Cell cryopreservation bag
By incorporating an air inlet tube and a cavity connection structure within the cell cryopreservation bag, the problem of uneven cell suspension dispensing was solved, enabling automated uniform dispensing and efficient cryopreservation.
Patent Information
- Application Number
- CN202321770446.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2023-07-06
- Publication Date
- 2026-01-23
- Estimated Expiration
- 2033-07-06
AI Technical Summary
Existing multi-chamber cryopreservation bags have difficulty achieving uniform dispensing of cell suspensions, resulting in uneven cell suspension volumes in each chamber and affecting the quality of test results.
Design a cell cryopreservation bag containing multiple interconnected cavities and an air injection tube. By connecting each cavity to the outside atmosphere after injection, consistent air pressure is ensured, thereby automatically and evenly dispensing the cell suspension into each cavity.
It enables automated and uniform dispensing of cell suspension samples, reducing manual operations, shortening time, and improving the efficiency of cell cryopreservation and the accuracy of detection.
Smart Images
Figure CN223816834U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the field of biological medical devices, in particular to a cell cryopreservation bag. BACKGROUND
[0002] Cell cryopreservation is one of the effective methods for long-term preservation of cells, which is a technology that places cells in a low-temperature environment to reduce cell metabolism for long-term storage. Using cell cryopreservation technology can preserve cells and prevent contamination or loss.
[0003] At present, the cryopreservation carrier container for loading cell products is mostly a multi-cavity cryopreservation bag. However, the multi-cavity cryopreservation bag on the market is difficult to uniformly distribute the cell suspension into each cavity when distributing the sample, resulting in uneven volume of cell suspension in each cavity, which affects the quality of subsequent detection results. CONTENT OF THE UTILITY MODEL
[0004] Therefore, the present application provides a cell cryopreservation bag which can uniformly distribute the cell suspension into each cavity.
[0005] A cell cryopreservation bag includes a bag body, a sample inlet portion, and a sample outlet portion. The bag body includes a plurality of cavities in communication, and a channel between adjacent cavities to communicate the adjacent cavities. The sample inlet portion includes a sample inlet and an air inlet tube connected to the sample inlet, and the sample inlet is arranged on the bag body and communicates with one of the cavities. The air inlet tube is used to connect the external atmosphere, so that the air pressure in each cavity is consistent with the external atmospheric pressure. The sample outlet portion is arranged on the bag body, and the sample outlet portion has a plurality of sample outlet portions, one sample outlet portion corresponding to one cavity.
[0006] In one embodiment, the cell cryopreservation bag has a first state and a second state. When the cell cryopreservation bag is in the first state, the channel communicates adjacent cavities. When the cell cryopreservation bag is in the second state, the channel is closed, and the adjacent cavities are not in communication.
[0007] In one embodiment, a strip-shaped slit is arranged between adjacent cavities. The strip-shaped slit is used to separate adjacent cavities, and the channel is located at both ends of the strip-shaped slit.
[0008] In one embodiment, the cell cryopreservation bag also has a third state. When the cell cryopreservation bag is in the third state, adjacent cavities are separated along the strip-shaped slit, and each cavity remains independent.
[0009] In one embodiment, a filter is arranged on the air inlet tube, and a luer cap is arranged on the filter.
[0010] In one embodiment, the air inlet tube is further provided with a pinch valve, which is arranged between the filter and the inlet port.
[0011] In one embodiment, each of the sampling portions comprises a sampling port and a soft cover sealing the sampling port, the sampling port being in communication with the cavity, and the soft cover being configured to be removed to expose the sampling port.
[0012] In one embodiment, the sampling portion further comprises a filling conduit connected to the inlet port, the filling conduit satisfying at least one of the following conditions:
[0013] The filling conduit is provided with a rubber plug port, which is configured to be pierced by a needle of a syringe to inject a sample in the syringe into the inlet port and into the cavity;
[0014] The filling conduit is provided with a luer joint, which is configured to be connected to a syringe to inject a sample in the syringe into the inlet port and into the cavity.
