Separation and collection device for intestinal microorganisms

Abstract

The utility model discloses an intestinal microorganism separating and collecting device, and relates to the technical field of biomedicine. The device comprises a filtering and impurity removing box, a first partition plate and a second partition plate, the first partition plate divides the interior of the filtering and impurity removing box into an operation area and a background area, and the second partition plate divides the operation area into a clean area and a polluted area; a crushing assembly for crushing the excrement sample and a filtering assembly for filtering the crushed mixture are arranged in the pollution area; a dilution bottle is arranged in the clean area; a negative pressure assembly used for conveying a water source in the dilution bottle into the material crushing assembly and the filtering assembly in sequence is arranged in the background area. The cleaning device is low in cleaning difficulty and convenient to maintain.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biomedical technology, concretely relates to an intestinal microorganism separation and collection device. BACKGROUND

[0002] At present, it is a great challenge for medical staff to separate intestinal microorganisms (including intestinal flora) from feces, and how to realize the separation and collection of intestinal microorganisms is of great significance for medical staff, which can lay a solid foundation for precise transplantation of intestinal flora and precise treatment of intestinal diseases related to specific diseases.

[0003] The existing intestinal microorganism separation and collection device mainly filters and removes impurities of feces to separate and collect intestinal microorganisms from feces, which mainly has the following two implementation ways: the first way is to use a multi-stage filtering device, and domestic patent reports such as CN105624027B, CN105624024B, CN108676704B, CN209052694U and CN306895532S etc.; the second way is to use a nuclear pore membrane with different pore sizes, and domestic patent reports such as CN108949631A, CN108676704A etc.

[0004] The above two filtering and impurity removing devices have the problem that electronic components, pollution area and clean area are located on the same working surface, so that the cleaning difficulty is high and the maintenance difficulty is large, therefore, it is necessary to improve it. UTILITY MODEL CONTENT

[0005] The utility model aims at overcoming the defects of the prior art, and provides an intestinal microorganism separation and collection device, which has low cleaning difficulty and small maintenance difficulty.

[0006] In order to achieve the above-mentioned purpose, the technical scheme designed by the utility model is as follows:

[0007] An intestinal microorganism separation and collection device, comprising a filtering and impurity removing box, a first partition plate and a second partition plate; the first partition plate divides the filtering and impurity removing box into an operation area and a backstage area, and the second partition plate divides the operation area into a clean area and a pollution area; a material crushing assembly for crushing fecal samples and a filtering assembly for filtering the mixture after crushing are arranged in the pollution area; a dilution bottle is arranged in the clean area; and a negative pressure assembly for sequentially conveying water in the dilution bottle into the material crushing assembly and the filtering assembly is arranged on the first partition plate.

[0008] In the above technical scheme, the material crushing assembly comprises a sample tank and a material crushing motor, a material crushing shaft with a material crushing blade is arranged in the sample tank, and the output end of the material crushing motor is connected with the material crushing shaft and drives the material crushing shaft to rotate.

[0009] In the technical scheme, the filter assembly comprises a primary filter for performing primary filtering on the mixture after being crushed and a secondary filter for performing secondary filtering on the mixture after being crushed, and the dilution bottle, the sample jar, the primary filter and the secondary filter are sequentially connected by pipelines.

[0010] In the technical scheme, the pollution area is provided with a first support plate for placing the sample jar and a third support plate installed on the first support plate and used for supporting the first support plate, the crushing motor is installed below the first support plate, a crushing coupling is arranged in the third support plate, and the output end of the crushing motor is connected with the crushing shaft through the crushing coupling.

[0011] In the technical scheme, the pollution area is provided with a second support plate for placing the primary filter and the secondary filter, the first support plate is located below the second support plate, and the first support plate and the second support plate are connected through a fourth support plate.

[0012] In the technical scheme, the negative pressure assembly comprises a first peristaltic pump installed between the dilution bottle and the sample jar and a second peristaltic pump installed between the primary filter and the secondary filter.

