Cell counting plate capable of counting in batches

Abstract

The utility model discloses a cell counting plate capable of counting in batches. The cell counting plate comprises a bottom plate, a counting chamber, an overflow groove, a supporting plate and a cover plate, the counting chambers are arranged at the top of the bottom plate and are orderly arranged into two rows to form a counting chamber array, the counting chamber array comprises a downward hollowed-out self-flowing chamber, a counting table is arranged in the self-flowing chamber, and the counting table is surrounded by the self-flowing chamber; the overflow groove is arranged around the counting chamber; the supporting plates are arranged on the two sides of the counting chamber, and the top faces of the supporting plates are slightly higher than the top face of the counting table. The cover plate is arranged on the supporting plate, and the cover plate and the supporting plate are fixedly connected. A plurality of counting chambers are connected into a whole through a bottom plate and share one cover plate, different cell mixed liquid samples are added into each counting chamber, and target cells in each sample are counted in a counting table, so that counting of a plurality of samples is completed by using one counting plate, and rapid, efficient and accurate cell counting is realized.

Description

TECHNICAL FIELD

[0001] The embodiment of the utility model belongs to cell detection technical field, more particularly, relate to a cell counting plate of batch counting. BACKGROUND

[0002] As a classic tool in the field of blood cell counting, Neubauer hemocytometer has played an important role in clinical testing, biological research and drug development since its inception in the early 20th century, thanks to its precise etched grid structure and standardized counting method. Its core principle is to observe the number of blood cells in a specific grid area through a microscope, and combine dilution factor and volume conversion to realize quantitative analysis of cell concentration. However, with the rapid development of modern medical detection towards high-throughput and automation, the limitations of traditional Neubauer hemocytometer in technical architecture are increasingly prominent, especially in the batch processing of multiple samples. The traditional Neubauer hemocytometer adopts a single counting chamber design, which can only carry one sample at a time. The operator needs to complete the sample loading, standing settlement, microscope observation and cleaning steps one by one, and then proceed to the next sample detection. In clinical testing or large-scale experiments that require continuous processing of dozens or even hundreds of samples, repetitive operations significantly reduce work efficiency, and frequent human intervention can introduce operation errors.

[0003] A cell counting plate is disclosed in Chinese Patent No. CN221895003U, which comprises a glass bottom plate, a plurality of guide grooves are formed on the bottom surface of the glass bottom plate, a convex assembly is fixedly connected to the edges of both sides of the glass bottom plate, a support column is fixedly connected to the surface of the glass bottom plate, two counting pools are arranged in the middle of the glass bottom plate, and a plurality of guide grooves are formed on the top surface of the glass bottom plate. The cell counting plate is provided with a reinforcing assembly, which can effectively reduce the transmission of light to the minimum limit and reflect more light to the detection area, thereby increasing the clarity of the cells.

[0004] The cell counting plate comprises a plurality of counting grids arranged side by side, which can simultaneously count the cells of different samples. However, there is no isolation structure between the two counting grids, which can cause sample cross-contamination between the two adjacent counting grids, affecting the counting result. Therefore, there is a need for a cell counting plate that can batch count, realize rapid, efficient and accurate cell counting. UTILITY MODEL CONTENT

[0005] To address the aforementioned deficiencies or improvement needs of existing technologies, this invention provides a cell counting plate capable of batch counting. Multiple counting cells are connected as a whole by a base plate and share a single cover plate. Different cell mixture samples are added to each counting cell, and the target cells in each sample are counted separately in the counting stage. This allows multiple samples to be counted using a single counting plate without causing sample cross-contamination between adjacent counting grids, achieving rapid, efficient, and accurate cell counting.

[0006] To achieve the above objectives, this utility model provides a cell counting plate capable of batch counting, comprising a base plate, a counting chamber, an overflow trough, a support plate, and a cover plate;

[0007] The counting pools are located on the top of the base plate and are arranged in two neat rows to form a counting pool array. The array includes a self-flowing pool with a downward hollowed-out design. A counting platform is provided in the self-flowing pool, and the self-flowing pool surrounds the counting platform in the middle.

[0008] The overflow trough is located around the counting pool;

[0009] The support plate is located on both sides of the counting pool, and the top surface of the support plate is slightly higher than the top surface of the counting platform.

[0010] The cover plate is mounted on the support plate, and the two are fixedly connected.

