PCR (Polymerase Chain Reaction) detection equipment

Abstract

The PCR detection equipment comprises a box body, a transverse plate, PCR reaction tubes, a display screen, a control area, a ventilation opening, hydraulic devices, an LED light source and a sample frame, the display screen is fixedly arranged on the front face of the box body, the control area is fixedly arranged under the display screen, the hydraulic devices are arranged on the two sides of the top of the box body, and the transverse plate is fixedly arranged at the top ends of the hydraulic devices; the sample frame is arranged in an empty groove formed in the middle of the top of the box body, the PCR reaction tube is arranged on the sample frame, the ventilation openings are formed in the two sides of the box body, and the LED light source is fixedly arranged at the bottom of the transverse plate. Efficient temperature control is achieved through precise cooperation of the heating pipe, the heat conduction beads, the fan and the temperature control valve; the photoelectric detector, the optical filter and the optical lens system improve the sensitivity of fluorescence signal detection; meanwhile, the equipment is compact in structure and easy and convenient to operate, and the maintenance cost is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to PCR detection technical field, concretely is a PCR detection equipment. BACKGROUND

[0002] In the prior art, PCR (polymerase chain reaction) detection equipment has been widely used, but there are still some deficiencies. Specifically, the heating and temperature control system of the existing equipment is not accurate and efficient enough, resulting in unstable temperature control during the PCR reaction process, which further affects the reaction efficiency and result accuracy. In addition, the existing equipment has the problem of insufficient sensitivity in fluorescence signal detection, making it difficult to accurately capture and analyze weak fluorescence signals. At the same time, the complexity of the operation and the maintenance cost of the equipment are relatively high, which is not conducive to its popularization and application in the laboratory. SUMMARY

[0003] The utility model aims at providing a PCR detection equipment to solve the above problems.

[0004] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a PCR detection equipment, including box, transverse plate, PCR reaction tube, display screen, control area, vent, hydraulic device, LED light source and sample rack, the front of box is fixed with display screen, the control area is fixed below display screen, the hydraulic device sets up at the top of both sides of box, the transverse plate is fixed on the top of hydraulic device, the sample rack is arranged in the empty groove arranged in the middle position of the top of box, the PCR reaction tube is arranged on the sample rack, the vent is opened in the both sides of box, the LED light source is fixed at the bottom of transverse plate.

[0005] Preferably, the control area includes control buttons, switches and safety devices, the bottom of the transverse plate is fixed with a limiting piece, which surrounds the LED light source, limiting the LED light source to emit light vertically downward, and a valve is fixed in the vent.

[0006] Preferably, the PCR reaction tube includes a cap, a connecting neck, a tube body, a heat-conducting bead and a support frame, the sample rack is internally provided with a plurality of sample chambers, the support frame is arranged in the sample chamber, the tube body is fixed below the support frame, the support frame is fixed with the connecting neck, and the cap is connected to the connecting neck, and the tube body is fixed with the heat-conducting bead at the bottom.

[0007] Preferably, a plurality of fixing buckles are fixed on the sample rack, and a heating tube is fixed below the sample chamber, and the heating tube is fixed at both ends on the inner wall of the empty groove at the position.

[0008] Preferably, the fixing buckle includes a first support and a hook, the support is fixed on the sample rack, and a second support is fixed below the heating tube.

[0009] Preferably, a fan is arranged below the second support, a connecting piece is arranged in the second support, a fan blade is arranged below the connecting piece, the fan blade is arranged on a rotating shaft below it, a motor is arranged below the rotating shaft, and the motor drives the rotating shaft to rotate.

[0010] Preferably, a photoelectric detector is fixed below the motor, a plurality of air slots are formed in the photoelectric detector, a filter is fixed in the air slot, and an optical lens system is fixed in the photoelectric detector close to the filter.

