Portable electromagnetic rod for purifying cells and nucleic acid

Abstract

The utility model discloses a portable electromagnetic rod for cell and nucleic acid purification, which comprises a handle, an assembly groove, a heat dissipation aluminum sleeve and a skeleton, the middle part of the inner side of the handle is provided with the assembly groove for component assembly, the lower end of the handle is fixedly provided with the heat dissipation aluminum sleeve for heat dissipation protection, the handle is embedded with the skeleton along the middle part of the assembly groove, and the skeleton is embedded with the heat dissipation aluminum sleeve. A conical iron core is fixed to the middle of the inner side of the framework, and an electromagnetic coil is wound around the outer wall of the conical iron core. When the portable electromagnetic rod for cell and nucleic acid purification is used, a magnetic field is generated and eliminated by controlling the on-off of the current of the battery, so that the adsorption and release operations of magnetic beads are realized. The device is simple in structure, low in cost and convenient to carry, is suitable for separation and purification of cells and nucleic acid in various laboratory environments, and is also suitable for centrifugal tubes and 96-well plates with different specifications. The shape design imitates a pipette, so that the operation process is more natural and convenient, and a user can complete the whole operation process with one hand.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell, nucleic acid separation and purification device technical field, concretely for a kind of portable electromagnetic rod for cell and nucleic acid purification. BACKGROUND

[0002] In modern cell biology research, in order to reveal the spatial localization of biological macromolecules, the relationship and function, it is crucial to separate and purify cells with high purity from in vitro cultured cells quickly and efficiently. Currently, cell separation often uses magnetic bead labeling technology, which uses specific antibodies on the surface of magnetic beads to label target cells and separates target cells from samples through an external magnetic field.

[0003] However, permanent magnets are commonly used in the prior art, which have fixed magnetic field strength and direction that cannot be adjusted, making it lack flexibility when adsorbing magnetic beads and often requiring additional mechanical operations (such as vibration or scraping) when releasing magnetic beads, which may cause sample loss or incomplete purification. In addition, in containers with conical bottoms such as centrifuge tubes, the magnetic field of traditional magnets cannot completely cover the target area, resulting in low adsorption efficiency. In small-volume containers such as 96-well plates, the strong magnetic field of permanent magnets may cause excessive adsorption or limited operation, making it unsuitable for high-precision experiments. Therefore, we propose a portable electromagnetic rod for cell and nucleic acid purification, which can flexibly control the adjustable magnetic field to overcome the shortcomings of traditional methods in adsorption, release, and compatibility with specific containers. SUMMARY

[0004] The utility model aims at providing a portable electromagnetic rod for cell and nucleic acid purification to solve the problems raised in the background.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a portable electromagnetic rod for cell and nucleic acid purification, comprising a handle, an assembly slot, a heat dissipation aluminum sleeve, and a skeleton. The inner side of the handle is provided with an assembly slot for component assembly. The lower end of the handle is fixed with a heat dissipation aluminum sleeve for heat dissipation protection. The handle is embedded with a skeleton along the middle part of the assembly slot. The inner side of the skeleton is fixed with a conical iron core. The outer wall of the conical iron core is wrapped with an electromagnetic coil. The connection between the conical iron core and the inner wall of the handle is sleeved with a limiting sleeve. The end of the conical iron core is provided with a magnetic attraction probe for magnetic ball adsorption.

[0006] Preferably, the upper end of the handle is hot-melted with a finger sleeve hook on one side, and the surface of the finger sleeve hook is arc-shaped.

[0007] Preferably, the outer wall surface of the handle is embedded with an LED display screen with display function, and the outer wall of the handle is embedded with a buzzer with buzzer prompting function above the LED display screen.

[0008] Preferably, the conical iron core is attached with a temperature sensor with temperature detection function on one side of the lower end of the handle, and the temperature sensor is electrically connected with the LED display screen and the buzzer respectively.

