Flow type antibody detection device

Abstract

The utility model discloses a flow antibody detection device, and relates to the technical field of detection devices.The flow antibody detection device comprises an operation panel, the operation panel is provided with a mixing assembly used for mixing an antibody and a fluorescent agent, the mixing assembly comprises a first motor installed on the upper surface of the operation panel, the output end of the first motor is fixedly connected with a rotating disc, and the rotating disc is provided with a clamping assembly; through cooperative arrangement of a rotating rod, a threaded rod, a sealing plate and other structures, during use, a moving block slides on the rotating rod, a moving ring sleeves a fixed rod through a sliding hole, then the rotating rod is rotated to enable the moving block to be in threaded connection with the threaded rod, and then the moving block is driven to descend; and the sealing plate is pressed above the vessel to seal the vessel, so that the problem of resource waste caused by easy spilling when the vessel is filled with too much water is avoided as much as possible.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of detection devices, in particular to a flow type antibody detection device. BACKGROUND

[0002] There is no uniform definition of cells, and a commonly used definition is that cells are basic structural and functional units of organisms. All known organisms except viruses are composed of cells, but viruses also need to be in cells to carry out life activities. Antibodies refer to proteins produced by the body due to stimulation by antigens and having a protective effect. Immunoglobulin is not only an antibody, but also a large Y-shaped protein secreted by plasma cells (effector B cells) and used by the immune system to identify and neutralize foreign substances such as bacteria and viruses. It is only found in the body fluids of vertebrates such as blood and the cell membrane surface of B cells. Antibodies can recognize a unique feature of a specific foreign object, which is called an antigen. When performing flow cytometry, antibodies are usually mixed with fluorescent agents and then added to the cell solution to be detected.

[0003] The utility model discloses a flow type antibody detection device, including: box, track, mobile seat, stirring subassembly and mixing subassembly, antibody table is set up with antibody cavity, stirring subassembly sets up in the antibody cavity, and the pump body is set up on the antibody table, and the first conduit one end is connected with the pump body input end, and the first conduit other end inserts in the antibody cavity.

[0004] The flow type antibody detection device in the above-mentioned document places a container containing antibodies and fluorescent agents on a stirring base for rotation and stirring, and then controls the working time of the pump body to achieve accurate control of the injection amount. However, when the container rotates, the mixture of antibodies and fluorescent agents in the container will rise on the inner wall of the container under the action of centrifugal force. When the container is filled too much, the mixture is easy to spill out, causing resource waste. Utility model content

[0005] The utility model discloses a flow type antibody detection device, including: box, track, mobile seat, stirring subassembly and mixing subassembly, antibody table is set up with antibody cavity, stirring subassembly sets up in the antibody cavity, and the pump body is set up on the antibody table, and the first conduit one end is connected with the pump body input end, and the first conduit other end inserts in the antibody cavity.

[0006] To achieve the above-mentioned purpose, the utility model adopts the following technical scheme:

[0007] The utility model provides a kind of flow antibody detection device, including operation board, which is equipped with mixing component for mixing antibody and fluorescent agent on the operation board, and the mixing component includes the first motor installed on the upper surface of the operation board, the first motor output end is fixedly connected with rotating disc, the rotating disc is equipped with clamping component, the upper surface of the operation board one end is fixedly connected with vertical support plate, the support plate one side side wall is equipped with sliding slot, the support plate top is equipped with rotating rod, the rotating rod one end is located in sliding slot and is fixedly connected with threaded rod, the threaded rod end away from the rotating rod is rotatably connected with the sliding slot, rotating rod is equipped with moving block, the moving block is screw-connected with the threaded rod, the moving block one side side wall is hinged with moving ring, the moving ring is rotatably connected with sealing plate, the sealing plate is located directly above the rotating disc, the upper surface of the operation board is fixedly connected with annular array of fixed rod in the four sides of the first motor, the moving ring is equipped with four sliding holes that are matched with the fixed rod respectively.