[0011] Compared with the prior art, the present application has the following advantages:

[0012] 1. By cooperating the heating pipe with the heat-conducting beads, and adjusting the fan and the temperature control valve, the reaction liquid in the PCR reaction tube can be accurately and efficiently heated and temperature-controlled. The heat-conducting beads have high heat-conducting performance, can quickly transfer heat to the reaction liquid, and ensure that the entire reaction system can be uniformly heated in a short time. At the same time, the fan and the temperature control valve can quickly raise and lower the temperature according to the needs, and meet the strict requirements of PCR reaction on temperature control;

[0013] 2. By cooperating the photoelectric detector, the filter and the optical lens system, the sensitivity of the fluorescence signal detection is improved. The filter can selectively absorb, reflect or transmit light of different wavelengths, so that only the fluorescence signal of a specific wavelength can reach the photoelectric detector. The optical lens system can further focus the fluorescence signal, and improve the accuracy of signal detection;

[0014] 3. The device has compact structure and reasonable design, so that the operation of the device is more convenient. At the same time, the connection and fixing mode between the various components are optimized, and the maintenance cost of the device is reduced. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is a structural schematic view of the present application;

[0016] Figure 2 It is a front view of the present application;

[0017] Figure 3 It is a schematic view of the fixing buckle of the present application;

[0018] Figure 4 It is a front sectional view of the present application;

[0019] Figure 5 It is a front sectional view of the fan of the present application;

[0020] Figure 6 It is a schematic view of the LED light source of the present application;

[0021] Figure 7 It is the schematic view of the PCR reaction tube of the utility model;

[0022] Figure 8 It is the top view of the fan of the utility model.

[0023] In the figure: box 1, cross plate 2, PCR reaction tube 3, pipe cap 301, connecting neck 302, pipe body 303, heat-conducting bead 304, support frame 305, display screen 4, control area 5, control button 501, switch 502, safety device 503, air vent 6, valve 601, hydraulic device 7, LED light source 8, limiting piece 801, sample holder 9, sample chamber 901, fixing buckle 11, first support 1101, hook 1102, heating pipe 12, fan 13, connecting piece 1301, fan blade 1302, rotating shaft 1303, motor 1304, second support 1305, optical filter 14, photoelectric detector 15. DETAILED DESCRIPTION

[0024] The utility model will be described below in conjunction with the drawings in the embodiment of the utility model Figures 1-8 The technical scheme in the embodiment of the utility model is clearly and completely described, obviously, the described embodiment is only a part of the embodiment of the utility model, not all the embodiment. Based on the embodiment in the utility model, all other embodiments obtained by the person skilled in the art without making creative work belong to the protection scope of the utility model.

[0025] The person skilled in the art without making creative work, all other embodiments obtained by the person skilled in the art without making creative work belong to the protection scope of the utility model.

[0026] The protection scope of the utility model.

[0027] Please refer to Figures 1-8 The utility model provides a kind of technical scheme: a kind of PCR detection equipment, including box 1, cross plate 2, PCR reaction tube 3, display screen 4, control area 5, air vent 6, hydraulic device 7, LED light source 8 and sample holder 9, box 1 front is fixed with display screen 4 by screwing, control area 5 is fixed below display screen 4 by screwing, hydraulic device 7 is set in the top of box 1 two sides by screwing, cross plate 2 is fixed on the top of hydraulic device 7 by screwing, sample holder 9 can be fixed by bolt and be set in the empty slot of the middle position of the top of box 1, PCR reaction tube 3 is set on sample holder 9, air vent 6 is set in the position of the two sides of box 1, LED light source 8 is fixed in the bottom of cross plate 2 by screwing.