[0011] Furthermore, the counting pool is also provided with a feed guide port;

[0012] The feed inlet is located on one side of the counting pool, and the feed inlets on both rows of counting pools are located on opposite sides.

[0013] The feed inlet is located at the contact point between the counting tank and the cover plate, and the feed inlet has a slanted opening that is smaller at the top and larger at the bottom.

[0014] Furthermore, a first guide column is provided in the self-flowing pool on the front of the counting platform, and a second guide column is provided in the self-flowing pool on the back of the counting platform.

[0015] Furthermore, the first guide column is located in the center of the front of the counting platform, and symmetrical inclined plates are provided on both sides.

[0016] Furthermore, the second guide column is located on both sides of the back of the counting platform, and each of them has an inclined plate facing one side of the counting platform.

[0017] Furthermore, a counting grid is provided at the corresponding position on the counting platform. The counting grid is divided into a nine-square grid, and the four corner squares in the nine-square grid are used as the first counting area.

[0018] Divide the center square of the 3x3 grid into several squares, with the four corner squares and the center square serving as the second counting area.

[0019] Further, the first counting area and the second counting area are each divided into sixteen counting grids.

[0020] Further, the overflow groove is separated from the self-flowing pool by an overflow plate, the top of the overflow plate is provided with an overflow guide opening inclined to the overflow groove, and the height of the overflow plate is between the counting table and the supporting plate.

[0021] Further, the middle of the bottom plate is provided with a light-transmitting pool corresponding to the counting table, and the light-transmitting pool is a hollow structure at the bottom of the bottom plate.

[0022] Further, the supporting plates on the adjacent two counting pools are further provided with a blocking groove.

[0023] Overall, compared with the prior art, the above technical scheme of the utility model can achieve the following beneficial effects:

[0024] 1. The cell counting plate of the utility model connects multiple counting pools into a whole through a bottom plate, shares a cover plate, adds different cell mixture samples in each counting pool, counts target cells in each sample in the counting table, realizes counting of multiple samples using one counting plate, and does not cause sample stringing between adjacent two counting grids, and realizes rapid, efficient and accurate cell counting.

[0025] 2. The cell counting plate of the utility model realizes double flow guiding of the cell mixture through the first flow guide column and the second flow guide column, so that the cell mixture rapidly fills the entire self-flowing pool and flows to the surface of the counting table.

[0026] 3. The cell counting plate of the utility model sets a light-transmitting pool with a hollow structure at the bottom of the bottom plate, provides light collection for the counting table, and facilitates observation of cells thereon.

[0027] 4. The cell counting plate of the utility model further provides a blocking groove between the supporting plates on the adjacent two counting pools, when the cell mixture sample is added, the sample accidentally dropped flows to the blocking groove, avoids aggregation of the sample on the cover plate, and avoids interference with counting of cells in other cell mixture samples, so as to cause inaccurate counting.

[0028] 5. The cell counting plate of the utility model, after the liquid level of the cell mixture in the self-flowing pool is higher than the top surface of the overflow plate, the cell mixture flows into the overflow groove through the overflow guide opening, and overflow of the cell mixture is avoided.

[0029] 6. The cell counting plate of the utility model is provided with a cover plate, can directly take out the sample for observation and counting when in use, omits the step of adding a cover glass, directly serves as waste after use, and makes operation more convenient and fast. BRIEF DESCRIPTION OF DRAWINGS

[0030] Figure 1 Structure top view of a cell counting plate capable of batch counting according to an embodiment of the present application;

[0031] Figure 2 Structure top view of a single counting pool in a cell counting plate capable of batch counting according to an embodiment of the present application;

[0032] Figure 3 Structure sectional view of a cell counting plate capable of batch counting according to an embodiment of the present application;

[0033] Figure 4 Structure sectional view of a single counting pool in a cell counting plate capable of batch counting according to an embodiment of the present application;

[0034] Figure 5 Counting grid structure schematic view of a cell counting plate capable of batch counting according to an embodiment of the present application.

[0035] In all the drawings, the same reference signs represent the same technical features, specifically: 1 - bottom plate, 2 - counting pool, 21 - self-flow pool, 22 - counting table, 23 - feeding flow guide port, 24 - first flow guide column, 25 - second flow guide column, 26 - counting grid, 261 - first counting area, 262 - second counting area, 3 - overflow groove, 31 - overflow plate, 32 - overflow flow guide port, 4 - support plate, 41 - limiting groove, 42 - guide groove, 5 - cover plate, 6 - light transmission pool, 7 - blocking groove. DETAILED DESCRIPTION

[0036] In order to make the purpose, technical scheme and advantages of the present application more clear, the present application will be further described in detail below with reference to the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and do not limit the present application. In addition, the technical features involved in each embodiment of the present application described below can be combined with each other as long as there is no conflict.

