Multi-T-line semi-quantitative detection test paper based on colloidal gold immunochromatography

Abstract

The utility model provides multi-T-line semi-quantitative detection test paper based on colloidal gold immunochromatography, which is characterized in that a sample pad, a colloidal gold combination pad, a chromatography membrane and absorbent paper are sequentially arranged on the upper side of a substrate from front to back, the sample pad, the colloidal gold combination pad, the chromatography membrane and the absorbent paper are sequentially connected, a sample is dropped on the sample pad, and the sample pad is connected with the colloidal gold combination pad. The colloidal gold conjugate pad slowly enters the chromatography membrane, the front middle part of the chromatography membrane is a T-line detection area, a plurality of transverse detection lines are arranged in the T-line detection area and correspond to different concentration threshold values respectively, and a transverse quality control line is further arranged at the rear part of the chromatography membrane. According to the utility model, a multi-T-line design is adopted, so that semi-quantitative detection can be realized, and a relatively accurate basis is provided for clinical diagnosis and disease monitoring; secondly, the test paper is simple in structure, convenient to operate, capable of being used without professional training and suitable for on-site rapid detection.

Description

TECHNICAL FIELD

[0001] The utility model relates to lateral chromatography technical field, specifically, relate to a kind of based on colloidal gold immunochromatography's multiple T line semi-quantitative detection test paper. BACKGROUND

[0002] In the field of biomedical detection and diagnosis, rapid, accurate and convenient detection method has important significance for early screening, diagnosis and monitoring of disease. As a kind of instant detection (POCT) method, colloidal gold immunochromatography technology is widely used because of its simple operation, fast detection speed and no need for complex instruments. However, the traditional colloidal gold immunochromatography test paper can only provide qualitative detection results, that is, to judge whether there is target substance in the sample, which is difficult to meet the demand for more accurate measurement of target substance concentration. In addition, in some detection scenarios, multiple concentration ranges need to be detected at the same time to provide more comprehensive information, but the existing test paper often cannot achieve it. At the same time, the performance of each component of the test paper and the mutual cooperation relationship will directly affect the accuracy and reliability of the detection. Therefore, in order to improve the detection accuracy and information amount of colloidal gold immunochromatography technology, it has important application value to develop a new type of multiple T line semi-quantitative test paper. SUMMARY

[0003] The utility model is just based on above-mentioned technical problem, proposes a kind of based on colloidal gold immunochromatography's multiple T line semi-quantitative detection test paper.

[0004] A kind of based on colloidal gold immunochromatography's multiple T line semi-quantitative detection test paper, including: base, sample pad, colloidal gold combination pad, chromatography membrane, water absorption paper are sequentially arranged on the upper side of the base from front to back, the sample pad, the colloidal gold combination pad, the chromatography membrane, the water absorption paper are sequentially connected, sample is dropped on the sample pad, and the sample is uniformly dispersed, to prepare for the subsequent detection process, then slowly enter the chromatography membrane on the colloidal gold combination pad, colloidal gold combination pad can be combined with target substance in sample, form complex, the front middle part of the chromatography membrane is T line detection area, multiple T line detection area is equipped with the detection line of horizontal direction, multiple the detection line corresponds to different concentration threshold value respectively, by observing the color development of different detection lines, the semi-quantitative detection of target substance concentration can be realized, and more abundant detection information is provided, chromatography membrane rear part is also provided with the horizontal quality control line, for verifying the effectiveness of detection process.

[0005] Preferably, the base is a PVC plate, which provides stable support for each component.

[0006] Preferably, the sample pad is made of glass fiber, which has good adsorption and dispersion performance.

[0007] Preferably, the sample pad, the colloidal gold binding pad, the chromatography membrane and the absorbent paper are staggered from front to back, which facilitates uniform penetration and flow of the sample and improves the accuracy of detection.

[0008] The multi-T-line semi-quantitative test paper based on colloidal gold immunochromatography has the following beneficial effects: firstly, the design of multi-T-line can realize semi-quantitative detection, and provide relatively accurate basis for clinical diagnosis and disease monitoring; secondly, the test paper has simple structure and is convenient to operate, and can be used without professional training, and is suitable for on-site rapid detection; finally, the novel test paper has wide application prospect, and can be used for detection of various biomarkers. BRIEF DESCRIPTION OF DRAWINGS

[0009] Figure 1 The structure schematic diagram of the utility model is shown. DETAILED DESCRIPTION

[0010] In order to make the above, features and advantages of the utility model clearer, the utility model will be described further in detail below in combination with the drawings and specific embodiments. It should be noted that the embodiments and features in the embodiments of the present application can be combined with each other without conflict.

