Cell-free reactor for rapidly screening membrane protein
The cell-free reactor, which integrates a detergent module, a screening reaction module, and a result observation module, solves the problems of complex operation and unstable results in traditional membrane protein screening methods, realizes automation and high efficiency in membrane protein screening, and improves the accuracy and throughput of screening.
Patent Information
- Application Number
- CN202423230466.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-26
- Publication Date
- 2026-01-06
- Estimated Expiration
- 2034-12-26
AI Technical Summary
Traditional membrane protein screening methods are complex, costly, and easily affected by cell growth status and culture environment, making it difficult to guarantee the stability and accuracy of screening results.
Design a cell-free reactor that integrates a detergent module, a screening reaction module, and a result observation module to achieve automated and simultaneous screening of multiple membrane protein detergents. The reaction placement tray is driven by a mechanical module, which simplifies the operation process and improves the screening accuracy.
It has achieved automation, efficiency and precision in membrane protein screening, reduced operational difficulty, shortened screening cycle, improved screening throughput and result reliability, and avoided cross-contamination.
Smart Images
Figure CN223766307U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the technical field of reactors, and in particular to a cell-free reactor for rapid screening of membrane proteins. Background Technology
[0002] Membrane proteins are embedded in the cell's biological membrane and play a crucial role as the main executors of membrane functions. Structurally, membrane proteins can be broadly classified into external membrane proteins, internal membrane proteins, and lipid-anchored proteins. In the biomedical field, membrane proteins have extremely wide applications. The occurrence of many diseases is closely related to abnormal membrane protein function, making membrane proteins a core target for drug development. For example, in cancer treatment, certain membrane protein receptors on the surface of cancer cells are overexpressed or mutated. Monoclonal antibody drugs designed targeting these abnormal membrane proteins can precisely bind to cancer cells, blocking their growth signaling pathways or inducing an immune response to eliminate cancer cells. Membrane proteins also play a key role in drug transport. Some membrane transport proteins can affect drug absorption, distribution, metabolism, and excretion. By gaining a deeper understanding of the characteristics of these membrane transport proteins, researchers can optimize drug formulations and routes of administration, improving drug efficacy and reducing side effects. Furthermore, membrane proteins are widely used in the construction of drug screening models. Based on the interaction principle between membrane proteins and specific ligands, by establishing a high-throughput screening technology platform, potential drug molecules can be screened quickly and efficiently, which greatly accelerates the process of new drug development, brings new hope and light to the fight against various intractable diseases, and ensures that membrane proteins always remain at the forefront of the development of biomedical innovation.
[0003] Detergent screening plays a crucial role in membrane protein expression. Different membrane proteins require different detergents. Suitable detergents not only help stabilize membrane proteins and maintain their active conformation, but also increase their expression levels, optimize crystallization conditions, remove impurities, and reduce non-specific binding.
[0004] However, the research and drug development of membrane proteins face numerous challenges. The structural and functional properties of membrane proteins cause them to exhibit varying stability and activity in different detergent environments; therefore, selecting appropriate detergents is crucial for the research and drug development of membrane proteins.
[0005] Detergents play a crucial role in the expression of membrane proteins. They can disrupt cell membrane structure, releasing membrane proteins from the membrane and maintaining their stability and active conformation. Different membrane proteins exhibit varying selectivity for detergents. Appropriate detergents can not only enhance the expression levels of membrane proteins but also optimize their crystallization conditions, remove impurities, and reduce non-specific binding, thereby facilitating the structural analysis and functional study of membrane proteins.
[0006] Traditional membrane protein screening methods require the use of different instruments and detergents for individual testing, relying on a large amount of laboratory equipment and being quite cumbersome. This is not only complex and costly, but also easily affected by various factors such as cell growth status and culture environment, making it difficult to guarantee the stability and accuracy of screening results. Summary of the Invention
[0007] To address the shortcomings of existing technologies, the purpose of this application is to provide a cell-free reactor for rapid screening of membrane proteins, which can integrate various operations and quickly complete the screening.
