Micro-droplet type digital PCR (Polymerase Chain Reaction) detection device

Abstract

The utility model discloses a droplet type digital PCR (Polymerase Chain Reaction) detection device and relates to the technical field of nucleic acid detection. The microdroplet type digital PCR detection device comprises a rack, a first driving mechanism, a mounting rack, a liquid dropping mechanism, a bearing table, a temperature control mechanism and a shooting mechanism. The first driving mechanism is installed on the rack and connected with the installation frame, the liquid dropping mechanism and the shooting mechanism are both installed on the installation frame, the bearing table is connected with the rack, the temperature control mechanism is installed on the bearing table and used for bearing a reaction disc, the liquid dropping mechanism is used for injecting liquid drops to be detected into the reaction disc, and the temperature control mechanism is further used for controlling the temperature of the reaction disc. The shooting mechanism is used for carrying out fluorescence shooting on the to-be-detected liquid drops after the heating and cooling circulation of the to-be-detected liquid drops is completed. The droplet type digital PCR detection device provided by the utility model can realize automatic liquid dropping, thermal circulation and fluorescence shooting, is high in mechanization degree, saves time and labor, and improves the detection efficiency and the detection accuracy.

Description

TECHNICAL FIELD

[0001] The utility model relates to nucleic acid detection technical field, specifically, relate to a kind of microdrop digital PCR detection device. BACKGROUND

[0002] Microdrop digital PCR is an absolute quantitative PCR technique, which mainly adopts microdroplet method, disperses the solution containing target nucleic acid into a large number of water-in-oil droplets, forms tens of thousands of independent micro-reaction systems with equal volume, and the initial target nucleic acid template number of each droplet is 0, 1 or more. After PCR cycle, the droplet with one target nucleic acid will give a fluorescence signal, and the droplet without target nucleic acid will not have a fluorescence signal. According to the relative proportion of the two kinds of droplets and the mathematical correction of Poisson distribution, the target nucleic acid concentration of the original solution can be calculated.

[0003] Microdrop digital PCR needs to be cycled (using temperature control mechanism to cycle the droplet up and down) first, and then fluorescence shooting (using shooting mechanism to shoot and calculate the droplet). The current microdrop digital PCR detection device is to drop a certain amount of droplet into the reaction disc first, and then place the reaction disc on the heat conduction surface of the temperature control mechanism. Then the temperature control mechanism regulates the temperature of the droplet through the reaction disc, and then the shooting mechanism shoots the droplet after the temperature cycle. However, this series of operations is carried out manually, which is time-consuming and laborious, and the labor cost and time cost are high, the detection efficiency is low, and the detection accuracy is low.

[0004] Therefore, it is particularly important to design and manufacture a microdrop digital PCR detection device with high detection efficiency and high detection accuracy, especially in nucleic acid detection. UTILITY MODEL CONTENT

[0005] The utility model aims at providing a kind of microdrop digital PCR detection device, can realize automatic liquid drop, heat cycle and fluorescence shooting, degree of mechanization is high, save time and effort, improve detection efficiency, improve detection accuracy.

[0006] The utility model is realized by using the following technical solutions.

[0007] The application discloses a micro-droplet digital PCR detection device which comprises a rack, a first driving mechanism, a mounting rack, a droplet mechanism, a bearing table, a temperature control mechanism and a shooting mechanism.

[0008] Optionally, the mounting rack comprises a connecting plate and a mounting plate which are connected with each other, and the mounting plate is arranged perpendicularly to the connecting plate; the connecting plate is connected with the first driving mechanism; and the droplet mechanism and the shooting mechanism are arranged oppositely on two sides of the mounting plate and are connected with the mounting plate.

[0009] Optionally, the first driving mechanism comprises a first driving assembly, a translation rack and a second driving assembly; the first driving assembly is arranged on the rack and is connected with the translation rack; the first driving assembly is used for driving the translation rack to move in a first direction; the second driving assembly is arranged on the translation rack and is connected with the mounting rack; the second driving assembly is used for driving the mounting rack to move up and down in a second direction; and the first direction is perpendicular to the second direction.

[0010] Optionally, the micro-droplet digital PCR detection device further comprises a second driving mechanism; the second driving mechanism is arranged on the rack and is connected with the bearing table; and the second driving mechanism is used for driving the bearing table to move in a third direction; the first direction, the second direction and the third direction are perpendicular to each other.

[0011] Optionally, the micro-droplet digital PCR detection device further comprises a shell; the shell is arranged on the rack, the first driving mechanism, the mounting rack, the droplet mechanism, the bearing table, the temperature control mechanism and the shooting mechanism and is connected with the rack; and the shell is provided with a gap; and the gap is used for allowing the bearing table to extend out of the shell in the third direction.