[0008] By adopting the above technical scheme, when in use, the user places the vessel containing the antibody and the fluorescent agent on the rotating disc, fixes it with the clamping component, then slides the moving block on the rotating rod, sleeves the moving ring on the fixed rod through the sliding hole, then rotates the rotating rod while pressing the moving ring, so that the moving block is screw-connected with the threaded rod and then drives the moving block to descend, so that the sealing plate is pressed above the vessel to seal the vessel, then starts the first motor to drive the rotating disc to rotate, so that the vessel rotates with the sealing plate to mix the antibody and the fluorescent agent, which can avoid the problem that the resource is wasted due to spilling when the vessel contains too much.

[0009] Optionally, the clamping component includes a guide groove formed on the upper surface of the rotating disc, and a bidirectional screw rod is rotatably connected in the guide groove, and two oppositely arranged arc-shaped clamping blocks are screw-connected on the bidirectional screw rod.

[0010] By adopting the above technical scheme, after placing the vessel containing the antibody and the fluorescent agent on the rotating disc, the two arc-shaped clamping blocks are brought close to each other and abut against the vessel by rotating the bidirectional screw rod, so that vessels of different sizes can be fixed on the rotating disc to prevent the vessel from shaking on the rotating disc.

[0011] Optionally, a support frame is fixedly connected to the upper surface of the operation board, a pump body is mounted on the upper surface of the support frame, a first hose for extracting the mixture of the antibody and the fluorescent agent is fixedly connected to the input end of the pump body, and an extrusion tube for injecting the mixture into the cell solution is fixedly connected to the output end of the pump body through a pipeline penetrating through the support frame.

[0012] Through the above technical scheme, after the antibody and the fluorescent agent are mixed, the first hose is controlled to accurately extract the antibody and the fluorescent agent mixture in the container, and then the mixture is discharged to the container containing the cell liquid through the extrusion pipe, so that the injection amount is accurately controlled.

[0013] Optionally, one end of the upper surface of the operation plate is fixedly connected with a detection table, a guide rail is fixedly connected below the detection table and the support frame, an operation box is slidably arranged on the guide rail, a second motor is installed in the operation box, and a mixing seat is fixedly connected to the output end of the second motor.

[0014] Through the above technical scheme, the container containing the cell liquid is placed on the mixing seat, then the operation box is moved to below the support frame through the guide rail, the antibody and the fluorescent agent are added to the cell liquid, then the second motor is started to mix the cell liquid and the antibody uniformly, and then the operation box is moved to below the detection table, so that the operation box is conveniently detected.

[0015] Optionally, the lower surface of the sealing plate is fixedly connected with a second hose, the upper surface of the sealing plate is fixedly connected with a connecting pipe in communication with the second hose, and the first hose is inserted into the connecting pipe.

[0016] Through the above technical scheme, after the antibody and the fluorescent agent are mixed, the first motor is turned off, then the second hose is inserted into the connecting pipe, and the mixed antibody and fluorescent agent in the container can be directly extracted, which is convenient and fast.

[0017] Optionally, the side wall of the arc-shaped clamping block on the side close to each other is fixedly connected with a rubber plate.

[0018] Through the above technical scheme, the rubber plate with elasticity can improve the stability of clamping the container and prevent damage to the container.

[0019] Optionally, the top end of the fixed rod is higher than the top end of the threaded rod.

[0020] Through the above technical scheme, when the moving block is threadedly connected with the threaded rod, the moving ring is first sleeved on the fixed rod through the sliding hole, so that the moving block is prevented from shaking and the moving ring is not inconveniently sleeved on the fixed rod.

[0021] Optionally, a plurality of annularly arranged notches are formed in the side wall of one end of the second hose away from the sealing plate.

[0022] Through the above technical scheme, the second hose bottom end can be prevented from abutting against the bottom or the side wall of the container, so that the second hose is not blocked.