[0011] As Figure 1 shown in a kind of multi-T-line semi-quantitative test paper based on colloidal gold immunochromatography, the test paper includes substrate 1, sample pad 2, colloidal gold binding pad 3, chromatography membrane 4, absorbent paper 5 are sequentially arranged on the upside of substrate 1 from front to back.

[0012] Substrate 1 is made of hard PVC material, provides rigid support for each functional layer of test paper, ensures that test paper still keeps flat under wet state, avoids chromatography path deviation due to deformation, and guarantees detection stability.

[0013] Sample pad 2 is located at the most front end of substrate 1, made of high adsorption glass fiber, its function is to receive liquid sample, make sample quickly and uniformly dispersed by capillary action of fiber aperture, filter out large particle impurities, provide homogenized sample flow for subsequent reaction, the high hydrophilicity design of this material significantly shortens sample penetration time, and improves detection efficiency.

[0014] Colloidal gold binding pad 3 is immediately adjacent to sample pad 2, preloaded with colloidal gold labeled antibody or aptamer inside, when sample flows through this layer, target substance is specifically combined with colloidal gold label, forms colloidal gold label, and the complex continues to migrate with chromatography liquid, provides signal source for color reaction of subsequent detection line 6.

[0015] The middle part of the chromatography membrane 4 is a T line detection area, and a plurality of detection lines 6 (T lines) arranged in the transverse direction are arranged in the T line detection area, each detection line 6 contains an antibody capable of binding to a target substance, and the antibody concentration corresponding to each detection line 6 is optimized by gradient, and corresponds to a preset concentration threshold (such as 0.05-0.5 ng / mL, 0.5-5 ng / mL, 5 ng-10 ng / mL) of the target substance; when the concentration of the target substance in the sample reaches a certain threshold, the corresponding detection line 6 develops color due to the accumulation of antigen-antibody complexes, and by observing the number and position of the colored detection line 6, the concentration interval of the target substance can be directly judged, and semi-quantitative analysis is realized.

[0016] The chromatography membrane 4 is provided with a quality control line 7 in the rear part, and the secondary antibody coated with a colloidal gold label will develop color by combining with the quality control line 7 whether the target substance exists or not, which is used to verify whether the test paper chromatography process is completed and the reagent activity is normal.

[0017] The water absorption paper 5 is located at the end of the test paper, and drives the sample to continuously chromatograph by strong water absorption, avoids liquid backflow or residue, and ensures that the reaction is fully carried out.

[0018] As a further improvement, the sample pad 2, the colloidal gold combination pad 3, the chromatography membrane 4 and the water absorption paper 5 are connected in a partially overlapping manner (the overlapping width is 1-2 mm) on the base 1, and the design improves the detection performance through the following mechanism:

[0019] Uniform penetration control: the staggered structure forms a progressive capillary channel, avoids uneven flow rate of the chromatography liquid at the interface due to sudden change of resistance, and reduces the fluctuation of T line color development intensity;

[0020] Reduce edge effect: the overlapping area can constrain the lateral diffusion of the chromatography liquid, ensure the migration of the complex along the preset path, and improve the color development consistency of different batches of test papers;

[0021] Prolong the reaction time: the partial overlap of the colloidal gold combination pad 3 and the chromatography membrane 4 can delay the speed of the complex entering the detection area, increase the antigen-antibody binding time, and improve the detection sensitivity of low-concentration samples.

[0022] The above only describes preferred embodiments of the present application and is not used to limit the present application. For those skilled in the art, the present application can have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A multi-T line semi-quantitative test paper based on colloidal gold immunochromatography, characterized in that, The application relates to a lateral flow test strip. The lateral flow test strip comprises a base, wherein the upper side of the base is sequentially provided with a sample pad, a colloidal gold combination pad, a chromatographic membrane and water absorption paper from front to back; the sample pad, the colloidal gold combination pad, the chromatographic membrane and the water absorption paper are sequentially connected; sample is dropped on the sample pad and slowly enters the chromatographic membrane through the colloidal gold combination pad; the front middle part of the chromatographic membrane is a T-line detection area; a plurality of transverse detection lines are arranged in the T-line detection area; the plurality of detection lines correspond to different concentration thresholds respectively; and the rear part of the chromatographic membrane is further provided with a transverse quality control line.

2. The colloidal gold immunochromatographic multi-T line semi-quantitative test paper according to claim 1, characterized in that, The base is a PVC adhesive plate.

3. The colloidal gold immunochromatographic multi-T line semi-quantitative test paper according to claim 1, characterized in that, The sample pad is made of glass fiber.

4. The colloidal gold immunochromatographic multi-T line semi-quantitative test paper according to claim 1, characterized in that, The sample pad, the colloidal gold combination pad, the chromatographic membrane and the water absorption paper are staggeredly connected from front to back.

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