[0012] Optionally, the shooting mechanism comprises a light-emitting assembly and a shooting assembly; the light-emitting assembly and the shooting assembly are arranged on the mounting rack; the light-emitting assembly is used for emitting excitation light towards the reaction disc; and the shooting assembly is used for receiving emission light to perform microscopic shooting on the droplet to be detected in the reaction disc.

[0013] Optionally, the light-emitting assembly comprises a heat sink, a light source, an excitation light wavelength selector and an excitation light beam adjuster; the light source is connected with the excitation light wavelength selector and the excitation light beam adjuster; and the heat sink is connected to one side of the light source which is away from the excitation light wavelength selector.

[0014] And / or, the shooting assembly comprises a shooting camera, a lens and an emitted light wavelength selector, the shooting camera is connected with the emitted light wavelength selector through the lens.

[0015] Optionally, the droplet mechanism comprises a third driving assembly and a syringe, the syringe comprises a needle tube and a push rod, the push rod is slidably arranged in the needle tube, the needle tube is connected with the mounting frame, the third driving assembly is mounted on the mounting frame and connected with the push rod, and the third driving assembly is used to drive the push rod to slide relative to the needle tube.

[0016] Optionally, the temperature control mechanism comprises a base, a fourth driving assembly, a temperature control module and a pressing plate, the base is connected with the bearing table, the fourth driving assembly and the temperature control module are both mounted on the base, the fourth driving assembly is in transmission connection with the pressing plate, and the fourth driving assembly is used to drive the pressing plate to rotate so as to press and hold the reaction disc on the temperature control module.

[0017] Optionally, the micro-droplet digital PCR detection device further comprises an accessory disc and a sample disc, the accessory disc and the sample disc are both mounted on the bearing table, the accessory disc is used to contain a needle and an outer tube which are detachably connected with the droplet mechanism, and the sample disc is used to contain a sample solution storage tube.

[0018] The micro-droplet digital PCR detection device provided by the utility model has the following beneficial effects:

[0019] The micro-droplet digital PCR detection device provided by the utility model, the first driving mechanism is mounted on the rack and connected with the mounting frame, the droplet mechanism and the shooting mechanism are both mounted on the mounting frame, the first driving mechanism is used to drive the droplet mechanism and the shooting mechanism to move synchronously through the mounting frame, the bearing table is connected with the rack, the temperature control mechanism is mounted on the bearing table, the temperature control mechanism is used to bear the reaction disc, the droplet mechanism is used to drop the liquid to be tested into the reaction disc, the temperature control mechanism is further used to control the temperature of the reaction disc, so that the liquid to be tested is subjected to temperature rising and falling circulation, and the shooting mechanism is used to shoot the fluorescence of the liquid to be tested after the temperature rising and falling circulation is completed. Compared with the prior art, the micro-droplet digital PCR detection device provided by the utility model can realize automatic droplet, thermal cycle and fluorescence shooting, has high mechanization degree, saves time and effort, improves detection efficiency and detection precision. BRIEF DESCRIPTION OF DRAWINGS

[0020] In order to more clearly illustrate the technical scheme of the embodiments of the utility model, the following will briefly introduce the drawings needed to be used in the embodiments, and it should be understood that the following drawings only show some embodiments of the utility model, and should not be regarded as a limitation to the scope, and for those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.

[0021] Figure 1 A structure schematic view of the micro-droplet digital PCR detection device provided by the embodiment of the present application when the bearing table extends out of the shell;

[0022] Figure 2 A structure schematic view of the micro-droplet digital PCR detection device provided by the embodiment of the present application from one perspective;

[0023] Figure 3 A structure schematic view of the micro-droplet digital PCR detection device provided by the embodiment of the present application from another perspective;

[0024] Figure 4 A structure schematic view of the shooting mechanism in the micro-droplet digital PCR detection device provided by the embodiment of the present application;

[0025] Figure 5 A structure schematic view of the droplet mechanism in the micro-droplet digital PCR detection device provided by the embodiment of the present application;

[0026] Figure 6 A structure schematic view of the micro-droplet digital PCR detection device provided by the embodiment of the present application; Figure 5 A sectional view of the syringe in the micro-droplet digital PCR detection device provided by the embodiment of the present application;

[0027] Figure 7 A structure schematic view of the accessory disc and the sample disc in the micro-droplet digital PCR detection device provided by the embodiment of the present application;

[0028] Figure 8 A structure schematic view of the first and second discharge grooves on the bearing table in the micro-droplet digital PCR detection device provided by the embodiment of the present application;

[0029] Figure 9 A structure schematic view of the temperature control mechanism in the micro-droplet digital PCR detection device provided by the embodiment of the present application.

