Nucleic acid extraction instrument

Abstract

The nucleic acid extraction instrument comprises a base, a magnetic rod assembly, a magnetic rod sleeve assembly and a driving mechanism, a test tube seat is arranged on the base, a test tube is placed in the test tube seat, the magnetic rod assembly and the magnetic rod sleeve assembly are arranged above the test tube, and the driving mechanism respectively drives the magnetic rod assembly and the magnetic rod sleeve assembly to move up and down and left and right. The device has the advantages that the magnetic bar driving mechanism and the magnetic bar sleeve driving mechanism are ingeniously designed together, so that the whole device is compact, small and exquisite, can complete full-automatic nucleic acid extraction, and is suitable for being carried out for use.

Description

TECHNICAL FIELD

[0001] The utility model relates to medical instrument technical field, especially a kind of nucleic acid extraction instrument. BACKGROUND

[0002] Magnetic bead method nucleic acid extraction is a new type of nucleic acid extraction technology with nano biomagnetic beads as carrier, nucleic acid molecules can be specifically recognized and combined with the silicon hydroxyl on the surface of magnetic beads, and aggregation or dispersion occurs under the action of external magnetic field, so as to carry out nucleic acid separation and purification. At present, for the extraction of DNA, the magnetic bead method nucleic acid extraction generally includes four main steps of lysis, binding, washing and elution, and each step can be realized by a variety of different methods alone or in combination.

[0003] The magnetic rod type nucleic acid extraction instrument adopts the magnetic separation method of magnetic rod method, transfers the magnetic beads and samples through the specially made magnetic rod sleeve, the magnetic beads are oriented and aggregated on the special magnetic rod sleeve on the surface of the magnetic rod, and the magnetic beads are transferred from the sample lysis solution to the washing solution and then to the elution solution through precise movement, so as to complete the automatic extraction process of nucleic acid measurement. The specific operation process is as follows:

[0004] Step 1, sample addition: tear the sealing film, and add liquid sample to the lysis liquid hole;

[0005] Step 2, lysis / binding: the lysis liquid lyses the sample (reacts for a period of time, and the bottom is heated to accelerate lysis during the period), and the magnetic rod sleeve (only the magnetic rod sleeve, without the magnetic rod) is inserted below the liquid surface to oscillate up and down to mix, so that the nucleic acid is suspended in the lysis liquid; after lysis is completed, the magnetic rod sleeve with the magnetic rod is inserted into the magnetic bead hole to suck up the magnetic beads and transfer them to the lysis liquid hole, the magnetic rod is taken out, and the magnetic rod sleeve is oscillated up and down to mix, so that the magnetic beads are uniformly dispersed in the lysis liquid to adsorb the nucleic acid, and after a period of time, the magnetic rod is inserted and then left to stand or oscillated up and down until the magnetic beads are all adsorbed on the magnetic rod sleeve;

[0006] Step 3, salt washing: the magnetic beads adsorbed from the lysis liquid are transferred to the salt washing hole, the magnetic rod is taken out, the magnetic rod sleeve is oscillated up and down to mix, so that the magnetic beads are uniformly dispersed in the salt washing liquid, and after a period of time, the magnetic rod is inserted and then left to stand or oscillated up and down until the magnetic beads are all adsorbed on the magnetic rod sleeve;

[0007] Step 4, washing: the magnetic beads after salt washing are taken out and transferred to the washing liquid, the magnetic rod sleeve is oscillated up and down to mix, so that the magnetic beads are uniformly dispersed in the washing liquid, and after a period of time, the magnetic rod is inserted and then left to stand or oscillated up and down until the magnetic beads are all adsorbed on the magnetic rod sleeve;

[0008] Step 5, elution: the washed magnetic beads are taken out, the magnetic rod is taken out, the magnetic rod sleeve is oscillated up and down to mix evenly so that the magnetic beads are uniformly dispersed in the eluent, generally heated to 65℃ / 5min, and the nucleic acid is separated from the magnetic beads. At a higher temperature, the magnetic rod is inserted and then left to stand or oscillated up and down until the magnetic beads are all adsorbed on the magnetic rod sleeve, the magnetic beads are recovered, and thus the work of the nucleic acid extractor is completed. Then, the reaction plate is taken out from the machine, and the eluent containing the nucleic acid is transferred manually with a pipette.

