Efficient colloidal gold detection card

Abstract

The utility model relates to the field of reagent detection equipment, in particular to an efficient colloidal gold detection card which comprises a support bottom, a bottom plate, a sample pad, an isolation pad, a gold label pad and a chromatographic membrane, the support bottom is used for supporting the structure of the whole detection card; the bottom plate is made of a PVC (Polyvinyl Chloride) material and is adhered to the support bottom; the sample pad is adhered to one end of the bottom plate and is used for receiving a sample to be detected; the gold mark pad is pasted on the bottom plate and attached to the sample pad, and the sample pad contains a specific antibody marked by colloidal gold and is used for being combined with a target substance; the sample pad and the receiving pad are attached to the gold-labeled pad, and a specific antibody labeled by colloidal gold in the gold-labeled pad ensures high-sensitivity capture of a target substance in the detection process. Due to the arrangement of a detection line and a quality control line on the chromatography film, the accuracy and the reliability of a detection result are further improved, and due to the design of a flow guide channel and a flow dividing channel in the upper cover, samples can be accurately and uniformly distributed to the sample pad and the receiving pad.

Description

Technical Field

[0001] This utility model relates to the field of reagent testing equipment, specifically a high-efficiency colloidal gold detection card. Background Technology

[0002] Currently, colloidal gold test strips, as an important bioanalytical tool, have shown broad application prospects in various fields such as bioanalysis, drug residue detection, and food safety monitoring. Their unique advantages, based on colloidal gold immunochromatography technology, enable rapid and intuitive result interpretation without the need for complex equipment. However, with the continuous advancement of modern detection technologies and the increasing demands of applications, traditional colloidal gold test strips have gradually revealed some problems, limiting their further development and application.

[0003] First, traditional colloidal gold assay cards suffer from low detection sensitivity. When the concentration of the target analyte in the sample is low, traditional assay cards often fail to accurately capture the target signal, resulting in unsatisfactory detection results. This not only affects the accuracy and reliability of the detection but also increases the risk of missed and false detections.

[0004] Secondly, the long reaction time is also a significant drawback of traditional colloidal gold detection cards. In the field of rapid detection, time is efficiency. An excessively long reaction time not only prolongs the detection cycle but also increases the possibility of interference factors during the detection process, further affecting the accuracy of the detection results.

[0005] Furthermore, the complexity of operation is another aspect that traditional colloidal gold assay cards need improvement. Although colloidal gold immunochromatography technology itself offers a certain degree of ease of operation, traditional assay cards are often not designed with user experience in mind, resulting in cumbersome procedures and a high risk of errors. This not only increases the workload of testing personnel but also reduces testing efficiency. Utility Model Content

[0006] (a) Technical problems to be solved

[0007] To address the shortcomings of existing technologies, this invention provides a high-efficiency colloidal gold detection card.

[0008] (II) Technical Solution

[0009] To achieve the above objectives, the present invention provides the following technical solution: A high-efficiency colloidal gold detection card of the present invention includes a base, a base plate, a sample pad, a separating pad, a gold label pad, and a chromatography membrane;

[0010] Base: The structure used to support the entire test card;

[0011] Base plate: Made of PVC material, glued to the base plate.

[0012] Sample pad: Adhesive to one end of the base plate to receive the sample to be tested;

[0013] Gold-labeled pad: Adhesive to the base plate and attached to the sample pad, the sample pad containing colloidal gold-labeled specific antibodies for binding to the target substance;

[0014] The isolation pad is made of water-soluble film and is attached between the sample pad and the gold label pad to isolate the sample pad and the gold label pad.

[0015] Chromatographic membrane: Adhered to the gold standard pad, it is a nitrocellulose membrane with a backing, on which test lines and control lines are set to display the test results;

[0016] Top cover: The top cover is attached to the base, one end of the top cover is provided with a sample inlet, the sample inlet is adapted to the sample pad, and the top cover is provided with an observation port, which is adapted to the gold label pad;

[0017] A receiving pad is attached to the gold label pad, and the receiving pad is located between the detection line and the quality control line;

[0018] The upper cover has a flow guiding channel inside, the sample inlet is connected to the flow guiding channel, the flow guiding channel has a first flow splitting channel and a second flow splitting channel, the first flow splitting channel is connected to the receiving pad, and the second flow splitting channel is connected to the sample pad;

[0019] The upstream end of the flow guiding channel smoothly transitions to the downstream end of the flow guiding channel.