[0030] Icon: 100-droplet digital PCR detection device; 110-rack; 120-first driving mechanism; 121-first driving assembly; 122-translation frame; 123-second driving assembly; 130-mounting frame; 131-connection plate; 132-mounting plate; 140-droplet mechanism; 141-third driving assembly; 142-syringe; 1421-needle tube; 1422-push rod; 1423-needle; 1424-outer tube; 150-carrier table; 151-first discharge groove; 152-second discharge groove; 160-temperature control mechanism; 161-base; 162-fourth driving assembly; 163-temperature control module; 164-pressing plate; 170-shooting mechanism; 171-emitting assembly; 1711-radiator; 1712-light source; 1713-excitation light wavelength selector; 1714-excitation light beam adjuster; 172-shooting assembly; 1721-shooting camera; 1722-lens; 1723-emission light wavelength selector; 180-second driving mechanism; 190-outer shell; 191-let place; 200-fittings disc; 210-sample disc; 211-sample solution storage tube; 300-reaction disc. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme of the embodiments of the present application will be described clearly and completely below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments of the present application. The components of the embodiments of the present application described and shown in the drawings herein can be arranged and designed in various different configurations.

[0032] Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed present application, but only represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application.

[0033] It should be noted that: similar reference numerals and letters represent similar items in the following drawings, therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0034] In the description of the utility model, it needs to be explained that the terms "inner", "outer", "upper", "lower", "horizontal" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship commonly used when the utility model product is used, which is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the device or element indicated must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the utility model. In addition, the terms "first", "second", "third" and the like are only used for differentiation in description and cannot be understood as indicating or implying relative importance.

[0035] In the description of the utility model, it also needs to be explained that, unless otherwise explicitly specified and limited, the terms "set", "connected", "mounted", "connected" should be broadly understood, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the communication between two elements inside. For ordinary skilled persons in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.

[0036] Some embodiments of the utility model will be described in detail below in combination with the drawings. The features in the following examples can be combined with each other without conflict.

[0037] Please refer to Figures 1 to 3 The utility model embodiment provides a kind of micro droplet digital PCR detection device 100 for carrying out nucleic acid detection. It can realize automatic droplet, thermal cycle and fluorescence shooting, high degree of mechanization, save time and effort, improve detection efficiency, improve detection accuracy.

[0038] The micro-droplet digital PCR detection device 100 comprises a rack 110, a first driving mechanism 120, a mounting rack 130, a droplet mechanism 140, a bearing table 150, a temperature control mechanism 160 and a shooting mechanism 170. The first driving mechanism 120 is mounted on the rack 110 and connected with the mounting rack 130. The droplet mechanism 140 and the shooting mechanism 170 are both mounted on the mounting rack 130. The first driving mechanism 120 is used to drive the droplet mechanism 140 and the shooting mechanism 170 to move synchronously through the mounting rack 130, so as to realize the displacement function of the droplet mechanism 140 and the shooting mechanism 170, and facilitate the movement of the droplet mechanism 140 and the shooting mechanism 170 to different positions. The bearing table 150 is connected with the rack 110. The temperature control mechanism 160 is mounted on the bearing table 150. The temperature control mechanism 160 is used to carry the reaction disc 300. The droplet mechanism 140 is used to inject the to-be-tested liquid droplets into the reaction disc 300. The temperature control mechanism 160 is also used to control the temperature of the reaction disc 300, so as to make the to-be-tested liquid droplets undergo temperature cycling. The shooting mechanism 170 is used to take fluorescence photos of the to-be-tested liquid droplets after the temperature cycling is completed.

[0039] Specifically, the droplet mechanism 140 is used to make a large number of water-in-oil droplets (to-be-tested liquid droplets) from the original solution in one sample, and inject these to-be-tested liquid droplets into the reaction disc 300 placed on the temperature control mechanism 160. The temperature control mechanism 160 is used to make the to-be-tested liquid droplets undergo temperature cycling through the reaction disc 300. The number of target nucleic acids in each to-be-tested liquid droplet is doubled after each temperature cycling. The process is repeated to greatly increase the number of trace target nucleic acids. The shooting device is used to take photos and calculate all the to-be-tested liquid droplets after the temperature cycling is completed. The to-be-tested liquid droplets with target nucleic acids will give fluorescence signals, and the to-be-tested liquid droplets without target nucleic acids will not give fluorescence signals. According to the relative proportion of the number of the two kinds of to-be-tested liquid droplets and the Poisson distribution mathematical correction, the target nucleic acid concentration of the original solution in the corresponding sample can be calculated.

