Fluorescent PCR (Polymerase Chain Reaction) detection quantitative analyzer

Abstract

The utility model relates to the technical field of fluorescent PCR (Polymerase Chain Reaction) detection, and discloses a quantitative analyzer for fluorescent PCR detection, which comprises a working platform, a power box, a support base, an analysis platform, a fixed frame and a fluorescent PCR detection plate, a supporting base is fixedly arranged at the lower end of the power box, an analysis platform is fixedly arranged at the upper end of the working platform, a fixed frame is fixedly arranged at the upper end of the analysis platform, and a fluorescent PCR detection plate is fixedly arranged at the upper end of the fixed frame. According to the fluorescent PCR detection quantitative analyzer, the quantitative analyzer is improved, so that the analysis heat dissipation capability of the quantitative analyzer is improved, in the actual use process of the quantitative analyzer, PCR fluorescence detection results can be rapidly and conveniently analyzed according to needs, heat dissipation use of the PCR fluorescence analyzer is facilitated, and the quantitative analyzer is convenient to use. And the analysis effect of the fluorescent PCR detection quantitative analyzer is improved.

Description

Technical Field

[0001] This utility model relates to the field of fluorescent PCR detection technology, specifically to a fluorescent PCR detection and quantitative analyzer. Background Technology

[0002] Fluorescent PCR is a method that uses fluorescent chemicals to measure the total amount of product after each polymerase chain reaction (PCR) cycle in a DNA amplification reaction. It is a method for quantitative analysis of specific DNA sequences in the sample to be tested using internal or external reference methods.

[0003] The prior art patent document CN210215375U discloses a PCR fluorescence detector. The support is cross-shaped and includes a first rod and a second rod arranged perpendicularly to each other. A PCR reaction chamber is fixed to each end of the first and second rods. A through hole is provided at the connection point of the first and second rods. A support rod is fixed at the center of a circular substrate and is rotatably inserted into the through hole. The PCR fluorescence detector also includes a first heating device, a second heating device, a cooling device, and a detection device located below the circular substrate. The first heating device corresponds to the high-temperature heating denaturation and lysis zone, the second heating device corresponds to the low-temperature polymerization renaturation and extension zone, the cooling device corresponds to the rapid cooling zone, and the detection device corresponds to the fluorescence detection zone. The PCR fluorescence detector also includes a lifting device located below the circular substrate, to which the first heating device is fixed. The distance between the first heating device and the circular substrate is adjusted by the lifting device. The temperature of the high-temperature heating denaturation and lysis zone is 90-100°C, the temperature of the low-temperature polymerization renaturation and extension zone is 60-70°C, and the temperature of the rapid cooling zone is 10-50°C. The surfaces of the high-temperature heating denaturation and lysis zone, the rapid cooling zone, and the low-temperature polymerization renaturation and extension zone facing the PCR reaction chamber are smooth metal surfaces. The PCR fluorescence detector is equipped with multiple independent functional zones, and the PCR reaction chamber completes the lysis, amplification, and fluorescence detection in different functional zones by rotation. This avoids the heating and cooling process at the same point, shortening the detection time to 20-30 minutes and greatly improving the detection speed. In addition, the heating device of the high-temperature heating denaturation and lysis zone is equipped with a lifting device to control its height, which allows the heating device to quickly detach after reaching the set heating time, ensuring that the PCR reaction chamber in the long low-temperature polymerization renaturation and extension zone has sufficient reaction time, resulting in more reliable detection results.

[0004] However, in the existing technology CN210215375U patent, the PCR fluorescence detector has poor heat dissipation capability, which makes it impossible to quickly and conveniently analyze the PCR fluorescence detection results as needed during actual use. This also hinders the heat dissipation of the PCR fluorescence detector and affects its analytical effect. Utility Model Content

[0005] (a) Technical problems to be solved

[0006] The purpose of this invention is to provide a fluorescence PCR detection and quantitative analyzer to solve the problem of poor heat dissipation in PCR fluorescence detectors mentioned in the background art.

[0007] (II) Technical Solution

[0008] To achieve the above objectives, this utility model provides the following technical solution: a fluorescence PCR detection and quantitative analyzer, which includes a working platform, a power supply box, a support base, an analysis platform, a fixed frame, a fluorescence PCR detection plate, a movable frame, movable protrusions, a detection tray, a connecting plate, a convenient handle, an analysis motor, an indicator light, a mounting plate, a fixed cylinder, a signal interaction device, a heat dissipation cylinder, a heat dissipation plate, a connecting rod, and heat dissipation fan blades. The power supply box is fixedly installed at the lower end of the working platform, and the support base is fixedly installed at the lower end of the power supply box. The analysis platform is fixedly installed at the upper end of the working platform, and the fixed frame is fixedly installed at the upper end of the analysis platform. The fluorescence PCR detection plate is fixedly installed at the upper end of the fixed frame, and the movable frame is fixedly installed at the left end of the analysis platform. Movable protrusions are movably installed inside the movable frame.

