Liquid-based cell dyeing and flaking all-in-one machine

Abstract

The utility model discloses a liquid-based cell staining and slide-making all-in-one machine, which relates to the field of cell staining and slide-making, and comprises a liquid-based cell staining and slide-making machine, a slide-making machine side shell, a bottom inner cavity, a top inner cavity, a centrifugal disc body, a sample disc body and a slide-making machine body, a staining reagent bottle is arranged in the liquid-based cell staining tableting machine, and a staining reagent replacement bottle can be selected as the staining reagent bottle. The liquid-based cell staining tableting machine solves the problem that liquid cannot be added and discharged due to adhesion of a pipeline after corrosion caused by absence of water and waste liquid; and the high-precision sample needle is matched with the ceramic pump to accurately control the sample adding amount of a dyeing reagent and a sample by adopting a dripping dyeing technology. The sample adding needle and the dyeing needle both adopt a drop dyeing technology, so that a traditional injector sample adding mode is abandoned, the problem that the needle head of the sample adding needle is blocked by crystals due to the fact that a dyeing reagent is not used for a long time is fundamentally solved, and the dyeing effect is better.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell dyeing section field, specifically is a liquid based cell dyeing section integrated machine. BACKGROUND

[0002] The basic principle of microbial dyeing is carried out by means of physical factors and chemical factors. Physical factors such as capillary phenomenon, penetration, adsorption of cells and cell substances on dyes. Chemical factors are various chemical reactions that occur according to the different properties of cell substances and dyes. Acidic substances are more easily adsorbed by basic dyes, and the adsorption is stable; similarly, basic substances are more easily adsorbed by acidic dyes. For example, the nucleus of acidic substances has chemical affinity for basic dyes and is easily adsorbed. However, in order to dye acidic materials with acidic substances, their physical form must be changed to facilitate the occurrence of adsorption. On the contrary, basic substances can only be dyed with acidic dyes, and if they are changed to suitable physical forms, they can also have adsorption with basic dyes.

[0003] The traditional liquid based cell dyeing section integrated machine has the problems of sample bottle jumping out of the sample disc when taking samples, sample needle blockage, pipe loosening caused by blockage, the need to reopen the mold for the centrifugal disc basket, and the problems of basket breakage, fragmentation, and insufficient angle during centrifugation, especially the increased probability of positive and negative pieces, dyeing overlap caused by insufficient centrifugation angle, and corrosion and adhesion of the reagent pipeline caused by lack of water during cleaning and fixation, which is difficult to prolong the service life of the pipeline.

[0004] Therefore, the skilled person in the art provides a liquid based cell dyeing section integrated machine to solve the problems raised in the background. UTILITY MODEL CONTENTS

[0005] The utility model aims at providing a liquid based cell dyeing section integrated machine to solve the problems of the traditional liquid based cell dyeing section integrated machine in use, such as sample bottle jumping out of the sample disc when taking samples, sample needle blockage, pipe loosening caused by blockage, the need to reopen the mold for the centrifugal disc basket, and the problems of basket breakage, fragmentation, and insufficient angle during centrifugation, especially the increased probability of positive and negative pieces, dyeing overlap caused by insufficient centrifugation angle, and corrosion and adhesion of the reagent pipeline caused by lack of water during cleaning and fixation, which is difficult to prolong the service life of the pipeline.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme:

[0007] A liquid-based cell staining and sheet making all-in-one machine, comprising a liquid-based cell staining and sheet making machine, a sheet making machine side shell, a bottom inner cavity, a top inner cavity, a centrifugal disc disc body, a sample disc disc body and a sheet making machine body, the top surface of the liquid-based cell staining and sheet making machine is provided with a pull-out machine cover, the liquid-based cell staining and sheet making machine is internally provided with a staining reagent bottle, the staining reagent bottle can also be replaced with a staining reagent replacement bottle, one side of the liquid-based cell staining and sheet making machine is externally connected with an external water bucket A and an external water bucket B, the liquid-based cell staining and sheet making machine is internally provided with a centrifugal disc and a sample disc, the top of the sheet making machine body is provided with a fixed top shell, one side of the fixed top shell is rotationally connected with a turnover plate, the top of the sheet making machine body is internally provided with a top inner cavity, the bottom of the sheet making machine body is internally provided with a bottom inner cavity, the front side of the sheet making machine body is provided with a sheet making machine side shell, one side of the sheet making machine body is provided with a side edge cleaner, the top plate on the inner side of the sheet making machine body is provided with a tube taking and placing device, a cleaning tube and a sample adding needle, the inside of the centrifugal disc disc body is provided with a centrifuge, the bottom end of the centrifugal disc disc body is sleeved with a transmission belt A, one end of the transmission belt A is sleeved with a driving motor A, the inside of the centrifugal disc disc body is provided with a centrifuge and a hanging basket, the inside of the sample disc disc body is provided with a sample placing tube, the bottom end of the sample disc disc body is provided with a rotating shaft, the bottom end of the rotating shaft is sleeved with a transmission belt B, one end of the transmission belt B is sleeved with a driving motor B.

