Volume-adjustable cell culture and biochemical reaction detection device

Abstract

The utility model discloses a volume-adjustable cell culture and biochemical reaction detection device, which comprises a micro-reaction container, a test chip and an overflow detection mechanism, a micro-reaction chamber for containing a liquid phase system is arranged in the micro-reaction container, the test chip is arranged in the micro-reaction chamber, and the overflow detection mechanism is arranged in the micro-reaction chamber. The micro-reaction container is provided with a telescopic structure, the telescopic structure can stretch out and draw back in the axial direction of the micro-reaction container under the action of external force, so that the volume of the micro-reaction cavity is changed, an overflow groove and an observation window are further formed in the top of the micro-reaction container, the overflow groove is communicated with the micro-reaction cavity, and the observation window is communicated with the overflow groove. The overflow detection mechanism is arranged in the overflow groove, and the overflow detection mechanism can generate changes which can be visually observed after being in contact with the liquid phase system. According to the volume-adjustable cell culture and biochemical reaction detection device provided by the utility model, the volume can be adjusted according to actual requirements, and the practicability is high.

Description

TECHNICAL FIELD

[0001] The utility model relates to a volume adjustable cell culture and biochemical reaction detection device especially belongs to biomedical engineering or biological technology field. BACKGROUND

[0002] Cell culture and biological reaction detection are indispensable links in modern biological research and biotechnology application. By simulating the growth environment of cells in vivo, cells can be cultured in vitro, which can deeply study the physiological characteristics, growth law and reaction to various stimuli of cells. At the same time, cell culture also provides an important experimental model for the fields of drug research and development, tissue engineering, etc. However, in the process of cell culture, the volume requirement of the culture container often changes temporarily (larger volume means that the corresponding experimental operation can be more efficient and flexible) in different experimental steps, especially dilution and washing.

[0003] Biological reaction detection is a key means to understand the mechanism of life activities and the development of diseases. With the continuous deepening of life science research, the demand for efficient and accurate detection of biological reactions is increasing. Traditional biological detection methods are often complex, time-consuming and require a large amount of samples and reagents. The emergence of biochip technology provides a new way to solve these problems. Biochip can integrate a large number of biological probes such as nucleic acids, proteins and antibodies in a very small area, and can detect multiple biomarkers at the same time. In biological reaction detection, biochip can quickly capture the interaction information between biological molecules with less sample, and realize real-time monitoring of the biological reaction process. For example, in disease diagnosis, biochip can quickly and accurately determine the type and state of disease by detecting specific biomarkers in patient samples. In the field of drug research and development, biochip can be used for screening drug targets and evaluating drug efficacy. However, in the process of using biochip to detect biological reactions, the optimization of detection performance (reaction rate is often related to the concentration of each reactant and the volume of liquid environment) and the bioactivity treatment (protein solidification) process of the chip also exist the case of temporary change of volume requirement of the culture container (larger volume means that the corresponding experimental operation can be more efficient and flexible).

[0004] Therefore, a new type of detection device is needed to adapt to the change of the volume of the detection device in the process of cell culture and biological reaction detection experimental operation. UTILITY MODEL CONTENT

[0005] The main purpose of the utility model is to provide a volume adjustable cell culture and biochemical reaction detection device, so as to overcome the defects in the prior art.

[0006] To achieve the aforementioned utility model purposes, the utility model employs the technical scheme including:

[0007] The utility model discloses a volume adjustable cell culture and biochemical reaction detection device, it includes micro -reaction container, test chip and full -spill detection mechanism, the micro -reaction container inside has the micro -reaction chamber for containing liquid phase system, the test chip sets up in the micro -reaction chamber, the micro -reaction container has telescopic structure, the telescopic structure can telescopic under the action of external force along the axial direction of the micro -reaction container, and make the volume of the micro -reaction chamber change, and, the top of micro -reaction container still is provided with overflow groove and observation window, the observation window with the overflow groove correspond, the overflow groove with the micro -reaction chamber is linked together, the full -spill detection mechanism sets up in the overflow groove and is located the observation area of observation window, the full -spill detection mechanism can change after contacting with the liquid phase system and can be visually observed.