[0023] In summary, the beneficial effects of the present application are as follows:

[0024] 1. The application is set by the cooperation between the rotating rod, threaded rod and sealing plate structure, in use, the user will be placed in the rotating disc with antibody and fluorescent agent, make clamping assembly to its fixed, then the moving block in the rotating rod sliding, make moving ring through the sliding hole on the fixed rod, then press the moving ring while rotating the rotating rod, make the moving block and threaded rod threaded connection after driving the moving block down, make the sealing plate pressure on the top of the vessel, the vessel is sealed, then start the first motor driving rotating disc rotation, make the vessel and sealing plate rotation, mix the antibody and fluorescent agent, try to avoid when holding too much easy to spill, resulting in waste of resources problem;

[0025] 2. The vessel is placed in the rotating disc with antibody and fluorescent agent, through the rotation of the two-way screw, make two arc clamping block close to each other with the vessel, can be fixed in different size of the vessel on the rotating disc, prevent the vessel on the rotating disc shaking. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 It is the whole structure schematic diagram of the utility model;

[0027] Figure 2 It is the rotating disc structure schematic diagram of the utility model;

[0028] Figure 3 It is the Figure 1 A area amplification structure schematic diagram of the utility model;

[0029] Figure 4 It is the operation box internal structure schematic diagram of the utility model.

[0030] Mark explanation: 1, operation board;2, first motor;3, rotating disc;4, support plate;5, sliding slot;6, rotating rod;7, threaded rod;8, moving block;9, moving ring;10, sealing plate;11, fixed rod;12, sliding hole;13, guide slot;14, two-way screw;15, arc clamping block;16, support frame;17, pump body;18, first hose;19, extrusion pipe;20, detection table;21, guide rail;22, operation box;23, second motor;24, mixing seat;25, second hose;26, connecting pipe;27, rubber plate;28, notch. DETAILED DESCRIPTION

[0031] The following will be combined with the Figures 1-4 Make further detailed description to the application.

[0032] Please refer to Figure 1The utility model provides a kind of flow antibody detection device, including operating plate 1, and be equipped with the mixing component for mixing antibody and fluorescent agent on operating plate 1, and the mixing component includes first motor 2, rotating disc 3, support plate 4, sliding slot 5, rotating rod 6, threaded rod 7, moving block 8, moving ring 9, sealing plate 10, fixed rod 11 and sliding hole 12;

[0033] First motor 2 is installed on the upper surface of operating plate 1, rotating disc 3 is fixedly connected at the output end of first motor 2, for placing vessel, mixing antibody and fluorescent agent, rotating disc 3 is equipped with clamping component for fixing different size vessels on rotating disc 3, support plate 4 is fixedly connected on one end of the upper surface of operating plate 1 and is vertically arranged, sliding slot 5 is opened on the side wall of support plate 4 close to first motor 2, rotating rod 6 is located at the top of support plate 4 and one end is located in sliding slot 5 and is fixedly connected with threaded rod 7, rotating rod 6 is rotatably connected with support plate 4 and the top is fixedly connected with rotating block, threaded rod 7 is rotatably connected with the bottom wall of sliding slot 5 away from rotating rod 6, moving block 8 is sleeved on rotating rod 6 and is threadedly connected with threaded rod 7, moving ring 9 is hinged on one side wall of moving block 8, sealing plate 10 is located in the interior of moving ring 9 and forms rotating connection, sealing plate 10 is located directly above rotating disc 3, fixed rod 11 is fixedly connected on the upper surface of operating plate 1 and is located at the four sides of first motor 2 and forms annular array distribution, sliding hole 12 is provided with four and is opened on the upper surface of moving ring 9 respectively and is matched with fixed rod 11 respectively.

[0034] When using, the user places the vessel containing antibody and fluorescent agent on rotating disc 3, makes clamping component fixed to it, then sliding moving block 8 on rotating rod 6, makes moving ring 9 through sliding hole 12 on fixed rod 11, then press moving ring 9 while rotating rotating rod 6, makes moving block 8 and threaded rod 7 threadedly connected and then drives moving block 8 to descend, makes sealing plate 10 press on the upper side of vessel, seals the vessel, then starts first motor 2 and drives rotating disc 3 to rotate, makes vessel and sealing plate 10 rotate together, mixes antibody and fluorescent agent, avoids the problem that resource is wasted when containing too much and spilling out.

