Microorganism separation purifier

Abstract

The utility model relates to the technical field of microorganism purification, in particular to a microorganism separation and purification device which is convenient to disassemble and assemble, simple in structure and high in use performance when a biological membrane needs to be replaced or cleaned. Comprising a first box body, a second box body, a plurality of biological membranes, a feeding assembly, a connecting assembly and a control assembly, the second box body is placed and installed above the first box body through a connecting column, the feeding assembly is installed at the top of the second box body, the connecting assembly is installed between the first box body and the second box body, and the biological membranes are installed on the connecting assembly; a control assembly is installed at the bottom of the first box.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of microbial purification, particularly to a microbial separation and purification device. BACKGROUND

[0002] In biology, microbiology research and use, in order to prevent and treat miscellaneous bacteria interference, often need high purity microbial cells. The conventional microbial cell purification is all in the petri dish using selective medium repeatedly implements multiple separation, and this conventional purification method has the defect of long purification time, brings inconvenience to the experiment of microbial cells, thereby reduces the efficiency of purification experiment, and the prior art announcement No. CN208632536U proposes a microbial cell purification device, including first culture bottle and second culture bottle, the top of first culture bottle is connected with liquid inlet pipe, the top of second culture bottle is filled with sealing pad, the top of sealing pad is provided with injection port, the surface of second culture bottle is wound with coil, the input end of coil is electrically connected with radio frequency instrument, the top and bottom of left side of first culture bottle are both connected with first glass tube. But the pipeline with biological membrane is more complex to disassemble, when the biological membrane in the pipeline is damaged or needs cleaning, it cannot be disassembled and assembled conveniently. SUMMARY

[0003] To solve the above technical problems, the utility model provides a microbial separation and purification device, which is convenient to disassemble and assemble when the biological membrane needs to be replaced or cleaned, has simple structure and high use performance.

[0004] The microbial separation and purification device comprises a first box body, a second box body, a plurality of biological membranes, a feeding assembly, a connecting assembly and a control assembly. The second box body is placed and installed above the first box body through a connecting column. The feeding assembly is installed on the top of the second box body. The connecting assembly is installed between the first box body and the second box body. The biological membranes are installed on the connecting assembly. The control assembly is installed at the bottom of the first box body. The culture solution or cell factor can be extracted through the feeding assembly, and then the culture solution is injected into the second box body, thereby improving the feeding efficiency. The connecting assembly connects the first box body and the second box body. The biological membranes allow the cell factor to pass through but do not allow the cells to pass through. Different cell factors can be injected into the first box body according to the cell growth condition, so as to promote the growth of the cells to be purified and inhibit the growth of other cells, thereby achieving the purpose of purifying the cells. The biological membranes are installed on the connecting assembly, so that the biological membranes can be conveniently disassembled and assembled when they need to be replaced or cleaned. The control assembly can effectively control the equipment. The device has simple structure and high use performance.

[0005] Preferably, the connecting assembly comprises a plurality of connecting pipes, a plurality of valves, a plurality of mounting plates, a plurality of sealing plates and a plurality of connecting flanges, the input ends of the plurality of connecting pipes are uniformly arranged and mounted at the lower end of the right side wall of the second box body, the valves are mounted on the connecting pipes, two mounting plates are mounted on the inner wall of the middle part of the connecting pipes, the biofilm is inserted between the two mounting plates, the sealing plate is fixedly mounted on the right side wall of the biofilm, the sealing groove is formed in the right side wall of the connecting pipe, the sealing plate is mounted in the sealing groove through screws, the through hole matched with the connecting pipe is formed in the upper part of the right side wall of the first box body, the connecting flange is mounted at the output end of the connecting pipe, and the connecting flange is sealingly connected with the through hole of the right side wall of the first box body through screws; the plurality of valves are opened, the biofilm allows the cytokines to pass through but cannot allow the cells to pass through, different cytokines can be injected into the first box body according to the growth of the cells, the growth of the cells to be purified is promoted, and the growth of other cells is inhibited, so that the purpose of purifying the cells is achieved, the screws on the sealing plate are unscrewed, the sealing plate is pulled to take out the biofilm in the connecting pipe, and when the connecting pipe needs to be replaced or cleaned, the connecting pipe is convenient to disassemble and assemble.