[0008] Compared with the prior art, the utility model has the advantages of:

[0009] 1) the volume adjustable cell culture and biochemical reaction detection device provided by the utility model can conveniently adjust the capacity of the micro -reaction chamber according to actual needs through the adjusting knob, and is practical.

[0010] 2) the volume adjustable cell culture and biochemical reaction detection device provided by the utility model can remind the operator that the liquid in the micro -reaction container is full when the full -spill detection mechanism contacts the liquid and changes color when the liquid in the micro -reaction chamber exceeds the set volume during operation. BRIEF DESCRIPTION OF DRAWINGS

[0011] In order to more clearly illustrate the technical scheme in the embodiments of the present application or the prior art, the drawings needed in the embodiment or prior art description will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments described in the present application, and those skilled in the art can obtain other drawings according to these drawings without creating any creative labor.

[0012] Figure 1 It is the first view schematic drawing of the volume adjustable cell culture and biochemical reaction detection device provided in a typical embodiment of the utility model;

[0013] Figure 2 It is the second view schematic drawing of the volume adjustable cell culture and biochemical reaction detection device provided in a typical embodiment of the utility model;

[0014] Figure 3It is the third view schematic diagram of the volume-adjustable cell culture and biochemical reaction detection device provided in a typical embodiment case of the utility model.

[0015] Figure 4 It is the fourth view schematic diagram of the volume-adjustable cell culture and biochemical reaction detection device provided in a typical embodiment case of the utility model.

[0016] Figure 5 It is the internal structure schematic diagram of the volume-adjustable cell culture and biochemical reaction detection device provided in a typical embodiment case of the utility model.

[0017] Figure 6 It is the schematic diagram of the volume-adjustable cell culture and biochemical reaction detection device adjusted to a certain volume state provided in a typical embodiment case of the utility model.

[0018] The drawing mark: 1 micro reaction container, 101 container wall, 1011 corrugated structure, 102 base, 103 top cover, 104 sleeve part, 2 test chip, 3 waterproof compression sheet, 4 observation window, 5 liquid immersion indicating test paper, 6 adjusting knob. Specific implementation

[0019] In view of the deficiencies in the prior art, the present inventors have long-term research and a large number of practices, and have proposed the technical scheme of the utility model. The technical scheme, its implementation process and principles will be further explained and described as follows.

[0020] The utility model embodiment provides a kind of volume-adjustable cell culture and biochemical reaction detection device, comprising: micro reaction container, test chip and overflow detection mechanism, the micro reaction container inside has the micro reaction chamber for containing liquid phase system, the test chip is set in the micro reaction chamber, the micro reaction container has telescopic structure, the telescopic structure can be telescopic along the axial direction of the micro reaction container under the action of external force, so that the volume of the micro reaction chamber changes, and, the top of the micro reaction container is also provided with overflow groove and observation window, the observation window corresponds with the overflow groove, the overflow groove is communicated with the micro reaction chamber, the overflow detection mechanism is set in the overflow groove and located in the observation area of the observation window, the overflow detection mechanism can be changed after being contacted with the liquid phase system The change that can be observed visually.

[0021] Wherein, the liquid phase system includes the solution required by cell culture or biochemical reaction.

[0022] In some embodiments, the micro-reaction container comprises a container wall, a base fixedly arranged at the bottom of the container wall, and a top cover fixedly arranged at the top of the container wall, the container wall, the base and the top cover jointly form the micro-reaction chamber, and the container wall has the telescopic structure.

[0023] In some embodiments, the overflow detection mechanism comprises a test paper, and the visually observable change comprises a color change. For example, the color of the test paper changes from white to red when the test paper contacts the liquid.

[0024] In some embodiments, the container wall comprises a first container wall segment and a second container wall segment arranged in sequence along the axial direction of the micro-reaction container, the first container wall segment is fixedly connected with the base, and the second container wall segment has the telescopic structure.

[0025] In some preferred embodiments, the second container wall segment is a telescopic structure as a whole.

[0026] In some preferred embodiments, the telescopic structure is a corrugated structure.

[0027] In some preferred embodiments, the second container wall segment is a flexible corrugated structure.

[0028] In some preferred embodiments, the second container wall segment is a silica gel structure.