[0008] The above-mentioned objective of this application is achieved through the following technical solution:
[0009] A cell-free reactor for rapid screening of membrane proteins, characterized in that it comprises a detergent module, a screening reaction module, a result observation module, and an operation module; the detergent module is equipped with a detergent storage tray and a detergent dispensing tray, the detergent storage tray and the detergent dispensing tray being detachably connected, the detergent storage tray having multiple independent detergent storage spaces, and the detergent dispensing tray having multiple detergent dispensing spoons, each of the detergent dispensing spoons being able to extend into the detergent storage space; the screening reaction module is equipped with a reaction placement tray and a screening reaction tray, the screening... A reaction plate is mounted on the reaction placement plate, forming an integral space within the screening reaction plate. A detergent dispensing plate is detachably mounted on the screening reaction plate, and the detergent dispensing plate divides the integral space into multiple reaction spaces, each corresponding to a detergent dispensing spoon. The result observation module is equipped with an observation placement plate, on which the screening reaction plate and the detergent dispensing plate can be mounted. The operation module includes a switch and a mechanical module, the mechanical module driving the reaction placement plate to move, and the switch controlling the start and stop of the mechanical module.
[0010] This application further provides that the screening reaction module is also provided with a reaction heating plate, which is located on one side of the reaction placement tray.
[0011] This application further specifies that the switch includes a heating switch for controlling the start and stop of the reaction heating element, and the heating switch is connected to the reaction heating element.
[0012] This application further specifies that the cell-free reactor for rapid screening of membrane proteins also includes an instrument cover, which covers the detergent module, the screening reaction module and the result observation module. The operation module is located outside the instrument cover, and a filter is provided on the instrument cover, which is positioned directly opposite the result observation module.
[0013] This application further specifies that the instrument cover has a partition that separates the detergent module, the screening reaction module, and the result observation module.
[0014] This application further specifies that the mechanical module includes a vibration limiting groove, a linkage rod, a motor, and a motor disk. The motor disk is connected to the motor, and the motor drives the motor disk to rotate. One end of the linkage rod is connected to the motor disk, and the other end of the linkage rod is connected to the reaction placement disk. The vibration limiting groove is located below the reaction placement disk, and a slider is also provided on the other end of the linkage rod. The slider is slidably disposed in the vibration limiting groove.
[0015] This application further specifies that the switch also includes a vibration switch for controlling the start and stop of the motor, and the vibration switch is connected to the motor.
[0016] This application further specifies that the result observation module is equipped with an observation light, and the switch also includes an observation switch for controlling the start and stop of the observation light, the observation switch being connected to the observation light.
[0017] This application is further configured such that the detergent dispensing tray and the detergent storage tray, as well as the detergent dispensing tray and the screening reaction tray, are connected by riveting.
[0018] This application is further configured such that both the detergent storage space and the reaction space are provided with grooves that fit the detergent dispensing spoon.
[0019] In summary, the beneficial technical effects of this application are as follows:
[0020] 1. This application can simultaneously screen detergent components of multiple membrane proteins, reducing system preparation operations and lowering operational difficulty.
[0021] 2. This application integrates a detergent module, a screening reaction module, and a result observation module, that is, it integrates the part of reaction and observation of membrane protein expression, achieving the effect of quickly completing membrane protein screening and directly observing the screening results.
[0022] 3. This application achieves automation, efficiency, and precision in the membrane protein screening process by integrating a detergent module, a screening reaction module, a result observation module, and an operation module. The reactor drives the reaction placement disk to move through a mechanical module, enabling simultaneous operation of multiple reaction spaces and significantly improving the screening throughput. At the same time, the design of the detergent module makes the use and replacement of detergent simple and quick, further shortening the screening cycle.
