AM (arbuscular mycorrhizal) fungus circulating culture device
By designing an AM fungal circulating culture device and adopting a staged contact culture method with compartments near and far from the root system, the problem of low culture efficiency of traditional AM fungal agents was solved, achieving high-efficiency production of AM fungal agents and improving the yield and quality of the agents.
Patent Information
- Application Number
- CN202520136283.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-21
- Publication Date
- 2026-01-13
- Estimated Expiration
- 2035-01-21
AI Technical Summary
Traditional AM fungal inoculants have low cultivation efficiency, which limits their widespread application in modern agriculture.
An AM fungal cyclic culture device is designed. By setting up near-root compartments and far-root compartments, and utilizing the sliding and extraction mechanism of hyphal septa, AM fungi and plant roots can be cultured in stages. A porous nylon membrane is used to ensure normal contact between hyphae and roots, and efficient recycling is achieved through dynamic adjustment of the hyphal septa.
It significantly shortened the cultivation cycle of AM fungi, improved the production efficiency of the inoculant, realized the efficient recycling of AM fungal inoculant, and improved the yield and quality of the inoculant.
Smart Images
Figure CN223793123U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of agricultural biotechnology, and in particular to an AM fungal circulating culture device. Background Technology
[0002] Arbuscular mycorrhizae (AM) fungi are a class of soil microorganisms widely found in nature. They can promote the absorption of water and mineral nutrients by plants, significantly improving crop yield and stress resistance. However, traditional methods for cultivating AM fungal inoculants suffer from problems such as long cycles and low efficiency, limiting their widespread application in modern agriculture. Therefore, developing a device capable of rapidly cultivating AM fungal inoculants is of particular importance. Utility Model Content
[0003] The purpose of this invention is to provide an AM fungal circulating culture device to solve the problem of low culture efficiency of AM fungal agents in the prior art.
[0004] To achieve this objective, the present invention adopts the following technical solution:
[0005] This utility model discloses an AM fungal circulating culture device, comprising: a root chamber bottom plate for cultivating plants, wherein fixed perforated plates are provided on two side walls of the root chamber bottom plate arranged opposite each other along a first direction; and a mycelium chamber disposed on at least one side of the root chamber bottom plate along the first direction, wherein the mycelium chamber includes two mycelium partitions for cultivating AM fungi, wherein a movable perforated plate is provided between the two mycelium partitions, wherein the mycelium partitions are slidable along the first direction and can be pulled out of the mycelium chamber along a second direction.
[0006] In some embodiments, the mycelial chambers are provided on both sides of the root chamber floor plate along the first direction.
[0007] In some embodiments, the AM fungal circulating culture device further includes a side wall plate disposed on the side of the mycelial chamber away from the bottom plate of the root chamber.
[0008] In some specific embodiments, the AM fungal circulating culture device further includes a rear wall plate and a front wall plate. The two ends of the rear wall plate along the first direction are respectively connected to the two side wall plates. The movable perforated plate, the fixed perforated plate and the root chamber bottom plate all abut against the rear wall plate. The two ends of the front wall plate along the first direction are respectively connected to the two fixed perforated plates.
[0009] In some embodiments, the mycelium chamber further includes a slide rail extending along the first direction, and the two mycelium partitions abut against the slide rail.
[0010] In some embodiments, the movable perforated plate is sandwiched between the two mycelial septa.
[0011] In some embodiments, the movable perforated plate is detachably mounted on one of the two mycelial septa.
[0012] In some embodiments, both the movable perforated plate and the fixed perforated plate are porous nylon membranes.
[0013] In some embodiments, the pore size of the porous nylon membrane is 35μm-45μm.
[0014] In some embodiments, both the movable perforated plate and the fixed perforated plate are provided with handles.
[0015] The beneficial effects of this invention are as follows: Since the mycelial chamber comprises two mycelial septa, the septa closer to the root chamber floor form a near-root chamber, and the septa further from the root chamber floor form a far-root chamber. During actual cultivation, AM fungi grow first in the near-root chamber. This near-root chamber, being close to the plant roots, provides a suitable microenvironment, allowing the AM fungi to quickly adapt and establish connections with the roots. Mycelial growth occurs first in the far-root chamber. Due to its relative distance from the roots, the environment here is relatively stable in the initial stage, which is conducive to the large-scale reproduction of mycelia. After a specified period of growth culture in the near-root chamber, the mycelial septa are pulled out along a second direction to remove the structure containing mature AM fungi (such as the inoculum). Then, the mycelial septum that originally constituted the distal root compartment is moved along the first direction to a position close to the bottom plate of the root chamber, transforming it into a proximal root compartment. Then, a new mycelial septum is inserted as a new distal root compartment, and mycelial culture is restarted. In this way, AM fungi can be cultured continuously and efficiently in a limited space. Through the above dynamic adjustment, the AM fungi circulating culture device disclosed in this utility model realizes the efficient recycling of AM fungi inoculants, significantly shortens the culture cycle, and improves the production efficiency of inoculants.
