Sample treatment instrument

Abstract

The utility model discloses a sample processing instrument, which comprises a sample processing module, a bidirectional driving module, an indirect driving module and / or a non-contact driving module, the sample processing module comprises a first port, a second port, a third port, a sample processing cavity, a flow channel baffle unit and at least one layered processing unit, a direct driving device is not arranged in the sample processing front cavity, and the bidirectional driving module, the indirect driving module and the non-contact driving module are indirect driving devices. On the basis of the mode, particles in the sample cannot be damaged, and the released particles cannot be wound and gathered, so that faults cannot be caused, and the equipment reliability of the sample treatment instrument is greatly improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological sample separation, in particular to a sample processing instrument. BACKGROUND

[0002] In the traditional technology, the method for separating various particles in biological sample liquid or gas, because the particles contain various proteins, when improperly handled, the various particles can be damaged first, and secondly, the particles (such as proteins) can be more likely to be entangled or aggregated, resulting in the failure of subsequent sample processing steps. SUMMARY

[0003] When the sample processing instrument separates the biological sample liquid or gas, how to reduce the possibility of particle damage and reduce the possible entanglement or aggregation of particles (such as proteins), thereby improving the reliability of the sample detection device.

[0004] In order to solve the above technical problems, the technical scheme adopted by the present application is:

[0005] A sample processing instrument, comprising a sample processing module, a bidirectional driving module, further comprising an indirect driving module and / or a non-contact driving module;

[0006] The sample processing module comprises a first port, a second port, a third port, a sample processing cavity, a flow channel baffle unit and at least one layered processing unit,

[0007] The layered processing unit is a multi-layer structure, which divides the sample processing cavity into at least one sample processing front cavity and at least one sample processing rear cavity, and one layer of the multi-layer structure of the layered processing unit in contact with the sample processing front cavity is a contact layer,

[0008] The flow channel baffle unit divides the sample processing front cavity into at least one sample flow channel unit,

[0009] The first port and the second port are in communication with the at least one sample flow channel unit,

[0010] The third port is in communication with the sample processing rear cavity;

[0011] The bidirectional driving module drives the sample to flow into the sample processing front cavity through the first port before the sample processing instrument obtains the sample,

[0012] or, flows out of the sample processing front cavity through the second port,

[0013] or, flows into the sample processing front cavity through the second port,

[0014] or, flows out of the sample processing front cavity through the first port;

[0015] Part of the sample flows into the sample processing post-chamber after passing through the layered processing unit, becoming sample post-sample, and the sample post-sample flows out of the third port;

[0016] The sample processing pre-chamber is free of direct driving devices, and the bidirectional driving module, the indirect driving module, and the non-contact driving module are all indirect driving devices.

[0017] The contact layer is in a plane, the indirect driving module is in fixed contact with the contact layer of the layered processing unit,

[0018] The indirect driving module is a mechanical vibration driving module.

[0019] The mechanical vibration frequency range of the mechanical vibration driving module is 20 Hz to 30 kHz.

[0020] The non-contact driving module is a magnetic field driving module.

[0021] The angle between the magnetic field direction of the magnetic field driving module and the plane in which the contact layer of the layered processing unit is located is greater than or equal to 60 degrees.

[0022] The magnetic field driving module is a Helmholtz coil, and the sample processing module is fixedly arranged between a first coil and a second coil of the Helmholtz coil.

[0023] Or, the magnetic field driving module is at least one permanent magnet, and the sample processing module is fixedly arranged between a south pole and a north pole of the at least one permanent magnet.

[0024] The magnetic field driving module is provided with a first linear Hall sensor and a first linear Hall sensor.

[0025] The first coil and the second coil of the Helmholtz coil have the same size.

[0026] The distance between the centers of the first coil and the second coil is equal to the radius of the first coil.

[0027] The first coil and the second coil are wound in the same direction.

[0028] The current directions of the first coil and the second coil are the same.

[0029] The distance from the first linear Hall sensor to the center of the first coil is equal to the distance from the first linear Hall sensor to the center of the sample processing module.

[0030] The sample processing module is detachable.

[0031] The sample processing instrument includes at least two sample processing modules, and the parameters of the at least two sample processing modules are different.

[0032] Or, the sample processing instrument includes at least one sample processing module or at least one sample separation module.

[0033] Wherein, the bidirectional driving module is a peristaltic pump;

[0034] And / or, the sample processing post-cavity or the third port is connected with a flow monitoring module;

[0035] And / or, the sample processing pre-cavity or the first port or the second port is connected with a parameter measurement module.

[0036] Wherein, the plane where the contact layer of the layered processing unit is located is perpendicular to the ground or the plane where the contact layer of the layered processing unit is located is inclined upward;

[0037] And / or, the pore size of the contact layer of the layered processing unit is smaller than the pore size of other layers of the layered processing unit;

[0038] And / or, the thickness of the contact layer of the layered processing unit is smaller than the thickness of other layers of the layered processing unit.