[0028] The control area 5 includes a control button 501, a switch 502, and a safety device 503, which is a safety device such as a fuse. When the current is too large and exceeds the rated current of the fuse, the fuse is melted due to heat, thereby cutting off the circuit to achieve the protection effect of the circuit. The bottom of the horizontal plate 2 is fixed with a limiting piece 801, which surrounds the LED light source 8 to limit the LED light source 8 to emit light vertically downward. The vent 6 is fixed with a valve 601, which is a temperature control valve. During the PCR reaction process, the vent is closed by closing the valve 601, and the heating pipe 12 is opened to meet the heating requirement. When cooling is needed, the valve 601 is opened to dissipate heat from the vent 6.

[0029] The PCR reaction tube 3 includes a tube cap 301, a connecting neck 302, a tube body 303, a heat-conducting bead 304, and a support frame 305. The sample holder 9 is provided with a plurality of sample chambers 901, and the support frame 305 is arranged in the sample chamber 901. The tube body 303 is fixed below the support frame 305, and the connecting neck 302 is fixed on the support frame 305 and is made of plastic and can be bent. The connecting neck 302 is connected with the tube cap 301, and the tube body 303 is fixed with the heat-conducting bead 304 at the bottom. The heat-conducting bead 304 is made of high-thermal-conductivity materials such as metal, alloy, or some non-metallic materials (graphite, carbon nanotubes, etc.). The heat-conducting bead 304 quickly transfers the heat generated by the heating pipe 12 to the reaction solution in the PCR reaction tube 3 through its high-thermal-conductivity, ensuring that the entire reaction system is uniformly heated in a short time. At the same time, the heat-conducting bead 304 acts as a medium for heat transfer and can respond to the adjustment of the temperature control system such as the heating pipe 12 and the fan 13 to achieve rapid heating and cooling. In addition, during the PCR reaction process, if the temperature is too high or too low, it may cause damage to the enzyme activity and DNA template. The heat-conducting bead 304 can play a role in heat buffering to a certain extent to protect the reaction solution from extreme temperatures.

[0030] The sample holder 9 is fixed with a plurality of fixing buckles 11 by welding. The heating pipe 12 is fixed below the sample chamber 901 by screwing.

[0031] The fixing buckle 11 includes a first support 1101 and a hook 1102. The first support 1101 is fixed on the sample holder 9 by welding. The first support 1101 and the hook 1102 are made of plastic. The hook 1102 can be fixed by pressing the first support 1101 to fix the PCR reaction tube and the sample holder 9. The second support 1305 is fixed below the heating pipe 12 by screwing.

[0032] A fan 13 is arranged below the second support, a connecting piece 1301 and a bearing are arranged in the second support 1305, the connecting piece 1301 is connected with the bearing arranged in the second support 1305, a fan blade 1302 is arranged below the connecting piece 1301, the fan blade 1302 is arranged on a rotating shaft 1303 below the fan blade 1302 in a screwing mode, a motor 1304 is arranged below the rotating shaft 1303, and the motor 1304 drives the rotating shaft 1303 to rotate.

[0033] The photoelectric detector 15 is fixedly arranged below the motor 1304 in a screwing mode, a plurality of air slots are formed in the photoelectric detector 15, and a filter 14 is fixedly arranged in the air slots, the filter 14 is made of transparent materials such as optical glass, optical plastic or crystal, the materials have specific optical properties and can selectively absorb, reflect or transmit light of different wavelengths, and the filter 14 is used in cooperation with the photoelectric detector 15, when the fluorescent signal passes through the filter 14, the fluorescent signal is received by the photoelectric detector 15 and converted into an electric signal, and then signal processing and result analysis are performed, an optical lens system is fixedly arranged in the photoelectric detector 15 and close to the filter 14, the optical lens system is made of optical glass or optical plastic with high light transmittance, low dispersion and high refractive index, when the fluorescent signal passes through the filter 14, the optical lens system further focuses the fluorescent signal, and ensures that the signal can be accurately projected on the photoelectric detector 15, the sensitivity of signal detection can be improved, the accuracy of the signal can be ensured, the precise analysis of the PCR reaction result can be realized, and the interference of stray light and background light can be reduced, and the signal detection quality is improved.

