Sampling and homogenizing integrated appliance and sampling and homogenizing integrated appliance assembly
By integrating sampling and homogenization functions, the integrated sampling and homogenization device solves the problems of sample contamination and cumbersome operation, and achieves efficient and low-contamination sample processing.
Patent Information
- Application Number
- CN202520249961.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-17
- Publication Date
- 2026-01-27
- Estimated Expiration
- 2035-02-17
AI Technical Summary
In existing technologies, sample collection and homogenization are performed separately, which leads to a high risk of sample contamination and is cumbersome.
Design an integrated sampling and homogenization device that combines sampling and homogenization functions. The sample is obtained by inserting the needle into the tissue and directly enters the homogenization chamber. The sample is sealed with a sealing element to prevent sample transfer and is homogenized using homogenization beads.
It simplifies the operation process, reduces the risk of sample contamination, improves operational efficiency, and avoids sample contamination during multiple transfers.
Smart Images
Figure CN223837422U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of instruments for biological tissue homogenization experiments, specifically to an integrated sampling and homogenization instrument and an integrated sampling and homogenization instrument assembly. Background Technology
[0002] In species identification studies, it is necessary to extract DNA from the tissues to be identified. Tissue sample collection and homogenization are the main procedures for DNA extraction.
[0003] Traditional procedures involve separate sampling and homogenization processes. For example, tissue samples are taken from the tissue to be identified using surgical instruments, and then placed in centrifuge tubes or a separate homogenization device for homogenization. This approach, with separate sampling and homogenization steps requiring multiple operations, increases the possibility of sample contamination and cross-contamination. Utility Model Content
[0004] This invention provides an integrated sampling and homogenizing apparatus and an integrated sampling and homogenizing apparatus component to solve the technical problems of cumbersome sampling and homogenizing operations and easy sample contamination in the DNA extraction experiment of the prior art.
[0005] In a first aspect, the integrated sampling and homogenization apparatus provided by this utility model includes:
[0006] The tube body includes a main body and a needle body. The main body has a homogenization chamber with an opening for adding a homogenization medium into the homogenization chamber. The needle body has a lumen for inserting into the tissue to be sampled so that the tissue to be sampled enters the lumen. The lumen and the homogenization chamber are connected so that the tissue to be sampled entering the lumen can enter the homogenization chamber.
[0007] A first sealing element is used to seal the opening;
[0008] The second seal is used to seal the end of the needle portion that is away from the main body portion.
[0009] In one technical solution, the second sealing element includes a sealing cap and a sealing block. The sealing block is disposed at the bottom of the inner cavity of the sealing cap. The sealing cap is detachably and sealingly connected to the main body and covers the needle body. The sealing block is used to seal the end of the needle body.
[0010] In one technical solution, the sealing cap is threaded onto the main body.
[0011] In one technical solution, the lumen has an anti-backflow structure to prevent the sampled tissue that has entered the lumen from flowing back out of the lumen.
[0012] In one technical solution, the anti-backflow structure is a barb disposed on the inner wall of the tube, and the angle between the barb and the inner wall of the tube is between 15° and 35°.
[0013] In one technical solution, the main body includes a transition section for connecting with the needle body, the transition section being a tapered structure.
[0014] In one technical solution, the main body portion is provided with a tube opening at one end away from the needle body portion, the tube opening constitutes the opening, and the first sealing member is a sealing cap that can be detachably sealed to the opening, the sealing cap being connected to the main body portion by a flexible connecting piece.
[0015] In one technical solution, the diameter of the lumen is between 0.6mm and 1.6mm.
[0016] Secondly, this utility model provides an integrated sampling and homogenizing device assembly, including the integrated sampling and homogenizing device described in any of the above technical solutions, and further including homogenizing beads contained in the homogenizing chamber, the homogenizing beads being used to grind the tissue sample during homogenization.
[0017] In one technical solution, the homogenized beads include at least one of glass beads and metal beads.