[0040] Under the action of the first driving mechanism 120, the droplet mechanism 140 can suck the original solution in a sample at one position, generate to-be-tested liquid droplets at another position and inject them into the reaction disc 300, realizing automatic droplet operation. Then the reaction disc 300 can make the to-be-tested liquid droplets undergo temperature cycling, realizing automatic thermal cycling operation. Then the shooting mechanism 170 can move to a suitable position under the action of the first driving mechanism 120 to take photos of the to-be-tested liquid droplets in the reaction disc 300, realizing automatic fluorescence shooting operation. In this way, the micro-droplet digital PCR detection device 100 can realize automatic droplet, thermal cycling and fluorescence shooting, has high mechanization degree, saves time and effort, improves detection efficiency and detection accuracy.

[0041] The mounting frame 130 comprises a connecting plate 131 and a mounting plate 132 connected with each other. The mounting plate 132 is arranged perpendicularly to the connecting plate 131. The connecting plate 131 is connected with the first driving mechanism 120. The droplet mechanism 140 and the shooting mechanism 170 are arranged oppositely on two sides of the mounting plate 132 and are connected with the mounting plate 132. The mounting plate 132 is used to separate the droplet mechanism 140 and the shooting mechanism 170 to prevent them from interfering with each other and affecting each other and facilitate independent disassembly and maintenance of the droplet mechanism 140 and the shooting mechanism 170. Specifically, the first driving mechanism 120 can drive the mounting plate 132 to move through the connecting plate 131, thereby synchronously driving the droplet mechanism 140 and the shooting mechanism 170 to move. The driving function of two components (the droplet mechanism 140 and the shooting mechanism 170) can be realized through the same driving structure (the first driving mechanism 120), which greatly reduces the volume of the droplet digital PCR detection device 100 and saves the equipment cost.

[0042] The first driving mechanism 120 comprises a first driving assembly 121, a translation frame 122 and a second driving assembly 123. The first driving assembly 121 is mounted on the rack 110 and is connected with the translation frame 122. The first driving assembly 121 is used to drive the translation frame 122 to move in a first direction. The second driving assembly 123 is mounted on the translation frame 122 and is connected with the mounting frame 130. The second driving assembly 123 is used to drive the mounting frame 130 to move up and down in a second direction. Specifically, the first driving assembly 121 can synchronously drive the droplet mechanism 140 and the shooting mechanism 170 to move in the first direction through the translation frame 122, the second driving assembly 123 and the mounting frame 130 in sequence. The second driving assembly 123 can synchronously drive the droplet mechanism 140 and the shooting mechanism 170 to move up and down in the second direction through the mounting frame 130. The first direction is perpendicular to the second direction, so that the droplet mechanism 140 and the shooting mechanism 170 can move to any position on the plane where the first direction and the second direction are located, thereby improving the movement precision.

[0043] In the embodiment, the first direction is a horizontal direction, the second direction is a vertical direction, and the plane where the first direction and the second direction are located is a vertical plane. The first driving mechanism 120 can drive the droplet mechanism 140 and the shooting mechanism 170 to move to any position on the vertical plane, thereby facilitating the droplet and fluorescence shooting operation.

[0044] Preferably, the micro-droplet digital PCR detection device 100 further comprises a second driving mechanism 180. The second driving mechanism 180 is mounted on the rack 110 and connected with the bearing table 150, and the second driving mechanism 180 is used to drive the bearing table 150 to move in a third direction. Specifically, the second driving mechanism 180 can drive the temperature control mechanism 160 and the reaction disc 300 to move in the third direction through the bearing table 150 synchronously, and the first direction, the second direction and the third direction are perpendicular to each other, so that the droplet mechanism 140 and the shooting mechanism 170 can move to any position on the bearing table 150, and the droplet position accuracy and the shooting position accuracy are ensured.

[0045] Further, the number of the temperature control mechanism 160 and the reaction disc 300 is multiple, multiple temperature control mechanisms 160 are arranged side by side on the bearing table 150, each reaction disc 300 is placed on one temperature control mechanism 160, and the first driving mechanism 120 and the second driving mechanism 180 jointly act to enable the syringe 142 to inject the droplets of different samples to be tested into different reaction discs 300, so as to facilitate the nucleic acid detection of multiple samples at the same time and improve the detection efficiency.

