Assembled cell incubation device for antibody production

Abstract

The utility model discloses a split mounting type cell incubation device for antibody production, which comprises a base, the base is of a rectangular frame body structure, the upper end of the base is connected with a heat preservation cover in a clamping manner, an incubation frame is placed at the upper end of the base in the heat preservation cover, and the incubation frame provides a main supporting structure for a cell incubation tube. According to the split mounting type cell incubation device for antibody production, the heating plates and the magnetic clamping blocks mounted in the base are of a split mounting type structure and are flexibly combined according to different experiment requirements during use, part of split mounting modules are used for small-scale cell incubation experiments, and energy consumption and equipment occupied space are reduced; under the condition of simultaneous incubation of large-scale or multi-variety cells, the number of the heating plates and the magnetic clamping blocks can be increased or the splicing layout between the heating plates and the magnetic clamping blocks can be adjusted, connection is performed by increasing the number of the heating plates, and setting of different temperature areas of the incubation frame in the base is realized by adjusting the temperature of each heating plate.

Description

Technical Field

[0001] This utility model relates to the field of biological antibody production equipment technology, specifically a modular cell incubation device for antibody production. Background Technology

[0002] Antibodies are immunoglobulins produced by the body in response to antigen stimulation, providing protection. They are large, structurally specific proteins secreted by plasma cells. As an important component of the immune system, they can precisely identify and effectively neutralize invading foreign substances such as bacteria and viruses. Antibodies recognize a unique characteristic of a specific foreign substance and produce an immune response product; this foreign target is called an antigen.

[0003] In modern biomedical research and biotechnology, the large-scale cultivation of engineered cell lines using suitable bioreactors to produce useful metabolites such as antibodies or to enable them to exert their unique physiological functions is an emerging technology. Cell incubation, as a fundamental technique in this field, allows cells to rapidly proliferate in a suitable environment through precise control of culture conditions such as temperature, humidity, and gas, which helps to better understand the biological characteristics of cells.

[0004] Temperature is a critical factor in cell incubation; precise and stable temperature control plays a decisive role in cell growth, proliferation, metabolic activity, and the maintenance of cell viability. Traditional incubation devices often employ a monolithic heating method, with heating wires installed inside the incubator walls or at the bottom to raise the overall temperature. Since the heating components are fixed inside the device, if critical components such as the heating wires or temperature sensors fail, repairs require disassembling a large amount of the outer casing and internal components, resulting in high costs and time consumption, disrupting the experimental process. Furthermore, due to the fixed installation of the heating equipment, monolithic heating incubation devices struggle to provide the necessary temperature adjustments for incubation operations of varying scales, impacting the effectiveness of cell experiments. Utility Model Content

[0005] The purpose of this invention is to provide a modular cell incubation device for antibody production, so as to solve the problems and defects of traditional incubation devices mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a modular cell incubation device for antibody production, comprising a base, which is configured as a rectangular frame structure, and an insulation cover is snapped onto the upper end of the base, and an incubation rack is placed inside the insulation cover at the upper end of the base, the incubation rack providing the main support structure for the cell incubation tube.

[0007] The upper surface of the base is provided with a strip-shaped protrusion structure, and the strip-shaped protrusion structure of the base is engaged with the bottom end of the heat preservation cover. The surface of the base is covered with a metal material, and the surface of the base is attracted to the magnetic card block. A heating plate is installed in the upper opening of the magnetic card block.

[0008] The heating plate is arranged parallel to the side wall of the incubation rack, and the incubation rack is designed with a convex structure. The incubation rack has a through opening in the middle, and a ventilation plate is installed at each end of the through opening. An air circulation pipe is opened inside the incubation rack, and the air circulation pipe is located below the through opening of the incubation rack. Two positioning holes are provided on each side of the incubation rack, and the positioning holes are penetrated by positioning posts.

[0009] The above technical solution allows for flexible installation of the heating element inside the cell incubation tube, enabling greater overall flexibility and scalability to meet different experimental needs.

[0010] Preferably, the upper surface of the base is provided with a threaded hole, and the threaded hole of the base is threadedly connected to the bottom end of the positioning column.

[0011] The above technical solution ensures that the positioning column and the base are installed stably, preventing the positioning column from tilting during installation and providing support for the incubation rack.

[0012] Preferably, the magnetic card block has two types: one is a strip-shaped U-shaped opening, and the other is a right-angled U-shaped opening. The two types of magnetic card blocks are placed in an alternating rectangular shape on the upper surface of the base.