[0006] Preferably, the feeding assembly comprises a feeding cylinder, a feeding pipe, a first one-way valve, a feeding pipe, a second one-way valve, a mounting table, an electric telescopic rod and a piston plate, the feeding cylinder is mounted at the left end of the top of the second box body, the feeding pipe is connected to the left end of the top of the feeding cylinder, the first one-way valve is mounted on the feeding pipe, the feeding pipe is connected to the left side output end of the feeding cylinder, the output end of the feeding pipe is communicated with the second box body, the second one-way valve is mounted on the feeding pipe, the mounting table is mounted at the top of the second box body, the telescopic end of the electric telescopic rod is provided with a pull rod, the pull rod slidably penetrates the right end of the feeding cylinder, the piston plate is mounted on the left end of the pull rod, and the piston plate is sealingly and slidably mounted in the feeding cylinder; the culture solution is connected to the input end of the feeding pipe, the first one-way valve is opened, then the electric telescopic rod is started to pull the pull rod to pull the piston plate to move in the feeding cylinder, so that the culture solution or the cytokine can be extracted, then the second one-way valve is opened, the electric telescopic rod is started to push the pull rod to drive the piston plate to move, so that the culture solution can be injected into the second box body to complete feeding and improve work efficiency.

[0007] Preferably, the feeding assembly comprises a feeding cylinder, a feeding pipe, a first one-way valve, a feeding pipe, a second one-way valve, a mounting table, an electric telescopic rod and a piston plate, the feeding cylinder is mounted at the left end of the top of the second box body, the feeding pipe is connected to the left end of the top of the feeding cylinder, the first one-way valve is mounted on the feeding pipe, the feeding pipe is connected to the left side output end of the feeding cylinder, the output end of the feeding pipe is communicated with the second box body, the second one-way valve is mounted on the feeding pipe, the mounting table is mounted at the top of the second box body, the telescopic end of the electric telescopic rod is provided with a pull rod, the pull rod slidably penetrates the right end of the feeding cylinder, the piston plate is mounted on the left end of the pull rod, and the piston plate is sealingly and slidably mounted in the feeding cylinder; the culture solution is connected to the input end of the feeding pipe, the first one-way valve is opened, then the electric telescopic rod is started to pull the pull rod to pull the piston plate to move in the feeding cylinder, so that the culture solution or the cytokine can be extracted, then the second one-way valve is opened, the electric telescopic rod is started to push the pull rod to drive the piston plate to move, so that the culture solution can be injected into the second box body to complete feeding and improve work efficiency.

[0008] Preferably, it also includes a first heater and a second heater. Heating chambers are provided at the bottom of the first and second boxes. The first heater is installed in the heating chamber at the bottom of the second box, and the second heater is installed in the heating chamber at the bottom of the first box. The first heater and the second heater can heat the heating chamber when the purified cells need to be purified at a specific temperature, so as to meet the purpose of the purified cells needing a specific temperature environment to be purified.

[0009] Preferably, the control assembly includes a controller, a heating controller, a temperature detector, a display panel, and multiple friction plates. The controller is installed at the bottom of the first housing, and friction plates are installed at the four corners of the bottom of the controller. The heating controller, temperature detector, and display panel are installed on the controller. The heating controller is electrically connected to the first heater and the second heater. The detection end of the temperature detector is located inside the two heating chambers. The controller facilitates the control of the equipment, the friction plates increase friction and ensure stability, and the heating controller and temperature detector can effectively dynamically adjust the temperature to achieve the purpose of dynamic temperature regulation.

