Automatic acid-fast staining device for mycobacterium detection slide

Abstract

The utility model discloses an automatic acid-fast staining device for a mycobacterium detection slide. Comprising a cylindrical shell, a station conversion unit arranged in the center of the cylindrical shell, and a first liquid dropping unit, a first cleaning unit, a second liquid dropping unit, a second cleaning unit, a third liquid dropping unit, a third cleaning unit and a slide blow-drying unit which are sequentially and continuously arranged around the station conversion unit, based on a standard operation process, flexible and continuous switching among multiple stations can be realized by arranging a dripping station, a cleaning station and a blow-drying station in an orderly and continuous surrounding manner, arranging a station switching unit in an annular center and controlling a switching motor to rotate, so that integrated integration and automatic treatment of primary dyeing, decoloring and re-dyeing processes are realized, manual operation is replaced, and the working efficiency is improved. The efficiency is higher; in addition, the liquid adding amount is controlled through the peristaltic pump, so that the technical problems that the dye liquor on the glass slide overflows or dries up easily and the quality of the dye liquor is affected due to the fact that the liquid adding amount is not accurate in the prior art are solved.

Description

TECHNICAL FIELD

[0001] The embodiment of the utility model belongs to the technical field of slide acid-fast staining, more particularly relates to a kind of automatic acid-fast staining device for mycobacterium detection slide. BACKGROUND

[0002] At present, acid-fast staining hot dyeing method is a kind of special use of the unique acid-fast component in the cell wall of Mycobacterium (including Mycobacterium tuberculosis) to carry out identification test technology. This method makes Mycobacterium appear red under microscope through specific staining procedure, while other types of bacteria, fungi and other microorganisms are dyed blue. The smear staining operation procedure of the acid-fast staining hot dyeing method is: 1) prepare defatted, clean slide, mark the detection date and sample number at 1 / 3 of left end; 2) carefully open the sample container, use broken bamboo stick stub end, pick the cheese-like, pus-like part in sputum specimen; 3) evenly smear about 10mm×20mm size oval sputum film at 2 / 3 of right side of slide; 4) after smear natural drying, flame fixation (flame passes under slide 4 times within 5 seconds); 5) drop carbonic acid complex red dye, cover slide; 6) flame heating until steam appears, separate from flame, keep for 5 minutes. During staining, sputum film should be covered by dyeing liquid all the time, if necessary, dyeing liquid can be added, do not make dyeing liquid boil when heating; 7) gently wash from one end of slide with clean running water; 8) tilt slide with forceps, drain water on slide; 9) add decolorizing agent, decolorize for 1 minute; 10) gently wash from one end of slide with clean running water, if decolorization is not complete, repeat step 9; 11) tilt slide with forceps, drain water on slide; 12) add methylene blue reagent, dye for 30 seconds; 13) tilt slide with forceps, drain reagent on slide; 14) gently wash from one end of slide with clean running water, wash reagent, drain water on slide; 15) place dyed picture on slide rack, natural drying; 16) microscopic bacteriological examination.

[0003] From the above operation procedure, it can be found that there are the following problems in pure use of ordinary staining rack: (1) primary dyeing, decolorization, re-dyeing process is complicated, manual operation consumes manpower; (2) inaccurate liquid addition amount can also easily cause dyeing liquid on slide to overflow or dry, affect dyeing liquid quality; (3) using flame heating is difficult to accurately control temperature, and using open flame has safety hazard, may cause fire or activate smoke alarm device. UTILITY MODEL CONTENTS

[0004] In order to solve the above defects or improvement needs of the prior art, the utility model provides a kind of automatic acid-fast staining device for mycobacterium detection slide.Based on standard operating procedure, by drop, cleaning, blow dry process, make orderly continuous ring type station layout, and work station conversion unit is arranged in annular center, by controlling conversion motor rotation, it can realize flexible continuous switching between multiple stations, realize the integration and automation of initial dyeing, decoloring, re-dyeing process, replace manual operation, more efficient;In addition, by peristaltic pump control liquid addition amount, thereby solve the technical problem that liquid addition amount is not accurate in prior art and can also easily cause the dye on the slide to overflow or dry, affect dye quality.