[0029] In some preferred embodiments, the base has a first receiving groove, a portion of the container wall is designed to be arranged in the first receiving groove, and the micro-reaction container further comprises a waterproof pressing sheet, the waterproof pressing sheet is arranged around the container wall, and a portion of the container wall is fixedly pressed between the waterproof pressing sheet and the base.

[0030] In some preferred embodiments, the base further has a second receiving groove, the second receiving groove is arranged at the groove bottom of the first receiving groove and is in communication with the first receiving groove, and the test chip is arranged in the second receiving groove.

[0031] In some preferred embodiments, the top cover is provided with a scale mark.

[0032] In some preferred embodiments, the top cover comprises a top cover part and a sleeve part, the sleeve part is sleeved outside the container wall, and the sleeve part is provided with a scale mark. According to the scale mark, the capacity can be adjusted more accurately.

[0033] In some preferred embodiments, the overflow groove is arranged on the top cover.

[0034] In some embodiments, the volume-adjustable cell culture and biochemical reaction detection device further comprises a volume adjusting mechanism, which is in transmission connection with the top cover and is used to drive the top cover to move along the axial direction of the micro-reaction container, so that the telescopic structure is telescoped.

[0035] In some preferred embodiments, the volume adjusting mechanism comprises a screw transmission assembly and an adjusting knob, which is arranged on the base.

[0036] In some preferred embodiments, the volume adjusting mechanism comprises a gear transmission assembly and an adjusting knob, which is arranged on the base.

[0037] In some embodiments, the volume-adjustable cell culture and biochemical reaction detection device further comprises a liquid storage tank, which is in communication with the overflow tank and is arranged on the top cover. The liquid overflowing from the micro-reaction container can flow into the liquid storage tank, so as to avoid the pollution of the experimental instruments and the operation table surface by the overflowing liquid.

[0038] In some embodiments, the base is provided with a groove, the bottom of which is a slope. The groove can facilitate the operator to push the device in and out of the mounting rail on the experimental equipment, and the slope of the groove facilitates the marking of the identification, such as the marking of different letters to distinguish whether the chip in the device is split type or integrated type.

[0039] In some embodiments, the bottom of the base is provided with a protruding structure, which is used to fix the base to the experimental equipment. The protruding structure can be a strip-shaped protruding structure located on both sides of the bottom of the base, which can be clamped into the corresponding mounting rail on the experimental equipment, so as to fix the device on the experimental equipment.

[0040] The technical scheme, its implementation process and principles will be further explained and described as follows in combination with the drawings and specific implementation cases.

[0041] Embodiments

[0042] Please refer to Figure 1 、 Figure 2 、 Figure 3 and Figure 4 , the utility model embodiment provides a volume-adjustable cell culture and biochemical reaction detection device, including micro -reaction container 1, test chip 2 and full overflow detection mechanism, the micro -reaction container 1 inside has the micro -reaction chamber for containing liquid phase system, test chip 2 is arranged in the micro -reaction chamber.

[0043] In the embodiment, the micro-reaction container comprises a container wall 101, a base 102 fixedly arranged at the bottom of the container wall 101, and a top cover 103 fixedly arranged at the top of the container wall 101, and the container wall 101, the base 102 and the top cover 103 jointly enclose the micro-reaction chamber.

[0044] In the embodiment, the container wall 101 comprises a first container wall segment and a second container wall segment arranged in sequence along the axial direction of the micro-reaction container, the first container wall segment is fixedly connected with the base 101, and the second container wall segment is a corrugated structure 1011. The container wall 101 is made of silica gel as a whole.

[0045] Please refer to Figure 5 , the base 102 has a first receiving groove, a part of the container wall 101 is designed in the first receiving groove, the micro-reaction container 1 further comprises a waterproof compression sheet 3 made of silica gel, the waterproof compression sheet 3 surrounds the container wall 101, and a part of the container wall 101 is fixedly compressed between the waterproof compression sheet 3 and the base 101. The base 101 further has a second receiving groove arranged at the bottom of the first receiving groove and in communication with the first receiving groove, and the test chip 2 is arranged in the second receiving groove.

