Fluorescent quantitative polymerase chain reaction detection kit
By inserting the PCR reaction solution, standards, controls, and extraction lysis buffer into the insert protective sponge and surrounding them with a top protective film, the risk of shaking and detachment of the test solution bottles during transportation is eliminated, ensuring the safety and stability of the test solutions in the kit.
Patent Information
- Application Number
- CN202521049520.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-27
- Publication Date
- 2026-01-16
- Estimated Expiration
- 2035-05-27
AI Technical Summary
In existing quantitative PCR detection kits, the test solution bottles are prone to shaking during transportation due to the semi-insertion method, which affects stability and increases the risk of detachment from the sponge retaining layer.
The PCR reaction solution, standards, controls, and extraction lysis buffer are all inserted into the protective sponge of the insert, and then completely surrounded by the top protective film to ensure that the liquid bottles do not shake during transportation and reduce the risk of detachment.
This effectively prevents the liquid bottle from shaking during transportation, improving the safety and stability of the test solution inside the kit.
Smart Images
Figure CN223804098U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the technical field of reagent box especially relates to a fluorescent quantitative polymerase chain reaction detection reagent box. BACKGROUND
[0002] The fluorescent quantitative polymerase chain reaction detection reagent box is a molecular biology detection tool based on PCR technology, which uses fluorescent dyes or fluorescently labeled primers to monitor the changes of DNA amplification products in real time, and can quantitatively analyze target nucleic acids.
[0003] In the existing fluorescent quantitative polymerase chain reaction detection reagent box actual application scene, in order to facilitate the operator to take test liquid when detecting, the test liquid bottle is generally fixed in the sponge holding layer in the reagent box by half-inserting mode, however, the test liquid bottle is prone to shaking during transportation because it is only partially embedded in the sponge holding layer, which not only may affect the stability of the test liquid, but also significantly increases the risk of the test liquid bottle separating from the sponge holding layer compared with the full-inserting mode. SUMMARY
[0004] The utility model embodiment relates to a kind of fluorescent quantitative polymerase chain reaction detection reagent box, which inserts the PCR reaction liquid, standard, control and extraction lysate inside the protection sponge of insertion by full, effectively prevent the phenomenon that above-mentioned liquid bottle shakes when reagent box is transported, the reagent box not only inserts the liquid bottle inside the protection sponge of insertion by full, but also cooperates with top protection film to protect the liquid bottle by full, further reduce the situation that the liquid bottle separates from the protection sponge of insertion, further ensure the safety of test liquid inside the detection reagent box.
[0005] The utility model discloses a fluorescent quantitative polymerase chain reaction detection reagent box, which is characterized by the following: a reagent box body is provided, a fixed base is fixedly installed at the bottom of the inside of the reagent box body, limit clamping seats are fixedly installed on the top of the fixed base, limit clamping pieces are fixedly installed at the top of the inside of the limit clamping seats, a protection sponge of insertion is arranged on the top of the fixed base, a PCR reaction liquid is inserted into the right side of the inside of the protection sponge of insertion, a protection cover plate is slidably installed in the inside of the reagent box body, breakthrough perforations A are formed in the top right side of the protection cover plate, breakthrough perforations B are formed in the front end of the top left side of the protection cover plate, breakthrough perforations C are formed in the rear end of the top left side of the protection cover plate, breakthrough perforations D are formed in the top of the protection cover plate, top protection films are arranged in the inside of the breakthrough perforations A, the breakthrough perforations B, the breakthrough perforations C and the breakthrough perforations D, and easy-to-tear lines are formed in the top protection films.
[0006] Further, the limit clamping seats are in the shape of a kappa, and the limit clamping pieces are in an inclined structure.
[0007] Further, a standard product is inserted at the front end position of the left side inside the insertion protection sponge; and a control product is inserted at the rear end position of the left side inside the insertion protection sponge.
[0008] Further, the two control products are positive control and negative control respectively; an extraction lysis solution is inserted inside the insertion protection sponge; and the extraction lysis solution is located between the standard product and the control product.
[0009] Further, the protection cover plate is located at the top of the insertion protection sponge.
[0010] Further, limit cards are fixedly installed at the bottom of the left and right sides of the protection cover plate; and limit card heads are fixedly installed at the bottom end of the limit cards.
[0011] Further, the limit card head and the limit card jointly form an inverted T-shaped structure.
[0012] The utility model provides a fluorescent quantitative polymerase chain reaction detection kit, has the following beneficial effects:
[0013] 1. In the detection kit, the placement of PCR reaction liquid, standard product, control product and extraction lysis solution is all embedded inside the insertion protection sponge in full insertion mode, and in the transportation process of the kit, the liquid bottle can be effectively prevented from shaking, and the transportation process is ensured to be stable and orderly.