[0035] Refer to Figure 2 Clamping component includes guide groove 13 opened on the upper surface of rotating disc 3, and two-way screw rod 14 is rotatably connected in guide groove 13, one end of two-way screw rod 14 penetrates the side wall of guide groove 13 and is fixedly connected with rotating disc, two-way screw rod 14 is threadedly connected with two oppositely arranged arc-shaped clamping blocks 15, after placing the vessel containing antibody and fluorescent agent on rotating disc 3, rotating two-way screw rod 14 through rotating disc, makes two arc-shaped clamping blocks 15 approach each other and abut against vessel, can fix different size vessels on rotating disc 3, prevent vessel from shaking on rotating disc 3.

[0036] Refer to Figure 1The upper surface of the operation plate 1 is fixedly connected with a support frame 16, the upper surface of the support frame 16 is provided with a pump body 17, the input end of the pump body 17 is fixedly connected with a first hose 18 for extracting the mixture of the antibody and the fluorescent agent, the output end of the pump body 17 penetrates the support frame 16 through a pipeline and is fixedly connected with an extrusion pipe 19 for injecting the mixture into the cell liquid, after the antibody and the fluorescent agent are mixed, the first hose 18 accurately extracts the mixture of the antibody and the fluorescent agent in the container by controlling the working time of the pump body 17, and then the extrusion pipe 19 is used to discharge the mixture into the container containing the cell liquid, so that the injection amount is accurately controlled.

[0037] With reference to Figure 1 And Figure 4 One end of the upper surface of the operation plate 1 is fixedly connected with a detection table 20, the detection table 20 is provided with a flow cytometry antibody detector for detecting the cell liquid containing the antibody and the fluorescent agent, the flow cytometry antibody detector is provided with a laser excitation module and a fluorescence detection module, the laser excitation module comprises a stable laser and a high-precision optical fiber, and is used for generating a stable and uniform laser beam to excite the fluorescent antibody marked on the cell. The fluorescence detection module comprises a high-efficiency photoelectric detector and a signal processing unit, which can quickly and accurately collect and process the fluorescence signal. The flow cytometry antibody detector is a public technology, and therefore will not be described in detail. The upper surface of the operation plate 1 is fixedly connected with a guide rail 21 below the detection table 20 and the support frame 16, the operation box 22 is slidably arranged on the guide rail 21, the second motor 23 is installed in the operation box 22, the second motor 23 is fixedly connected with a mixing seat 24 at the output end, the container containing the cell liquid is placed on the mixing seat 24, then the operation box 22 is moved to below the support frame 16 through the guide rail 21, so that the antibody and the fluorescent agent are added to the cell liquid, then the second motor 23 is started to mix the cell liquid and the antibody uniformly, and then the operation box 22 is moved to below the detection table 20, so that the cell liquid can be conveniently detected.

[0038] With reference to Figure 1 The lower surface of the sealing plate 10 is fixedly connected with a second hose 25, the upper surface of the sealing plate 10 is fixedly connected with a connecting pipe 26 in communication with the second hose 25, and the first hose 18 is inserted into the connecting pipe 26. After the antibody and the fluorescent agent are mixed, the first motor 2 is closed, then the second hose 25 is inserted into the connecting pipe 26, and the mixture of the antibody and the fluorescent agent in the container can be directly extracted, which is convenient and fast.

[0039] With reference to Figure 2 The side wall of the arc-shaped clamping block 15 on the side close to each other is fixedly connected with a rubber plate 27. The elastic rubber plate 27 can improve the stability of clamping the container and prevent the container from being damaged.

[0040] With reference to Figure 1The top end of the fixed rod 11 is higher than the top end of the threaded rod 7, so that when the moving block 8 is threadedly connected with the threaded rod 7, the moving ring 9 is first sleeved on the fixed rod 11 through the sliding hole 12, preventing the moving block 8 from shaking and making it inconvenient to sleeve the moving ring 9 on the fixed rod 11.

[0041] With reference to Figure 3 The second hose 25 is provided with a plurality of annularly arranged notches 28 on the side wall of the end away from the sealing plate 10, so that the bottom end of the second hose 25 is prevented from abutting against the bottom or the side wall of the vessel, thereby preventing the second hose 25 from being blocked.