[0008] As a preferred embodiment of the present application: the liquid-based cell staining and sheet making machine comprises a sheet making machine side shell, a fixed top shell, a turnover plate, a bottom inner cavity, a top inner cavity, a side edge cleaner and a sheet making machine body.

[0009] As a preferred embodiment of the present application: the centrifugal disc comprises a tube taking and placing device, a cleaning tube, a centrifugal disc disc body, a transmission belt A, a driving motor A, a centrifuge and a hanging basket.

[0010] As a preferred embodiment of the present application: the tube taking and placing device, the cleaning tube and the sample adding needle are located inside the top inner cavity.

[0011] As a preferred embodiment of the present application: the hanging basket is provided with a plurality of hanging baskets, and the hanging baskets are uniformly distributed around the centrifuge.

[0012] As a preferred embodiment of the present application: the sample disc comprises a driving motor B, a transmission belt B, a rotating shaft, a sample disc disc body, a sample placing tube and a sample adding needle.

[0013] As a preferred embodiment of the present application: a communication pipeline is arranged between the centrifugal disc and the sample disc, the communication pipeline is selected from a Teflon pipeline, and a fluorine-containing diaphragm pump and an electromagnetic valve are arranged between the communication pipelines.

[0014] Compared with the prior art, the present application has the following advantages:

[0015] The utility model discloses a liquid-based cell dyeing and sheet making integrated machine, sample disc bottom adds 8mm, solved the sample bottle when sampling needle bottle body jump skew, jump out sample disc problem, solved the sample needle block needle problem, solved the block needle caused pipeline loosening problem, centrifugal disc basket reopens the mould upgrade, solved the basket breakage, broken problem, solved the angle not enough problem when centrifuging, the probability of yin and yang sheet, solved the dyeing overlap problem caused by the centrifugal angle not enough, the reagent pipeline of washing and fixation has all been replaced with teflon pipeline, the corrosion adhesion problem caused by anhydrous is eliminated, prolongs the pipeline service life, more beautiful and neat simultaneously, is convenient for distinguishing, lets the customer not be confused, replaced the separation liquid, pure water and waste liquid peristaltic pump, waste liquid and pure water have been replaced with fluorine-containing diaphragm pump, anhydrous, buffer solution and separation liquid have been replaced with electromagnetic valve combination fluorine-containing diaphragm pump, completely solved the pipeline corrosion after adhesion problem caused by anhydrous and waste liquid, high-precision sample needle cooperates with ceramic pump and adopts drop dyeing technology to accurately control the sample amount of dyeing reagent and sample, adds sample and adds dyeing reagent, and the dyeing needle all adopts the technology of drop dyeing, discards the traditional syringe sample mode, fundamentally solves the crystallization blockage sample needle problem caused by dyeing reagent long time not using, and the dyeing effect is better. BRIEF DESCRIPTION OF DRAWINGS

[0016] Other features, objects, and advantages of the utility model will become more apparent from the following detailed description of non-limiting embodiments, with reference to the drawings:

[0017] Figure 1 It is a kind of liquid-based cell dyeing and sheet making integrated machine's whole three-dimensional structure schematic diagram;

[0018] Figure 2 It is the internal structure schematic diagram of the whole in a kind of liquid-based cell dyeing and sheet making integrated machine;

[0019] Figure 3 It is the internal structure schematic diagram of the connecting portion of centrifugal disc and sample disc in a kind of liquid-based cell dyeing and sheet making integrated machine;

[0020] Figure 4 It is the structure schematic diagram of the top surface of centrifugal disc and sample disc in a kind of liquid-based cell dyeing and sheet making integrated machine.

[0021] Figure 5 It is the three-dimensional structure schematic diagram of external connection water bucket in a kind of liquid-based cell dyeing and sheet making integrated machine.