[0018] The beneficial effects of this utility model are as follows:
[0019] This utility model discloses an integrated sampling and homogenizing device, which has a main body and a needle body on the tube. The tissue to be sampled can be punctured by the needle body and enter the lumen of the needle body as a tissue sample. Since the lumen of the needle body is connected to the homogenizing chamber of the main body, the tissue sample in the lumen can be squeezed into the homogenizing chamber by continuously puncturing the tissue to be sampled. After sampling, the needle body can be sealed by a second sealing element, and homogenizing medium can be added into the homogenizing chamber through the opening. Then, the opening is sealed by a first sealing element to complete the sealing of the entire integrated sampling and homogenizing device. Homogenization can then be performed directly through this integrated sampling and homogenizing device. The entire sampling and homogenizing process does not require changing the device, thus minimizing the risk of tissue sample contamination. Attached Figure Description
[0020] Figure 1 This is a schematic diagram of an embodiment of the integrated sampling and homogenizing apparatus component of this utility model, showing the homogenizing medium being contained.
[0021] Figure 2 This is a schematic diagram of the integrated sampling and homogenizing apparatus in one embodiment of the present invention.
[0022] List of feature names corresponding to the labels in the figure:
[0023] 1. Integrated sampling and homogenizing apparatus; 11. Main body; 111. Homogenizing chamber; 112. Transition section; 113. Inlet; 114. Flexible connecting piece; 12. Needle body; 121. Lumen; 122. Barb; 13. First sealing element; 14. Second sealing element; 141. Sealing cap; 142. Sealing block;
[0024] 2. Homogenized beads;
[0025] 3. Homogenizing medium. Detailed Implementation
[0026] The present application will now be described in further detail with reference to the accompanying drawings and specific embodiments. Similar elements in different embodiments are referred to by related similar element reference numerals. In the following embodiments, many details are described to facilitate a better understanding of the present application. However, those skilled in the art will readily recognize that some features may be omitted in different situations, or may be replaced by other elements, materials, or methods. In some cases, certain operations related to the present application are not shown or described in the specification. This is to avoid obscuring the core parts of the present application with excessive description. For those skilled in the art, detailed description of these related operations is not necessary; they can fully understand the related operations based on the description in the specification and general technical knowledge in the art.
[0027] Furthermore, the features, operations, or characteristics described in the specification can be combined in any suitable manner to form various embodiments, and the operational steps involved in each embodiment can also be rearranged or adjusted in a manner that is obvious to those skilled in the art. Therefore, the specification and drawings are only for clearly describing a particular embodiment and do not imply that they represent the necessary components and / or order.
[0028] The serial numbers assigned to components in this document, such as "first" and "second," are used only to distinguish the described objects and have no sequential or technical meaning. The terms "connection" and "linkage" used in this application, unless otherwise specified, include both direct and indirect connections (linkages).
[0029] In the process of sample collection and homogenization for species identification, to prevent sample contamination, this utility model integrates sampling and homogenization functions into a single sampling and homogenization device. Specifically, the tube includes a main body and a needle body. The main body has a homogenization chamber with an opening, and the needle body has a lumen that can pierce the tissue to be sampled. Thus, during sampling, tissue samples can be obtained by piercing the tissue to be sampled through the needle body. Since the lumen is connected to the homogenization chamber, the tissue sample in the lumen can enter the homogenization chamber. After sampling, the end of the needle body away from the main body is sealed by a second sealing element. Homogenization medium can be added to the homogenization chamber through the opening, and then the opening is sealed by the first sealing element. Afterward, homogenization can be performed directly through this integrated sampling and homogenization device. The entire process does not require changing the device, simplifying the operation and minimizing the risk of tissue sample contamination. It can also be used once and for all, avoiding the hassle of cleaning and maintenance.
[0030] One embodiment of the integrated sampling and homogenizing apparatus component of this utility model is provided below. Figure 1 The integrated sampling and homogenizing device assembly includes an integrated sampling and homogenizing device 1 and a homogenizing bead 2.
[0031] Please refer to Figure 2 The integrated sampling and homogenizing device 1 includes a tube body, which in turn includes a main body 11 and a needle body 12. The integrated sampling and homogenizing device 1 also includes a first sealing element 13 and a second sealing element 14 for sealing and cooperating with the main body 11 and the needle body 12, respectively.