[0042] The implementation principle of the present application is as follows: when in use, the user places the vessel containing the antibody and the fluorescent agent on the rotating disc 3, so that the clamping assembly fixes the vessel, then slides the moving block 8 on the rotating rod 6, so that the moving ring 9 is sleeved on the fixed rod 11 through the sliding hole 12, then rotates the rotating rod 6 while pressing the moving ring 9, so that the moving block 8 is threadedly connected with the threaded rod 7 and then drives the moving block 8 to descend, so that the sealing plate 10 is pressed on the top of the vessel, thereby sealing the vessel, then starts the first motor 2 to drive the rotating disc 3 to rotate, so that the vessel rotates together with the sealing plate 10, thereby mixing the antibody and the fluorescent agent, and the problem of spilling when the vessel contains too much liquid and causing resource waste is avoided.

[0043] The above only describes the preferred embodiments of the present application and is not used to limit the present application, although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments or equivalently replace some of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A flow antibody detection device, comprising an operation plate (1) provided with a mixing assembly for mixing an antibody with a fluorescent agent, characterized in that: The mixing assembly includes a first motor (2) mounted on the upper surface of the operation plate (1), the output end of the first motor (2) is fixedly connected with a rotating disc (3), the rotating disc (3) is provided with a clamping assembly, one end of the upper surface of the operation plate (1) is fixedly connected with a vertical support plate (4), the side wall of one side of the support plate (4) is provided with a sliding groove (5), the top of the support plate (4) is provided with a rotating rod (6), one end of the rotating rod (6) is located in the sliding groove (5) and is fixedly connected with a threaded rod (7), the end of the threaded rod (7) away from the rotating rod (6) is rotatably connected with the sliding groove (5), the rotating rod (6) is provided with a moving block (8), the moving block (8) is threadedly connected with the threaded rod (7), the side wall of one side of the moving block (8) is hingedly connected with a moving ring (9), the moving ring (9) is rotatably connected with a sealing plate (10), the sealing plate (10) is located directly above the rotating disc (3), the upper surface of the operation plate (1) is fixedly connected with a circular array of fixed rods (11) on the four sides of the first motor (2), the moving ring (9) is provided with four sliding holes (12) which are matched with the fixed rods (11).

2. The flow-through antibody detection device of claim 1, wherein: The clamping assembly includes a guide groove (13) opened in the upper surface of the rotating disc (3), the guide groove (13) is rotatably connected with a bidirectional screw rod (14), the bidirectional screw rod (14) is threadedly connected with two oppositely arranged arc-shaped clamping blocks (15).

3. A flow-through antibody detection device according to claim 2, wherein: The upper surface of the operation plate (1) is fixedly connected with a support frame (16), the upper surface of the support frame (16) is mounted with a pump body (17), the input end of the pump body (17) is fixedly connected with a first hose (18) for extracting the mixture of the antibody and the fluorescent agent, the output end of the pump body (17) penetrates the support frame (16) through a pipeline and is fixedly connected with an extrusion pipe (19) for injecting the mixture into the cell solution.

4. The flow-through antibody detection device of claim 3, wherein: One end of the upper surface of the operation plate (1) is fixedly connected with a detection table (20), the upper surface of the operation plate (1) is fixedly connected with a guide rail (21) below the detection table (20) and the support frame (16), the guide rail (21) is slidably provided with an operation box (22), the operation box (22) is mounted with a second motor (23), the output end of the second motor (23) is fixedly connected with a mixing seat (24).

5. A flow-through antibody detection device according to claim 4, wherein: The lower surface of the sealing plate (10) is fixedly connected with a second hose (25), the upper surface of the sealing plate (10) is fixedly connected with a connecting pipe (26) which is in communication with the second hose (25), the first hose (18) is inserted into the connecting pipe (26).

6. A flow-through antibody detection device according to claim 5, wherein: The side wall of one side of the arc-shaped clamping block (15) is fixedly connected with a rubber plate (27).

7. A flow-through antibody detection device according to claim 6, wherein: The top end of the fixed rod (11) is higher than the top end of the threaded rod (7).

8. A flow-through antibody detection device according to claim 7, wherein: A plurality of annular array of notches (28) are formed in the side wall of one end of the second hose (25) away from the sealing plate (10).

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