[0022] Figure 6 It is the three-dimensional structure schematic diagram of dyeing reagent replacement bottle in a kind of liquid-based cell dyeing and sheet making integrated machine.

[0023] In the figure: 1, liquid-based cell staining machine; 101, side shell of the machine; 102, fixed top shell; 103, turnover plate; 104, bottom inner cavity; 105, top inner cavity; 106, side cleaner; 107, machine body; 2, puller cover; 3, centrifugal disc; 301, tube taker; 302, cleaning tube; 303, disc body; 304, transmission belt A; 305, driving motor A; 306, centrifugal machine; 307, hanging basket; 4, sample disc; 401, driving motor B; 402, transmission belt B; 403, rotating shaft; 404, disc body; 405, sample placement tube; 406, sample adding needle; 5, staining reagent bottle; 6, external water bucket A; 7, external water bucket B; 8, staining reagent replacement bottle. DETAILED DESCRIPTION

[0024] Please refer to Figures 1-6The utility model discloses an embodiment of a kind of liquid-based cell dyeing and making sheet integrated machine, including liquid-based cell dyeing and making sheet machine 1, sheet machine side shell 101, bottom inner chamber 104, top inner chamber 105, centrifugal disc disc body 303, sample disc disc body 404 and sheet machine body 107, the top surface of liquid-based cell dyeing and making sheet machine 1 is equipped with puller cover 2, be equipped with dyeing reagent bottle 5 in liquid-based cell dyeing and making sheet machine 1, dyeing reagent bottle 5 also can be selected dyeing reagent replacement bottle 8, one side of liquid-based cell dyeing and making sheet machine 1 is externally connected with external water bucket A 6 and external water bucket B 7, centrifugal disc 3 and sample disc 4 are equipped in liquid-based cell dyeing and making sheet machine 1, when using, can be communicated by external water bucket A 6 and external water bucket B 7, then by drive motor B 401 drive, transmission belt B 402 and rotating shaft 403 can be driven to rotate, then by sample disc disc body 404 sample placement tube 405 is placed, simultaneously can realize rotation, the top of sheet machine body 107 is equipped with fixed top shell 102, one side of fixed top shell 102 is rotatably connected with turnover plate 103, the top of sheet machine body 107 is equipped with top inner chamber 105, the bottom of sheet machine body 107 is equipped with bottom inner chamber 104, the front side of sheet machine body 107 is equipped with sheet machine side shell 101, one side of sheet machine body 107 is equipped with side edge cleaner 106, sample disc bottom is added 8mm, solve the problem that sample bottle is taken sample and is stuck needle bottle body jump skew, jump out sample disc, solve the problem that sample needle is blocked needle, solve the problem that blocked needle causes pipeline to loosen, centrifugal disc basket is re-opened and upgraded, solve the problem of basket breakage, broken, can be convenient for sample needle 406 to sample, drive motor A 305 can play the role of driving centrifugal disc disc body 303, transmission belt A 304 can play the transmission effect, facilitate centrifuge 306 and basket 307 rotation, centrifugal disc basket is re-opened and upgraded, solve the problem of basket breakage, broken, solve the problem of insufficient angle when centrifuging, the probability of yin and yang sheet, solve the problem of dyeing overlap caused by insufficient centrifugal angle, the inside top plate of sheet machine body 107 is equipped with take tube placer 301, cleaning tube 302 and sample needle 406, the inside of centrifugal disc disc body 303 is equipped with centrifuge 306, the bottom of centrifugal disc disc body 303 is sleeved with transmission belt A 304, one end of transmission belt A 304 is sleeved with drive motor A 305, the inside of centrifugal disc disc body 303 is equipped with centrifuge 306 and basket 307, the inside of sample disc disc body 404 is equipped with sample placement tube 405, the bottom of sample disc disc body 404 is equipped with rotating shaft 403, the bottom of rotating shaft 403 is sleeved with transmission belt B 402, one end of transmission belt B 402 is sleeved with drive motor B 401.