[0013] In the technical scheme, the application further comprises an odor purification bottle and a negative pressure pump, one end of the negative pressure pump is in communication with the odor purification bottle and the crushing assembly in sequence, and the other end is in communication with a buffer and a filter in sequence.

[0014] In the technical scheme, the buffer comprises a first buffer bottle and a second buffer bottle arranged in sequence along the flow direction of the gas, a first control valve is arranged between the odor purification bottle and the sample jar, and a second control valve is arranged between the filter and the bacterial liquid collection bottle.

[0015] In the technical scheme, the pollution area is further provided with a sample collection rack, a plurality of bacterial liquid collection bottles are installed on the sample collection rack, and the bacterial liquid collection bottles are used for collecting the mixture treated by the filter assembly.

[0016] In the technical scheme, the shell of the clean area is further provided with a display controller, and the crushing assembly, the filter assembly and the negative pressure assembly are electrically connected with the display controller.

[0017] In the technical scheme, the filter and impurity removal box is connected with a filter workbench at the lower end, the filter workbench comprises a nitrogen tank for placing nitrogen and a consumable tank for placing consumables, and the nitrogen tank is located between the filter and impurity removal box and the consumable tank.

[0018] In the above technical solution, the filter impurity removal box is further provided with one or more of a printer, a fan, a buzzer, a spotlight and an illuminating lamp.

[0019] The present application has the following beneficial effects:

[0020] 1. The present application can separate intestinal microorganisms from fecal samples. Under the joint action of the first and second partitions, when it is necessary to replace sampling consumables (including the crushed material assembly, the filter assembly, the sample collection rack and the bacterial liquid collection bottle), the present application only needs to open the first cover plate. When it is necessary to supplement or replace the diluent, the present application only needs to open the second cover plate. In this way, the pollution area, the clean area and the background area cooperate with each other and do not interfere with each other, thereby avoiding cross contamination and making the present application more convenient to use.

[0021] 2. The present application is more compact and reasonable in layout, and more convenient to use, by setting the first, second, third and fourth support plates.

[0022] 3. The present application can automatically collect intestinal microorganisms from fecal samples, is safe and reliable, and has high work efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 The present application is a schematic structural view of the intestinal microorganism separation and collection device.

[0024] Figure 2 The present application is a schematic structural view of the filter impurity removal box when the first and second cover plates are opened.

[0025] Figure 3 The present application is Figure 2 The front view without the first and second cover plates.

[0026] Figure 4 The present application is Figure 2 The rear view without the first and second cover plates.

[0027] Figure 5 The present application is a half-section schematic structural view of the primary or secondary filter.

[0028] Figure 6 The present application is a flowchart.

[0029] In the figure: 100, impurity removal device; 101, printer; 102, fan; 103, buzzer; 104, spotlight; 105, illuminating lamp; 106, first cover plate; 107, second cover plate; 108, impurity removal box; 111, operation area; 112, clean area; 113, contaminated area; 101, background area; 121, first partition; 122, second partition; 123, first support plate; 124, second support plate; 125, third support plate; 126, fourth support plate; 130, crushing assembly; 131, sample jar; 132, crushing motor; 133, crushing coupling; 140, filtering assembly; 141, primary filter; 142, secondary filter; 150, dilution bottle; 160, negative pressure assembly; 161, first peristaltic pump; 162, second peristaltic pump; 171, odor purification bottle; 172, negative pressure pump; 173, buffer; 1731, first buffer bottle; 1732, second buffer bottle; 174, filter; 175, first control valve; 1732, second control valve; 181, bacterial liquid collection rack; 182, bacterial liquid collection bottle; 191, display controller; 192, nitrogen cabinet; 193, consumable cabinet. DETAILED DESCRIPTION

[0030] The utility model will be described in further detail below in combination with specific embodiments, so that those skilled in the art can understand.

