SRB culture medium with hydrogen sulfide gas chromogenic indication

Abstract

The utility model provides an SRB culture medium with hydrogen sulfide gas chromogenic indication, which relates to the technical field of microbiological detection and comprises a culture medium bottle. A rapid detection assembly; the rapid detection assembly comprises a bottom retention ring fixedly connected to the inner bottom end of the culture medium bottle, the top end of the bottom retention ring is fixedly connected with a microporous film, and chromogenic gas is arranged between the bottom retention ring and the microporous film and is hydrogen sulfide gas; according to the design, an experimenter can directly observe the generation of hydrogen sulfide in the SRB metabolism process through a chromogenic reaction, and does not need to wait for a long-time reaction of a traditional detection method, so that the experimental period is greatly shortened; and the reaction between the hydrogen sulfide gas and the chromogenic gas is quicker and more sensitive due to the large contact area between the microporous film and the chromogenic gas, so that the sensitivity of the chromogenic reaction is remarkably improved, and the metabolic condition of SRB is more accurate.

Description

Technical Field

[0001] This invention relates to the field of microbial detection technology, and in particular to an SRB culture medium with hydrogen sulfide gas colorimetric indicator. Background Technology

[0002] Sulfate-reducing bacteria (SRB) are a class of microorganisms that can reduce sulfate to hydrogen sulfide in anaerobic environments. They are widely found in natural environments, such as water bodies, soil, and industrial wastewater. SRB play a crucial role in ecosystems, not only in biogeochemical cycles but also in water treatment, oil extraction, and other fields.

[0003] Currently, the cultivation of SRB generally relies on specialized culture media and corresponding culture conditions. Common SRB culture media typically contain basic components such as nutrients, carbon sources, and sulfates, and must be cultured under anaerobic conditions.

[0004] However, while existing SRB culture media can provide the necessary nutrients for monitoring SRB activity and the culture process in practical applications, traditional detection methods such as chemical reagent kits and gas chromatography are complex to operate and require long reaction times. They cannot monitor the generation of hydrogen sulfide in real time during the culture process, which in turn requires a long reaction time for manual sampling and analysis, thus easily introducing human error and affecting the accuracy of the culture results.

[0005] Therefore, this invention provides an SRB culture medium with a hydrogen sulfide gas colorimetric indicator. Utility Model Content

[0006] The purpose of this invention is to overcome the shortcomings of the existing technology and provide an SRB culture medium with hydrogen sulfide gas colorimetric indicator.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: an SRB culture medium with hydrogen sulfide gas colorimetric indicator, characterized in that it comprises;

[0008] Culture medium bottles;

[0009] A rapid detection component; the rapid detection component includes a bottom placement ring fixedly connected to the bottom of the inside of a culture medium bottle, a microporous membrane fixedly connected to the top of the bottom placement ring, and a color-developing gas, namely hydrogen sulfide gas, disposed between the bottom placement ring and the microporous membrane.

[0010] The technical effect of adopting the above technical solution is that, since the contact area between the color-developing gas and the microporous membrane is greater than 50% of the surface area of ​​the microporous membrane, when SRB metabolizes and produces hydrogen sulfide gas, the hydrogen sulfide can quickly pass through the microporous membrane and react with the color-developing gas, triggering a color change. This rapid color reaction allows the experimenter to observe the generation of hydrogen sulfide in real time without having to wait for a long reaction time.

[0011] Preferably, the top of the culture medium bottle has a threaded groove, and a sealing cap is threadedly connected to the top of the culture medium bottle through the threaded groove.

[0012] The technical effect of adopting the above technical solution is to ensure that the culture medium bottle is in a sealed state.

[0013] Preferably, an observation plate is fixedly connected to the outside of the culture medium bottle.

[0014] The technical effect of adopting the above technical solution is that the design of the observation outer plate allows the experimenters to observe continuously.

[0015] Preferably, the microporous membrane is made of polymethyl methacrylate.

[0016] The technical effect of adopting the above technical solution is that the microporous membrane made of polymethyl methacrylate has good biocompatibility, ensuring that hydrogen sulfide gas can pass through the membrane smoothly and react with the colorimetric gas.

[0017] Preferably, the contact area between the color-developing gas and the microporous membrane is greater than 50% of the surface area of ​​the microporous membrane.