[0020] Preferably, an absorbent pad is attached to the base plate, and the sample pad, gold label pad, and isolation pad are all attached to the absorbent pad.

[0021] More preferably, the sample pad and the receiving pad are made of either glass cellulose film or polyester film.

[0022] (III) Beneficial Effects

[0023] Compared with the prior art, this utility model provides a high-efficiency colloidal gold detection card, which has the following beneficial effects:

[0024] Sturdy structure and easy operation:

[0025] The combined design of the base and bottom plate provides a stable support structure for the test card, ensuring it is not easily deformed or damaged during testing. Meanwhile, the top cover design simplifies and speeds up sample introduction and result observation, improving user convenience.

[0026] High sensitivity and accuracy:

[0027] The precise fit between the sample pad and receiver pad and the gold-labeled pad, along with the specific antibody labeled with colloidal gold in the gold-labeled pad, ensures highly sensitive capture of the target substance during the detection process. The detection lines and control lines on the chromatography membrane further enhance the accuracy and reliability of the detection results.

[0028] Sample isolation and reaction efficiency:

[0029] The introduction of the isolation pad effectively separates the sample pad and the gold-labeled pad, preventing the sample from flowing directly to the gold-labeled pad before it has fully reacted, thus ensuring the sufficiency and efficiency of the reaction. Furthermore, the adhesive design of the absorbent pad further enhances the uniform distribution and flow control of the sample.

[0030] The test results are intuitive and easy to read:

[0031] The test lines and control lines on the chromatography membrane directly display the test results through color changes, eliminating the need for complex instrument analysis and making the results intuitive and easy to read. This is especially important for non-professionals or for rapid on-site testing.

[0032] Optimize the diversion and distribution design:

[0033] The internal flow channels and distribution channels of the top cover allow for precise and uniform distribution of samples onto the sample pad and receiving pad, improving the accuracy and consistency of the detection. Simultaneously, the smooth transition of the flow channels reduces resistance to sample flow, accelerating the detection speed.

[0034] Ingredient optimization and stability improvement:

[0035] The gold-labeled pad contains sucrose, disodium hydrogen phosphate dodecahydrate, bovine serum albumin, sodium caseinate, trehalose, and polyethylene glycol, which help stabilize the colloidal gold-labeled antibody and improve the stability and repeatability of the detection.

[0036] Diversity and applicability of material selection:

[0037] The sample pad and receiving pad are made of materials such as glass fiber membrane or polyester membrane, which have good water absorption, permeability and biocompatibility, making them suitable for testing a variety of sample types. At the same time, the diverse material selection increases the applicability and flexibility of the test card.

[0038] In summary, the high-efficiency colloidal gold test card provides users with a convenient, accurate, and reliable testing tool through its unique structural design, highly sensitive detection mechanism, intuitive display of test results, and optimized selection of components and materials. Attached Figure Description

[0039] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0040] Figure 2 This is a schematic diagram of the detection part of this utility model;

[0041] Figure 3 This is a schematic diagram of the cross-sectional structure of the upper cover of this utility model;

[0042] In the diagram: 1. Base; 2. Top cover; 3. Base plate; 4. Absorbent pad; 5. Sample pad; 6. Chromatography membrane; 7. Isolation pad; 8. Receiving pad; 9. Detection line; 10. Quality control line; 11. Observation port; 12. Inlet port; 13. Flow channel; 14. Flow channel one; 15. Flow channel two; 16. Gold label pad. Detailed Implementation

[0043] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0044] Please see Figure 1-3 The present invention provides a high-efficiency colloidal gold detection card, comprising a base 1, a base plate 3, a sample pad 5, a separating pad 7, a gold label pad 16, and a chromatography membrane 6.

[0045] Base 1: A structure used to support the entire detection card;

[0046] Base plate 3: Made of PVC material, it is attached to base plate 1.

[0047] Sample pad 5: Attached to one end of the base plate 3, used to receive the sample to be tested;

[0048] Gold-labeled pad 16: Adhesive to the base plate 3 and attached to the sample pad 5, wherein the sample pad 5 contains colloidal gold-labeled specific antibodies for binding to the target substance;

[0049] Isolation pad 7: Made of water-soluble film, the isolation pad 7 is attached between the sample pad 5 and the gold label pad 16 to isolate the sample pad 5 and the gold label pad 16;

[0050] Chromatography membrane 6: Adhered to the gold standard pad 16, it is a nitrocellulose membrane with a backing, and has a detection line 9 and a control line 10 on it to display the detection results;

[0051] Top cover 2: The top cover 2 is attached to the base 1. One end of the top cover 2 is provided with a sample inlet 12, which is adapted to the sample pad 5. The top cover 2 is provided with an observation port 11, which is adapted to the gold label pad 16.

