Demolding clamp for resin embedded block

Abstract

The utility model discloses a demoulding clamp for a resin embedding block. The demoulding clamp comprises a lining, a pressing sleeve and a sealing cover, the upper part of the bushing is of a cylindrical structure with an external thread, the lower part of the bushing is of a cone frustum structure with a gradually reduced diameter, and a bracket for fixing the centrifugal tube is arranged in an inner cavity of the cylindrical structure and the cone frustum structure; a plurality of counterbores are uniformly formed in the conical surface of the cone frustum structure, and bolts facing an inner cavity of the cone frustum structure are arranged in the counterbores in a penetrating manner; an inner cavity of the pressing sleeve is matched with the outer wall face of the lining in shape. The sealing cover is in threaded fit with the cylindrical structure on the upper portion of the lining, and an inner hole for a self-tapping screw (7) to penetrate through is formed in the center of the sealing cover.

Description

Technical Field

[0001] This utility model relates to the field of experimental instruments, and in particular to a demolding fixture for resin-embedded blocks. Background Technology

[0002] In the field of life sciences, observing the internal structure of organisms using electron microscopy usually requires embedding biological tissues in resin to obtain ultrathin sections.

[0003] For example, Chinese patent document CN112393964A discloses a method for preparing biological single-cell transmission electron microscopy samples. The method involves placing biological single-cell culture medium and sterile lead-containing solution together in a culture medium, sealing and shaking them, and then culturing them to obtain a sample solution. The cultured sample solution is then subjected to fixation, rinsing, dehydration, embedding, and sectioning to obtain a TEM sample.

[0004] Chinese patent document CN114062092A discloses a plastic embedding method that simultaneously maintains multicolor fluorescence signals and ultrastructure, comprising: pre-treating fluorescently labeled biological tissue samples; immersing the pre-treated biological samples in a gradient of ethanol and embedding agent; and polymerizing the gradient-treated samples at -20℃ to 4℃ using an embedding agent containing a low-temperature polymerization accelerator to obtain embedded samples. By adding a low-temperature polymerization accelerator, the purpose of achieving low-temperature and stable polymerization of biological tissue samples is achieved.

[0005] In the routine preparation of biological samples, centrifuge tubes made of polypropylene (PP) are commonly used as embedding molds. The liquid resin is cured in a constant temperature incubator at 60-80℃ for 12-24 hours, completing the embedding of biological tissues. Because the cured resin embedding block is tightly bonded to the centrifuge tube, the conventional method involves immersing it in liquid nitrogen, using thermal expansion and contraction to demold it, and then cutting open the centrifuge tube to remove the sample. However, this method is time-consuming, costly, and the use of liquid nitrogen poses certain safety risks. Utility Model Content

[0006] This invention provides a demolding fixture for resin-embedded blocks, which is simple and convenient to operate and can achieve safe and efficient demolding operations.

[0007] The technical solution of this utility model is as follows:

[0008] A demolding fixture for resin-embedded blocks includes a bushing, a pressure sleeve, and a cap.

[0009] The upper part of the bushing is a cylindrical structure with external threads, and the lower part is a truncated cone structure with a gradually decreasing diameter. The inner cavities of the cylindrical structure and the truncated cone structure are provided with supports for fixing centrifuge tubes. Several countersunk holes are evenly provided on the conical surface of the truncated cone structure, and bolts facing the inner cavity of the truncated cone structure are inserted into the countersunk holes.

[0010] The inner cavity of the pressing sleeve is matched with the outer wall surface of the bushing; the cover is screwed with the cylindrical structure of the upper part of the bushing, and the center of the cover is provided with an inner hole for the self-tapping screw to pass through.

[0011] The utility model discloses a device for biological sample embedding and demolding, which is suitable for rapid demolding and sampling after biological sample embedding.

[0012] Preferably, the pressing sleeve, bolt, bushing, self-tapping screw rod and cover are made of stainless steel.

[0013] Preferably, a spring is arranged between the bolt and the outer taper surface of the conical structure.

[0014] Preferably, three countersunk holes are uniformly arranged on the taper surface of the conical structure.

[0015] The length of the bolt can be selected according to the volume of the centrifugal tube, and preferably, the length of the bolt is 1 / 4 to 1 / 3 of the outer diameter of the cylindrical structure of the bushing.

[0016] Preferably, the material can be selected from 65Mn (spring steel), and the length of the spring is 1 / 2 to 4 / 5 of the length of the bolt.

[0017] Preferably, the inner wall of the pressing sleeve is provided with an inner thread at the upper part, and a tapered wall at the lower part, which is used to push against the bolt.

[0018] Preferably, the inner hole diameter of the cover is greater than the inner diameter of the resin embedding block by 0.5 mm, which is to ensure that the resin embedding block can be smoothly taken out through the inner hole of the cover and will not be stuck due to small hole.

[0019] Compared with the prior art, the utility model has the following beneficial effects:

[0020] The utility model discloses a bolt extruding centrifugal tube, utilizing elastic plastic deformation, making resin embedding block and centrifugal tube produce local gap and demolding, then using self-tapping screw rod to attack resin embedding block, finally pulling out self-tapping screw rod and taking out resin embedding block, which saves the use of liquid nitrogen, saves cost, and is simple, convenient and safe in operation. BRIEF DESCRIPTION OF DRAWINGS

[0021] To more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Fig. 1 This is a cross-sectional view of a demolding fixture for resin-embedded blocks according to the present invention.

[0023] Fig. 2 This is a schematic diagram of the bushing structure in this utility model.

[0024] Fig. 3 This is a schematic diagram of the structure of the pressure sleeve in this utility model.