[0039] Wherein, the layered processing unit includes a first layered processing unit and a second layered processing unit,

[0040] The sample processing module further includes a first sealing elastic unit, a second sealing elastic unit, a first non-elastic cover unit, a second non-elastic cover unit and a fixing structure,

[0041] The fixing structure sequentially fixes the first non-elastic cover unit, the first sealing elastic unit, the first layered processing unit, the flow channel baffle unit, the second layered processing unit, the second sealing elastic unit and the second non-elastic cover unit together;

[0042] And / or, the first sealing elastic unit is provided with at least one first positioning hole, the first non-elastic cover unit or the flow channel baffle unit is provided with at least one first positioning column matched with the at least one first positioning hole, and the at least one first positioning hole and the at least one first positioning column one-to-one correspond;

[0043] And / or, the second sealing elastic unit is provided with at least one second positioning hole, the second non-elastic cover unit or the flow channel baffle unit is provided with at least one second positioning column matched with the at least one second positioning hole, and the at least one second positioning hole and the at least one second positioning column one-to-one correspond.

[0044] Wherein, the sample processing post-cavity includes a first sample processing post-cavity and a second sample processing post-cavity;

[0045] And / or, the first sealing elastic unit is provided with a first hollow groove, the first non-elastic cover unit is provided with a first recess, and the cavity formed by the first recess, the first hollow groove and the first layered processing unit is a first sample post-processing cavity.

[0046] And / or, the second sealing elastic unit is provided with a second hollow groove, the second non-elastic cover unit is provided with a second recess, and the cavity formed by the second recess, the second hollow groove and the second layered processing unit is a second sample post-processing cavity.

[0047] And / or, the first port, the second port and the third port are all arranged on the flow channel baffle unit; and / or, the third port of the flow channel baffle unit is further connected with a horizontal through hole;

[0048] And / or, the first recess on the first non-elastic cover unit is connected with the third port through the horizontal through hole;

[0049] And / or, the second recess on the second non-elastic cover unit is connected with the third port through the horizontal through hole;

[0050] And / or, the third port is arranged downwardly;

[0051] And / or, the horizontal cross-sectional area of the third port near the port gradually decreases from high to low.

[0052] Wherein, when the sample pre-flow passes through the sample pre-processing cavity, the area of the contact layer that can be contacted is the contact area, and the ratio of the contact area to the volume of the sample pre-processing cavity is greater than 10 6 / m.

[0053] Since there is no direct driving device in the sample pre-processing cavity in the sample processing instrument, there is no sharp object directly contacting the sample, which will not damage the particles in the sample, and the bidirectional driving module, the indirect driving module and the non-contact driving module are all indirect driving devices, even if the particles are damaged, the released protein will not cause winding and aggregation phenomenon because the inner wall of the pipeline through which the sample flows is smooth and has no corner dead angle, thereby avoiding causing failure and greatly improving the equipment reliability of the sample processing instrument. BRIEF DESCRIPTION OF DRAWINGS

[0054] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed in the embodiment description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0055] Figure 1 is a structural schematic diagram of one embodiment of the sample detection device of the present application;

[0056] Figure 2 is a structural schematic diagram of one embodiment of the sample detection device of the present application;

[0057] Figure 3 is a structural schematic diagram of one embodiment of the sample detection device of the present application;

[0058] Figure 4 is a structural schematic diagram of one embodiment of the sample detection device of the present application;

[0059] Figure 5 is a structural schematic diagram of one embodiment of the sample detection device of the present application;

[0060] Figure 6 is a structural schematic diagram of one embodiment of the sample detection device of the present application.

[0061] Reference signs: indirect drive module 1, non-contact drive module 2, sample processing module 3, first non-elastic cover unit 31, first sealing elastic unit 32, first layered processing unit 33, flow channel baffle unit 34, second layered processing unit 35, second sealing elastic unit 36, second non-elastic cover unit 37, fixing structure 38, first port 341, second port 342, third port 343, sample processing front cavity 344, positioning column 345, positioning hole 321, sample processing rear cavity 311. DETAILED DESCRIPTION

[0062] The present application will be further described below in conjunction with the drawings and embodiments. It is particularly pointed out that the following embodiments are only for illustrating the present application, but not for limiting the scope of the present application. Similarly, the following embodiments are only part of the embodiments of the present application, but not all the embodiments of the present application. All other embodiments obtained by those skilled in the art without making creative efforts are within the scope of protection of the present application.

[0063] In this text, the phrase "embodiment" means that the specific features, structures or characteristics described in conjunction with the embodiment can be included in at least one embodiment of the present application. The appearance of this phrase in various places in the specification does not necessarily mean the same embodiment, nor is it an independent or alternative embodiment that is not mutually exclusive with other embodiments. Those skilled in the art explicitly and implicitly understand that the embodiments described herein can be combined with other embodiments.