[0013] By adopting the above technical solution and setting two types of magnetic card blocks, the magnetic card blocks provide an installation position for the heating plate.

[0014] Preferably, the heating plates are assembled around the outside of the incubation rack, and the heating plates are connected by cables, and the cable assemblies of the heating plates are connected to external cables.

[0015] The above technical solution allows the heating plate to be installed in a position that is limited to the incubation rack, while the external cable provides control for the heating plate.

[0016] Preferably, the external cable passes through the bottom of the base, and a heat insulation pad is provided inside the base, with the heat insulation pad located below the incubation rack.

[0017] By adopting the above technical solution, the heat transfer to the outside is reduced with the installation of the heat insulation pad.

[0018] Preferably, the positioning post has two threaded sections on its surface, one in the middle and the other at the bottom. The thread at the bottom of the positioning post is connected to the threaded hole in the base, and the threaded section in the middle of the positioning post is connected to the positioning ring.

[0019] Using the above technical solution, the two threads on the surface of the positioning column are used to connect with the base and the positioning ring, providing different connection effects.

[0020] Preferably, the positioning post passes through two incubation racks, and the incubation racks are stacked vertically on the top of the base, with the bottom of the upper incubation rack abutting against the positioning ring.

[0021] Using the above technical solution, with the installation of two incubation racks and positioning columns, it is convenient to provide incubation for a large number of cells.

[0022] Compared with the prior art, the beneficial effects of this utility model are: This modular cell incubation device for antibody production:

[0023] 1. The heating plate and magnetic card block installed in the base are modular in structure. They can be flexibly combined according to different experimental needs. Small-scale cell incubation experiments use some modular modules to reduce energy consumption and equipment space occupation.

[0024] In cases of large-scale or simultaneous incubation of multiple cell types, the number of heating plates and magnetic card blocks can be increased or the assembly layout can be adjusted. By increasing the number of heating plates, different temperature zones can be set in the incubation rack inside the base by adjusting the temperature of each heating plate, which greatly improves the adaptability and scalability of the device and extends the service life of the equipment.

[0025] 2. Because the installation of the base, heating plate, magnetic clips, positioning columns, and incubation rack is done in an assembly manner, when a single component is damaged or a heating plate or related component malfunctions, there is no need to disassemble the entire device on a large scale as with traditional integrated incubation devices. Only the malfunctioning heating plate or individual component needs to be disassembled for repair or replacement. The operation is simple and convenient, which greatly shortens the maintenance time, reduces maintenance costs and the impact of equipment downtime on the experimental progress, and also facilitates the regular maintenance and upkeep of the equipment. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of the overall disassembled three-dimensional structure of this utility model;

[0027] Figure 2 This is a schematic diagram of the overall external three-dimensional structure of this utility model;

[0028] Figure 3 This is a side-section three-dimensional structural diagram of the internal structure of the heat insulation cover of this utility model;

[0029] Figure 4 A three-dimensional structural diagram of the incubator rack installation of this utility model;

[0030] Figure 5This is a three-dimensional structural diagram of the base and heat insulation pad of this utility model.

[0031] Figure 6 This is a three-dimensional structural diagram of the incubation rack and ventilation plate of this utility model.

[0032] In the diagram: 1. Base; 2. Insulation cover; 3. Magnetic card block; 4. Heating plate; 5. External cable; 6. Positioning post; 7. Positioning ring; 8. Incubation rack; 9. Ventilation plate; 10. Positioning hole; 11. Air circulation pipe; 12. Heat insulation pad. Detailed Implementation

[0033] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0034] Please see Figure 1-6 This utility model provides a technical solution: a modular cell incubation device for antibody production, including a base 1, a heat preservation cover 2, a magnetic card block 3, a heating plate 4, an external cable 5, a positioning column 6, a positioning ring 7, an incubation rack 8, a ventilation plate 9, a positioning hole 10, an air circulation pipe 11, and a heat insulation pad 12.

[0035] Among them, the base 1 is set as a rectangular frame structure, and the upper end of the base 1 is connected to the heat preservation cover 2. The upper end of the base 1 is placed inside the heat preservation cover 2 and the incubation rack 8 provides the main support structure for the cell incubation tube.

