Auxiliary device for fixed dehydration before immunohistochemical frozen section

Abstract

The auxiliary device comprises an outer tube, an inner tube and a tube cover, the lower end of the outer tube is closed, a first opening is formed in the upper end of the outer tube, a through hole communicated with the interior of the inner tube is formed in the lower end of the inner tube, a second opening is formed in the upper end of the inner tube, and the first opening is communicated with the through hole. An annular boss abutting against the upper end of the outer pipe is arranged on the outer wall of the inner pipe, the lower end of the inner pipe is inserted into the outer pipe and extends to the middle of the outer pipe, the upper end of the inner pipe extends out of the outer pipe and is matched with the pipe cover, and a filter screen is arranged on the lower portion in the inner pipe. 4% paraformaldehyde is loaded into the outer tube in advance, so that the use amount of the 4% paraformaldehyde can be reduced, and subsequent volatilization can be reduced; the inner tube can be separated from the outer tube, so that the inner tube with tissues can be conveniently transferred to different steps for operation; and tissue samples in different groups can be conveniently taken through operation of a single tube.

Description

Technical Field

[0001] This utility model relates to experimental apparatus, and in particular to an auxiliary device for fixation and dehydration of frozen sections before immunohistochemistry. Background Technology

[0002] Currently, the fixation and dehydration steps used for immunohistochemical frozen sections include: 1) Fresh tissue is placed in a standard embedding cassette, which is fixed in 4% paraformaldehyde for 24 hours; 2) The embedding cassette is transferred to 15% sucrose for dehydration for 24 hours, then the 15% sucrose is replaced with 30% sucrose for another 24 hours; 3) The embedding cassette is removed from the bottle, the tissue is removed from the cassette, blotted dry with filter paper, and then frozen-embedded and sectioned for OCT. Standard embedding cassettes are relatively large, requiring a significant amount of fixative and dehydrating solution to submerge the tissue. Furthermore, the subsequent tissue drying process is inconvenient, and there is a risk of tissue falling out through the perforations in the embedding cassette. Summary of the Invention

[0003] The technical problem to be solved by this invention is to provide an auxiliary device for fixation and dehydration before immunohistochemical frozen sectioning, which can reduce the amount of fixative and dehydrating solution used and facilitate the transfer of tissue to different steps.

[0004] To solve the above-mentioned technical problems, the technical solution of this utility model is: an auxiliary device for fixation and dehydration of frozen sections before immunohistochemistry, comprising an outer tube, an inner tube, and a cap. The lower end of the outer tube is closed, and the upper end of the outer tube has a first opening. The lower end of the inner tube has a through hole communicating with its interior, and the upper end of the inner tube has a second opening. The outer wall of the inner tube has an annular protrusion that abuts against the upper end of the outer tube. The lower end of the inner tube is inserted into the outer tube and extends to the middle of the outer tube. The upper end of the inner tube extends out of the outer tube and cooperates with the cap. A filter screen is provided in the lower part of the inner tube. The principle of this invention is as follows: 1) In a fume hood, 4% paraformaldehyde is added to the outer tube in advance. Fresh tissue is placed into the inner tube, and the inner tube is placed into the outer tube. After the tube cap is closed, the device is inverted so that 4% paraformaldehyde enters the inner tube through the through hole and immerses the tissue for 24 hours; 2) The inner tube is removed from the outer tube and directly transferred to the outer tube containing 15% sucrose. After the tube cap is closed, the device is inverted so that 15% sucrose enters the inner tube through the through hole and immerses the tissue for 24 hours to dehydrate; 3) The inner tube is removed from the outer tube and directly transferred to the outer tube containing 30% sucrose. After the tube cap is closed, the device is inverted so that 30% sucrose enters the inner tube through the through hole and immerses the tissue for 24 hours to dehydrate; 4) The device is centrifuged at low speed in a centrifuge to dry the tissue. The tissue is then removed and cryogenically embedded and sectioned using OCT.

[0005] As an improvement, the pipe cover includes a cover plate and a circular tube disposed at the bottom of the cover plate, the circular tube being able to be inserted into a second opening at the upper end of the inner tube.

[0006] As an improvement, the pipe cap is connected to the outer pipe via a connecting strap.

[0007] The beneficial effects of this utility model compared with the prior art are:

[0008] Preloading 4% paraformaldehyde into the outer tube can reduce the amount of 4% paraformaldehyde used and reduce subsequent volatilization; the inner tube can be detached from the outer tube, making it convenient for the inner tube to transfer tissue to different steps; single-tube operation makes it convenient to pick up tissue samples in different groups. Attached Figure Description

[0009] Figure 1 This is a schematic diagram of the inner and outer tubes combined.

