Detection box for small molecule drug detection

Abstract

The utility model relates to a detection box for small molecule drug detection. The detection box comprises a box body and an upper cover, a mounting plate is arranged at the upper end of the box body; an insulating module is arranged on the box body; a liquid storage cup is arranged in the middle of the mounting plate; a liquid supply chamber is arranged in the liquid storage cup; a dialysis membrane is further arranged on the side wall of the liquid storage cup; a stirring assembly capable of stirring liquid in the liquid supply chamber is arranged on the lower surface of the upper cover; the detection box for small molecule drug detection is ingenious in design, after liquid enters the liquid storage cup on the mounting plate, the stirring assembly on the lower surface of the upper cover is inserted into the liquid storage cup, at the moment, the stirring assembly is started, the stirring assembly stirs the liquid in the liquid supply chamber, and it is ensured that small molecules in drugs are evenly distributed in the liquid supply chamber; the stability and repeatability of detection signals are improved, so that the reproducibility and accuracy of detection are improved, and then the detection signals uniformly enter the first compartment through the dialysis membrane; through combined use of stirring and dialysis membranes, local difference of drug concentration in a sample can be reduced, and detection deviation is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of drug concentration detection, more particularly to a detection box for small molecule drug detection. BACKGROUND

[0002] Small molecule drugs (Small Molecule Drugs) usually refer to relatively small molecular weight organic compounds, which have pharmacological activity and can be used for preventing, diagnosing and treating diseases; in order to ensure drug safety, effectiveness and quality control, it is very important to detect the existence, concentration and characteristics of small molecule drugs, which is of great significance for drug research and development, production, clinical application and supervision;

[0003] Patent No. CN202222177502.4 discloses a detection box for small molecule drug detection, as shown in Figure 1 The vessel 1 is divided into two parts by the insulating film 2, one of which is the cis compartment 101, and the other of which is the trans compartment 102; it also includes a nanopore 3 inlaid on the insulating film 2, which connects the cis compartment 101 and the trans compartment 102; it also includes a discharge device 5 for applying voltage to the electrolyte, the negative electrode of the discharge device 5 is arranged in the cis compartment 101, and the positive electrode is arranged in the trans compartment 102; it also includes a recording device 4 for recording the electrical signal of the nanopore 3; it also includes a body fluid supply unit, which includes a liquid supply channel 6, the liquid supply channel 6 includes an input port 22 and an output port 24, the input port 22 is used for receiving the flow of body fluid into the liquid supply channel 6, and the output port 24 is used for discharging the body fluid from the liquid supply channel 6, the liquid supply channel 6 is made of a semi-permeable membrane material.

[0004] In the above structure, the liquid supply channel 6 is vertically placed in the U-shaped structure in the vessel 1, and there is no stirring assembly in the device to stir the movement of the medicine in the liquid supply channel 6, which will cause the small molecule drugs to gather at the bottom of the U-shaped liquid supply channel, and the liquid concentration in the upper part of the U-shaped liquid supply channel is less than that in the lower part; resulting in uneven distribution of small molecule drugs in the U-shaped channel, which may result in different electrical signals obtained each time, which will reduce the reproducibility of the detection and the accuracy of the detection. Utility model content

[0005] The utility model solves the technical problem in view of the above defects of prior art, and provides a detection box for small molecule drug detection.

[0006] The utility model discloses a detection box for small molecule drug detection, which comprises a box body with an open upper end, an upper cover detachably connected with the box body, an installation plate provided at the upper end of the box body to open or close the opening, a containing cavity formed by the box body and the installation plate, an insulation module provided in the containing cavity of the box body, the containing cavity being divided into a first compartment and a second compartment by the insulation module, a liquid storage cup provided in the middle of the region of the first compartment and recessed downward and extending towards the bottom of the box body, a liquid supply chamber provided in the liquid storage cup, a through hole provided on the side wall of the liquid storage cup and communicating with the liquid supply chamber and the first compartment, a dialysis membrane fixedly covered on the through hole, and a stirring assembly provided on the lower surface of the upper cover and corresponding to the liquid storage cup to stir the liquid in the liquid supply chamber.

