Dropper for high-sensitivity detection of tachypleus tridentatus recombinant factor C endotoxin
By introducing an overflow tube and a collection component into the dropper and using a guide plate structure to divert excess solution, the contamination problem in the state where the dropper and the cap are connected is solved, achieving efficient collection and purity maintenance of the solution and improving the reliability of the detection.
Patent Information
- Application Number
- CN202520334643.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2026-01-06
- Estimated Expiration
- 2035-02-28
AI Technical Summary
During the detection of factor C in horseshoe crabs, repeated squeezing or excessive selection of solution while the dropper and cap were connected caused the solution to enter the cap, resulting in contamination and reduced solution purity.
Design a dropper structure including a sampling tube, a cap, an overflow tube, and a collection assembly. Utilize the inclined distribution of the first and second guide plates to guide excess solution to the overflow tube and collect it in the collection tube, preventing solution from entering the cap.
This effectively prevents the solution from entering the cap, improving the practicality of the dropper and the purity of the solution, and ensuring the accuracy and reliability of the test.
Smart Images
Figure CN223769843U_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of biotoxin detection technology, specifically relating to a dropper for the highly sensitive detection of recombinant factor C endotoxin in horseshoe crabs. Background Technology
[0002] Recombinant Limulus Amebocyte Lysate (LAL) C factor is a genetically engineered protein used for highly sensitive endotoxin detection. Endotoxins are lipopolysaccharides (LPS) found in the cell walls of Gram-negative bacteria, possessing potent biological activity that can trigger severe inflammatory responses and other pathological effects. Traditional Limulus Amebocyte Lysate (LAL) assays rely on amoeboid cells extracted from horseshoe crab blood, while recombinant LAL C factor technology offers a more sustainable and environmentally friendly alternative through recombinant proteins.
[0003] In the process of detecting endotoxins using recombinant factor C from horseshoe crabs, multiple reagents need to be mixed for testing to facilitate toxin monitoring. Currently, most reagent selection in experiments uses droppers for collection. For example, when selecting an appropriate amount of sample to be tested, such as milk or beverage, the dropper and the cap are in a connected state. When using the dropper to select the solution, it is easy for solution to enter the cap due to repeated squeezing or excessive selection. This not only contaminates the cap but also reduces the purity of the solution. It is also inconvenient to export and collect the excess solution, thus reducing its practical impact. Utility Model Content
[0004] The purpose of this invention is to provide a dropper for the highly sensitive detection of recombinant factor C endotoxin in horseshoe crabs, which can export and collect excess solution when selecting solution with the dropper, effectively avoiding the phenomenon of solution entering the capsule.
[0005] The specific technical solution adopted in this utility model is as follows:
[0006] A dropper for detecting recombinant factor C endotoxin in horseshoe crabs includes a sampling tube and a cap. The cap is fitted to the top of the sampling tube. An overflow tube is fixedly connected to the side wall of the sampling tube. A collection assembly is fitted to the other end of the overflow tube. A one-way valve is fitted to the inner wall of the overflow tube. A first guide plate and a second guide plate are fixedly connected at the connection between the inner wall of the sampling tube and the overflow tube.
[0007] The collection assembly includes a collection tube assembled at one end of the overflow pipe, a sealing cap assembled at one end of the collection tube, baffles fixedly connected to the side walls of the overflow pipe and the collection tube respectively, and a rubber sleeve connected between the two baffles.
[0008] The first guide plate and the second guide plate are arranged in parallel with a downward tilt. The second guide plate is located above the first guide plate, and the end of the second guide plate near the overflow pipe is arc-shaped.
[0009] A shielding layer is fixedly connected to the inner wall of the sampling tube near the top.
[0010] A scale is provided on the side wall of the sampling tube.
[0011] The overflow pipe and the collection pipe are arranged at a downward angle.
[0012] The technical effects achieved by this utility model are as follows:
[0013] This utility model relates to a dropper for highly sensitive detection of recombinant factor C endotoxin in horseshoe crabs. Through the cooperation of a sampling tube, a cap, a first guide plate, a second guide plate, and an overflow tube, and by utilizing the distribution structure of the first and second guide plates, excess solution can be guided to the overflow tube during solution selection. This effectively avoids contamination caused by solution entering the cap, thereby significantly improving its practicality. Attached Figure Description
[0014] Figure 1 This is a perspective view of this utility model embodiment;
[0015] Figure 2 This is a cross-sectional structural schematic diagram of this utility model embodiment;
[0016] Figure 3 This is a perspective view of the components collected in this utility model embodiment;
[0017] Figure 4 This is a cross-sectional structural diagram of the collection component in this utility model embodiment.
[0018] The attached diagram lists the components represented by each number as follows:
[0019] 1. Sampling tube; 2. Rubber cap; 3. Overflow tube; 4. Collection tube; 5. One-way valve; 6. First guide plate; 7. Second guide plate; 8. Baffle; 9. Rubber sleeve; 10. Sealing cap; 11. Shielding layer; 12. Ruler. Detailed Implementation
[0020] To make the purpose and advantages of this utility model clearer, the following detailed description is provided in conjunction with embodiments. It should be understood that the following text is merely used to describe one or more specific implementations of this utility model and does not strictly limit the scope of protection specifically claimed by this utility model.