[0025] Fig. 4 This is a schematic diagram of the structure of the cap in this utility model.

[0026] In the diagram: 1-pressure sleeve, 11-internal thread, 12-inner conical wall, 2-bolt, 3-spring, 4-shield, 41-external thread, 42-countersunk hole, 5-centrifuge tube, 6-resin embedding block, 7-self-tapping screw, 8-cap, 81-inner hole, 82-internal thread. Detailed Implementation

[0027] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be noted that the embodiments described below are intended to facilitate the understanding of the present invention and do not limit it in any way.

[0028] like Figs. 1-4 As shown, a demolding fixture for resin embedded blocks includes a pressure sleeve 1, a bolt 2, a spring 3, a bushing 4, a centrifuge tube 5, a self-tapping screw 7, and a cap 8.

[0029] The pressure sleeve 1, bolt 2, bushing 4, self-tapping screw 7, and cover 8 are all made of stainless steel.

[0030] The upper part of the bushing 4 is a cylindrical structure with external threads 41, and the lower part is a truncated cone structure with a gradually decreasing diameter. The inner cavities of the cylindrical structure and the truncated cone structure are equipped with supports for fixing the centrifuge tube 5. Three countersunk holes 42 are evenly provided on the conical surface of the truncated cone structure. Bolts 2 are inserted into the countersunk holes 42 and face the inner cavity of the truncated cone structure. A spring 3 is assembled between the bolt 2 and the countersunk hole 42.

[0031] The inner cavity of the pressure sleeve 1 matches the outer wall shape of the bushing 4, and is connected to the bushing 4 by threads, with the inner conical wall pressing against the bolt.

[0032] The cover 8 is connected with the bushing 4 through the internal thread 82, and the center of the cover 8 is provided with an inner hole 81 for the self-tapping screw 7 to pass through, and the self-tapping screw 7 is coaxially matched with the cover 8.

[0033] The length of the bolt 2 can be selected according to the volume of the centrifugal tube 5, and in the embodiment, the length of the bolt 2 is 1 / 3 of the outer diameter of the cylindrical structure of the bushing 4.

[0034] The material of the spring 3 is selected to be 65Mn, and the length of the spring 3 is 1 / 2 of the length of the bolt 2.

[0035] The diameter of the inner hole 81 of the cover 8 should be greater than the inner diameter of the resin embedding block 6 by 0.5mm.

[0036] The working principle of the utility model is as follows:

[0037] The bolt 2 is used to extrude the centrifugal tube 5, the elastic-plastic deformation is utilized, the resin embedding block 6 and the centrifugal tube 5 generate a local gap to demould, the self-tapping screw 7 is used to attack into the resin embedding block 6, and finally the self-tapping screw 7 is pulled out to take out the resin embedding block 6.

[0038] Next, taking the 1.5ml centrifugal tube as an example, the operation process of the utility model is introduced.

[0039] 1. First, rotate the cover counterclockwise.

[0040] 2. Put the 1.5ml centrifugal tube containing the resin embedding block 6 into the center hole of the bushing 4, and the support of the bushing 4 supports the centrifugal tube.

[0041] 3. Rotate the cover 8 clockwise and press the 1.5ml centrifugal tube.

[0042] 4. Hold the bushing 4, rotate the pressure sleeve 1 clockwise, so that the bolt 2 gradually extrudes the 1.5ml centrifugal tube, and after extruding 2-3mm, the centrifugal tube 5 and the resin embedding block 6 are demoulded.

[0043] 5. Attack the self-tapping screw 7 into the resin embedding block 6, attack the length of 15-25mm, pull out the self-tapping screw 7 to take out the resin embedding block 6.

[0044] The above-described embodiments have described the technical solutions and beneficial effects of the utility model in detail, and it should be understood that the above-described only for the specific embodiments of the utility model, and is not used to limit the utility model, any modification, supplement and equivalent replacement made in the principle range of the utility model should be included in the protection range of the utility model.

Claims

1. A demolding jig for a resin-embedded block, characterized by, It comprises a bushing (4), a pressing sleeve (1) and a cover (8); The upper part of the bushing (4) is a cylindrical structure with external thread, and the lower part is a conical frustum structure with gradually decreasing diameter, and the inner cavity of the cylindrical structure and the conical frustum structure is provided with a support for fixing a centrifugal tube (5); the conical surface of the conical frustum structure is uniformly provided with a plurality of countersunk holes (42), and a bolt (2) is arranged in the countersunk hole (42) and faces the inner cavity of the conical frustum structure; The inner cavity of the pressing sleeve (1) is matched with the outer wall surface shape of the bushing (4); the cover (8) is threadedly matched with the cylindrical structure of the upper part of the bushing (4), and the center of the cover (8) is provided with an inner hole (81) for passing through a self-tapping screw (7).

2. The demolding jig for a resin-embedded block according to Claim 1, wherein The spring (3) is arranged between the bolt (2) and the outer conical surface of the conical frustum structure.

3. The demolding jig for a resin-embedded block according to Claim 1, wherein The conical surface of the conical frustum structure is uniformly provided with three countersunk holes (42).

4. The demolding jig for a resin-embedded block according to Claim 1, wherein The length of the bolt (2) is 1 / 4-1 / 3 of the outer diameter of the cylindrical structure of the bushing (4).

5. The demolding jig for a resin-embedded block according to Claim 1, wherein The length of the spring (3) is 1 / 2-4 / 5 of the length of the bolt (2).

6. The demolding jig for a resin-embedded block according to Claim 1, wherein The inner wall of the pressing sleeve (1) is provided with internal thread at the upper part, and the lower part is a conical wall.

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