Micro-fluidic chip for blood coagulation detection

Abstract

The utility model discloses a microfluidic chip for blood coagulation detection, which comprises a disc used for being mounted on blood coagulation detection equipment so as to enable the disc to rotate at a high speed on the blood coagulation detection equipment; the flow channel unit is arranged on the disc, the flow channel unit comprises a sample adding space, a reagent bin and a colorimetric bin, the sample adding space is provided with an opening, the reagent bin is used for containing a freeze-drying reagent, the sample adding space is communicated with the reagent bin, the reagent bin is communicated with the colorimetric bin, and the colorimetric bin is used for containing the freeze-drying reagent. A first distance is formed between the sample adding space and the center of the disc in the radial direction, a second distance is formed between the reagent bin and the center of the disc in the radial direction, a third distance is formed between the colorimetric bin and the center of the disc in the radial direction, the first distance is smaller than the second distance, and the second distance is smaller than the third distance; the blood coagulation detection device can be beneficial to improving the blood coagulation detection efficiency and convenience.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, and in particular to a microfluidic sheet for coagulation detection. Background Technology

[0002] With the continuous development of medical technology, coagulation testing plays an increasingly important role in clinical diagnosis and treatment. However, traditional coagulation testing methods are often cumbersome, time-consuming, and require specialized equipment and personnel, limiting their widespread application in emergency medicine and primary healthcare institutions. Therefore, developing a rapid, simple, and portable coagulation testing tool is particularly important. Microfluidic technology, with its unique advantages such as speed, low overall cost, and low sample consumption, provides a new solution for coagulation testing. Utility Model Content

[0003] This invention provides a microfluidic sheet for coagulation detection, which can help improve the efficiency and convenience of coagulation detection.

[0004] To address the aforementioned technical problems, this invention provides a microfluidic sheet for coagulation detection, comprising:

[0005] A disc for mounting on a coagulation testing device to enable the disc to rotate at high speed on the coagulation testing device;

[0006] A flow channel unit is disposed on the disk. The flow channel unit includes a sample dispensing space, a reagent chamber, and a colorimetric chamber. The sample dispensing space has an opening. The reagent chamber is used to contain lyophilized reagents. The sample dispensing space is connected to the reagent chamber. The reagent chamber is connected to the colorimetric chamber. The sample dispensing space is radially at a first distance from the center of the disk. The reagent chamber is radially at a second distance from the center of the disk. The colorimetric chamber is radially at a third distance from the center of the disk. The first distance is less than the second distance, and the second distance is less than the third distance.

[0007] As a preferred embodiment of the above technical solution, the colorimetric chamber is provided with stirring magnetic beads.

[0008] As a preferred embodiment of the above technical solution, a colorimetric protrusion is provided at the bottom center of the colorimetric chamber.

[0009] As a preferred embodiment of the above technical solution, the reagent compartment includes a first reagent compartment and a second reagent compartment, which are used to contain different lyophilized reagents. The flow channel unit further includes a flow channel switching structure, which is located between the sample dispensing space and the reagent compartment. The flow channel switching structure is used to switch the flow path between the sample dispensing space and the first and second reagent compartments.

[0010] As a preferred embodiment of the above technical solution, the flow channel switching structure includes a switching chamber and a valve ball. The sample dispensing space and the switching chamber are connected through a connecting channel. The switching chamber is connected to the first reagent chamber and the second reagent chamber through a first connecting hole and a second connecting hole, respectively. A protrusion is formed at the bottom and middle position of the switching chamber. The first connecting hole and the second connecting hole are respectively located on both sides of the protrusion. The valve ball is located in the switching chamber, and the size of the valve ball corresponds to the size of the connecting hole.

[0011] As a preferred embodiment of the above technical solution, the flow channel unit further includes a first air channel and a second air channel. The first air channel is connected to the first reagent chamber, and the second air channel is connected to the second reagent chamber. The first air channel is close to the top of the first reagent chamber, and the second air channel is close to the top of the second reagent chamber. The disk is provided with a first air hole and a second air hole corresponding to the first air channel and the second air channel.

