Combined reagent kit for DNA purification experiment

Abstract

The utility model provides a combined reagent kit for DNA (deoxyribonucleic acid) purification experiment, which comprises a box body, a damping rotating shaft is arranged on the bottom wall of the box body, the bottom wall of the box body is rotatably connected with a classified storage disc through the damping rotating shaft, and a driving component for driving the classified storage disc to rotate is arranged at the bottom of the box body. A storage battery used for supplying power to the driving assembly is arranged in the box body. An upper cover is rotationally connected to an upper opening of the box body, a fan-shaped hole is formed in the top of the upper cover in a penetrating mode, a fan-shaped cover plate used for closing the fan-shaped hole is rotationally connected into the upper cover, and a rotating assembly used for fixing the opening and closing position of the upper cover is arranged on the side wall of the upper cover; according to the utility model, by arranging the classified storage trays, different types of reagents can be stored in a classified manner; by arranging the driving assembly, automatic rotation of the classified storage disc is achieved, and a user can easily select needed reagents; by arranging the rotating assembly, the upper cover can be opened and closed, and the reagent can be integrally replaced conveniently.

Description

Technical Field

[0001] This invention relates to the field of DNA purification, and more particularly to a combination reagent kit for DNA purification experiments. Background Technology

[0002] In biological DNA purification experiments, the centrifugal column method is commonly used. This method requires adding various reagents, such as equilibration buffer (BL), binding buffer (PB), wash buffer (PW), and elution buffer (EB), sequentially to the DNA purification tube, followed by multiple centrifugations to remove precipitates, ultimately yielding a highly pure DNA solution. DNA purification often requires numerous purification experiments for different samples, necessitating the addition of reagents in batches across multiple purification tubes to improve efficiency.

[0003] However, due to the large number of reagents required and the need to conduct multiple experiments, the reagents are often misplaced, and mixing multiple reagents together can easily lead to mistakes in picking or packing them. Therefore, there is an urgent need for a combination reagent kit for DNA purification experiments that allows for the classification and easy access of reagents. Utility Model Content

[0004] To address the problem of incorrect selection or packaging of multiple reagents when they are mixed together in existing technologies, this invention provides a combined reagent kit for DNA purification experiments.

[0005] This utility model provides a combined reagent kit for DNA purification experiments, which adopts the following technical solution:

[0006] A combination reagent kit for DNA purification experiments includes a box body with a damping pivot on the bottom wall. A sorting tray is rotatably connected to the bottom wall of the box body via the damping pivot. A drive assembly for rotating the sorting tray is located at the bottom of the box body. A battery for powering the drive assembly is located inside the box body. A top cover is rotatably connected to the upper opening of the box body. A fan-shaped hole is formed through the top of the top cover. A fan-shaped cover plate for closing the fan-shaped hole is rotatably connected inside the top cover. A rotating assembly for fixing the opening and closing position of the top cover is located on the side wall of the top cover.

[0007] Furthermore, the top of the classification storage tray is provided with four sector-shaped storage areas, each sector-shaped storage area has a central angle of 90 degrees, and each sector-shaped storage area has several storage holes on its top.

[0008] Furthermore, the drive assembly includes a servo motor, an intermittent turntable, a turntable, and a lever; the servo motor is fixedly installed at the bottom of the housing, the output end of the servo motor is fixedly connected to the turntable, a lever is provided at the top of the turntable near the edge, the intermittent turntable is fixedly connected to the outer wall of the damping shaft, and a lever groove is provided on the side wall of the intermittent turntable, the lever can cooperate with the lever groove to drive the damping shaft to rotate.

[0009] Furthermore, the rotating assembly includes a sliding column, a spring, and a bolt cap; the outer side wall of the upper cover is fixedly connected to the sliding column, the outer side wall of the sliding column is slidably connected to the bolt cap, one end of the spring is fixedly connected to the outer side wall of the upper cover, the other end of the spring is fixedly connected to the bolt cap, and a sliding groove is provided through the side wall of the box body, and the bolt cap can slide along the sliding groove.

[0010] Furthermore, the cap includes a first pin portion and a second pin portion, which are integrally formed. The diameter of the second pin portion is larger than the diameter of the first pin portion. The width of the groove is larger than the diameter of the first pin portion and smaller than the diameter of the second pin portion. Pin holes are respectively provided at both ends of the groove, and the pin holes can cooperate with the second pin portion to fix the position of the top cover.