[0021] like Figures 1-4 As shown, a dropper for detecting recombinant factor C endotoxin in horseshoe crabs includes a sampling tube 1 and a cap 2. The cap 2 is assembled on the top of the sampling tube 1. An overflow tube 3 is fixedly connected to the side wall of the sampling tube 1. A collection assembly is assembled at the other end of the overflow tube 3. A one-way valve 5 is assembled on the inner wall of the overflow tube 3. A first guide plate 6 and a second guide plate 7 are fixedly connected at the connection between the inner wall of the sampling tube 1 and the overflow tube 3.
[0022] like Figures 2-4 As shown, the collection assembly includes a collection pipe 4 assembled at one end of the overflow pipe 3, a sealing cap 10 assembled at one end of the collection pipe 4, baffles 8 fixedly connected to the side walls of the overflow pipe 3 and the collection pipe 4 respectively, and a rubber sleeve 9 connected between the two baffles 8.
[0023] The collecting pipe 4 and the overflow pipe 3 are connected by a rubber sleeve 9. The elasticity of the rubber sleeve 9 makes it easy to tighten the collecting pipe 4 and the overflow pipe 3, so as to facilitate disassembly and docking. In addition, the inner surface of the rubber sleeve 9 fits tightly with the collecting pipe 4 and the overflow pipe 3, providing good sealing performance.
[0024] like Figure 2 As shown, the first guide plate 6 and the second guide plate 7 are arranged in parallel with downward inclination. The second guide plate 7 is located above the first guide plate 6, and the end of the second guide plate 7 near the overflow pipe 3 is arc-shaped.
[0025] Specifically, the width of the second guide plate 7 is half the inner diameter of the sampling tube 1. When excess solution comes into contact with the second guide plate 7 after passing through the first guide plate 6, the pressure generated by the rebound of the solution after being squeezed by the cap 2 will move it upward. After the solution leaves the second guide plate 7, it falls onto the first guide plate 6 and the overflow pipe 3 by its own gravity, and enters the collection pipe 4 along the inclined surface of the overflow pipe 3, effectively avoiding contamination caused by the solution entering the cap 2.
[0026] like Figure 2 As shown, a shielding layer 11 is fixedly connected to the inner wall of the sampling tube 1 near the top. The shielding layer 11 can be made of materials such as high-density microporous membrane, composite material, and polytetrafluoroethylene film, which can reduce solution penetration while ensuring airflow and provide secondary protection against solution overflow.
[0027] A scale 12 is provided on the side wall of the sampling tube 1. By setting the scale 12, it is easy to observe the amount of solution inside the sampling tube 1 in real time, and to intuitively control the amount of solution added at one time, so as to provide a more accurate ratio for experiments and tests.
[0028] like Figure 1 and Figure 2 As shown, the overflow pipe 3 and the collection pipe 4 are arranged at a downward angle. This is to facilitate the diversion of excess solution into the overflow pipe 3 and the collection pipe 4, thus serving the functions of diversion and collection.
[0029] The working principle of this utility model is as follows: When using a dropper to select a solution, if the amount of solution in the sampling tube 1 is too large, the solution first contacts the first guide plate 6, and then contacts the second guide plate 7. When the solution reaches one end of the second guide plate 7, the downward arc surface of the second guide plate 7 guides the solution into the overflow tube 3. At the same time, the one-way valve 5 opens, and the excess solution flows into the collection tube 4. This can avoid the pollution problem caused by the solution directly entering the cap 2, and effectively improve its practicality.
[0030] The above description is merely a preferred embodiment of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model. Structures, devices, and operating methods not specifically described or explained in this utility model, unless otherwise specified or limited, shall be implemented using conventional methods in the art.
Claims
1. A dropper for use in a high-sensitivity endotoxin assay using Limulus recombinant factor C, characterized by: The utility model relates to a sampling tube and rubber cap (2) are included, rubber cap (2) is assembled in sampling tube (1) top, sampling tube (1) side wall fixed connection has overflow pipe (3), overflow pipe (3) other end is equipped with collection subassembly, overflow pipe (3) inner wall is equipped with check valve (5), sampling tube (1) inner wall and overflow pipe (3) junction place fixed connection has first deflector (6) and second deflector (7).
2. The dropper for high-sensitivity endotoxin detection of Limulus recombinant C factor according to claim 1, characterized in that: The collection subassembly includes a collection tube (4) assembled at one end of the overflow pipe (3), the collection tube (4) is equipped with a sealing cover (10) at one end, the overflow pipe (3) and the collection tube (4) are respectively fixedly connected with baffles (8) on the side walls, and the two baffles (8) are connected with a rubber sleeve (9).
3. The dropper for high sensitive endotoxin detection of Limulus recombinant C factor according to claim 1, characterized in that: The first deflector (6) and the second deflector (7) are downwardly inclined and parallel, the second deflector (7) is above the first deflector (6), and the second deflector (7) is arc-shaped near one end of the overflow pipe (3).
4. The dropper for high sensitive endotoxin detection of Limulus recombinant C factor according to claim 1, characterized in that: The sampling tube (1) is fixedly connected with a shielding layer (11) near the top end on the inner wall.
5. The dropper for high sensitive endotoxin detection of Limulus recombinant C factor according to claim 1, characterized in that: The sampling tube (1) is provided with a scale (12) on the side wall.
6. The dropper for high sensitive endotoxin detection of Limulus recombinant C factor according to claim 2, characterized in that: The overflow pipe (3) and the collection tube (4) are downwardly inclined.