[0010] Compared with the prior art, the beneficial effects of this utility model are as follows: the feeding component can extract the culture medium or cytokines, and then inject the culture medium into the second chamber, improving the feeding efficiency; the connecting component connects the first chamber and the second chamber; the biomembrane allows cytokines to pass through but not cells; different cytokines can be injected into the first chamber according to the cell growth status to promote the growth of the cells to be purified and inhibit the growth of other cells, thereby achieving the purpose of cell purification; the biomembrane is installed on the connecting component, making it easy to disassemble and assemble when the biomembrane needs to be replaced or cleaned; the control component can effectively control the equipment; the structure is simple and the performance is high. Attached Figure Description

[0011] Figure 1 This is a schematic diagram of the structure of this utility model;

[0012] Figure 2 This is a schematic diagram of the isometric structure of this utility model;

[0013] Figure 3 This is a schematic diagram of the lower three-dimensional structure of this utility model;

[0014] Figure 4 This is a partial cross-sectional structural schematic diagram of the present invention;

[0015] Figure 5 This is a front cross-sectional structural diagram of the present invention;

[0016] The attached diagram is labeled as follows: 1. Box No. 1; 2. Box No. 2; 3. Connecting column; 4. Biofilm; 5. Feeding cylinder; 6. Feed pipe; 7. First check valve; 8. Feeding pipe; 9. Second check valve; 10. Mounting platform; 11. Electric telescopic rod; 12. Pull rod; 13. Piston plate; 14. Connecting pipe; 15. Valve; 16. Mounting plate; 17. Sealing plate; 18. Connecting flange; 19. Injection port; 20. Discharge pipe; 21. Controller; 22. First heater; 23. Second heater; 24. Heating controller; 25. Temperature detector; 26. Display panel; 27. Friction plate. Detailed Implementation

[0017] To facilitate understanding of this utility model, a more complete description will be given below with reference to the accompanying drawings. This utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to make the disclosure of this utility model more thorough and complete.

[0018] like Figures 1 to 5As shown, the second chamber 2 is placed above the first chamber 1 via a connecting column 3. The input ends of multiple connecting pipes 14 are evenly arranged and installed on the lower right side wall of the second chamber 2. Valves 15 are installed on the connecting pipes 14. Two mounting plates 16 are installed on the inner wall of the middle section of the connecting pipes 14. The biofilm 4 is inserted between the two mounting plates 16. A sealing plate 17 is fixedly installed on the right side wall of the biofilm 4. A sealing groove is formed on the right side wall of the connecting pipes 14, and the sealing plate 17 is installed in the sealing groove by screws. A through hole matching the connecting pipe 14 is provided on the upper right side wall of the housing 1. A connecting flange 18 is installed at the output end of the connecting pipe 14. The connecting flange 18 is sealed to the through hole on the right side wall of the housing 1 by screws. The feeding cylinder 5 is installed at the top left end of the housing 2. The top left end of the feeding cylinder 5 is connected to the feeding pipe 6. A first one-way valve 7 is installed on the feeding pipe 6. The left output end of the feeding cylinder 5 is connected to the feeding pipe 8. The output end of the feeding pipe 8 is connected to the housing 2. A second one-way valve 7 is installed on the feeding pipe 8. A mounting platform 10 is installed at the bottom of the valve 9 and the electric telescopic rod 11. The mounting platform 10 is installed on the top of the second box 2. A pull rod 12 is provided at the telescopic end of the electric telescopic rod 11. The pull rod 12 slides through the right end of the feeding cylinder 5. A piston plate 13 is installed at the left end of the pull rod 12. The piston plate 13 is sealed and slidably installed inside the feeding cylinder 5. The injection port 19 is connected to the upper part of the left side wall of the first box 1. The lower part of the right side wall of the first box 1 is connected to the discharge pipe 20. Heating chambers are opened at the bottom of the first box 1 and the second box 2. The first heater 22 is installed in the heating chamber at the bottom of the second box 2. The second heater 23 is installed in the heating chamber at the bottom of the first box 1. The controller 21 is installed at the bottom of the first box 1. Friction plates 27 are installed at the four corners of the bottom of the controller 21. A heating controller 24, a temperature detector 25 and a display panel 26 are installed on the controller 21. The heating controller 24 is electrically connected to the first heater 22 and the second heater 23. The detection end of the temperature detector 25 is located in the two heating chambers.