[0032] The main body 11 is a tubular structure with a circular cross-section, and its inner cavity is a homogenization chamber 111 for holding the homogenization medium 3. The homogenization medium is a solution that can maintain a certain physiological state of tissue cells and facilitate subsequent analysis. In actual operation, the selection of homogenization medium will vary depending on factors such as experimental purpose, tissue type, and experimental conditions. The specific selection, composition, and usage of the homogenization medium are existing technologies and will not be elaborated here.
[0033] The homogenization chamber 111 has an opening through which homogenizing medium can be added. After homogenization is completed, the tissue homogenate can be taken out from the homogenization chamber 111 through the opening for subsequent experiments. The material of the main body 11 can be sanitary PVC (polyvinyl chloride), PP (polypropylene), or glass, stainless steel, etc.
[0034] The needle body 12 has a smaller diameter and a lumen 121, which is connected to the homogenization chamber 111. The needle body 12 has higher hardness than the main body 11, allowing it to penetrate the sampled tissue. By inserting the needle body 12 into the sampled tissue, the sampled tissue can enter the lumen 121 of the needle body 12. The sampled tissue entering the lumen 121 becomes a tissue sample. By repeatedly piercing the sampled tissue with the needle body 12, the tissue sample can be inserted into the homogenization chamber 111.
[0035] Regarding the connection method between the main body 11 and the needle body 12, in one embodiment, the main body 11 is made of plastic and the needle body 12 is made of metal. The needle body 12 is formed first, and then the main body 11 is injection molded onto the needle body 12. In other embodiments, the main body 11 and the needle body 12 can also be a separate, detachable structure, such as a detachable assembly structure of a syringe and a syringe needle.
[0036] Regarding the specific structure of the needle body 12, it can be a puncture needle structure, that is, the end of the needle body 12 away from the main body 11 is a needle tip with a cut bevel structure. Of course, since the structure of the end of the needle body away from the main body 11 is designed to allow it to pierce the sampled tissue, the end of the needle body away from the main body 11 can also have other structures, such as a flat end. In order to allow it to pierce the sampled tissue, the wall thickness of the needle body is made thinner closer to the end opening. As for the size of the lumen 121, its diameter can be selected between 0.6mm and 1.6mm, specifically 0.8mm or 1.2mm. Of course, provided that the usage requirements are met, the diameter of the lumen 121 can also be less than 0.6mm or greater than 1.6mm.
[0037] The second seal 14 is used to seal the end of the needle body 12 away from the main body 11, and the first seal 13 is used to seal the opening of the homogenization chamber 111. After sampling is completed, the second seal 14 can be installed on the tube body to seal the end of the needle body 12 away from the main body 11, which is the end used to pierce the sampled tissue. After homogenization medium is added to the homogenization chamber 111, the opening can be sealed by the first seal 13, such as... Figure 1 As shown, homogenization can then be performed using the integrated sampling and homogenization device 1. Both sampling and homogenization are completed using this single device, simplifying the sampling and homogenization operations. Furthermore, since no tissue transfer is performed, tissue sample contamination can be minimized.
[0038] In one embodiment, please refer to Figure 2The second sealing element 14 includes a sealing cap 141 and a sealing block 142. The sealing cap 141 is a cup-shaped structure with an inner cavity. The sealing block 142 is disposed at the bottom of the inner cavity of the sealing cap 141. The sealing cap 141 is detachably and sealingly connected to the main body 11. The needle body 12 is covered inside the sealing cap 141. The sealing block 142 is made of silicone or medical-grade polymer material. After the sealing cap 141 is installed on the main body 11, the end of the needle body 12 presses against or penetrates the sealing block 142. The sealing block 142 elastically deforms, sealing the end of the needle body 12. This forms a two-stage seal for the needle body 12, ensuring the reliability of the seal.
[0039] Specifically, in one embodiment, the sealing block 142 is a cylindrical block structure. As described above, the needle body 12 is inserted into the sealing block 142 of the block structure, so that the sealing block 142 seals the end of the needle body 12. In other embodiments, the sealing block can also be a block structure of other shapes, such as a cube, a hexagonal prism, etc. Of course, the structure of the sealing block can also include a columnar part and an annular part provided around the root of the columnar part, and there is an annular gap between the annular part and the columnar part. When the needle body and the sealing block are pressed together, the end of the needle body 12 is inserted into the annular gap, the columnar part is inserted into the cavity 121 of the needle body 12 and is sealed with the inner wall of the cavity 121, and the annular part is sleeved on the outside of the needle body 12 and is sealed with the needle body 12.