[0015] In one embodiment, the luer joint is a male luer joint, and the filling conduit is provided with a pinch valve on a side close to the male luer joint.
[0016] In one embodiment, the luer joint is a female luer joint, and the filling conduit is provided with a pinch valve on a side close to the female luer joint.
[0017] The cell cryopreservation bag of the present application can make each cavity communicate with the atmosphere after the sampling is completed by arranging an air inlet tube in the sampling portion and arranging a channel between each cavity, so that the cell suspension sample can be automatically and uniformly distributed into each cavity, improving the uniformity of the cell suspension sample distribution, and without the need for manual squeezing by the operator, which is convenient and shortens the time, improving the efficiency of cell cryopreservation. BRIEF DESCRIPTION OF DRAWINGS
[0018] Figure 1 The structure schematic diagram of the cell cryopreservation bag provided for an embodiment of the prior art.
[0019] Figure 2 The structure schematic diagram of the cell cryopreservation bag provided for an embodiment of the present application.
[0020] Figure 3 The structure schematic diagram of the cell cryopreservation bag provided for Figure 2 The structure schematic diagram of the cell cryopreservation bag during sampling.
[0021] Figure 4 The structure schematic diagram of the cell cryopreservation bag after heat sealing. Figure 2 The structure schematic diagram of the cell cryopreservation bag after heat sealing.
[0022] Figure 5 Fig. 4 is a schematic view of the structure of the cell freezing bag shown in Fig. 1 after the bag is cut into individual cavities. Figure 4 Fig. 5 is a schematic view of the structure of the cell freezing bag shown in Fig. 1 after the bag is cut into individual cavities.
[0023] Figure 6 Fig. 6 is a schematic view of the structure of the cell freezing bag shown in Fig. 1 after the bag is cut into individual cavities. Figure 5 Fig. 7 is a schematic view of the structure of the cell freezing bag shown in Fig. 1 after the bag is cut into individual cavities.
[0024] Explanation of Main Elements
[0025] Cell freezing bag 1
[0026] Cavity 2
[0027] Sample inlet 3
[0028] Sample outlet 4
[0029] Strip-shaped slit 5
[0030] Passage 6
[0031] Cell freezing bag 100
[0032] Bag body 10
[0033] Sample inlet 20
[0034] Sample outlet 30
[0035] Filling conduit 40
[0036] Cavity 11
[0037] Passage 12
[0038] Heat-sealed portion 121
[0039] Strip-shaped slit 13
[0040] Sample inlet 21
[0041] Hose 211
[0042] Air inlet tube 22
[0043] Filter 23
[0044] Luer cap 24
[0045] Sample outlet 31
[0046] Soft cap 32
[0047] Rubber stopper 41
[0048] Luer fitting 42
[0049] Male luer fitting 421
[0050] Female luer fitting 422
[0051] Luer screw cap 423
[0052] Syringe 43
[0053] Pinch valve 44
[0054] The following detailed description, in conjunction with the accompanying drawings, further illustrates the embodiments of this application. Detailed Implementation
[0055] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which embodiments of this application belong. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the embodiments of this application.
[0056] It should be noted that all directional indicators (such as up, down, left, right, front, back, etc.) in the embodiments of this application are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicator will also change accordingly.
[0057] Furthermore, the use of terms such as "first" and "second" in this application is for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.