[0064] In the description of the present application, it should be noted that unless otherwise explicitly specified and limited, the terms "mounting", "setting", "connecting", "connecting" should be understood broadly, for example, it can be fixedly connected, it can be detachably connected, or integrally connected; it can be mechanically connected, or electrically connected; it can be directly connected, or connected through an intermediate medium. For those skilled in the art, the above-mentioned specific meanings in the present application can be connected according to the specific circumstances.

[0065] In the conventional technology, the method for separating various particles in the biological sample liquid or gas, because the particles contain various proteins, when improperly handled, the various particles can be damaged first, and secondly, the particles (such as proteins) can be more likely to be entangled or aggregated, resulting in the failure of subsequent sample processing steps.

[0066] Therefore, the present application provides a sample processing instrument, as shown in Figure 1 , Figure 1 is one of the structural schematic diagrams of an embodiment of the sample detection device of the present application, as shown in Figure 1 , comprising a sample processing module 3, a bidirectional driving module, further comprising an indirect driving module 1 and / or a non-contact driving module 2.

[0067] Referring to Figure 2 , Figure 2 is one of the structural schematic diagrams of an embodiment of the sample detection device of the present application, as shown in Figure 2 , the sample processing module 3 comprises a first port 341, a second port 342, a third port 343, a sample processing cavity, a flow channel baffle unit 34 and at least one layered processing unit, the layered processing unit is a multi-layer structure, which divides the sample processing cavity into at least one sample processing front cavity 344 and at least one sample processing rear cavity 311, one layer of the multi-layer structure of the layered processing unit in contact with the sample processing front cavity 344 is a contact layer, the flow channel baffle unit 34 divides the sample processing front cavity 344 into at least one sample flow channel unit, the first port 341 and the second port 342 are both in communication with the at least one sample flow channel unit, and the third port 343 is in communication with the sample processing rear cavity 311; before the sample processing instrument obtains the sample, the bidirectional driving module drives the sample to flow into the sample processing front cavity 344 through the first port 341, or, flows out of the sample processing front cavity 344 through the second port 342, or, flows into the sample processing front cavity 344 through the second port 342, or, flows out of the sample processing front cavity 344 through the first port 341; part of the sample flows into the sample processing rear cavity 311 after passing through the layered processing unit, becoming a sample, and the sample flows out of the third port 343; the sample processing front cavity 344 has no direct driving device, and the bidirectional driving module, the indirect driving module 1 and the non-contact driving module 2 are all indirect driving devices.

[0068] Traditional sample processing instruments often contain direct drive devices such as stirring rods or stirring rods of various shapes, which directly contact the sample fluid and directly drive the sample fluid. The sharp shape generates a large shear force in the sample fluid, which easily destroys the initial morphology of the particles in the sample fluid, causes protein release, and further causes particles (such as proteins) to entangle, aggregate, and even cause the stirring rod or stirring rod to be blocked by the aggregate. Turn, thereby causing equipment failure.

[0069] The sample processing instrument does not have a direct drive device in the sample processing pre-chamber 344, and there is no sharp object directly contacting the sample, which will not destroy the particles in the sample. The bidirectional drive module, indirect drive module 1, and non-contact drive module 2 are all indirect drive devices. Even if the particles are destroyed and the proteins are released, the released particles (such as proteins) will not entangle and aggregate in the smooth and corner-free inner wall of the pipeline through which the sample flows, thereby preventing failure and greatly improving the reliability of the sample processing instrument.

[0070] Further, the bidirectional drive module here can be a peristaltic pump. Peristaltic pumps are pumps that transport fluid by squeezing a flexible tube. It has the following advantages: A, no pollution: since the fluid only contacts the inner wall of the tube, it can avoid pollution inside the pump body, and is suitable for transporting corrosive, toxic or high-purity fluids. B, accurate measurement: peristaltic pumps can accurately control the flow of fluid by adjusting the speed of the motor, suitable for applications that require accurate measurement. C, no pulse delivery: peristaltic pumps do not produce pulses when delivering fluid, and the flow is stable, suitable for situations that require continuous and stable flow. D, reversibility: peristaltic pumps can achieve forward and reverse flow of fluid, making it easy to control the flow direction. E, low shear force: peristaltic pumps have low shear force on the fluid, suitable for transporting shear-sensitive fluids such as living cells or protein solutions.

[0071] Further, the indirect drive module 1 here can be a mechanical vibration drive module that converts the acoustic energy of a power mechanical vibration frequency source into mechanical vibration and radiates mechanical vibration waves to the sample in the sample processing pre-chamber 344 through fixed contact. Due to the radiation of mechanical vibration waves, the microbubbles in the sample in the sample processing pre-chamber 344 can be vibrated under the action of mechanical vibration waves. The adsorption of the adsorbate and the contact layer of the layered processing unit is destroyed, causing the adsorbate to be peeled off by fatigue failure in the contact layer. The fixed contact here includes direct contact and indirect contact.