[0023] 4. This application provides multiple independent detergent storage spaces and corresponding scoops, ensuring that the most suitable detergent can be used in each reaction space, thereby improving the accuracy of screening. In addition, the design of the screening reaction plate makes each reaction space independent of each other, avoiding cross-contamination and further ensuring the reliability of the screening results.
[0024] 5. The detachable design of the detergent storage tray and dispensing tray in this application allows the detergent to be easily replaced and reused, avoiding waste. Attached Figure Description
[0025] Figure 1 This is a schematic diagram of a cell-free reactor used for rapid screening of membrane proteins.
[0026] Figure 2 This is a schematic diagram of the various modules of the reactor.
[0027] Figure 3 This is a schematic diagram showing the riveting method between the detergent storage tray and the detergent dispensing tray.
[0028] Figure 4 This is a schematic diagram showing the connection between the detergent storage tray and the detergent dispensing tray.
[0029] Figure 5 This is a schematic diagram showing the installation of the detergent storage tray and the detergent dispensing tray.
[0030] Figure 6 This is a schematic diagram showing the installation of the detergent dispensing tray, the screening reaction tray, and the reaction placement tray.
[0031] Figure 7 This is a schematic diagram showing the installation of the detergent dispensing tray, the screening reaction tray, and the observation tray.
[0032] Figure 8 This is a schematic diagram of the instrument cover and filter.
[0033] Figure 9 This is a schematic diagram of the instrument with the partition on.
[0034] Figure 10 This is a schematic diagram of a mechanical module.
[0035] Figure 11 This is a schematic diagram of the four states during the mechanical module drive process.
[0036] Figure 12 This is a schematic diagram showing the three states of the screening reaction module: setting the screening reaction plate, setting the screening reaction plate and the reaction placement plate, and setting the screening reaction plate, the reaction placement plate and the detergent dispensing plate.
[0037] Reference numerals: 10. Descaling agent module; 1001. Descaling agent storage tray; 1002. Descaling agent dispensing tray; 11. Screening reaction module; 1101. Screening reaction tray; 1102. Reaction placement tray; 1103. Reaction heating element; 12. Result observation module; 1201. Observation placement tray; 1203. Filter; 13. Operation module; 1301. Heating switch; 1302. Vibration switch; 1303. Observation switch; 14. Mechanical module; 1401. Vibration limiting groove; 1402. Linkage rod; 1403. Motor; 1404. Motor plate; 15. Instrument cover; 1501. Partition; O. Groove; S. Descaling agent dispensing spoon. Detailed Implementation
[0038] The present application will be further described in detail below with reference to the accompanying drawings.
[0039] like Figures 1-12 As shown, a cell-free reactor for rapid screening of membrane proteins is characterized by comprising a detergent module 10, a screening reaction module 11, a result observation module 12, and an operation module 13.
[0040] The detergent module 10 is equipped with a detergent storage tray 1001 and a detergent dispensing tray 1002, which are detachably connected. The detergent storage tray 1001 has multiple independent detergent storage spaces, and the detergent dispensing tray 1002 has multiple detergent dispensing spoons S, each of which can be inserted into a detergent storage space.
[0041] The screening reaction module 11 is provided with a reaction placement tray 1102 and a screening reaction tray 1101. The screening reaction tray 1101 is installed on the reaction placement tray 1102, and a whole space is formed inside the screening reaction tray 1101. The detergent dispensing tray 1002 is detachably installed on the screening reaction tray 1101. The detergent dispensing tray 1002 divides the whole space to form multiple reaction spaces, and each reaction space corresponds to a detergent dispensing spoon S.
[0042] The result observation module 12 is equipped with an observation placement plate 1201, and the screening reaction plate 1101 and the detergent dispensing plate 1002 can be installed on the observation placement plate 1201.
[0043] The operation module 13 includes a switch and a mechanical module 14. The mechanical module 14 drives the reaction placement disk 1102 to move, and the switch controls the start and stop of the mechanical module 14.
