Organ-like perfusion culture chip

Abstract

The utility model relates to the technical field of organoid perfusion culture, and provides an organoid perfusion culture chip which comprises a box cover, a bottom box and a partition plate, the partition plate is fixedly connected to the interior of the bottom box, and a plurality of partition rings are fixedly connected to the inner wall of the partition plate; a plurality of mounting through holes are formed in the box cover, first culture barrels are inserted into the mounting through holes, and second culture barrels are inserted into the inner walls of the mounting through holes; sealing rings are fixedly connected to the outer walls of the first culture barrel and the second culture barrel. The bottom box is divided into an upper space and a lower space through the partition plate, the microporous membrane of the first culture barrel can be located in the space below the partition plate through the partition ring, the second culture barrel can be located in the space above the partition plate, and deviation of a final result caused by mutual interference when liquid is input into the culture box is avoided through the sealing ring.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the organoid perfusion culture technical field, concretely relates to an organoid perfusion culture chip. BACKGROUND

[0002] The organoid perfusion culture chip is a kind of biological chip that simulates the function of human organs in vitro using microfluidic technology.It realizes the culture and maintenance of organoids by constructing microfluidic channels and culture chambers on the chip, enabling them to grow and functionally express under conditions similar to in vivo environment.This chip is mainly used in medical research and drug development fields, such as disease mechanism research, drug action mechanism analysis, drug screening and toxicity testing, etc., and can also be applied to personalized medicine by culturing patient-derived organoids to test drug reactions, thus providing personalized treatment plans.

[0003] The authorized patent with application number 202222007680.2 discloses an organoid perfusion culture chip: including placing shell, the inside of placing shell is fixedly connected with support plate near the position of upper end, a plurality of limit grooves are opened in the inside of support plate, a plurality of connecting pipes are fixedly connected on one side of placing shell near the position of lower end.In the utility model, the culture medium is injected into the placing shell through the first liquid inlet pipe, the shunt pipe and the connecting pipe, enters the corresponding culture shell through the second liquid inlet pipe, the cell suspension is added to the partition ring for culture, the culture medium can enter the partition ring through the through hole to provide nutrients and oxygen for cells, when the liquid level in the culture shell exceeds the upper end of the cannula, the culture medium enters the liquid storage shell through the cannula and the first liquid outlet pipe, and finally is discharged through the second liquid outlet pipe, so that all culture medium storage tanks are uniformly perfused under the condition of reducing the sample interference in the culture shell.

[0004] However, in the implementation of the related technology, the above technical solution has the following problems: when using, if the internal liquid is too much, and the internal liquid is turbulent, the environment of cells is affected, and the culture in different size culture shells produces different environments, which easily leads to different changes of the same cells, resulting in deviation of the observed phenomenon.

[0005] Therefore, an organoid perfusion culture chip is proposed to solve the above problems. Utility model content

[0006] The utility model discloses an organoid perfusion culture chip, which solves the problem that in the related technology, when using, if the internal liquid is too much, and the internal liquid is turbulent, the environment of cells is affected, and the culture in different size culture shells produces different environments, which easily leads to different changes of the same cells, resulting in deviation of the observed phenomenon.

[0007] The technical scheme of the utility model discloses a kind of organoid perfusion culture chips, comprising: box cover, bottom box and baffle, the baffle is fixedly connected in the inside of bottom box, the inner wall of baffle is fixedly connected with multiple partition rings;

[0008] The inside of the box cover is provided with a plurality of mounting through holes, the inside of the mounting through hole is inserted with the first culture barrel, and the inner wall of the mounting through hole is inserted with the second culture barrel.

[0009] The outer wall of the first culture barrel and the second culture barrel is fixedly connected with a sealing ring.

[0010] Preferably, the outer side of the box cover is fixedly connected with a box along, the lower surface of the box cover is fixedly connected with an upper partition ring, and the first culture barrel is inserted into the inner wall of the upper partition ring.

[0011] Preferably, the end of the second culture barrel is inserted into the inner wall of the partition ring, and the bottom of the first culture barrel and the second culture barrel is fixedly connected with a microporous membrane.

[0012] Preferably, the bottom end of the first culture barrel is above the baffle, the second culture barrel penetrates the baffle, and the highest point of the partition ring and the highest point of the bottom box are at the same horizontal plane.