[0072] Further, the non-contact driving module 2 herein can be a magnetic field separation module. The magnetic separation technology is to deflect the particles with different positive and negative charges in the sample by the action of an external magnetic field, further cause the fatigue failure of the adsorbent in the contact layer and be stripped, so as to achieve the purpose of strengthening the separation of different particles in the sample.

[0073] Further, there is no direct driving device in the pipeline directly contacting the sample fluid in the sample processing instrument, there is no sharp object directly contacting the sample, the particles in the sample will not be damaged, the fluid motion is driven only by the gentle extrusion of the pipeline of the bidirectional driving module, and the adsorbent in the contact layer is stripped by the fatigue failure caused by the indirect driving module 1 and the non-contact driving module 2, so as to achieve the purpose of strengthening the separation of different particles in the sample, and because the inner wall of the pipeline through which the sample flows is smooth without corner dead angle, even if the protein is released, the phenomenon of winding and gathering of particulate matter (such as protein) will not occur, which will not cause failure, and the equipment reliability of the sample processing instrument is greatly improved.

[0074] Further, the layered treatment unit herein can be a separation membrane. The separation membrane is a membrane type material with selective permeation ability. Generally, it can be divided into microfiltration membrane, ultrafiltration membrane, nanofiltration membrane, reverse osmosis membrane, pervaporation membrane, ion exchange membrane, etc. according to separation mechanism and application range. The separation membrane refers to the interface that can limit and transfer fluid substances in a specific form to separate two phases or two parts. The form of the membrane can be solid or liquid. The fluid substance separated by the membrane can be liquid or gas. The separation membrane is a special thin layer material with selective permeation function, which can make one or several substances in the fluid permeate, while other substances do not permeate, thereby playing the role of concentration and separation and purification.

[0075] Further, the contact layer is in a plane, and the indirect driving module 1 is in fixed contact with the contact layer of the layered treatment unit. Since the layered treatment unit herein can be a separation membrane, it is easy to install in a plane, and the fixed contact between the indirect driving module 1 and the contact layer of the layered treatment unit can ensure that the mechanical vibration wave is smoothly transmitted to the contact layer of the layered treatment unit, so as to cause the fatigue failure of the adsorbent in the contact layer and be stripped.

[0076] Further, the indirect driving module 1 herein can be a mechanical vibration driving module, which converts the acoustic energy of a power mechanical vibration frequency source into mechanical vibration, and radiates the mechanical vibration wave to the sample in the sample processing front cavity 344 through fixed contact.

[0077] Further, the mechanical vibration frequency range of the mechanical vibration driving module herein is 5Hz-30KHz; if the frequency is too high, the amplitude is small, the intensity of vibration is insufficient, and the adsorbent cannot be peeled off by fatigue failure in the contact layer; if the frequency is too low, the intensity of vibration is too large, and the multi-layer structure of the layered processing unit is easily damaged. If it is ordinary mechanical vibration, a low frequency of 5-500Hz is also acceptable, and if it is ultrasonic, the mechanical vibration frequency range is 20K-30KHz.

[0078] Further, the non-contact driving module 2 is a magnetic field driving module; the magnetic separation technology is to deflect the particles with different positive and negative charges in the sample by the action of an external magnetic field, further cause the adsorbent to be peeled off by fatigue failure in the contact layer, and thus achieve the purpose of strengthening the separation of different particles in the sample.

[0079] Further, the magnetic field driving module has at least the following two implementation modes:

[0080] Referring to Figure 3 , Figure 3 is one of the structural schematic diagrams of an embodiment of the sample detection device of the present application, as Figure 3 shown, one is that the magnetic field driving module is a Helmholtz coil, and the sample processing module 3 is fixedly arranged between the first coil and the second coil of the Helmholtz coil, which is simple and easy to implement, low in cost, and can realize the adjustment of the magnetic field strength and direction, and is suitable for different scene requirements.

[0081] Two is that the magnetic field driving module is at least one permanent magnet, and the sample processing module 3 is fixedly arranged between the south pole and the north pole of the at least one permanent magnet. The implementation is simple and easy to implement, and the cost is low.

[0082] Further, the angle between the magnetic field direction of the magnetic field driving module and the plane where the contact layer of the layered processing unit is located is greater than or equal to 60 degrees. Such a magnetic field direction can ensure that the adsorbent is peeled off by fatigue failure in the contact layer, thereby achieving the purpose of strengthening the separation of different particles in the sample, and the contact layer of the layered processing unit is appropriately inclined downward, which is more convenient for the collection of waste liquid due to the action of gravity.

[0083] Further, the magnetic field driving module is provided with a first linear Hall sensor and a first linear Hall sensor. In this way, the magnetic field strength and direction can be monitored, and adjustments can be made accordingly to adapt to different scene requirements.