[0011] Furthermore, a stop plate is integrally formed at one end of the second pin portion located inside the box body, and the diameter of the stop plate is larger than the diameter of the pin hole.

[0012] In summary, the beneficial effects of this utility model are as follows:

[0013] This invention features a categorized storage tray, allowing different types of reagents to be stored separately, preventing errors in reagent selection or placement. A drive component enables automatic rotation of the storage tray, allowing users to easily select the required reagents without manual searching, thus improving the convenience of experimental operations. The rotating mechanism allows the top cover to be both open and closed, enabling complete reagent replacement after the experiment is finished. Attached Figure Description

[0014] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0015] Figure 2 This is a cross-sectional structural diagram of the present invention;

[0016] Figure 3 This is a top view cross-sectional structural diagram of the top cover of this utility model;

[0017] Figure 4 This is a top view of the classification and storage tray structure of this utility model;

[0018] Figure 5 This is a top view cross-sectional structural diagram of the drive component of this utility model;

[0019] Figure 6 This is a front view cross-sectional structural diagram of the rotating component of this utility model;

[0020] Figure 7 This utility model Figure 1 Enlarged schematic diagram of structure A in the middle;

[0021] Figure 8 This is a schematic diagram of the cap structure of this utility model.

[0022] As shown in the figure: 1-box body, 2-damping shaft, 3-classification storage tray, 31-fan-shaped storage area, 32-storage hole, 4-battery, 5-top cover, 51-fan-shaped hole, 52-fan-shaped cover plate, 61-servo motor, 62-intermittent turntable, 63-turntable, 64-lever, 65-lever groove, 71-sliding column, 72-spring, 73-bolt cap, 731-first pin part, 732-second pin part, 733-stop plate, 74-sliding groove, 75-pin hole. Detailed Implementation

[0023] The following is in conjunction with the appendix Figure 1 - Appendix Figure 8 The present invention will be further described in detail below:

[0024] This utility model discloses a combination reagent kit for DNA purification experiments, such as... Figure 1 , Figure 2 , Figure 3 As shown, this utility model discloses a combination reagent kit for DNA purification experiments, comprising a box body 1, a damping rotating shaft 2 on the bottom wall of the box body 1, a sorting tray 3 rotatably connected to the bottom wall of the box body 1 via the damping rotating shaft 2, a driving component for driving the sorting tray 3 to rotate at the bottom of the box body 1, and a battery 4 for powering the driving component inside the box body 1; a top cover 5 rotatably connected to the upper opening of the box body 1, a fan-shaped hole 51 penetrating through the top of the top cover 5, a fan-shaped cover plate 52 rotatably connected inside the top cover 5 for closing the fan-shaped hole 51, and a rotating component for fixing the opening and closing position of the top cover 5 on the side wall; in this embodiment, the positions of the fan-shaped hole 51 and the fan-shaped cover plate 52 are as follows. Figure 3As shown, a sector-shaped chamber is formed on the inner wall of the sector-shaped hole 51. A sector-shaped cover plate 52 for closing the sector-shaped hole 51 is rotatably connected inside the sector-shaped chamber. The area of ​​the sector-shaped hole 51 is slightly larger than the area of ​​the sector-shaped storage area 31, which facilitates reagent retrieval and maintains the airtightness of the box 1 when not in use. By setting up a driving component and a classification storage tray 3, the automatic rotation of the classification storage tray 3 is achieved, allowing users to easily select the required reagents without manual searching, improving the convenience of experimental operations. The rotating component allows the top cover 5 to be in both open and closed states. After the experiment is completed, the top cover 5 can be opened to replace all reagents. The classification storage tray 3 allows different types of reagents to be stored separately, preventing the misuse or incorrect loading / unloading of reagents.