[0037] As Figures 1-5As shown, the utility model embodiment provides a kind of batchable cell counting plate, including bottom plate 1, be arranged at the top of the bottom plate 1 counting pool 2, be arranged at the periphery of the counting pool 2 overflow groove 3, be arranged at the both sides of the counting pool 2 support plate 4 and be arranged on the support plate 4 cover plate 5。Wherein, the top of the bottom plate 1 is arranged with two rows of counting pool 2 and forms counting pool array, the counting pool 2 includes the self-flowing pool 21 being hollowed out downwards, the self-flowing pool 21 is equipped with counting table 22, the self-flowing pool 21 is enclosed with counting table 22 in the middle.The top surface of the support plate 4 is slightly higher than the top surface of counting table 22, and the support plate 4 of both ends counting pool 2 end portion is extended to both ends, and the support plate 4 of two rows of counting pool 2 is connected integrally here.

[0038] The width of the cover plate 5 is same with the size around the counting pool 2, and the cover plate 5 is fixedly connected between the support plate 4.Through bottom plate 1, multiple counting pool 2 is connected as a whole, and cover plate 5 is fixed on each counting pool 2, different cell mixture sample is added in each counting pool 2, target cell in each sample is counted in counting table 22 respectively, multiple samples are counted using one counting plate, and sample string sample between adjacent two counting grids is not caused, fast, efficient and accurate cell counting is realized.

[0039] The feeding flow guide 23 is further provided in the counting pool 2, the feeding flow guide 23 is arranged at one side of the counting pool 2, and the feeding flow guide 23 on two rows of counting pool 2 is arranged at the side away from each other.The feeding flow guide 23 is arranged at the contact of counting pool 2 and cover plate 5, the feeding flow guide 23 is opened with top small and bottom large bevel at the top, and cell mixture enters into the self-flowing pool 21 through the feeding flow guide 23.

[0040] As further preferred, since the cell mixture flows into the self-flowing pool 21 from the feed flow guide port 23, the self-flowing pool 21 needs to be filled to reach the top surface of the counting table 22 for observation and counting, and the counting table 22 is located in the middle of the self-flowing pool 21 to better ensure the counting effect. During the process of filling the self-flowing pool 21, the cell mixture flows from the front to the back of the counting table 22, which is blocked by the counting table 22, resulting in a decrease in the flow rate of part of the cell mixture, thereby causing the deposition of cells, and the content of cells in the cell mixture is not uniform. Therefore, a first flow guide column 24 is arranged in the self-flowing pool 21 in front of the counting table 22, and a second flow guide column 25 is arranged in the self-flowing pool 21 behind the counting table 22. The first flow guide column 24 is arranged in the middle of the front of the counting table 22, and symmetrical inclined plates are arranged on both sides thereof. After the cell mixture flows into the self-flowing pool 21 from the feed flow guide port 23, it is divided into two parts by the first flow guide column 24, so that the cell mixture flows into the self-flowing pool 21 on both sides of the counting table 22 and smoothly flows to the back of the counting table 22. The second flow guide column 25 is arranged on both sides of the back of the counting table 22, and inclined plates are arranged on one side of the counting table 22. After the cell mixture flows into the self-flowing pool 21 on both sides of the counting table 22, it converges to the self-flowing pool 21 on the back of the counting table 22 by the flow guide effect of the second flow guide column 25. The cell mixture is double-flowed by the first flow guide column 24 and the second flow guide column 25, so that the cell mixture quickly fills the entire self-flowing pool 21 and flows to the surface of the counting table 22.

[0041] A counting grid 26 is also arranged at a corresponding position on the counting table 22. The counting grid 26 is divided into nine squares. The four corner squares in the nine squares are taken as a first counting area 261. The middle square in the nine squares is divided into 25 squares, and the four corner squares and the middle square are taken as a second counting area 262. The first counting area 261 is further divided into sixteen counting squares for counting large cells including white blood cells. The second counting area 262 is also divided into sixteen counting squares for counting small cells including red blood cells and platelets.