[0019] Clean and disinfect all parts. Place the second chamber 2 on top of the first chamber 1. Seal the connecting flange 18 to the through hole on the right side wall of the first chamber 1 with screws. Connect the culture medium to the input end of the feed pipe 6. Open the first one-way valve 7. Then, activate the electric telescopic rod 11 to pull the lever 12, which moves the piston plate 13 inside the feeding cylinder 5. This allows for the extraction of culture medium or cytokines. Then, open the second one-way valve 9. Activate the electric telescopic rod 11 to push the lever 12, which in turn moves the piston plate 13, allowing the culture medium to be injected into the second chamber 2, completing the feeding process and improving work efficiency. Open multiple sets of valves 15. The biomembrane 4 allows cytokines to pass through but not cells. Different cytokines can be injected into the first chamber 1 according to the cell growth status to promote the growth of the cells to be purified and inhibit the growth of other cells, thereby achieving the purpose of cell purification. Unscrew the screws on the sealing plate 17. Pulling the sealing plate 17 removes the biofilm 4 from the connecting tube 14, making it easier to disassemble and clean the connecting tube 14 when needed. Cytokines are injected into the first chamber 1 through the injection tube and injection port 19. Different cytokines can be injected into the first chamber 1 according to the cell growth status to promote the growth of the cells to be purified and inhibit the growth of other cells, thereby achieving the purpose of cell purification. The material is discharged through the discharge pipe 20. The first heater 22 and the second heater 23 can heat the heating chamber when the purified cells need to be purified at a specific temperature to meet the purpose of purifying cells by requiring a specific temperature environment. The controller 21 facilitates the control of the equipment. The friction plate 27 can increase friction and ensure stability. The heating controller 24 and the temperature detector 25 can effectively dynamically adjust the temperature to achieve the purpose of dynamic temperature regulation.

[0020] like Figures 1 to 5As shown, this utility model discloses a microbial isolation and purification device. During operation, all parts are cleaned and disinfected. The second chamber 2 is placed on top of the first chamber 1. The connecting flange 18 is sealed to the through-hole on the right side wall of the first chamber 1 using screws. The culture medium is connected to the input end of the feed pipe 6. The first one-way valve 7 is opened, and then the electric telescopic rod 11 is activated, pulling the pull rod 12 to move the piston plate 13 within the feeding cylinder 5, thereby extracting the culture medium or cytokines. Then, the second one-way valve 9 is opened, and the electric telescopic rod 11 is activated again, pushing the pull rod 12 to move the piston plate 13, thus injecting the culture medium into the second chamber 2. Multiple valves 15 are opened, and the biofilm 4 allows cytokines to pass through but not cells. Different types of cells can be injected into the first chamber 1 according to the cell growth status. Cytokines promote the growth of cells to be purified and inhibit the growth of other cells, thereby achieving the purpose of cell purification. Cytokines are injected into the first chamber 1 through the injection tube and injection port 19. Different cytokines can be injected into the first chamber 1 according to the cell growth status to promote the growth of cells to be purified and inhibit the growth of other cells. Unscrew the screw on the sealing plate 17 and pull the sealing plate 17 to remove the biofilm 4 in the connecting tube 14, so that the connecting tube 14 is easy to disassemble and assemble when it needs to be replaced or cleaned. The first heater 22 and the second heater 23 can heat the heating chamber when the purified cells need to be purified at a specific temperature. The controller 21 facilitates the control of the equipment. The temperature can be effectively dynamically adjusted through the heating controller 24 and the temperature detector 25.