[0005] In order to achieve the above purpose, an automatic acid-fast staining device for mycobacterium detection slide, comprising:

[0006] Cylindrical shell, work station conversion unit arranged in the center of the cylindrical shell, first drop unit, first cleaning unit, second drop unit, second cleaning unit, third drop unit, third cleaning unit and slide blow-drying unit arranged in sequence around the work station conversion unit;Slide taking and placing area between the first drop unit and the slide blow-drying unit;The first drop unit, the second drop unit and the third drop unit are the same structure, and the first cleaning unit, the second cleaning unit and the third cleaning unit are the same structure;

[0007] By clamping the slide to the work station conversion unit, the slide is sequentially brought into the above-mentioned work station in order, the first drop unit drops phenol carmine solution to the slide, then the first cleaning unit washes with water, the second drop unit drops hydrochloric acid alcohol to the slide, then the second cleaning unit washes with water, the third drop unit drops methylene blue to the slide, then the third cleaning unit washes with water, and finally the slide blow-drying unit blows dry.

[0008] Preferably, the work station conversion unit comprises:

[0009] The conversion motor is arranged below the center of the cylindrical shell, the intermediate turntable is fixedly connected with the output shaft of the conversion motor through the vertical rotating shaft, the slide chuck is arranged on the side of the intermediate turntable and clamps the slide, the support slide groove is formed by cutting through the annular inside wall of the cylindrical shell, and the fourth slide passing groove is opened on the side of the slide taking and placing area of the cylindrical shell.

[0010] By controlling the directional rotation of the conversion motor, the other side of the slide clamped by the slide chuck is clamped into the support slide groove for support, and passes through the fourth slide passing groove.

[0011] Preferably, the first drop unit comprises:

[0012] The dye solution storage cavity is arranged on the top, the dye solution filling port is arranged above the dye solution storage cavity, the micro-injection peristaltic pump is connected with the lower surface of the dye solution storage cavity through the conduit, the dropper is connected with the output end of the micro-injection peristaltic pump, the first slide passing groove is arranged, and the visual camera is arranged upwards below the height of the slide for capturing the dyeing state.

[0013] Preferably, the bottom surface of the dye solution storage cavity is inclined along the side of the lower conduit port.

[0014] Preferably, the first cleaning unit comprises:

[0015] The clear water storage cavity is arranged on the top layer of the cylindrical shell, the clear water filling port is arranged on the top surface of the clear water storage cavity, the water pump is connected to the bottom of the clear water storage cavity through the conduit, the spray head is connected with the output port of the water pump, the seepage support plate is arranged below the height of the slide, and the second slide passing groove is arranged on the side of the first cleaning unit.

[0016] The drainage groove is arranged below the seepage support plate.

[0017] Preferably, the first cleaning unit comprises:

[0018] The second drainage rim is arranged on the upper end of the inner side of the second slide passing groove in the downward direction, and the first drainage rim is arranged on the lower end of the outer side of the second slide passing groove in the upward direction.

[0019] Preferably, the slide drying unit comprises:

[0020] The first heating sheet is arranged on the top layer of the cylindrical shell, the air blower is arranged below the first heating sheet, the porous support plate is arranged below the air blower and is higher than the height of the slide, the second heating sheet is arranged below the height of the slide, and the third slide passing groove is arranged on the side of the slide drying unit.

[0021] Overall, compared with the prior art, the above technical scheme conceived by the utility model can achieve the following beneficial effects:

[0022] (1) The automatic acid-fast staining device for mycobacterium detection slide of the utility model is based on a standard operation process, and through the drop, cleaning and drying procedures, an orderly continuous circular work station layout is formed, and the work station conversion unit 7 is arranged in the center of the ring. By controlling the rotation of the conversion motor, flexible and continuous switching between multiple work stations can be realized, the integration and automatic processing of the primary dyeing, decoloring and re-dyeing processes are realized, manual operation is replaced, and the efficiency is higher. In addition, the liquid addition amount is controlled by the peristaltic pump, thereby solving the technical problems in the prior art that the liquid addition amount is not accurate and the dye solution on the slide may be easily overflowed or dried, which affects the quality of the dye solution.