[0036] The upper surface of the base 1 is provided with a strip-shaped protrusion structure, and the strip-shaped protrusion structure of the base 1 is engaged with the bottom end of the heat preservation cover 2. The surface of the base 1 is covered with a metal material, and the surface of the base 1 is attracted to the magnetic card block 3. The heating plate 4 is installed in the upper opening of the magnetic card block 3. The upper surface of the base 1 is provided with a threaded hole, and the threaded hole of the base 1 is threadedly connected to the bottom end of the positioning post 6. There are two types of magnetic card blocks 3: one is a strip-shaped U-shaped opening, and the other is a right-angled U-shaped opening. The two types of magnetic card blocks 3 are placed in a rectangular shape on the upper surface of the base 1 in an alternating manner.

[0037] Referring to the attached diagrams in the instruction manual Figure 1-6As shown, during use, the base 1 and the heat preservation cover 2 are assembled. The heat preservation plate located inside the heat preservation cover 2 provides a stable environment for heat preservation. At the same time, the heat preservation cover 2 is connected to the carbon dioxide injection device through a pipe to assist the cell incubation work inside the heat preservation cover 2. During use, the position of the magnetic card block 3 needs to be adjusted according to the installation position of the heating plate 4. Since the magnetic plate at the bottom of the magnetic card block 3 is magnetically attracted to the surface of the base 1, it is easy to quickly adjust the position of the magnetic card block 3 to match the installation of the heating plate 4. Figure 3-6 As shown, the heating plate 4 surrounds the outer layer of the incubation rack 8. The size and area of ​​the heating plate 4 can be adjusted and replaced as needed. Because the heating plate 4 adopts a modular design, when a heating module or related component fails, it is not necessary to disassemble the entire device on a large scale like traditional integrated incubation devices. This makes the overall maintenance and usage more flexible. Two types of magnetic clips 3 are arranged in an alternating rectangular shape on the upper surface of the base 1. Figure 5 As shown, the opening at the upper end of the magnetic card block 3 provides an installation position for the heating plate 4, and the threaded hole at the upper end of the base 1 provides assistance for the installation of the bottom end of the positioning post 6, further stabilizing the positioning post 6.

[0038] The heating plate 4 is parallel to the side wall of the incubation rack 8, and the incubation rack 8 has a convex structure. A through opening is provided in the middle of the incubation rack 8, and a ventilation plate 9 is installed at each end of the through opening. An air circulation pipe 11 is provided inside the incubation rack 8, located below the through opening. Two positioning holes 10 are provided on each side of the incubation rack 8, and the positioning holes 10 are penetrated by positioning posts 6. The heating plate 4 is assembled around the outside of the incubation rack 8, and the heating plates 4 are connected by cables. The external cable 5 is connected to the base 1 and passes through the bottom end of the base 1. The base 1 is equipped with a heat insulation pad 12 and the heat insulation pad 12 is below the incubation rack 8. The positioning post 6 has two threaded structures on its surface, one thread in the middle and the other thread at the bottom. The thread at the bottom of the positioning post 6 is connected to the threaded hole of the base 1. The threaded section in the middle of the positioning post 6 is connected to the positioning ring 7. The positioning post 6 passes through two incubation racks 8 and the incubation racks 8 are stacked vertically on the top of the base 1. The bottom end of the upper incubation rack 8 abuts against the positioning ring 7.

[0039] Referring to the attached diagrams in the instruction manual Figure 1-6 As shown, the heating plate 4 is assembled from multiple pieces, as follows: Figure 1As shown, during use, a temperature sensing instrument can be added to the inner layer of the insulation cover 2 as needed. The heating plate 4, which is assembled with auxiliary components, provides heating at different temperatures in areas with inconsistent temperatures, thus providing a uniform incubation environment for the incubation rack 8. The cables of the heating plates 4 are interconnected, and the centralized cable assembly forms an external cable 5 that passes through the base 1 and connects to external monitoring equipment, facilitating the detection of the internal conditions. The positioning holes 10 installed at both ends of the incubation rack 8 are penetrated by positioning posts 6. Figure 3-6 As shown, the threaded section of the middle section of the positioning column 6 is connected to the positioning ring 7, which supports the upper incubation rack 8 and prevents the stacked incubation racks 8 from squeezing each other. When incubating a small number of cells, the single-layer incubation rack 8 can be lifted directly. At this time, the threaded section of the middle section of the positioning column 6 and the positioning ring 7 are positioned close to the bottom. The inner layer of the heat insulation pad 12 installed at the bottom of the base 1 is uniformly set with a microporous structure to facilitate air flow. The ventilation plates 9 installed on both sides of the through opening of the incubation rack 8 provide space for air flow. With the setting of the air flow pipe 11, the cell incubation tubes placed in the incubation rack 8 receive uniform flow of hot air, so that the temperature inside the cell incubation tubes is in a uniform state, reducing the adverse effects of temperature differences on experimental results and improving the accuracy and reliability of experimental data.