[0009] Preferably, the number of turns of the electromagnetic coil is 450-550 turns, and the magnetic field strength of the electromagnetic coil is 300-500 millitesla.

[0010] Preferably, the lower end of the conical iron core penetrates into the heat dissipation aluminum sleeve, and the end of the conical iron core penetrates out of the heat dissipation aluminum sleeve.

[0011] Preferably, the handle is arranged with a lithium battery providing power above the framework, and the handle is arranged with a control button controlling the switch of the control circuit above the lithium battery.

[0012] Preferably, the assembly groove is sequentially assembled with the control button, the lithium battery and the framework from top to bottom, and the reset spring is installed at the connection between the control button and the assembly groove.

[0013] Compared with the prior art, the portable electromagnetic rod for cell and nucleic acid purification has the advantages that: when the portable electromagnetic rod for cell and nucleic acid purification is used, the on-off of the battery current is controlled to generate and eliminate the magnetic field, so that the adsorption and release operations of the magnetic beads are realized. The portable electromagnetic rod for cell and nucleic acid purification has the advantages of simple structure, low cost and portability, is suitable for cell and nucleic acid separation and purification in various laboratory environments, and is suitable for different specifications of centrifugal tubes and 96-hole plates. The appearance design imitates the pipette gun, so that the operation process is more natural and convenient, and the user can complete the entire operation process with one hand.

[0014] The magnetic attraction probe 64 is externally covered with the high-efficiency heat dissipation aluminum sleeve 5, which is beneficial to quickly dissipate heat from the probe area. The probe part is designed with multiple ring-shaped heat dissipation holes, and a small air duct structure is built inside the heat dissipation aluminum sleeve 5, so as to naturally convect heat during work and avoid heat accumulation. These heat dissipation holes are evenly distributed to ensure that the temperature of the entire probe part is uniform and prevent local overheating.

[0015] The magnetic attraction probe part is made of high-magnetic-conductivity iron material, and the length is about 5 cm. The front end is about 1 cm of effective adsorption area. The tip of the magnetic attraction probe is designed in a conical shape to maximize the concentration of the magnetic field and ensure efficient adsorption of the magnetic beads. BRIEF DESCRIPTION OF DRAWINGS

[0016] Fig. 1 It is a three-dimensional structure schematic diagram of the utility model;

[0017] Fig. 2 It is an internal structure schematic diagram of the utility model;

[0018] Fig. 3 It is a framework structure schematic diagram of the utility model.

[0019] In the figure: 1, handle; 11, finger sleeve hook; 2, assembly groove; 3, LED display screen; 4, buzzer; 5, heat dissipation aluminum sleeve; 6, skeleton; 61, electromagnetic coil; 62, limiting sleeve; 63, conical iron core; 64, magnetic attraction probe; 7, lithium battery; 8, temperature sensor; 9, control button; 91, reset spring. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0021] Please refer to Figs. 1-3 The present application provides a technical solution: a portable electromagnetic rod for cell and nucleic acid purification, comprising a handle 1, an assembly groove 2, a heat dissipation aluminum sleeve 5 and a skeleton 6. The inner side of the middle part of the handle 1 is provided with an assembly groove 2 for component assembly. The lower end of the handle 1 is fixed with a heat dissipation aluminum sleeve 5 for heat dissipation protection. The middle part of the handle 1 along the assembly groove 2 is embedded with a skeleton 6. The inner side of the middle part of the skeleton 6 is fixed with a conical iron core 63. The outer wall of the conical iron core 63 is wound with an electromagnetic coil 61. The connection between the conical iron core 63 and the inner wall of the handle 1 is sleeved with a limiting sleeve 62. The end of the conical iron core 63 is provided with a magnetic attraction probe 64 for magnetic ball adsorption. The upper end of the handle 1 is hot-melted with a finger sleeve hook 11 on one side. The surface of the finger sleeve hook 11 is distributed in a circular arc shape. The entire handle 1 and the finger sleeve hook 11 are designed with ergonomics. The overall shape imitates a pipette. The outer layer of the handle is wrapped with a non-slip rubber coating to ensure the comfort and stability during long-time operation.