[0007] The detection box further comprises guiding grooves provided on the inner walls of the opposite sides of the box body, a support plate matched and clamped with the two guiding grooves, and a sealing element tightly arranged between the outer side edge of the support plate and the guiding grooves.

[0008] The detection box further comprises a plurality of insulation membranes provided on the middle part of the installation plate to separate the electrolyte solution in the first compartment and the second compartment and allow the ions in the first compartment to enter the second compartment.

[0009] The detection box further comprises the through hole and the dialysis membrane, which are both provided on the side wall of the liquid storage cup.

[0010] The detection box further comprises a stirring rod rotatably connected with the upper cover through a flange bearing and a rotating motor for driving the self-rotation of the stirring rod, and a plurality of stirring blades circumferentially provided on the outer surface of the stirring rod along the length of the stirring rod.

[0011] The detection box further comprises a liquid inlet channel and a liquid outlet channel provided above the liquid storage cup on the upper cover, wherein the liquid inlet channel and the liquid outlet channel both extend into the liquid in the liquid supply chamber, and the liquid inlet channel and the liquid outlet channel are both in communication with the liquid supply chamber in the liquid storage cup.

[0012] The detection box further comprises the liquid inlet channel and the liquid outlet channel, which are respectively located on the two sides of the stirring assembly, and the lower end height of the liquid inlet channel is lower than the lower end height of the liquid outlet channel.

[0013] The utility model discloses a detection box, wherein the box body is internally provided with a sealed cavity on one side of the accommodating cavity; a discharge assembly for conducting electricity to the electrolyte solution in the first compartment and the second compartment is sealingly arranged in the sealed cavity.

[0014] The utility model discloses a detection box, wherein the outer edge of the upper surface of the mounting plate is circumferentially provided with a positioning ring protruding upward; the lower surface of the upper cover is provided with a positioning groove corresponding to and cooperatively inserted with the positioning ring.

[0015] The utility model discloses a detection box, wherein the volume of the first compartment is not less than the volume of the second compartment.

[0016] The utility model discloses a detection box for small molecule drug detection, which has the advantages that the liquid enters the liquid storage cup on the mounting plate, and then the box body and the upper cover are closed, the stirring assembly on the lower surface of the upper cover is inserted into the liquid storage cup, at this time, the stirring assembly is started, the liquid in the liquid supply chamber is stirred by the stirring assembly, ensuring that the small molecules in the drug are uniformly distributed in the liquid supply chamber, which helps to improve the stability and repeatability of the detection signal, thereby improving the reproducibility and accuracy of the detection, and then the liquid uniformly enters the first compartment through the dialysis membrane; and the combination of stirring and the dialysis membrane can reduce the local difference of the drug concentration in the sample and reduce the detection deviation. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the present application will be further described below with reference to the drawings and embodiments. The drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained without creative labor on the basis of these drawings:

[0018] Figure 1 It is a structure schematic view of a detection box for small molecule drug detection in the background art;

[0019] Figure 2 It is a structure schematic view of a detection box for small molecule drug detection in the preferred embodiment of the utility model;

[0020] Figure 3 It is Figure 2 A structure schematic view of the insulation module 31 in the background art.

[0021] Among them, the reference signs are as follows:

[0022] 1-vessel, 2-insulating film, 3-nanopore, 4-recording device, 5-discharge device, 6-liquid supply channel (detection tube), 7-dialysis membrane, 8-input catheter, 9-output catheter, 10-catheter, 11-catheter, 12-three-way joint, 13-valve, 22-input port, 24-output port, 101-cis compartment, 102-trans compartment;

