Novel breaking pipe of DNA breaking instrument

Abstract

The utility model discloses a novel breaking pipe of a DNA breaking instrument, and relates to the field of biological test equipment, the novel breaking pipe comprises a pipe body and a pipe cover installed at the top of the pipe body in a threaded mode, a threaded column is fixedly connected to the center of the inner top wall of the pipe cover, a threaded cylinder is connected to the surface of the threaded column in a threaded mode, and a through hole is formed in the bottom of the threaded cylinder; an adsorption column penetrates through the interior of the through hole, the end, located in the threaded cylinder, of the adsorption column is detachably connected with a metal connector, a spring is arranged between the bottom of the metal connector and the inner bottom wall of the threaded cylinder, and the surface of the adsorption column is sleeved with the spring. The detachable connecting structures are arranged between the structural parts, so that classified recycling of different materials in the later period is facilitated.

Description

TECHNICAL FIELD

[0001] The application relates to the field of biological test equipment, in particular to a novel breaking tube of a DNA breaking instrument. BACKGROUND

[0002] According to the principle of second-generation sequencing, DNA extraction and detection need to be performed on experimental materials before sequencing, and appropriate library construction methods are selected according to different DNA concentrations and fragment sizes, wherein long DNA fragments need to be broken. The current mainstream breaking methods include physical mechanical breaking (ultrasonic breaking) and chemical breaking (enzyme digestion and cutting, also known as enzyme cutting method). The enzyme cutting method has sequence preference, is time-consuming, and has high requirements on DNA quality, and is not suitable for some low-quality DNA samples. The ultrasonic breaking method occupies a large market due to its small sample loss, high processing efficiency, and no cross contamination. The working principle of the ultrasonic breaking method is that the breaking instrument converts an electric signal into a high-frequency acoustic signal through a piezoelectric transducer, generates millions of water molecule 'cavities', utilizes the instantaneous fluid shear force generated by the expansion and rupture of the 'cavities' within a few microseconds to break, homogenize and mix the sample in an isothermal and non-contact manner.

[0003] A novel breaking tube of a DNA breaking instrument disclosed by the Chinese utility model (publication number: CN217479448U) comprises a tube body and a tube cover, a connecting belt is arranged between the tube body and the tube cover, the tube body and the tube cover are screw-connected and installed, a screw thread connection area, a limiting block and a connecting ring are arranged on the outer wall of the tube mouth, the tube body below the connecting ring is divided into an adaptation area and a breaking area, and an adsorption column is arranged in the tube body. The adsorption column is suspended in the middle of the tube body through spring connection, can vibrate during ultrasonic breaking, and achieves the purposes of homogenization and adsorption.

[0004] However, the adsorption column and the spring and other parts are fixedly installed in the interior of the tube body, which is inconvenient to disassemble, and is not conducive to recycling of different materials in the later period. Therefore, the present application provides a novel breaking tube of a DNA breaking instrument. Content of the utility model

[0005] In order to facilitate the recycling of different material parts in the breaking tube, the application provides a novel breaking tube of a DNA breaking instrument.

[0006] The novel breaking tube of the DNA breaking instrument provided by the application adopts the following technical scheme:

[0007] The utility model provides a new breaking tube of DNA breaking instrument, including the pipe body and the pipe cover of screw thread installation in the top of pipe body, the centre of the inner top wall of pipe cover is fixedly connected with the threaded column, the surface of threaded column is connected with the threaded cylinder, the bottom of threaded cylinder is provided with through -hole, the inside of through -hole is penetrated with the adsorption column, the one end of adsorption column in threaded cylinder is detachably connected with the metal connector, the spring is arranged between the bottom of metal connector and the inner bottom wall of threaded cylinder, the spring is sleeved on the surface of adsorption column.

[0008] By adopting the technical scheme, the detachable connection structure is arranged between the structural parts, so that the classification recovery of different materials is facilitated.