[0044] like Figures 1-2 As shown, this application integrates multiple structures for the storage, use, cell-free reaction, and observation of screening results of the detergent.
[0045] like Figure 5 As shown, the detergent module 10 includes a detergent storage tray 1001 and a detergent dispensing tray 1002. The detergent storage tray 1001 stores ten detergents to be screened. The detergent dispensing tray 1002 is attached to the storage tray; after quantitatively dispensing the detergent, it is added to the subsequent screening reaction tray 1101. Figures 3-4 As shown, a special riveting structure is used between the detergent dispensing tray 1002 and the detergent storage tray 1001 to facilitate the retrieval of the last liquid in the storage tray. Specifically, the detergent dispensing tray 1002 is formed into a recess, and the detergent storage tray 1001 is formed into a protrusion. The protrusion and the recess complement each other and support each other, which is the riveting structure.
[0046] like Figure 2 , Figure 6 and Figure 12 As shown, the screening reaction module 11 includes a screening reaction disk 1101, a reaction placement disk 1102, and a reaction heating plate 1103. The screening reaction disk 1101, initially connected in its reaction space, is divided into ten independent reaction zones after the descaling agent dispensing disk 1002 is loaded, allowing for cell-free reactions with ten different descaling agent systems. The screening reactor is made of a transparent material to facilitate subsequent observation and reading of fluorescence signals. The reaction placement disk 1102 is a metal disc with a designated area for placing the screening reaction disk 1101. The metal disk also facilitates the transfer of heat supplied by the reaction heating plate 1103 to the screening reaction disk 1101. The reaction placement disk 1102 is connected to the linkage rod 1402 of the mechanical module 14, as shown below. Figures 10-11 As shown, it can be driven to form a reciprocating motion, providing the oscillating conditions required for cell-free protein expression reactions. The reaction heating element 1103 provides the constant temperature conditions required for cell-free reactions.
[0047] The result observation module 12 includes an observation placement tray 1201, an observation lamp, and a filter 1203. The observation placement tray 1201 has a limiting groove for placing the screening reaction tray 1101, which helps to place the screening reaction tray 1101 in the correct spatial position. The observation placement tray 1201 is made of transparent material to facilitate the observation of the reaction fluorescence signal. Below the observation placement tray 1201 is the observation lamp, which is a blue LED that provides excitation energy for the green fluorescence produced by the reaction. The instrument cover 15 in the observation module area has a filter 1203, which is orange-yellow. Its function is to filter out the blue excitation light while allowing the green fluorescence of the reaction signal to pass through, thereby reducing interference with the observation and harm to the observer's eyes.
[0048] The operation module 13 includes a heating switch 1301, a vibration switch 1302, and an observation switch 1303. The heating switch 1301 controls the heating state during the cell-free protein expression reaction. The vibration switch 1302 controls the oscillation state during the cell-free protein expression reaction. The observation switch 1303 controls the state of the excitation light during the final result observation.
[0049] like Figures 10-11 As shown, the mechanical module 14 includes a vibration limiting groove 1401, a linkage rod 1402, a motor 1403, and a motor disk 1404, which provides power for the oscillation state required for the reaction. The vibration limiting groove 1401 is a component that restricts the movement of the reaction placement disk 1102 at the bottom, causing the reaction placement disk 1102 to move in a regular linear reciprocating motion. The linkage rod 1402 is a connecting component that transmits the circular motion of the motor 1403 to the reaction placement disk 1102. The motor 1403 provides power for the reciprocating motion. The motor disk 1404 amplifies the rotation of the motor 1403 and connects to the transmission rod to realize the transmission of motion.
[0050] like Figures 8-9 As shown, the instrument cover 15 is equipped with a partition 1501 to separate and seal the reaction space, reducing internal and external interference.
[0051] It is worth noting that, see Figures 3-4 As shown, a groove O is provided at the bottom of the detergent storage space. The groove O is the lowest point of the detergent storage space, which ensures that the detergent will definitely stay in the groove O. The detergent scoop S can be inserted into the groove O. The shape of the detergent scoop S matches the shape of the groove O, thus ensuring that the detergent scoop S can obtain the detergent in the detergent storage space.