[0031] The interaction between human beings and microorganisms is extremely close. Soon after the birth of a newborn, a large number of external microorganisms begin to adhere to its body surface and intestinal tract, most of which are adapted to the human body and eventually form a symbiotic relationship with the human body throughout life. Among these symbiotic microorganisms, about 100 trillion are colonized in the human intestinal tract, covering various microorganisms such as bacteria, fungi, viruses, etc. The intestinal microorganism is the most complex and largest microecological system in the human body, and is known as the eighth organ of the human body. The genome of intestinal microorganism contains about 5 million genes, about 150 times the number of human genes, and due to its large number and rich species characteristics, intestinal microorganism is called the "second gene bank" of the human body.

[0032] Intestinal microorganisms can produce short-chain fatty acids (SCFAs) by fermenting human indigestible dietary fiber to provide energy for colon epithelial cells, regulate the interaction between innate immunity and adaptive immunity, and the host's defense mechanism against intestinal pathogens. A normal and stable intestinal microorganism plays a key role in body defense against infection, maintenance of normal intestinal barrier, immunity, metabolism, nutrition, and maintenance of internal environment homeostasis through interaction with multiple systems of the human body such as immunity, endocrine and nervous system.

[0033] Currently, fecal microbiota transplantation (FMT) technology is gradually attracting widespread attention. In 2023, the National Health Commission officially included FMT in the "Notice on the Issuance of National Medical Service Project Technical Specifications". As an innovative treatment method, FMT can restore intestinal microecological balance and provide a new approach to the treatment of various diseases.

[0034] FMT originated from the works "Elbow Post Emergency Prescription" in the Eastern Jin Dynasty and "Suwen" in the Spring and Autumn Period. Traditional Chinese medicine uses human feces (recorded as Huanglong Decoction / Golden Juice) to treat intestinal diseases. In 1958, surgeon Eiseman used human feces to treat patients with pseudomembranous enteritis. In 2013, the American Medical Guide announced that human feces could be used to treat Clostridium difficile.

[0035] Since then, FMT has shown significant efficacy in treating gastrointestinal diseases such as irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), recurrent Clostridium difficile infection (CDI), ulcerative colitis (UC), and functional constipation (FC). With the development of research and the continuous updating of technologies such as high-throughput sequencing, artificial intelligence big data analysis, and intelligent production systems, the number of clinical application cases has also increased, showing a broad application prospect.

[0036] Currently, for medical personnel, isolating intestinal microorganisms (including intestinal flora) from feces is a significant challenge. How to achieve the isolation and collection of intestinal microorganisms is of great significance to medical personnel, as it can lay a solid foundation for precise transplantation of intestinal flora and precise treatment of specific intestinal diseases.

[0037] Existing intestinal microorganism isolation and collection devices mainly filter and remove impurities from feces to isolate and collect intestinal microorganisms. There are mainly two implementation methods: the first method is to use a multi-stage filtration device, such as domestic patents CN105624027B, CN105624024B, CN108676704B, CN209052694U, and CN306895532S, etc.; the second method is to use a nuclear pore membrane with different pore sizes, such as CN108949631A and CN108676704A.

[0038] Both of the above filtration and impurity removal devices have the problem of electronic components, contaminated areas, and clean areas located on the same working surface, making them difficult to clean and maintain. Therefore, it is necessary to improve them.

[0039] To address the above problems, such as Figure 1As shown, the utility model provides a kind of intestinal microorganism separation collection device (also can be called filter impurity removal device 100), including filter impurity removal box 108, first partition 121 and second partition 122;First partition 121 divides the inside of filter impurity removal box 108 into operation area 111 and backstage area 114, and second partition 122 divides operation area 111 into clean area 112 and contaminated area 113.

[0040] Operation area 111 is mainly used to place those needing operating personnel to operate and frequently replaced parts, such as primary filter 141, secondary filter 142, sample tank 131, bacteria liquid collection bottle 182 and second peristaltic pump 162 etc.;Backstage area 114 is mainly used to place those needing operating personnel to operate parts, such as negative pressure pump 172, first buffer bottle 1731, second buffer bottle 1732 and first peristaltic pump 161 etc.;In this way, the present application also avoids the problem that electronic components, contaminated area 113 and clean area 112 are located on the same work surface, and workers only need to clean contaminated area 113 frequently, and occasionally clean clean area 112 and backstage area 114, which is lower in washing difficulty and maintenance difficulty.