[0009] Preferably, the diameter of the adsorption column is smaller than the diameter of the through hole, and the diameter of the metal connector is smaller than the inner diameter of the threaded cylinder.

[0010] By adopting the technical scheme, the adsorption column can not only move up and down, but also move in the horizontal direction, so that the homogenization effect is improved.

[0011] Preferably, a threaded head is fixedly installed on one side of the metal connector, a threaded groove is formed in one end of the adsorption column, and the threaded head is screw-connected in the interior of the threaded groove.

[0012] By adopting the technical scheme, the threaded head and the threaded groove are arranged, so that the disassembly and assembly of the metal connector and the adsorption column are facilitated, thereby facilitating the classification recovery of the metal connector and the adsorption column.

[0013] Preferably, adsorption plates are fixedly installed on the surface of the adsorption column in a symmetrical manner, and leakage holes are formed in the adsorption plates.

[0014] By adopting the technical scheme, when the adsorption column moves up and down due to the vibration generated in the ultrasonic breaking process, the adsorption plates move up and down, and the leakage holes arranged on the adsorption plates are matched, so that the homogenization and adsorption of water molecules "cavities" are achieved.

[0015] Preferably, anti-skid lines are arranged on the surface of the threaded cylinder away from the pipe cover.

[0016] By adopting the technical scheme, the anti-skid lines are arranged, so that the threaded cylinder is conveniently screwed, and the disassembly and assembly of the threaded cylinder and the threaded column are facilitated.

[0017] Preferably, a sealing ring gasket is installed between the pipe cover and the pipe body, and the sealing ring gasket is glued to the inner top wall of the pipe cover.

[0018] By adopting the technical scheme, the sealing ring gasket is arranged, so that the sealing property between the pipe cover and the pipe body is improved.

[0019] Preferably, the inner wall of the sealing ring pad is fixedly connected with a pull ring, and the pull ring and the sealing ring pad are an integral structure.

[0020] By adopting the technical scheme, the sealing ring pad glued to the pipe cover can be separated from the pipe cover conveniently through the pull ring, so that different materials can be classified and recycled.

[0021] In summary, the present application has at least one of the following beneficial technical effects:

[0022] 1. After the sample is added, the pipe body is sealed with the pipe cover and then put into the ultrasonic DNA breaking instrument, the ultrasonic DNA breaking instrument focuses acoustic energy on the sample, and the sample in the pipe body is broken and mixed by ultrasonic waves in an isothermal and non-contact manner. In this process, the adsorption column moves up and down, driving the adsorption plate to move up and down, cooperating with the leakage hole arranged on the adsorption plate, achieving the purpose of homogenization and adsorbing water molecules "cavities". At the same time, the adsorption column can also shake in the horizontal direction, improving the homogenization effect.

[0023] 3. The pipe cover and the pipe body are separated by screwing, and then the threaded cylinder is screwed. When the threaded cylinder and the threaded column are separated, the adsorption column is pushed out from the inside of the threaded cylinder. The metal connecting head and the adsorption column are separated by screwing, the spring is taken out of the adsorption column, and the adsorption column is pulled out of the through hole at the bottom of the threaded cylinder. Finally, the sealing ring pad glued to the pipe cover is separated from the pipe cover by pulling the pull ring, the structure parts of the device are removed, and the classification and recycling of different materials are facilitated. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall structure of a new breaking pipe of a DNA breaking instrument according to an embodiment of the present application;

[0025] Figure 2 It is a schematic diagram of the connection structure of the adsorption column and the adsorption plate according to an embodiment of the present application;

[0026] Figure 3 It is a schematic diagram of the threaded cylinder structure according to an embodiment of the present application;

[0027] Figure 4 It is a schematic diagram of the structure of the sealing ring pad and the pull ring according to an embodiment of the present application;

[0028] Reference signs: 1, pipe body; 2, pipe cover; 3, threaded column; 4, threaded cylinder; 5, through hole; 6, adsorption column; 7, metal connecting head; 8, threaded head; 9, threaded groove; 10, adsorption plate; 11, leakage hole; 12, anti-slip pattern; 13, sealing ring pad; 14, pull ring; 15, spring. DETAILED DESCRIPTION

[0029] The following will be described in combination with the drawingsFigures 1-4 The application is further described in detail.