[0009] However, to complete the above operations, the existing automatic nucleic acid extractor has a complex mechanism and a large device size, which is not convenient to carry. SUMMARY

[0010] In order to solve the problems existing in the existing nucleic acid extractor, a new nucleic acid extractor is designed.

[0011] A nucleic acid extractor includes a base, a magnetic rod assembly, a magnetic rod sleeve assembly, and a driving mechanism. The base is provided with a test tube seat, the test tube is placed in the test tube seat, the magnetic rod assembly and the magnetic rod sleeve assembly are arranged above the test tube, and the driving mechanism drives the magnetic rod assembly and the magnetic rod sleeve assembly to move up and down and left and right respectively.

[0012] Preferably, the driving mechanism includes a left-right movement mechanism, a magnetic rod driving mechanism, and a magnetic rod sleeve driving mechanism. The left-right movement mechanism is installed on the base, the magnetic rod driving mechanism and the magnetic rod sleeve driving mechanism are installed on the left-right movement mechanism, the magnetic rod assembly is installed on the magnetic rod driving mechanism, and the magnetic rod sleeve assembly is installed on the magnetic rod sleeve driving mechanism.

[0013] Preferably, the left-right movement mechanism includes a guide rail, a sliding plate, a first motor, a first synchronous belt transmission assembly, and a vertical plate. The guide rail is installed on the base, the sliding plate is movably assembled on the guide rail, the first motor is installed on the base, the first synchronous belt transmission assembly is installed on the base, the driving shaft of the first motor is connected with the driving shaft of the first motor, the synchronous belt of the first synchronous belt transmission assembly is connected with the sliding plate, and the vertical plate is installed on the sliding plate.

[0014] Preferably, the magnetic rod driving mechanism includes a second motor, a second synchronous belt transmission assembly, and a magnetic rod support. The second motor and the second synchronous belt transmission assembly are respectively installed on the vertical plate, the driving shaft of the second motor is connected with the driving shaft of the second motor, the magnetic rod support is fixed on the synchronous belt of the second synchronous belt transmission assembly, and the magnetic rod assembly is installed on the magnetic rod support.

[0015] Preferably, the magnetic rod sleeve driving mechanism comprises a third motor, a third synchronous belt transmission assembly and a magnetic rod sleeve support, the third motor and the third synchronous belt transmission assembly are respectively installed on the vertical plate, the driving wheel of the third synchronous belt transmission assembly is connected with the power output shaft of the third motor, the magnetic rod sleeve support is fixed on the synchronous belt of the third synchronous belt transmission assembly, and the magnetic rod sleeve assembly is installed on the magnetic rod sleeve support.

[0016] Preferably, vertical guide rails are arranged on the sliding plate, a first sliding groove is arranged on the magnetic rod support, and a second sliding groove is arranged on the magnetic rod sleeve support, and the first sliding groove and the second sliding groove are both assembled on the vertical guide rails.

[0017] Preferably, the magnetic rod assembly comprises a fixing frame and a plurality of magnetic rods.

[0018] Preferably, the magnetic rod sleeve assembly comprises a magnetic rod sleeve frame, a magnetic rod sleeve fixing plate and an elastic sheet, the bottom of the magnetic rod sleeve frame is provided with a fixing groove, the magnetic rod sleeve frame is uniformly provided with magnetic rod holes, the magnetic rod sleeve fixing plate is provided with a magnetic rod sleeve, the magnetic rod sleeve fixing plate is inserted into the fixing groove, and the elastic sheet is fixed on the magnetic rod sleeve frame and locks the magnetic rod sleeve fixing plate on the magnetic rod sleeve frame.

[0019] Preferably, baffles are arranged on the sliding plate.

[0020] Preferably, the utility model also comprises a protective cover, the protective cover is installed above the test tube seat, the base is provided with a shell, the shell is provided with a turnover door, and the turnover door is located at the test tube seat.

