Serum-free culture device for improving exosome secretion of stem cells

Abstract

The utility model relates to the technical field of stem cell exosome culture, and discloses a serum-free culture device for improving stem cell exosome secretion, which comprises a protective box, a base and a culture dish, the base is fixedly connected with the bottom of the protective box, the bottom of the culture dish is fixedly provided with a mounting sleeve, and the mounting sleeve is fixedly connected with the protective box. The culture dish is located in the protection box, a protruding block is fixedly installed in the center of the interior of the base, a second telescopic rod is fixedly arranged in a through groove, a clamping block is fixedly arranged at the other end of the second telescopic rod, and a second spring is connected to the second telescopic rod in a sleeved mode. The elasticity of four groups of springs I is utilized to respectively drive long telescopic pipes on four groups of telescopic rods I to move, then four groups of springs II are utilized to drive telescopic rods II to push clamping blocks to abut against the outer wall of a mounting sleeve, and meanwhile, a buffering effect is provided for the culture dish, so that secretion of stem cell exosomes stored in the culture dish is facilitated.

Description

Technical Field

[0001] This utility model relates to the field of stem cell exosome culture technology, and in particular to a serum-free culture device for improving stem cell exosome secretion. Background Technology

[0002] Exosomes are a type of cell secretion from stem cells and are an important medium for intercellular communication. They have the ability to cross the blood-brain barrier. Stem cell exosomes have strong repair, regeneration, and protective effects in various types of damage repair. Exosomes can be stored for a long time at -80°C or in liquid nitrogen. Low temperature can effectively inhibit biological reactions and degradation processes. Serum-free culture refers to the method of culturing animal cells using a serum-free culture medium.

[0003] An existing high-efficiency culture dish for stem cell exosomes (publication number: CN217230769U) has a structural design that allows the modular culture dish box components to be assembled in different ways, enabling the simultaneous culture of multiple samples. However, the stability of the culture dish is not ideal during handling and use. The culture dish is easily shaken and bumped, which is not conducive to promoting the division of stem cell exosomes stored inside the culture dish. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing a serum-free culture device for improving stem cell exosome secretion.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A serum-free culture device for enhancing stem cell exosome secretion includes a protective box, a base, and a culture dish. The base is fixedly connected to the bottom of the protective box. An installation sleeve is fixedly installed on the bottom of the culture dish, which is located inside the protective box. A protrusion is fixedly installed at the center of the base. A positioning post is fixedly installed at the bottom end of the installation sleeve, and the positioning post is embedded in and connected to the protrusion. A telescopic rod is fixedly installed on the side wall of the protrusion. A fixing block is fixedly installed at the other end of the telescopic rod. A spring is sleeved on the telescopic rod. A through groove is formed in the inner wall of the base. A second telescopic rod is fixedly installed in the through groove. A locking block is fixedly installed at the other end of the second telescopic rod. A spring is sleeved on the second telescopic rod, and the locking block is embedded in and connected to the inner wall of the installation sleeve.

[0007] As a further embodiment of this utility model, the two ends of the first spring are respectively fixedly connected to the protrusion and the fixing block, and the two ends of the second spring are respectively fixedly connected to the inner wall of the through groove and the locking block.

[0008] As a further embodiment of this utility model, a limiting groove is provided on the inner wall of the base, and the telescopic rod is located above the limiting groove.

[0009] As a further embodiment of this utility model, the fixing block is slidably connected to the limiting groove, the fixing block is evenly distributed on the protrusion, and the top of the culture dish is provided with a dish lid.

[0010] As a further embodiment of this utility model, the dish cover is embedded and connected to the culture dish, the locking blocks are evenly distributed inside the base, and a support column is fixedly provided on the outer wall of the base.

[0011] As a further embodiment of this utility model, the top of the protective box is fitted with a sealing cover via a hinge, and a rubber pad is fixedly provided at the bottom of the support column, with the rubber pads being evenly distributed on the base.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] In use, staff first prepare the culture dish containing the stem cell exosome sample, place the dish inside the protective box, and push the positioning column to engage with the protrusion. Utilizing the elasticity of four sets of springs, the long telescopic tubes on the four sets of telescopic rods move, thereby pushing the four sets of fixing blocks against the inner wall of the mounting sleeve. Then, through the four sets of springs, the telescopic rods push the locking blocks against the outer wall of the mounting sleeve. With the cooperation of springs, telescopic rods, springs, and telescopic rods, the culture dish is fixed inside the protective box. As the protective box moves the culture dish, it also provides a buffer, reducing shaking and enhancing the sealing and protection of the culture dish, keeping it stable. This, in turn, promotes the secretion of stem cell exosomes stored inside the culture dish. Attached Figure Description

[0014] Figure 1 This is a schematic cross-sectional view of a serum-free culture device for improving stem cell exosome secretion proposed in this utility model.