[0046] In the embodiment, the number of the temperature control mechanism 160 and the reaction disc 300 is four, but is not limited thereto. In other embodiments, the number of the temperature control mechanism 160 and the reaction disc 300 can be two or eight, and the number of the temperature control mechanism 160 and the reaction disc 300 is not specifically limited.

[0047] Preferably, the micro-droplet digital PCR detection device 100 further comprises a shell 190. The shell 190 is simultaneously arranged outside the rack 110, the first driving mechanism 120, the mounting rack 130, the droplet mechanism 140, the bearing table 150, the temperature control mechanism 160 and the shooting mechanism 170, and connected with the rack 110. The rack 110 is used to fix the shell 190, the shell 190 is used to shield the external dust, the shell 190 is also used to shield the external light (which is conducive to fluorescence shooting), and the shell 190 is also used to improve the aesthetic appearance. Specifically, the shell 190 is provided with a gap 191, and the gap 191 is used to allow the bearing table 150 to extend out of the shell 190 in the third direction. The bearing table 150 can drive the temperature control mechanism 160 and the reaction disc 300 to extend out in the third direction synchronously under the action of the second driving mechanism 180, so as to facilitate the replacement of the reaction disc 300 on the temperature control mechanism 160, thereby facilitating the next nucleic acid detection.

[0048] The shooting mechanism 170 comprises a light-emitting assembly 171 and a shooting assembly 172. The light-emitting assembly 171 and the shooting assembly 172 are both mounted on the mounting frame 130. The light-emitting assembly 171 is used for emitting excitation light towards the reaction disc 300, and the shooting assembly 172 is used for receiving emission light to perform microscopic shooting on the to-be-tested liquid drops in the reaction disc 300, so as to realize the fluorescence shooting function. Specifically, during the fluorescence shooting process, the light-emitting assembly 171 emits excitation light towards the to-be-tested liquid drops in the reaction disc 300 to excite the fluorescent molecules in the to-be-tested liquid drops to make them transition from a ground state to an excited state. The excitation light usually has a shorter wavelength and higher energy, which can promote the energy level transition of the electrons in the fluorescent molecules after absorbing energy. After being excited, the fluorescent molecules return to the ground state from the excited state, and in this process, emission light is released for the shooting mechanism 170 to receive. Since the fluorescent molecules have energy loss during the process of returning to the ground state from the excited state, the wavelength of the emission light is longer than that of the excitation light, and the energy is lower.

[0049] Please refer to Figure 4 Preferably, the light-emitting assembly 171 comprises a heat sink 1711, a light source 1712, an excitation light wavelength selector 1713 and an excitation light beam adjuster 1714. The light source 1712 is connected with the excitation light beam adjuster 1714 through the excitation light wavelength selector 1713. The heat sink 1711 is connected to the side of the light source 1712 away from the excitation light wavelength selector 1713. The light source 1712 is used for emitting LED light (excitation light). The excitation light wavelength selector 1713 is used for selecting the wavelength of the emitted excitation light. The heat sink 1711 is used for heat dissipation and cooling of the light source 1712 to prolong the service life of the light source 1712.

[0050] Preferably, the shooting assembly 172 comprises a shooting camera 1721, a lens 1722 and an emission light wavelength selector 1723. The shooting camera 1721 is connected with the emission light wavelength selector 1723 through the lens 1722. The emission light wavelength selector 1723 is used for selecting the wavelength of the received emission light. The shooting camera 1721 is used for performing microscopic shooting on the to-be-tested liquid drops in the reaction disc 300 through the lens 1722.

[0051] Please refer to Figures 5 to 7The drop mechanism 140 comprises a third driving assembly 141 and a syringe 142. The syringe 142 comprises a needle tube 1421 and a push rod 1422, the push rod 1422 is slidably arranged in the needle tube 1421, the push rod 1422 can slide relative to the needle tube 1421 to form negative pressure or positive pressure in the needle tube 1421, thereby realizing the liquid suction function and the drop function, the needle tube 1421 can guide and limit the push rod 1422. Specifically, the needle tube 1421 is connected with the mounting frame 130, the mounting frame 130 can fix the needle tube 1421, the third driving assembly 141 is installed on the mounting frame 130 and connected with the push rod 1422, the third driving assembly 141 is used to drive the push rod 1422 to slide relative to the needle tube 1421, so as to realize the automatic action of the push rod 1422, without manual operation, improve the drop efficiency, improve the drop amount precision, save the labor cost and time cost.