[0041] In actual work, in order to make the present application more convenient to use, more clean, neat and sanitary, through hole can be provided on first partition 121 and / or second partition 122 to pass pipeline, which is a conventional means in the art, and will not be repeated here.

[0042] The present application forms slope (60-85 °, preferably 75 °) on the front end face of filter impurity removal box 108, so that workers can view the working condition in contaminated area 113 through the glass on first cover plate 106, and view the working condition in clean area 112 through the glass on second cover plate 107, and increase the aesthetic appearance of the present application.

[0043] Filter impurity removal box 108 is provided with printer 101, illuminating lamp 105, spotlight 104, socket and control cabinet etc. components, so as to make the present application more convenient to use;Further, fan 102 and buzzer 103 can also be provided in backstage area 114, fan 102 can be used to reduce the odor concentration in contaminated area 113, and buzzer 103 can be used for alarm, so as to make the present application more convenient to use.

[0044] In actual work, the above-mentioned printer 101, illuminating lamp 105, spotlight 104, socket, control cabinet, fan 102 and buzzer 103 are all conventional products in the art, and their installation mode and installation position are all conventional means in the art, which will not be repeated here.

[0045] The contaminated zone 113 is equipped with a shredding assembly 130 for shredding fecal samples and a filtration assembly 140 for filtering the mixture after shredding. The clean zone 112 is equipped with a dilution bottle 150. The backstage zone 114 is equipped with a negative pressure assembly 160 for sequentially conveying water from the dilution bottle 150 to the shredding assembly 130 and the filtration assembly 140.

[0046] With the combined action of the aforementioned fragmentation component 130, filter component 140, dilution bottle 150, and negative pressure component 160, this application can automatically separate intestinal microorganisms (including beneficial bacteria, harmful bacteria, fungi, viruses, and other microorganisms) from fecal samples. When it is necessary to replace the sampling consumables (fragmentation component 130 and filter component 140), users only need to open the first cover 106; when it is necessary to replenish or replace the diluent, this application only needs to open the second cover 118. In this way, the contaminated area 113, the clean area 112, and the back-end area 114 cooperate with each other without interfering with each other, which can avoid cross-contamination and make this application more convenient to use.

[0047] The shredding assembly 130 includes a sample container 131 and a shredding motor 132. The sample container 131 contains a shredding shaft with shredding blades. The output of the shredding motor 132 is connected to the shredding shaft and drives it to rotate. The sample container 131 is installed within the contaminated area 113. When the shredding motor 132 drives the shredding shaft to rotate, the shredding shaft drives the shredding blades to rotate, thereby shredding the sample.

[0048] The filtration assembly 140 includes a primary filter 141 for primary filtration of the mixture after crushing and a secondary filter 142 for secondary filtration of the mixture after crushing. The dilution bottle 150, sample container 131, primary filter 141, and secondary filter 142 are connected sequentially via tubing (not shown in the figure). Through the combined action of the primary filter 141 and secondary filter 142, this application achieves multiple filtration functions on the mixture after crushing, thereby ultimately yielding an intestinal microbial solution.

[0049] In actual work, such as Figure 5 As shown, both the primary filter 141 and the secondary filter 142 include a barrel body and a barrel cover. The filters are equipped with multiple (six) layers of filter screens, and spacers are placed between adjacent filter screens. The mesh size of the filter screens in the primary filter 141 and the secondary filter 142 is 40 to 500.

[0050] Preferably, the first supporting plate 123 is arranged in the pollution area 113, and a third supporting plate 125 for placing the sample jar 131 is arranged on the first supporting plate 123, the crushing motor 132 is arranged below the first supporting plate 123, the crushing coupling 133 is arranged in the third supporting plate 125, and the output end of the crushing motor 132 is connected with the crushing shaft through the crushing coupling 133. In this way, when experiments are performed on different fecal samples, the staff only need to replace the sample jar 131, so that the application is more convenient to use.