[0040] Regarding the sealing connection between the sealing cap 141 and the main body 11, in one embodiment, the sealing cap 141 has an internal thread, and the main body 11 has an external thread. The sealing cap 141 is screwed onto the main body 11 for a sealing seal. In other embodiments, the sealing cap and the main body can also adopt other sealing connection methods, such as the sealing cap being inserted into the main body, with at least one sealing ring provided on the inner side of the sealing cap. The sealing ring protrudes inward relative to the inner wall of the sealing cap. When inserted, the sealing ring and the main body fit tightly together to achieve a seal. Of course, a sealing ring can also be provided on the outer circumferential surface of the main body, and the sealing cap and the sealing ring fit tightly together to achieve a sealing installation of the sealing cap on the main body.
[0041] To prevent tissue samples entering the lumen 121 from flowing back out of the needle body 12, in one embodiment, an anti-backflow structure is provided inside the lumen 121 to prevent tissue samples entering the lumen 121 from flowing back out of the lumen 121. In one embodiment, the anti-backflow structure is a barb 122 provided on the inner wall of the lumen 121, with the angle between the barb and the inner wall of the lumen 121 between 15° and 35°, such as 20° or 30°. In other embodiments, the anti-backflow structure can also be an elastic membrane provided on the inner wall of the lumen. The elastic membrane has a two-lobed structure. During sampling, the tissue sample in the lumen 121 can push open the elastic membrane and enter the homogenization chamber 111. After the sampling operation is completed, the elastic membrane springs back and closes to prevent the tissue sample from flowing back.
[0042] In one embodiment, please refer to Figure 2 The main body 11 includes a transition section 112 for connection with the needle body 12, and the transition section 112 has a tapered structure. In other embodiments, the main body may also be a flat-bottomed cylindrical structure, with the needle body 12 connected to the bottom of the main body.
[0043] For the specific form of the opening of the homogenization chamber 111, please refer to one embodiment. Figure 2 The main body 11 has a port 113 at one end away from the needle body 12, forming an opening. The first sealing element 13 is a detachable sealing cap that seals the port 113. The sealing cap is connected to the main body 11 via a flexible connecting piece 114. The sealing cap can adopt a snap-fit structure at the port 113, specifically referring to the structure of the sealing cap of a centrifuge tube in the prior art that snaps onto the port. This ensures that the overall airtightness of the integrated sampling and homogenizing device 1 reaches 99.9%, meeting ISO pollution control standards.
[0044] Of course, in other embodiments, the first sealing cap can also be threaded and sealed at the pipe opening 113. For the opening structure, in other embodiments, it can also be formed on the pipe wall of the main body 11.
[0045] Homogenizing beads 2 are contained within the homogenizing chamber 111 and are used for high-frequency vibration grinding of the tissue sample during homogenization. Their diameter is between 0.5 mm and 1.0 mm, and they can be glass beads, metal beads, or a combination of both. It should be noted that... Figure 1 The term "sampling and homogenizing device" is intended to indicate that the homogenizing chamber 111 contains homogenizing beads 2. In reality, when the integrated sampling and homogenizing device assembly is stationary, the homogenizing beads 2 will sink. However, during homogenization, they will be shaken and distributed within the homogenizing medium 3. The homogenizing beads 2 can be pre-added to the integrated sampling and homogenizing device during the manufacturing stage of the integrated sampling and homogenizing device assembly.