[0034] In actual operation, the equipment is started through the switch 502 of the control area 5, the sample is placed in the PCR reaction tube 3, the PCR reaction tube 3 is fixed in the sample chamber 901 of the sample holder 9 through the support frame 305, the sample holder 9 is arranged in the air slot on the top of the box body 1 and is ensured to be stable through the fixing buckle 11, then the hydraulic device 7 drives the horizontal plate 2 to descend, and the LED light source 8 vertically irradiates on the PCR reaction tube 3, at the same time, the heating pipe 12 below the sample chamber 901 starts to work and heats the sample, during the PCR reaction process, the fan 13 drives the fan blade 1302 to rotate through the motor 1304, and ventilation and heat dissipation are realized, the fluorescent signal generated in the reaction is screened through the filter 14, focused by the optical lens system and projected on the photoelectric detector 15, and signal detection and analysis are performed.

[0035] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A PCR detection device, comprising a box (1), a transverse plate (2), a PCR reaction tube (3), a display screen (4), a control area (5), a ventilation opening (6), a hydraulic device (7), an LED light source (8), and a sample rack (9), characterized in that: The box (1) is provided with a display screen (4) on the front side, the control area (5) is fixedly arranged below the display screen (4), the hydraulic device (7) is arranged on the top of the box (1), the horizontal plate (2) is fixedly arranged on the top of the hydraulic device (7), the sample rack (9) is arranged in the groove arranged at the middle position of the top of the box (1), the PCR reaction tube (3) is arranged on the sample rack (9), the air vent (6) is arranged on the two sides of the box (1), and the LED light source (8) is fixedly arranged on the bottom of the horizontal plate (2).

2. The PCR detection device of claim 1, wherein: The control area (5) comprises a control button (501), a switch (502) and a safety device (503), the bottom of the horizontal plate (2) is fixedly provided with a limiting piece (801), the limiting piece (801) surrounds the LED light source (8) on the four sides, and the LED light source (8) can only emit light vertically downward, and the air vent (6) is fixedly provided with a valve (601).

3. The PCR detection device of claim 1, wherein: The PCR reaction tube (3) comprises a cap (301), a connecting neck (302), a tube body (303), a heat-conducting bead (304) and a support frame (305), the sample rack (9) is internally provided with a plurality of sample chambers (901), the support frame (305) is arranged in the sample chamber (901), the tube body (303) is fixedly arranged below the support frame (305), the connecting neck (302) is fixedly arranged on the support frame (305), the cap (301) is connected to the connecting neck (302), and the tube body (303) is fixedly provided with the heat-conducting bead (304) on the bottom.

4. The PCR detection device of claim 3, wherein: A plurality of fixing buckles (11) are fixedly arranged on the sample rack (9), and a heating pipe (12) is fixedly arranged below the sample chamber (901) and fixed to the inner wall of the groove at the position.

5. The PCR detection device of claim 4, wherein: The fixing buckle (11) comprises a first supporting piece (1101) and a hook (1102), the supporting piece (1101) is fixedly arranged on the sample rack (9), and a second supporting piece (1305) is fixedly arranged below the heating pipe (12).

6. The PCR detection device of claim 5, wherein: A fan (13) is arranged below the second supporting piece, a connecting piece (1301) is arranged in the second supporting piece (1305), a fan blade (1302) is arranged below the connecting piece (1301), the fan blade (1302) is arranged on a rotating shaft (1303) below the fan blade (1302), a motor (1304) is arranged below the rotating shaft (1303), and the motor (1304) drives the rotating shaft (1303) to rotate.

7. The PCR detection device of claim 6, wherein: A photoelectric detector (15) is fixedly arranged below the motor (1304), a plurality of grooves are arranged in the photoelectric detector (15), a filter (14) is fixedly arranged in the groove, and an optical lens system is fixedly arranged in the photoelectric detector (15) and close to the filter (14).

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