[0051] Further, the second supporting plate 124 for placing the primary filter 141 and the secondary filter 142 is arranged in the pollution area 113, the first supporting plate 123 is arranged below the second supporting plate 124, and the first supporting plate 123 and the second supporting plate 124 are connected through the fourth supporting plate 126 arranged at the bottom of the pollution area 113. In this way, the structure of the application is more compact, the sample jar 131, the primary filter 141 and the secondary filter 142 are arranged more reasonably in the pollution area 113, and the application is more convenient to use.

[0052] Further, the first supporting plate 123 and the second supporting plate 124 are horizontally arranged, the fourth supporting plate 126 is vertically arranged, the first supporting plate 123, the second supporting plate 124 and the fourth supporting plate 126 are connected with the first partition 121, and the fourth supporting plate 126 is arranged on the bottom plate of the pollution area 113.

[0053] The negative pressure assembly 160 includes the first peristaltic pump 161 arranged between the dilution bottle 150 and the sample jar 131, and the second peristaltic pump 162 arranged between the primary filter 141 and the secondary filter 142.

[0054] In actual work, the first peristaltic pump 161 can be arranged in the backstage area 114, and the second peristaltic pump 162 can be arranged in the pollution area 113. The first peristaltic pump 161 and the second peristaltic pump 162 are electrically connected with the display controller 191. The dilution bottle 150, the first peristaltic pump 161, the sample jar 131, the primary filter 141, the second peristaltic pump 162, the secondary filter 142 and the bacterial liquid collecting bottle 182 are sequentially connected through pipelines.

[0055] The application also includes the odor purification bottle 171 and the negative pressure pump 172. One end of the negative pressure pump 172 is sequentially communicated with the odor purification bottle 171 and the crushing assembly 130, and the other end is sequentially communicated with the buffer 173 and the filter 174. The above-mentioned odor purification bottle 171 and the negative pressure pump 172 can remove the odor in the filtering and impurity removing box 108, so that the application is cleaner, more sanitary and more convenient to use.

[0056] In actual operation, the negative pressure pump 172 can be a negative pressure vacuum pump. One end of the negative pressure pump 172 is connected to the odor purification bottle 171 and the sample container 131 in sequence through pipelines, and the other end is connected to the buffer 173, the filter 174 and the bacterial liquid collection bottle 182 in sequence through pipelines.

[0057] Preferred, such as Figure 6 As shown, the buffer 173 includes a first buffer bottle 1731 and a second buffer bottle 1732 arranged sequentially along the gas flow direction. A first control valve 175 is provided between the odor purification bottle 171 and the sample container 131, and a second control valve 176 is provided between the filter 174 and the bacterial liquid collection bottle 182. In actual operation, both the first buffer bottle 1731 and the second buffer bottle 1732 can be pneumatic diaphragm pump buffer bottles, and both the first control valve 175 and the second control valve 176 can be pneumatic control valves.

[0058] A sample collection rack 181 is also set up in the contaminated area 113. Multiple bacterial liquid collection bottles 182 are installed on the sample collection rack 181. The bacterial liquid collection bottles 182 are used to collect the mixed liquid after being treated by the filter component 140.

[0059] The structure of bacterial culture collection bottle 182 is as follows: Figure 2 As shown, four bacterial liquid collection bottles 182 can be provided. Each bacterial liquid collection bottle 182 includes a bottle body and a bottle cap. The bottle body of the bacterial liquid collection bottle is provided with graduations, and the bottle cap of the bacterial liquid collection bottle is provided with a liquid inlet and a liquid outlet. The bottle body and the bottle cap of the bacterial liquid collection bottle 182 are connected by threads.

[0060] In actual work, the sample collection rack 181 and bacterial liquid collection bottle 182 mentioned above are not essential structures of this application. People can collect microbial mixtures using large-volume liquid collection bottles (such as Erlenmeyer flasks).