[0022] The outer wall surface of the handle 1 is embedded with an LED display screen 3 with display function, and the outer wall of the handle 1 is embedded with a buzzer 4 with buzzer prompting function above the LED display screen 3, and the lower end of the cone-shaped core 63 is attached with a temperature sensor 8 with temperature detection function, and the temperature sensor 8 is electrically connected with the LED display screen 3 and the buzzer 4 respectively. When the temperature of the magnetic attraction probe 64 approaches the upper limit set, for example, 40 degrees Celsius, the temperature sensor 8 will trigger the buzzer 4 on the handle to emit a prompt sound, and at the same time the LED display screen 3 will flash an orange warning signal, prompting the user to take measures such as pausing the operation or moving the device away from the sample. In order to avoid the magnetic beads from falling due to automatic power-off, the device will not automatically power off when the temperature exceeds the standard. On the contrary, the prompting system aims to guide the user to handle it in time to ensure the continuity of the separation process and the stability of the purification effect. The prompt sound of the buzzer is designed as intermittent "ding-ding" sound with moderate volume, which will not interfere with other operating environments in the laboratory. At the same time, the flashing frequency of the indicator light gradually increases, indicating that the temperature is further rising, providing multiple warning means to ensure that the operator can pay attention in time.

[0023] The number of turns of the electromagnetic coil 61 is 450-550 turns, and the magnetic field strength of the electromagnetic coil 61 is 300-500 millitesla. The magnetic field strength of the electromagnetic coil 61 is designed to be 300-500 millitesla, which is sufficient to attract magnetic beads with a diameter of 1-5 microns. The electromagnetic coil 61 is arranged in the middle position of the handle and is wound with 500 turns of high-quality copper wire. The electromagnetic coil 61 is guided by the cone-shaped core 63 to concentrate the magnetic field to the magnetic attraction probe 64 part. The power of the electromagnetic coil 61 is optimized to ensure that the magnetic field is generated efficiently while reducing the risk of excessive heating. A waterproof control button 9 is provided on the handle for controlling the on-off of the electromagnetic coil 61. The control button 9 is designed to be located in an area where the finger can naturally contact, ensuring that the user does not need to make additional adjustments when using, improving the overall operation convenience. The magnetic attraction probe 64 part is made of high magnetic permeability iron material, with a length of about 5 centimeters and an effective adsorption area of about 1 centimeter at the front end. The tip of the magnetic attraction probe 64 is designed in a conical shape to maximize the concentration of the magnetic field and ensure efficient adsorption of magnetic beads.

[0024] The lower end of the cone-shaped core 63 penetrates into the heat dissipation aluminum sleeve 5, and the end of the cone-shaped core 63 penetrates out of the heat dissipation aluminum sleeve 5. The magnetic attraction probe 64 is covered with a high-efficiency heat dissipation aluminum sleeve 5, which is beneficial to quickly dissipate heat from the probe area. The probe part is designed with multiple ring-shaped heat dissipation holes and a small air duct structure inside the heat dissipation aluminum sleeve 5 to facilitate natural convection cooling during work and avoid heat accumulation. These heat dissipation holes are evenly distributed to ensure that the temperature of the entire probe part is uniform and prevent local overheating.

[0025] The handle 1 is provided with a lithium battery 7 for power supply above the skeleton 6, and a control button 9 for controlling the circuit switch above the lithium battery 7. The electromagnetic rod uses a rechargeable lithium battery with a capacity of 4000-5000mAh. The battery is built into the lower half of the handle to ensure even weight distribution of the device and prevent the user from feeling heavy-headed during operation. The assembly groove 2 is sequentially assembled with the control button 9, the lithium battery 7 and the skeleton 6 from top to bottom, and a reset spring 91 is installed at the connection between the control button 9 and the assembly groove 2.