[0010] Figure 2 This is a schematic diagram showing the disassembly of the inner and outer tubes.

[0011] Figure 3 Diagram I shows the usage status of the auxiliary device.

[0012] Figure 4 Diagram II shows the usage status of the auxiliary device. Detailed Implementation

[0013] The present invention will be further described below with reference to the accompanying drawings.

[0014] like Figure 1 , 2 As shown, an auxiliary device for fixation and dehydration of frozen sections before immunohistochemistry includes an outer tube 1, an inner tube 21, and a cap 3. The lower end of the outer tube 1 is closed, and the upper end of the outer tube 1 has a first opening. The lower end of the inner tube 21 has a through hole 21 communicating with its interior. A filter screen 22 is provided in the lower part of the inner tube 21, allowing external liquid to enter the inner tube 21 through the through hole 21 and the filter screen 22. The filter screen 22 prevents tissue inside the inner tube 21 from falling out through the through hole 21. The upper end of the inner tube 21 has a second opening, and the outer wall of the inner tube 21 has an annular protrusion 23 that abuts against the upper end of the outer tube 1. The lower end of the inner tube 21 is inserted into the outer tube 1 and extends to the middle of the outer tube 1. After the inner tube 21 and the outer tube 1 are engaged, the annular protrusion 23 abuts against the upper end of the outer tube 1, and the liquid inside the outer tube 1 is below the inner tube 21. Only when the outer tube 1 is inverted can the liquid inside it enter the inner tube 21. The upper end of the inner tube 21 extends out of the outer tube 1 and cooperates with the tube cover 3. The tube cover 3 includes a cover plate 31 and a round tube 32 located at the bottom of the cover plate 31. The round tube 32 can be inserted into the second opening at the upper end of the inner tube 21. The tube cover 3 is connected to the outer tube 1 by a connecting strap 33.

[0015] like Figure 3 , 4As shown, the principle of this utility model is as follows: 1) In a fume hood, 4% paraformaldehyde is added to the outer tube 1 in advance. Fresh tissue is placed into the inner tube 21, and the inner tube 21 is placed into the outer tube 1. After the tube cap 3 is closed, the device is inverted so that 4% paraformaldehyde enters the inner tube 21 through the through hole 21 and immerses the tissue for 24 hours; 2) The inner tube 21 is removed from the outer tube 1 and directly transferred to the outer tube 1 containing 15% sucrose. After the tube cap 3 is closed, the device is inverted so that 15% sucrose enters the inner tube 21 through the through hole 21 and immerses the tissue for 24 hours to dehydrate; 3) The inner tube 21 is removed from the outer tube 1 and directly transferred to the outer tube 1 containing 30% sucrose. After the tube cap 3 is closed, the device is inverted so that 30% sucrose enters the inner tube 21 through the through hole 21 and immerses the tissue for 24 hours to dehydrate; 4) The device is centrifuged at low speed in a centrifuge to dry the tissue. The tissue is then removed and frozen-embedded and sectioned using OCT.

[0016] Preloading 4% paraformaldehyde into the outer tube 1 can reduce the amount of 4% paraformaldehyde used and reduce subsequent volatilization; the inner tube 21 can be detached from the outer tube 1, making it convenient for the inner tube 21 to transfer tissue to different steps; single-tube operation makes it convenient to pick up tissue samples in different groups.

Claims

1. An auxiliary device for fixation and dehydration before immunohistochemical frozen sectioning, characterized in that: The device includes an outer tube, an inner tube, and a cap. The lower end of the outer tube is closed, and the upper end of the outer tube has a first opening. The lower end of the inner tube has a through hole communicating with its interior, and the upper end of the inner tube has a second opening. The outer wall of the inner tube has an annular protrusion that abuts against the upper end of the outer tube. The lower end of the inner tube is inserted into the outer tube and extends to the middle of the outer tube. The upper end of the inner tube extends out of the outer tube and cooperates with the cap. A filter screen is provided in the lower part of the inner tube.

2. The auxiliary device for fixation and dehydration before immunohistochemical frozen sections according to claim 1, characterized in that: The pipe cover includes a cover plate and a circular tube located at the bottom of the cover plate, the circular tube being able to be inserted into a second opening at the upper end of the inner tube.

3. The auxiliary device for fixation and dehydration before immunohistochemical frozen sections according to claim 1, characterized in that: The pipe cap is connected to the outer pipe via a connecting strap.

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