[0040] Working principle: When using this modular antibody production cell incubation device, the threaded hole of the base 1 is connected to the positioning post 6. Two incubation racks 8 are installed on the base 1 through the positioning post 6. The number of incubation racks 8 can be adjusted according to the number of incubations. When there are many incubation racks, they are stacked on the base 1. The positioning ring 7 is threadedly connected to the positioning post 6 to separate the two stacked incubation racks 8. When there are few incubation racks, the single-layer incubation rack 8 is raised in the middle by connecting the positioning post 6 and the positioning ring 7. The number and installation position of the heating plates 4 are adjusted by removing and installing the magnetic suction blocks 3 to adapt to the incubation racks 8 to heat the cell incubation tubes. The ventilation plate 9 and the air circulation pipe 11 facilitate air circulation, allowing the heat inside the base 1 to be quickly conducted and diffused, maintaining a uniform internal temperature and increasing the overall practicality.

[0041] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An assembled cell incubation device for antibody production, comprising: a base (1) arranged as a rectangular frame structure, and a heat preservation cover (2) is connected to the upper end of the base (1), and an incubation rack (8) is placed inside the heat preservation cover (2), and the incubation rack (8) provides a main support structure for cell incubation tubes; characterized in that: a strip-shaped protruding structure is arranged on the upper end surface of the base (1), and the strip-shaped protruding structure of the base (1) is connected to the bottom end of the heat preservation cover (2), the surface of the base (1) is covered with a metal material, and the surface of the base (1) is attracted to a magnetic clamping block (3), and a heating plate (4) is installed in the opening at the upper end of the magnetic clamping block (3); the heating plate (4) is arranged in parallel with the side wall surface of the incubation rack (8), and the incubation rack (8) is arranged as a convex structure, a through opening is arranged in the middle of the incubation rack (8), and two ventilation plates (9) are respectively installed at the two ends of the through opening of the incubation rack (8), an air flow pipe (11) is arranged in the incubation rack (8), and the air flow pipe (11) is below the through opening of the incubation rack (8), and two positioning holes (10) are respectively arranged on the two sides of the incubation rack (8), and the positioning holes (10) are penetrated by a positioning column (6).

2. The assembled cell incubation device for antibody production according to claim 1, characterized in that: threaded holes are arranged on the upper surface of the base (1), and the threaded holes of the base (1) are threadedly connected with the bottom end of the positioning column (6).

3. The assembled cell incubation device for antibody production according to claim 1, wherein: The magnetic clamping block (3) has two types, one is a strip-shaped U-shaped opening, and the other is a right-angle-shaped U-shaped opening, and the two types of magnetic clamping blocks (3) are arranged in a staggered manner on the upper surface of the base (1) to form a rectangular shape.

4. The assembled cell incubation device for antibody production according to claim 1, wherein: The heating plates (4) are assembled around the outside of the incubation rack (8), and the heating plates (4) are connected by cables, and the cable assembly of the heating plates (4) is in communication with an external cable (5).

5. The assembled cell incubation device for antibody production of claim 4, wherein: The external cable (5) penetrates the bottom end of the base (1), and a heat insulation pad (12) is arranged in the base (1), and the heat insulation pad (12) is below the incubation rack (8).

6. The assembled cell incubation device for antibody production of claim 1, wherein: Two threaded structures are arranged on the surface of the positioning column (6), one threaded structure is in the middle, and the other threaded structure is at the bottom end, and the threaded structure at the bottom end of the positioning column (6) is connected with the threaded hole of the base (1), and the threaded structure in the middle of the positioning column (6) is threadedly connected with a positioning ring (7).

7. The assembled cell incubation device for antibody production of claim 1, wherein: The positioning column (6) penetrates two incubation racks (8), and the incubation racks (8) are stacked in a vertical direction on the upper end of the base (1), and the bottom end of the upper incubation rack (8) abuts against the positioning ring (7).

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