[0012] As a preferred embodiment of the above technical solution, the flow channel unit further includes a mixing channel, a first inclined channel, and a second inclined channel. The first reagent chamber is connected to the mixing channel through the first inclined channel, the second reagent chamber is connected to the mixing channel through the second inclined channel, and the mixing channel is connected to the colorimetric chamber.

[0013] As a preferred embodiment of the above technical solution, the mixing channel is provided with multiple vertical plates, which are arranged in an alternating pattern.

[0014] As a preferred embodiment of the above technical solution, the connection between the first inclined channel and the first reagent chamber is higher than the bottom of the first reagent chamber to form a step at the connection between the first inclined channel and the first reagent chamber, and the connection between the second inclined channel and the second reagent chamber is higher than the bottom of the second reagent chamber to form a step at the connection between the second inclined channel and the second reagent chamber.

[0015] As a preferred embodiment of the above technical solution, the flow channel unit further includes a first buffer chamber and a second buffer chamber. The first buffer chamber is connected to the colorimetric chamber through a first buffer air channel, and the second buffer chamber is connected to the colorimetric chamber through a second buffer air channel. The disc is provided with a first buffer air hole and a second buffer air hole corresponding to the first buffer air channel and the second buffer air channel. The first buffer air channel and the second buffer air channel are respectively located on both sides of the colorimetric protrusion.

[0016] This invention provides a microfluidic plate for coagulation detection, comprising a disc and a flow channel unit. During operation, the disc is installed on the coagulation detection device, and the collected blood sample is added to the sample application space. Since the sample application space, reagent chamber, and colorimetric chamber are sequentially located at different distances from the disc, the high-speed rotation of the disc causes the collected blood sample to be drawn into the reagent chamber under centrifugal force. Because the reagent chamber contains pre-stored lyophilized reagents, the collected blood sample fuses with the lyophilized reagents inside the chamber. Under further centrifugal force, the blood sample and reagents enter the colorimetric chamber together. The detection device performs colorimetric detection on the blood sample in the colorimetric chamber, which improves the efficiency and convenience of coagulation detection.

[0017] The above description is merely an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, and to make the above and other objects, features and advantages of this utility model more obvious and understandable, specific embodiments of this utility model are given below. Attached Figure Description

[0018] Figure 1 A three-dimensional structural schematic diagram of a microfluidic sheet for coagulation detection is shown in an embodiment of the present invention;

[0019] Figure 2 A cross-sectional view of a microfluidic sheet for coagulation detection according to an embodiment of the present invention is shown;

[0020] Figure 3 A partial cross-sectional view of a microfluidic sheet for coagulation detection according to an embodiment of the present invention is shown;

[0021] Figure 4 A partial cross-sectional view of a microfluidic sheet for coagulation detection in an embodiment of the present invention is shown from another angle;

[0022] In the diagram: 10, disk; 20, flow channel unit; 101, first vent; 102, second vent; 103, first buffer vent; 104, second buffer vent; 201, sample loading space; 202, connecting channel; 203, switching chamber; 204, protrusion; 205, reagent chamber; 206, first air passage; 207, second air passage; 208, mixing channel; 209, colorimetric chamber; 210, colorimetric protrusion; 211, first buffer chamber; 212, second buffer chamber; 213, vertical plate; 214, first buffer air passage; 215, second buffer air passage; 216, first oblique channel; 217, second oblique channel; 218, first connecting hole; 219, second connecting hole; 2051, first reagent chamber; 2052, second reagent chamber. Detailed Implementation

[0023] To make the objectives, features, and advantages of this utility model more apparent and understandable, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0024] See Figures 1 to 4 This utility model provides a microfluidic sheet for coagulation detection, comprising:

[0025] Disk 10 is used to be mounted on a coagulation testing device so that disk 10 can rotate at high speed on the coagulation testing device;

[0026] A flow channel unit 20 is disposed on the disc 10. The flow channel unit 20 includes a sample dispensing space 201, a reagent chamber 205, and a colorimetric chamber 209. The sample dispensing space 201 has an opening. The reagent chamber 205 is used to contain lyophilized reagents. The sample dispensing space 201 is connected to the reagent chamber 205, and the reagent chamber 205 is connected to the colorimetric chamber 209. The sample dispensing space 201 and the center of the disc 10 are radially at a first distance. The reagent chamber 205 and the center of the disc 10 are radially at a second distance. The colorimetric chamber 209 and the center of the disc 10 are radially at a third distance. The first distance is smaller than the second distance, and the second distance is smaller than the third distance.

[0027] This embodiment provides a microfluidic plate for coagulation detection, comprising a disc 10 and a flow channel unit 20. During operation, the disc 10 is installed on a coagulation detection device, and the collected blood sample is added to the sample application space 201. Since the sample application space 201, reagent chamber 205, and colorimetric chamber 209 are located progressively further from the disc 10, the high-speed rotation of the disc 10 causes the collected blood sample to be drawn into the reagent chamber 205 under centrifugal force. Because the reagent chamber 205 contains pre-stored lyophilized reagents, the collected blood sample fuses with the lyophilized reagents inside the reagent chamber 205. Under further centrifugal force, the blood sample and reagents enter the colorimetric chamber 209 together. The detection device performs colorimetric detection on the blood sample in the colorimetric chamber 209, which improves the efficiency and convenience of coagulation detection.

[0028] In a further embodiment of this invention, a stirring magnetic bead (not shown in the figure) is provided in the colorimetric chamber 209.

[0029] In this embodiment, a stirring magnetic bead is provided in the colorimetric chamber 209. Under the magnetic force of the device, the blood sample in the colorimetric chamber 209 can be quickly stirred by the stirring magnetic bead to make the mixture more uniform.

[0030] In a further embodiment of this invention, a colorimetric protrusion 210 is provided at the bottom center of the colorimetric bin 209.

[0031] In this embodiment, a colorimetric protrusion 210 is provided at the bottom center of the colorimetric chamber 209, which allows the stirring magnetic bead to remain outside the colorimetric protrusion 210 during colorimetric detection, preventing the stirring magnetic bead from blocking the colorimetric light.

[0032] Specifically, in this embodiment, the cross-section of the colorimetric protrusion 210 is trapezoidal, with slopes on both sides, which makes it easier for the stirring magnetic beads to slide down the slopes to the bottom of the colorimetric protrusion 210, thereby ensuring that the stirring magnetic beads remain on the side of the colorimetric chamber 209.

[0033] In a further embodiment of this example, the reagent compartment 205 includes a first reagent compartment 2051 and a second reagent compartment 2052, which are respectively used to contain different lyophilized reagents. The flow channel unit 20 also includes a flow channel switching structure, which is located between the sample dispensing space 201 and the reagent compartment 205. The flow channel switching structure is used to switch the flow path between the sample dispensing space 201 and the first reagent compartment 2051 and the second reagent compartment 2052.

[0034] In this embodiment, different lyophilized reagents are set in the first reagent compartment 2051 and the second reagent compartment 2052. The flow channel switching structure switches the connection between the sample addition space 201 and the first reagent compartment 2051 and the second reagent compartment 2052 respectively, so as to realize the combination of different reagents with the sample. In the actual detection process, since two different reagents need to be added one after the other, the flow channel switching structure can be used to realize the order of addition of the two reagents.