[0030] The application discloses a novel breaking tube of a DNA breaking instrument.

[0031] Embodiment 1

[0032] With reference to Figures 1-4 The novel breaking tube of the DNA breaking instrument comprises a tube body 1 and a tube cover 2 threadedly installed on the top of the tube body 1, a sealing ring pad 13 is installed between the tube cover 2 and the tube body 1, the sealing ring pad 13 is glued to the inner top wall of the tube cover 2, and the sealing ring pad 13 is arranged to improve the sealing performance between the tube cover 2 and the tube body 1. The inner wall of the sealing ring pad 13 is fixedly connected with a pull ring 14, the pull ring 14 is an integral structure with the sealing ring pad 13, and the pull ring 14 and the sealing ring pad 13 are made of the same material, i.e., one of rubber and silicone. The sealing ring pad 13 glued to the tube cover 2 can be separated from the tube cover 2 through the arrangement of the pull ring 14, so that different materials can be classified and recycled.

[0033] With reference to Figure 1 , Figure 3 and Figure 4 The inner top wall of the tube cover 2 is fixedly connected with a threaded column 3 at the center, the surface of the threaded column 3 is threadedly connected with a threaded cylinder 4, and the threaded column 3, the threaded cylinder 4, the tube cover 2 and the tube body 1 can be made of the same material. The surface of the end of the threaded cylinder 4 away from the tube cover 2 is provided with anti-skid lines 12, the anti-skid lines 12 are arranged to facilitate the screwing of the threaded cylinder 4, so as to facilitate the disassembly and assembly between the threaded cylinder 4 and the threaded column 3. Further, the upper part of the threaded cylinder 4 extends out of the inside of the tube cover 2, so that the inner wall of the tube cover 2 does not hinder the fingers when the threaded cylinder 4 is screwed.

[0034] With reference to Figures 1-4The bottom of the threaded cylinder 4 is provided with a through hole 5, the inside of the through hole 5 penetrates the adsorption column 6, one end of the adsorption column 6 located inside the threaded cylinder 4 is detachably connected with a metal connector 7, one side of the metal connector 7 is fixedly installed with a threaded head 8, one end of the adsorption column 6 is provided with a threaded groove 9, the threaded head 8 is threadedly connected inside the threaded groove 9, through the setting of the threaded head 8 and the threaded groove 9, the disassembly between the metal connector 7 and the adsorption column 6 is facilitated, thereby facilitating the classification recovery between the metal connector 7 and the adsorption column 6. The bottom of the metal connector 7 and the inner bottom wall of the threaded cylinder 4 are provided with a spring 15, the spring 15 is sleeved on the surface of the adsorption column 6. The surface of the adsorption column 6 is symmetrically fixedly installed with an adsorption plate 10, and the adsorption plate 10 is provided with a leakage hole 11. The ultrasonic DNA breaking instrument focuses the sound wave energy on the sample, and breaks and mixes the sample in the tube body 1 in an isothermal and non-contact manner. When the adsorption column 6 shakes up and down in the tube body 1 due to the vibration generated in the ultrasonic breaking process, the adsorption plate 10 moves up and down, and cooperates with the leakage hole 11 arranged on the adsorption plate 10, to achieve the purpose of homogenization and adsorption of water molecules "cavities". The diameter of the adsorption column 6 is smaller than the diameter of the through hole 5, and the diameter of the metal connector 7 is smaller than the inner diameter of the threaded cylinder 4. The setting of this structure makes the adsorption column 6 not only able to shake up and down, but also able to shake in the horizontal direction, thereby improving the homogenization effect.