[0016] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description
[0017] Figure 1 This is a schematic diagram of the AM fungal circulating culture device according to an embodiment of the present invention;
[0018] Figure 2 This is a partial structural schematic diagram of the AM fungal circulating culture device according to an embodiment of the present invention.
[0019] Figure label:
[0020] 100. Root chamber bottom plate; 200. Mycelial partition; 300. Fixed hole plate; 400. Movable hole plate; 500. Slide rail; 610. Side wall plate; 620. Rear wall plate; 630. Front wall plate; 700. Handle; 810. Near-root compartment; 820. Far-root compartment. Detailed Implementation
[0021] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present invention and not intended to limit it. Furthermore, it should be noted that, for ease of description, the accompanying drawings show only the parts relevant to the present invention, not the entire structure.
[0022] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.
[0023] In the description of this embodiment, the terms "upper," "lower," "left," "right," "front," and "rear," etc., refer to the orientation or positional relationship shown in the accompanying drawings. They are used only for ease of description and simplification of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are only used for distinction in description and have no special meaning.
[0024] This utility model discloses an AM fungal circulating culture device, reference Figure 1 and Figure 2As shown, the AM fungal circulating culture device includes a root chamber bottom plate 100 and a mycelium chamber. The root chamber bottom plate 100 is used to cultivate plants. Fixed perforated plates 300 are provided on two side walls of the root chamber bottom plate 100 that are arranged opposite each other along a first direction. The mycelium chamber is located on at least one side of the root chamber bottom plate 100 along the first direction. The mycelium chamber includes two mycelium partitions 200. The mycelium partitions 200 are used to cultivate AM fungi. A movable perforated plate 400 is provided between the two mycelium partitions 200. The mycelium partitions 200 can slide along the first direction and can be pulled out of the mycelium chamber along a second direction. Understandably, since the mycelial chamber comprises two mycelial septa 200, the mycelial septa 200 closer to the root chamber floor 100 forms a near-root chamber 810, and the mycelial septa 200 farther from the root chamber floor 100 forms a far-root chamber 820. In actual cultivation, AM fungi grow first in the near-root chamber 810. The near-root chamber 810, being close to the plant roots, provides a suitable microenvironment, allowing the AM fungi to quickly adapt and establish contact with the roots. Mycelial growth occurs first in the far-root chamber 820. Due to its relative distance from the roots, the environment here is relatively stable in the initial stage, which is conducive to the large-scale reproduction of mycelia. After a specified period of growth culture in the near-root chamber 810, the mycelial septa 200 are pulled out along the second direction to remove the structure containing mature AM fungi (such as the inoculum). Then, the mycelial partition 200 that originally constituted the distal root compartment 820 is pushed to move along the first direction, so that it moves to a position close to the root chamber bottom plate 100, transforming it into a proximal root compartment 810. Then, a new mycelial partition 200 is inserted as a new distal root compartment 820, and mycelial culture is restarted. In this way, AM fungi can be cultured continuously and efficiently in a limited space. Through the above dynamic adjustment, the AM fungi circulating culture device disclosed in this utility model realizes the efficient recycling of AM fungi inoculants, significantly shortens the culture cycle, and improves the production efficiency of inoculants.
[0025] Furthermore, the added movable perforated plate 400 ensures that the culture material on the two mycelial partitions 200 maintains a fixed shape, preventing the culture material on the two mycelial partitions 200 from completely mixing together, which would hinder the subsequent movement of the mycelial partitions 200. The fixed perforated plate 300 restricts the growth of plant roots toward the mycelial partitions 200, thereby preventing damage to the plant roots and plant death during the removal of the mycelial partitions 200.
[0026] refer to Figure 1 As shown, mycelial chambers are provided on both sides of the root chamber bottom plate 100 along the first direction. It can be understood that during plant growth, the plant roots will spread to both sides, and the presence of mycelial chambers on both sides of the root chamber bottom plate 100 along the first direction is beneficial to increasing the yield of AM fungi.
[0027] refer to Figure 1As shown, the AM fungal circulating culture device also includes a side wall plate 610 located on the side of the mycelium chamber away from the root chamber bottom plate 100. It can be understood that the added side wall plate 610 helps to prevent the culture material from falling off the mycelium partition 200, thus avoiding waste of culture material, and also restricts the culture space of the mycelium, preventing the mycelium from growing into the external environment outside the AM fungal circulating culture device.