[0052] The procedure for using this device is as follows: Add the detergents numbered I to X into the respective detergent storage spaces of the detergent storage tray 1001, ready for use. It is worth noting that after the reaction is complete, the device can be covered and stored directly at 4°C for future use.
[0053] Liquids A and B are mixed in a predetermined ratio and set aside for later use. The screening reaction tray 1101 is placed on the limiting groove of the reaction placement tray 1102, and the mixed reaction solution of A and B is added to it. Before the descaling agent dispensing tray 1002 is loaded onto the screening reaction tray 1101, the reaction space is in a connected state. When adding the mixed reaction solution of A and B, it is not necessary to add it separately; it only needs to be added once, simplifying the operation. The descaling agent dispensing tray 1002 is loaded onto the descaling agent storage tray 1001. The descaling agent dispensing spoons S on the multiple descaling agent dispensing trays 1002 will pick up different descaling agents from the descaling agent storage space I to X. Then, the descaling agent dispensing tray 1002 is removed and loaded onto the screening reaction tray 1101. The overall space on the screening reaction tray 1101 is divided into ten independent areas by the detergent dispensing tray 1002. Specifically, the detergent dispensing tray 1002 is equipped with a partition plate 1501, which divides the overall space into reaction spaces. Each reaction space is independent and complementary to the others. Each detergent dispensing spoon S is inserted into one reaction space, and each reaction space corresponds to one detergent, thus achieving the screening of ten different detergent reaction systems.
[0054] After loading, close the instrument cover 15 to reduce external interference with the internal reaction. Turn on the heating switch 1301 and vibration switch 1302 to activate the reaction heating element 1103 and motor 1403, providing a constant-temperature, oscillating environment for the cell-free protein expression reaction. After several 6-hour cycles, turn off the heating switch 1301 and vibration switch 1302 to terminate the reaction. Remove the instrument cover 15 for subsequent operations.
[0055] Remove the screening reaction tray 1101 and the detergent dispensing tray 1002 simultaneously from the reaction placement tray 1102 and place them on the limiting groove of the observation placement tray 1201. Cover the instrument with the instrument cover 15 and turn on the observation switch 1303. The observation light will illuminate. Observe and record the reaction results through the filter 1203 on the instrument cover 15. Turn off the observation switch 1303, remove the screening reaction tray 1101 and the detergent dispensing tray 1002, and clean them. Cover the instrument with the instrument cover 15 and store the entire set at 4°C for future use.
[0056] This application simplifies the liquid addition operation after system configuration, streamlining the overall process. It standardizes the screening process, lowers the barrier to entry for users, and makes it easier to learn. The reactor uses fluorescence as a signal, making the final results easy and direct to observe.
[0057] Specifically, one embodiment of this application is as follows.
[0058] Prepare ten detergents: Brij35, DDM, Digitonin, Brij58, Brij78, TritonX-100, LMNG, GDN, GenapolX-80, and TWEEN20.
[0059] According to the instructions, use the components and reagents required for the cell-free protein expression reaction.
[0060] The mixture consists of two reagents, A and B. Reagent A contains 57 mM HEPES-KOH buffer, 1.5 mM ATP, 1.5 mM UTP, 1.5 mM CTP, 1.5 mM GTP, 0.64 mM cAMP, 100 mM potassium glutamate, 12 mM ammonium acetate, 15 mM magnesium acetate, 2 mM amino acids, 2% PEG8000 (by weight), 33 mM phosphoenolpyruvate, and 25% E. coli extract (by volume). Reagent B is a membrane protein fusion GFP plasmid.
[0061] Where mM stands for millimoles per liter.
[0062] Add the detergents numbered I to X to the detergent storage tray 1001, ready for use. It is worth noting that I to X refer to the ten Roman characters I, II, III, IV, V, VI, VII, VIII, IX, and X.