[0023] (2) The utility model discloses a kind of automatic acid-fast staining devices for mycobacterium detection slide, replace flame heating mode with heating sheet, not only realize temperature controllable, and solve the technical problem that flame heating method can induce fire or trigger smoke sensor. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 It is the overall structure schematic diagram of a kind of automatic acid-fast staining devices for mycobacterium detection slide of the utility model embodiment;

[0025] Figure 2 It is the structure schematic diagram of drop unit of a kind of automatic acid-fast staining devices for mycobacterium detection slide of the utility model embodiment;

[0026] Figure 3 It is the structure schematic diagram of cleaning unit of a kind of automatic acid-fast staining devices for mycobacterium detection slide of the utility model embodiment;

[0027] Figure 4 It is the structure schematic diagram of slide drying unit of a kind of automatic acid-fast staining devices for mycobacterium detection slide of the utility model embodiment;

[0028] In all drawings, same reference signs represent same technical features, specifically: 1-cylindrical shell, 2-first drop unit, 201-dye liquid filling port, 202-dye liquid storage cavity, 203-micro-annular peristaltic pump, 204-dropper, 205-first slide slot, 206-visual camera, 3-first cleaning unit, 301-clean water filling port, 302-clean water storage cavity, 303-water pump, 304-sprayer, 305-seepage support plate, 306-second slide slot, 307-first drainage rim, 308-second drainage rim, 4-second drop unit, 5-second cleaning unit, 6-slide drying unit, 601-first heating sheet, 602-blower, 603-perforated support plate, 604-second heating sheet, 605-third slide slot, 7-station conversion unit, 701-conversion motor, 702-vertical rotation shaft, 703-intermediate rotary table, 704-slide chuck, 705-supporting piece sliding groove, 706-fourth slide slot, 8-third drop unit, 9-third cleaning unit. DETAILED DESCRIPTION

[0029] In the description of this application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0030] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0031] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0032] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only for explaining the present utility model and are not intended to limit the present utility model. Furthermore, the technical features involved in the various embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0033] like Figures 1 to 4 As shown in this embodiment of the invention, the automated acid-fast staining device for mycobacterial detection slides includes:

[0034] The cylindrical shell 1, the work position conversion unit 7 arranged in the center of the cylindrical shell 1, the first droplet unit 2, the first cleaning unit 3, the second droplet unit 4, the second cleaning unit 5, the third droplet unit 8, the third cleaning unit 9 and the slide drying unit 6 arranged in sequence around the work position conversion unit 7, the slide taking and placing area between the first droplet unit 2 and the slide drying unit 6, the first droplet unit 2, the second droplet unit 4 and the third droplet unit 8 are of the same structure, and the first cleaning unit 3, the second cleaning unit 5 and the third cleaning unit 9 are of the same structure.

[0035] By clamping the slide to the work position conversion unit 7, the slide is sequentially taken into the above-mentioned work position, the first droplet unit 2 drops phenolsaline solution on the slide, then the first cleaning unit 3 performs water flushing, the second droplet unit 4 drops hydrochloric acid alcohol on the slide, then the second cleaning unit 5 performs water flushing, the third droplet unit 8 drops methylene blue on the slide, then the third cleaning unit 9 performs water flushing, and finally the slide drying unit 6 performs drying.

[0036] As shown in the utility model embodiment, Figure 1 The work position conversion unit 7 comprises:

[0037] The conversion motor 701 arranged below the center of the cylindrical shell 1, the intermediate turntable 703 fixedly connected with the output shaft of the conversion motor 701 through the vertical rotating shaft 702, the slide chuck 704 arranged on the side of the intermediate turntable 703 and used for clamping the slide, the supporting piece sliding groove 705 formed by cutting through the inner side wall of the cylindrical shell 1 in a ring shape, and the fourth slide passing groove 706 opened on the side of the slide taking and placing area of the cylindrical shell 1 and used for facilitating the slide to pass through.

[0038] By controlling the directional rotation of the conversion motor 701, the other side of the slide clamped by the slide chuck 704 is clamped into the supporting piece sliding groove 705 for support and passes through the fourth slide passing groove 706.