[0084] Further, the first coil and the second coil of the Helmholtz coil have the same size; according to the principle of magnetic field superposition, when two coils of the same size are supplied with currents in the same direction, the magnetic fields generated by the two coils at a certain point on the axis will superimpose each other, thus forming a stronger magnetic field on the axis. When the two coils are supplied with currents in opposite directions, the magnetic fields generated by the two coils will cancel each other out, thus forming a region on the axis where the magnetic field is zero. The same size of the two coils ensures the symmetry of the Helmholtz coil, which is very important for generating a uniform magnetic field. The symmetry ensures the uniform distribution of the magnetic field in the central region of the two coils. And because the size and number of turns of the two coils are the same, changing the current intensity and direction through them can conveniently adjust the strength and direction of the generated magnetic field,

[0085] Further, the distance between the centers of the first coil and the second coil is equal to the radius of the first coil; in this particular configuration, the superposition of the magnetic fields generated by the two coils in their central regions can achieve maximum uniformity. This is because the gradient of the magnetic fields generated by the two coils in the central region is minimal, thus making the magnetic field distribution more uniform. The design of the distance between the centers of the two coils equal to the radius of the coil ensures the symmetry of the magnetic field. This symmetry helps to reduce the non-uniformity of the magnetic field, as the magnetic fields generated by the two coils in the central region cancel out the gradient effect of the other. In this configuration, the magnetic field intensity in the central region of the two coils can reach a maximum. This is because the superposition effect of the magnetic fields generated by the two coils in the central region is optimal, thus maximizing the magnetic field intensity.

[0086] Further, the first coil and the second coil are wound in the same direction; by winding the two coils in the same direction and supplying them with currents in the same direction, precise control of the magnetic field can be achieved. This configuration allows the strength and direction of the generated magnetic field to be adjusted by changing the size and direction of the current, meeting the needs of different experiments and applications.

[0087] Further, the first coil and the second coil are wound in the same direction; by winding the two coils in the same direction and supplying them with currents in the same direction, precise control of the magnetic field can be achieved. This configuration allows the strength and direction of the generated magnetic field to be adjusted by changing the size and direction of the current, meeting the needs of different experiments and applications.

[0088] Further, the distance from the first linear Hall sensor to the center of the first coil is equal to the distance from the first linear Hall sensor to the center of the sample processing module 3; ensuring maximum accuracy and consistency in measuring the magnetic field.

[0089] Further, the sample processing module 3 is detachable; this allows the sample processing instrument to use different sample processing modules 3 with different parameters for different samples, thus making the sample processing more targeted and effective.

[0090] Further, the sample processing instrument comprises at least two sample processing modules 3, the parameters of the at least two sample processing modules 3 are different, and the at least two sample processing modules 3 are connected by pipelines. After the sample front sample flows through one of the sample processing modules 3, it can flow through another sample processing module 3. After the sample front sample passes through the at least two sample processing modules 3, it can meet more complex sample processing requirements. Here, the parameters of the sample processing module 3 include the pore size and thickness of the contact layer of the layered processing unit.

[0091] Further, the sample processing instrument can also comprise at least one sample processing module 3 or at least one sample separation module. The sample processing module 3 mainly processes the sample front sample through the layered processing unit, while the sample separation module mainly separates various particles of different properties in the sample front sample through gel filtration chromatography, ion exchange chromatography, hydrophobic chromatography and affinity chromatography. Since the particle separation methods of the sample processing module 3 and the sample separation module are different, they can meet more complex sample processing requirements.

[0092] Further, the bidirectional driving module is a peristaltic pump. The working principle of the peristaltic pump is that the rollers extrude the hose to form a self-suction pressure, suck the liquid into the hose, and transport the liquid to the liquid outlet end with the rotation of the rollers. The peristaltic pump mainly consists of a driver, a pump head and a hose. The fluid is isolated in the pump pipe and only contacts with the hose, not directly with the pump body. Therefore, it has the characteristics of no pollution, low shear force and easy cleaning, so it will not cause the phenomenon of particle (such as protein) aggregation or entanglement.

[0093] Further, the sample processing post-chamber 311 or the third port 343 is connected with a flow monitoring module. The flow monitoring module can monitor the flow of the sample post sample flowing out of the sample processing post-chamber 311 or the third port 343, while the processing amount of the sample front sample is generally known in advance, so that the flow of the remaining part of the sample front sample can be obtained, and the flow in and out of the whole liquid path system can be accurately obtained.

[0094] Further, the sample processing pre-chamber 344 or the first port 341 or the second port 342 is connected with a parameter measuring module. Here, the parameters are various parameters of the sample front sample, such as PH value, UV value, etc. In this way, the state of the remaining sample front sample after sample processing can be obtained.