[0052] A receiving pad 8 is attached to the gold label pad 16, and the receiving pad 8 is located between the detection line 9 and the quality control line 10.

[0053] The upper cover 2 has a flow guiding channel 13 inside, the sample inlet 12 is connected to the flow guiding channel 13, the flow guiding channel 13 is provided with a first flow dividing channel 14 and a second flow dividing channel 15, the first flow dividing channel 14 is connected to the receiving pad 8, and the second flow dividing channel 15 is connected to the sample pad 5.

[0054] The upstream end of the flow guiding channel 13 smoothly transitions to the downstream end of the flow guiding channel 13.

[0055] The preferred solution for the aforementioned high-efficiency colloidal gold detection card is:

[0056] The combination of base plate 3 and absorbent pad 4

[0057] Working principle: An absorbent pad 4 is attached to the base plate 3, which serves as the liquid absorption and storage part of the entire detection system. When the sample flows through the detection card, excess liquid is absorbed by the absorbent pad 4, maintaining the humidity balance inside the detection card and preventing liquid from overflowing.

[0058] Adhesion of sample pad 5, gold label pad 16 and isolation pad 7

[0059] Working principle: The sample pad 5, gold label pad 16, and isolation pad 7 are all attached to the absorbent pad 4, ensuring a tight connection and positioning between them. The sample pad 5 receives the sample, the isolation pad 7 controls the liquid flow rate, and the gold label pad 16 is responsible for binding with the target substance.

[0060] Introduction of receiving pad 8

[0061] Working principle: A receiving pad 8 is attached to the gold label pad 16, located between the test line 9 and the control line 10. The receiving pad 8 can further capture and immobilize the antigen-antibody complex, improving the accuracy and stability of the test.

[0062] Design of upper cover 2 guide channels 13

[0063] Working principle: The upper cover 2 has a flow guiding channel 13 inside, which is connected to the sample inlet 12. The first flow splitting channel 14 and the second flow splitting channel 15 on the flow guiding channel 13 guide the sample to the sample pad 5 and the receiving pad 8, respectively. This design ensures uniform distribution and rapid flow of the sample, improving detection efficiency.

[0064] Smooth transition: The upstream end of the flow channel 13 smoothly transitions to the downstream end of the flow channel 13, reducing resistance and bubble generation during liquid flow and ensuring smooth sample flow.

[0065] Ingredient ratio of Gold Standard Pad 16

[0066] Working principle: In addition to the colloidal gold-labeled specific antibody, the Gold Label Pad 16 also contains sucrose, disodium hydrogen phosphate dodecahydrate, bovine serum albumin, sodium caseinate, trehalose, and polyethylene glycol. These components play important roles in protecting antigens and antibodies, improving the stability of colloidal gold, and regulating osmotic pressure, thereby enhancing the sensitivity and stability of the test card.

[0067] Material selection for sample pad 5 and receiving pad 8

[0068] Working principle: Sample pad 5 and receiving pad 8 are made of either glass cellulose membrane or polyester membrane. These materials have good liquid absorption and diffusion properties, enabling them to quickly and effectively receive and transfer samples and reaction products, ensuring the accuracy and reliability of the detection.

[0069] The high-efficiency colloidal gold assay card is a portable testing tool integrating multiple components. Its working principle is based on the combination of colloidal gold labeling technology and chromatographic analysis. The following is a detailed summary of the components of this assay card and their working principles:

[0070] Bottom line 1:

[0071] Function: As the supporting structure of the entire test card, it ensures that all components can be firmly fixed together and maintains the overall shape and stability of the test card.

[0072] Base plate 3 (PVC material):

[0073] Function: Made of PVC material, its excellent physical and chemical stability makes it suitable as a base for test cards. The base plate 3 is attached to the support plate 1, providing a flat and stable attachment surface for other components.

[0074] Sample pad 5:

[0075] Function: Attached to one end of the base plate 3, this is the starting part of the test card. Its function is to receive the sample to be tested, distribute the sample evenly through capillary action, and guide it to the subsequent detection area.