[0042] The overflow tank 3 and the self-flowing pool 21 are separated by an overflow plate 31. The top of the overflow plate 31 is provided with an overflow flow guide port 32 inclined to the overflow tank 3, and the height of the overflow plate 31 is between the counting table 22 and the support plate 4. After the liquid level of the cell mixture in the self-flowing pool 21 is higher than the top surface of the overflow plate 31, the cell mixture flows into the overflow tank 3 through the overflow flow guide port 32 to avoid overflowing.

[0043] As further preferred, a light transmission pool 6 is arranged at a corresponding position of the middle of the bottom plate 1 and the counting table 22. The light transmission pool 6 is a hollow structure arranged at the bottom of the bottom plate 1. The light transmission pool 6 provides light for the counting table 22, which is convenient for observing the cells on the counting table 22.

[0044] As further preferred, a barrier groove 7 is further provided between the support plates 4 on two adjacent counting chambers 2, when adding the cell mixture sample, the accidentally dropped sample flows to the barrier groove 7, avoiding its gathering on the cover plate 5, causing interference to the counting of cells in other cell mixture samples, thereby causing inaccurate counting.

[0045] As further preferred, the cell counting plate is made of high light transmission material.

[0046] Those skilled in the art can easily understand that the above description is only a preferred embodiment of the present application, and is not used to limit the present application, and any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A batch-countable cell counting plate, characterized by, It comprises a bottom plate (1), a counting pool (2), an overflow groove (3), a support plate (4) and a cover plate (5). The counting pool (2) is arranged on the top of the bottom plate (1) and is arranged in two rows to form a counting pool array, comprising a self-flowing pool (21) arranged in a hollow downward manner, wherein the self-flowing pool (21) is provided with a counting table (22), and the self-flowing pool (21) surrounds the counting table (22) in the middle. The overflow groove (3) is arranged around the counting pool (2). The support plate (4) is arranged on both sides of the counting pool (2), and the top surface of the support plate (4) is slightly higher than the top surface of the counting table (22). The cover plate (5) is arranged on the support plate (4) and is fixedly connected therebetween.

2. A batch countable cell counting plate according to claim 1, wherein, The counting pool (2) is further provided with a feeding flow guide opening (23). The feeding flow guide opening (23) is arranged on one side of the counting pool (2), and the feeding flow guide openings (23) on the two rows of counting pools (2) are arranged on sides away from each other. The feeding flow guide opening (23) is arranged at the contact position of the counting pool (2) and the cover plate (5), and a top small and bottom large inclined opening is formed at the feeding flow guide opening (23).

3. A batch countable cell counting plate according to claim 2, wherein, The first flow guide column (24) is further arranged in the self-flowing pool (21) in front of the counting table (22), and the second flow guide column (25) is further arranged in the self-flowing pool (21) behind the counting table (22).

4. A batch countable cell counting plate according to claim 3, wherein, The first flow guide column (24) is arranged at the middle of the front of the counting table (22), and symmetrical inclined plates are arranged on both sides thereof.

5. A batch countable cell counting plate according to claim 4, wherein, The second flow guide column (25) is arranged on both sides of the back of the counting table (22), and an inclined plate is arranged on one side of the counting table (22).

6. A batch-countable cell counting plate according to any one of claims 1-5, wherein, A counting grid (26) is further arranged on the counting table (22) at a corresponding position, the counting grid (26) is divided into nine grids, and the four corner grids in the nine grids are used as first counting areas (261). The middle grid in the nine grids is divided into 25 grids, and the four corner grids and the middle grid are used as second counting areas (262).

7. A batch countable cell counting plate according to claim 6, wherein, The first counting areas (261) and the second counting areas (262) are each divided into sixteen counting grids.

8. A batch-countable cell counting plate according to any one of claims 1-5, wherein, The overflow groove (3) and the self-flowing pool (21) are separated by an overflow plate (31), the top of the overflow plate (31) is provided with an overflow flow guide opening (32) inclined to the overflow groove (3), and the height of the overflow plate (31) is between the counting table (22) and the support plate (4).

9. A batch-countable cell counting plate according to any one of claims 1-5, wherein, A light transmission pool (6) is further arranged at a corresponding position of the counting table (22) in the middle of the bottom plate (1), and the light transmission pool (6) is arranged in a hollow manner at the bottom of the bottom plate (1).

10. A batch-countable cell counting plate according to any one of claims 1-5, wherein, A blocking groove (7) is further arranged between the support plates (4) on two adjacent counting pools (2).

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