[0014] 2. The detection kit not only embeds the liquid bottle inside the insertion protection sponge in full insertion mode, but also additionally adopts a top protection film, the top protection film can wrap the liquid bottle in all directions in cooperation with the insertion protection sponge, forms more rigorous protection, further reduces the possibility that the liquid bottle is separated from the insertion protection sponge, and better guarantees the safety of the test liquid in the kit. BRIEF DESCRIPTION OF DRAWINGS
[0015] In order to more clearly illustrate the technical scheme of the embodiments of the utility model, the drawings of the embodiments will be briefly introduced below.
[0016] The drawings in the following description only relate to some embodiments of the utility model, and are not limited to the utility model.
[0017] In the drawings:
[0018] Figure 1 A schematic view showing the overall structure of the utility model is shown;
[0019] Figure 2 A structure schematic view showing the split state of the protection cover plate of the utility model is shown;
[0020] Figure 3The utility model discloses a protective cover plate and insert protection sponge structure schematic diagram show;
[0021] Figure 4 The utility model discloses a fixed base and protective cover plate structure schematic diagram show;
[0022] Figure 5 The utility model discloses a Figure 4 The side view perspective structure schematic diagram shows;
[0023] List of reference signs
[0024] 1, kit body;2, fixed base;3, limit card seat;4, limit card piece;5, insert protection sponge;6, PCR reaction liquid;7, standard product;8, control product;9, extract lysis fluid;10, protective cover plate;11, limit card;12, limit card head;13, breakthrough perforation A;14, breakthrough perforation B;15, breakthrough perforation C;16, breakthrough perforation D;17, top protection film. Specific embodiments
[0025] To make the utility model embodiment's purpose, technical scheme and advantage more clear, below will combine the drawings of the utility model embodiment, and the technical scheme of the utility model embodiment is clearly and completely described. Obviously, the described embodiment is a part of the embodiment of the utility model, not all embodiments. Based on the described embodiment of the utility model, all other embodiments obtained by the ordinary skill in the art without the premise of creative labor belong to the range of the utility model protection.
[0026] Please refer to Figures 1 to 5 : example one,
[0027] The utility model provides a kind of fluorescent quantitative polymerase chain reaction detection kit, comprising: kit body 1, the inside bottom end of kit body 1 is fixedly installed with fixed base 2;The top left and right sides of fixed base 2 are fixedly installed with limit card seat 3;The inside top end of limit card seat 3 is fixedly installed with limit card piece 4;The top of fixed base 2 is provided with plug-in protection sponge 5;PCR reaction liquid 6 is inserted in the inside right side of plug-in protection sponge 5;Protection cover plate 10 is slidably installed in the inside of kit body 1;Breakthrough perforation A13 is formed in the top right side of protection cover plate 10;Breakthrough perforation B14 is formed in the top left side of protection cover plate 10;Breakthrough perforation C15 is formed in the top left side of protection cover plate 10;Breakthrough perforation D16 is formed in the top of protection cover plate 10;Top protection film 17 is arranged in the inside of breakthrough perforation A13, breakthrough perforation B14, breakthrough perforation C15 and breakthrough perforation D16;Easily tear line is formed in top protection film 17, kit body 1 is opened, now protection cover plate 10 and top protection film 17 are exposed, then protection cover plate 10 is pressed downward by artificial, and protection cover plate 10 is compressed to plug-in protection sponge 5, and plug-in protection sponge 5 is cancelled to the surrounding of PCR reaction liquid 6, standard 7, control 8 and extraction lysate 9 in compression process, so that PCR reaction liquid 6, standard 7, control 8 and extraction lysate 9 protrude plug-in protection sponge 5 and break through top protection film 17.
[0028] In the embodiment two, on the basis of the embodiment one, the limit card seat 3 is in the structure of the letter N;The limit card piece 4 is in the structure of the inclination;The standard 7 is inserted in the inside left side of the plug-in protection sponge 5 at the front end position;The control 8 is inserted in the inside left side of the plug-in protection sponge 5 at the rear end position;The two controls 8 are positive control and negative control respectively;The extraction lysate 9 is inserted in the inside of the plug-in protection sponge 5;The extraction lysate 9 is between the standard 7 and the control 8, after the protection cover plate 10 is lowered to a certain position, the limit card head 12 will push the limit card piece 4, so that it is deformed and shrinks under pressure, until the limit card head 12 completely enters the inside of the limit card seat 3, at this time, the limit card piece 4 restores and resists above the limit card head 12 under the action of its elasticity, so that the protection cover plate 10 is kept in the state of compressing the plug-in protection sponge 5, and most of the PCR reaction liquid 6, the standard 7, the control 8 and the extraction lysate 9 are exposed, which is convenient for the user to take and use the PCR reaction liquid 6, the standard 7, the control 8 and the extraction lysate 9.