[0063] After the reaction is complete, the device can be covered and placed directly at 4°C to store the detergent for future use.
[0064] The screening reaction tray 1101 is placed on the limiting groove of the reaction placement tray 1102, and the mixed reaction solution of A and B is added to it. Before the descaling agent dispensing tray 1002 is loaded onto the screening reaction tray 1101, the reaction space is in a connected state. When adding the mixed reaction solution of A and B, it is not necessary to add it separately, but only once, simplifying the operation. The descaling agent dispensing tray 1002 is loaded onto the descaling agent storage tray 1001, and the descaling agent dispensing spoons S on the multiple descaling agent dispensing trays 1002 will pick up different descaling agents from the descaling agent storage space I to X. Then the descaling agent dispensing tray 1002 is removed and loaded onto the screening reaction tray 1101. The overall space on the screening reaction tray 1101 is divided into ten independent areas by the descaling agent dispensing tray 1002. Specifically, the descaling agent dispensing tray 1002 is provided with a partition plate 1501, which divides the overall space into reaction spaces. Each reaction space is independent and complementary to each other. Furthermore, each detergent scoop S is inserted into a reaction space, and each reaction space corresponds to a detergent, thus achieving the screening of ten different detergent reaction systems.
[0065] After loading, close the instrument cover 15 to reduce external interference with the internal reaction. Turn on the heating switch 1301 and vibration switch 1302 to activate the reaction heating element 1103 and motor 1403, providing a constant-temperature, oscillating environment for the cell-free protein expression reaction. After 6 hours of reaction, turn off the heating switch 1301 and vibration switch 1302 to terminate the reaction. Remove the instrument cover 15 for subsequent operations.
[0066] Remove the screening reaction tray 1101 and the detergent dispensing tray 1002 simultaneously from the reaction placement tray 1102 and place them on the limiting groove of the observation placement tray 1201. Cover the instrument with the instrument cover 15 and turn on the observation switch 1303. The observation light will illuminate. Observe and record the reaction results through the filter 1203 on the instrument cover 15. Turn off the observation switch 1303, remove the screening reaction tray 1101 and the detergent dispensing tray 1002, and clean them. Cover the instrument with the instrument cover 15 and place the entire instrument at 4°C for the next use.
[0067] It is also worth noting that for membrane proteins expressed in traditional cellular methods, screening requires prior expression and purification of the membrane proteins before the appropriate screening tests can be performed. This process is relatively complex and carries a high risk of failure.
[0068] This application, by incorporating a cell-free protein synthesis (CFPS) system, simplifies the steps of membrane protein expression and purification. The CFPS system is an in vitro gene expression system that uses exogenous DNA or mRNA as a template, artificially adds necessary raw materials and energy substances, and uses cell extracts as a condition to synthesize proteins. It can overcome cellular limitations and conveniently and rapidly express various proteins. Specifically, CFPS uses DNA as a template, and under the action of RNA polymerase and transcription factors, transcribes the corresponding mRNA; using mRNA as a template, it translates and synthesizes proteins using ribosomes, amino acid substrates, tRNA, and energy substances within the system.
[0069] This application leverages the open nature of cell-free systems by adding different detergents to the system during protein expression, allowing the protein to come into contact with the detergent during the expression process. This effectively avoids the problem of difficult expression of membrane proteins within cells and reduces the number of purification steps after protein expression. It also enables rapid identification of suitable detergents for specific membrane proteins based on fluorescence signals after protein expression.
[0070] The embodiments described herein are preferred embodiments of this utility model and are not intended to limit the scope of protection of this utility model. Therefore, all equivalent changes made to the structure, shape, and principle of this utility model should be included within the scope of protection of this utility model.