[0021] The utility model discloses the advantages of the device: the magnetic rod driving mechanism and the magnetic rod sleeve driving mechanism are designed together, so that the whole device is compact and small and can complete the automatic nucleic acid extraction, is suitable for carrying and going out and uses. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 It is the internal structure schematic diagram of the utility model Figure 1 ;

[0023] Figure 2 It is the internal structure schematic diagram of the utility model Figure 2 ;

[0024] Figure 3 It is the internal structure schematic diagram of the utility model Figure 3 ;

[0025] Figure 4 It is the internal structure schematic diagram of the utility model Figure 4 ;

[0026] Figure 5 It is the structure schematic diagram of the magnetic rod assembly and the magnetic rod sleeve assembly of the utility model;

[0027] Figure 6It is the magnetic rod sleeve assembly structure schematic diagram of the utility model;

[0028] Figure 7 It is the whole structure schematic diagram of the utility model;

[0029] Figure 8 It is the internal protective cover structure schematic diagram of the utility model.

[0030] In the drawing: 10, base; 11, test tube seat; 12, protective cover; 13, shell; 14, turnover door; 20, magnetic rod assembly; 201, fixed frame; 202 magnetic rod; 30, magnetic rod sleeve assembly; 301, magnetic rod sleeve frame; 302, magnetic rod sleeve fixed plate; 303, elastic sheet; 304, fixed groove; 305, magnetic rod hole; 306, magnetic rod sleeve; 40, drive mechanism; 41, left and right movement mechanism; 411, guide rail; 412, sliding plate; 413, first motor; 414, first synchronous belt transmission assembly; 415, vertical plate; 416, position sensing sensor; 42, magnetic rod drive mechanism; 421, second motor; 422, second synchronous belt transmission assembly; 423, magnetic rod support; 424, first sliding groove; 43, magnetic rod sleeve drive mechanism; 431, third motor; 432, third synchronous belt transmission assembly; 433, magnetic rod sleeve support; 434, second sliding groove; 44, vertical guide rail; 45, baffle. DETAILED DESCRIPTION

[0031] The principles and characteristics of the utility model are described below in combination with the drawings, and the examples are only used to explain the utility model and not used to limit the scope of the utility model.

[0032] As Figure 1 Shown, a nucleic acid extraction instrument, including base 10, magnetic rod assembly 20, magnetic rod sleeve assembly 30 and drive mechanism 40, the base 10 is equipped with test tube seat 11, and test tube is placed in test tube seat 11, and magnetic rod assembly 20 and magnetic rod sleeve assembly 30 are arranged above test tube, and drive mechanism 40 drives magnetic rod assembly 20 and magnetic rod sleeve assembly 30 to move up and down and left and right respectively, and magnetic rod assembly 20 and magnetic rod sleeve assembly 30 operate nucleic acid and magnetic beads in test tube.

[0033] Specifically, drive mechanism 40 includes left and right movement mechanism 41, magnetic rod drive mechanism 42 and magnetic rod sleeve drive mechanism 43, left and right movement mechanism 41 is installed on base 10, magnetic rod drive mechanism 42 and magnetic rod sleeve drive mechanism 43 are installed on left and right movement mechanism 41, the magnetic rod assembly 20 is installed on magnetic rod drive mechanism 42, the magnetic rod sleeve assembly 30 is installed on magnetic rod sleeve drive mechanism 43, left and right movement mechanism 41 can drive magnetic rod drive mechanism 42 and magnetic rod sleeve drive mechanism 43 to move left and right, magnetic rod drive mechanism 42 drives magnetic rod assembly 20 to move up and down, and magnetic rod sleeve drive mechanism 43 drives magnetic rod sleeve assembly 30 to move up and down;

