Organ-like tissue paraffin embedding and fixing device
By designing a paraffin embedding and fixation device for organoid tissues, the limitations and operational complexity of existing devices have been overcome. This device provides stable support and positioning, improves the quality of organoid tissue sections and the accuracy of pathological diagnosis, and simplifies the operation process.
Patent Information
- Application Number
- CN202423293075.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2026-01-13
- Estimated Expiration
- 2034-12-31
AI Technical Summary
Existing organoid tissue embedding devices have design limitations, operational complexity, and material selection restrictions, making it difficult to meet the needs of different laboratories or pathological tissues, and may affect the embedding effect and section quality.
A paraffin embedding and fixation device for organoid tissues was designed, including an embedding box, a frame, a cavity, a wax pouring groove, and an embedding groove. It provides stable support and positioning, ensuring that the tissue block does not move during the embedding process, and guides the section thickness through the depth groove, simplifying the operation process.
It improves the integrity and section quality of organoid tissues, reduces operational complexity, ensures embedding effect and section accuracy, prevents tissue damage and deformation, and improves experimental reliability and pathological diagnosis accuracy.
Smart Images
Figure CN223796336U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the technical field of organoid sample paraffin embedding device, especially to organoid tissue paraffin embedding fixing device. BACKGROUND
[0002] During the embedding process of organoid tissues, a series of carefully designed steps (such as fixation, dehydration, transparency, wax immersion, and embedding) can well protect the integrity and three-dimensional structure of organoids. These steps ensure that organoids are not damaged during subsequent slicing and analysis.
[0003] The characteristics of organoid tissue embedding production mainly include: 1. Improve the quality of slicing: the embedded organoids are easier to slice and the quality of the slices is higher. This is because the embedding material (such as agarose or paraffin) provides stable support for slicing, making the slicing process smoother and more uniform. 2. Easy to observe and identify: the embedded organoid slices can be observed and identified in detail through microscopes and other equipment. This slicing method can clearly show the internal structure and cell arrangement of organoids, providing valuable pathological information for researchers. 3. Multi-step complex process: organoid tissue embedding production is a multi-step complex process, including fixation, dehydration, transparency, wax immersion, and embedding, etc. Each step requires strict control of conditions and operation details to ensure embedding effect and quality. 4. High technical requirements: due to the small size and complex structure of organoids, and the high requirements for embedding materials and technology, organoid tissue embedding production requires high operation level and experience of technical personnel, and technical personnel need to have solid professional knowledge and rich practical experience to ensure the smooth progress of embedding process and high quality of embedding effect.
[0004] With the continuous progress and development of science and technology, the way of organoid tissue embedding is also constantly innovating and improving. The emergence of some new embedding materials and technologies provides more choices and possibilities for organoid pathological embedding production.
[0005] However, the existing organoid tissue embedding devices still have the following technical problems: 1. Design limitations: Different laboratories or different pathological tissues may have different needs for embedding devices, but the existing embedding device design has certain limitations, which is difficult to meet the use requirements. For example: some special shape and size of tissue blocks may be difficult to get stable support and fixation on the existing embedding device. 2. Material limitations: The selection of the material of the embedding device may be limited, different materials have different physical and chemical properties, which may affect the embedding effect and the quality of subsequent sectioning. For example, some materials may react with the embedding agent, causing embedding failure or section quality decline. 3. Operation complexity: In some cases, the use of the embedding device may increase the complexity of the operation. For example: it is necessary to accurately adjust the position and angle of the tissue block to ensure that it is uniformly and tightly wrapped in the embedding agent, which requires operators to have higher skills and experience. Content of the utility model
[0006] (1) Technical problems to be solved
[0007] In view of the above problems, the organoid tissue paraffin embedding and fixing device is provided, especially a mold device for embedding in pathological section making (including dehydration, embedding, sectioning, staining), aiming to solve the problems of design limitations, complex operation and unreasonable embedding materials of the existing embedding and fixing device.
[0008] (2) Technical scheme
[0009] To solve the technical problems, the organoid tissue paraffin embedding and fixing device is provided, which comprises an embedding box, a frame is arranged around the embedding box, a cavity is arranged in the frame, a wax pouring groove is arranged between the cavities, an embedding groove is arranged on the inner side of the cavity, and a depth groove is arranged on the embedding groove.
[0010] Preferably, the cavity is a square body, and a plurality of cavities are arranged.
[0011] Preferably, a plurality of cavities are arranged at equal intervals side by side, and a plurality of cavities are arranged symmetrically relative to the embedding groove.
[0012] Preferably, the embedding groove is located at the center of symmetry of the frame, and the axis of the embedding groove and the axis of the depth groove are located on the same straight line.
[0013] Preferably, the depth groove is a multi-step gradient groove, and the depth groove is located at the center of the embedding box.