[0058] Please see Figure 1 The illustration shows a prior art cell cryopreservation bag 1, which may include multiple cavities 2, an inlet 3, and an outlet 4. Each cavity 2 is relatively independent, and a strip slit 5 is provided between adjacent cavities 2. The strip slit 5 is mainly used to separate two adjacent cavities 2, making each cavity 2 relatively independent. Before sample injection, adjacent cavities 2 are connected, thus allowing all cavities 2 to be connected, and allowing the sample (e.g., cell suspension) to flow from the inlet 3 into each cavity 2. Specifically, as shown... Figure 1 As shown, at the lower end of cavity 2, adjacent cavities 2 can be connected through a narrow channel 6, allowing cell suspension to flow from the inlet 3 through the channel 6 into each cavity 2, ensuring that each cavity 2 contains cell suspension. After injection, the inlet 3 and channel 6 can be heat-sealed, ultimately making each cavity 2 completely independent.
[0059] However, in the process of sample injection, the inside of the cell freezing bag 1 is completely closed, and the pressure inside the cell freezing bag 1 increases with the increase of the amount of cell suspension, and finally is in an inflated state, and the sample in the bag cannot be automatically and uniformly distributed to each cavity 2. Especially when the amount of sample added is small, the amount of cell suspension in each cavity 2 is more different, and manual operation is required to adjust to make the volume of cell suspension in each cavity 2 as consistent as possible.
[0060] Therefore, the present application proposes a cell freezing bag 100 to solve the technical problem that the existing cell freezing bag 1 cannot automatically and uniformly distribute the sample (cell suspension) to each cavity.
[0061] Some embodiments of the present application will be described in detail below with reference to the accompanying drawings. The following embodiments and features in the embodiments can be combined with each other without conflict.
[0062] Please refer to Figure 2 The present application proposes a cell freezing bag 100, which comprises a bag body 10, a sample injection part 20 and a sample taking part 30. The shape of the bag body 10 is not limited as long as it is convenient for loading samples. The sample can be but is not limited to cell suspension, and the cell suspension can be but is not limited to mesenchymal stem cells, hematopoietic stem cells, immune cells, neural stem cells or endothelial progenitor cells of various tissue sources. The bag body 10 can be made of light, thin, soft, transparent and low-temperature long-term storage materials such as EVA (ethylene-vinyl acetate copolymer) or other materials that can be used for low-temperature storage. The inside of the bag body 10 comprises a plurality of communicating cavities 11, and any two adjacent cavities 11 have a channel 12 to communicate the adjacent cavities 11, and the volume of each cavity 11 is substantially equal. In this embodiment, the number of cavities 11 is 5, and in other embodiments, the number of cavities 11 can also be adjusted according to needs. As shown in Figure 2 The five cavities 11 are arranged along the horizontal direction, and the channels 12 are located at both ends of the cavities 11 in the vertical direction. That is, in the cell freezing bag 100 shown in Figure 2 In the cell freezing bag 100 shown in
[0063] As shown in Figure 2As shown, the sample injection section 20 includes an inlet 21 and an air injection tube 22 connected to the inlet 21. The inlet 21 is located on the bag body 10 and communicates with one of the cavities 11. The inlet 21 may be located at the lower end of the bag body 10. The inlet 21 may protrude from the outer surface of the bag body 10 and extend a certain distance from the outer surface of the bag body 10. A flexible tube 211 may be connected to the outside of the inlet 21. The inlet 21 and the flexible tube 211 may be integrally formed (fixedly connected) with the bag body 10. The inlet 21 is used to inject the cell suspension into the cavity 11 connected to the inlet 21 and allow it to flow into other cavities 11. The air injection tube 22 is connected to the flexible tube 211 and is connected to the inlet 21 through the flexible tube 211. The air injection tube 22 is used to connect to the outside atmosphere (the outside refers to the outside of the cell cryopreservation bag 100) to ensure that the air pressure in each cavity 11 is consistent with the outside atmospheric pressure.
[0064] like Figure 2 As shown, a sampling section 30 is disposed on the bag body 10, and the sampling section 30 can be integrally formed (fixedly connected) with the bag body 10. There are multiple sampling sections 30, with one sampling section 30 corresponding to one cavity 11. In this embodiment, there are 5 cavities 11, and correspondingly, there are also 5 sampling sections 30. The sampling section 30 can be disposed at the upper end of the bag body 10. The sampling section 30 is used for a sampler (e.g., a syringe) to take samples from the cavity 11 for subsequent testing, etc.