[0025] Please refer to Figure 1 、 Figure 3 And Figure 6The liquid-based cell staining and sheet making machine 1 comprises a sheet maker side shell 101, a fixed top shell 102, a turnover plate 103, a bottom inner cavity 104, a top inner cavity 105, a side cleaner 106 and a sheet maker body 107. The communication pipes between the centrifugal disc 3 and the sample disc 4 are made of Teflon pipes, and fluorine-containing diaphragm pumps and electromagnetic valves are arranged between the communication pipes. The reagent pipes for cleaning and fixing are all replaced by Teflon pipes, so that the problem of corrosion and adhesion caused by water is eliminated, the service life of the pipes is prolonged, the appearance is more beautiful and neat, and the customer is not confused. The centrifugal disc 3 comprises a pipe taking and placing device 301, a cleaning pipe 302, a centrifugal disc body 303, a transmission belt A 304, a driving motor A 305, a centrifuge 306 and a hanging basket 307. The pipe taking and placing device 301, the cleaning pipe 302 and the sample adding needle 406 are located inside the top inner cavity 105. The hanging basket 307 is provided with a plurality of hanging baskets 307, and the hanging baskets 307 are uniformly distributed around the centrifuge 306. The peristaltic pump for replacing the separation liquid, the pure water and the waste liquid is replaced by the fluorine-containing diaphragm pump, the waste liquid and the pure water are replaced by the fluorine-containing diaphragm pump, the water, the buffer and the separation liquid are replaced by the electromagnetic valve combined with the fluorine-containing diaphragm pump, and the problem of pipe corrosion and adhesion caused by water and waste liquid is completely solved. The high-precision sample needle cooperates with the ceramic pump to adopt the drop dyeing technology to accurately control the sample adding amount of the dyeing reagent and the sample. The sample adding needle and the dyeing needle all adopt the drop dyeing technology, and the traditional syringe sample adding mode is abandoned, so that the problem of crystallization blockage of the sample adding needle caused by long-term non-use of the dyeing reagent is fundamentally solved, and the dyeing effect is better. The sample disc 4 comprises a driving motor B 401, a transmission belt B 402, a rotating shaft 403, a sample disc body 404, a sample placing pipe 405 and a sample adding needle 406. The communication pipes between the centrifugal disc 3 and the sample disc 4 are made of Teflon pipes, and fluorine-containing diaphragm pumps and electromagnetic valves are arranged between the communication pipes.

[0026] It should be noted that the utility model is a kind of liquid-based cell staining and sheet making machine, including 1, liquid-based cell staining and sheet making machine;101, sheet maker side shell;102, fixed top shell;103, turnover plate;104, bottom inner cavity;105, top inner cavity;106, side cleaner;107, sheet maker body;2, pull machine cover;3, centrifugal disc;301, take pipe placing device;302, cleaning pipe;303, centrifugal disc body;304, transmission belt A;305, driving motor A;306, centrifuge;307, hanging basket;4, sample disc;401, driving motor B;402, transmission belt B;403, rotating shaft;404, sample disc body;405, sample placing pipe;406, sample adding needle;5, dyeing reagent bottle;6, external water bucket A;7, external water bucket B;8, dyeing reagent replacement bottle, components are general standard parts or components known to those skilled in the art, and the structure and principle are known to those skilled in the art by technical manual or by conventional experimental method.

[0027] The working principle of the utility model is: when using, external water bucket A6 and external water bucket B7 can be communicated, then drive motor B401 is driven, transmission belt B402 and rotating shaft 403 can be driven to rotate, then sample placing tube 405 is placed through sample disc body 404, and rotation can be realized, the sample disc bottom is increased by 8mm, the problems that the sample bottle is skewed and jumps out of the sample disc when sampling are solved, the problem that the sample needle is blocked is solved, the problem that the pipeline is loosened caused by the blocked needle is solved, the centrifugal disc basket is re-opened and upgraded, the problems that the basket is broken and crushed are solved, the sample needle 406 can be conveniently used for sampling, drive motor A305 can drive the centrifugal disc body 303, transmission belt A304 can realize transmission effect, the centrifuge 306 and the basket 307 can be conveniently rotated, the centrifugal disc basket is re-opened and upgraded, the problems that the basket is broken and crushed are solved, the problem that the angle is not enough when centrifuging is solved, the probability of the male and female pieces is solved, the problem that the dyeing overlaps caused by the insufficient centrifugal angle is solved, the communication pipeline between the centrifugal disc 3 and the sample disc 4 selects a Teflon pipeline, fluorine-containing diaphragm pumps and electromagnetic valves are arranged between the communication pipelines, the reagent pipeline for cleaning and fixing is all replaced by a Teflon pipeline, the problem of corrosion and adhesion caused by no water is eliminated, the service life of the pipeline is prolonged, and the pipeline is more beautiful and neat, so that customers are not confused, the peristaltic pump for replacing the separating liquid, pure water and waste liquid is replaced, the waste liquid and the pure water are replaced by fluorine-containing diaphragm pumps, the no water, buffer solution and separating liquid are replaced by electromagnetic valves combined with fluorine-containing diaphragm pumps, the problem that the pipeline is corroded and adhered and cannot be filled and drained due to no water and waste liquid is completely solved, the high-precision sample needle cooperates with the ceramic pump to adopt drop dyeing technology to accurately control the sample amount of the dyeing reagent and the sample. The sample needle and the dyeing needle all adopt drop dyeing technology, and the traditional syringe sampling mode is abandoned, so that the problem of crystallization blockage of the sample needle caused by long-time non-use of the dyeing reagent is fundamentally solved, and the dyeing effect is better.