[0014] Preferably, the wax pouring groove is located between the embedding groove and a plurality of cavities, and a plurality of wax pouring grooves are arranged.
[0015] Preferably, the horizontal section of the embedding groove is a circular ring, and the embedding groove is located at the center of symmetry of the embedding box.
[0016] Preferably, a plurality of the inverted wax grooves are located on the embedding box, and the plurality of the inverted wax grooves are distributed equidistantly and cross each other.
[0017] Advantages
[0018] 1. The organoid tissue paraffin embedding fixing device comprises an embedding box, a frame is arranged around the embedding box, a plurality of cavities with the same shape and size are arranged in the frame, inverted wax grooves are arranged between the cavities, an embedding groove is arranged at the center of symmetry of the embedding box, and a depth groove is arranged in the embedding groove.
[0019] 2. The organoid tissue paraffin embedding fixing device provides a stable support platform for the organoid tissue to be embedded, ensures that the tissue block can maintain a fixed position during the embedding process, avoids movement or tilting, and thus guarantees the integrity of the organoid tissue and better supports subsequent experiments.
[0020] 3. The organoid tissue paraffin embedding fixing device, since the groove body of the embedding groove is in a spiral upward shape, a depth groove for reminding the embedding slice thickness is arranged on the embedding groove, the depth of the cut can be reminded during the slicing process, so as to avoid the loss of the organoid tissue caused by the depth being too deep during the slicing process, and the phenomenon that the organoid is not completely cut out caused by the depth being too shallow.
[0021] 4. The organoid tissue paraffin embedding and fixing device, after the tissue block is fixed through the embedding and fixing device, the hardness and toughness of the tissue block are improved, and the tissue block is beneficial to subsequent cutting into ideal slices, so that the slice quality and the accuracy and reliability of pathological diagnosis are improved. In addition, through the embedding and fixing device, the embedding treatment of the organoid tissue can help reduce the interference of the external environment (such as microorganisms or unstable factors in the air) on the organoid, improve the reliability of the experiment, and because the embedding agent itself has good biological compatibility, direct contact of the organoid tissue with pollutants in the culture medium or environment can be effectively prevented, and environmental pollution is not easy to cause. BRIEF DESCRIPTION OF DRAWINGS
[0022] Figure 1 It is a first perspective view of the organoid tissue paraffin embedding and fixing device of the utility model;
[0023] Figure 2 It is a second perspective view of the organoid tissue paraffin embedding and fixing device of the utility model;
[0024] Figure 3 It is a third perspective view of the organoid tissue paraffin embedding and fixing device of the utility model;
[0025] Figure 4 It is a fourth perspective view of the organoid tissue paraffin embedding and fixing device of the utility model;
[0026] Figure 5 It is a top view of the organoid tissue paraffin embedding and fixing device of the utility model.
[0027] Corresponding component names of various reference signs in the drawing are as follows: 1, embedding box; 2, frame; 3, cavity; 4, paraffin pouring groove; 5, depth groove; 6, embedding groove. DETAILED DESCRIPTION
[0028] The present application will be described in detail below with reference to the drawings and specific embodiments.
[0029] The embodiments of the present application will be described below through specific examples. Those skilled in the art can easily understand other advantages and effects of the present application from the content disclosed in the specification. Obviously, the described embodiments are only a part of the embodiments of the present application, not all the embodiments. The present application can also be implemented or applied by other different specific embodiments, and various modifications or changes can be made to the details in the specification without departing from the spirit of the present application. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.
[0030] It should be noted that various aspects of embodiments within the scope of the appended claims are described below. It will be apparent that the aspects described herein can be embodied in a wide variety of forms, and any particular structure and / or function described herein is merely illustrative. Based on this application, those skilled in the art will understand that one aspect described herein can be implemented independently of any other aspect, and two or more of these aspects can be combined in various ways. For example, any number and aspects set forth herein can be used to implement the device and / or practice the method. Additionally, this device and / or method can be implemented using structures and / or functionalities other than one or more of the aspects set forth herein.
[0031] It should also be noted that the illustrations provided in the following embodiments are only schematic representations of the basic concept of this application. The drawings only show the components related to this application and are not drawn according to the actual number, shape and size of the components in the actual implementation. In the actual implementation, the form, quantity and proportion of each component can be arbitrarily changed, and the layout of the components may also be more complex.
[0032] Additionally, specific details are provided in the following description to facilitate a thorough understanding of the examples. However, those skilled in the art will understand that practice can be carried out without these specific details.
[0033] The technical solutions provided by the various embodiments of this application are described below with reference to the accompanying drawings.
[0034] See Figures 1 to 5 This utility model provides a paraffin embedding and fixation device for organoid tissues. In order to make the practical purpose, technical solution and advantages of this utility model clearer, the specific operation process of this utility model will be described in detail below with reference to the accompanying drawings and the working process of this device.