[0015] Figure 2 This is a three-dimensional structural schematic diagram of a serum-free culture device for improving stem cell exosome secretion proposed in this utility model;

[0016] Figure 3 This is an enlarged structural diagram of point A of a serum-free culture device for improving stem cell exosome secretion proposed in this utility model;

[0017] Figure 4 This is a schematic diagram of the disassembled structure of the mounting sleeve of a serum-free culture device for improving stem cell exosome secretion proposed in this utility model;

[0018] In the diagram: 1. Protective box; 101. Sealing cover; 102. Spring 1; 103. Telescopic rod 1; 104. Fixing block; 105. Limiting groove; 106. Through groove; 2. Base; 201. Support column; 202. Rubber pad; 203. Positioning column; 3. Petri dish; 301. Dish lid; 302. Mounting sleeve; 303. Protrusion; 4. Telescopic rod 2; 5. Spring 2; 6. Locking block. Detailed Implementation

[0019] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0020] In the description of this utility model, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," and "the other end," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0021] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," and "connected," etc., should be interpreted broadly. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0022] Reference Figures 1-4 A serum-free culture device for enhancing stem cell exosome secretion includes a protective box 1, a base 2, and a culture dish 3. The base 2 is fixedly connected to the bottom of the protective box 1. An installation sleeve 302 is fixedly installed at the bottom of the culture dish 3, which is located inside the protective box 1. A protrusion 303 is fixedly installed at the center of the base 2. A positioning post 203 is fixedly installed at the bottom end of the installation sleeve 302, which is embedded in the protrusion 303. A telescopic rod 103 is fixedly installed on the side wall of the protrusion 303. A fixing block 104 is fixedly installed at the other end of the telescopic rod 103. A spring 102 is sleeved on the telescopic rod 103. A through groove 106 is opened in the inner wall of the base 2. A telescopic rod 2 4 is fixedly installed in the through groove 106. A locking block 6 is fixedly installed at the other end of the telescopic rod 2 4. A spring 2 5 is sleeved on the telescopic rod 2 4. The locking block 6 is embedded in the inner wall of the installation sleeve 302.

[0023] In use, the staff first prepares the culture dish 3 containing the stem cell exosome sample, places the culture dish 3 into the protective box 1, pushes the positioning column 203 to engage with the protrusion 303, and uses the elasticity of the four sets of springs 102 to drive the long telescopic tubes on the four sets of telescopic rods 103 to move, thereby pushing the four sets of fixing blocks 104 to abut against the inner wall of the mounting sleeve 302. Then, through the four sets of springs 25, the telescopic rods 24 push the locking block 6 to abut against the outer wall of the mounting sleeve 302. With the cooperation of springs 102, telescopic rods 103, springs 25 and telescopic rods 24, the culture dish 3 is fixed inside the protective box 1. When the protective box 1 moves the culture dish 3, it also provides a buffer for the culture dish 3, reducing shaking and strengthening the sealing and protection effect of the culture dish 3, so that the culture dish 3 can remain stable, which is conducive to promoting the secretion of stem cell exosomes stored inside the culture dish 3.

[0024] In this embodiment, the two ends of spring 102 are fixedly connected to protrusion 303 and fixing block 104 respectively, and the two ends of spring 25 are fixedly connected to the inner wall of through groove 106 and locking block 6 respectively.

[0025] In use, four sets of spring 102, telescopic rod 103 and fixing block 104 are provided on the protrusion 303. By pushing the four sets of fixing blocks 104 to fit tightly against the inner wall of the mounting sleeve 302, the initial positioning is performed.

[0026] In this embodiment, a limiting groove 105 is provided on the inner wall of the base 2, and the telescopic rod 103 is located above the limiting groove 105.

[0027] In use, four sets of spring 25 and telescopic rod 24 are installed inside the base 2, and the support column 201 provides support, while the rubber pad 202 increases the stability of placement.

[0028] In this embodiment, the fixing block 104 is slidably connected to the limiting groove 105, the fixing block 104 is evenly distributed on the protrusion 303, and the top of the culture dish 3 is provided with a dish cover 301.

[0029] In use, the limiting groove 105 provides a limiting function for the fixing block 104. Under the action of the spring 102 and the telescopic rod 103, the fixing block 104 is pushed to slide along the inner wall of the limiting groove 105, thereby closely adhering to the inner wall of the mounting sleeve 302.

