function.

[0035] As shown in Fig. 1 , Fig. 3 On the basis of the above-mentioned embodiments, a sealing ring 130 is sleeved on the outer circumferential surface of the piston block 100. The main function of the sealing ring 130 is to prevent liquid from leaking from the gap between the piston block 100 and the chamber wall, and to ensure that the two cavities (the upper cavity 311 and the lower cavity 312) on both sides of the piston block 100 are completely isolated.

[0036] As shown in Figs. 1-3 On the basis of the above-mentioned embodiments, a multifunctional chamber structure includes a piston with a pipetting function, and further includes a shell 300, the shell 300 is provided with a mixing chamber 310, the piston block 100 is movably arranged in the mixing chamber 310 and divides the mixing chamber 310 into an upper cavity 311 and a lower cavity 312, the upper cavity 311 and the lower cavity 312 are isolated by the piston block 100, and the upper cavity 311 is kept in a sealed state; when the piston block 100 moves in the other movement direction, the volume of the upper cavity 311 decreases and the volume of the lower cavity 312 increases, and the liquid in the upper cavity 311 is allowed to push open the first one-way valve 120 to flow to the lower cavity 312.

[0037] The multifunctional chamber structure refers to a structure that can complete multiple functions in one chamber. In actual application, the multifunctional chamber structure is suitable for a circulating tumor cell screening and separation device. The multifunctional chamber structure can realize functions such as mixing, separation, and cleaning, which can greatly improve the integration and compactness of the device, reduce the size and external profile of the equipment, and realize miniaturization and portability of the equipment.

[0038] The shell 300 is provided with a mixing chamber 310, which is the main place for liquid processing and can perform operations such as mixing, separation, and cleaning. The mixing chamber 310 is divided by the piston block 100 into an upper cavity 311 and a lower cavity 312. The upper cavity 311 is in a closed state (i.e., the upper cavity 311 is in a sealed state under normal conditions), and the lower cavity 312 is in communication with the screening chamber.

[0039] The movement of the piston block 100 in one direction refers to the downward movement of the piston block 100, and the movement of the piston block 100 in the other direction refers to the upward movement of the piston block 100. When the piston block 100 moves upward, all the first one-way valves 120 are opened by the liquid in the upper cavity 311, and the liquid in the upper cavity 311 can flow evenly to the lower cavity 312 through the plurality of first valve holes 110; when the piston block 100 moves downward, the piston block 100 exerts pressure on the liquid in the upper cavity 311, so that the liquid in the lower cavity 312 enters the screening cavity. That is, the present scheme achieves the purpose of transferring the liquid from the upper cavity 311 to the lower cavity 312 and then to the screening cavity through the back-and-forth movement (up-and-down movement) of the piston block 100, and the design of the first one-way valve 120 enables the chamber structure to be free of external liquid guide pipes, which makes the structure of the device simpler, eliminates the need for external guide pipes, and enables the piston block 100 to move in the mixing cavity 310.

[0040] On the basis of the above-mentioned embodiments, the shell 300 has an upper cover 320 located at the top of the mixing cavity 310, the piston rod 200 passes through the upper cover 320 and extends into the mixing cavity 310 to be connected with the piston block 100, the upper cover 320 is provided with a second valve hole 321, the upper cavity 311 and the outside are allowed to communicate through the second valve hole 321, and a second one-way valve 322 is installed in the second valve hole 321; when the piston block 100 moves in one direction, the volume of the upper cavity 311 increases and the volume of the lower cavity 312 decreases, and the second one-way valve 322 is allowed to open to allow outside air to enter the upper cavity 311.

[0041] The upper cover 320 plays a sealing and protection role, prevents external pollutants from entering the mixing cavity 310, and provides an interface for installing the piston rod 200, and the upper cover 320 and the piston rod 200 are sealed by a sealing ring, so that when the piston rod 200 extends out (i.e., the piston block 100 moves upward), the liquid in the upper cavity 311 will not leak out from the gap between the upper cover 320 and the piston rod 200.

[0042] The piston rod 200 can also push the piston block 100 to move in a certain direction (i.e., the piston rod 200 retracts, causing the piston block 100 to move downward). At this time, all the first check valves 120 are closed, the upper chamber 311 and the lower chamber 312 remain isolated, and the piston block 100 forces the liquid in the lower chamber 312 to enter the screening chamber. During this process, the volume of the upper cavity 311 increases and the volume of the lower cavity 312 decreases. The upper cavity 311 is essentially vacuumed, making it difficult for the piston rod 200 and piston block 100 to move downwards. Therefore, a second valve hole 321 needs to be provided in the upper cover 320, and a second one-way valve 322 needs to be provided in the second valve hole 321. When the air pressure in the upper cavity 311 is less than the outside air pressure (i.e., when the volume of the upper cavity 311 increases), the second one-way valve 322 opens, and outside air can enter the upper cavity 311, so that the piston rod 200 and piston block 100 can move downwards to push the liquid in the lower cavity 312 into the screening chamber.

[0043] Based on the above embodiment, the outer peripheral surface of the piston block 100 is in contact with the inner wall surface of the mixing chamber 310, and the two are sealed by a sealing ring 130. This design ensures that the piston block 100 is in close contact with the inner wall surface of the mixing chamber 310, preventing liquid leakage.