[0035] This utility model provides a paraffin embedding and fixation device for organoid tissues, including an embedding box 1, a frame 2 around the embedding box 1, a cavity 3 inside the frame 2, a wax pouring groove 4 between the cavities 3, an embedding groove 6 inside the cavity 3, and a depth groove 5 on the embedding groove 6.
[0036] See Figures 1 to 5The cavity 3 is a square, and there are multiple cavities 3. The multiple cavities 3 are the same size and are arranged side by side with equal spacing. The multiple cavities 3 are symmetrically arranged with respect to the embedding groove 6. The embedding groove 6 is located at the center of symmetry of the frame 2. The axis of the embedding groove 6 and the axis of the depth groove 5 are on the same straight line. The depth groove 5 is a multi-level gradient groove and is located at the center of the embedding box 1. The wax pouring groove 4 is located between the embedding groove 6 and the multiple cavities 3. There are multiple wax pouring grooves 4. The horizontal cross section of the embedding groove 6 is a ring. The embedding groove 6 is located at the center of symmetry of the embedding box 1. The multiple wax pouring grooves 4 are located on the embedding box 1 and are distributed at equal intervals.
[0037] The working principle and specific workflow of the paraffin-embedded fixation device for organoid tissues are as follows:
[0038] The designed paraffin embedding and fixation device for organoid tissue is placed horizontally. The organoid tissue to be embedded is taken out and placed into the embedding groove 6 of the fixation device. This ensures that the tissue block can maintain a fixed position during the embedding process, avoiding movement or tilting, and ensuring the integrity of the organoid tissue, thus providing support for subsequent experiments. The structural design of this embedding and fixation device effectively protects the organoid tissue block during the embedding process, avoiding damage or deformation caused by improper operation or external force.
[0039] During the embedding process, the embedding agent is poured into the wax-pouring groove 4 between the cavities 3. The embedding agent flows from the wax-pouring groove 4 to the embedding groove 6 below, ensuring that the organoid tissue block is uniformly and tightly wrapped in the embedding agent. The design of the wax-pouring groove 4 and the embedding groove 6 helps to accurately locate the position of the tissue block, playing a good positioning and guiding role. At the same time, it plays a supporting role during the process of detaching from the embedding box 1. The strip structure formed by the wax-pouring groove 4 can support the entire paraffin block and prevent the paraffin block from breaking during the detachment process.
[0040] Because the embedding groove 6 is spirally ascending, and the embedding groove 6 is provided with a depth groove 5 to indicate the thickness of the embedding section, it can indicate the cutting depth during the slicing process, thereby avoiding the loss of organoid tissue due to excessive depth or the incomplete cutting of organoids due to insufficient depth.
[0041] The same or similar parts between the various embodiments in this specification can be referred to mutually. Each embodiment focuses on describing the differences from other embodiments.
[0042] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.
Claims
1. An organoid tissue paraffin-embedding fixation device, characterized by: The application relates to an embedding box (1) which is provided with a frame (2) around the embedding box (1), a cavity (3) is arranged in the frame (2), a wax pouring groove (4) is arranged between the cavities (3), a embedding groove (6) is arranged on the inner side of the cavity (3), and a depth groove (5) is arranged on the embedding groove (6).
2. The organoid tissue paraffin-embedding fixation device of claim 1, wherein: The cavity (3) is a square body, and a plurality of cavities (3) are arranged.
3. The organoid tissue paraffin-embedding fixture of claim 1, wherein: The plurality of cavities (3) are arranged at equal intervals and side by side, and the plurality of cavities (3) are symmetrically arranged relative to the embedding groove (6).
4. The organoid tissue paraffin-embedding fixture of claim 1, wherein: The embedding groove (6) is located at the center of the frame (2), and the axis of the embedding groove (6) is located on the same straight line as the axis of the depth groove (5).
5. The organoid tissue paraffin-embedding fixture of claim 1, wherein: The depth groove (5) is a multi-step gradient groove, and the depth groove (5) is located at the center of the embedding box (1).
6. The organoid tissue paraffin-embedding fixture of claim 1, wherein: The wax pouring groove (4) is located between the embedding groove (6) and the plurality of cavities (3), and a plurality of wax pouring grooves (4) are arranged.
7. The organoid tissue paraffin-embedding fixture of claim 1, wherein: The horizontal section of the embedding groove (6) is a circular ring, and the embedding groove (6) is located at the center of the embedding box (1).
8. The organoid tissue paraffin-embedding fixture of claim 1, wherein: A plurality of wax pouring grooves (4) are arranged on the embedding box (1), and the plurality of wax pouring grooves (4) are arranged at equal intervals and cross each other.