[0065] After the sample injection operation is completed, the air injection tube 22 is opened, allowing outside air to enter the injection port 21 through the air injection tube 22, and then into the cavity 11 connected to the injection port 21. Since each cavity 11 is connected through the channel 12, each cavity 11 is open to the outside atmosphere, meaning the pressure inside each cavity 11 will be consistent with the atmospheric pressure. This allows the cell suspension to automatically flow into each cavity 11 through the channel 12, maintaining a consistent liquid level in each cavity 11, thus ensuring that the volume of cell suspension in each cavity 11 is the same. The cell cryopreservation bag 100 described in this application can automatically and uniformly dispense cell suspension samples into each cavity 11, improving the uniformity of cell suspension sample dispensing. It also eliminates the need for manual squeezing by the operator, facilitating operation, shortening time, and improving the efficiency of cell cryopreservation.
[0066] like Figure 2 As shown, in some embodiments, a strip-shaped slit 13 is provided between adjacent cavities 11. The strip-shaped slit 13 is used to separate adjacent cavities 11, and the channel 12 is located at both ends of the extending direction of the strip-shaped slit 13. In this embodiment, the extending direction of the strip-shaped slit 13 is the length direction of the strip-shaped slit 13, that is... Figure 2 In the vertical direction, channel 12 is located at the upper and lower ends of strip slit 13.
[0067] likeFigure 2 As shown, in some embodiments, the air injection tube 22 is equipped with a filter 23, and the filter 23 is equipped with a Luer cap 24. The filter 23 is used to filter out dust and other impurities in the air to prevent contamination of the cell suspension. In the initial state, the Luer cap 24 on the filter 23 is in the tightened state, and the cavity 11 is isolated from the outside (not connected). After the injection operation is completed, the Luer cap 24 is opened, and the outside air is filtered by the filter 23 and enters the cavity 11, so that the pressure in each cavity 11 is consistent with the atmospheric pressure.
[0068] like Figure 2 As shown, in some embodiments, the air inlet tube 22 is also provided with a clamp valve 25. The clamp valve 25 is located between the filter 23 and the inlet port 21 and can be used to clamp and close the air inlet tube 22. In some embodiments, the clamp valve 25 may also be omitted.
[0069] like Figure 3 As shown, in some embodiments, each sampling unit 30 includes a sampling port 31 and a soft cap 32 sealing the sampling port 31. The sampling port 31 communicates with the cavity 11, and the soft cap 32 is configured to be removable to expose the sampling port 31. When sampling is required, the soft cap 32 can be unscrewed by rotation to expose the sampling port 31.
[0070] like Figure 3 As shown, the sample inlet 20 also includes a filling conduit 40 connected to the sample inlet 21. The filling conduit 40 is connected to the side of the tubing 211 away from the sample inlet 21, and the filling conduit 40 can be integrally formed with the sample inlet 21 and the tubing 211. The material of the filling conduit 40 can be the same as that of the tubing 211, and the filling conduit 40 can be regarded as an extension of the tubing 211. The connection point of the air injection tube 22 on the tubing 211 can be regarded as the boundary between the filling conduit 40 and the tubing 211, such as... Figure 3 As shown, the left side of the hose 211 is the filling conduit 40, and the right side is the hose 211.
[0071] In some embodiments, such as Figure 3 As shown, the filling catheter 40 is provided with a rubber stopper 41, which can be a puncturable rubber stopper. The puncturable rubber stopper can be punctured by the needle of a syringe, and the cell suspension sample in the syringe can be injected into the injection port 21 through the punctured rubber stopper 41 and enter the cavity 11.