[0013] Preferably, the outer wall of the bottom box is fixedly connected with an upper output pipe, the outer wall of the bottom box is fixedly connected with an upper input pipe, the upper input pipe and the upper output pipe are located on the upper side of the baffle, the outer wall of the bottom box is fixedly connected with a lower input pipe, the other side of the bottom box is fixedly connected with a lower output pipe, and the lower input pipe and the lower output pipe are located below the baffle.

[0014] Preferably, the first culture barrel and the second culture barrel are staggered, and the height of the first culture barrel is higher than that of the second culture barrel.

[0015] The working principle and beneficial effects of the utility model are as follows:

[0016] By the setting of the baffle, the bottom box is divided into two spaces, by the setting of the partition ring, the microporous membrane of the first culture barrel can be located in the space below the baffle, the second culture barrel is located in the space above the baffle, and by the setting of the sealing ring, mutual interference is avoided when liquid is inputted inside, so that the final result is deviated. BRIEF DESCRIPTION OF DRAWINGS

[0017] The utility model will be further explained in detail in combination with drawings and specific embodiments.

[0018] Figure 1 The utility model proposes a three-dimensional structure schematic diagram;

[0019] Figure 2The utility model provides a box cover's three-dimensional structure schematic diagram is provided.

[0020] Figure 3 The utility model provides a first culture bucket's three-dimensional structure schematic diagram is provided.

[0021] Figure 4 The utility model provides a box cover's bottom view three-dimensional structure schematic diagram is provided.

[0022] Figure 5 The utility model provides a second culture bucket's section view three-dimensional structure schematic diagram is provided

[0023] Figure 6 The utility model provides a first culture bucket's section view three-dimensional structure schematic diagram is provided

[0024] In the drawing: 1, box cover;2, bottom box;3, baffle;4, lower input pipe;5, upper input pipe;6, partition ring;7, upper partition ring;8, box along;9, first culture bucket;10, second culture bucket;11, sealing ring;12, microporous membrane;13, lower output pipe;14, upper output pipe;15, mounting through hole. Specific implementation

[0025] The technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the embodiments of the utility model, and obviously, the described embodiments only are a part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the person skilled in the art without making creative labor are all related to the range of protection of the utility model.

[0026] Implementation

[0027] Please refer to Figure 1 -6, an organ-like perfusion culture chip, comprising: box cover 1, bottom box 2 and baffle 3, baffle 3 is fixedly connected in the inside of bottom box 2, and the inner wall of baffle 3 is fixedly connected with multiple partition rings 6;

[0028] The inside of box cover 1 is provided with multiple mounting through holes 15, the inside of mounting through hole 15 is inserted with first culture bucket 9, and the inner wall of mounting through hole 15 is inserted with second culture bucket 10;

[0029] The outer wall of first culture bucket 9 and second culture bucket 10 is fixedly connected with sealing ring 11.

[0030] The technical scheme provided by the embodiment is as follows: first, the box cover 1 is covered on the upper surface of the bottom box 2, the box edge 8 is clamped around the bottom box 2, so that the box cover 1 is relatively fixed, then the second culture barrel 10 is first installed through the installation through hole 15, the second culture barrel 10 is installed at the staggered position of the partition ring 6, when the second culture barrel 10 is installed, the sealing ring 11 on the outer wall of the second culture barrel 10 is in contact with the upper partition ring 7, so that the second culture barrel 10 is not in excessive contact with the outside world, then the first culture barrel 9 is inserted into the empty installation through hole 15, after the first culture barrel 9 penetrates the partition ring 6, the microporous membrane 11 at the bottom of the first culture barrel 9 is located in the space at the bottom of the partition plate 3, and the sealing ring 11 on the outer wall of the first culture barrel 9 is in contact with the partition ring 6, so that the space above the partition plate 3 is isolated from the space below the partition plate 3, after the installation is completed, the culture solution is input through the upper input pipe 5 and the lower output pipe 4, the culture solution is input to the upper and lower sides of the partition plate 3, and then the culture solution is output through the upper output pipe 14 and the lower output pipe 13, so that the liquid in the inside is circulated.

[0031] Further, the box cover 1 is fixedly connected with the box edge 8 on the outside, and the upper partition ring 7 is fixedly connected to the lower surface of the box cover 1, and the first culture barrel 9 is inserted into the inner wall of the upper partition ring 7.