[0061] Preferably, the multiple bacterial culture collection bottles 182 are arranged in series, that is, the multiple bacterial culture collection bottles 182 are connected sequentially to the discharge end of the secondary filter 142. After the previous bacterial culture collection bottle 182 is filled with the microbial mixture (hereinafter referred to as bacterial culture), the next bacterial culture collection bottle 182 continues to be filled with the microbial mixture until the bacterial culture collection bottle 182 has completed the collection. Furthermore, a liquid level sensor can also be installed on the sample collection rack 181 to make it easier to collect the microbial mixture.

[0062] The clean area 112 is further provided with a display controller 191, and the sample crushing assembly 130, the filtering assembly 140 and the negative pressure assembly 160 are electrically connected with the display controller 191. The display controller 191 can be used to control the working state of the sample crushing assembly 130, the filtering assembly 140 and the negative pressure assembly 160, and the real-time working parameters of the sample crushing assembly 130, the filtering assembly 140 and the negative pressure assembly 160 can be directly displayed on the display controller 191, so that the working personnel can adjust the working parameters of the sample crushing assembly 130, the filtering assembly 140 and the negative pressure assembly 160 in real time according to the state of the fecal sample, thereby controlling the working state of the sample crushing assembly 130, the filtering assembly 140 and the negative pressure assembly 160 in real time.

[0063] The filtering and impurity removing box 108 is provided with a nitrogen tank 192 for placing nitrogen and a consumable tank 193 for placing consumables at the lower end, and the nitrogen tank 192 is located between the filtering and impurity removing box 108 and the consumable tank 193. Preferably, the filtering and impurity removing box 108 is provided with a nitrogen switch valve connected with the nitrogen cylinder and used to control the oxygen concentration in the filtering and impurity removing box 108.

[0064] Under the action of the nitrogen cylinder, the filtering and impurity removing box 108 can be in a low-oxygen state, thereby improving the survival rate of anaerobic bacteria in the feces; the filtering and impurity removing box 108 can be further provided with an oxygen sensor and the like, the oxygen sensor can be used to detect the oxygen concentration in the filtering and impurity removing box 108, and the display controller can control the opening or closing of the nitrogen switch valve according to the oxygen concentration fed back by the oxygen sensor, thereby controlling the oxygen concentration in the filtering and impurity removing box 108.

[0065] As shown in FIG. 1, Figure 6 the working steps of the present application are as follows:

[0066] S1, the water source (such as physiological saline) in the dilution bottle 150 is first introduced into the sample tank 131 under the action of the first peristaltic pump 161, at this time, the sample tank 131 contains a mixture of the water source (such as physiological saline) and the fecal sample, which is referred to as a first mixture;

[0067] S2, the sample crushing motor 132 below the sample tank 131 performs centrifugal crushing on the first mixture to obtain a mixture of the fecal sample after centrifugal crushing, which is referred to as a second mixture;

[0068] S3, the second mixture is sequentially treated by the primary filter 141, the second peristaltic pump 162 and the secondary filter 142 under the action of the second peristaltic pump 162 to form an intestinal microbial mixture (also referred to as a bacterial solution), and then is introduced into the bacterial solution collection bottle 182 (i.e., the bacterial solution collection bottle 182).

[0069] At this time, the bacteria liquid collecting bottle 182 mainly contains odor, intestinal microorganisms and aqueous solution, the intestinal microorganisms include various microorganisms such as beneficial bacteria, harmful bacteria, fungi and viruses; at this time, preferably, a part of the odor in the second mixture directly enters the odor purification bottle 171, and finally the exhaust gas is discharged from the odor purification bottle 171;

[0070] S4, under the action of the negative pressure pump 172, the bacteria liquid collecting bottle 182 forms a negative pressure, and the odor in the bacteria liquid collecting bottle 182 enters the odor purification bottle 171 in turn under the action of the negative pressure, passes through the gas filter 174, the first buffer bottle 1731, the second buffer bottle 1732 and the vacuum pump, and finally the exhaust gas is discharged from the odor purification bottle 171;

[0071] The two buffer bottles are pneumatic diaphragm buffer bottles, and the two pneumatic diaphragm buffer bottles can realize negative pressure buffering function, so that the pressure is balanced, and a negative pressure gauge can also be arranged.