[0035] In a further embodiment of this invention, the flow channel switching structure includes a switching chamber 203 and a valve ball (not shown in the figure). The sample dispensing space 201 and the switching chamber 203 are connected through a connecting channel 202. The switching chamber 203 is connected to the first reagent chamber 2051 and the second reagent chamber 2052 through a first connecting hole 218 and a second connecting hole 219, respectively. A protrusion 204 is formed at the bottom and middle position of the switching chamber 203. The first connecting hole 218 and the second connecting hole 219 are respectively located at the two protrusions of the switching chamber 203. The valve ball is located in the switching chamber 203, and the size of the valve ball corresponds to the size of the connecting hole.

[0036] In this embodiment, switching is achieved by rotating in both directions. Specifically, when rotating in the first direction, the valve ball blocks one connection port. When rotating in the second direction, the valve ball rolls to another connection port and blocks that connection port, thereby achieving switching.

[0037] In this embodiment, the protrusion 204 has ramps on both sides, which makes it easier for the valve ball to move in the switching chamber 203.

[0038] In a further embodiment of this invention, the flow channel unit 20 further includes a first air channel 206 and a second air channel 207. The first air channel 206 is connected to the first reagent chamber 2051, and the second air channel 207 is connected to the second reagent chamber 2052. The first air channel 206 is close to the top of the first reagent chamber 2051, and the second air channel 207 is close to the top of the second reagent chamber 2052. The disk 10 is provided with a first air hole 101 and a second air hole 102 corresponding to the first air channel 206 and the second air channel 207.

[0039] In this embodiment, the arrangement of the first air passage 206 and the second air passage 207, as well as the first air hole 101 and the second air hole 102, allows air to be discharged, facilitating sample flow.

[0040] In a further embodiment of this example, the flow channel unit 20 further includes a mixing channel 208, a first inclined channel 216, and a second inclined channel 217. The first reagent chamber 2051 is connected to the mixing channel 208 through the first inclined channel 216, and the second reagent chamber 2052 is connected to the mixing channel 208 through the second inclined channel 217. The mixing channel 208 is connected to the colorimetric chamber 209.

[0041] In this embodiment, the sample and reagent are first mixed as they flow through the mixing channel 208, and then further mixed by a stirring magnetic bead after entering the colorimetric chamber 209.

[0042] In a further embodiment of this invention, the mixing channel 208 is provided with a plurality of vertical plates 213, which are staggered.

[0043] The vertical plate 213 and its arrangement in this embodiment can better facilitate the mixing of samples and reagents.

[0044] In a further embodiment of this invention, the connection between the first inclined channel 216 and the first reagent compartment 2051 is higher than the bottom of the first reagent compartment 2051 to form a step at the connection between the first inclined channel 216 and the first reagent compartment 2051, and the connection between the second inclined channel 217 and the second reagent compartment 2052 is higher than the bottom of the second reagent compartment 2052 to form a step at the connection between the second inclined channel 217 and the second reagent compartment 2052.

[0045] In this embodiment, since a step is formed at the connection, the sample is located in the first reagent chamber 2051 and the second reagent chamber 2052 at the first rotation speed. This is beneficial for the thawing and dissolution of the lyophilized reagent and makes it easier to control the dissolution time with the reagent. After being fully thawed, the sample can cross the step and enter the next stage by accelerating to the second speed, which has a greater centrifugal force.

[0046] In a further embodiment of this invention, the flow channel unit 20 further includes a first buffer chamber 211 and a second buffer chamber 212. The first buffer chamber 211 is connected to the colorimetric chamber 209 through a first buffer air passage 214, and the second buffer chamber 212 is connected to the colorimetric chamber 209 through a second buffer air passage 215. The disc 10 is provided with a first buffer air hole 103 and a second buffer air hole 104 corresponding to the first buffer air passage 214 and the second buffer air passage 215. The first buffer air passage 214 and the second buffer air passage 215 are respectively located on both sides of the colorimetric protrusion 210.

[0047] In this embodiment, the first buffer chamber 211 and the second buffer chamber 212 can be used to receive samples that overflow from the colorimetric chamber 209.

[0048] In the description of this specification, references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. Furthermore, the described specific features, structures, materials, or characteristics may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of those different embodiments or examples.