[0076] Gold Label Pad 16:

[0077] Function: Adhesive to sample pad 5, containing colloidal gold-labeled specific antibodies. As the sample flows through the gold-labeled pad 16, the target substance in the sample binds to the colloidal gold-labeled antibody, forming an antigen-antibody complex.

[0078] Isolation Pad 7 (Water-soluble film):

[0079] Function: Located between sample pad 5 and gold label pad 16, it acts as an separator. It effectively prevents the sample from flowing directly to the gold label pad 16 before it has fully reacted, ensuring the sufficiency and accuracy of the reaction. Simultaneously, the water-soluble film can dissolve under specific conditions, facilitating the observation of subsequent results.

[0080] Chromatography membrane 6 (backed nitrocellulose membrane):

[0081] Function: Adhered to the gold label pad 16, this is the core component of the test card. The chromatography membrane 6 is equipped with a detection line 9 and a control line 10, used to capture and display the presence of specific antigen-antibody complexes and the effectiveness of the detection process, respectively. As the antigen-antibody complexes move across the chromatography membrane 6, they form visible bands on the detection line 9, indicating the presence or absence of the target substance.

[0082] Top cover 2:

[0083] Function: Attached to the base 1, it provides protection for the test card and guides the addition of the sample. One end of the top cover 2 has a sample inlet 12, which fits into the sample pad 5, facilitating the user to add the sample to the test card. Simultaneously, the top cover 2 has an observation port 11, which fits into the gold standard pad 16, allowing the user to observe the test results on the chromatography membrane 6 through this window after the test is completed. The inlet can be sealed with a plug during normal use; the plug can be threaded to connect to the inlet.

[0084] In summary, the working principle of the high-efficiency colloidal gold detection card is as follows: the sample pad 5 receives the sample to be tested; the target substance in the sample binds to the colloidal gold-labeled antibody on the gold-labeled pad 16 to form a complex; the complex moves on the chromatography membrane 6 and forms a visible band on the detection line 9, thereby achieving rapid and sensitive detection of the target substance. The entire detection process is simple and fast, the results are intuitive and easy to read, and it is suitable for a variety of application scenarios.

[0085] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A high-efficiency colloidal gold detection card, characterized in that, It includes a base (1), a base plate (3), a sample pad (5), a separation pad (7), a gold label pad (16), and a chromatography membrane (6); Support (1): A structure used to support the entire detection card; Base plate (3): Made of PVC material, it is attached to the base plate (1). Sample pad (5): Attached to one end of the base plate (3) to receive the sample to be tested; Gold label pad (16): It is pasted on the base plate (3) and attached to the sample pad (5). The sample pad (5) contains colloidal gold-labeled specific antibodies for binding to the target substance. Isolation pad (7): A water-soluble film is used. The isolation pad (7) is attached between the sample pad (5) and the gold label pad (16) to isolate the sample pad (5) and the gold label pad (16). Chromatographic membrane (6): Adhered to the gold standard pad (16), it is a nitrocellulose membrane with a backing, on which a detection line (9) and a quality control line (10) are set to display the detection results; Top cover (2): The top cover (2) is pasted on the base (1). One end of the top cover (2) is provided with a sample inlet (12). The sample inlet (12) is adapted to the sample pad (5). The top cover (2) is provided with an observation port (11). The observation port (11) is adapted to the gold label pad (16). A receiving pad (8) is attached to the gold label pad (16), and the receiving pad (8) is located between the detection line (9) and the quality control line (10); The upper cover (2) is provided with a flow guide channel (13) inside. The sample inlet (12) is connected to the flow guide channel (13). The flow guide channel (13) is provided with a first flow divider channel (14) and a second flow divider channel (15). The first flow divider channel (14) is connected to the receiving pad (8), and the second flow divider channel (15) is connected to the sample pad (5). The upstream end of the flow channel (13) smoothly transitions to the downstream end of the flow channel (13).

2. The high-efficiency colloidal gold detection card according to claim 1, characterized in that, A water-absorbing pad (4) is attached to the base plate (3), and the sample pad (5), gold label pad (16) and isolation pad (7) are all attached to the water-absorbing pad (4).

3. The high-efficiency colloidal gold detection card according to claim 2, characterized in that, The sample pad (5) and the receiving pad (8) are made of either glass cellulose film or polyester film.

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