[0095] Referring to Figure 4 , Figure 4 is one of the structure schematic diagrams of an embodiment of the sample detection device of the present application, as Figure 4 Further, the plane where the contact layer of the layered processing unit is located is perpendicular to the ground or inclined upward. Figure 4The side view of the flow channel baffle unit and the layered treatment unit is described. The cross-membrane pressure is further increased due to the effect of gravity, and the waste liquid is more convenient to collect.

[0096] Further, the pore size of the contact layer of the layered treatment unit is smaller than the pore size of other layers of the layered treatment unit; thus the filtration function of the layered treatment unit for particles in the sample pre-sample is better.

[0097] Further, the thickness of the contact layer of the layered treatment unit is smaller than the thickness of other layers of the layered treatment unit. Thus the layered treatment unit is an asymmetric membrane, which is usually composed of a thin selective layer and a thick support layer. This structure makes the membrane have higher retention performance and better filtration function for particles in the sample pre-sample.

[0098] Further, the layered treatment unit includes a first layered treatment unit 33 and a second layered treatment unit 35. The area of the layered treatment unit is larger, and the flux of the sample pre-sample that can be processed is higher.

[0099] Referring to Figure 2 , Figure 2 is one of the structural schematic diagrams of an embodiment of the sample detection device of the present application, as Figure 2 shown, the sample processing module 3 further includes a first sealing elastic unit 32, a second sealing elastic unit 36, a first non-elastic cover unit 31, a second non-elastic cover unit 37 and a fixing structure 38. The fixing structure 38 fixes the first non-elastic cover unit 31, the first sealing elastic unit 32, the first layered treatment unit 33, the flow channel baffle unit 34, the second layered treatment unit 35, the second sealing elastic unit 36 and the second non-elastic cover unit 37 in sequence. Here, the fixing structure 38 can be that the first non-elastic cover unit 31, the first sealing elastic unit 32, the first layered treatment unit 33, the flow channel baffle unit 34, the second layered treatment unit 35, the second sealing elastic unit 36 and the second non-elastic cover unit 37 all have fixing through holes, and screws pass through the through holes and are fixed together with nuts and screw rods. This fixing method is simple to install, low in cost and has good sealing effect.

[0100] In another embodiment, the sample processing module 3 does not necessarily include the first sealing elastic unit 32 and the second sealing elastic unit 36. If the separation membrane is a PVDF or PES membrane, it can be directly fixed on the non-elastic cover unit or the flow channel baffle unit (preferably directly fixed on the flow channel baffle unit, so that if the sample processing module is replaced, only the flow channel baffle unit can be replaced, further reducing maintenance cost) by using ultrasonic welding process; if the separation membrane is a ceramic membrane, it can only be pressed by the sealing elastic unit.

[0101] Referring to Figure 5 , Figure 5This is a schematic diagram of one embodiment of the sample detection device of this application, as shown below. Figure 5 As shown, the first sealing elastic unit 32 is provided with at least one first positioning hole 321, and the first non-elastic sealing cap unit 31 or the flow channel baffle unit 34 is provided with at least one first positioning post 345 that cooperates with at least one first positioning hole 321. At least one first positioning hole 321 corresponds to at least one first positioning post 345. Since the sealing elastic unit is made of elastic material, it can be a sealing gasket and is easy to open. Therefore, it is easy to deform and shift during sealing and fixing installation. With the cooperation of positioning hole 321 and positioning post 345, it is not easy to shift, and the sealing effect is better.

[0102] Furthermore, the second sealing elastic unit 36 ​​is provided with at least one second positioning hole 321, and the second non-elastic sealing cap unit 37 or the flow channel baffle unit 34 is provided with at least one second positioning post 345 that cooperates with at least one second positioning hole 321, with each second positioning hole 321 corresponding to at least one second positioning post 345. Similarly, since the sealing elastic unit is made of elastic material, it can be a sealing gasket, which is easy to drill. Therefore, it is easy to deform and shift during sealing and fixing installation. With the cooperation of the positioning hole 321 and the positioning post 345, it is not easy to shift, and the sealing effect is better.

[0103] Furthermore, the post-processing cavity 311 includes a first post-processing cavity 311 and a second post-processing cavity 311; this means that the layered processing unit includes a first layered processing unit 33 and a second layered processing unit 35, and the layered processing unit has a larger area and can process a higher throughput of pre-processed samples.

[0104] Furthermore, the first sealing elastic unit 32 is provided with a first empty groove, and the first non-elastic sealing cap unit 31 is provided with a first groove. The cavity formed by the first groove, the first empty groove and the first layered processing unit 33 together is the first sample processing cavity 311. Generally, the larger the volume of the sample processing cavity 311, the more stable the pressure in the sample processing cavity 311, and the more stable the transmembrane pressure on both sides of the layered processing unit, which is more conducive to the precise control of the sample processor.

