Incubation plate and incubation device

Abstract

The utility model provides an incubation tray and incubation device, the incubation tray includes incubation tray body and conical liquid pouring mouth, incubation tray body is composed of bottom surface, confluence baffle wall on head both sides, left and right side wall and tail wall, conical liquid pouring mouth is rectangular pyramid, and is composed of isosceles triangle bottom surface and right triangle side wall on both sides; the bottom surface of the incubation plate body comprises an isosceles trapezoid-shaped head groove bottom, a first transition part, a rectangular middle groove bottom, a second transition part and a rectangular tail groove bottom, the head groove bottom and the middle groove bottom are tilted forwards and upwards, and the tail groove bottom is tilted backwards and upwards. According to the incubation plate provided by the utility model, liquid is not hung on the wall when being poured, and a protein transport membrane can be wetted by less liquid in the shaking process.

Description

TECHNICAL FIELD

[0001] The utility model relates to medical detection instrument technical field, concretely relates to an incubation tray and incubation device. BACKGROUND

[0002] Western Blot (WB) is a kind of routine protein analysis technology, is commonly used to identify certain protein, and can be qualitative and semi-quantitative analysis to protein.In Western Blot experiment, the incubation experiment step carried out in incubation tray is extremely crucial.In incubation tray, there are three steps of liquid adding, incubation and liquid pouring, and repeat several rounds.Currently, the incubation tray equipped with protein blotter equipment on market is generally the box body with inclined side wall of liquid pouring mouth, and there is wall-hanging residual phenomenon in liquid pouring link, causes unnecessary pollution and corrosion.Specifically, one is that liquid in incubation tray is not poured clean, and will accumulate in corner, and two is that after liquid pouring, last drop of liquid will flow to incubation tray bottom along incubation tray liquid pouring mouth outer wall. SUMMARY

[0003] In view of the deficiency of prior art, the utility model aims at providing an incubation tray without wall-hanging liquid pouring.

[0004] To achieve the above-mentioned purpose, the utility model adopts the following technical scheme:

[0005] The utility model provides an incubation tray, including incubation tray body and conical liquid pouring nozzle, the incubation tray body is composed of bottom, converging baffle of head two sides, left and right two sides wall and tail wall, the conical liquid pouring nozzle is four pyramid, is composed of isosceles triangle bottom and two sides right-angled triangle side wall, the incubation tray body bottom includes isosceles trapezoidal head groove bottom, first transition part, rectangular middle part groove bottom, second transition part and rectangular tail part groove bottom, the head groove bottom and the middle part groove bottom are inclined to front up, the tail part groove bottom is inclined to back up, the head groove bottom trapezoidal lower bottom edge is connected with the middle part groove bottom front edge through the first transition part, the middle part groove bottom rear edge is connected with the tail part groove bottom front edge through the second transition part, the head converging baffle is connected with the head groove bottom trapezoidal waist edge, the left and right two sides wall is connected with the middle part groove bottom side edge and the tail part groove bottom side edge, the tail wall is connected with the tail part groove bottom rear edge, the head converging baffle is connected with the left and right two sides wall through third transition part respectively, the bottom of the conical liquid pouring nozzle is connected with the head groove bottom trapezoidal upper bottom edge through the fourth transition part, the side wall of the conical liquid pouring nozzle is connected with the head converging baffle through the fifth transition part respectively.

[0006] Further, the middle part groove bottom is provided with blocking column near the first transition part.

[0007] Further, the head groove bottom is provided with a semicircle weir groove outside, and the length of the semicircle weir groove is greater than the length of the upper base of the trapezoidal head groove bottom.

[0008] Further, the incubation disc body is provided with a boss outside the left and right side walls.

[0009] Further, the first transition part is a curved surface tangent to the head groove bottom and the middle groove bottom respectively, the second transition part is a curved surface tangent to the middle groove bottom and the tail groove bottom respectively, the third transition part is a curved surface tangent to the head confluence blocking wall and the left and right side walls respectively, the fourth transition part is a curved surface tangent to the head groove bottom and the bottom surface of the conical liquid pouring nozzle respectively, and the fifth transition part is a curved surface tangent to the side wall of the conical liquid pouring nozzle and the head confluence blocking wall respectively.

[0010] Further, the inclination angle of the head groove bottom is 45°-60°, the inclination angle of the middle groove bottom is 0°-10°, and the inclination angle of the tail groove bottom is 15°-30°.