[0046] In one embodiment, the main body 11 of the integrated sampling and homogenizing device 1 is a 1.5ml centrifuge tube structure with a screw-on bottom cap. The screw-on bottom cap refers to the first sealing element 13 being threaded onto the main body 11. Its dimensions are designed to fit directly into the module of a high-throughput tissue homogenizer, enabling it to work with the high-throughput tissue homogenizer for high-efficiency tissue homogenization. Of course, in other embodiments, the integrated sampling and homogenizing device can also be used in conjunction with other equipment for homogenization, such as ultrasonic equipment, employing ultrasonic homogenization. Figure 1 and Figure 2As shown, in one embodiment, the bottom of the first seal 13 is an inverted frustum structure and is pointed. In other embodiments, the bottom of the first seal can also be a spherical structure, that is, the shape of the integrated sampling and homogenizing device is similar to a round-bottomed centrifuge tube. The size of the integrated sampling and homogenizing device can also be a homogenization chamber volume of 2ml or other specifications.
[0047] In one embodiment, the first seal 13 is detachably and sealably installed at the opening of the main body 11. After tissue homogenization is completed, the first seal 13 can be opened to extract the tissue homogenate. Afterward, the first seal 13 can be reinstalled to seal the homogenization chamber 111, and the integrated sampling and homogenization device 1 can be used directly as a container for refrigerated tissue homogenization for subsequent analysis and sample storage. In other embodiments, the first seal 13 can be a non-removable structure after installation. That is, after the homogenization medium is added and the first seal is installed, it is no longer removable. After homogenization is completed, the second seal can be opened, and the tissue homogenate can be squeezed out of the lumen 121 of the needle body 12 by squeezing the main body 11 to obtain tissue homogenate for subsequent experiments.
[0048] This utility model also provides an integrated sampling and homogenizing device, which has the same structure and usage as the integrated sampling and homogenizing device in the above-mentioned integrated sampling and homogenizing device assembly, and will not be described again here.
[0049] The above-described specific examples are for illustrative purposes only and are not intended to limit the scope of this invention. Those skilled in the art to which this invention pertains can make various simple deductions, modifications, or substitutions based on the concept of this invention.
Claims
1. A sampling and homogenization integrated device, characterized in that, include: The tube body includes a main body and a needle body. The main body has a homogenization chamber with an opening for adding a homogenization medium into the homogenization chamber. The needle body has a lumen for inserting into the tissue to be sampled so that the tissue to be sampled enters the lumen. The lumen and the homogenization chamber are connected so that the tissue to be sampled entering the lumen can enter the homogenization chamber. A first sealing element is used to seal the opening; The second seal is used to seal the end of the needle portion that is away from the main body portion.
2. The integrated sampling and homogenizing apparatus as described in claim 1, characterized in that, The second sealing element includes a sealing cap and a sealing block. The sealing block is disposed at the bottom of the inner cavity of the sealing cap. The sealing cap is detachably and sealingly connected to the main body and covers the needle body. The sealing block is used to seal the end of the needle body.
3. The integrated sampling and homogenization apparatus as described in claim 2, characterized in that, The sealing cap is threaded onto the main body.
4. The integrated sampling and homogenizing apparatus as described in any one of claims 1-3, characterized in that, The lumen has an anti-backflow structure to prevent the sampled tissue that has entered the lumen from flowing back out of the lumen.
5. The integrated sampling and homogenizing apparatus as described in claim 4, characterized in that, The anti-backflow structure is a barb installed on the inner wall of the tube, and the angle between the barb and the inner wall of the tube is between 15° and 35°.
6. The integrated sampling and homogenizing apparatus as described in any one of claims 1-3, characterized in that, The main body includes a transition section for connecting with the needle body, the transition section being a tapered structure.
7. The integrated sampling and homogenizing apparatus as described in any one of claims 1-3, characterized in that, The main body is provided with a tube opening at one end away from the needle body, and the tube opening constitutes the opening. The first sealing member is a sealing cap that can be detachably sealed to the opening, and the sealing cap is connected to the main body through a flexible connecting piece.
8. The integrated sampling and homogenizing apparatus as described in any one of claims 1-3, characterized in that, The diameter of the lumen is between 0.6 mm and 1.6 mm.
9. A sampling and homogenization integrated device assembly, characterized in that, The sampling and homogenizing apparatus according to any one of claims 1-8 further includes homogenizing beads contained in the homogenizing chamber, the homogenizing beads being used to grind the sampled tissue during homogenization.
10. The integrated sampling and homogenizing apparatus assembly as described in claim 9, characterized in that, The homogenized beads include at least one of glass beads and metal beads.