[0039] As shown in the utility model embodiment, Figure 2 The first droplet unit 2 comprises:

[0040] The dye solution storage cavity 202 arranged on the top, the dye solution filling port 201 arranged above the dye solution storage cavity 202, the micro-injection peristaltic pump 203 connected with the lower surface of the dye solution storage cavity 202 through a conduit, the dropper 204 connected with the output end of the micro-injection peristaltic pump 203, the first slide passing groove 205, and the visual camera 206 arranged below the height of the slide and used for capturing the dyeing state.

[0041] As shown in the utility model embodiment, Figure 1 The bottom surface of the dye solution storage cavity 202 is inclined along the side of the lower conduit port.

[0042] As Figure 3 shown in the embodiment of the utility model, the first cleaning unit 3, including:

[0043] The clean water storage cavity 302 is arranged on the top layer of the cylindrical shell 1, the clean water filling port 301 is arranged on the top surface of the clean water storage cavity 302, the water pump 303 is connected to the bottom of the clean water storage cavity 302 through a conduit, the spray head 304 is connected with the output port of the water pump 303, the seepage support plate 305 is arranged below the height of the slide, and the second slide groove 306 is arranged on the side of the first cleaning unit 3.

[0044] The drainage groove is below the seepage support plate 305.

[0045] As Figure 3 shown in the embodiment of the utility model, the first cleaning unit 3, including:

[0046] The second drainage rim 308 is arranged on the upper end of the inner side of the second slide groove 306 in the downward direction, and the first drainage rim 307 is arranged on the lower end of the outer side of the second slide groove 306 in the upward direction.

[0047] As Figure 4 shown in the embodiment of the utility model, the slide drying unit 6, including:

[0048] The first heating sheet 601 is arranged on the top layer of the cylindrical shell 1, the air blower 602 is arranged below the first heating sheet 601, the porous support plate 603 is arranged below the air blower 602 and is higher than the height of the slide, the second heating sheet 604 is arranged below the height of the slide, and the third slide groove 605 is arranged on the side of the slide drying unit 6.

[0049] The working principle of the embodiment of the utility model is as follows: first, the slide is clamped to the work position conversion unit 7, the slide is clamped to the slide groove 705 on the other side of the slide chuck 704 by controlling the directional rotation of the conversion motor 701, and the slide is clamped to the slide groove 705 on the other side of the slide chuck 704 by controlling the directional rotation of the conversion motor 701, and the slide is clamped to the fourth slide groove 706; then, the slide is sequentially brought into the above-mentioned work position, under the action of the micro-injection peristaltic pump 203, the first drop unit 2 drops phenol red solution to the slide, and the visual camera 206 assists to ensure the dyeing effect; then, under the action of the water pump 303, the first cleaning unit 3 washes the slide with clean water; then, the second drop unit 4 drops hydrochloric acid alcohol to the slide, then the second cleaning unit 5 washes the slide with clean water, the third drop unit 8 drops methylene blue to the slide, then the third cleaning unit 9 washes the slide with clean water, and finally the slide drying unit 6 blows dry.

[0050] In the embodiment of the utility model, based on standard operation process, through the drop, cleaning, blow dry station, orderly continuous around type station layout, and with station conversion unit 7 is located in the ring center, through the control conversion motor rotation, can realize the flexible continuous switching between multi-station, realize the integration and automation processing of primary dyeing, decoloring, re-dyeing process, replace manual operation, efficiency is higher, in addition, through the peristaltic pump control liquid adding amount, thereby solve the technical problem of the existing technology that liquid adding amount is not accurate and can easily cause the dye on the slide to overflow or dry, influence the technical problem of dye quality.

[0051] In addition, the heating sheet is used to replace the flame heating method, which not only realizes the temperature control, but also solves the technical problems that the flame heating method may induce fire or trigger the smoke sensor.

[0052] The above is only the preferred embodiment of the present application, and does not limit the present application. Any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application. The above is only the preferred embodiment of the present application, and it should be pointed out that, for ordinary skilled in the art, without departing from the technical principles of the present application, a number of improvements and modifications can be made, which should be regarded as the protection scope of the present application.