[0009] Preferably, a detection tray is movably arranged inside the movable frame, and a connecting plate is fixedly arranged at the left end of the detection tray. Through the improvement of the quantitative analyzer, the analysis and heat dissipation capacity of the quantitative analyzer is improved. In the actual use of the quantitative analyzer, the PCR fluorescence detection results can be analyzed quickly and conveniently as needed, which facilitates the heat dissipation of the PCR fluorescence detector and improves the analysis effect of the fluorescence PCR detection quantitative analyzer.

[0010] Preferably, a convenient handle is fixedly installed on the left end of the connecting plate, and an analysis motor is fixedly installed on the upper end of the analysis platform. The installed working platform, power supply box, and support base improve the battery life of the fluorescence PCR detection quantitative analyzer. The installed analysis platform, fixed frame, and fluorescence PCR detection plate facilitate the detection of the fluorescence PCR detection quantitative analyzer. The installation of the movable frame, movable protrusion, and detection tray facilitates the detection work of the fluorescence PCR detector. The installation of the connecting plate and convenient handle facilitates the opening and closing of the detection tray.

[0011] Preferably, an indicator light is fixedly installed at the upper end of the analytical motor, and a mounting plate is fixedly installed at the upper end of the analytical motor.

[0012] Preferably, a fixing cylinder is fixedly installed at the upper end of the mounting plate, and a signal interaction device is fixedly installed at the upper end of the fixing cylinder. By installing the analysis motor and signal light, the analysis effect of the fluorescence PCR detection quantitative analyzer is improved. Then, by installing the mounting plate, fixing cylinder and signal interaction device, the signal interaction capability of the fluorescence PCR detection quantitative analyzer is improved. Finally, by installing the heat sink, connecting rod and heat dissipation fan blades, the heat dissipation effect of the fluorescence PCR detection quantitative analyzer is improved.

[0013] Preferably, a heat sink is fixedly provided at the left end of the analysis motor, and a heat sink plate is fixedly provided at the left end of the heat sink.

[0014] Preferably, a connecting rod is fixedly installed inside the heat sink, and a heat dissipation fan blade is fixedly installed at the left end of the connecting rod.

[0015] Compared with the prior art, the beneficial effects of this utility model are:

[0016] 1. This fluorescence PCR detection and quantitative analyzer improves the heat dissipation capability of the analyzer through improvements. In actual use, the PCR fluorescence detection results can be analyzed quickly and conveniently as needed, which facilitates the heat dissipation of the PCR fluorescence detector and improves the analysis effect of the fluorescence PCR detection and quantitative analyzer.

[0017] 2. This fluorescent PCR detection and quantitative analyzer improves the battery life of the analyzer through the installation of the working platform, power supply box, and support base. The installed analysis platform, fixed frame, and fluorescent PCR detection plate facilitate the detection of the fluorescent PCR detection and quantitative analyzer. The installation of the movable frame, movable protrusion, and detection tray facilitates the detection work of the fluorescent PCR detector. The installation of the connecting plate and convenient handle facilitates the opening and closing of the detection tray.

[0018] 3. This fluorescence PCR detection and quantitative analyzer improves the analytical effect by installing the analysis motor and signal lights. Then, it improves the signal interaction capability by installing the mounting plate, fixing cylinder, and signal interaction device. Finally, it improves the heat dissipation effect by installing the heat sink, connecting rod, and heat dissipation fan blades. Attached Figure Description

[0019] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0020] Figure 2 This is a schematic diagram of the detection tray structure of this utility model;

[0021] Figure 3 This is a schematic diagram of the signal interaction device of this utility model;

[0022] Figure 4 This is a schematic diagram of the heat dissipation fan blade structure of this utility model.

[0023] In the diagram: 1. Working platform; 2. Power supply box; 3. Support base; 4. Analysis platform; 5. Fixed frame; 6. Fluorescent PCR detection plate; 7. Movable frame; 8. Movable protrusion; 9. Detection tray; 10. Connecting plate; 11. Handle; 12. Analysis motor; 13. Indicator light; 14. Mounting plate; 15. Fixing cylinder; 16. Signal interaction device; 17. Heat sink; 18. Heat sink plate; 19. Connecting rod; 20. Heat sink fan blades. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0025] Please see Figures 1-4This utility model provides a technical solution: a fluorescence PCR detection and quantitative analyzer, which includes a working platform 1, a power supply box 2, a support base 3, an analysis platform 4, a fixed frame 5, a fluorescence PCR detection plate 6, a movable frame 7, a movable protrusion 8, a detection tray 9, a connecting plate 10, a convenient handle 11, an analysis motor 12, an indicator light 13, a mounting plate 14, a fixed cylinder 15, a signal interaction device 16, a heat dissipation cylinder 17, a heat dissipation plate 18, a connecting rod 19, and a heat dissipation fan blade 20. The power supply box 2 is fixedly installed at the lower end of the working platform 1, and the support base 3 is fixedly installed at the lower end of the power supply box 2. The analysis platform 4 is fixedly installed at the upper end of the working platform 1, and the fixed frame 5 is fixedly installed at the upper end of the analysis platform 4. The fluorescence PCR detection plate 6 is fixedly installed at the upper end of the fixed frame 5, and the movable frame 7 is fixedly installed at the left end of the analysis platform 4. The movable protrusion 8 is movably installed inside the movable frame 7.