[0044] Based on the above embodiments, the upper cavity 311 is configured as a sealed cavity structure between the top of the mixing cavity 310 and the upper end face of the piston block 100, and the lower cavity 312 is configured as a cavity structure between the bottom of the mixing cavity 310 and the lower end face of the piston block 100.

[0045] like Fig. 3 As shown, in the initial state, the piston block 100 is at the bottom of the mixing chamber 310, the upper chamber 311 has the largest volume, and the lower chamber 312 has the smallest volume. At this point, blood samples and SERS-active nanomaterials can be mixed and cultured in the upper chamber 311. After culture, enrichment is performed, and then the piston block 100 is pulled upwards, opening the first one-way valve 120 to allow waste liquid to enter the lower chamber 312 through the first valve orifice 110. During the cleaning step, cleaning fluid is injected into the upper chamber 311, and then the piston block 100 is pulled back and forth. When the piston block 100 moves upwards, it discharges the cleaning fluid into the lower chamber 312; when the piston block 100 moves downwards, it forces the cleaning fluid through the screening chamber, thus providing downward pressure during filtration.

[0046] It should be noted that all directional indicators (such as up, down, left, right, front, back, etc.) in this utility model embodiment are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicator will also change accordingly.

[0047] In addition, the descriptions such as "first", "second", "one" and the like in the present application are only for the purpose of description and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first" and "second" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "multiple" is at least two, for example, two, three, etc., unless otherwise specifically limited.

[0048] In the present application, unless otherwise specifically defined and limited, the terms "connection", "fixing" and the like should be broadly understood, for example, "fixing" can be fixed connection, or detachable connection, or integrated; can be mechanical connection, or electrical connection; can be directly connected, or indirectly connected through intermediate medium, can be the internal communication of two elements or the interaction relationship of two elements, unless otherwise specifically limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0049] In addition, the technical solutions of each embodiment of the present application can be combined with each other, but it must be based on the realization of the ordinary skilled in the art, when the combination of technical solutions appears contradictory or cannot be realized, it should be considered that the combination of technical solutions does not exist, nor is it within the protection scope required by the present application.

Claims

1. A piston having a pipetting function, characterized in that The piston block (100) is provided with a first valve hole (110), the two ports of the first valve hole (110) are located at the two end faces of the piston block (100), the spaces on both sides of the piston block (100) are allowed to communicate through the first valve hole (110), and a first one-way valve (120) is arranged in the first valve hole (110); the first one-way valve (120) is in a closed state when the piston block (100) is static or moves in one movement direction, and the first one-way valve (120) is allowed to open when the piston block (100) moves in another movement direction. The piston block (100) is provided with a plurality of first valve holes (110), and the first one-way valve (120) is arranged in each first valve hole (110), and the first valve holes (110) are uniformly and spacedly distributed.

2. A piston with pipetting function according to claim 1, characterized in that: The piston block (100) is provided with a plurality of first valve holes (110), and the first one-way valve (120) is arranged in each first valve hole (110), and the first valve holes (110) are uniformly and spacedly distributed.

3. The piston with pipetting function according to claim 1, characterized in that: The piston block (100) is provided with a plurality of first valve holes (110), and the first one-way valve (120) is arranged in each first valve hole (110), and the first valve holes (110) are uniformly and spacedly distributed.

4. A piston with pipetting function according to claim 3, characterized in that: The piston block (100) is provided with a plurality of first valve holes (110), and the first one-way valve (120) is arranged in each first valve hole (110), and the first valve holes (110) are uniformly and spacedly distributed.

5. The piston with pipetting function according to claim 1, characterized in that: The piston block (100) is provided with a plurality of first valve holes (110), and the first one-way valve (120) is arranged in each first valve hole (110), and the first valve holes (110) are uniformly and spacedly distributed.

6. A multi-functional chamber structure characterized by, The piston block (100) is provided with a plurality of first valve holes (110), and the first one-way valve (120) is arranged in each first valve hole (110), and the first valve holes (110) are uniformly and spacedly distributed.

7. A multi-functional chamber structure as claimed in claim 6, characterized in that: The piston block (100) is provided with a plurality of first valve holes (110), and the first one-way valve (120) is arranged in each first valve hole (110), and the first valve holes (110) are uniformly and spacedly distributed.

8. A multi-functional chamber structure as claimed in claim 6, wherein: The piston block (100) is provided with a plurality of first valve holes (110), and the first one-way valve (120) is arranged in each first valve hole (110), and the first valve holes (110) are uniformly and spacedly distributed. The piston block (100) is provided with a plurality of first valve holes (110), and the first one-way valve (120) is arranged in each first valve hole (110), and the first valve holes (110) are uniformly and spacedly distributed. The piston block (100) is provided with a plurality of first valve holes (110), and the first one-way valve (120) is arranged in each first valve hole (110), and the first valve holes (110) are uniformly and spacedly distributed. The piston block (100) is provided with a plurality of first valve holes (110), and the first one-way valve (120) is arranged in each first valve hole (110), and the first valve holes (110) are uniformly and spacedly distributed. 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