Claims

1. An automatic acid-fast staining apparatus for mycobacterium detection slide, characterized by, It includes: The cylindrical shell (1), the station conversion unit (7) arranged in the center of the cylindrical shell (1), the first droplet unit (2), the first cleaning unit (3), the second droplet unit (4), the second cleaning unit (5), the third droplet unit (8), the third cleaning unit (9) and the slide drying unit (6) arranged in sequence around the station conversion unit (7); The slide taking and placing area between the first droplet unit (2) and the slide drying unit (6); The first droplet unit (2), the second droplet unit (4) and the third droplet unit (8) are the same structure, and the first cleaning unit (3), the second cleaning unit (5) and the third cleaning unit (9) are the same structure; By clamping the slide to the station conversion unit (7), the slide is sequentially taken into the above station, the first droplet unit (2) drops phenol complex red solution on the slide, then the first cleaning unit (3) washes with water, the second droplet unit (4) drops hydrochloric acid alcohol on the slide, then the second cleaning unit (5) washes with water, the third droplet unit (8) drops methylene blue on the slide, then the third cleaning unit (9) washes with water, and finally the slide drying unit (6) blows dry.

2. The automatic acid-fast staining device for Mycobacterium detection slide according to claim 1, characterized in that, The station conversion unit (7) comprises: The conversion motor (701) arranged below the center of the cylindrical shell (1), the intermediate turntable (703) fixedly connected with the output shaft of the conversion motor (701) through the vertical rotating shaft (702), the slide chuck (704) arranged on the side of the intermediate turntable (703) and clamping the slide, the support slide groove (705) formed by cutting through the annular inside wall of the cylindrical shell (1), and the fourth slide groove (706) opened on the side of the slide taking and placing area of the cylindrical shell (1) to facilitate the slide to pass through. By controlling the directional rotation of the conversion motor (701), the other side of the slide clamped by the slide chuck (704) is clamped into the support slide groove (705) for support, and passes through the fourth slide groove (706).

3. The automatic acid-fast staining device for Mycobacterium detection slide according to claim 1, characterized in that, The first droplet unit (2) comprises: The dye storage cavity (202) arranged on the top, the dye filling port (201) arranged above the dye storage cavity (202), the micro-injection peristaltic pump (203) connected with the lower surface of the dye storage cavity (202) through the conduit, the dropper (204) connected with the output end of the micro-injection peristaltic pump (203), the first slide groove (205), and the visual camera (206) arranged below the height of the slide to capture the dyeing state.

4. The automatic acid-fast staining device for mycobacterium detection slide according to claim 3, characterized in that, The bottom surface of the dye storage cavity (202) is inclined along the side of the lower conduit port.

5. The automatic acid-fast staining device for Mycobacterium detection slide according to claim 1, wherein, The first cleaning unit (3) comprises: The clean water storage cavity (302) is arranged on the top layer of the cylindrical shell (1), the clean water filling port (301) is arranged on the top surface of the clean water storage cavity (302), the water pump (303) is connected to the bottom of the clean water storage cavity (302) through a conduit, the spray head (304) is connected with the output port of the water pump (303), the seepage support plate (305) is arranged below the height of the glass sheet, the second glass sheet passing groove (306) is arranged on the side of the first cleaning unit (3), and the drainage groove is arranged below the seepage support plate (305).

6. The automatic acid-fast staining device for Mycobacterium detection slide according to claim 5, wherein, The first cleaning unit (3) comprises: The second drainage edge (308) is arranged on the upper end of the inner side of the second glass sheet passing groove (306) in the downward direction, and the first drainage edge (307) is arranged on the lower end of the outer side of the second glass sheet passing groove (306) in the upward direction.

7. The apparatus for automated acid-fast staining of mycobacterial detection slides according to any one of claims 1 to 6, wherein, The glass sheet drying unit (6) comprises: The first heating sheet (601) is arranged on the top layer of the cylindrical shell (1), the air blower (602) is arranged below the first heating sheet (601), the porous support plate (603) is arranged below the air blower (602) and is higher than the height of the glass sheet, the second heating sheet (604) is arranged below the height of the glass sheet, and the third glass sheet passing groove (605) is arranged on the side of the glass sheet drying unit (6).

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