[0052] Further, the third driving assembly 141 is connected with the push rod 1422 of the syringe 142, the third driving assembly 141 is used to drive the syringe 142 to suck the original solution in the sample, and the third driving assembly 141 is also used to drive the syringe 142 to drop the original solution outward, in this process, the oil pump discharges the oil and wraps the oil droplets outside the original solution droplets, so as to form water-in-oil droplets (to-be-measured droplets), thereby realizing the function of injecting the to-be-measured droplets into the reaction disc 300.

[0053] In this embodiment, the needle tube 1421 and the push rod 1422 are arranged in the vertical direction, the inlet and outlet of the needle tube 1421 are located at the bottom thereof, the third driving assembly 141 is connected to the top of the push rod 1422, the third driving assembly 141 can drive the push rod 1422 to slide upward relative to the needle tube 1421 to realize the liquid suction function through the inlet and outlet, and the third driving assembly 141 can also drive the push rod 1422 to slide downward relative to the needle tube 1421 to realize the drop function through the inlet and outlet.

[0054] It should be noted that the syringe 142 further comprises a needle 1423 and an outer tube 1424, both of which are used in cooperation with the needle tube 1421 and are consumables. Specifically, during the dripping process, the needle 1423 is first installed on the needle tube 1421 of the syringe 142; then the needle 1423 is inserted into the sample solution storage tube 211 to suck the original solution in the sample solution storage tube 211 into the needle 1423 (the amount of suction is very small, about 20 μL, so the original solution will not enter the needle tube 1421); then the outer tube 1424 is installed on the needle tube 1421 so that the outer tube 1424 is spacedly sleeved outside the needle 1423; then the original solution is dripped out, and the oil pump is controlled to synchronously discharge oil from the gap between the outer tube 1424 and the needle 1423 to wrap the oil droplets on the outside of the original solution droplets to form water-in-oil droplets (the droplets of the sample to be tested) and drop onto the reaction disc 300. In this way, only the needle 1423 and the outer tube 1424 contact the original solution of the sample during each dripping process, and to avoid cross contamination of multiple samples, the used needle 1423 and the outer tube 1424 need to be removed and replaced with new ones after each dripping process to facilitate the next dripping process.

[0055] Preferably, the micro-droplet digital PCR detection device 100 further comprises an accessory disc 200 and a sample disc 210. The accessory disc 200 and the sample disc 210 are both installed on the bearing table 150, the accessory disc 200 is used to store the needle 1423 and the outer tube 1424 which are detachably connected with the dripping mechanism 140, and the sample disc 210 is used to store the sample solution storage tube 211. Specifically, the assembly openings of the needle 1423 and the outer tube 1424 are both arranged upward, and during the installation of the needle 1423 or the outer tube 1424, the first driving mechanism 120 and the second driving mechanism 180 jointly act to first align the bottom end of the needle tube 1421 with the assembly opening of the needle 1423 or the outer tube 1424, and then press the needle tube 1421 downward to make the needle tube 1421 and the needle 1423 or the outer tube 1424 be in clamping cooperation. In addition, the tube opening of the sample solution storage tube 211 is arranged upward, and during the suction of the original solution, the first driving mechanism 120 and the second driving mechanism 180 first jointly act to make the needle 1423 extend into the sample solution storage tube 211; then the third driving assembly 141 drives the push rod 1422 to slide upward to suck the original solution in the sample solution storage tube 211 into the needle 1423.

[0056] Please refer to Figure 8Further, the bearing table 150 is provided with a first discharging groove 151 and a second discharging groove 152, the area of the first discharging groove 151 is greater than that of the second discharging groove 152, wherein the first discharging groove 151 is used for dismounting the outer tube 1424 from the needle tube 1421, and the second discharging groove 152 is used for dismounting the needle 1423 from the needle tube 1421. Specifically, after the completion of one drop operation, the needle 1423 and the outer tube 1424 need to be replaced to avoid cross contamination of multiple samples, at this time, the first driving mechanism 120 and the second driving mechanism 180 jointly act, first drive the needle tube 1421 to extend into the first discharging groove 151, so that the outer tube 1424 is located below the first discharging groove 151, then drive the needle tube 1421 to move upward to dismount the outer tube 1424, then drive the needle tube 1421 to extend into the second discharging groove 152, so that the needle 1423 is located below the second discharging groove 152, then drive the needle tube 1421 to move upward to dismount the needle 1423.