[0046] In the embodiment, the top cover 103 comprises a top cover part and a sleeve part 104, the sleeve part 104 is sleeved outside the container wall 101, and the sleeve part 104 is provided with a scale mark; the top cover part is provided with an overflow groove and an observation window 4 corresponding to the overflow groove, the overflow groove is in communication with the micro-reaction chamber, a full-overflow detection mechanism is arranged in the overflow groove and located in the observation area of the observation window 4, and the full-overflow detection mechanism comprises a liquid immersion indicating test paper 5, the liquid immersion indicating test paper 5 can change color when contacting the liquid in the micro-reaction chamber, for example, the color of the liquid immersion indicating test paper changes from white to red when contacting the liquid.

[0047] The volume-adjustable cell culture and biochemical reaction detection device of the embodiment further comprises a volume adjusting mechanism in transmission connection with the top cover 103, the volume adjusting mechanism comprises a lead screw transmission assembly and an adjusting knob 6, the adjusting knob 6 is arranged on the base 102, and the top cover 103 can be driven to move up and down along the axial direction of the micro-reaction container 1 by rotating the adjusting knob 6 through the lead screw transmission assembly, so that the corrugated structure 1011 of the second container wall segment is stretched and contracted, the capacity of the micro-reaction container 1 can be changed, and the scale mark on the sleeve part 104 corresponds to display the corresponding volume of the micro-reaction chamber during the adjusting process. Please refer to Figure 6A schematic view of the volume-adjustable cell culture and biochemical reaction detection device of the present embodiment being adjusted to a certain volume state.

[0048] In the present embodiment, the top cover 103 is further provided with a liquid storage groove in communication with the overflow groove, which is arranged on the side of the top cover 103. When overflow occurs, the liquid immersion indicator paper first contacts the liquid to change color, and then the overflow liquid can flow into the liquid storage groove to avoid the overflow liquid from polluting the experimental instrument and the operating table.

[0049] The volume-adjustable cell culture and biochemical reaction detection device provided by the present embodiment is driven by the volume adjusting mechanism to make the top cover of the micro-reaction container move up and down, so that the corrugated structure of the container wall is stretched and contracted, thereby simply and conveniently adjusting the capacity of the micro-reaction container, and more suitable for actual use needs; by arranging the overflow detection mechanism, when the liquid overflows the upper edge of the container wall, the test paper on the top cover changes color after contacting the liquid, and the operator can conveniently observe and judge whether the liquid overflows through the observation window, which is beneficial to improve the experimental reliability.

[0050] Application Example 1

[0051] The present application example relates to a surface functionalization treatment method of a biological chip, which utilizes the volume-adjustable cell culture and biochemical reaction detection device disclosed in the present embodiment, so as to more efficiently solidify the detection antibody to the surface of the detection chip, and improve the sensitivity and accuracy of the detection of the hepatitis B surface antigen.

[0052] The biological activity treatment steps of the detection chip for detecting the hepatitis B surface antigen are as follows:

[0053] 1) Complete the assembly of the chip and the detection device: clean the surface of the chip with anhydrous ethanol to remove impurities on the surface. Assemble the cleaned chip in the volume-adjustable cell culture and biochemical reaction detection device, and adjust the initial volume of the micro-reaction cavity to 200 μL through the capacity adjusting knob.

[0054] 2) Biotin-SAM treatment of the chip: add 150 μL of Biotin-SAM solution with a concentration of 0.1 mmol / L to the micro-reaction cavity, and incubate at room temperature for 1 hour. (At this time, if the added liquid volume exceeds the set volume of the micro-reaction cavity, the liquid contacts the test paper, the test paper turns red when it meets water, indicating that this step fails, and the biological activity treatment process of the chip fails.)

[0055] 3) Washing: To avoid the liquid volume too small, the pipette tip too close to the chip surface detection area and improve the washing efficiency, the volume of the micro-reaction cavity is temporarily adjusted to 500 μL, and then washed with 5 times of anhydrous ethanol and 5 times of pure water. The washing step is to add 250 μL of anhydrous ethanol or pure water to the micro-reaction cavity, then suck out 250 μL and discard, and repeat the step.(The same as above, if the liquid volume added exceeds the set volume of the micro-reaction cavity, the liquid contacts the liquid immersion indicating test paper, the test paper turns red when it meets water, indicating that this step fails, and the bio-activation process of the chip fails.)