[0025] like Figure 4 As shown, the top of the classification storage tray 3 is provided with four sector-shaped storage areas 31, each with a central angle of 90 degrees. Each sector-shaped storage area 31 has several storage holes 32 at its top. In this embodiment, there are four sector-shaped storage areas 31, corresponding to the equilibration buffer BL, binding buffer PB, washing buffer PW, and elution buffer EB used in the purification process. The number of sector-shaped storage areas 31 can also be set differently according to the needs of different purification methods. In this purification method, four types of reagents are required. The sector-shaped storage areas 31 correspond to the sector-shaped holes 51, through which reagents on the surface of the sector-shaped storage area 31 can be retrieved. By setting four sector-shaped storage areas 31, each with storage holes 32, different types of reagents can be stored in an orderly manner, avoiding confusion and mispacking. Preferably, the type of reagent in each sector-shaped storage area 31 can be determined by setting different colors for different sector-shaped storage areas 31, or by affixing corresponding type labels to the sector-shaped storage areas 31.

[0026] like Figure 2 , Figure 5 As shown, the drive assembly includes a servo motor 61, an intermittent turntable 62, a turntable 63, and a lever 64. The servo motor 61 is fixedly mounted on the bottom of the housing 1. The output end of the servo motor 61 is fixedly connected to the turntable 63. A lever 64 is located near the edge of the top of the turntable 63. The intermittent turntable 62 is fixedly connected to the outer wall of the damping shaft 2. A lever groove 65 is provided on the side wall of the intermittent turntable 62, and the lever 64 can cooperate with the lever groove 65 to drive the damping shaft 2 to rotate. In this embodiment, the specific shape of the intermittent turntable 62 and the position of the lever 64 are as follows: Figure 5As shown. Battery 4 powers servo motor 61. The number of lever slots 65 on intermittent turntable 62 is the same as the number of sector storage areas 31. Every time turntable 63 rotates, lever 64 enters lever slot 65 once, thereby driving intermittent turntable 62 to rotate at a certain angle, so that sector storage area 31 in sector hole 51 is switched to the next sector storage area 31.

[0027] like Figure 6 , Figure 7 As shown, the rotating assembly includes a sliding column 71, a spring 72, and a cap 73; the sliding column 71 is fixedly connected to the outer wall of the upper cover 5, the cap 73 is slidably connected to the outer wall of the sliding column 71, one end of the spring 72 is fixedly connected to the outer wall of the upper cover 5, and the other end of the spring 72 is fixedly connected to the cap 73; a sliding groove 74 is provided through the side wall of the box body 1, and the cap 73 can slide along the sliding groove 74; in this embodiment, the positions of the sliding column 71 and the spring 72 are as follows. Figure 6 As shown, the shape of the groove 74 is as follows Figure 7 As shown. Spring 72 is sleeved on the outside of slide post 71. One end of spring 72 is connected to the end of cap 73 located inside the housing 1, and the other end of spring 72 is connected to the outer wall of the upper cover 5. By providing a sliding groove 74, slide post 71 and cap 73 can cooperate with sliding groove 74, thereby allowing the upper cover 5 to be in a vertically open state and a horizontally closed state.

[0028] like Figure 8 As shown, the cap 73 includes a first pin portion 731 and a second pin portion 732, which are integrally formed. The diameter of the second pin portion 732 is larger than the diameter of the first pin portion 731. The width of the sliding groove 74 is larger than the diameter of the first pin portion 731 and smaller than the diameter of the second pin portion 732. Pin holes 75 are respectively provided at both ends of the sliding groove 74, which can cooperate with the second pin portion 732 to fix the position of the top cover 5. A stop piece 733 is integrally formed at one end of the second pin portion 732 located inside the box body 1. The diameter of the stop piece 733 is larger than the diameter of the pin hole 75. In this embodiment, the specific shape of the cap 73 is as follows: Figure 8 As shown, both the first pin portion 731 and the second pin portion 732 are cylindrical, with a frustum-shaped transition section between them. The diameter of the pin hole 75 is slightly larger than the diameter of the second pin portion 732, the diameter of the second pin portion 732 is larger than the width of the slide groove 74, and the width of the slide groove 74 is larger than the diameter of the first pin portion 731. Therefore, the second pin portion 732 cannot slide within the slide groove 74, but when the second pin portion 732 enters the pin hole 75, it is fixed in the pin hole 75, thus fixing the upper cover 5 in a fixed position. The positioning of the two pin holes 75 allows the upper cover 5 to be fixed vertically and horizontally.