[0021] The biofilm 4, controller 21, heating controller 24, temperature detector 25, and display panel 26 of this microbial separation and purification device are commercially available. Technical personnel in this industry only need to install and operate them according to the accompanying instruction manual, without requiring any creative work from those skilled in the art.

[0022] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A microbial isolation purifier, characterized in that, The utility model provides a kind of biological membrane reactor, including box (1), box (2), multiple biological membranes (4), feeding assembly, connecting assembly and control assembly, box (2) is placed and installed above box (1) by connecting column (3), feeding assembly is installed on the top of box (2), connecting assembly is installed between box (1) and box (2), biological membrane (4) is installed on connecting assembly, control assembly is installed at the bottom of box (1).

2. A microbe isolation purifier as claimed in claim 1, wherein, Connecting assembly includes multiple connecting pipes (14), multiple valves (15), multiple mounting plates (16), multiple sealing plates (17) and multiple connecting flanges (18), the input end of multiple connecting pipes (14) is evenly arranged and installed at the lower end of the right side wall of box (2), valve (15) is installed on connecting pipe (14), two mounting plates (16) are installed on the inner wall of the middle part of connecting pipe (14), biological membrane (4) is inserted and mounted between two mounting plates (16), sealing plate (17) is fixedly installed on the right side wall of biological membrane (4), sealing groove is formed on the right side wall of connecting pipe (14), sealing plate (17) is installed in sealing groove by screw, the upper part of the right side wall of box (1) is provided with through hole matched with connecting pipe (14), connecting flange (18) is installed on the output end of connecting pipe (14), and connecting flange (18) is sealingly connected with the through hole of the right side wall of box (1) by screw.

3. A microbe isolation and purifier as claimed in claim 1, wherein, Feeding assembly includes feeding cylinder (5), feed pipe (6), first one-way valve (7), feeding pipe (8), second one-way valve (9), mounting table (10), electric telescopic rod (11) and piston plate (13), feeding cylinder (5) is installed at the top left end of box (2), feeding cylinder (5) is connected with feed pipe (6) at the top left end, first one-way valve (7) is installed on feed pipe (6), feeding pipe (8) is connected with the left output end of feeding cylinder (5), the output end of feeding pipe (8) is communicated with box (2), second one-way valve (9) is installed on feeding pipe (8), mounting table (10) is installed at the bottom of electric telescopic rod (11), mounting table (10) is installed at the top of box (2), pull rod (12) is arranged at the telescopic end of electric telescopic rod (11), pull rod (12) is slidably penetrated through the right end of feeding cylinder (5), piston plate (13) is installed on the left end of pull rod (12), and piston plate (13) is sealingly and slidably installed in feeding cylinder (5).

4. A micro-organism separator-purifier as claimed in claim 1, wherein, It also includes injection port (19) and discharge pipe (20), injection port (19) is connected on the upper part of the left side wall of box (1), and discharge pipe (20) is connected on the lower part of the right side wall of box (1).

5. A micro-organism separator-purifier as claimed in claim 1, wherein, It also includes first heater (22) and second heater (23), heating cavities are formed in the bottom of box (1) and box (2), first heater (22) is installed in the heating cavity in the bottom of box (2), and second heater (23) is installed in the heating cavity in the bottom of box (1).

6. A micro-organism separator-purifier as claimed in claim 5, wherein, The control assembly comprises a controller (21), a heating controller (24), a temperature detector (25), a display panel (26) and a plurality of friction plates (27), the controller (21) is installed at the bottom of the first box body (1), the friction plates (27) are respectively installed at the four corners of the bottom of the controller (21), the heating controller (24), the temperature detector (25) and the display panel (26) are installed on the controller (21), the heating controller (24) is electrically connected with the first heater (22) and the second heater (23), and the detection end of the temperature detector (25) is located in the two heating cavities.

Images