[0034] Specifically, the left and right movement mechanism 41 includes a guide rail 411, a sliding plate 412, a first motor 413, a first synchronous belt transmission assembly 414 and a vertical plate 415, the guide rail 411 is mounted on the base 10, the sliding plate 412 is movably assembled on the guide rail 411, the first motor 413 is mounted on the base 10, the first synchronous belt transmission assembly 414 is mounted on the base 10, and the driving wheel of the first synchronous belt transmission assembly 414 is connected with the power output shaft of the first motor 413, the synchronous belt of the first synchronous belt transmission assembly 414 is connected with the sliding plate 412, the vertical plate 415 is mounted on the sliding plate 412, the synchronous belt transmission assembly includes a synchronous belt and two synchronous wheels, which are common prior art, so they are not described in detail, the first motor 413 can drive the first synchronous belt transmission assembly 414 to rotate, and the sliding plate 412 is fixedly connected with the synchronous belt, so the sliding plate 412 can move with the synchronous belt, and a position sensing sensor 416 for limiting is arranged at the bottom of the synchronous belt transmission assembly, so that the sliding plate 412 can only slide in the fixed position of the guide rail 411.

[0035] Specifically, the magnetic rod driving mechanism 42 includes a second motor 421, a second synchronous belt transmission assembly 422 and a magnetic rod support 423, the second motor 421 and the second synchronous belt transmission assembly 422 are respectively mounted on the vertical plate 415, and the driving wheel of the second synchronous belt transmission assembly 422 is connected with the power output shaft of the second motor 421, the magnetic rod support 423 is fixed on the synchronous belt of the second synchronous belt transmission assembly 422, and the magnetic rod assembly 20 is mounted on the magnetic rod support 423, the second motor 421 drives the second synchronous belt transmission assembly 422 to move, so that the magnetic rod support 423 can move up and down.

[0036] Specifically, the magnetic rod sleeve driving mechanism 43 includes a third motor 431, a third synchronous belt transmission assembly 432 and a magnetic rod sleeve support 433, the third motor 431 and the third synchronous belt transmission assembly 432 are respectively mounted on the vertical plate 415, and the driving wheel of the third synchronous belt transmission assembly 432 is connected with the power output shaft of the third motor 431, the magnetic rod sleeve support 433 is fixed on the synchronous belt of the third synchronous belt transmission assembly 432, and the magnetic rod sleeve assembly 30 is mounted on the magnetic rod sleeve support 433, the third motor 431 drives the third synchronous belt transmission assembly 432 to move, so that the magnetic rod sleeve support 433 can move up and down.

[0037] The vertical guide rail 44 is arranged on the sliding plate 412, the first sliding groove 424 is arranged on the magnetic rod support 423, the second sliding groove 434 is arranged on the magnetic rod sleeve support 433, the first sliding groove 424 and the second sliding groove 434 are assembled on the vertical guide rail 44, the magnetic rod support 423 and the magnetic rod sleeve support 433 can only move up and down and cannot deviate by limiting the vertical guide rail 44, the two are in the same track, the magnetic rod support 423 is above the magnetic rod sleeve support 433, and the two occupy the same space, so that the volume of the whole device is reduced.

[0038] The magnetic rod assembly 20 is composed of a fixing frame 201 and a group of magnetic rods 202 arranged uniformly; the magnetic rod sleeve assembly 30 comprises a magnetic rod sleeve frame 301, a magnetic rod sleeve fixing plate 302 and an elastic sheet 303, the bottom of the magnetic rod sleeve frame 301 is provided with a fixing groove 304, the magnetic rod sleeve frame 301 is uniformly provided with magnetic rod holes 305, the magnetic rod sleeve fixing plate 302 is provided with a magnetic rod sleeve 306, the magnetic rod sleeve fixing plate 302 is inserted on the fixing groove 304, the elastic sheet 303 is fixed on the magnetic rod sleeve frame 301 and locks the magnetic rod sleeve fixing plate 302 on the magnetic rod sleeve frame 301, and the magnetic rod sleeve 306 can be conveniently taken off from the magnetic rod sleeve fixing plate 302 through the above arrangement.

[0039] In order to avoid the operation of the driving mechanism from causing pollution to the nucleic acid operation, the baffle 45 is arranged on the sliding plate 412, the baffle moves along with the sliding plate 412, the baffle 45 has an opening through which the magnetic rod support 423 and the magnetic rod sleeve support 433 pass and smoothly move up and down, and the baffle 45 plays a role of isolating the nucleic acid extraction operation area and the driving mechanism.