[0105] Furthermore, the second sealing elastic unit 36 ​​is provided with a second empty groove, and the second non-elastic sealing cap unit 37 is provided with a second groove. The cavity formed by the second groove, the second empty groove, and the second layered processing unit 35 is the second sample processing cavity 311. Similarly, the larger the volume of the sample processing cavity 311, the more stable the pressure in the sample processing cavity 311, and the more stable the transmembrane pressure on both sides of the layered processing unit, which is more conducive to the precise control of the sample processor.

[0106] Further, the first port 341, the second port 342 and the third port 343 are arranged on the flow channel baffle unit 34, which is more convenient for processing and helps to reduce the processing cost of the sample processing module 3 while ensuring a certain precision.

[0107] Further, the third port 343 of the flow channel baffle unit 34 is also connected with a horizontal through hole, so that the flow channel baffle unit 34 is more convenient for processing and helps to reduce the processing cost of the sample processing module 3 while ensuring a certain precision.

[0108] Further, the first recess on the first non-elastic cover unit 31 is connected with the third port 343 through a horizontal through hole, and the second recess on the second non-elastic cover unit 37 is connected with the third port 343 through a horizontal through hole; in this way, the flow channel baffle unit 34 is more convenient for processing and helps to reduce the processing cost of the sample processing module 3 while ensuring a certain precision.

[0109] Further, the third port 343 is arranged downward; in this way, the replacement and installation of the sample processing module 3 can be easily completed by simply inserting.

[0110] Further, the third port 343 is arranged downward, and the first port 341, the second port 342 and the third port 343 are arranged on the same side of the flow channel baffle unit 34, and the first port 341, the second port 342 and the third port 343 are located on the same straight line, so that the installation precision of the first port 341, the second port 342 and the third port 343 can be easily ensured at the same time, and the overall installation precision and sealing performance of the sample processing module 3 can be ensured.

[0111] Referring to Figure 6 , Figure 6 is one of the structural schematic diagrams of an embodiment of the sample detection device of the present application, as Figure 6 shown, further, the horizontal cross-sectional area of the third port 343 near the port gradually decreases from high to low, so that the waste liquid in the sample processing rear cavity 311 gradually shrinks near the port due to the action of gravity, and is more easily discharged due to negative pressure or other driving action.

[0112] Further, when the sample front sample flows through the sample processing front cavity 344, the area of the contact layer that can be contacted is the contact area, and the ratio of the contact area to the volume of the sample processing front cavity 344 is greater than 10 6 / m. In the case that the volume of the sample processing front cavity 344 is relatively constant, the larger the area of the contact layer that can be contacted by the sample front sample flowing through the sample processing front cavity 344, the higher the efficiency and flux of the sample front sample obtained by processing, and with such a proportion, it is more suitable for the scene of sample front sample processing.

[0113] In the description of the application, the description of the terms "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the application. In the description of the application, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, the person skilled in the art can combine and combine the different embodiments or examples described in the specification and the features of the different embodiments or examples without contradiction and in combination.

[0114] In addition, the terms "first", "second" are only for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined with "first", "second" can explicitly or implicitly include at least one of the features. In the description of the application, the meaning of "multiple" is at least two, for example, two, three, etc., unless otherwise specifically limited.

[0115] Any process or method descriptions in flow charts or described elsewhere herein can be understood as representing code modules, segments, or portions of code that include one or more executable instructions for performing specific logic functions or steps in the process, and the various embodiments of the application include additional implementations in which the order of steps can be changed, including use of an opposite order, and additional or fewer steps can be performed, depending on the functionality involved as would be understood by one skilled in the art. The various embodiments of the application can be implemented in hardware, software, or a combination thereof.

[0116] The above description is only the implementation of the application, and does not limit the patent scope of the application, and any equivalent structure or equivalent process transformation using the content of the specification and drawings, or direct or indirect application in other related technical fields, are also included in the patent protection scope of the application.

Claims

1. A sample processing instrument, characterized by, The sample processing module comprises a first port, a second port, a third port, a sample processing cavity, a flow channel baffle unit and at least one layered processing unit. The sample processing module comprises a first port, a second port, a third port, a sample processing cavity, a flow channel baffle unit and at least one layered processing unit, The layered processing unit is a multi-layer structure, which divides the sample processing cavity into at least one sample pre-processing cavity and at least one sample post-processing cavity, and one layer of the multi-layer structure of the layered processing unit in contact with the sample pre-processing cavity is a contact layer, The flow channel baffle unit divides the sample pre-processing cavity into at least one sample flow channel unit, The first port and the second port are in communication with at least one sample flow channel unit, The third port is in communication with the sample post-processing cavity; The sample processing instrument obtains a sample pre-sample, and the bidirectional driving module drives the sample pre-sample to flow into the sample pre-processing cavity through the first port, Or, flow out of the sample pre-processing cavity through the second port, Or, flow into the sample pre-processing cavity through the second port, Or, flow out of the sample pre-processing cavity through the first port; Part of the sample pre-sample flows into the sample post-processing cavity after passing through the layered processing unit to become a sample post-sample, and the sample post-sample flows out of the third port; The sample pre-processing cavity does not have a direct driving device, and the bidirectional driving module, the indirect driving module and the non-contact driving module are all indirect driving devices.