[0056] 4) Streptavidin treatment of the chip: The volume of the micro-reaction cavity is adjusted back to 200 μL again, 100 μL of streptavidin solution with a concentration of 150 μg / mL is added to the micro-reaction cavity, and incubated at room temperature for 30 minutes.(If the liquid volume added exceeds the set volume of the micro-reaction cavity, the liquid contacts the liquid immersion indicating test paper, the test paper turns red when it meets water, indicating that this step fails, and the bio-activation process of the chip fails.)

[0057] 5) Secondary washing: To avoid the liquid volume too small, the pipette tip too close to the chip surface detection area and improve the washing efficiency, the volume of the micro-reaction cavity is adjusted to 500 μL again, and then washed with 1X PBS for 10 times. The washing step is to add 250 μL of 1X PBS to the micro-reaction cavity, then suck out 250 μL and discard, and repeat the step.(The same as above, if the liquid volume added exceeds the set volume of the micro-reaction cavity, the liquid contacts the liquid immersion indicating test paper, the test paper turns red when it meets water, indicating that this step fails, and the bio-activation process of the chip fails.)

[0058] 6) Biotin-HbsAb treatment of the chip: The volume of the micro-reaction cavity is adjusted back to 200 μL, 100 μL of biotinylated hepatitis B surface antibody HbsAb with a concentration of 150 μg / mL is added to the micro-reaction cavity, and incubated at room temperature for 30 minutes.(If the liquid volume added exceeds the set volume of the micro-reaction cavity, the liquid contacts the liquid immersion indicating test paper, the test paper turns red when it meets water, indicating that this step fails, and the bio-activation process of the chip fails.)

[0059] 7) Final washing: In order to avoid the liquid volume being too small, the pipette tip being too close to the detection area on the chip surface, and improve the washing efficiency, the volume of the micro-reaction cavity is adjusted to 500 μL again, and the micro-reaction cavity is washed 10 times with 1X PBS. The washing step is to first add 250 μL of 1X PBS to the micro-reaction cavity, then suck out 250 μL and discard, and repeat this step.

[0060] 8) Sample detection: After the above steps are completed, the detection chip in the micro-reaction cavity has the ability to detect hepatitis B surface antigen. At this time, the volume of the micro-reaction cavity can be adjusted as needed, and the matching sample to be detected can be added. The capacitance change of the detection chip after adding the sample is detected in real time and recorded, and then it is inferred whether the antibody on the surface of the detection chip specifically binds to the antigen in the sample.

[0061] Application Example 2

[0062] The application example relates to a biological activity processing method of a biological chip for cell culture, which utilizes the cell culture and biochemical reaction detection device with adjustable volume disclosed in the utility model embodiment, can more efficiently solidify the protein suitable for cell adhesion to the chip surface, and realizes effective inoculation and culture of cells.

[0063] The biological activity processing and subsequent culture and detection experiment process of the detection chip for cell culture are as follows:

[0064] 1) Complete assembly of the chip and the detection device and sterilization: the chip surface is cleaned with anhydrous ethanol to remove impurities on the surface. The cleaned chip is assembled in the cell culture and biochemical reaction detection device with adjustable volume, the initial volume of the micro-reaction cavity is adjusted to 200 μL through the volume adjusting knob, and the assembled and adjusted test module is placed under the ultraviolet lamp for sterilization for 30 minutes.

[0065] 2) Protein solidification on the chip surface: according to the use instruction of the commercialized dithiobis(succinimidyl propionate) (DSP) crosslinking agent, the DSP is dissolved in DMSO at a certain proportion to prepare a solution, 150 μL of the solution is added to the micro-reaction cavity, and the solution is incubated at room temperature for 30 minutes.

[0066] Then adjust the volume of the micro-reaction chamber to 500 μL, wash with DMSO and pure water, 5 times each (10 times in total, the washing procedure is to add 250 μL DMSO or pure water to the micro-reaction chamber, then suck out 250 μL and discard, repeat the procedure).

[0067] Then adjust the volume of the micro-reaction chamber to 300 μL, add 250 μL sterile recombinant human fibronectin (FN) solution with a concentration of 100 μg / mL to the micro-reaction chamber, and incubate at room temperature or in a 37°C cell incubator for 4 hours. The disulfide atoms contained in the DSP can quickly chemisorb to the gold surface, and the active NHS groups at both ends can specifically bind to the primary amine groups in FN, ultimately allowing FN to solidify to the chip surface. Because the incubation time is long, more FN solution (250 μL) is used here.