[0030] In this embodiment, the dish cover 301 is embedded in the connected culture dish 3, the locking blocks 6 are evenly distributed inside the base 2, and the outer wall of the base 2 is fixedly provided with support columns 201.

[0031] In use, four sets of locking blocks 6 are arranged inside the base 2. By making the four sets of locking blocks 6 fit tightly against the outer wall of the mounting sleeve 302 and cooperate with the four sets of fixing blocks 104, the culture dish 3 is fixed inside the base 2.

[0032] In this embodiment, a sealing cover 101 is installed on the top of the protective box 1 via a hinge, and a rubber pad 202 is fixedly installed at the bottom of the support column 201. The rubber pads 202 are evenly distributed on the base 2.

[0033] When in use, the protective box 1 and the sealing cover 101 work together to enhance the sealing effect on the culture dish 3, reduce the interference of external factors on the culture dish 3, and thus facilitate the division of stem cell exosomes in the culture dish 3.

[0034] From the above description, it can be seen that the above-described embodiments of this utility model achieve the following technical effects: In use, the staff first prepares the culture dish 3 of the stem cell exosome sample, puts the culture dish 3 into the protective box 1, pushes the positioning column 203 to insert with the protrusion 303, and uses the elasticity of the four sets of springs 102 to drive the long telescopic tubes on the four sets of telescopic rods 103 to move, thereby pushing the four sets of fixing blocks 104 to abut against the inner wall of the mounting sleeve 302. Again, through the four sets of springs 25, the telescopic rods 24 push the locking block 6 to abut against the outer wall of the mounting sleeve 302. With the cooperation of springs 102, telescopic rods 103, springs 25 and telescopic rods 24, the culture dish 3 is fixed inside the protective box 1. When the protective box 1 moves the culture dish 3, it also provides a buffer for the culture dish 3, reduces shaking, strengthens the sealing and protection effect of the culture dish 3, and enables the culture dish 3 to remain stable, which is conducive to promoting the secretion of stem cell exosomes stored inside the culture dish 3.

[0035] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A serum-free culture device for improving the exosome secretion of stem cells, comprising a protective box (1), a base (2) and a culture dish (3), characterized in that: The base (2) is fixedly connected to the bottom of the protective box (1), the bottom of the culture dish (3) is fixedly provided with a mounting sleeve (302), the culture dish (3) is located in the interior of the protective box (1), the center position in the interior of the base (2) is fixedly provided with a protruding block (303), the bottom end of the mounting sleeve (302) is fixedly provided with a positioning column (203), the positioning column (203) is embeddedly connected to the protruding block (303), the sidewall of the protruding block (303) is fixedly provided with a telescopic rod one (103), the other end of the telescopic rod one (103) is fixedly provided with a fixed block (104), the telescopic rod one (103) is sleeved with a spring one (102), the inner wall of the base (2) is provided with a through slot (106), the through slot (106) is fixedly provided with a telescopic rod two (4), the other end of the telescopic rod two (4) is fixedly provided with a clamping block (6), the telescopic rod two (4) is sleeved with a spring two (5), and the clamping block (6) is embeddedly connected to the inner wall of the mounting sleeve (302).

2. The serum-free culture device for improving the exosome secretion of stem cells according to claim 1, characterized by, The two ends of the spring one (102) are fixedly connected to the protruding block (303) and the fixed block (104) respectively, and the two ends of the spring two (5) are fixedly connected to the inner wall of the through slot (106) and the clamping block (6) respectively. 3.The serum-free culture device for improving the exosome secretion of stem cells according to claim 1, wherein, The inner wall of the base (2) is provided with a limiting slot (105), and the telescopic rod one (103) is located above the limiting slot (105).

4. The serum-free culture device for improving the exosome secretion of stem cells according to claim 3, characterized by, The fixed block (104) is slidably connected to the limiting slot (105), and the fixed block (104) is equidistantly distributed on the protruding block (303), and the top of the culture dish (3) is provided with a dish cover (301).

5. The serum-free culture device for improving the exosome secretion of stem cells according to claim 4, characterized by, The dish cover (301) is embeddedly connected to the culture dish (3), the clamping block (6) is equidistantly distributed in the interior of the base (2), and the outer wall of the base (2) is fixedly provided with a supporting column (201).

6. The serum-free culture device for enhancing the exosome secretion of stem cells according to claim 5, wherein The top of the protective box (1) is provided with a sealing cover (101) through a hinge, the bottom end of the supporting column (201) is fixedly provided with a rubber pad (202), and the rubber pad (202) is equidistantly distributed on the base (2).

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