[0023] 30-cassette, 31-insulating module, 311-support plate, 312-seal, 313-insulating film, 32-first compartment, 33-second compartment, 34-sealing cavity, 35-discharge assembly, 40-upper cover, 41-stirring assembly, 411-stirring rod, 412-rotary motor, 413-stirring blade, 42-liquid inlet channel, 43-liquid outlet channel, 50-mounting plate, 51-liquid storage cup, 52-liquid supply chamber, 53-dialysis membrane, 54-positioning ring. DETAILED DESCRIPTION

[0024] The terms "first", "second", "third", and "fourth" and the like in the description and in the claims of the present application and the accompanying drawings are used for distinguishing between similar objects, not necessarily described in a particular order. Also, the terms "comprise", "comprising", "including", and "having" and any variations thereof are intended to cover a non-exclusive inclusion, such that a process, method, system, product, or apparatus that comprises a list of steps or units are not necessarily limited to the steps or units that are expressly listed, but can optionally include additional steps or units that are not expressly listed or can also include steps or units inherent to such process, method, product, or apparatus.

[0025] Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are they necessarily mutually exclusive of one another. As will be apparent to those of ordinary skill in the art, embodiments described herein can be combined with other embodiments.

[0026] "Multiple" means two or more. "And / or", describing the association relationship of associated objects, means that there can be three relationships, for example, A and / or B can represent the three cases of A alone, A and B together, and B alone. The character " / " generally represents that the front and rear associated objects are in an "or" relationship.

[0027] Moreover, the terms "upper", "lower", "front", "back", "left", "right", "upper end", "lower end", "vertical", and the like indicating the orientation are all with reference to the attitude position of the device or equipment described in the scheme in normal use.

[0028] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, a clear and complete description will be provided below in conjunction with the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the protection scope of the present invention.

[0029] A preferred embodiment of this utility model provides a detection kit for small molecule drug detection, such as... Figures 2-3 As shown, a detection box for small molecule drug detection includes a box body 30 with an open top and a top cover 40 detachably connected to the box body 30. The upper end of the box body 30 is provided with a mounting plate 50 that can be opened or closed. In this embodiment, the mounting plate 50 is detachably connected to the box body 30 for easy cleaning or replacement. The box body 30 and the mounting plate 50 enclose a receiving cavity. An insulating module 31 is provided on the box body 30 within the receiving cavity. The insulating module 31 divides the receiving cavity into a first compartment 32 and a second compartment 33. A liquid storage cup 51, recessed downwards and extending towards the bottom of the box body 30, is provided in the middle of the region of the first compartment 32 on the mounting plate 50. In this embodiment, the liquid storage cup 51 has a cylindrical structure. The liquid storage cup 51 contains a... The liquid chamber 52; the side wall of the liquid storage cup 51 is also provided with a through hole (not shown in the figure) connecting the liquid supply chamber 52 and the first compartment 32, and a dialysis membrane 53 fixedly covering the through hole (not shown in the figure); small molecule drugs can be effectively transferred from the liquid supply chamber 52 to the first compartment 32 through the dialysis membrane 53, which speeds up the detection process; in this embodiment, the material of the dialysis membrane can be any synthetic polymer, such as polysulfone (PS), polyethersulfone (PES), polyacrylonitrile (PAN), modified cellulose material, etc., and optionally, multiple through holes are provided, and the liquid flow rate is fast; the lower surface of the upper cover 40 is provided with a stirring component 41 corresponding to the liquid storage cup 51 and capable of stirring the liquid in the liquid supply chamber 52.

[0030] This detection kit for small molecule drug detection is ingeniously designed. After the liquid enters the reservoir on the mounting plate, the kit body and top cover are closed. The stirring component on the lower surface of the top cover is inserted into the reservoir. At this time, the stirring component is activated, stirring the liquid in the supply chamber to ensure that the small molecules in the drug are evenly distributed in the supply chamber. This helps to improve the stability and repeatability of the detection signal, thereby improving the reproducibility and accuracy of the detection. Then, the drug is evenly introduced into the first compartment through the dialysis membrane. Furthermore, the combined use of stirring and the dialysis membrane can reduce local differences in drug concentration in the sample and reduce detection bias.