[0068] Finally, adjust the volume of the micro-reaction chamber to 600 μL. Wash with sterile 1X PBS buffer for 10 times to remove the cross-linker byproducts and unbound proteins. (The washing procedure is to add 300 μL buffer to the micro-reaction chamber, then suck out 300 μL and discard, repeat the procedure.)

[0069] After carefully sucking out most of the washing buffer, adjust the volume of the test module to 200 μL, and let it stand for 30 minutes on the clean bench, and air dry the residual liquid in the micro-reaction chamber.

[0070] 3) Cell inoculation: After rinsing with basal medium, count the cells after resuspension with complete medium. In order to make the cells cover the metal surface of the detection capacitance chip as soon as possible, inoculate 100 μL at a density of 10 to the power of 4 / mL onto the detection capacitance chip, and let it stand in a 37°C cell incubator for 30 minutes to allow the cells to completely settle on the chip surface. Smaller micro-reaction chamber volume means lower liquid level, and cells suspended in solution are more likely to contact and adhere to the chip surface with FN protein attached.

[0071] 4) Cell culture: The volume of the cell culture medium added is determined according to the amount of cell inoculation and the culture time. After adjusting the volume of the micro-reaction chamber to the corresponding capacity (slightly higher than the volume of the culture medium) according to the volume of the culture medium, add an appropriate amount of culture medium to the micro-reaction chamber, and start the cell culture on the chip.

[0072] If the volume of the liquid added exceeds the set volume of the micro-reaction chamber in any of the above steps, the liquid contacts the liquid immersion indicator paper, and the paper turns red when it comes into contact with water, indicating that the step has failed.

[0073] In summary, the volume-adjustable cell culture and biochemical reaction detection device can flexibly adjust the volume according to the change requirement of the liquid volume in different steps of the cell culture and biological reaction process, adapt to various complex experimental conditions, and improve the experimental efficiency. The device can also provide failure safety protection capability, liquid overflow indication, flow guide and temporary storage, ensure the safety of the experimental personnel, and the legality and reliability of the experimental results.

[0074] From the specific experimental operations of application examples 1 and 2, it can be known that in the experimental operation process, it is usually necessary to repeatedly add and aspirate the solution. When the solution is added to the micro-reaction cavity by using a pipette gun, if the volume of the micro-reaction cavity is too large, the droplets of the solution are easy to drop to the side wall, so it is necessary to temporarily adjust the volume to adapt to the experimental requirements, and the size of the volume of the micro-reaction cavity and the volume of the reaction solution are more conducive to improving the chemical reaction efficiency. On the other hand, in the experimental cleaning operation, adjusting to a larger volume can add a larger volume of cleaning liquid at one time, so that the dilution is more sufficient, and the cleaning efficiency can be improved. Therefore, the volume-adjustable cell culture and biochemical reaction detection device developed by the present application can provide more flexible experimental conditions and more efficient cell culture or biological reaction detection, and has important practical significance and application value.

[0075] Although the present application has been described with reference to the illustrative embodiments, it will be understood by those skilled in the art that various other changes, omissions and / or additions can be made without departing from the spirit and scope of the present application. In addition, many modifications can be made to adapt a particular situation or material to the teachings of the present application without departing from the scope thereof. Therefore, it is intended that the present application not be limited to the disclosed embodiments for carrying out the present application, but rather that the present application be accorded the full scope inherent to the appended claims. Furthermore, unless specifically stated otherwise, any use of the terms first, second, etc., does not indicate any order or importance, but rather the terms first, second, etc., are used to distinguish one element from another.

[0076] It should be understood that the above embodiments are only to illustrate the technical concepts and characteristics of the present application, and the purpose is to enable those skilled in the art to understand the content of the present application and implement it, and cannot limit the protection scope of the present application. Any equivalent changes or modifications made in accordance with the spirit and essence of the present application should be covered within the protection scope of the present application.