[0057] In the embodiment, the first driving assembly 121 drives the translation frame 122 to move by driving the lead screw to rotate and cooperating the lead screw with the nut, the driving modes of the second driving assembly 123, the third driving assembly 141 and the second driving mechanism 180 are the same as that of the first driving assembly 121, which will not be repeated here. However, it is not limited thereto, in other embodiments, the first driving assembly 121, the second driving assembly 123, the third driving assembly 141 and the second driving mechanism 180 can also be driven by air cylinder or hydraulic cylinder, and the driving modes of the first driving assembly 121, the second driving assembly 123, the third driving assembly 141 and the second driving mechanism 180 are not specifically limited.

[0058] Please refer to Figure 9 The temperature control mechanism 160 includes a base 161, a fourth driving assembly 162, a temperature control module 163 and a pressing plate 164. The base 161 is connected with the bearing table 150, and the bearing table 150 can support and fix the base 161. The fourth driving assembly 162 and the temperature control module 163 are both installed on the base 161, and the temperature control module 163 is used for carrying the reaction disc 300 and controlling the temperature of the reaction disc 300 to realize the temperature rising and falling cycle function of the measured liquid drop. Specifically, the fourth driving assembly 162 is in transmission connection with the pressing plate 164, and the fourth driving assembly 162 is used for driving the pressing plate 164 to rotate to press and hold the reaction disc 300 on the temperature control module 163, so as to press the reaction disc 300 and the temperature control module 163 tightly, ensure that the reaction disc 300 and the temperature control module 163 are closely and uniformly attached, thereby enhancing the heat conduction effect, improving the temperature rising and falling rate, and ensuring uniform temperature distribution.

[0059] In the embodiment, the fourth driving assembly 162 drives the pressing plate 164 to rotate in a manner of driving motor combined with belt transmission, but is not limited thereto, in other embodiments, the driving motor can be directly used to drive the pressing plate 164 to rotate, or the pneumatic motor or hydraulic motor can be directly or indirectly used to drive the pressing plate 164 to rotate, and the driving mode of the fourth driving assembly 162 is not specifically limited.

[0060] It should be noted that the plurality of temperature control mechanisms 160 are independently controlled, so that the pressing function and temperature control function of the plurality of temperature control mechanisms 160 do not interfere with each other, and the driving function of the first driving mechanism 120 and the second driving mechanism 180 also do not interfere with each other, which greatly improves the flexibility and detection efficiency of the micro-droplet digital PCR detection device 100. For example, when the droplet in the first reaction disc 300 is completed, the corresponding temperature control mechanism 160 immediately presses and performs temperature cycling, at this time, the first driving mechanism 120 and the second driving mechanism 180 can cooperate with each other to continue to inject the droplet to be tested into the second reaction disc 300, and so on. The reaction disc 300 that completes the temperature cycling can be preferentially photographed (the first driving mechanism 120 and the second driving mechanism 180 cooperate to drive the photographing mechanism 170 to move), and in this process, the reaction disc 300 that does not complete the temperature cycling does not need the first driving mechanism 120 and the second driving mechanism 180 to work. In addition, since each temperature control mechanism 160 presses and controls the temperature of one reaction disc 300, no matter how many temperature control mechanisms 160 are installed in the micro-droplet digital PCR detection device 100 (full or not full), there will be no problem of uneven pressing, thereby ensuring the temperature control effect and temperature uniformity.

[0061] The micro-droplet digital PCR detection device 100 provided by the embodiment of the utility model, first driving mechanism 120 is installed in frame 110, and is connected with mounting bracket 130, droplet mechanism 140 and photographing mechanism 170 are all installed in mounting bracket 130, first driving mechanism 120 is used for driving droplet mechanism 140 and photographing mechanism 170 to move synchronously through mounting bracket 130, bearing table 150 is connected with frame 110, temperature control mechanism 160 is installed in bearing table 150, temperature control mechanism 160 is used for bearing reaction disc 300, droplet mechanism 140 is used for injecting the droplet to be tested into reaction disc 300, temperature control mechanism 160 is also used for controlling the temperature of reaction disc 300 to make the droplet to be tested to carry out temperature cycling, photographing mechanism 170 is used for carrying out fluorescence photography to the droplet to be tested after temperature cycling. Compared with the prior art, the micro-droplet digital PCR detection device 100 provided by the utility model can realize automatic droplet, thermal cycling and fluorescence photography because of the adoption of the droplet mechanism 140 and the photographing mechanism 170 installed in the mounting bracket 130 and the temperature control mechanism 160 installed in the bearing table 150, so the degree of mechanization is high, time and labor are saved, the detection efficiency is improved, and the detection accuracy is improved.