2. The sample processing instrument according to claim 1, wherein The contact layer is in a plane, the indirect driving module is in fixed contact with the contact layer of the layered processing unit, And / or, the indirect driving module is a mechanical vibration driving module; And / or, the mechanical vibration frequency range of the mechanical vibration driving module is 5Hz-30KHz; And / or, the non-contact driving module is a magnetic field driving module; And / or, the angle between the magnetic field direction of the magnetic field driving module and the plane in which the contact layer of the layered processing unit is located is greater than or equal to 60 degrees.

3. The sample processing instrument according to claim 2, wherein The magnetic field driving module is a Helmholtz coil, and the sample processing module is fixedly arranged between a first coil and a second coil of the Helmholtz coil, Or, the magnetic field driving module is at least one permanent magnet, and the sample processing module is fixedly arranged between the south pole and the north pole of the at least one permanent magnet; And / or, the magnetic field driving module is provided with a first linear Hall sensor and a first linear Hall sensor.

4. The sample processing instrument according to claim 3, wherein The first coil and the second coil of the Helmholtz coil are the same size; And / or, the distance between the center of the first coil and the second coil is equal to the radius of the first coil; And / or, the first coil and the second coil are wound in the same direction; And / or, the current directions of the first coil and the second coil are the same. And / or, the distance from the first linear Hall sensor to the center of the first coil is equal to the distance from the first linear Hall sensor to the center of the sample processing module.

5. The sample processing instrument of claim 1, wherein, the sample processing module is detachable; and / or, the sample processing instrument comprises at least two sample processing modules, and the parameters of the at least two sample processing modules are different, or, the sample processing instrument comprises at least one sample processing module or at least one sample separation module.

6. The sample processing instrument of claim 1, wherein, the bidirectional driving module is a peristaltic pump; and / or, the sample processing post-chamber or the third port is connected with a flow monitoring module; and / or, the sample processing pre-chamber or the first port or the second port is connected with a parameter measuring module.

7. The sample processing instrument of claim 1, wherein, the plane of the contact layer of the layered processing unit is perpendicular to the ground or inclined upward; characterized in that and / or, the pore size of the contact layer of the layered processing unit is smaller than that of other layers of the layered processing unit; and / or, the thickness of the contact layer of the layered processing unit is smaller than that of other layers of the layered processing unit.

8. The sample processing instrument of claim 1, wherein, the layered processing unit comprises a first layered processing unit and a second layered processing unit, the sample processing module further comprises a first sealing elastic unit, a second sealing elastic unit, a first non-elastic cover unit, a second non-elastic cover unit and a fixing structure, the fixing structure sequentially fixes the first non-elastic cover unit, the first sealing elastic unit, the first layered processing unit, the flow channel baffle unit, the second layered processing unit, the second sealing elastic unit and the second non-elastic cover unit together; and / or, the first sealing elastic unit is provided with at least one first positioning hole, and the first non-elastic cover unit or the flow channel baffle unit is provided with at least one first positioning column matched with the at least one first positioning hole, the at least one first positioning hole and the at least one first positioning column corresponding one by one; and / or, the second sealing elastic unit is provided with at least one second positioning hole, and the second non-elastic cover unit or the flow channel baffle unit is provided with at least one second positioning column matched with the at least one second positioning hole, the at least one second positioning hole and the at least one second positioning column corresponding one by one.

9. The sample processing instrument of claim 8, wherein, the sample processing post-chamber comprises a first sample processing post-chamber and a second sample processing post-chamber; and / or, the first sealing elastic unit is provided with a first empty groove, the first non-elastic cover unit is provided with a first recess, and the first recess, the first empty groove and the first layered processing unit together form a first sample processing post-chamber. ​ ​ ​ And / or, the second sealing elastic unit is provided with a second hollow groove, the second non-elastic cover unit is provided with a second recess, and the cavity formed by the second recess, the second hollow groove and the second layered processing unit is a second sample processing rear cavity; And / or, the first port, the second port and the third port are all arranged on the flow channel baffle unit; And / or, the third port of the flow channel baffle unit is also connected with a horizontal through hole; And / or, the first recess on the first non-elastic cover unit is connected with the third port through the horizontal through hole; And / or, the second recess on the second non-elastic cover unit is connected with the third port through the horizontal through hole; And / or, the third port is arranged downward; And / or, the horizontal cross-sectional area of the third port near the port gradually decreases from high to low.

10. A sample processing instrument according to claim 1, characterized in that, The ratio of the area of the contact layer accessible to the sample pre-flow as it passes through the sample processing pre-chamber to the volume of the sample processing pre-chamber is greater than 10 6 / m.

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