[0031] Further, the box body 30 is provided with a guide groove (not shown in the figure) on the inner wall of the opposite side; the insulation module is detachably connected with the box body 30, so that the insulation module can be quickly replaced and installed after detection, facilitating maintenance and upgrading; specifically, the insulation module 31 includes a support plate 311 matched and clamped with the two guide grooves (not shown in the figure), and a sealing element 312 closely arranged between the outer side edge of the support plate 311 and the guide groove; the sealing element 312 is located between the outer side edge of the support plate 311 and the guide groove, which can provide good sealing effect and prevent the electrolyte solution from leaking between the first compartment and the second compartment; the middle part of the mounting plate 50 is provided with an insulation diaphragm 313 fixedly arranged, which is used to separate the electrolyte solution in the first compartment 32 and the second compartment 33, and allows the ions in the first compartment 32 to pass into the second compartment 33, while other macromolecules or impurities are blocked, which helps to control the electrolyte environment; by controlling the flow of ions, the conditions of electrochemical detection can be optimized, and the sensitivity and stability of the detection signal can be improved.

[0032] Further, the insulation diaphragm 313 is provided with a plurality of; the flow of ions between the first compartment and the second compartment is accelerated; the plurality of insulation diaphragms 313 are all in the shape of a long strip and are vertically arranged along the length direction of the mounting plate 50; the effective area of ion transmission is increased, which helps to improve the ion transmission rate, so as to possibly improve the detection speed.

[0033] Further, the through hole (not shown in the figure) and the dialysis diaphragm 53 are both located on the side wall of the liquid storage cup 51.

[0034] Further, the stirring assembly 41 includes a stirring rod 411 rotatably connected with the upper cover 40 through a flange bearing (not shown in the figure), and a rotating motor 412 driving the stirring rod 411 to rotate; the outer surface of the stirring rod 411 is provided with a plurality of stirring blades 413 along the length direction thereof, in the embodiment, at least a part of the stirring rod and the stirring blades are inserted into the liquid in the liquid supply chamber, which can prevent the medicine or reaction product from precipitating at the bottom of the liquid supply chamber during the stirring process of the stirring rod and the stirring blades, and helps to reduce the dead angle in the stirring process, ensures that the liquid in the entire liquid supply chamber can be fully stirred, and ensures that the small molecule medicine is uniformly distributed in the liquid supply chamber 52.

[0035] In another embodiment, the upper cover 40 is further provided with a liquid inlet channel 42 and a liquid outlet channel 43 above the liquid storage cup 51; in this embodiment, both the liquid inlet channel and the liquid outlet channel extend into the liquid in the liquid supply chamber 52; the liquid inlet channel 42 allows the experimenter to conveniently add reagents or samples to the liquid supply chamber 52, while the liquid outlet channel 43 facilitates sampling or liquid discharge; both the liquid inlet channel 42 and the liquid outlet channel 43 are in communication with the liquid supply chamber 52 in the liquid storage cup 51; the inflow and outflow rates of the liquid can be controlled, which helps to achieve precise experimental operations; continuous flow analysis can be achieved through the liquid inlet channel and the liquid outlet channel, which is suitable for experiments that require continuous or semi-continuous operations and is flexible to use.

[0036] Alternatively, the liquid inlet channel 42 and the liquid outlet channel 43 are located on the two sides of the stirring assembly 41 respectively; the lower end of the liquid inlet channel 42 is lower in height than the lower end of the liquid outlet channel 43; alternatively, the size and position of the liquid inlet and outlet channels can be adjusted according to the use conditions to adapt to different volumes and types of experimental requirements, thereby improving the versatility of the device.

[0037] Further, the cartridge 30 is further provided with a sealed cavity 34 on one side of the accommodation cavity; the sealed cavity 34 is provided with a discharge assembly 35 for electrically conducting the electrolyte solution in the first compartment 32 and the second compartment 33; the discharge assembly 35 in this embodiment is a prior art, such as the discharge device 5 mentioned in the background art, which will not be described here; by controlling the electrical conduction of the electrolyte solution in the two compartments through the discharge assembly 35, electrochemical detection or electrolysis operation can be facilitated; by isolating the discharge assembly 35 from the electrolyte solution in the accommodation cavity, the discharge assembly is protected from direct contact with the solution, thereby prolonging the service life of the discharge assembly.