[0072] In some embodiments, such as Figure 3 As shown, the filling tubing 40 is equipped with a Luer connector 42. The Luer connector 42 is configured to connect to a syringe 43 to inject a cell suspension sample from the syringe 43 into the inlet 21 and into the cavity 11. When no injection is required, the Luer connector 42 can be tightened and sealed by a Luer screw cap 423.
[0073] Specifically, the Luer connector 42 can be a male Luer connector 421. A clamp valve 44 can be provided on the side of the filling conduit 40 near the male Luer connector 421 to clamp and close the conduit, making it easy to stop the sample injection.
[0074] Specifically, the Luer connector 42 can be a female Luer connector 422. The filling conduit 40 is provided with a clamp valve 44 on the side near the female Luer connector 422 to clamp and close the conduit, making it easy to stop the sample injection.
[0075] Those skilled in the art will understand that the filling conduit 40 may be equipped with a rubber stopper 41, a male Luer connector 421, and a female Luer connector 422, so that the operator can flexibly select the appropriate injection method.
[0076] The following will provide a detailed explanation of how to use the cell cryopreservation bag 100.
[0077] First, take out... Figure 3 The cell cryopreservation bag 100 shown is in its normal state, that is, before sample injection and before use, which is referred to as the first state in this application. In the first state, the channel 12 is connected to the adjacent cavity 11. The filling tubing 40 and the air injection tube 22 are connected to the injection port 21. At this time, the clamp valves 25 and 44 on each pipeline are closed, the Luer cap 24 on the filter 23 is tightened, and the Luer cap 423 on the Luer connector 42 is tightened.
[0078] Next, as Figure 4 As shown, a cell suspension sample can be drawn using a syringe 43. The sample can be injected through the Luer connector 42 or the rubber stopper 41. Unscrew the Luer cap 423 on the Luer connector 42 (male Luer connector 421 or female Luer connector 422), connect the open end of the syringe 43 to the Luer connector 42, and inject the cell suspension sample in the syringe 43 into the cavity 11 through the injection port 21. After injection, tighten the Luer cap 423 to seal the Luer connector 42. Alternatively, the needle of the syringe 43 can be used to puncture the rubber stopper 41, and the cell suspension sample in the syringe 43 can be injected into the injection port 21 and into the cavity 11.
[0079] After the injection is complete, syringe 43 can be removed. Then, lift the cell cryopreservation bag 100 so that the sampling section 30 is at the bottom and the injection port 21 is at the top, with the cell cryopreservation bag 100 roughly perpendicular to the horizontal plane. Lift the tubing 211 at the injection port 21 so that the tubing 211 is higher than the height of the injection port 21.
[0080] Next, open the Luer cap 24 on the filter 23 to allow air from the atmosphere to pass through the filter 23 and enter the cavity 11, thereby automatically adjusting the liquid level of the cell suspension in the cavity 11 to a uniform level.
[0081] Next, the tubing 211 at the sample inlet 21 can be sealed using a heat sealer, such as... Figure 4 As shown. At this time, the air inlet tube 22 and filling conduit 40 on the left side of the heat-sealed part of the tubing 211 have been disconnected from the tubing 211. After the tubing 211 is heat-sealed, the cell cryopreservation bag 100 remains perpendicular to the horizontal plane, and all channels 12 between the cavities 11 are heat-sealed using a heat-sealing machine. It can be understood that the channels 12 are closed after heat sealing, forming a heat-sealed part 121. The heat-sealed part 121 is a solid part, which is sealed to the strip slit 13. The heat-sealed part 121 and the strip slit 13 together block each cavity 11, making the adjacent cavities 11 not connected, and each cavity 11 is in a completely independent state. This is the second state of the cell cryopreservation bag 100. Figure 5 The heat-sealed area is indicated in dark black.