[0035] The implementation principle of the new breaking tube of the DNA breaking instrument is as follows: in use, after the tube body 1 after adding the sample is sealed with the tube cover 2 and is placed in the ultrasonic DNA breaking instrument, the ultrasonic DNA breaking instrument focuses the sound wave energy on the sample, and breaks and mixes the sample in the tube body 1 in an isothermal and non-contact manner. Moreover, in this process, when the adsorption column 6 shakes up and down in the tube body 1, the adsorption plate 10 moves up and down, and cooperates with the leakage hole 11 arranged on the adsorption plate 10, to achieve the purpose of homogenization and adsorption of water molecules "cavities". At the same time, the adsorption column 6 can also shake in the horizontal direction, thereby improving the homogenization effect.

[0036] When it is necessary to classify and recycle the parts in the device, first, the tube cover 2 is separated from the tube body 1, then the threaded cylinder 4 is screwed, the adsorption column 6 is pushed out from the inside of the threaded cylinder 4 when the threaded cylinder 4 is separated from the threaded column 3, the metal connector 7 is separated from the adsorption column 6 by screwing, the spring 15 is taken out from the adsorption column 6, and the adsorption column 6 is pulled out from the through hole 5 at the bottom of the threaded cylinder 4, finally, the sealing ring pad 13 glued on the tube cover 2 is separated from the tube cover 2 by pulling the pull ring 14, thereby realizing the disassembly of the structure parts of the device, and facilitating the classification and recycling of different materials.

[0037] The above are the preferred embodiments of the present application, which do not limit the protection scope of the present application, therefore: any equivalent changes made on the structure, shape and principle of the present application shall be covered within the protection scope of the present application.

Claims

1. A novel break tube for a DNA disruptor, characterized by: The utility model relates to a pipe cover (2) and threaded installation pipe body (1) top, the centre of the inner top wall of pipe cover (2) is fixedly connected with threaded column (3), the surface of threaded column (3) is threadedly connected with threaded cylinder (4), the bottom of threaded cylinder (4) is provided with through -hole (5), the inside of through -hole (5) is penetrated with adsorption column (6), one end of adsorption column (6) is detachably connected with metal connector (7) in threaded cylinder (4) inside, the inner bottom wall between metal connector (7) bottom and threaded cylinder (4) is provided with spring (15), spring (15) is sleeved on the surface of adsorption column (6).

2. A novel break tube for a DNA disruptor according to claim 1, characterized in that: The diameter of the adsorption column (6) is less than the diameter of the through hole (5), and the diameter of the metal connector (7) is less than the inner diameter of the threaded cylinder (4).

3. A novel break tube for a DNA disruptor according to claim 1, characterized in that: A threaded head (8) is fixedly installed on one side of the metal connector (7), and a threaded groove (9) is formed in one end of the adsorption column (6).

4. A novel break tube for a DNA disruptor according to claim 1, characterized in that: A plurality of adsorption plates (10) are symmetrically fixedly installed on the surface of the adsorption column (6), and a plurality of leakage holes (11) are formed in the adsorption plates (10).

5. A novel break tube for a DNA disruptor according to claim 1, characterized in that: A plurality of anti-skid lines (12) are formed on the surface of the threaded cylinder (4) away from the pipe cover (2).

6. A novel break tube for a DNA disruptor according to claim 1, characterized in that: A sealing ring gasket (13) is installed between the pipe cover (2) and the pipe body (1), and the sealing ring gasket (13) is glued to the inner top wall of the pipe cover (2).

7. A novel break tube for a DNA disruptor according to claim 6, characterized in that: A pull ring (14) is fixedly connected to the inner wall of the sealing ring gasket (13), and the pull ring (14) and the sealing ring gasket (13) are an integral structure.

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