[0026] The movable frame 7 houses a detection tray 9, with a connecting plate 10 fixed to the left end of the detection tray 9 and a convenient handle 11 fixed to the left end of the connecting plate 10. An analysis motor 12 is fixed to the upper end of the analysis platform 4, with an indicator light 13 fixed to the upper end of the analysis motor 12 and a mounting plate 14 fixed to the upper end of the analysis motor 12. The installed work platform 1, power supply box 2, and support base 3 improve the battery life of the fluorescence PCR quantitative analyzer. The installed analysis platform 4, fixed frame 5, and fluorescence PCR detection plate 6 facilitate the detection process of the fluorescence PCR quantitative analyzer. The installation of the movable frame 7, movable protrusion 8, and detection tray 9 facilitates the detection work of the fluorescence PCR analyzer. The installation of the connecting plate 10 and convenient handle 11 facilitates the opening and closing of the detection tray 9.

[0027] A fixing cylinder 15 is fixedly installed on the upper end of the mounting plate 14, and a signal interaction device 16 is fixedly installed on the upper end of the fixing cylinder 15. A heat sink 17 is fixedly installed on the left end of the analysis motor 12, and a heat sink plate 18 is fixedly installed on the left end of the heat sink 17. A connecting rod 19 is fixedly installed inside the heat sink 17, and a heat dissipation fan blade 20 is fixedly installed on the left end of the connecting rod 19. By installing the analysis motor 12 and the signal light 13, the analysis effect of the fluorescence PCR detection quantitative analyzer is improved. Then, by installing the mounting plate 14, the fixing cylinder 15, and the signal interaction device 16, the signal interaction capability of the fluorescence PCR detection quantitative analyzer is improved. Finally, by installing the heat sink plate 18, the connecting rod 19, and the heat dissipation fan blade 20, the heat dissipation effect of the fluorescence PCR detection quantitative analyzer is improved.

[0028] Working Principle: The installed working platform 1, power supply box 2, and support base 3 improve the battery life of the fluorescence PCR quantitative analyzer. The installed analysis platform 4, fixed frame 5, and fluorescence PCR detection plate 6 facilitate the detection of the fluorescence PCR quantitative analyzer. The installation of the movable frame 7, movable protrusion 8, and detection tray 9 facilitates the detection operation of the fluorescence PCR detector. The installation of the connecting plate 10 and convenient handle 11 facilitates the opening and closing of the detection tray 9. The installation of the analysis motor 12 and indicator light 13 improves the analysis effect of the fluorescence PCR quantitative analyzer. The installation of the mounting plate 14, fixed cylinder 15, and signal interaction device 16 improves the signal interaction capability of the fluorescence PCR quantitative analyzer. The installation of the heat sink 18, connecting rod 19, and heat dissipation fan blades 20 improves the heat dissipation effect of the fluorescence PCR quantitative analyzer.

[0029] Finally, it should be noted that the above content is only used to illustrate the technical solution of this utility model, and is not intended to limit the scope of protection of this utility model. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of this utility model do not depart from the essence and scope of the technical solution of this utility model.

Claims

1. A fluorescent PCR detection quantitative analyzer, comprising a work platform (1), a power supply box (2), a supporting base (3) and an analysis platform (4), characterized in that: The lower end of the working platform (1) is fixedly provided with a power box (2), the lower end of the power box (2) is fixedly provided with a supporting base (3), the upper end of the working platform (1) is fixedly provided with an analysis platform (4), the upper end of the analysis platform (4) is fixedly provided with a fixed frame (5), the upper end of the fixed frame (5) is fixedly provided with a fluorescent PCR detection plate (6), the left end of the analysis platform (4) is fixedly provided with a movable frame (7), the inside of the movable frame (7) is movably provided with a movable lug (8), the inside of the movable frame (7) is movably provided with a detection supporting plate (9), the left end of the detection supporting plate (9) is fixedly provided with a connecting plate (10), the left end of the connecting plate (10) is fixedly provided with a convenient handle (11), the upper end of the analysis platform (4) is fixedly provided with an analysis motor (12), the upper end of the analysis motor (12) is fixedly provided with a signal lamp (13), the upper end of the analysis motor (12) is fixedly provided with a mounting plate (14), the upper end of the mounting plate (14) is fixedly provided with a fixed cylinder (15), the upper end of the fixed cylinder (15) is fixedly provided with a signal interaction device (16), the left end of the analysis motor (12) is fixedly provided with a heat dissipation cylinder (17), the left end of the heat dissipation cylinder (17) is fixedly provided with a heat dissipation plate (18), the inside of the heat dissipation cylinder (17) is fixedly provided with a connecting rod (19), and the left end of the connecting rod (19) is fixedly provided with a heat dissipation fan blade (20).

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