[0011] Further, the length of the upper base of the trapezoidal head groove bottom is 1 / 3-1 / 2 of the length of the lower base of the trapezoidal head groove bottom.

[0012] Further, the opening angle of the conical liquid pouring nozzle is 20°-40°, and the inclination angle of the bottom surface of the conical liquid pouring nozzle is 15°-30°.

[0013] The utility model also provides a kind of incubation device, including above-mentioned incubation disc, shake platform, platform support, shake drive mechanism, the incubation disc is installed on the shake platform, the shake platform both ends are connected with the platform support, and the shake drive mechanism is installed on the platform support.

[0014] Compared with prior art, the utility model has the beneficial effects that:

[0015] The utility model provides incubation disc liquid pouring does not hang wall, and can use less liquid to wet protein transport membrane in shake process. When pouring, water flow converges to conical liquid pouring nozzle, and water drop is difficult to hang on the outer wall of conical liquid pouring nozzle due to the effect of sharp angle. When there is still a small amount of liquid in incubation disc, water drop at tip is pushed out by following water flow. When there is no liquid in incubation disc, last drop of water drop is collected in tip port instead of hanging on the outer wall of conical liquid pouring nozzle. With the incubation disc back to normal state in pouring state, last drop of water drop flows back to disc, and liquid pouring is clean, and the phenomenon of accumulation and wall hanging residual in corner is avoided. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 Structure diagram of incubation disc Figure 1 ;

[0017] Figure 2 Schematic diagram of the incubation tray Figure 2 ;

[0018] Figure 3 Schematic diagram of the incubation tray Figure 3 ;

[0019] Figure 4 This is a schematic diagram of the incubation device;

[0020] Figure 5 This is a diagram showing the working status of the incubation device.

[0021] Reference numerals: 1-Incubation tray body, 11-Bottom surface, 1101-Head trough bottom, 1102-First transition section, 1103-Middle trough bottom, 1104-Second transition section, 1105-Tail trough bottom, 12-Confluence baffle wall, 1201-Third transition section, 13-Side wall, 14-Tail wall, 2-Conical pouring nozzle, 21-Bottom surface, 2101-Fourth transition section, 22-Side wall, 2201-Fifth transition section, 15-Blocking column, 16-Semi-circular cofferdam trough, 17-Protrusion, 3-Shaking platform, 31-Snap fastener, 4-Platform support, 5-Shaking drive mechanism. Detailed Implementation

[0022] To enable those skilled in the art to more clearly understand the technical means and effects adopted by this utility model to achieve its intended purpose, the following detailed description, in conjunction with the accompanying drawings and preferred embodiments, further illustrates the specific implementation methods, structures, features, and effects of this utility model. The examples given are for illustrative purposes only and are not intended to limit the scope of this utility model.

[0023] In the description of this utility model, it should be noted that the terms "front," "rear," "left," "right," "upper," "lower," "inner," "outer," "horizontal," and "vertical," etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on this utility model. The terms "first," "second," and "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance. In addition, unless otherwise explicitly specified and limited, the terms "installed," "connected," and "linked" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or indirect connections through an intermediate medium; and they can refer to the internal communication of two components. For those skilled in the art, the specific meaning of the above terms in this patent can be understood according to the specific circumstances.

[0024] Referring to Figures 1 to 3 The incubation tray provided by the embodiment comprises an incubation tray body 1 and a conical liquid pouring nozzle 2. The incubation tray body 1 is composed of a bottom surface 11, a head portion converging baffle 12 on both sides, left and right side walls 13 and a tail wall 14. The conical liquid pouring nozzle 2 is a quadrangular pyramid composed of an isosceles triangular bottom surface 21 and two straight triangular side walls 22.

[0025] The bottom surface 11 of the incubation tray body 1 comprises an isosceles trapezoidal head portion groove bottom 1101, a first transition portion 1102, a rectangular middle portion groove bottom 1103, a second transition portion 1104 and a rectangular tail portion groove bottom 1105. The head portion groove bottom 1101 and the middle portion groove bottom 1103 are inclined upwardly forward, and the tail portion groove bottom 1105 is inclined upwardly backward. The inclination angle of the head portion groove bottom 1101 is α, the inclination angle of the middle portion groove bottom 1103 is β, and the inclination angle of the tail portion groove bottom 1105 is γ. The lower base of the trapezoidal head portion groove bottom 1101 is connected to the front edge of the middle portion groove bottom 1103 through the first transition portion 1102, and the first transition portion 1102 is a curved surface tangent to the head portion groove bottom 1101 and the middle portion groove bottom 1103 respectively. The rear edge of the middle portion groove bottom 1103 is connected to the front edge of the tail portion groove bottom 1105 through the second transition portion 1104, and the second transition portion 1104 is a curved surface tangent to the middle portion groove bottom 1103 and the tail portion groove bottom 1105 respectively. The trapezoidal waist of the head portion groove bottom 1101 is connected to the head portion converging baffle 12, the side edges of the middle portion groove bottom 1103 and the tail portion groove bottom 1105 are connected to the left and right side walls 13, and the rear edge of the tail portion groove bottom 1105 is connected to the tail wall 14. The head portion converging baffle 12 is connected to the left and right side walls 13 through the third transition portion 1201 respectively, and the third transition portion 1201 is a curved surface tangent to the head portion converging baffle 12 and the left and right side walls 13 respectively.