[0028] refer to Figure 1 As shown, the AM fungal circulating culture device also includes a rear wall plate 620 and a front wall plate 630. The rear wall plate 620 is connected to two side wall plates 610 at both ends along the first direction. The movable perforated plate 400, the fixed perforated plate 300, and the root chamber floor plate 100 all abut against the rear wall plate 620. The front wall plate 630 is connected to two fixed perforated plates 300 at both ends along the first direction. It is understood that the added rear wall plate 620 and front wall plate 630 can restrict the growth space of the plant roots, preventing them from growing into the external environment outside the AM fungal circulating culture device. The added rear wall plate 620 and front wall plate 630 can also improve the deformation resistance of the entire AM fungal circulating culture device.
[0029] refer to Figure 1 As shown, the mycelium chamber also includes a slide rail 500, which extends along a first direction, and two mycelium partitions 200 abut against the slide rail 500. It is understood that by setting the slide rail 500, researchers can easily slide the mycelium partitions 200 during the actual cultivation process, thus facilitating their operation.
[0030] Optionally, the movable well plate 400 is sandwiched between two hyphal septa 200. This eliminates the need for additional connectors to link the movable well plate 400, simplifying the structure of the AM fungal circulating culture device.
[0031] Optionally, the movable well plate 400 can be detachably mounted on one of the two mycelial partitions 200. Understandably, since the mycelial partition 200 will be provided with nutrients for cultivating AM fungi, the lower end of the movable well plate 400 can be directly fixed with the nutrients, making it very convenient to remove the movable well plate 400 when needed.
[0032] Optionally, both the movable orifice plate 400 and the fixed orifice plate 300 are made of porous nylon membrane. It is understood that using porous nylon membrane for the movable orifice plate 400 and the fixed orifice plate 300 can avoid adverse effects on plants and AM fungi. Of course, in other embodiments of this invention, the movable orifice plate 400 and the fixed orifice plate 300 can be made of other materials according to actual needs, and are not limited to the nylon membrane of this embodiment.
[0033] Optionally, the pore size of the porous nylon membrane is 35μm-45μm. Specifically, the pore sizes of the porous nylon membrane are 35μm, 36μm, 37μm, 38μm, 39μm, 40μm, 41μm, 42μm, 43μm, 44μm, and 45μm. Of course, the pore size of the porous nylon membrane can also be other values within the 35μm-45μm range, and is not limited to the above limitations. If the pore size of the porous nylon membrane is too large, during the cultivation process, plant roots can easily extend into the mycelial chamber, which can easily damage the plant roots when the mycelial septum is removed, thus increasing the risk of plant death. If the pore size of the porous nylon membrane is too small, it will affect the normal contact between the mycelium and the plant roots, which is not conducive to the cultivation of AM fungi. In this embodiment, the pore size of the porous nylon membrane is controlled within 35μm-45μm. This can prevent plant roots from extending into the mycelium chamber, thus avoiding damage to the plant roots when the mycelium partition 200 is pulled out, while also ensuring normal contact between the mycelium and the plant roots, which is beneficial for the cultivation of AM fungi.
[0034] Optional, see reference Figure 2 As shown, both the movable perforated plate 400 and the fixed perforated plate 300 are equipped with handles 700. This allows for easy assembly and disassembly of the movable perforated plate 400, and also facilitates the installation of the fixed perforated plate 300.
[0035] The advantages of the AM fungal circulating culture device disclosed in this utility model are as follows:
[0036] First, by setting up a near-root compartment 810 and a far-root compartment 820, the AM fungus and plant roots were allowed to be cultured in stages, which effectively improved the culture efficiency of the AM fungus inoculant.
[0037] Second: By adjusting the mycelial partition 200, AM fungi in the near-root compartment 810 can quickly come into contact with wheat roots, simplifying the operation process and improving the convenience of cultivation.
[0038] Third: During the cultivation process, the distal root compartment 820 can be moved towards the center to become the proximal root compartment 810, and the mycelium of the distal root compartment 820 can be re-cultured, realizing the cyclical progress of the cultivation process. This further improves the yield and quality of AM fungal inoculant and increases the cultivation efficiency of AM fungi. Compared with traditional methods, a large amount of AM fungi can be obtained in a shorter time.
[0039] The following describes an example of the actual operation of the AM fungal circulating culture device disclosed in this utility model.