[0049] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0050] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A microfluidic sheet for coagulation detection, characterized in that, include: A disc for mounting on a coagulation testing device to enable the disc to rotate at high speed on the coagulation testing device; A flow channel unit is disposed on the disk. The flow channel unit includes a sample dispensing space, a reagent chamber, and a colorimetric chamber. The sample dispensing space has an opening. The reagent chamber is used to contain lyophilized reagents. The sample dispensing space is connected to the reagent chamber. The reagent chamber is connected to the colorimetric chamber. The sample dispensing space is radially at a first distance from the center of the disk. The reagent chamber is radially at a second distance from the center of the disk. The colorimetric chamber is radially at a third distance from the center of the disk. The first distance is less than the second distance, and the second distance is less than the third distance.

2. The microfluidic sheet for coagulation detection according to claim 1, characterized in that, The colorimetric chamber is equipped with stirring magnetic beads.

3. The microfluidic sheet for coagulation detection according to claim 2, characterized in that, A colorimetric protrusion is provided at the bottom center of the colorimetric chamber.

4. The microfluidic sheet for coagulation detection according to claim 1, characterized in that, The reagent compartment includes a first reagent compartment and a second reagent compartment, which are used to contain different lyophilized reagents. The flow channel unit also includes a flow channel switching structure, which is located between the sample dispensing space and the reagent compartment. The flow channel switching structure is used to switch the flow path between the sample dispensing space and the first and second reagent compartments.

5. The microfluidic sheet for coagulation detection according to claim 4, characterized in that, The flow channel switching structure includes a switching chamber and a valve ball. The sample dispensing space is connected to the switching chamber through a connecting channel. The switching chamber is connected to the first reagent chamber and the second reagent chamber through a first connecting hole and a second connecting hole, respectively. A protrusion is formed at the bottom and middle of the switching chamber. The first connecting hole and the second connecting hole are respectively located on both sides of the protrusion. The valve ball is located in the switching chamber, and the size of the valve ball corresponds to the size of the connecting hole.

6. The microfluidic sheet for coagulation detection according to claim 5, characterized in that, The flow channel unit further includes a first air channel and a second air channel. The first air channel is connected to the first reagent chamber, and the second air channel is connected to the second reagent chamber. The first air channel is close to the top of the first reagent chamber, and the second air channel is close to the top of the second reagent chamber. The disk is provided with a first air hole and a second air hole corresponding to the first air channel and the second air channel.

7. The microfluidic sheet for coagulation detection according to claim 5, characterized in that, The flow channel unit further includes a mixing channel, a first inclined channel, and a second inclined channel. The first reagent chamber is connected to the mixing channel through the first inclined channel, and the second reagent chamber is connected to the mixing channel through the second inclined channel. The mixing channel is connected to the colorimetric chamber.

8. The microfluidic sheet for coagulation detection according to claim 7, characterized in that, The mixing channel is provided with multiple vertical plates, which are arranged in an alternating pattern.

9. The microfluidic sheet for coagulation detection according to claim 7, characterized in that, The opening connecting the first inclined channel and the first reagent compartment is higher than the bottom of the first reagent compartment to form a step at the connection between the first inclined channel and the first reagent compartment, and the opening connecting the second inclined channel and the second reagent compartment is higher than the bottom of the second reagent compartment to form a step at the connection between the second inclined channel and the second reagent compartment.

10. The microfluidic sheet for coagulation detection according to claim 3, characterized in that, The flow channel unit further includes a first buffer chamber and a second buffer chamber. The first buffer chamber is connected to the colorimetric chamber through a first buffer air channel, and the second buffer chamber is connected to the colorimetric chamber through a second buffer air channel. The disc is provided with a first buffer air hole and a second buffer air hole corresponding to the first buffer air channel and the second buffer air channel. The first buffer air channel and the second buffer air channel are respectively located on both sides of the colorimetric protrusion.

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