[0018] The technical effect of adopting the above technical solution is that it can ensure that more colorimetric gas molecules are adsorbed and reacted, thereby improving the detection sensitivity.

[0019] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0020] This invention utilizes a combination of a microporous membrane and a color-developing gas. Hydrogen sulfide gas produced inside the culture medium bottle during SRB metabolism reacts rapidly with the color-developing gas, triggering a color change. The porous structure of the microporous membrane provides a large contact area for rapid hydrogen sulfide gas penetration, enhancing the reaction rate between the gas and the color-developing gas, thereby improving the sensitivity and speed of the colorimetric reaction. This design allows researchers to directly observe the generation of hydrogen sulfide during SRB metabolism through the colorimetric reaction, eliminating the need to wait for the lengthy reaction time of traditional detection methods. This significantly shortens the experimental cycle. Furthermore, the large contact area between the microporous membrane and the color-developing gas makes the reaction between hydrogen sulfide gas and the color-developing gas more rapid and sensitive, significantly improving the sensitivity of the colorimetric reaction and making the SRB metabolism more accurate. Attached Figure Description

[0021] Figure 1 A three-dimensional view of an SRB culture medium with hydrogen sulfide gas colorimetric indicator provided by this utility model;

[0022] Figure 2 A cross-sectional schematic diagram of the structure of an SRB culture medium with hydrogen sulfide gas colorimetric indicator provided by this utility model;

[0023] Figure 3 A schematic diagram of the structure of a rapid detection component for SRB culture medium with hydrogen sulfide gas colorimetric indicator provided by this utility model;

[0024] Figure 4 A schematic diagram of the colorimetric gas structure of an SRB culture medium with hydrogen sulfide gas colorimetric indicator provided by this utility model.

[0025] Legend:

[0026] 1. Culture medium bottle; 2. Sealing cap; 3. Observation plate;

[0027] 4. Rapid detection component; 41. Bottom placement ring; 42. Microporous membrane; 43. Colorimetric gas. Detailed Implementation

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0029] like Figure 1 - Figure 3 As shown, this embodiment provides a technical solution: an SRB culture medium with hydrogen sulfide gas colorimetric indicator, comprising;

[0030] Culture medium bottle 1, with a threaded groove at the top of the outer side of culture medium bottle 1, and a sealing cap 2 is threadedly connected to the top of culture medium bottle 1 through the threaded groove, and an observation outer plate 3 is fixedly connected to the outer side of culture medium bottle 1.

[0031] Culture medium bottle 1 serves as the container for the entire culture system, holding the culture medium, SRB cells, and chromogenic module, providing space for SRB growth and metabolism, avoiding interference from external oxygen, and ensuring normal SRB growth. The threaded groove is located at the top outer side of culture medium bottle 1 and works in conjunction with the sealing cap 2 to seal culture medium bottle 1. The sealing cap 2, connected by threads, can tightly seal culture medium bottle 1 to prevent gas leakage, while also facilitating disassembly and replacement, and simplifying experimental operations. The observation outer plate 3 is fixedly connected to the outside of culture medium bottle 1 and is used to observe color changes inside the culture medium. The observation outer plate 3 provides an intuitive observation window, allowing experimenters to easily monitor the color changes of the chromogenic module, thereby quickly determining the metabolic status of SRB.

[0032] like Figure 1 - Figure 4 As shown, the rapid detection component 4 includes a bottom placement ring 41 fixedly connected to the bottom of the culture medium bottle 1. A microporous membrane 42 is fixedly connected to the top of the bottom placement ring 41. The microporous membrane 42 is made of polymethyl methacrylate. A colorimetric gas 43, which is hydrogen sulfide gas, is disposed between the bottom placement ring 41 and the microporous membrane 42. The contact area between the colorimetric gas 43 and the microporous membrane 42 is greater than 50% of the surface area of ​​the microporous membrane 42.