[0082] After heat sealing, the cell cryopreservation bag 100 can be cut open along the heat-sealed part 121 and the strip seam 13 with scissors to obtain the following: Figure 6 The individual cavities 11 shown (each cavity 11 has a sampling section 30) remain completely independent of each other. This is the third state of the cell cryopreservation bag 100 (i.e., the heat-sealed cell cryopreservation bag 100 is cut into multiple individual cavities 11). Each independent cavity 11 can be cryopreserved independently without affecting each other, which facilitates subsequent sampling and testing.
[0083] When sampling and testing are required, such as As shown, the soft cover 32 of the sampling section 30 can be rotated and unscrewed to expose the sampling port 31. The sampling port 31 can be punctured with the needle of a syringe, and then the cell suspension can be drawn for sampling and testing.
[0084] The cell cryopreservation bag 100 of this application, by setting an air injection tube 22 in the sample injection section 20 and setting a channel 12 between each cavity 11, allows each cavity 11 to be connected to the outside atmosphere after sample injection, thereby enabling the cell suspension sample to be automatically and evenly dispensed into each cavity 11, improving the uniformity of cell suspension sample dispensing, eliminating the need for manual squeezing by the operator, facilitating operation, shortening time, and improving the efficiency of cell cryopreservation.
[0085] The above description describes some specific embodiments of this application, but in actual applications, the application should not be limited to these embodiments. For those skilled in the art, other modifications and alterations made based on the technical concept of this application should fall within the protection scope of this application.
Claims
1. A cell cryopreservation bag, characterized in that, include: The bag body includes a plurality of interconnected cavities, with channels between adjacent cavities to connect them. The sample inlet includes an inlet port and an air inlet tube connected to the inlet port. The inlet port is located on the bag body and communicates with one of the chambers. The air inlet tube is used to connect to the outside atmosphere to ensure that the air pressure in each chamber is consistent with the outside atmospheric pressure. A sampling section is provided on the bag body. There are multiple sampling sections, and one sampling section corresponds to one cavity.
2. The cell cryopreservation bag as described in claim 1, characterized in that, The cell cryopreservation bag has a first state and a second state. When the cell cryopreservation bag is in the first state, the channel connects to adjacent cavities. When the cell cryopreservation bag is in the second state, the channel is closed and adjacent cavities are not connected.
3. The cell cryopreservation bag as described in claim 2, characterized in that, A strip-shaped slit is provided between adjacent cavities to separate them, and the channel is located at both ends of the strip-shaped slit.
4. The cell cryopreservation bag as described in claim 3, characterized in that, The cell cryopreservation bag also has a third state, in which adjacent cavities are separated along the strip seam, and each cavity remains independent.
5. The cell cryopreservation bag as described in claim 1, characterized in that, The air inlet tube is equipped with a filter, and the filter is equipped with a Luer screw cap.
6. The cell cryopreservation bag as described in claim 5, characterized in that, The air inlet tube is also equipped with a clamp valve, which is located between the filter and the inlet.
7. The cell cryopreservation bag as described in claim 1, characterized in that, Each of the sampling units includes a sampling port and a soft cap that seals the sampling port, the sampling port being in communication with the cavity, and the soft cap being configured to be removable to expose the sampling port.
8. The cell cryopreservation bag as described in claim 1, characterized in that, The sample inlet also includes a filling conduit connected to the sample inlet, and the filling conduit satisfies at least one of the following conditions: The filling tubing is provided with a rubber stopper, which is configured to be punctured by the needle of a syringe to inject the sample from the syringe into the inlet and into the cavity; The filling tubing is provided with a Luer connector, which is configured to connect to a syringe to inject a sample from the syringe into the inlet and into the cavity.
9. The cell cryopreservation bag as described in claim 8, characterized in that, The Luer connector is a male Luer connector, and the filling conduit is equipped with a clamp valve on the side near the male Luer connector.
10. The cell cryopreservation bag as described in claim 8, characterized in that, The Luer connector is a female Luer connector, and the filling conduit is equipped with a clamp valve on the side near the female Luer connector.