[0038] Further, the outer edge of the upper surface of the mounting plate 50 is circumferentially provided with an upwardly protruding positioning ring 54; the lower surface of the upper cover 40 is provided with a positioning groove (not shown in the figure) corresponding to and cooperatively inserted with the positioning ring 54; the positioning is accurate and can ensure that the stirring assembly on the upper cover is accurately inserted into the liquid supply chamber 52 in the cartridge.

[0039] Alternatively, the volume of the first compartment 32 is not less than the volume of the second compartment 33.

[0040] It should be understood that those skilled in the art can make improvements or changes based on the above description, and all such improvements and changes shall fall within the scope of protection of the appended claims of the present application.

Claims

1. A detection kit for detecting small molecule drugs, comprising a box body with an opening at the top and a top cover detachably connected to the box body; characterized in that... The upper end of the box body is provided with a mounting plate for opening or closing the opening; the box body and the mounting plate together form a receiving cavity; an insulating module is provided on the box body within the receiving cavity; the insulating module divides the receiving cavity into a first compartment and a second compartment; a liquid storage cup is provided in the middle of the area of ​​the first compartment on the mounting plate, which is recessed downward and extends towards the bottom of the box body; a liquid supply chamber is provided inside the liquid storage cup; a through hole is also provided on the side wall of the liquid storage cup to connect the liquid supply chamber and the first compartment, and a dialysis membrane is fixedly covered on the through hole; a stirring assembly is provided on the lower surface of the upper cover, which corresponds to the liquid storage cup and can stir the liquid in the liquid supply chamber.

2. The detection box according to claim 1, characterized in that, The inner walls of the opposite sides of the box are provided with guide grooves; the insulation module includes a support plate that engages with the two guide grooves and a sealing element that is tightly disposed between the outer edge of the support plate and the guide groove; an insulating diaphragm is fixedly disposed in the middle of the mounting plate to separate the electrolyte solution in the first compartment and the second compartment, and to allow ions in the first compartment to pass through into the second compartment.

3. The detection box according to claim 2, characterized in that, The insulating film is provided in multiple strips; each of the multiple insulating films is long and vertically arranged along the length of the mounting plate.

4. The detection box according to claim 3, characterized in that, The through-hole and the dialysis membrane are both located on the side wall of the reservoir.

5. The detection box according to any one of claims 1-4, characterized in that, The stirring assembly includes a stirring rod rotatably connected to the upper cover via a flange bearing, and a rotary motor that drives the stirring rod to rotate; the outer surface of the stirring rod is provided with multiple stirring blades along its length circumferentially.

6. The detection box according to claim 1, characterized in that, The upper cover is provided with an inlet channel and an outlet channel above the liquid storage cup; both the inlet channel and the outlet channel extend into the liquid in the supply chamber; both the inlet channel and the outlet channel are connected to the supply chamber inside the liquid storage cup.

7. The detection box according to claim 6, characterized in that, The inlet channel and the outlet channel are located on opposite sides of the stirring assembly; the lower end of the inlet channel is lower than the lower end of the outlet channel.

8. The detection box according to claim 1, characterized in that, The box body is further provided with a sealed cavity on one side of the receiving cavity; a discharge component for electrically conducting electrolyte solutions in the first compartment and the second compartment is sealed in the sealed cavity.

9. The detection box according to claim 1, characterized in that, The outer edge of the upper surface of the mounting plate is provided with an upwardly protruding positioning ring; the lower surface of the upper cover is provided with a positioning groove that corresponds to and cooperates with the positioning ring for positioning.

10. The detection box according to claim 9, characterized in that, The volume of the first compartment is not less than the volume of the second compartment.

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