[0062] The preferred embodiments of the present application have been described above with the preferred embodiments, but are not intended to limit the present application. For those skilled in the art, the present application can have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A droplet digital PCR detection device, characterized by, The micro-droplet digital PCR detection device comprises a rack, a first driving mechanism, a mounting rack, a droplet mechanism, a bearing table, a temperature control mechanism and a shooting mechanism, the first driving mechanism is installed on the rack and connected with the mounting rack, the droplet mechanism and the shooting mechanism are both installed on the mounting rack, the first driving mechanism is used to drive the droplet mechanism and the shooting mechanism to move synchronously through the mounting rack, the bearing table is connected with the rack, the temperature control mechanism is installed on the bearing table, the temperature control mechanism is used to carry a reaction disc, the droplet mechanism is used to drop a to-be-tested liquid into the reaction disc, the temperature control mechanism is also used to control the temperature of the reaction disc to make the to-be-tested liquid drop to circulate in the temperature, and the shooting mechanism is used to shoot the fluorescence of the to-be-tested liquid drop after the temperature circulation is completed.

2. The droplet digital PCR detection device according to claim 1, wherein, The mounting rack comprises a connecting plate and a mounting plate connected with each other, the mounting plate is arranged perpendicularly to the connecting plate, the connecting plate is connected with the first driving mechanism, and the droplet mechanism and the shooting mechanism are arranged oppositely on two sides of the mounting plate and connected with the mounting plate.

3. The droplet digital PCR detection device according to claim 1, wherein, The first driving mechanism comprises a first driving assembly, a translation frame and a second driving assembly, the first driving assembly is installed on the rack and connected with the translation frame, the first driving assembly is used to drive the translation frame to move in a first direction, the second driving assembly is installed on the translation frame and connected with the mounting rack, and the second driving assembly is used to drive the mounting rack to move up and down in a second direction, the first direction is perpendicular to the second direction.

4. The droplet digital PCR detection device according to claim 3, wherein, The micro-droplet digital PCR detection device further comprises a second driving mechanism, the second driving mechanism is installed on the rack and connected with the bearing table, the second driving mechanism is used to drive the bearing table to move in a third direction, and the first direction, the second direction and the third direction are perpendicular to each other.

5. The droplet digital PCR detection device according to claim 4, wherein, The micro-droplet digital PCR detection device further comprises a shell, the shell is arranged on the rack, the first driving mechanism, the mounting rack, the droplet mechanism, the bearing table, the temperature control mechanism and the shooting mechanism, and connected with the rack, the shell is provided with a gap, and the gap is used for the bearing table to extend out of the shell in the third direction.

6. The droplet digital PCR detection device of claim 1, wherein, The shooting mechanism comprises a light-emitting assembly and a shooting assembly, the light-emitting assembly and the shooting assembly are both installed on the mounting rack, the light-emitting assembly is used to emit excitation light to the reaction disc, and the shooting assembly is used to receive emission light to shoot the to-be-tested liquid drop in the reaction disc.

7. The droplet digital PCR detection device of claim 6, wherein, The light-emitting assembly comprises a heat sink, a light source, an excitation light wavelength selector and an excitation light beam adjuster, the light source is connected with the excitation light wavelength selector and the excitation light beam adjuster, and the heat sink is connected to one side of the light source away from the excitation light wavelength selector. And / or, the shooting assembly comprises a shooting camera, a lens and an emission light wavelength selector, the shooting camera is connected with the lens and the emission light wavelength selector.

8. The droplet digital PCR detection device of claim 1, wherein, The droplet mechanism comprises a third driving assembly and a syringe, the syringe comprises a needle tube and a push rod, the push rod is slidably arranged in the needle tube, the needle tube is connected with the mounting frame, the third driving assembly is mounted on the mounting frame and connected with the push rod, and the third driving assembly is used to drive the push rod to slide relative to the needle tube.

9. The droplet digital PCR detection device of claim 1, wherein, The temperature control mechanism comprises a base, a fourth driving assembly, a temperature control module and a pressing plate, the base is connected with the bearing table, the fourth driving assembly and the temperature control module are both mounted on the base, the fourth driving assembly is in transmission connection with the pressing plate, and the fourth driving assembly is used to drive the pressing plate to rotate so as to press and hold the reaction disc on the temperature control module.

10. The droplet digital PCR detection device of claim 1, wherein, The micro-droplet digital PCR detection device further comprises an accessory disc and a sample disc, the accessory disc and the sample disc are both mounted on the bearing table, the accessory disc is used to accommodate a needle and an outer tube which are detachably connected with the droplet mechanism, and the sample disc is used to accommodate a sample solution storage tube.

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