[0026] Working principle: For this kind of portable electromagnetic rod for cell and nucleic acid purification, first hold the handle 1, the finger sleeve hook 11 presses the upper part of the index finger, and the thumb presses the control button 9. At this time, the user aims at the sample and presses the control button 9. The entire electromagnetic coil 61 is electrified, the current passes through the electromagnetic coil 61 to generate a magnetic field, the magnetic field is concentrated to the tip of the magnetic attraction probe 64 through the lead conical iron core 63, and a magnetic field with sufficient intensity is generated to adsorb the magnetic beads in the sample. Then the user releases the control button 9, the electromagnetic field disappears quickly, the magnetic beads fall off the probe, and at the same time, when the temperature of the magnetic attraction probe 64 approaches the set upper limit, for example, 45 degrees Celsius, the temperature sensor 8 triggers the buzzer 4 on the handle to emit a prompt sound, and the LED display screen 3 indicator light flashes an orange warning signal, reminding the user to take measures such as pausing the operation or moving the device away from the sample. Finally, the user repeats the operation of the electromagnetic rod to separate and purify the cells and nucleic acids.

Claims

1. A portable electromagnetic rod for cell and nucleic acid purification, comprising a handle (1), an assembly slot (2), a heat dissipation aluminum sleeve (5) and a skeleton (6), characterized in that: The inner middle part of the handle (1) is provided with an assembly slot (2) for assembling parts, the lower end of the handle (1) is fixed with a heat dissipation aluminum sleeve (5) for heat dissipation protection, the handle (1) is embedded with a framework (6) along the middle part of the assembly slot (2), and the inner middle part of the framework (6) is fixed with a conical iron core (63), the outer wall of the conical iron core (63) is wound with an electromagnetic coil (61), and the conical iron core (63) is connected with the inner wall of the handle (1) and is sleeved with a limiting sleeve (62), and the end of the conical iron core (63) is provided with a magnetic attraction probe (64) for attracting magnetic balls.

2. A portable electromagnetic rod for cell and nucleic acid purification according to claim 1, characterized in that: The upper end of the handle (1) is hot-melted with a finger sleeve hook (11) on one side, and the surface of the finger sleeve hook (11) is distributed in a circular arc shape.

3. A portable electromagnetic rod for cell and nucleic acid purification according to claim 1, characterized in that: The outer wall surface of the handle (1) is embedded with an LED display screen (3) with display function, and the outer wall of the handle (1) is embedded with a buzzer (4) with buzzer prompting function above the LED display screen (3).

4. A portable electromagnetic rod for cell and nucleic acid purification according to claim 3, characterized in that: The conical iron core (63) is attached with a temperature sensor (8) with temperature detection function on one side of the lower end of the handle (1), and the temperature sensor (8) is electrically connected with the LED display screen (3) and the buzzer (4) respectively.

5. A portable electromagnetic rod for cell and nucleic acid purification according to claim 1, characterized in that: The number of turns of the electromagnetic coil (61) is 450-550 turns, and the magnetic field strength of the electromagnetic coil (61) is 300-500 millitesla.

6. A portable electromagnetic rod for cell and nucleic acid purification according to claim 1, characterized in that: The lower end of the conical iron core (63) penetrates into the heat dissipation aluminum sleeve (5), and the end of the conical iron core (63) penetrates out of the heat dissipation aluminum sleeve (5).

7. A portable electromagnetic rod for cell and nucleic acid purification according to claim 1, characterized in that: The handle (1) is provided with a lithium battery (7) for providing power above the framework (6), and the handle (1) is provided with a control button (9) for controlling the circuit switch above the lithium battery (7).

8. A portable electromagnetic rod for cell and nucleic acid purification according to claim 7, characterized in that: The assembly slot (2) is sequentially assembled with the control button (9), the lithium battery (7) and the framework (6) from top to bottom, and the reset spring (91) is installed at the connection between the control button (9) and the assembly slot (2).

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