[0033] The bottom placement ring 41 is fixedly connected to the bottom of the culture medium bottle 1 to support and fix the microporous membrane 42 and the colorimetric gas 43, ensuring the stability of the position of the microporous membrane 42 and the colorimetric gas 43. This prevents the colorimetric module from shifting or being damaged due to vibration or movement during the culture process, thereby ensuring the accuracy and reliability of the detection results. The microporous membrane 42 is located at the top of the bottom placement ring 41 and is in contact with the colorimetric gas 43. When SRB metabolism produces hydrogen sulfide gas, the hydrogen sulfide will permeate through the microporous membrane 42 and react with the colorimetric gas 43, triggering a color change. The porous structure of the microporous membrane 42 increases the contact area with the hydrogen sulfide gas, making it easier for hydrogen sulfide to penetrate into the colorimetric gas 43, thus accelerating the color change. The colorimetric reaction proceeds effectively due to the large contact area, even low concentrations of hydrogen sulfide gas can be detected, improving detection sensitivity. Polymethyl methacrylate (PMMA) material possesses excellent transparency and chemical stability, allowing for clear observation of color changes. Furthermore, it is not easily interfered with by other chemicals in the culture medium. The colorimetric gas 43 is hydrogen sulfide gas, and its contact area with the microporous membrane 42 is greater than 50% of the surface area of ​​the microporous membrane 42. When SRB metabolism produces hydrogen sulfide gas, the colorimetric gas 43 reacts with it, producing a color change. The large contact area allows the colorimetric gas 43 to react rapidly with hydrogen sulfide, achieving rapid color development and thus quickly indicating the metabolic status of SRB.

[0034] like Figure 1 - Figure 4 As shown:

[0035] In use: First, the culture medium bottle 1 serves as the container for the entire culture system, containing the culture medium, SRB cells, and colorimetric module. It ensures a healthy SRB growth environment free from oxygen interference. Through the combination of the threaded groove and the sealing cap 2, the culture medium bottle 1 achieves a sealed function, preventing leakage of external gases and facilitating operation and replacement by the experimenter. Next, the observation plate 3 on the outside of the culture medium bottle 1 provides an observation window, allowing the experimenter to visually monitor color changes within the culture medium and keep abreast of the colorimetric reactions during SRB metabolism, thus enabling rapid assessment of SRB metabolism. Then, the rapid detection component 4 is fixed inside the culture medium bottle 1, and the bottom placement ring 41 is fixed to the bottom of the bottle, ensuring the stable position of the microporous membrane 42 and the colorimetric gas 43. The microporous membrane 42 is made of polymethyl methacrylate material. The medium possesses high transparency and chemical stability, allowing for clear observation of color changes and preventing interference from other chemicals in the culture medium. Then, the color-developing gas 43, acting as hydrogen sulfide, contacts the microporous membrane 42 during SRB metabolism. When SRB bacteria produce hydrogen sulfide gas through their metabolic activity, the gas rapidly permeates into the color-developing gas 43 via the porous structure of the microporous membrane 42, reacting with it. Since the contact area between the microporous membrane 42 and the color-developing gas 43 is greater than 50%, this structural design significantly accelerates the reaction rate, thereby improving the sensitivity of the color development reaction. Ultimately, the reaction between hydrogen sulfide gas and the color-developing gas 43 triggers a noticeable color change, enabling researchers to quickly and accurately detect the metabolic status of SRB.

[0036] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments for application in other fields. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present utility model without departing from the technical solution of the present utility model shall still fall within the protection scope of the technical solution of the present utility model.

Claims

1. A SRB culture medium with hydrogen sulfide gas color developing indication, characterized in that, Comprise; Culture medium bottle (1); Fast detection assembly (4); the fast detection assembly (4) includes the bottom retaining ring (41) fixedly connected in the inside bottom end of culture medium bottle (1), the top end of the bottom retaining ring (41) is fixedly connected with microporous membrane (42), and the bottom retaining ring (41) is provided with color developing gas (43) between microporous membrane (42), and the color developing gas (43) is hydrogen sulfide gas.

2. The SRB culture medium with hydrogen sulfide gas color developing indication according to claim 1, characterized in that: The outside top end of the culture medium bottle (1) is provided with a threaded groove, and the top end of the culture medium bottle (1) is threadedly connected with a sealing cover (2) through the threaded groove.

3. The SRB culture medium with hydrogen sulfide gas color developing indication according to claim 1, characterized in that: The outside of the culture medium bottle (1) is fixedly connected with an observation outer plate (3).

4. The SRB culture medium with hydrogen sulfide gas color developing indication according to claim 1, characterized in that: The microporous membrane (42) is made of polymethyl methacrylate material.

5. The SRB culture medium with hydrogen sulfide gas color developing indication according to claim 1, characterized in that: The contact area between the color developing gas (43) and the microporous membrane (42) is greater than 50% of the surface area of the microporous membrane (42).

Images