[0029] The implementation principle of this utility model embodiment is as follows:

[0030] First, rotate the sector-shaped cover plate 52 to open the sector-shaped hole 51, and take out the reagent on the surface of the sector-shaped storage area 31 through the sector-shaped hole 51;

[0031] Next, the servo motor 61 is started, and the turntable 63 starts to rotate. Every time the turntable 63 rotates once, the lever 64 will enter the lever slot 65 once, which will drive the intermittent turntable 62 to rotate, so that the sector storage area 31 in the sector hole 51 is switched to the next sector storage area 31 and the reagent on the surface of the sector storage area 31 is taken through the sector hole 51.

[0032] Next, repeat the above steps to use up all the reagents until the experiment is over;

[0033] Finally, when all reagents need to be destroyed after the experiment, press the cap 73 to allow the first pin 731 to enter the pin hole 75, open the top cover 5, and when the first pin 731 moves along the slide groove 74 to the pin hole 75 at the other end, the spring 72 will push the second pin 732 into the pin hole 75 to fix the top cover 5. Then, remove the reagents from all the fan-shaped storage areas 31 on the surface of the classification storage tray 3 for destruction and replacement.

[0034] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. The various components mentioned in this utility model are common technologies in the existing field. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A combination reagent kit for DNA purification experiments, comprising a housing (1), characterized in that: The bottom wall of the box (1) is provided with a damping shaft (2), and the bottom wall of the box (1) is rotatably connected to a sorting storage tray (3) through the damping shaft (2). The bottom of the box (1) is provided with a drive component for driving the sorting storage tray (3) to rotate. The inside of the box (1) is provided with a battery (4) for powering the drive component. The upper opening of the box (1) is rotatably connected with a top cover (5). The top of the top cover (5) is provided with a fan-shaped hole (51). The inside of the top cover (5) is rotatably connected with a fan-shaped cover plate (52) for closing the fan-shaped hole (51). The side wall of the top cover (5) is provided with a rotating component for fixing the opening and closing position of the top cover (5).

2. The combined reagent kit for DNA purification experiments according to claim 1, characterized in that: The top of the classification storage tray (3) is provided with four fan-shaped storage areas (31), each fan-shaped storage area (31) has a central angle of 90 degrees, and each fan-shaped storage area (31) has several storage holes (32) on its top.

3. The combined reagent kit for DNA purification experiments according to claim 1, characterized in that: The drive assembly includes a servo motor (61), an intermittent turntable (62), a turntable (63), and a lever (64). The servo motor (61) is fixedly installed at the bottom of the housing (1). The output end of the servo motor (61) is fixedly connected to the turntable (63). A lever (64) is provided at the top of the turntable (63) near the edge. The intermittent turntable (62) is fixedly connected to the outer wall of the damping shaft (2). A lever groove (65) is provided on the side wall of the intermittent turntable (62). The lever (64) can cooperate with the lever groove (65) to drive the damping shaft (2) to rotate.

4. The combined reagent kit for DNA purification experiments according to claim 1, characterized in that: The rotating assembly includes a sliding column (71), a spring (72), and a cap (73); the outer side wall of the upper cover (5) is fixedly connected to the sliding column (71), the outer side wall of the sliding column (71) is slidably connected to the cap (73), one end of the spring (72) is fixedly connected to the outer side wall of the upper cover (5), the other end of the spring (72) is fixedly connected to the cap (73), and a sliding groove (74) is provided through the side wall of the box body (1), and the cap (73) can slide along the sliding groove (74).

5. A combination reagent kit for DNA purification experiments according to claim 4, characterized in that: The cap (73) includes a first pin portion (731) and a second pin portion (732), which are integrally formed. The diameter of the second pin portion (732) is larger than that of the first pin portion (731). The width of the groove (74) is larger than that of the first pin portion (731) and smaller than that of the second pin portion (732). Pin holes (75) are respectively provided at both ends of the groove (74). The pin holes (75) can cooperate with the second pin portion (732) to fix the position of the top cover (5).

6. A combination reagent kit for DNA purification experiments according to claim 5, characterized in that: The second pin portion (732) has a stop piece (733) integrally formed at one end inside the box body (1), and the diameter of the stop piece (733) is larger than the diameter of the pin hole (75).

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