Claims

1. A volume-adjustable cell culture and biochemical reaction detection device, characterized in that, The device includes a microreactor, a test chip, and an overflow detection mechanism. The microreactor has a microreactor chamber for holding a liquid system. The test chip is disposed within the microreactor chamber. The microreactor has a telescopic structure that can expand and contract along the axial direction of the microreactor under external force, thereby changing the volume of the microreactor chamber. The top of the microreactor also has an overflow channel and an observation window. The observation window corresponds to the overflow channel, which is connected to the microreactor chamber. The overflow detection mechanism is disposed within the overflow channel and located in the observation area of ​​the observation window. The overflow detection mechanism can undergo visually observable changes after contacting the liquid system.

2. The volume-adjustable cell culture and biochemical reaction detection device according to claim 1, characterized in that, The microreaction container includes a container wall, a base, and a top cover. The base is fixedly disposed at the bottom of the container wall, and the top cover is fixedly disposed at the top of the container wall. The container wall, the base, and the top cover together enclose the microreaction chamber, and the container wall has the telescopic structure.

3. The volume-adjustable cell culture and biochemical reaction detection device according to claim 1, characterized in that, The overflow detection mechanism includes test strips, and the visually observable changes include color changes.

4. The volume-adjustable cell culture and biochemical reaction detection device according to claim 2, characterized in that, The container wall includes a first container wall segment and a second container wall segment arranged sequentially along the axial direction of the microreaction container. The first container wall segment is fixedly connected to the base, and the second container wall segment has the telescopic structure.

5. The volume-adjustable cell culture and biochemical reaction detection device according to claim 4, characterized in that, The second container wall section is an overall retractable structure.

6. The volume-adjustable cell culture and biochemical reaction detection device according to claim 5, characterized in that, The stretchable structure is a corrugated structure.

7. The volume-adjustable cell culture and biochemical reaction detection device according to claim 5, characterized in that, The second container wall section has a flexible corrugated structure.

8. The volume-adjustable cell culture and biochemical reaction detection device according to claim 5, characterized in that, The second container wall section is made of silicone.

9. The volume-adjustable cell culture and biochemical reaction detection device according to claim 2, characterized in that, The base has a first receiving groove, a portion of the container wall is disposed within the first receiving groove, and the microreaction container further includes a waterproof clamping sheet surrounding the container wall, a portion of the container wall being clamped and fixed between the waterproof clamping sheet and the base.

10. The volume-adjustable cell culture and biochemical reaction detection device according to claim 9, characterized in that, The base also has a second receiving slot, which is disposed at the bottom of the first receiving slot and is in communication with the first receiving slot, and the test chip is disposed in the second receiving slot.

11. The volume-adjustable cell culture and biochemical reaction detection device according to claim 2, characterized in that, The top cover is equipped with scale markings.

12. The volume-adjustable cell culture and biochemical reaction detection device according to claim 2, characterized in that, The top cover includes a top cover portion and a sleeve portion, the sleeve portion being fitted onto the outside of the container wall, and the sleeve portion being provided with scale markings.

13. The volume-adjustable cell culture and biochemical reaction detection device according to claim 2, characterized in that, The overflow trough is provided on the top cover.

14. The volume-adjustable cell culture and biochemical reaction detection device according to claim 2, characterized in that, It also includes a volume adjustment mechanism, which is connected to the top cover and is used to drive the top cover to move axially along the microreactor, thereby causing the telescopic structure to extend and retract.

15. The volume-adjustable cell culture and biochemical reaction detection device according to claim 14, characterized in that, The volume adjustment mechanism includes a lead screw drive assembly and an adjustment knob, the adjustment knob being disposed on the base; Alternatively, the volume adjustment mechanism may include a gear transmission assembly and an adjustment knob, the adjustment knob being disposed on the base.

16. The volume-adjustable cell culture and biochemical reaction detection device according to claim 2, characterized in that, The top cover is provided with a liquid storage tank, which is connected to the overflow tank.

17. The volume-adjustable cell culture and biochemical reaction detection device according to claim 2, characterized in that, The base is provided with a groove, and the bottom of the groove is a slope.

18. The volume-adjustable cell culture and biochemical reaction detection device according to claim 2, characterized in that, The base has a protruding structure at its bottom, which is used to fix the base to the experimental equipment.

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