[0040] The protective cover 12 is arranged on the test tube seat 11, and the space formed between the protective cover and the test tube seat 11 is the nucleic acid extraction operation area; the shell 13 is arranged on the base 10, the turnover door 14 is arranged on the shell 13, and the turnover door 14 is located at the test tube seat 11, so that the test tube to be extracted can be put into the test tube seat 11 by opening the turnover door, and the nucleic acid extraction operation can be performed.

[0041] The specific nucleic acid extraction operation steps are as follows:

[0042] Step 1, sample adding: obtain a 48-hole plate test tube, manually add a lysis solution, a combination solution, a washing solution and an elution solution into the hole positions of the 48-hole plate in sequence, usually one row of eight hole positions is one area, for example, the first row of eight holes contains the lysis solution, the second row of eight holes contains the combination solution, the third row of eight holes contains the first washing solution, the fourth row of eight holes contains the second washing solution, the fifth row of eight holes contains the elution solution, and the sixth row of holes contains the magnetic beads; the position containing the lysis solution corresponds to a heating module; then the sample is added into the lysis solution;

[0043] Step 2, lysis / coupling: the lysis solution lyses the sample (reacts for a period of time, during which the bottom is heated to accelerate lysis), the magnetic rod sleeve driving mechanism 43 drives the magnetic rod sleeve 306 to move downward, the magnetic rod sleeve is inserted below the liquid surface to mix uniformly by oscillating up and down, so that the nucleic acid is suspended in the lysis solution; after lysis is completed, the magnetic rod sleeve 306 is withdrawn upward, the left and right movement mechanism 41 drives the magnetic rod 202 and the magnetic rod sleeve 306 as a whole to move above the sixth row of magnetic bead holes, then drives the magnetic rod sleeve with the magnetic rod to extend into the magnetic bead hole to suck up the magnetic beads, then rises to transfer to the lysis solution hole, the magnetic rod is taken out, and the magnetic rod sleeve is oscillated up and down to mix uniformly so that the magnetic beads are uniformly dispersed in the lysis solution to adsorb the nucleic acid, after a period of time, the magnetic rod is inserted and then left to stand or oscillated up and down until the magnetic beads are all adsorbed on the magnetic rod sleeve;

[0044] Step 3, salt washing: the magnetic beads adsorbed from the lysis solution are transferred to the salt washing hole, the magnetic rod is taken out, the magnetic rod sleeve is oscillated up and down to mix uniformly so that the magnetic beads are uniformly dispersed in the salt washing solution, after a period of time, the magnetic rod is inserted and then left to stand or oscillated up and down until the magnetic beads are all adsorbed on the magnetic rod sleeve;

[0045] Step 4, washing: the magnetic beads after salt washing are taken out and transferred to the washing solution, the magnetic rod sleeve is oscillated up and down to mix uniformly so that the magnetic beads are uniformly dispersed in the washing solution, after a period of time, the magnetic rod is inserted and then left to stand or oscillated up and down until the magnetic beads are all adsorbed on the magnetic rod sleeve;

[0046] Step 5, elution: the washed magnetic beads are taken out, the magnetic rod is taken out, the magnetic rod sleeve is oscillated up and down to mix uniformly so that the magnetic beads are uniformly dispersed in the elution solution, generally heated to 65℃ / 5min, left to stand, heated to make the nucleic acid separate from the magnetic beads, at a higher temperature, the magnetic rod is inserted and then left to stand or oscillated up and down until the magnetic beads are all adsorbed on the magnetic rod sleeve, and the magnetic beads are recovered; after the magnetic beads are recovered, the instrument operation is completed.

[0047] The above only describes preferred embodiments of the present application and is not intended to limit the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A nucleic acid extraction instrument, characterized in that, The device includes a base (10), a magnetic rod assembly (20), a magnetic rod sleeve assembly (30), and a drive mechanism (40). The base (10) is provided with a test tube holder (11), in which a test tube is placed. The magnetic rod assembly (20) and the magnetic rod sleeve assembly (30) are positioned above the test tube. The drive mechanism (40) drives the magnetic rod assembly (20) and the magnetic rod sleeve assembly (30) to move up, down, left, and right, respectively.