[0040] Example 1:
[0041] An appropriate amount of AM fungi was inoculated into the near-root compartment 810, and culture conditions were controlled to allow it to grow for 5 days initially. Simultaneously, mycelia were cultured in the far-root compartment 820, allowing them to extend and grow inwards. After 5 days, the mycelial septum 200 near the root chamber floor 100 was removed to form a fungal inoculum, yielding 70 spores / g. The mycelial septum 200 away from the root chamber floor 100 was then pushed towards the central root chamber floor 100, creating a new near-root compartment 810. Mycelia were then re-inoculated at the original location of the far-root compartment 820, creating a new far-root compartment 820, and the above culture process was continued for 3 days, yielding 120 spores / g.
[0042] Example 2:
[0043] An appropriate amount of AM fungi was inoculated into the near-root compartment 810, and culture conditions were controlled to allow it to grow for 3 days initially. Simultaneously, mycelia were cultured in the far-root compartment 820, allowing them to extend and grow inwards. After 3 days, the AM fungi in the near-root compartment 810 were brought into contact with the plant roots to form an inoculum with 60 spores / g. The far-root compartment 820 was then moved into the central wheat growth compartment, creating a new near-root compartment 810. Mycelia were then re-inoculated at the original location of the far-root compartment 820 to create a new far-root compartment 820, and the above culture process was continued for 3 days, yielding an inoculum with 90 spores / g.
[0044] Example 3:
[0045] An appropriate amount of AM fungi was inoculated into the near-root compartment 810, and culture conditions were controlled to allow it to grow for 5 days initially. Simultaneously, mycelia were cultured in the far-root compartment 820, allowing them to extend and grow inwards. After 5 days, the AM fungi in the near-root compartment 810 were brought into contact with the plant roots to form an inoculum, yielding an inoculum spore count of 70 spores / g. The far-root compartment 820 was then moved into the central wheat growth compartment, creating a new near-root compartment 810. Mycelia were then re-inoculated at the original location of the far-root compartment 820, creating a new far-root compartment 820, and the above culture process was continued for 5 days, yielding an inoculum spore count of 120 spores / g.
[0046] Through the above examples, the AM fungal culture device of this invention can achieve efficient cultivation of AM fungal agents, providing high-quality agents for wheat growth, and has broad application prospects.
[0047] In the description of this specification, references to terms such as "some embodiments," "other embodiments," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.
[0048] Obviously, the above embodiments of this utility model are merely examples for clearly illustrating the present utility model, and are not intended to limit the implementation of the present utility model. Those skilled in the art can make various obvious changes, readjustments, and substitutions without departing from the protection scope of this utility model. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of the claims of this utility model.
Claims
1. An apparatus for cyclic cultivation of AM fungi, characterized by, include: A root chamber bottom plate (100) is used for cultivating plants. The root chamber bottom plate (100) has two side walls that are arranged opposite each other along a first direction and are provided with fixing holes (300). The mycelium chamber is located on at least one side of the root chamber bottom plate (100) along the first direction. The mycelium chamber includes two mycelium partitions (200) for culturing AM fungi. A movable perforated plate (400) is provided between the two mycelium partitions (200). The mycelium partitions (200) are slidable along the first direction and can be pulled out along the second direction.
2. The AM fungal circulatory culture device according to claim 1, wherein, The mycelial chambers are provided on both sides of the root chamber bottom plate (100) along the first direction.
3. The AM fungal circulatory culture device according to claim 1, wherein The AM fungal circulating culture device also includes a side wall plate (610) disposed on the side of the mycelium chamber away from the root chamber bottom plate (100).
4. The AM fungal circulatory culture device according to claim 3, wherein The AM fungal circulating culture device further includes a rear wall plate (620) and a front wall plate (630). The rear wall plate (620) is connected to the two side wall plates (610) at both ends along the first direction. The movable perforated plate (400), the fixed perforated plate (300) and the root chamber bottom plate (100) all abut against the rear wall plate (620). The front wall plate (630) is connected to the two fixed perforated plates (300) at both ends along the first direction.
5. The AM fungal circulatory culture device according to any one of claims 1-4, wherein, The mycelium chamber also includes a slide rail (500) extending along the first direction, and the two mycelium partitions (200) abut against the slide rail (500).
6. The AM fungal circulatory culture device according to any one of claims 1-4, wherein, The movable perforated plate (400) is sandwiched between the two mycelial septa (200).
7. The AM fungal circulatory culture device according to any one of claims 1-4, wherein, The movable perforated plate (400) is detachably mounted on one of the two mycelial septa (200).
8. The AM fungal circulatory culture device according to any one of claims 1-4, wherein, Both the movable perforated plate (400) and the fixed perforated plate (300) are porous nylon membranes.
9. The AM fungal circulatory culture device according to claim 8, wherein, The porous nylon membrane has a pore size of 35μm-45μm.
10. The AM fungal circulatory culture device according to any one of claims 1-4, wherein, Both the movable perforated plate (400) and the fixed perforated plate (300) are provided with handles (700).