[0029] In the third embodiment, the protective cover plate 10 is located on the top of the insertion protective sponge 5, the bottom of the protective cover plate 10 is fixedly installed with the limiting card 11 on the left and right sides, the bottom end of the limiting card 11 is fixedly installed with the limiting card head 12, the limiting card head 12 and the limiting card 11 jointly form a reverse T-shaped structure, the PCR reaction liquid 6, the standard 7, the control 8 and the extraction lysis liquid 9 are all inserted into the insertion protective sponge 5, the shaking phenomenon of the liquid bottle during the transportation of the kit is effectively prevented, the liquid bottle is not only inserted into the insertion protective sponge 5, but also surrounded and protected by the top protective film 17, the liquid bottle is further prevented from separating from the insertion protective sponge 5, and the safety of the test liquid in the kit is further ensured.
[0030] Working principle: in use, first, the kit body 1 is opened, at this time, the protective cover plate 10 and the top protective film 17 are exposed, then the protective cover plate 10 is pressed downward by hand, the protective cover plate 10 is compressed to the insertion protective sponge 5, in the compression process, the insertion protective sponge 5 cancels the surrounding of the PCR reaction liquid 6, the standard 7, the control 8 and the extraction lysis liquid 9, the PCR reaction liquid 6, the standard 7, the control 8 and the extraction lysis liquid 9 protrude from the insertion protective sponge 5 and break the top protective film 17, and after the protective cover plate 10 is lowered to a certain position, the limiting card head 12 pushes the limiting card 4, the limiting card 4 is deformed and shrinks under pressure, until the limiting card head 12 completely enters the limiting card seat 3, at this time, the limiting card 4 is restored under the elastic action of itself and is resisted above the limiting card head 12, so that the protective cover plate 10 is kept in a state of compressing the insertion protective sponge 5, most of the PCR reaction liquid 6, the standard 7, the control 8 and the extraction lysis liquid 9 are exposed, and the user can take and use the PCR reaction liquid 6, the standard 7, the control 8 and the extraction lysis liquid 9.
[0031] In this paper, the following points need attention:
[0032] 1. The embodiment of the utility model only relates to the structure involved in the embodiment of the utility model, and other structures can refer to the general design.
[0033] 2. In the case of no conflict, the embodiments of the utility model and the features in the embodiments can be combined to obtain new embodiments.
[0034] The above is only a specific implementation manner of the utility model, but the protection scope of the utility model is not limited to this, any person skilled in the art can easily think of changes or replacements within the technical range disclosed by the utility model, which should be covered in the protection scope of the utility model. Therefore, the protection scope of the utility model should be subject to the protection scope of the claims.
Claims
1. A fluorescent quantitative polymerase chain reaction detection kit, comprising: The kit body (1) is internally fixedly installed with a fixed base (2) at the bottom end; the fixed base (2) is fixedly installed with a limiting clamping seat (3) at the top of the left and right sides; characterized in that the limiting clamping seat (3) is fixedly installed with a limiting clamping piece (4) at the top end inside; the fixed base (2) is provided with an inserted protective sponge (5) at the top; the inserted protective sponge (5) is inserted with a PCR reaction liquid (6) at the right side inside; the kit body (1) is internally slidably installed with a protective cover plate (10); the protective cover plate (10) is provided with a breakthrough perforation A (13) at the right side of the top; the front end of the left side of the top of the protective cover plate (10) is provided with a breakthrough perforation B (14); the rear end of the left side of the top of the protective cover plate (10) is provided with a breakthrough perforation C (15); the top of the protective cover plate (10) is provided with a breakthrough perforation D (16); the inside of the breakthrough perforation A (13), the breakthrough perforation B (14), the breakthrough perforation C (15) and the breakthrough perforation D (16) is provided with a top protective film (17); the top protective film (17) is provided with a tear line.
2. The fluorescent quantitative polymerase chain reaction detection kit according to claim 1, characterized in that, The limiting clamping seat (3) is in a V-shaped structure; the limiting clamping piece (4) is in an inclined structure.
3. The fluorescent quantitative polymerase chain reaction detection kit according to claim 2, characterized in that, The inserted protective sponge (5) is inserted with a standard product (7) at the front end position of the left side inside; the inserted protective sponge (5) is inserted with a control product (8) at the rear end position of the left side inside.
4. The fluorescent quantitative polymerase chain reaction detection kit according to claim 3, characterized in that, Two control products (8) are respectively positive control and negative control; the inserted protective sponge (5) is internally inserted with an extraction lysis liquid (9); the extraction lysis liquid (9) is located between the standard product (7) and the control product (8).
5. The fluorescent quantitative polymerase chain reaction detection kit according to claim 4, characterized in that, The protective cover plate (10) is located at the top of the inserted protective sponge (5).
6. The fluorescent quantitative polymerase chain reaction detection kit according to claim 5, characterized in that, The bottom of the protective cover plate (10) is fixedly installed with a limiting clamping card (11) at the left and right sides; the limiting clamping card (11) is fixedly installed with a limiting clamping head (12) at the bottom end.
7. The fluorescent quantitative polymerase chain reaction detection kit according to claim 6, characterized in that, The limiting clamping head (12) and the limiting clamping card (11) jointly form an inverted T-shaped structure.