2. The nucleic acid extraction instrument as described in claim 1, characterized in that, The drive mechanism (40) includes a left and right motion mechanism (41), a magnetic rod drive mechanism (42), and a magnetic rod sleeve drive mechanism (43). The left and right motion mechanism (41) is mounted on the base, the magnetic rod drive mechanism (42) and the magnetic rod sleeve drive mechanism (43) are mounted on the left and right motion mechanism (41), the magnetic rod assembly (20) is mounted on the magnetic rod drive mechanism (42), and the magnetic rod sleeve assembly (30) is mounted on the magnetic rod sleeve drive mechanism (43).

3. The nucleic acid extraction instrument as described in claim 2, characterized in that, The left and right movement mechanism (41) includes a guide rail (411), a slide plate (412), a first motor (413), a first synchronous belt drive assembly (414), and a vertical plate (415). The guide rail (411) is mounted on the base (10), the slide plate (412) is movably mounted on the guide rail (411), the first motor (413) is mounted on the base (10), the first synchronous belt drive assembly (414) is mounted on the base (10), and the drive wheel of the first synchronous belt drive assembly (414) is connected to the power output shaft of the first motor (413). The synchronous belt of the first synchronous belt drive assembly (414) is connected to the slide plate (412), and the vertical plate (415) is mounted on the slide plate (412).

4. The nucleic acid extraction instrument as described in claim 3, characterized in that, The magnetic rod drive mechanism (42) includes a second motor (421), a second synchronous belt drive assembly (422), and a magnetic rod bracket (423). The second motor (421) and the second synchronous belt drive assembly (422) are respectively mounted on the vertical plate (415), and the drive wheel of the second synchronous belt drive assembly (422) is connected to the power output shaft of the second motor (421). The magnetic rod bracket (423) is fixed on the synchronous belt of the second synchronous belt drive assembly (422), and the magnetic rod assembly (20) is mounted on the magnetic rod bracket (423).

5. The nucleic acid extraction instrument as described in claim 4, characterized in that, The magnetic rod sleeve drive mechanism (43) includes a third motor (431), a third synchronous belt drive assembly (432), and a magnetic rod sleeve bracket (433). The third motor (431) and the third synchronous belt drive assembly (432) are respectively mounted on the vertical plate (415), and the drive wheel of the third synchronous belt drive assembly (432) is connected to the power output shaft of the third motor (431). The magnetic rod sleeve bracket (433) is fixed on the synchronous belt of the third synchronous belt drive assembly (432), and the magnetic rod sleeve assembly (30) is mounted on the magnetic rod sleeve bracket (433).

6. The nucleic acid extraction instrument as described in claim 5, characterized in that, The slide plate (412) is provided with a vertical guide rail (44), the magnetic rod support (423) is provided with a first slide groove (424), and the magnetic rod sleeve support (433) is provided with a second slide groove (434). The first slide groove (424) and the second slide groove (434) are both mounted on the vertical guide rail (44).

7. The nucleic acid extraction instrument as described in claim 1, characterized in that, The magnetic rod assembly (20) includes a fixture (201) and a set of magnetic rods (202).

8. The nucleic acid extraction instrument as described in claim 1, characterized in that, The magnetic rod sleeve assembly (30) includes a magnetic rod sleeve frame (301), a magnetic rod sleeve fixing plate (302), and a spring piece (303). The bottom of the magnetic rod sleeve frame (301) is provided with a fixing groove (304), and magnetic rod holes (305) are evenly arranged on the magnetic rod sleeve frame (301). A magnetic rod sleeve (306) is provided on the magnetic rod sleeve fixing plate (302). The magnetic rod sleeve fixing plate (302) is inserted into the fixing groove (304), and the spring piece (303) is fixed on the magnetic rod sleeve frame (301) and locks the magnetic rod sleeve fixing plate (302) on the magnetic rod sleeve frame (301).

9. A nucleic acid extraction instrument as described in claim 1, characterized in that, A baffle (45) is provided on the skateboard (412).

10. A nucleic acid extraction instrument as described in claim 1, characterized in that, It also includes a protective cover (12), which is installed above the test tube holder (11). The base (10) is provided with a housing (13), which is provided with a flip door (14), and the flip door (14) is located at the test tube holder (11).

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