[0026] The opening angle of the conical liquid pouring nozzle 2 is δ, and the inclination angle of the bottom surface 21 of the conical liquid pouring nozzle 2 is θ. The bottom surface 21 of the conical liquid pouring nozzle 2 is connected to the upper base of the trapezoidal head portion groove bottom 1101 through the fourth transition portion 2101, and the fourth transition portion 2101 is a curved surface tangent to the head portion groove bottom 1101 and the bottom surface 21 of the conical liquid pouring nozzle 2 respectively. The side walls 22 of the conical liquid pouring nozzle 2 are connected to the head portion converging baffle 12 through the fifth transition portion 2201 respectively, and the fifth transition portion 2201 is a curved surface tangent to the side walls 22 of the conical liquid pouring nozzle 2 and the head portion converging baffle 12 respectively.

[0027] In some preferred embodiments, the inclination angle α of the head portion groove bottom 1101 is 45°-60°, the inclination angle β of the middle portion groove bottom 1103 is 0°-10°, and the inclination angle γ of the tail portion groove bottom 1105 is 15°-30°.

[0028] The middle groove bottom 1103 is inclined upward and forward, and the tail groove bottom 1105 is inclined upward and backward, which can reduce the amount of experimental reagents, and can use less liquid to wet the protein transfer membrane during the shaking of the incubation tray.

[0029] In more preferred embodiments, the angle of the inclination α of the head groove bottom 1101 is 60°, the angle of the inclination β of the middle groove bottom 1103 is 10°, and the angle of the inclination γ of the tail groove bottom 1105 is 30°.

[0030] In some preferred embodiments, the length of the upper base of the trapezoidal head groove bottom 1101 is 1 / 3 to 1 / 2 of the length of the lower base, and in more preferred embodiments, the length of the upper base is 1 / 3 of the length of the lower base.

[0031] In some preferred embodiments, the angle of the opening angle δ of the conical pouring nozzle 2 is 20° to 40°, and the angle of the inclination θ of the bottom surface 21 of the conical pouring nozzle 2 is 15° to 30°. The conical pouring nozzle can converge the water flow to a point during pouring, causing it to slide from the tip in strands, and avoiding wall sticking under the action of the sharp angle.

[0032] In more preferred embodiments, the angle of the opening angle δ of the conical pouring nozzle 2 is 20°, and the angle of the inclination θ of the bottom surface 21 of the conical pouring nozzle 2 is 30°.

[0033] In some preferred embodiments, the middle groove bottom 1103 of the incubation tray body 1 is provided with a plurality of blocking columns 15 on the side close to the first transition 1102. The blocking columns 15 can block the protein transfer membrane from falling off during pouring of the liquid.

[0034] In some preferred embodiments, the outer side of the head groove bottom 1101 is also provided with a semicircular weir groove 16, and the length of the semicircular weir groove 16 is greater than the length of the upper base of the trapezoidal head groove bottom 1101. The semicircular weir groove 16 can play a secondary protection role. If liquid slides down the side walls 22 on both sides of the conical pouring nozzle 2 during pouring, it will be collected in the semicircular weir groove 16, avoiding pollution caused by flowing to the bottom of the incubation tray.

[0035] In some preferred embodiments, the outer side of the side walls 13 on the left and right sides of the incubation tray body 1 is provided with a boss 17. The boss 17 is used for installation and fixation of the incubation tray.

[0036] Referring to Figure 4 The incubation device provided in the embodiments includes the incubation tray, the shaking platform 3, the platform support 4, and the shaking drive mechanism 5. The incubation tray is installed on the shaking platform 3, the shaking platform 3 is provided with a rotating shaft at both ends and is connected with the platform support 4, and the shaking drive mechanism 5 is installed on the platform support 4 to control the rotation of the shaking platform 3 around the rotating shaft.