Claims
1. A cell-free reactor for rapid screening of membrane proteins, characterized in that, The device comprises a detergent module (10), a screening reaction module (11), a result observation module (12) and an operation module (13). The detergent module (10) is provided with a detergent storage disc (1001) and a detergent taking disc (1002), the detergent storage disc (1001) and the detergent taking disc (1002) are detachably connected, the detergent storage disc (1001) has a plurality of independent detergent storage spaces, and the detergent taking disc (1002) has a plurality of detergent taking spoons (S), each of which can extend into the detergent storage space. The screening reaction module (11) is provided with a reaction placement disc (1102) and a screening reaction disc (1101), the screening reaction disc (1101) is installed on the reaction placement disc (1102), the screening reaction disc (1101) forms an integral space, the detergent taking disc (1002) is detachably installed on the screening reaction disc (1101), the detergent taking disc (1002) divides the integral space into a plurality of reaction spaces, and each reaction space corresponds to a detergent taking spoon (S). The result observation module (12) is provided with an observation placement disc (1201), and the screening reaction disc (1101) and the detergent taking disc (1002) can be installed on the observation placement disc (1201). The operation module (13) comprises a switch and a mechanical module (14), the mechanical module (14) drives the reaction placement disc (1102) to move, and the switch controls the start and stop of the mechanical module (14).
2. The cell-free reactor for rapid screening of membrane proteins according to claim 1, wherein, The screening reaction module (11) is further provided with a reaction heating sheet (1103), and the reaction heating sheet (1103) is arranged on one side of the reaction placement disc (1102).
3. The cell-free reactor for rapid screening of membrane proteins according to claim 2, wherein, The switch comprises a heating switch (1301) for controlling the start and stop of the reaction heating sheet (1103), and the heating switch (1301) is connected with the reaction heating sheet (1103).
4. The cell-free reactor for rapid screening of membrane proteins according to claim 1, wherein, The device further comprises an instrument cover (15), the instrument cover (15) covers the detergent module (10), the screening reaction module (11) and the result observation module (12), the operation module (13) is arranged outside the instrument cover (15), the instrument cover (15) is provided with a filter (1203), and the filter (1203) is arranged opposite to the result observation module (12).
5. The cell-free reactor for rapid screening of membrane proteins according to claim 4, wherein, The instrument cover (15) is provided with a partition plate (1501), and the partition plate (1501) separates the detergent module (10), the screening reaction module (11) and the result observation module (12).
6. The cell-free reactor for rapid screening of membrane proteins according to claim 1, wherein, The mechanical module (14) comprises a vibration limiting groove (1401), a linkage rod (1402), a motor (1403) and a motor disc (1404), the motor disc (1404) is connected with the motor (1403), the motor (1403) drives the motor disc (1404) to rotate, one end of the linkage rod (1402) is connected on the motor disc (1404), the other end of the linkage rod (1402) is connected with the reaction placing disc (1102), the vibration limiting groove (1401) is arranged below the reaction placing disc (1102), and a sliding block is further arranged on the other end of the linkage rod (1402), and the sliding block is slidably arranged in the vibration limiting groove (1401).
7. The cell-free reactor for rapid screening of membrane proteins according to claim 6, wherein, The switch further comprises a vibration switch (1302) for controlling the start and stop of the motor (1403), and the vibration switch (1302) is connected with the motor (1403).
8. The cell-free reactor for rapid screening of membrane proteins according to claim 1, wherein, The result observation module (12) is provided with an observation lamp, and the switch further comprises an observation switch (1303) for controlling the start and stop of the observation lamp, and the observation switch (1303) is connected with the observation lamp.
9. The cell-free reactor for rapid screening of membrane proteins according to claim 1, wherein, The detergent taking disc (1002) and the detergent storage disc (1001) and the detergent taking disc (1002) and the screening reaction disc (1101) are connected in a riveting manner.
10. The cell-free reactor for rapid screening of membrane proteins according to claim 9, wherein, The detergent storage space and the reaction space are both provided with a groove (O) matched with the detergent taking spoon (S).