[0072] Other parts not described in detail are prior art. Although the above embodiment has made a detailed description of the utility model, it is only a part of the embodiment of the utility model, not all embodiments, and people can also obtain other embodiments under the premise of no creativity according to the embodiment, and these embodiments all belong to the protection range of the utility model.

Claims

1. An apparatus for separating and collecting intestinal microorganisms, characterized by: The filter box (108), the first partition (121) and the second partition (122); The first partition (121) divides the filter box (108) into an operation area (111) and a backstage area (114), and the second partition (122) divides the operation area (111) into a clean area (112) and a contaminated area (113); The contaminated area (113) is provided with a crushing assembly (130) for crushing the fecal sample and a filter assembly (140) for filtering the mixture after crushing. The clean area (112) is provided with a dilution bottle (150). The first partition (121) is provided with a negative pressure assembly (160) for sequentially conveying water in the dilution bottle (150) to the crushing assembly (130) and the filter assembly (140).

2. The enteric microbial isolation collection device of claim 1, wherein: The crushing assembly (130) includes a sample tank (131) provided with a crushing shaft with crushing blades and a crushing motor (132) with an output end connected to the crushing shaft to drive the crushing shaft to rotate.

3. The enteric microbial isolation collection device of claim 2, wherein: The contaminated area (113) is provided with a first support plate (123) and a third support plate (125) for placing the sample tank (131), the crushing motor (132) is installed below the first support plate (123), the third support plate (125) is installed on the first support plate (123), and the third support plate (125) is provided with a crushing coupling (133), and the output end of the crushing motor (132) is connected to the crushing shaft through the crushing coupling (133).

4. The device of claim 3, wherein: The filter assembly (140) includes a primary filter (141) for primary filtering of the mixture after crushing and a secondary filter (142) for secondary filtering of the mixture after crushing, and the dilution bottle (150), the sample tank (131), the primary filter (141) and the secondary filter (142) are sequentially connected by pipelines.

5. The device of claim 4, wherein: The contaminated area (113) is provided with a second support plate (124) for placing the primary filter (141) and the secondary filter (142), the first support plate (123) is located below the second support plate (124), and the first support plate (123) and the second support plate (124) are connected by a fourth support plate (126).

6. The device of claim 5, wherein: The negative pressure assembly (160) includes a first peristaltic pump (161) installed between the dilution bottle (150) and the sample tank (131) and a second peristaltic pump (162) installed between the primary filter (141) and the secondary filter (142).

7. The enteric microbial isolation collection device of any one of claims 1 to 6, wherein: It also includes an odor purification bottle (171) and a negative pressure pump (172), one end of the negative pressure pump (172) is in communication with the odor purification bottle (171) and the crushing assembly (130) in sequence, and the other end is in communication with a buffer (173) and a filter (174) in sequence.

8. The enteric microbial isolation collection device of claim 7, wherein: The buffer (173) includes a first buffer bottle (1731) and a second buffer bottle (1732) arranged in sequence along the flow direction of the gas.

9. The enteric microbial isolation collection device of claim 8, wherein: A sample collection rack (181) is arranged in the pollution area (113), and a plurality of bacterial liquid collection bottles (182) are mounted on the sample collection rack (181) and used for collecting mixed liquid treated by the filtering assembly (140).

10. The enteric microbial isolation collection device of claim 9, wherein: The clean area (112) is further provided with a display controller (191), and the crushing assembly (130), the filtering assembly (140) and the negative pressure assembly (160) are electrically connected with the display controller (191).

11. The enteric microbial isolation collection device of claim 10, wherein: The filtering and impurity removing box (108) is provided with a nitrogen cabinet (192) for placing nitrogen and a consumable cabinet (193) for placing consumables at a lower end, and the nitrogen cabinet (192) is located between the filtering and impurity removing box (108) and the consumable cabinet (193).

12. The enteric microbial isolation collection device of claim 11, wherein: The filtering and impurity removing box (108) is further provided with one or more of a printer (101), a fan (102), a buzzer (103), a spotlight (104) and an illuminating lamp (105).

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