[0037] In some preferred embodiments, the shaking platform 3 is provided with buckles 31 matching the protrusions 17 on the incubation disc body 1, and the protrusions 17 are detachably connected with the buckles 31.

[0038] Referring to Figure 5 , Figure 5 (a) is a pouring state, Figure 5 (b) is a reverse shaking state, Figure 5 (c) is a forward shaking state. When the incubation disc is pouring, the liquid flows through the head groove bottom 1101, and is collected to the middle under the action of the confluence blocking wall 12, and is gently accumulated to the tapered pouring nozzle 2. With the increasing of the inclination angle, the water flow slides from the tip of the tapered pouring nozzle 2 in strands. Due to the effect of the sharp angle, water droplets are difficult to hang on the outer wall of the tapered pouring nozzle 2. When there is still a small amount of liquid in the incubation disc, the water droplets at the tip will be pushed out by the following water flow. When there is no liquid in the incubation disc, the last drop of water droplets will be collected in the tip port instead of hanging on the outer wall of the tapered pouring nozzle 2, and the last drop of water droplets will flow back to the disc with the upright of the pouring state incubation disc.

[0039] The above is only a preferred embodiment of the present application, and does not limit the present application in any form. Any person skilled in the art can make some changes or modifications to the above disclosed technical content without departing from the scope of the technical scheme of the present application, and any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present application still belong to the scope of the technical scheme of the present application.

Claims

1. An incubation tray, characterized in that, The incubation tray body comprises a bottom surface, head-side converging blocking walls, left and right side walls and a tail wall, and the tapering liquid pouring nozzle is a quadrangular pyramid comprising an isosceles triangular bottom surface and two straight-angled triangular side walls; the bottom surface of the incubation tray body comprises an isosceles trapezoidal head slot bottom, a first transition part, a rectangular middle slot bottom, a second transition part and a rectangular tail slot bottom, the head slot bottom and the middle slot bottom are inclined upward forward, the tail slot bottom is inclined upward backward, the lower base edge of the head slot bottom is connected to the front edge of the middle slot bottom through the first transition part, the rear edge of the middle slot bottom is connected to the front edge of the tail slot bottom through the second transition part, the head-side converging blocking walls are connected to the trapezoidal waist edges of the head slot bottom, the left and right side walls are connected to the side edges of the middle slot bottom and the tail slot bottom, the tail wall is connected to the rear edge of the tail slot bottom, and the head-side converging blocking walls are connected to the left and right side walls through third transition parts respectively.

2. The incubation tray of claim 1, wherein, The middle slot bottom is provided with a blocking column near the first transition part.

3. The incubation tray of claim 1, wherein, The head slot bottom is provided with a semicircular weir slot on the outside, and the length of the semicircular weir slot is greater than the length of the upper base edge of the head slot bottom.

4. The incubation tray of claim 1, wherein, The left and right side walls of the incubation tray body are provided with bosses on the outside.

5. The incubation tray of claim 1, wherein, The first transition part is a curved surface tangent to the head slot bottom and the middle slot bottom respectively, the second transition part is a curved surface tangent to the middle slot bottom and the tail slot bottom respectively, the third transition part is a curved surface tangent to the head-side converging blocking walls and the left and right side walls respectively, the fourth transition part is a curved surface tangent to the head slot bottom and the bottom surface of the tapering liquid pouring nozzle respectively, and the fifth transition part is a curved surface tangent to the side walls of the tapering liquid pouring nozzle and the head-side converging blocking walls respectively.

6. The incubation tray of claim 1, wherein, The inclination angle of the head slot bottom is 45°-60°, the inclination angle of the middle slot bottom is 0°-10°, and the inclination angle of the tail slot bottom is 15°-30°.

7. The incubation tray of claim 1, wherein, The length of the upper base edge of the head slot bottom is 1 / 3-1 / 2 of the length of the lower base edge.

8. The incubation tray of claim 1, wherein, The opening angle of the tapering liquid pouring nozzle is 20°-40°, and the inclination angle of the bottom surface of the tapering liquid pouring nozzle is 15°-30°.

9. An incubation device, characterized by The incubation tray, the shaking platform, the platform support and the shaking drive mechanism are provided, the incubation tray is installed on the shaking platform, the two ends of the shaking platform are connected to the platform support, and the shaking drive mechanism is installed on the platform support.

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