[0032] Specifically, the partition ring 7 isolates the space in the bottom box 2 from the outside world, so that the external environment does not affect the internal environment during the culture.

[0033] Further, the end of the second culture barrel 10 is inserted into the inner wall of the partition ring 6, and the bottom of the first culture barrel 9 and the bottom of the second culture barrel 10 are fixedly connected with the microporous membrane 12.

[0034] Specifically, the partition ring 6 isolates the space on the upper and lower sides of the partition plate 3, so that the spaces on the two sides do not affect each other.

[0035] Further, the bottom end of the first culture barrel 9 is located above the partition plate 3, the second culture barrel 10 penetrates the partition plate 3, and the highest point of the partition ring 6 is at the same horizontal plane as the highest point of the bottom box 2.

[0036] Specifically, the highest point of the partition ring 6 is at the same horizontal plane as the highest point of the bottom box 2, so that the liquid in the upper space of the partition plate does not affect the lower space even when the upper space is full.

[0037] Further, the outer wall of the bottom box 2 is fixedly connected with the upper output pipe 14, the outer wall of the bottom box 2 is fixedly connected with the upper input pipe 5, the upper input pipe 5 and the upper output pipe 14 are located on the upper side of the partition plate 3, the outer wall of the bottom box 2 is fixedly connected with the lower input pipe 4, the other side of the bottom box 2 is fixedly connected with the lower output pipe 13, and the lower input pipe 4 and the lower output pipe 13 are located below the partition plate 3.

[0038] Specifically, the output and input pipes are arranged on the upper and lower sides, so that the spaces on the upper and lower sides of the partition plate can be independently circulated.

[0039] Further, the first culture barrel 9 and the second culture barrel 10 are staggered, and the height of the first culture barrel 9 is higher than that of the second culture barrel 10.

[0040] Specifically, the two culture barrels are arranged at different heights, so that the microporous membranes at the bottoms of the two culture barrels can be located in different spaces during operation.

[0041] The above is only a preferred embodiment of the present application, and is not intended to limit the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. An organoid perfusion culture chip, comprising: The box cover (1), the bottom box (2) and the partition plate (3) are characterized in that the partition plate (3) is fixedly connected to the inside of the bottom box (2), and the inner wall of the partition plate (3) is fixedly connected with a plurality of partition rings (6); A plurality of mounting through holes (15) are formed in the inside of the box cover (1), the first culture barrel (9) is inserted into the inside of the mounting through hole (15), and the second culture barrel (10) is inserted into the inner wall of the mounting through hole (15); The outer wall of the first culture barrel (9) and the second culture barrel (10) is fixedly connected with a sealing ring (11).

2. The organoid perfusion culture chip according to claim 1, characterized in that: The outer side of the box cover (1) is fixedly connected with a box rail (8), the lower surface of the box cover (1) is fixedly connected with an upper partition ring (7), and the first culture barrel (9) is inserted into the inner wall of the upper partition ring (7).

3. The organoid perfusion culture chip according to claim 1, wherein: The end of the second culture barrel (10) is inserted into the inner wall of the partition ring (6), and the bottom of the first culture barrel (9) and the second culture barrel (10) is fixedly connected with a microporous membrane (12).

4. The organoid perfusion culture chip according to claim 1, wherein: The bottom end of the first culture barrel (9) is located above the partition plate (3), the second culture barrel (10) penetrates the partition plate (3), and the highest point of the partition ring (6) and the highest point of the bottom box (2) are at the same horizontal plane.

5. The organoid perfusion culture chip according to claim 1, wherein: The outer wall of the bottom box (2) is fixedly connected with an upper output pipe (14), the outer wall of the bottom box (2) is fixedly connected with an upper input pipe (5), the upper input pipe (5) and the upper output pipe (14) are located on the upper side of the partition plate (3), the outer wall of the bottom box (2) is fixedly connected with a lower input pipe (4), the other side of the bottom box (2) is fixedly connected with a lower output pipe (13), and the lower input pipe (4) and the lower output pipe (13) are located below the partition plate (3).

6. The organoid perfusion culture chip according to claim 1, wherein: The first culture barrel (9) and the second culture barrel (10) are staggered, and the height of the first culture barrel (9) is higher than that of the second culture barrel (10).

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