[0028] The above is only a preferred specific embodiment of the utility model, but the protection scope of the utility model is not limited to this, any skilled person in the art can make equivalent replacement or change according to the technical scheme and the utility model concept of the utility model within the technical range disclosed by the utility model, which should be covered in the protection scope of the utility model.

Claims

1. A liquid-based cell staining and slide making all-in-one machine, comprising a liquid-based cell staining and slide making machine (1), a slide making machine side shell (101), a bottom inner cavity (104), a top inner cavity (105), a centrifugal disc disc body (303), a sample disc disc body (404) and a slide making machine body (107), characterized in that, The top surface of the liquid-based cell staining machine (1) is provided with a pull-out cover (2), the liquid-based cell staining machine (1) is provided with a staining reagent bottle (5), the staining reagent bottle (5) can also be replaced by a staining reagent replacement bottle (8), one side of the liquid-based cell staining machine (1) is externally connected with an external water bucket A (6) and an external water bucket B (7), the liquid-based cell staining machine (1) is provided with a centrifugal disc (3) and a sample disc (4), the top of the machine body (107) is provided with a fixed top shell (102), one side of the fixed top shell (102) is rotatably connected with a turnover plate (103), the top of the machine body (107) is provided with a top inner cavity (105), the bottom of the machine body (107) is provided with a bottom inner cavity (104), the front side of the machine body (107) is provided with a machine side shell (101), one side of the machine body (107) is provided with a side cleaning device (106), the top plate on the inner side of the machine body (107) is provided with a tube taking and placing device (301), a cleaning tube (302) and a sample adding needle (406), the inside of the centrifugal disc disc body (303) is provided with a centrifugal machine (306), the bottom end of the centrifugal disc disc body (303) is sleeved with a transmission belt A (304), one end of the transmission belt A (304) is sleeved with a driving motor A (305), the centrifugal disc disc body (303) is provided with a centrifugal machine (306) and a hanging basket (307), the inside of the sample disc disc body (404) is provided with a sample placing tube (405), the bottom end of the sample disc disc body (404) is provided with a rotating shaft (403), the bottom end of the rotating shaft (403) is sleeved with a transmission belt B (402), one end of the transmission belt B (402) is sleeved with a driving motor B (401).

2. The liquid-based cell staining and slide making all-in-one machine according to claim 1, wherein, The liquid-based cell staining machine (1) comprises a machine side shell (101), a fixed top shell (102), a turnover plate (103), a bottom inner cavity (104), a top inner cavity (105), a side cleaning device (106) and a machine body (107).

3. The liquid-based cell staining and slide making all-in-one machine of claim 1, wherein, The centrifugal disc (3) comprises a tube taking and placing device (301), a cleaning tube (302), a centrifugal disc disc body (303), a transmission belt A (304), a driving motor A (305), a centrifugal machine (306) and a hanging basket (307).

4. The liquid-based cell staining and slide making all-in-one machine of claim 1, wherein, The tube taking and placing device (301), the cleaning tube (302) and the sample adding needle (406) are located inside the top inner cavity (105).

5. The liquid-based cell staining and slide making all-in-one machine of claim 1, wherein, The hanging basket (307) is provided with a plurality of hanging baskets (307), and the hanging baskets (307) are uniformly distributed around the centrifugal machine (306).

6. The liquid-based cell staining and slide making all-in-one machine of claim 1, wherein, The sample disc (4) comprises a driving motor B (401), a transmission belt B (402), a rotating shaft (403), a sample disc disc body (404), a sample placing tube (405) and a sample adding needle (406).

7. The liquid-based cell staining and slide making all-in-one machine of claim 1, wherein, The centrifugal disc (3) and the sample disc (4) are provided with a communication pipeline, the communication pipeline is selected from a Teflon pipeline, and the communication pipelines are provided with a fluorine-containing diaphragm pump and an electromagnetic valve.

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